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At least 37 records · Page 2Linked to original sources
USGS2020

Distribution and transport of Olympia oyster, Ostrea lurida, larvae in northern Puget Sound, Washington, USA

As efforts for restoring Olympia oyster (Ostrea lurida) populations have expanded, there is an increased need to understand local factors that could influence the long-term success of these projects. To address concerns over potential limitations to recruitment at a restoration site in northern Puget Sound, Washington, USA, a study was developed to characterize physical processes governing larval transport in conjunction with larval abundance and environmental factors. Larval presence was not associated with tide cycle, season, or a combination of tide cycle and season. In terms of location, larvae were more likely to be present at offshore and intertidal sites versus the estuarine lagoon where the adult population resides. Larval density was higher during late summer ebbs versus early summer floods. Across sampling dates and locations, larval sizes ranged from 184 to 263 µm, indicating that larvae were released into the water column throughout the reproductive season and retained in the embayment for at least ~16 days. Throughout different tidal cycles in Skagit Bay, acoustic Doppler current profilers were used to measure current direction and velocities, concurrent with plankton sampling. Surface currents in the study area alternated between a clockwise and counterclockwise gyre during initial ebb and flood tides, respectively. Larvae exported from the source population during initial to mid-ebbs are swept into a northward gyre, and potentially retained at intertidal sites alongshore. These results will provide resource managers attempting to restore native bivalves with the ability to expand populations by identifying optimal areas for habitat enhancement through natural recruitment.

Washington↗
USGS2023

Hypoxia and anoxia tolerance in diploid and triploid eastern oysters at high temperature

Increasing reliance on the use of triploid oysters to support aquaculture production relies on their generally superior growth rate and meat quality over that of diploid oysters. Reports of elevated triploid mortality have generated questions about potential trade-offs between growth and tolerance to environmental stressors. These questions are particularly relevant as climate change, coastal activities, and river management impact water salinity, temperature, nutrients, pH, and oxygen levels within key estuarine oyster growing areas. In particular, the co-occurrence of warm water temperatures and low dissolved oxygen concentration (DO) events are increasingly reported in estuaries, with potentially lethal impacts on sessile, oyster resources. To investigate potential differences in DO tolerance, diploid and triploid market-sized or seed oysters were exposed to continuous normoxia (DO > 5.0 mg L –1 ), hypoxia (DO < 2.0 mg L –1 ), and anoxia (DO < 0.5 mg L –1 ) at 28°C and their mortalities were monitored. The hemolymph of the market-sized oysters was collected to measure cellular and biochemical changes in response to hypoxia and anoxia, whereas their valve movements were also measured. In general, about half of market-sized oysters died within about 1 wk under anoxia (LT 50 : 5.7–8.9 days) and within about 2 wk under hypoxia (LT 50 : 11.9–19.4 days) with diploid oysters tending to die faster than triploid oysters. Seed oysters took longer to die than market-sized oysters under both anoxia (LT 50 : 9.5–12.1 days) and hypoxia (LT 50 : 21.8–25.0 days) with diploid oysters (LT 50 : 9.5–11.8 days) dying slightly faster than triploid oysters (LT 50 : 11.8–12.1 days) under anoxia. Hemolymph pH decreased and plasma calcium and glutathione concentrations increased with decreasing DO, with values under anoxia being different than those under normoxia. Hemocyte density was also lower under anoxia than under either normoxia or hypoxia. Overall, few differences in physiological responses to hypoxia and anoxia were found between diploid and triploid oysters suggesting that ploidy (2N versus 3N) had limited effect on the tolerance and response of eastern oysters to low DO.

