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Assessing the influence of watershed characteristics on chlorophyll a in waterbodies at global and regional scales

Prediction of primary production of lentic water bodies (i.e., lakes and reservoirs) is valuable to researchers and resource managers alike, but is very rarely done at the global scale. With the development of remote sensing technologies, it is now feasible to gather large amounts of data across the world, including understudied and remote regions. To determine which factors were most important in explaining the variation of chlorophyll a (Chl- a ), an indicator of primary production in water bodies, at global and regional scales, we first developed a geospatial database of 227 water bodies and watersheds with corresponding Chl- a , nutrient, hydrogeomorphic, and climate data. Then we used a generalized additive modeling approach and developed model selection criteria to select models that most parsimoniously related Chl- a to predictor variables for all 227 water bodies and for 51 lakes in the Laurentian Great Lakes region in the data set. Our best global model contained two hydrogeomorphic variables (water body surface area and the ratio of watershed to water body surface area) and a climate variable (average temperature in the warmest model selection criteria to select models that most parsimoniously related Chl- a to predictor variables quarter) and explained ~ 30% of variation in Chl- a . Our regional model contained one hydrogeomorphic variable (flow accumulation) and the same climate variable, but explained substantially more variation (58%). Our results indicate that a regional approach to watershed modeling may be more informative to predicting Chl- a , and that nearly a third of global variability in Chl- a may be explained using hydrogeomorphic and climate variables.

Inland Waters↗

Acid mine drainage

Acid mine drainage (AMD) consists of metal-laden solutions produced by the oxidative dissolution of iron sulfide minerals exposed to air, moisture, and acidophilic microbes during the mining of coal and metal deposits. The pH of AMD is usually in the range of 2–6, but mine-impacted waters at circumneutral pH (5–8) are also common. Mine drainage usually contains elevated concentrations of sulfate, iron, aluminum, and other potentially toxic metals leached from rock that hydrolyze and coprecipitate to form rust-colored encrustations or sediments. When AMD is discharged into surface waters or groundwaters, degradation of water quality, injury to aquatic life, and corrosion or encrustation of engineered structures can occur for substantial distances. Prevention and remediation strategies should consider the biogeochemical complexity of the system, the longevity of AMD pollution, the predictive power of geochemical modeling, and the full range of available field technologies for problem mitigation.

Book chapter↗

Assessing macroinvertebrate biodiversity in freshwater ecosystems: Advances and challenges in dna-based approaches

Assessing the biodiversity of macroinvertebrate fauna in freshwater ecosystems is an essential component of both basic ecological inquiry and applied ecological assessments. Aspects of taxonomic diversity and composition in freshwater communities are widely used to quantify water quality and measure the efficacy of remediation and restoration efforts. The accuracy and precision of biodiversity assessments based on standard morphological identifications are often limited by taxonomic resolution and sample size. Morphologically based identifications are laborious and costly, significantly constraining the sample sizes that can be processed. We suggest that the development of an assay platform based on DNA signatures will increase the precision and ease of quantifying biodiversity in freshwater ecosystems. Advances in this area will be particularly relevant for benthic and planktonic invertebrates, which are often monitored by regulatory agencies. Adopting a genetic assessment platform will alleviate some of the current limitations to biodiversity assessment strategies. We discuss the benefits and challenges associated with DNA-based assessments and the methods that are currently available. As recent advances in microarray and next-generation sequencing technologies will facilitate a transition to DNA-based assessment approaches, future research efforts should focus on methods for data collection, assay platform development, establishing linkages between DNA signatures and well-resolved taxonomies, and bioinformatics. ?? 2010 by The University of Chicago Press.

The Quarterly Review of Biology↗

Principles for urban stormwater management to protect stream ecosystems

Urban stormwater runoff is a critical source of degradation to stream ecosystems globally. Despite broad appreciation by stream ecologists of negative effects of stormwater runoff, stormwater management objectives still typically center on flood and pollution mitigation without an explicit focus on altered hydrology. Resulting management approaches are unlikely to protect the ecological structure and function of streams adequately. We present critical elements of stormwater management necessary for protecting stream ecosystems through 5 principles intended to be broadly applicable to all urban landscapes that drain to a receiving stream: 1) the ecosystems to be protected and a target ecological state should be explicitly identified; 2) the postdevelopment balance of evapotranspiration, stream flow, and infiltration should mimic the predevelopment balance, which typically requires keeping significant runoff volume from reaching the stream; 3) stormwater control measures (SCMs) should deliver flow regimes that mimic the predevelopment regime in quality and quantity; 4) SCMs should have capacity to store rain events for all storms that would not have produced widespread surface runoff in a predevelopment state, thereby avoiding increased frequency of disturbance to biota; and 5) SCMs should be applied to all impervious surfaces in the catchment of the target stream. These principles present a range of technical and social challenges. Existing infrastructural, institutional, or governance contexts often prevent application of the principles to the degree necessary to achieve effective protection or restoration, but significant potential exists for multiple co-benefits from SCM technologies (e.g., water supply and climate-change adaptation) that may remove barriers to implementation. Our set of ideal principles for stream protection is intended as a guide for innovators who seek to develop new approaches to stormwater management rather than accept seemingly insurmountable historical constraints, which guarantee future, ongoing degradation.

