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At least 1,333 records · Page 74Linked to original sources
USGS2014

Bacterial pathogen gene abundance and relation to recreational water quality at seven Great Lakes beaches

Quantitative assessment of bacterial pathogens, their geographic variability, and distribution in various matrices at Great Lakes beaches are limited. Quantitative PCR (qPCR) was used to test for genes from E. coli O157:H7 ( eae O157 ), shiga-toxin producing E. coli ( stx2 ), Campylobacter jejuni ( mapA ), Shigella spp. ( ipaH ), and a Salmonella enterica -specific ( SE ) DNA sequence at seven Great Lakes beaches, in algae, water, and sediment. Overall, detection frequencies were mapA > stx2 > ipaH > SE > eae O157 . Results were highly variable among beaches and matrices; some correlations with environmental conditions were observed for mapA , stx2 , and ipaH detections. Beach seasonal mean mapA abundance in water was correlated with beach seasonal mean log 10 E. coli concentration. At one beach, stx2 gene abundance was positively correlated with concurrent daily E. coli concentrations. Concentration distributions for stx2 , ipaH , and mapA within algae, sediment, and water were statistically different (Non-Detect and Data Analysis in R). Assuming 10, 50, or 100% of gene copies represented viable and presumably infective cells, a quantitative microbial risk assessment tool developed by Michigan State University indicated a moderate probability of illness for Campylobacter jejuni at the study beaches, especially where recreational water quality criteria were exceeded. Pathogen gene quantification may be useful for beach water quality management.

Environmental Science & Technology↗
USGS2008

Environmental occurrence of the enterococcal surface protein ( esp ) gene is an unreliable indicator of human fecal contamination

The enterococcal surface protein ( esp ) gene found in Enterococcus faecalis and E. faecium has recently been explored as a marker of sewage pollution in recreational waters but its occurrence and distribution in environmental enterococci has not been well-documented. If the esp gene is found in environmental samples, there are potential implications for microbial source tracking applications. In the current study, a total of 452 samples (lake water, 100; stream water, 129; nearshore sand, 96; and backshore sand, 71; Cladophora sp. (Chlorophyta), 41; and periphyton (mostly Bacillariophyceae ), 15) collected from the coastal watersheds of southern Lake Michigan were selectively cultured for enterococci and then analyzed for the esp gene by PCR, targeting E. faecalis/ E. faecium ( esp fs/fm ) and E. faecium ( esp fm ). Overall relative frequencies for esp fs/fm and esp fm were 27.4 and 5.1%. Respective percent frequency for the esp fs/fm and esp fm was 36 and 14% in lake water; 38.8 and 2.3% in stream water; 24 and 6.3% in nearshore sand; 0% in backshore sand; 24.4 and 0% in Cladophora sp.; and 33.3 and 0% in periphyton. The overall occurrence of both esp fs/fm and esp fm was significantly related ( χ 2 = 49, P < 0.0001). Post-rain incidence of esp fs/fm increased in lake and stream water and nearshore sand. Further, E. coli and enterococci cell densities were significant predictors for esp fs/fm occurrence in post-rain lake water, but esp fm was not. F + coliphage densities were not significant predictors for esp fm or esp fs/fm gene incidence. In summary, the differential occurrence of the esp gene in the environment suggests that it is not limited to human fecal sources and thus may weaken its use as a reliable tool in discriminating contaminant sources (i.e., human vs nonhuman).

Environmental Science & Technology↗
USGS2010

Relationship and variation of qPCR and culturable enterococci estimates in ambient surface waters are predictable

The quantitative polymerase chain reaction (qPCR) method provides rapid estimates of fecal indicator bacteria densities that have been indicated to be useful in the assessment of water quality. Primarily because this method provides faster results than standard culture-based methods, the U.S. Environmental Protection Agency is currently considering its use as a basis for revised ambient water quality criteria. In anticipation of this possibility, we sought to examine the relationship between qPCR-based and culture-based estimates of enterococci in surface waters. Using data from several research groups, we compared enterococci estimates by the two methods in water samples collected from 37 sites across the United States. A consistent linear pattern in the relationship between cell equivalents (CCE), based on the qPCR method, and colony-forming units (CFU), based on the traditional culturable method, was significant (P < 0.05) at most sites. A linearly decreasing variance of CCE with increasing CFU levels was significant (P < 0.05) or evident for all sites. Both marine and freshwater sites under continuous influence of point-source contamination tended to reveal a relatively constant proportion of CCE to CFU. The consistency in the mean and variance patterns of CCE versus CFU indicates that the relationship of results based on these two methods is more predictable at high CFU levels (e.g., log 10 CFU > 2.0/100 mL) while uncertainty increases at lower CFU values. It was further noted that the relative error in replicated qPCR estimates was generally higher than that in replicated culture counts even at relatively high target levels, suggesting a greater need for replicated analyses in the qPCR method to reduce relative error. Further studies evaluating the relationship between culture and qPCR should take into account analytical uncertainty as well as potential differences in results of these methods that may arise from sample variability, different sources of pollution, and environmental factors.