Journal of Shellfish Research↗
USGS2025

Vulnerability of gulf ribbed mussels to marsh surface maximum temperatures

Gulf ribbed mussels ( Geukensia granosissima ) act as ecosystem engineers and reside within the marsh platform of saltmarshes across the northern Gulf of Mexico. With climate models projecting increasing temperatures, and more frequent and extreme heat events, these mussels face increasing temperature-related risks. Marsh surface and subsurface (5-cm depth) temperature was measured continuously in the summer of 2022 in south Louisiana Gulf ribbed mussel habitat at nine stations. Marsh surface maximum temperatures were 5°C higher and more variable than recorded water temperatures, exceeding 38°C for periods of up to 3 h which generally coincided with low tides and peak solar radiation. Marsh subsurface temperatures were cooler with a lower mean and maximum temperature compared with the marsh surface, but higher than adjacent water. In two laboratory experiments the acclimated and acute thermal tolerance of wild mussels collected from the saltmarsh where temperatures were recorded, were explored. G. granosissima survived more than 40 days of continuous exposure in the laboratory to mean daily temperature values recorded for the marsh and subsurface microhabitats (28°C–34°C) but their calculated median lethal time (LT 50 ) ranged from 35 to 56 days (36°C), to less than 3 days (40°C). Mussels acclimated to temperatures similar to long-term average water temperatures (28°C–32°C) and then exposed to maximum daily temperatures acutely experienced LT 50 of less than 6 days (38°C), <1 day (40°C), and of less than 5 h (42°C). For G. granosissima both their thermal tolerance and behavioral response likely contribute to their survival in the face of extreme heat events, and their resulting distribution across the marsh surface and subsurface. Overall, results indicate that ribbed mussels in coastal Louisiana may rely on their ability to migrate vertically and bury in the marsh to avoid extreme heat exposure (temperature, duration) that may be lethal. The ability of Gulf ribbed mussels to endure short-term thermal extremes may ultimately determine the mussels' use as a tool in marsh stabilization and coastal restoration.

Louisiana↗
USGS2005

Cryopreservation of sperm of red abalone (Haliotis rufescens)

Abalone culture, a developing industry in Baja California, Mexico, would benefit from genetic improvement and controlled breeding. The use of cryopreserved sperm would allow germplasm availability, and this study was designed to develop sperm cryopreservation protocols for red abalone Haliotis rufescens . The acute toxic effects of the cryoprotectants dimethyl sulfoxide (DMSO), propylene glycol (PG), and glycerol (GLY) were assessed after suspending sperm in different concentrations, whereby cryoprotectant treatments of 10% DMSO and 10% GLY equilibrated for 10 min yielded the highest range of motile sperm in preliminary freezing trials and were used for cryopreservation studies. To determine effective cooling rates, three freezing chambers were tested. Replicate samples of sperm from 4 males were placed in 0.5-mL French straws and frozen using a commercial freezing chamber (CFC) used for bull sperm, a programmable rate chamber (PRC), and a manually controlled styrofoam chamber (MCC). For the CFC, the cooling rate was 16°C/min, from 4°C to −140°C. For the PRC and MCC, it was 1°C/min, from −20°C to −30°C. The samples were held at −30°C for 5 min before being plunged into liquid nitrogen (−196°C) for storage, and each sample was thawed in a water bath at 45°C for 8 s. The quality of thawed sperm was determined by estimating percent motility, evaluating membrane integrity using a dual-staining technique and flow cytometry, and estimating fertilization rate. Statistical analyses were performed using 2-way ANOVA where chamber and treatment were the independent variables. Sperm quality parameters were independent. For motilities, a significant interaction was noted between the cryoprotective treatment and the chamber type, whereby motilities for DMSO and GLY were higher ( P = 0.0055) using MCC. Membrane integrities were significantly lower after using the PRC than the CFC or the MCC ( P = 0.0167). The highest post-thaw motility (48 ± 7%) was found using sperm suspended in 10% glycerol and frozen in the MCC. The highest percent of intact membranes (56 ± 11%) was for sperm suspended in 10% glycerol and frozen in the CFC. The highest fertilization rate (29 ± 10%) was with samples frozen with 10% glycerol in the CFC. The use of cryopreserved sperm from red abalone provides an alternative breeding option for culture and the protocols delineated are the first developed for this species.

Baja California↗
USGS2005

Quarantine of Aeromonas salmonicida-harboring ebonyshell mussels (Fusconaia ebena) prevents transmission of the pathogen to brook trout (Salvelinus fontinalis)