Freshwater Science↗

Using environmental DNA (eDNA) to detect the endangered Spectaclecase Mussel ( Margaritifera monodonta )

Margaritifera monodonta , or the Spectaclecase Mussel, is a federally endangered freshwater mussel species that has experienced a 55% reduction in range and is currently concentrated in 3 rivers in the Midwest region of the United States (Gasconade and Meramec rivers, Missouri, and St Croix River, Wisconsin). The detection of new populations by traditional survey methods has been limited because these mussels tend to occur under large rocks and boulders. Environmental DNA (eDNA) technology has been used to detect invasive and rare species, but its use for detection of rare, benthic-dwelling species in large flowing systems has been limited. Here, we propose using eDNA to assess known populations of M. monodonta . We designed a M. monodonta -specific quantitative polymerase chain reaction (qPCR) assay and tested it using water samples from multiple M. monodonta housing tanks, water samples from 2 known mussel beds on the St Croix River, and water samples from 3 known mussel beds on the Mississippi River. We observed higher overall eDNA detection rates on the St Croix River (30.2%) compared to the upper Mississippi River (0.60%). We also observed higher eDNA detection rates (73.3–93.1%) in 2018 for samples collected during the larval release period in May compared to samples collected in August after the reproductive period had ended (55.6–70.8%) on the St Croix River. We tested samples collected at 3 distances downstream from the 2 mussel beds found in the St Croix River, but we did not observe a substantial effect of distance on our detection rates. However, we did observe greater detection rates for samples collected near the bottom compared to at the surface. Our results indicate that this novel qPCR assay can successfully detect M. monodonta eDNA and could be used to rapidly screen locations to guide intensive physical searches for populations in riverine systems.

Wisconsin, Missouri↗

Vulnerabilities and opportunities at the nexus of electricity, water and climate

The articles in this special issue examine the critical nexus of electricity, water, and climate, emphasizing connections among resources; the prospect of increasing vulnerabilities of water resources and electricity generation in a changing climate; and the opportunities for research to inform integrated energy and water policy and management measures aimed at reducing vulnerability and increasing resilience. Here, we characterize several major themes emerging from this research and highlight some of the uptake of this work in both scientific and public spheres. Underpinning much of this research is the recognition that water resources are expected to undergo substantial changes based on the global warming that results primarily from fossil energy-based carbon emissions. At the same time, the production of electricity from fossil fuels, nuclear power, and some renewable technologies (biomass, geothermal and concentrating solar power) can be highly water-intensive. Energy choices now and in the near future will have a major impact not just on the global climate, but also on water supplies and the resilience of energy systems that currently depend heavily on them.

Environmental Research Letters↗

Using constructed analogs to improve the skill of National Multi-Model Ensemble March–April–May precipitation forecasts in equatorial East Africa

In this study we implement and evaluate a simple 'hybrid' forecast approach that uses constructed analogs (CA) to improve the National Multi-Model Ensemble's (NMME) March–April–May (MAM) precipitation forecasts over equatorial eastern Africa (hereafter referred to as EA, 2°S to 8°N and 36°E to 46°E). Due to recent declines in MAM rainfall, increases in population, land degradation, and limited technological advances, this region has become a recent epicenter of food insecurity. Timely and skillful precipitation forecasts for EA could help decision makers better manage their limited resources, mitigate socio-economic losses, and potentially save human lives. The 'hybrid approach' described in this study uses the CA method to translate dynamical precipitation and sea surface temperature (SST) forecasts over the Indian and Pacific Oceans (specifically 30°S to 30°N and 30°E to 270°E) into terrestrial MAM precipitation forecasts over the EA region. In doing so, this approach benefits from the post-1999 teleconnection that exists between precipitation and SSTs over the Indian and tropical Pacific Oceans (Indo-Pacific) and EA MAM rainfall. The coupled atmosphere-ocean dynamical forecasts used in this study were drawn from the NMME. We demonstrate that while the MAM precipitation forecasts (initialized in February) skill of the NMME models over the EA region itself is negligible, the ranked probability skill score of hybrid CA forecasts based on Indo-Pacific NMME precipitation and SST forecasts reach up to 0.45.