Environmental Science & Technology↗
USGS2000

Potential for nonenzymatic reduction of Fe(III) via electron shuttling in subsurface sediments

The potential for various substances to serve as electron shuttles between Fe(III)-reducing microorganisms and insoluble Fe(III) oxides in aquifer sediments was evaluated in order to determine whether abiological mechanisms might play a role in the apparent microbial reduction of Fe(III) in subsurface sediments. Humic substances (humics) and the humics analogue, anthraquinone-2,6-disulfonate (AQDS), which were previously found to stimulate microbial reduction of synthetic poorly crystalline Fe(III) oxide under laboratory conditions, were found to also stimulate the reduction of aquifer Fe(III) oxides by indigenous microorganisms. Electron shuttling via biological reduction of U(VI) or S° followed by abiological reduction of Fe(III) by U(IV) or sulfide enhanced the reduction of synthetic Fe(III) oxide in cell suspensions, but these potential electron shuttles did not stimulate Fe(III) reduction when they were added to aquifer sediments. These results emphasize the importance of evaluating potential mechanisms for Fe(III) reduction with natural Fe(III) oxides, under environmentally relevant conditions. The finding that humics and other extracellular quinones can serve as electron shuttles to the Fe(III) oxides found in subsurface environments suggests that some Fe(III) reduction which was previously considered to be the result of direct enzymatic reduction of Fe(III) oxides may instead result from abiotic reduction of Fe(III) by microbially reduced humics or other microbially generated hydroquinones.

Environmental Science & Technology↗
USGS2002

Isolation and identification of trichothecenes from Fusarium compactum suspected in the aetiology of a major intoxication of sandhill cranes

Isoneosolaniol (4,8-diacetoxy-12,13-epoxytrichothec-9-ene-3,15-diol) and other unidentified trichothecene mycotoxins were isolated from culture extracts of two highly toxigenic strains of Fusarium compactum cultured from waste peanuts involved in an acute intoxication of sandhill cranes ( Grus canadensis ). Neosolaniol and other unidentified trichothecenes were detected in waste peanuts collected from affected areas. The structure of isoneosolaniol was determined by 1 H and 13 C NMR analyses and by high-resolution mass spectometry. Isoneosolaniol was hightly toxic to 1-day-old chickens and to a HEp2 cell culture assay. It was concluded that the most logical cause of the sandhill crane intoxication was Fusarium spp. Contaminated peanuts and various trichothecene mycotoxins acting alone or in conjunction with other Fusarium mycotoxins.

Texas↗
USGS2003

CO2 hydrate: Synthesis, composition, structure, dissociation behavior, and a comparison to structure I CH4 hydrate

Structure I (sI) carbon dioxide (CO 2 ) hydrate exhibits markedly different dissociation behavior from sI methane (CH 4 ) hydrate in experiments in which equilibrated samples at 0.1 MPa are heated isobarically at 13 K/h from 210 K through the H 2 O melting point (273.15 K). The CO 2 hydrate samples release only about 3% of their gas content up to temperatures of 240 K, which is 22 K above the hydrate phase boundary. Up to 20% is released by 270 K, and the remaining CO 2 is released at 271.0 plusmn; 0.5 K, where the sample temperature is buffered until hydrate dissociation ceases. This reproducible buffering temperature for the dissociation reaction CO 2 ·nH 2 O = CO 2 (g) + nH 2 O(1 to s) is measurably distinct from the pure H 2 O melting point at 273.15 K, which is reached as gas evolution ceases. In contrast, when si CH 4 hydrate is heated at the same rate at 0.1 MPa, >95% of the gas is released within 25 K of the equilibrium temperature (193 K at 0.1 MPa). In conjunction with the dissociation study, a method for efficient and reproducible synthesis of pure polycrystalline CO 2 hydrate with suitable characteristics for material properties testing was developed, and the material was characterized. CO 2 hydrate was synthesized from CO 2 liquid and H 2 O solid and liquid reactants at pressures between 5 and 25 MPa and temperatures between 250 and 281 K. Scanning electron microscopy (SEM) examination indicates that the samples consist of dense crystalline hydrate and 50-300 μm diameter pores that are lined with euhedral cubic hydrate crystals. Deuterated hydrate samples made by this same procedure were analyzed by neutron diffraction at temperatures between 4 and 215 K; results confirm that complete conversion of water to hydrate has occurred and that the measured unit cell parameter and thermal expansion are consistent with previously reported values. On the basis of measured weight gain after synthesis and gas yields from the dissociation experiments, approximately all cages in the hydrate structure are filled such that n ≈ 5.75.

