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At least 109 records · Page 6Linked to original sources

The marine snail, Cerithidea californica, matures at smaller sizes where parasitism is high

I investigated life-history and parasitism in the salt marsh snail, Cerithidea californica. Latitude and growing conditions were important factors determining maturation size. After accounting for environmental variation, there was a negative association between the maturation size of snails and the prevalence of parasitic castration by larval trematodes. As predicted by life-history theory, this may represent an adaptation against parasitism that is similar to previous observations of life-history adaptations in species subject to predation or disturbance. However, it was unclear whether this adaptation was due to phenotypic plasticity or genetic differences among populations resulting from natural selection so I conducted a reciprocal transplant between sites with high and low prevalence and found source population differences in maturation size. It appears, therefore, that the life-history differences between these populations are at least partially genetic or may represent an adaptive developmental switch that was initiated prior to the transplant.

Oikos

Occurrence of volatile organic compounds in selected urban streams in the United States, 1995-2003

As part of the U.S. Geological Survey's (USGS) National Water-Quality Assessment (NAWQA) Program, urban indicator sites were monitored to (1) characterize the stream quality from drainage basins with predominantly residential and commercial land use, and (2) determine which selected natural and anthropogenic factors affect stream quality. A total of 869 water samples were collected from 37 urban streams during 1995-2003 and were analyzed for 87 volatile organic compounds (VOCs). The occurrence of VOCs in urban streams is described in this report for (1) all samples as a single dataset, (2) all samples grouped by streamflow pentiles, and (3) all samples grouped by warmer (April through September) and cooler (October through March) months by the detection frequency and (or) concentration of (a) any VOC, (b) VOC groups, and (c) individual compounds. An assessment level of 0.02 microgram per liter (ug/L) was used to compute the detection frequencies and concentrations of VOCs. Concentrations of VOCs were compared to (1) U.S. Environmental Protection Agency's (USEPA) drinking-water Maximum Contaminant Levels (MCLs) or Drinking Water Advisories, (2) Health-Based Screening Levels (HBSLs) developed by the USGS in collaboration with the USEPA and other agencies, and (3) USEPA and Canadian aquatic-life criteria. One or more VOCs were detected in 97.1 percent of 869 samples, and one or more VOCs were detected frequently (greater than 80 percent) at all sites. The median total VOC concentration for all samples was 0.57 ug/L, and total VOC concentrations in a single sample ranged from not detected to 698 ug/L. About 85 percent of the samples contained two or more VOCs, and about one-half contained five or more VOCs. The gasoline hydrocarbons were the most frequently occurring VOC group followed by solvents, trihalomethanes (THMs), gasoline oxygenates, organic synthesis compounds, fumigants, and refrigerants. Concentration ranges for most VOC groups were distributed over at least two orders of magnitude. Fifty-seven of the 87 VOCs analyzed were detected in at least one sample at an assessment level of 0.02 ug/L. More than one-half of the 30 VOCs not detected in samples were organic synthesis compounds. Fifteen compounds had detection frequencies greater than or equal to 10 percent. With the exception of toluene and chloroform, the median concentration of each VOC for all samples was less than the assessment level. Furthermore, the median concentrations of detections for the 15 most frequently occurring VOCs ranged from 0.03 to 3.9 ug/L, and typically were less than or equal to 0.10 ug/L. The 869 samples from the 37 sites were stratified into five streamflow pentiles (less than 20, 20-less than 40, 40-less than 60, 60-less than 80, and greater than or equal to 80 percent of estimated long-term streamflow statistics) for comparison of the occurrence of VOCs. The detection frequency of one or more VOCs by streamflow pentile varied only slightly from 96.7 to 97.7 percent. The median total VOC concentrations in samples for the five streamflow pentiles ranged from 0.39 to 1.0 ug/L. Two or more VOCs were present in more than 80 percent of samples in each of the five pentiles. The gasoline hydrocarbons, solvents, THMs, and gasoline oxygenates occurred frequently (greater than 30 percent) in all streamflow pentiles, in contrast to the organic synthesis compounds, fumigants, and refrigerants that occurred less frequently in urban streams under all streamflow conditions. The median total VOC concentrations for gasoline hydrocarbons, solvents, gasoline oxygenates, and organic synthesis compounds generally increased as streamflow increased. In contrast, the median total VOC concentrations for THMs and fumigants generally decreased as streamflow increased. The median total VOC concentrations for refrigerants showed no pattern as streamflow increased. Because differences between VOC occurrence and streamflow pentiles were small for most compariso