Furunculosis, caused by the bacterium Aeromonas salmonicida , was artificially induced in brook trout ( Salvelinus fontinalis ) in an experimental tank. Ebonyshells ( Fusconaia ebena ) were placed to cohabit with these fish to acquire the pathogen through siphoning. After 2 wk of cohabitation, 10 of the mussels were assayed by bacterial culture and all were found to harbor A. salmonicida . The mean cell count from soft tissue homogenates was 1.84 × 10 5 cfu/g, which comprised an average 14.41% of the total bacteria isolated from tissues. From the fluids, a mean of 2.84 × 10 5 A. salmonicida cfu/mL was isolated, which comprised an average of 17.29% of the total bacterial flora. The mussels were removed from the cohabitation tank and distributed equally among five previously disinfected tanks, 35 per tank. The F. ebena in each tank were allowed to depurate A. salmonicida for various durations: 1, 5, 10, 15 or 30 days. After each group had depurated for their assigned time, 10 were assayed for bacteria, tank water was tested, and 20 pathogen-free bioindicator brook trout were added to cohabit with the remaining mussels. Depuration was considered successful if A. salmonicida was not isolated from tank water or the mussels, and there was no infection or mortality to bioindicator fish. After 1 day of depuration, A. salmonicida was not isolated from the soft tissues; however, it was isolated from one of the paired fluids (10% prevalence). The tank water tested positive, and the bioindicator fish became infected and died. From the 5-day depuration group, A. salmonicida was not isolated from soft tissues, but was isolated from three fluids (30%; mean = 1.56 × 10 2 cfu/mL). Tank water from the 5-day group was negative, and there was no mortality among the bioindicator fish. However, A. salmonicida was isolated from 2 of 20 fish at the end of the 14-day observation period. One F. ebena fluid sample was positive for A. salmonicida from the 10-day depuration group, but none of the soft tissue homogenates. The pathogen was not isolated from 10-day tank water, but there was a 30% cumulative mortality to the bioindicator fish. Aeromonas salmonicida was not isolated from any of the soft tissue homogenates, fluids or tank water from the 15 day or 30 day depuration groups, and the bioindicator fish remained pathogen- and disease-free. Study results showed that the F. ebena were harboring a high A. salmonicida cell load going into depuration, but at 15 days and beyond, the pathogen had been depurated to the extent that the mussels did not serve as pathogen vectors.

Journal of Shellfish Research↗
USGS2005

Acute toxicity of resmethrin, malathion and methoprene to larval and juvenile American lobsters ( Homarus amemcanus ) and analysis of pesticide levels in surface waters after Scourge™, Anvil™ and Altsoid™ application

Acute toxicity and immune response, combined with temperature stress effects, were evaluated in larval and juvenile American lobsters ( Homarus americanus ) exposed to malathion, resmethrin and methoprene. These pesticides were used to control West Nile virus in New York in 1999, the same year the American lobster population collapsed in western Long Island Sound (LIS). Whereas the suite of pesticides used for mosquito control changed in subsequent years, a field study was also conducted to determine pesticide concentrations in surface waters on Long Island and in LIS after operational applications. The commercial formulations used in 2002 and 2003&mdash;Scourge, Anvil and Altosid&mdash;contain the active ingredients resmethrin, sumithrin and methoprene, respectively. Concentrations of the synergist piperonyl butoxide (PBO) were also measured as a proxy for pesticide exposure. Acute mortality in Stage I-II larval lobsters demonstrated that they are extremely sensitive to continuous resmethrin exposure. Resmethrin LC50s for larval lobsters determined under flow-through conditions varied from 0.26&ndash;0.95 &mu;g L &minus;1 in 48- and 96-h experiments at 16&deg;C, respectively. Increased temperature (24&deg;C) did not significantly alter resmethrin toxicity. Malathion and methoprene were less toxic than resmethrin. The 48-h LC50 for malathion was 3.7 &mu;g L &minus;1 and methoprene showed no toxicity at the highest (10 &mu;g L &minus;1 ) concentration tested. Phenoloxidase activity was used as a measure of immune response for juvenile lobsters exposed to sublethal pesticide concentrations. In continuous exposures to sublethal doses of resmethrin (0.03 &mu;g L &minus;1 ) or malathion (1 &mu;g L &minus;1 ) for 7 d at 16 or 22&deg;C, temperature had a significant effect on phenoloxidase activity ( P &le; 0.006) whereas pesticide exposure did not ( P = 0.880). The analytical methods developed using high performance liquid chromatography coupled to time-of-flight mass spectroscopy (LC-TOF-MS) provided high sensitivity with mass detection limits of 0.1&ndash;0.3 ng L &minus;1 . Pesticide levels were often detected in the ng L &minus;1 range in Long Island surface waters and western LIS (except in open waters), but rarely at concentrations found to be toxic in flow-through laboratory exposures, even immediately after spray events.