Journal of Physical Chemistry B↗
USGS1997

Landscape characteristics of disturbed shrubsteppe habitats in southwestern Idaho (USA)

We compared 5 zones in shrubsteppe habitats of southwestern Idaho to determine the effect of differing disturbance combinations on landscapes that once shared historically similar disturbance regimes. The primary consequence of agriculture, wildfires, and extensive fires ignited by the military during training activities was loss of native shrubs from the landscape. Agriculture created large square blocks on the landscape, and the landscape contained fewer small patches and more large shrub patches than non-agricultural areas. In contrast, fires left a more fragmented landscape. Repeated fires did not change the distribution of patch sizes, but decreased the total area of remaining shrublands and increased the distance between remaining shrub patches that provide seed sources. Military training with tracked vehicles was associated with a landscape characterized by small, closely spaced, shrub patches. Our results support the general model hypothesized for conversion of shrublands to annual grasslands by disturbance. Larger shrub patches in our region, historically resistant to fire spread and large-scale fires because of a perennial bunchgrass understory, were more fragmented than small patches. Presence of cheatgrass (Bromus tectorum), an exotic annual, was positively related to landscape patchiness and negatively related to number of shrub cells. Thus, cheatgrass dominance can contribute to further fragmentation and loss of the shrub patch by facilitating spread of subsequent fires, carried by continuous fuels, through the patch. The synergistic processes of fragmentation of shrub patches by disturbance, invasion and subsequent dominance by exotic annuals, and fire are converting shrubsteppe in southwestern Idaho to a new state dominated by exotic annual grasslands and high fire frequencies.

Idaho↗
USGS1998

Flow-cytometric determination of genotoxic effects of exposure to petroleum in mink and sea otters

Three experiments were conducted to investigate the genotoxic effects of crude oil on mink and sea otters, In the first experiment, the effects on mink of chronic exposure to weathered Prudhoe Bay crude oil were studied, Female mink were fed a diet that included weathered crude oil for a period of 3 weeks prior to mating, during pregnancy and until weaning. Kits were exposed through lactation and by diet after weaning until 4 months of age. Kidney and liver tissues of the kits were examined using flow cytometry (FCM) and it was found that the genome size was increased in kidney samples from the experimental group compared to the control group. This effect was probably due to some type of DNA amplification and it could have been inherited from the exposed mothers or have been a somatic response to oil exposure in the pups, No evidence of clastogenic effects, as measured by the coefficient of variation (CV) of the G(1) peak, was found in kidney or liver tissue. In the second experiment, yearling female mink were exposed either by diet or externally to crude oil or bunker C fuel oil. Evidence for clastogenic damage was found in spleen tissue for the exposure groups, but not in kidney tissue. No evidence of increased genome size was observed. In the third experiment, blood was obtained from wild-caught sea otters in Prince William Sound. The sea otters represented two populations: one from western Prince William Sound that was potentially exposed to oil from the Exxon Valdez oil spill and a reference population from eastern Prince William Sound that did not receive oil from the spill. The spill had occurred 1.5 years prior to obtaining the blood samples. Although the mean CVs did not differ between the populations, the exposed population had a significantly higher variance of CV measurements and five out of 15 animals from the exposed population had CVs higher than the 95% confidence limits of the reference population, It is concluded that FCM is a sensitive indicator of the clastogenic effects of oil exposure and that haematopoietic tissues and blood are best for detecting clastogenic damage. Moreover, the observed differences in the genome size of the kidney cells mere possibly heritable effects, but this needs further investigation. Lastly, sea otters exposed to spilled oil 1.5 years earlier showed evidence of clastogenic damage in one-third of the individuals sampled.