Scientific Investigations Report

Pesticides in Wyoming Groundwater, 2008-10

Groundwater samples were collected from 296 wells during 1995-2006 as part of a baseline study of pesticides in Wyoming groundwater. In 2009, a previous report summarized the results of the baseline sampling and the statistical evaluation of the occurrence of pesticides in relation to selected natural and anthropogenic (human-related) characteristics. During 2008-10, the U.S. Geological Survey, in cooperation with the Wyoming Department of Agriculture, resampled a subset (52) of the 296 wells sampled during 1995-2006 baseline study in order to compare detected compounds and respective concentrations between the two sampling periods and to evaluate the detections of new compounds. The 52 wells were distributed similarly to sites used in the 1995-2006 baseline study with respect to geographic area and land use within the geographic area of interest. Because of the use of different types of reporting levels and variability in reporting-level values during both the 1995-2006 baseline study and the 2008-10 resampling study, analytical results received from the laboratory were recensored. Two levels of recensoring were used to compare pesticides—a compound-specific assessment level (CSAL) that differed by compound and a common assessment level (CAL) of 0.07 microgram per liter. The recensoring techniques and values used for both studies, with the exception of the pesticide 2,4-D methyl ester, were the same. Twenty-eight different pesticides were detected in samples from the 52 wells during the 2008-10 resampling study. Pesticide concentrations were compared with several U.S. Environmental Protection Agency drinking-water standards or health advisories for finished (treated) water established under the Safe Drinking Water Act. All detected pesticides were measured at concentrations smaller than U.S. Environmental Protection Agency drinking-water standards or health advisories where applicable (many pesticides did not have standards or advisories). One or more pesticides were detected at concentrations greater than the CAL in water from 16 of 52 wells sampled (about 31 percent) during the resampling study. Detected pesticides were classified into one of six types: herbicides, herbicide degradates, insecticides, insecticide degradates, fungicides, or fungicide degradates. At least 95 percent of detected pesticides were classified as herbicides or herbicide degradates. The number of different pesticides detected in samples from the 52 wells was similar between the 1995-2006 baseline study (30 different pesticides) and 2008-2010 resampling study (28 different pesticides). Thirteen pesticides were detected during both studies. The change in the number of pesticides detected (without regard to which pesticide was detected) in groundwater samples from each of the 52 wells was evaluated and the number of pesticides detected in groundwater did not change for most of the wells (32). Of those that did have a difference between the two studies, 17 wells had more pesticide detections in groundwater during the 1995-2006 baseline study, whereas only 3 wells had more detections during the 2008-2010 resampling study. The difference in pesticide concentrations in groundwater samples from each of the 52 wells was determined. Few changes in concentration between the 1995-2006 baseline study and the 2008-2010 resampling study were seen for most detected pesticides. Seven pesticides had a greater concentration detected in the groundwater from the same well during the baseline sampling compared to the resampling study. Concentrations of prometon, which was detected in 17 wells, were greater in the baseline study sample compared to the resampling study sample from the same well 100 percent of the time. The change in the number of pesticides detected (without regard to which pesticide was detected) in groundwater samples from each of the 52 wells with respect to land use and geographic area was calculated. All wells with land use classified as agricultural had the same or a smaller number of pesticides detected in the resampling study compared to the baseline study. All wells in the Bighorn Basin geographic area also had the same or a smaller number of pesticides detected in the resampling study compared to the baseline study.