Journal of Shellfish Research↗
USGS2008

Recovery of a fish pathogenic bacterium, Aeromonas salmonicida, from ebonyshell mussels Fusconaia ebena using nondestructive sample collection procedures

Refugia are increasingly being used to maintain and propagate imperiled freshwater mussels for future population augmentations. Success for this endeavor is dependent on good husbandry, including a holistic program of resource health management. A significant aspect to optimal health is the prevention or control of infectious diseases. Describing and monitoring pathogens and diseases in mussels involves examination of tissues or samples collected from an appropriate number of individuals that satisfies a certain confidence level for expected prevalences of infections. In the present study, ebonyshell mussels Fusconaia ebena were infected with a fish pathogenic bacterium, Aeromonas salmonicida, through their cohabitation with diseased brook trout Salvelinus fontinalis. At a 100% prevalence of infection, the F. ebena were removed from the cohabitation tank to clean tanks that were supplied with pathogen-free water, which initiated their depuration of A. salmonicida. Three samples (nondestructive fluid, mantle, hemolymph) collected using nondestructive procedures were compared with fluids and soft tissue homogenates collected after sacrificing the mussels for recovery of the bacterium during this period of depuration. Nondestructive sample collections, especially ND fluid, provide a comparable alternative to sacrificing mussels to determine pathogen status.

Journal of Shellfish Research↗
USGS1994

A bibliography of "Dreissena polymorpha in European and Russian waters: 1964-1993"

A bibliography of over 1000 papers on the biology, impacts, and control of the zebra mussel (Dreissena polymorpha) in European and Russian waters is compiled to aid scientists and managers in addressing this species of economic and ecological importance. The bibliography primarily includes publications between the early 1960s and early 1990s but does contain some earlier references not found in another extensive bibliography published in 1964. This bibliography will be a valuable tool, especially to water users and environmental scientists in North America where zebra mussels have recently invaded and become established.

Journal of Shellfish Research↗
USGS2001

Lack of surface-associated microorganisms in a mixed species community of freshwater Unionidae

To determine whether unionids contain surface-attached endosymbiotic bacteria, ciliates, or fungi, we used scanning electron microscopy to examine the epithelial surface of various organs within the digestive systems and mantle cavity of temperate river and lake unionids on a seasonal basis. We also cultured material removed from the lumen of these same organs and from the mantle cavity to detect cellobiose-, cellulose-, and chitin- degrading microbes. No true endosymbiotic fauna were observed attached to the surface of the digestive or mantle tissues of any species of unionid. Microbial growth on cellulose or chitin bacteriological media varied by season and habitat, but not by unionid species or source of the isolate. Lake unionids did not contain microbes capable of digesting cellulose or chitin, whereas unionids from the river site did in March and August, but not in December. Since these cultured cellulose- and chitin-degrading bacteria were never found attached to any unionid tissues, they appeared to be transient forms, not true endosymbionts. Microbes capable of digesting cellobiose were found in every unionid collected in all seasons and habitats, but again, no microbes were directly observed attached to unionid tissues. If unionids, like most other vertebrates, lack digestive enzymes required to initiate primary bond breakage, then the lack of cellulolytic and chitinolytic endosymbionts would affect the ability to utilize cellulose or chitin foods. Thus, in captivity dry feeds based on corn, soybeans, or nauplii should be pre-digested to ensure maximum absorption of nutrients by unionids. The lack of celluloytic or chitinolytic endosymbionts should not affect relocation success, though the seasonal role of transient microbes in unionid nutrition requires further investigation.

Journal of Shellfish Research↗
USGS2000

Emersion and thermal tolerances of three species of unionid mussels: Survival and behavioral effects

We evaluated the behavior and survival of unionid mussels after emersion in air temperatures across a range that is likely to be encountered during status surveys or relocations. Five laboratory tests were performed with pocketbook Lampsilis cardium Rafinesque (2 tests), pimpleback Quadrula pustulosa Lea (1 test), and spike Elliptio dilatata Rafinesque (2 tests) mussels, each conducted in a completely randomized, nested experimental design. For each mussel species (except Q. Pustulosa), treatments tested included two water temperatures (25 and 10 degrees C), five air temperatures (ranging within +/-20 degrees C of the water temperature), three aerial exposure durations (15, 30, and 60 min), and a no emersion control. All treatments were duplicated, with 10 organisms per emersion time and aerial exposure temperature (n = 320 mussels per test). Behavioral response (ability to upright) and mortality were measured daily for 14 d postemersion. Both water and aerial exposure temperature (air shock) were important predictors of times to first uprighting. The intensity function of first uprighting differed among species (P < 0.01), and there was a significant interaction between E. Dilatata versus the other species and water temperature (P +/- 0.01). Over-all mussel survival after emersion was high (93%); however, E. Dilatata experienced significant treatment related mortality at the 25 degrees C test water, 45 degrees C aerial exposure temperature. Because of the high incidence of uprighting and survival of mussels in our study, emersion at moderate temperatures (15 to 35 degrees C) and durations (15 to 60 min) does not seem harmful to mussels, and, therefore, conducting relocations and status surveys under these conditions should not impair mussel survival and over-all success.