Ecotoxicology↗
USGS2001

Representation of natural vegetation in protected areas: Capturing the geographic range

Current conservation strategies for plant and animal species rarely address the need to protect the species throughout its geographic range thereby capturing potential genetic and ecological variation. We examined the degree that existing protected areas in the western United States satisfied this goal for four widespread vegetation cover types. We used latitude and longitude to stratify the distribution of these types into 16 cells, each of which was further stratified by up to five elevation classes. While protection of some vegetation types was high in parts of their range, it was minimal to nonexistent in other parts. While it is yet to be shown that protecting a given species throughout its geographic range is essential for its long-term existence, in the face of often unpredictable environmental changes, it seems a prudent course to follow. Our results suggest that if full range protection is a conservation goal, the existing network of protected areas may be inadequate for the task.

Biodiversity and Conservation↗
USGS2001

Cytochrome b sequences in black-crowned night-herons (Nycticorax nycticorax) from heronries exposed to genotoxic contaminants

DNA sequence analysis of a 215 base-pair region of the mitochondrial cytochrome b gene was used to examine genetic variation and search for evidence of an increased mutation rate in black-crowned night-herons. We examined five populations exposed to environmental contamination (primarily PAHs and PCBs) and one reference population from the eastern U.S. There was no evidence of a high mutation rate even within populations previously shown to exhibit increased variation in DNA content among somatic cells as a result of petroleum exposure. Three haplotypes were observed among 99 individuals. The low level of variability could be evidence for a genetic bottleneck, or that cytochrome b is too conservative for use in population genetic studies of this species. With the exception of one population from Louisiana, pair-wise Phi st estimates were very low, indicative of little population structure and potentially high rates of effective migration among populations.

Ecotoxicology↗
USGS2002

Preliminary cellular-automata forecast of permit activity from 1998 to 2010, Idaho and Western Montana

For public land management in Idaho and western Montana, the U.S. Forest Service (USFS) has requested that the U.S. Geological Survey (USGS) predict where mineral-related activity will occur in the next decade. Cellular automata provide an approach to simulation of this human activity. Cellular automata (CA) are defined by an array of cells , which evolve by a simple transition rule, the automaton . Based on exploration trends, we assume that future exploration will focus in areas of past exploration. Spatial-temporal information about mineral-related activity, that is permits issued by USFS and Bureau of Land Management (BLM) in the last decade, and spatial information about undiscovered resources, provide a basis to calibrate a CA. The CA implemented is a modified annealed voting rule that simulates mineral-related activity with spatial and temporal resolution of 1 mi 2 and 1 year based on activity from 1989 to 1998. For this CA, the state of the economy and exploration technology is assumed constant for the next decade. The calibrated CA reproduces the 1989–1998-permit activity with an agreement of 94%, which increases to 98% within one year. Analysis of the confusion matrix and kappa correlation statistics indicates that the CA underestimates high activity and overestimates low activity. Spatially, the major differences between the actual and calculated activity are that the calculated activity occurs in a slightly larger number of small patches and is slightly more uneven than the actual activity. Using the calibrated CA in a Monte Carlo simulation projecting from 1998 to 2010, an estimate of the probability of mineral activity shows high levels of activity in Boise, Caribou, Elmore, Lincoln, and western Valley counties in Idaho and Beaverhead, Madison, and Stillwater counties in Montana, and generally low activity elsewhere.

Idaho, Montana↗
USGS2004

Terrestrial microorganisms at an altitude of 20,000 m in Earth's atmosphere

A joint effort between the U.S. Geological Survey's (USGS) Global Desert Dust and NASA's Stratospheric and Cosmic Dust Programs identified culturable microbes from an air sample collected at an altitude of 20,000 m. A total of 4 fungal (Penicillium sp.) and 71 bacteria colonyforming units (70 colonies of Bacillus luciferensis believed to have originated from a single cell collected at altitude and one colony of Bacillus sphaericus) were enumerated, isolated and identified using a morphological key and 16S rDNA sequencing respectively. All of the isolates identified were sporeforming pigmented fungi or bacteria of terrestrial origin and demonstrate that the presence of viable microorganisms in Earth's upper atmosphere may not be uncommon.