Wyoming

Statistical facilitation in environmental science: Integrating results from complementary statistical analyses can improve ecological interpretations

Professionals working in biological conservation seek to understand, manage, and restore populations of native organisms using many techniques. A common approach for this discipline is using long-term data collections to inform decision making. However, several quantitative issues complicate statistical analysis of monitoring datasets and can reduce the utility of results for conservation decision making. Integrating results from multiple analyses applied to the same dataset (i.e., approaching the same biological problem using different techniques) is one way to address concerns related to field data that violate statistical assumptions. This process allows data analysts, researchers, and managers to assemble insights based on the weight of evidence. Here we tested whether three different statistical techniques [(1) multiple logistic regression on original data, (2) multiple logistic regression on standardized data (i.e., mean of 0 and standard deviation of 1), and (3) random forest analysis] identified a similar hierarchy for selecting natural and anthropogenic habitat regressors. Our examination of how environmental variables affected Plains Minnow ( Hybognathus placitus ), a state-threatened fish, is relevant to other taxa and locations. We gained useful information from redundancies (i.e., agreements across analyses). New directions also emerged by addressing ambiguities (i.e., disagreements among results across analyses). When multiple analyses were integrated into one ecological story, a clearer interpretation emerged. Viewing different statistical tests as facilitators that provide mutual advantages can advance the understanding and application of statistical analyses applied to non-experimental field datasets.

Kansas

The role of genetics in chronic wasting disease of North American cervids

Chronic wasting disease (CWD) is a major concern for the management of North American cervid populations. This fatal prion disease has led to declines in populations which have high CWD prevalence and areas with both high and low infection rates have experienced economic losses in wildlife recreation and fears of potential spill-over into livestock or humans. Research from human and veterinary medicine has established that the prion protein gene ( Prnp ) encodes the protein responsible for transmissible spongiform encephalopathies (TSEs). Polymorphisms in the Prnp gene can lead to different prion forms that moderate individual susceptibility to and progression of TSE infection. Prnp genes have been sequenced in a number of cervid species including those currently infected by CWD (elk, mule deer, white-tailed deer, moose) and those for which susceptibility is not yet determined (caribou, fallow deer, sika deer). Over thousands of sequences examined, the Prnp gene is remarkably conserved within the family Cervidae; only 16 amino acid polymorphisms have been reported within the 256 amino acid open reading frame in the third exon of the Prnp gene. Some of these polymorphisms have been associated with lower rates of CWD infection and slower progression of clinical CWD. Here we review the body of research on Prnp genetics of North American cervids. Specifically, we focus on known polymorphisms in the Prnp gene, observed genotypic differences in CWD infection rates and clinical progression, mechanisms for genetic TSE resistance related to both the cervid host and the prion agent and potential for natural selection for CWD-resistance. We also identify gaps in our knowledge that require future research.

Prion

Heterozygosity and fitness: No strong association in Great Lakes populations of the zebra mussel, Dreissena Polymorpha (Pallas)

A number of studies have found positive associations between allozyme heterozygosity and fitness surrogates (e.g., body size and growth rate) for marine molluscs. We investigated whether similar relationships exist for freshwater populations of the zebra mussel, Dreissena polymorpha. Only one significant correlation between multi-locus heterozygosity and shell length was observed for a total of 22 D. polymorpha populations surveyed from midwestern U.S.A. lakes and streams, and the result was not significant on a table-wide basis. Meta-analysis revealed a significant common correlation coefficient (effect magnitude) between multi-locus heterozygosity and shell length across all 22 sites (rc = 0.052, P = 0.019, 1557 df). However, the variance in shell length explained by multi-locus heterozygosity was small (rc2 = 0.0027), implying a weak causal relationship if any. Also, we saw no relationship between heterozygosity and growth rate in a one-year field enclosure experiment. A significant heterozygosity-shell length correlation previously reported for a zebra mussel population at Put-in-Bay, Lake Erie, Ohio, may have been the product of unique population dynamics, rather than natural selection. Similar demographic considerations may contribute to inconsistencies in heterozygosity-fitness correlations seen for other molluscs.

Malacologia

Mangrove removal in the belize cays: effects on mangrove-associated fish assemblages in the intertidal and subtidal

We investigated the effects of mangrove cutting on fish assemblages in Twin Cays, Belize, in two habitat types. We conducted visual censuses at two sites in adjoining undisturbed/disturbed (30%–70% of shoreline fringe removed) sub-tidal fringing Rhizophora mangle Linnaeus, 1753. Observers recorded significantly more species and individuals in undisturbed sites, especially among smaller, schooling species (e.g., atherinids, clupeids), where densities were up to 200 times greater in undisturbed habitat. Multivariate analyses showed distinct species assemblages between habitats at both sites. In addition, extensive trapping with wire minnow traps within the intertidal zone in both undisturbed and disturbed fringing and transition (landward) mangrove forests was conducted. Catch rates were low: 638 individuals from 24 species over 563 trap-nights. Trap data, however, indicated that mangrove disturbance had minimal effect on species composition in either forest type (fringe/transition). Different results from the two methods (and habitat types) may be explained by two factors: (1) a larger and more detectable species pool in the subtidal habitat, with visual "access" to all species, and (2) the selective nature of trapping. Our data indicate that even partial clearing of shoreline and more landward mangroves can have a significant impact on local fish assemblages.