Journal of Shellfish Research↗
USGS1998

Oyster resource zones of the Barataria and Terrebonne estuaries of Louisiana

A 1:100,000 scale map delineating the subtidal oyster resource zones within the Barataria and Terrebonne estuaries was developed. Strategies to accomplish the task included interviews with Louisiana oystermen and state biologists to develop a draft map, field sampling to document oyster (Crassostrea virginica), Dermo (Perkinsus marinus), and oyster drill (Stramonita haemastoma) abundances, use of historical salinity data to aid in map verification, and public meetings to allow comment on a draft before final map preparation. Four oyster resource zones were delineated on the final map: a dry zone where subtidal oysters may be found when salinities increase, a wet zone where subtidal oysters may be found when salinities are suppressed, a wet-dry zone where subtidal oysters may be consistently found due to favorable salinities, and a high-salinity zone where natural oyster populations are predominantly found in intertidal and shallow waters. The dry zone is largely coincident with the brackish-marsh habitat, with some intermediate-type marsh. The wet-dry zone is found at the interface of the brackish and saline marshes, but extends further seaward than up-estuary. The wet zone and the high salinity zones are areas of mostly open water fringed by salt marshes. The dry zone encompasses 91,775 hectares, of which 48,788 hectares are water (53%). The wet zone encompasses 83,525 hectares, of which 66,958 hectares are water (80%). The wet-dry zone encompasses 171,893 hectares, of which 104,733 hectares are water (61%). The high salinity zone encompasses 125,705 hectares, of which 113,369 hectares are water (90%). There is a clear trend of increasing water habitat in the four zones over the past 30 years, and oysters are now cultivated on bottoms that were once marsh. The map should be useful in managing the effects upon oysters of freshwater diversions into the estuaries. It provides a pre-diversion record of the location of oyster resource zones and should prove helpful in the seaward relocation of oysters leases.

Journal of Shellfish Research↗
USGS2001

Seasonal variation in physiological condition of Amblema plicata in the Upper Mississippi River

Measures of physiological condition are being used as sub-lethal endpoints in studies with unionids exposed to a variety of stressors, yet the natural seasonal variation in these measures are largely undocumented. We measured concentrations of glycogen in foot and mantle tissue and a tissue condition index (TCI) in Amblema plicata (Say 1817), about monthly, for 2 years in mussels that were: (1) obtained directly from the Upper Mississippi River (riverine group); and (2) relocated from the river into an artificial pond (relocated group). In both groups, we observed significant seasonal variation in all physiological indicators. Seasonal variation in glycogen was 72% in mantle and 52% in foot tissue and paralleled reproductive activity in this short-term breeder. In the relocated group, most of the variation in glycogen occurred during the first six months after relocation, suggesting that handling stress may have been a contributing factor. The significant seasonal variation in the TCI paralleled glycogen in riverine mussels. We observed tissue-specific differences in glycogen in the riverine group, but not in the relocated group. These data suggest that an interaction of environmental and biological factors influence the energetic status of mussels in natural populations. A better understanding of this variation is needed to interpret changes in physiological condition due to stressors such as relocation.

Journal of Shellfish Research↗
USGS2001

Establishment of the green mussel, Perna viridis (Linnaeus 1758) (Mollusca: Mytilidae) on the West Coast of Florida

In 1999, the green mussel, Perna viridis, was first observed in Tampa Bay, Florida. This was the first reported occurrence of this Indo-Pacific marine bivalve in North America. The mussels found in Tampa Bay were confirmed to be P. viridis based on both morphological and genetic characteristics. Since the initial discovery, surveys in Tampa Bay and on the west coast of Florida have documented the growth, recruitment, and range expansion of P. viridis. From November 1999 to July 2000, the mean shell length of a Tampa Bay population increased from 49.0 mm to 94.1 mm, an increase of 97%. Populations of P. viridis are successfully reproducing in Tampa Bay. Recruitment was observed on sampling plates in May and continued through July 2000. The full extent of mussel colonization is not clear, but mussels were found outside Tampa Bay in St. Petersburg, Florida, south to Venice. Based on these studies it is evident that P. viridis has successfully invaded Tampa Bay and the west coast of Florida. The long-term impact of P. viridis on native communities off the west coast of Florida cannot be predicted at this time.

Journal of Shellfish Research↗