Aerobiologia↗
USGS2004

Physiological changes in largemouth bass exposed to paper mill effluents under laboratory and field conditions

We report here on studies designed to asses the effects of paper mill effluents on non-reproductive functions of free-ranging and captive Florida largemouth bass (Micropterus salmoides floridanus) This was accomplished by conducting an outdoor tank study, in which fish were exposed to well water or to 10%, 20%, 40%, and 80% full strength effluent for 28 or 56 days, and by sampling largemouth bass from sites within the St. Johns River, Florida, upstream and downstream from a paper mill plant. Blood and plasma samples from fish from the tank study and from fish sampled from the ambient sites were analyzed for over 20 variables. We also determined liver and spleen weights and examined them histologically. The most significant finding from the tank study was an increase in the concentration of albumin and hepatosomatic index for bass exposed to ???20% effluents for 56 days. Spleenosomatic index and number of melanomacrophage centers were decreased in bass from effluent-dominated sites (Palatka and Rice Creek), whereas concentrations of calcium, phosphorous, glucose, and creatinine were elevated in fish from these sites, compared to fish from reference streams. Fish from Rice Creek also had fewer red blood cells, and male bass from Palatka had lower concentrations of cholesterol. Plasma concentrations of albumin and hepatic concentrations of glutathione were elevated in males from Palatka, and both females and males from Rice Creek had higher concentrations of globulin. These results indicate a complex pattern of effects of paper mill effluents on several physiological functions. However, despite the myriad of treatment and site-related effects, most physiological parameters fell within normal ranges when compared to reports on largemouth bass and other freshwater species.

Ecotoxicology↗
USGS2001

Statistical self-similarity of hotspot seamount volumes modeled as self-similar criticality

The processes responsible for hotspot seamount formation are complex, yet the cumulative frequency-volume distribution of hotspot seamounts in the Easter Island/Salas y Gomez Chain (ESC) is found to be well-described by an upper-truncated power law. We develop a model for hotspot seamount formation where uniform energy input produces events initiated on a self-similar distribution of critical cells. We call this model Self-Similar Criticality (SSC). By allowing the spatial distribution of magma migration to be self-similar, the SSC model recreates the observed ESC seamount volume distribution. The SSC model may have broad applicability to other natural systems.

Geophysical Research Letters↗
USGS2004

Granular avalanches across irregular three-dimensional terrain: 1. Theory and computation

To establish a theoretical basis for predicting and interpreting the behavior of rapid mass movements on Earth's surface, we develop and test a new computational model for gravity-driven motion of granular avalanches across irregular, three-dimensional (3-D) terrain. The principles embodied in the model are simple and few: continuum mass and momentum conservation and intergranular stress generation governed by Coulomb friction. However, significant challenges result from the necessity of satisfying these principles when deforming avalanches interact with steep and highly variable 3-D terrain. We address these challenges in four ways. (1) We formulate depth-averaged governing equations that are referenced to a rectangular Cartesian coordinate system (with z vertical) and that account explicitly for the effect of nonzero vertical accelerations on depth-averaged mass and momentum fluxes and stress states. (2) We compute fluxes of mass and momentum across vertical cell boundaries using a high-resolution finite volume method and Roe-type Riemann solver. Our algorithm incorporates flux difference splitting, an entropy correction for the flux, and eigenvector decomposition to embed the effects of driving and resisting forces in Riemann solutions. (3) We use a finite element method and avalanche displacements predicted by Riemann solutions to compute Coulomb stresses conjugate to the displacements in 3-D stress space. (4) We test the model output against analytical solutions, a sand cone conceptual experiment, and (in a companion paper) data from detailed laboratory experiments. Model results illustrate a complex interplay of basal traction and internal stress, and they successfully predict not only the gross behavior but also many details of avalanche motion from initiation to deposition.

Journal of Geophysical Research Earth Surface↗
USGS2005

Response of seismicity to Coulomb stress triggers and shadows of the 1999 Mw=7.6 Chi-Chi, Taiwan, earthquake