Environmental Research Letters↗

Wind, sun, and wildlife: Do wind and solar energy development “short-circuit” conservation in the western United States?

Despite the trade-offs between renewable energy development, land use, humans, and wildlife, wind and solar development continues to transform the southwestern US into a green energy landscape. While renewable energy reduces carbon emissions and reliance on fossil fuels, many studies have emerged on the associated ecological and social impacts of this technology. Here, we review the current state of knowledge on the nexus between wildlife conservation and energy development in the western US since 2010. We revisit pertinent ecological concepts and questions presented in earlier reviews to assess how far the field has progressed in mitigating negative effects. Specifically, we ask, what density or design of development maximizes energy benefits while minimizing negative effects on wildlife, whether the results of previously-deficient before-after control-impact studies are now more readily-available, and ultimately, can the impacts of renewable energy development on wildlife be mitigated? We also provide a case study on the federally-protected Desert Tortoise, a conservation-reliant species in the Desert Southwest US, to highlight efforts to mitigate the negative effects of renewable energy development. Our review concludes that successful mitigation is possible via use of spatial decision support tools, applying novel wildlife deterrence and detection systems developed for existing installed facilities, and incorporating impact studies that provide managers with conservation metrics for evaluating different future development land-use scenarios.

Environmental Research Letters↗

Early detection of wildlife disease pathogens using CRISPR-Cas system methods

Wildlife diseases are a considerable threat to human health, conservation, and the economy. Surveillance is a critical component to mitigate the impact of animal diseases in these sectors. To monitor human diseases, CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR-associated protein) biosensors have proven instrumental as diagnostic tools capable of detecting unique DNA and RNA sequences related to their associated pathogens. However, despite the significant advances in the general development of CRISPR-Cas biosensors, their use to support wildlife disease management is lagging. In some cases, wildlife diseases of concern could be rapidly surveyed using these tools with minimal technical, operational, or cost requirements to end users. This review explores the potential to further leverage this technology to advance wildlife disease monitoring and highlights how concerted standardization of protocols can help to ensure data reliability.

The CRISPR Journal↗

Characterization of Lone Pine, California, tremolite asbestos and preparation of research material

Well-characterized amphibole asbestos mineral samples are required for use as analytical standards and in future research projects. Currently, the National Institute for Standards and Technology Standard Reference Material samples of asbestos are listed as ‘Discontinued’. The National Institute for Occupational Safety and Health (NIOSH) has a goal under the Asbestos Roadmap of locating and characterizing research materials for future use. Where an initial characterization analysis determines that a collected material is appropriate for use as a research material in terms of composition and asbestiform habit, sufficient amounts of the material will be collected to make it publicly available. An abandoned mine near Lone Pine, California, contains a vein of tremolite asbestos, which was the probable source of a reference material that has been available for the past 17 years from the Health and Safety Laboratory (HSL) in the UK. Newly collected fibrous vein material from this mine was analyzed at Research Triangle Institute (RTI International) with some additional analysis by the US Geological Survey’s Denver Microbeam Laboratory. The analysis at RTI International included: (i) polarized light microscopy (PLM) with a determination of principal optical properties; (ii) X-ray diffraction; (iii) transmission electron microscopy, including energy dispersive X-ray spectroscopy and selected-area electron diffraction; and (iv) spindle stage analysis using PLM to determine whether individual fibers and bundles of the samples were polycrystalline or single-crystal cleavage fragments. The overall findings of the study indicated that the material is tremolite asbestos with characteristics substantially similar to the earlier distributed HSL reference material. A larger quantity of material was prepared by sorting, acid-washing and mixing for sub-division into vials of ~10g each. These vials have been transferred from NIOSH to RTI International, from where they can be obtained on request.

California↗

Global commitments to conserving and monitoring genetic diversity are now necessary and feasible

Global conservation policy and action have largely neglected protecting and monitoring genetic diversity—one of the three main pillars of biodiversity. Genetic diversity (diversity within species) underlies species’ adaptation and survival, ecosystem resilience, and societal innovation. The low priority given to genetic diversity has largely been due to knowledge gaps in key areas, including the importance of genetic diversity and the trends in genetic diversity change; the perceived high expense and low availability and the scattered nature of genetic data; and complicated concepts and information that are inaccessible to policymakers. However, numerous recent advances in knowledge, technology, databases, practice, and capacity have now set the stage for better integration of genetic diversity in policy instruments and conservation efforts. We review these developments and explore how they can support improved consideration of genetic diversity in global conservation policy commitments and enable countries to monitor, report on, and take action to maintain or restore genetic diversity.