Twin Cays

Genetic differences between hatchery and wild steelhead for growth and survival in the hatchery and seaward migration after release (Study sites: Dworshak Hatchery and Clearwater Hatchery; Stocks: Dworshak hatchery and Selway River wild; Year classes: 1994 and 1995)

Various studies suggest that sea ranching of anadromous salmonids can result in domestication (increased fitness in the hatchery program) and a loss of fitness for natural production; however, the mechanism has not been characterized adequately. We artificially spawned hatchery and wild steelhead Oncorhynchus mykiss from the Clearwater River, Idaho, reared the resulting genetically marked (at the PEPA allozyme locus) progeny (HxH, HxW from hatchery females and wild males, and WxW) in hatcheries, and tested for differences in survival, growth, early maturation, downstream migration, and adult returns. Rearing treatments were mixed (crosses reared together) and separate (crosses reared separately from each other) at the hatchery of origin for the hatchery population where smolts are produced in one year, and at a nearby hatchery employing lower rations, lower winter temperatures, and two years of rearing to more closely mimic the natural life history (natural smolt age = 2-4 years). The hatchery population had been artificially propagated for six generations at the onset of our study. We found little or no difference in survival in the hatchery but substantially higher rates of growth and subsequent downstream migration for HxH than for WxW fish. Faster growth for HxH fish resulted in greater size at release which contributed to their higher migration rate, but other as yet uncharacterized traits also affected migration since the migration difference between crosses was apparent even within size classes. Growth of WxW fish was slower in the mixed than in the separate treatment indicating that WxW fish were competitively inferior to HxH fish in the hatchery environment. Incidence of precocious males was higher for WxW than for HxH fish in the separate but not in the mixed treatment. Incidence of HxH precocious males was similar between treatments. Apparently, the presence of HxH fish suppressed high incidence of early maturation by WxW males. A direct effect beyond the suppression of WxW growth by HxH fish was involved because the effect persisted within size categories. In-hatchery survival and growth of WxW relative to HxH fish may have been better with two-year rearing than in the standard one-year program (differences were consistent but small and non-significant); however, performance remained substantially worse for progeny of wild fish. Greater downstream migration for HxH than for WxW fish was primarily due to greater residualization for WxW than for HxH fish near the smolt release site rather than to immediate differential mortality. By August the residuals had lost condition compared to their condition in the hatchery the previous March, a month before release, and the residuals produced almost no smolts the following spring. Adult return rate was higher for HxH than for WxW fish for one year-class, consistent with the difference in downstream migration; only three adults (all WxW) returned from the other. Intermediate performance by HxW fish on growth, early maturation, downstream migration, and adult returns corroborated the genetic basis of the stock differences. Natural selection after release from the hatchery favored fish that performed well in the hatchery (grew fast, didn’t mature early, and excelled in other as yet uncharacterized traits) and genetically changed (domesticated) the wild population to resemble the hatchery population.

Idaho

Genetic and environmental influences on cold hardiness of native and introduced riparian trees

To explore latitudinal genetic variation in cold hardiness and leaf phenology, we planted a common garden of paired collections of native and introduced riparian trees sampled along a latitudinal gradient. The garden in Fort Collins, Colorado (latitude 40.6°N), included 681 native plains cottonwood (Populus deltoides subsp. monilifera) and introduced saltcedar (Tamarix ramosissima, T. chinensis, and hybrids) collected from 15 sites from 29.2 to 47.6°N in the central United States. In the common garden, both species showed latitudinal variation in fall, but not spring, leaf phenology. This suggests that latitudinal gradient field observations in fall phenology are a result, at least in part, of the inherited variation in the critical photoperiod. Conversely, the latitudinal gradient field observations in spring phenology are largely a plastic response to the temperature gradient. Populations from higher latitudes exhibited earlier bud set and leaf senescence. Cold hardiness varied latitudinally in both fall and spring for both species. Although cottonwood was hardier than saltcedar in midwinter, the reverse was true in late fall and early spring. The latitudinal variation in fall phenology and cold hardiness of saltcedar appears to have developed as a result of multiple introductions of genetically distinct populations, hybridization, and natural selection in the 150 years since introduction.