The correlation between static Coulomb stress increases and aftershocks has thus far provided the strongest evidence that stress changes promote seismicity, a correlation that the Chi-Chi earthquake well exhibits. Several studies have deepened the argument by resolving stress changes on aftershock focal mechanisms, which removes the assumption that the aftershocks are optimally oriented for failure. Here one compares the percentage of planes on which failure is promoted after the main shock relative to the percentage beforehand. For Chi-Chi we find a 28% increase for thrust and an 18% increase for strike-slip mechanisms, commensurate with increases reported for other large main shocks. However, perhaps the chief criticism of static stress triggering is the difficulty in observing predicted seismicity rate decreases in the stress shadows, or sites of Coulomb stress decrease. Detection of sustained drops in seismicity rate demands a long catalog with a low magnitude of completeness and a high seismicity rate, conditions that are met at Chi-Chi. We find four lobes with statistically significant seismicity rate declines of 40-90% for 50 months, and they coincide with the stress shadows calculated for strike-slip faults, the dominant faulting mechanism. The rate drops are evident in uniform cell calculations, 100-month time series, and by visual inspection of the M ??? 3 seismicity. An additional reason why detection of such declines has proven so rare emerges from this study: there is a widespread increase in seismicity rate during the first 3 months after Chi-Chi, and perhaps many other main shocks, that might be associated with a different mechanism. Copyright 2005 by the American Geophysical Union.

Journal of Geophysical Research B: Solid Earth↗
USGS2005

Forecasting the evolution of seismicity in southern California: Animations built on earthquake stress transfer

We develop a forecast model to reproduce the distibution of main shocks, aftershocks and surrounding seismicity observed during 1986-200 in a 300 ?? 310 km area centered on the 1992 M = 7.3 Landers earthquake. To parse the catalog into frames with equal numbers of aftershocks, we animate seismicity in log time increments that lengthen after each main shock; this reveals aftershock zone migration, expansion, and densification. We implement a rate/state algorithm that incorporates the static stress transferred by each M ??? 6 shock and then evolves. Coulomb stress changes amplify the background seismicity, so small stress changes produce large changes in seismicity rate in areas of high background seismicity. Similarly, seismicity rate declines in the stress shadows are evident only in areas with previously high seismicity rates. Thus a key constituent of the model is the background seismicity rate, which we smooth from 1981 to 1986 seismicity. The mean correlation coefficient between observed and predicted M ??? 1.4 shocks (the minimum magnitude of completeness) is 0.52 for 1986-2003 and 0.63 for 1992-2003; a control standard aftershock model yields 0.54 and 0.52 for the same periods. Four M ??? 6.0 shocks struck during the test period; three are located at sites where the expected seismicity rate falls above the 92 percentile, and one is located above the 75 percentile. The model thus reproduces much, but certainly not all, of the observed spatial and temporal seismicity, from which we infer that the decaying effect of stress transferred by successive main shocks influences seismicity for decades. Finally, we offer a M ??? 5 earthquake forecast for 2005-2015, assigning probabilities to 324 10 ?? 10 km cells.

Journal of Geophysical Research B: Solid Earth↗
USGS2006

Role of microbial iron reduction in the dissolution of iron hydroxysulfate minerals

Iron-hydroxysulfate minerals can be important hosts for metals such as lead, mercury, copper, zinc, silver, chromium, arsenic, and selenium and for radionuclides such as 226Ra. These mineral-bound contaminants are considered immobilized under oxic conditions. However, when anoxic conditions develop, the activities of sulfate- or iron-reducing bacteria could result in mineral dissolution, releasing these bound contaminants. Reduction of structural sulfate in the iron-hydroxysulfate mineral jarosite by sulfate-reducing bacteria has previously been demonstrated. The primary objective of this work was to evaluate the potential for anaerobic dissolution of the iron-hydroxysulfate minerals jarosite and schwertmannite at neutral PH by iron-reducing bacteria. Mineral dissolution was tested using a long-term cultivar, Geobacter metallireducens strain GS-15, and a fresh isolate Geobacter sp. strain ENN1, previously undescribed. ENN1 was isolated from the discharge site of Shadle Mine, in the southern anthracite coalfield of Pennsylvania, where schwertmannite was the predominant iron-hydroxysulfate mineral. When jarosite from Elizabeth Mine (Vermont) was provided as the sole terminal electron acceptor, resting cells of both G. metallireducens and ENN1 were able to reduce structural Fe(III), releasing Fe+2, SO4-2, and K+ ions. A lithified jarosite sample from Utah was more resistant to microbial attack, but slow release of Fe+2 was observed. Neither bacterium released Fe+2 from poorly crystalline synthetic schwertmannite. Our results indicate that exposure of jarosite to iron-reducing conditions at neutral pH is likely to promote the mobility of hazardous constituents and should therefore be considered in evaluating waste disposal and/or reclamation options involving jarosite-bearing materials.

Journal of Geophysical Research G: Biogeosciences↗