BioScience↗

Future of coral bleaching research

Coral bleaching is the largest global threat to coral reef ecosystem persistence this century. Advancing our understanding of coral bleaching and developing solutions to protect corals and the reefs they support are critical. In the present article, we, the US National Science Foundation–funded Coral Bleaching Research Coordination Network, outline future directions for coral bleaching research. Specifically, we address the need for embedded inclusiveness, codevelopment, and capacity building as a foundation for excellence in coral bleaching research and the critical role of coral-bleaching science in shaping policy. We outline a path for research innovation and technology and propose the formation of an international coral bleaching consortium that, in coordination with existing multinational organizations, could be a hub for planning, coordinating, and integrating global-scale coral bleaching research, innovation, and mitigation strategies. This proposed strategy for future coral bleaching research could facilitate a step-function change in how we address the coral bleaching crisis.

BioScience↗

The role of fire on Earth

Fire is a defining feature of our biosphere, having appeared when the first plants colonized the land, and it continues to occur across the planet at different frequencies and intensities. Fire has been and remains as an evolutionary force in many plant and animal lineages and contributes to explaining the variability of our biodiversity. Fire has also shaped the structure of many ecosystems and the distribution of biomes, and it is an important contributor to the global biogeochemical cycles. In addition, fire has been a key factor in human evolution, and, in turn, humans have modified fire regimes with important consequences for the biosphere. Consequently, fire is an intrinsic factor on our planet. Our challenge now is to understand and predict the role of fire in a densely populated, highly technological world that imposes significant changes on the Earth.

BioScience↗

Now hiring! Empirically testing a three-step intervention to increase faculty gender diversity in STEM

Workforce homogeneity limits creativity, discovery, and job satisfaction; nonetheless, the vast majority of university faculty in science, technology, engineering, and mathematics (STEM) fields are men. We conducted a randomized and controlled three-step faculty search intervention based in self-determination theory aimed at increasing the number of women faculty in STEM at one US university where increasing diversity had historically proved elusive. Results show that the numbers of women candidates considered for and offered tenure-track positions were significantly higher in the intervention groups compared with those in controls. Searches in the intervention were 6.3 times more likely to make an offer to a woman candidate, and women who were made an offer were 5.8 times more likely to accept the offer from an intervention search. Although the focus was on increasing women faculty within STEM, the intervention can be adapted to other scientific and academic communities to advance diversity along any dimension.

BioScience↗

Global positioning system tracking devices can decrease Greater Sage-Grouse survival

Reliable demographic estimates hinge on the assumption that marking animals does not alter their behavior, reproduction, or survival. Violations can bias inference and are especially egregious for species of high conservation concern. Global positioning system (GPS) devices represent a recent technological advancement that has contributed greatly to avian ecological studies compared with traditionally used very high frequency (VHF) radio transmitters, but may affect demographic rates differently than VHF transmitters. We compared survival between VHF (necklace attachment) and GPS (rump-mounted attachment) devices from >1,100 Greater Sage-Grouse ( Centrocercus urophasianus ), a species of high conservation concern, across multiple populations within California and Nevada. We found lower survival for GPS-marked compared to VHF-marked sage-grouse across most sex, age, and seasonal comparisons. Estimates of annual survival for GPS-marked sage-grouse were 0.55–0.86 times that of VHF-marked birds with considerable variation among sex and age classes. Differences in survival could be attributed to features associated with GPS devices, including greater weight, position of attachment (e.g., rump-mount harness), and a semi-reflective solar panel. In a post hoc analysis, we evaluated additive and interactive effects between device type (GPS vs. VHF) and transmitter mass as a proportion of body mass (PBM). While the device type effect alone was the best model, the PBM interaction also had support. For GPS devices, survival decreased with increasing PBM, whereas PBM effects were not found for VHF. We attributed differences in PBM effect to placement of transmitters on sage-grouse, as weight of GPS devices was positioned rearward. This information can help managers and researchers weigh costs and benefits of GPS-based monitoring. Our results indicate demographic data collected from GPS devices should be interpreted with caution, and use of these devices should be tailored to specific ecological questions. Future research aimed at investigating behavioral impacts and GPS designs that reduce adverse impacts on survival would be beneficial.

California, Nevada↗

Selecting auditory alerting stimuli for eagles on the basis of auditory evoked potentials

Development of wind energy facilities results in interactions between wildlife and wind turbines. Raptors, including bald and golden eagles, are among the species known to incur mortality from these interactions. Several alerting technologies have been proposed to mitigate this mortality by increasing eagle avoidance of wind energy facilities. However, there has been little attempt to match signals used as alerting stimuli with the sensory capabilities of target species like eagles. One potential approach to tuning signals is to use sensory physiology to determine what stimuli the target eagle species are sensitive to even in the presence of background noise, thereby allowing the development of a maximally stimulating signal. To this end, we measured auditory evoked potentials of bald and golden eagles to determine what types of sounds eagles can process well, especially in noisy conditions. We found that golden eagles are significantly worse than bald eagles at processing rapid frequency changes in sounds, but also that noise effects on hearing in both species are minimal in response to rapidly changing sounds. Our findings therefore suggest that sounds of intermediate complexity may be ideal both for targeting bald and golden eagle hearing and for ensuring high stimulation in noisy field conditions. These results suggest that the sensory physiology of target species is likely an important consideration when selecting auditory alerting sounds and may provide important insight into what sounds have a reasonable probability of success in field applications under variable conditions and background noise.

Conservation Physiology↗

MODIS imagery improves pest risk assessment: A case study of wheat stem sawfly (Cephus cinctus, Hymenoptera: Cephidae) in Colorado, USA

Wheat stem sawfly ( Cephus cinctus Norton, Hymenoptera: Cephidae) has long been a significant insect pest of spring, and more recently, winter wheat in the northern Great Plains. Wheat stem sawfly was first observed infesting winter wheat in Colorado in 2010 and, subsequently, has spread rapidly throughout wheat production regions of the state. Here, we used maximum entropy modeling (MaxEnt) to generate habitat suitability maps in order to predict the risk of crop damage as this species spreads throughout the winter wheat-growing regions of Colorado. We identified environmental variables that influence the current distribution of wheat stem sawfly in the state and evaluated whether remotely sensed variables improved model performance. We used presence localities of C. cinctus and climatic, topographic, soils, and normalized difference vegetation index and enhanced vegetation index data derived from Moderate Resolution Imaging Spectroradiometer (MODIS) imagery as environmental variables. All models had high performance in that they were successful in predicting suitable habitat for C. cinctus in its current distribution in eastern Colorado. The enhanced vegetation index for the month of April improved model performance and was identified as a top contributor to MaxEnt model. Soil clay percent at 0–5 cm, temperature seasonality, and precipitation seasonality were also associated with C. cinctus distribution in Colorado. The improved model performance resulting from integrating vegetation indices in our study demonstrates the ability of remote sensing technologies to enhance species distribution modeling. These risk maps generated can assist managers in planning control measures for current infestations and assess the future risk of C. cinctus establishment in currently uninfested regions.

Colorado↗

A comprehensive assessment of membrane bioreactor contaminant removal efficacy through analytical chemistry, fish exposures, and microbiome characterization

Treated municipal wastewater effluent is an important pathway for Contaminants of Emerging Concern (CEC) to enter aquatic ecosystems. As the aging wastewater infrastructure in many industrialized countries requires upgrades or replacement, assessing new treatment technologies in the context of CEC effects may provide additional support for science-based resource management. Here, we used three lines of evidence, analytical chemistry, fish exposure experiments, and fish and water microbiome analysis, to assess the effectiveness of membrane bioreactor treatment (MBR) to replace traditional activated sludge treatment. To do this, we sampled a municipal wastewater treatment plant with a split wastewater stream, a portion of which was treated with an MBR and another via an oxidation ditch (OXI). The OXI and MBR treatments substantially reduced most measured CECs compared to the primary effluent (PRI). Only pesticides and some pharmaceuticals were recalcitrant to both secondary treatment methods. While ammonia toxicity of PRI prevented its inclusion in fish exposure experiments, exposure of fish with waters from the OXI or MBR treated wastewater produced only subtle biological differences with no adverse apical outcomes. These findings were consistent with low chemically derived exposure: activity ratios for OXI and MBR. Microbiome analysis of fish and wastewater highlighted the significant reduction of microbial abundance and diversity in the MBR treatment compared to all other treatments. The comparable removal efficacy of CECs in MBR makes it an attractive alternative to traditional OXI, especially when MBR may eliminate the need for tertiary treatment for wastewater disinfection.

Environmental Toxicology and Chemistry↗