Conference Paper

Compilation of minimum and maximum isotope ratios of selected elements in naturally occurring terrestrial materials and reagents

Documented variations in the isotopic compositions of some chemical elements are responsible for expanded uncertainties in the standard atomic weights published by the Commission on Atomic Weights and Isotopic Abundances of the International Union of Pure and Applied Chemistry. This report summarizes reported variations in the isotopic compositions of 20 elements that are due to physical and chemical fractionation processes (not due to radioactive decay) and their effects on the standard atomic weight uncertainties. For 11 of those elements (hydrogen, lithium, boron, carbon, nitrogen, oxygen, silicon, sulfur, chlorine, copper, and selenium), standard atomic weight uncertainties have been assigned values that are substantially larger than analytical uncertainties because of common isotope abundance variations in materials of natural terrestrial origin. For 2 elements (chromium and thallium), recently reported isotope abundance variations potentially are large enough to result in future expansion of their atomic weight uncertainties. For 7 elements (magnesium, calcium, iron, zinc, molybdenum, palladium, and tellurium), documented isotope-abundance variations in materials of natural terrestrial origin are too small to have a significant effect on their standard atomic weight uncertainties. This compilation indicates the extent to which the atomic weight of an element in a given material may differ from the standard atomic weight of the element. For most elements given above, data are graphically illustrated by a diagram in which the materials are specified in the ordinate and the compositional ranges are plotted along the abscissa in scales of (1) atomic weight, (2) mole fraction of a selected isotope, and (3) delta value of a selected isotope ratio. There are no internationally distributed isotopic reference materials for the elements zinc, selenium, molybdenum, palladium, and tellurium. Preparation of such materials will help to make isotope ratio measurements among laboratories comparable. The minimum and maximum concentrations of a selected isotope in naturally occurring terrestrial materials for selected chemical elements reviewed in this report are given below: Isotope Minimum mole fraction Maximum mole fraction -------------------------------------------------------------------------------- 2H 0 .000 0255 0 .000 1838 7Li 0 .9227 0 .9278 11B 0 .7961 0 .8107 13C 0 .009 629 0 .011 466 15N 0 .003 462 0 .004 210 18O 0 .001 875 0 .002 218 26Mg 0 .1099 0 .1103 30Si 0 .030 816 0 .031 023 34S 0 .0398 0 .0473 37Cl 0 .240 77 0 .243 56 44Ca 0 .020 82 0 .020 92 53Cr 0 .095 01 0 .095 53 56Fe 0 .917 42 0 .917 60 65Cu 0 .3066 0 .3102 205Tl 0 .704 72 0 .705 06 The numerical values above have uncertainties that depend upon the uncertainties of the determinations of the absolute isotope-abundance variations of reference materials of the elements. Because reference materials used for absolute isotope-abundance measurements have not been included in relative isotope abundance investigations of zinc, selenium, molybdenum, palladium, and tellurium, ranges in isotopic composition are not listed for these elements, although such ranges may be measurable with state-of-the-art mass spectrometry. This report is available at the url: http://pubs.water.usgs.gov/wri014222.

Water-Resources Investigations Report

Synthesis of natural flows at selected sites in and near the Milk River basin, Montana, 1928-89

Natural monthly streamflows were synthesized for the years 1928-89 at 2 sites in the St. Mary River Basin and 11 sites in the Milk River Basin in north- central Montana. The sites are represented as nodes in a streamflow accounting model being developed by the Bureau of Reclamation for the Milk River Basin. Recorded flows at most sites have been affected by human activities, including reservoir storage and irrigation diversions. The flows at the model nodes were corrected for the effects of these activities to obtain synthesized flows. The synthesized flows at nodes with seasonal and short-term records were extended using a statistical technique. The methods of synthesis varied, depending on upstream activities and information available. Flows at sites in the St. Mary River Basin and at the Milk River at Eastern Crossing of International Boundary pre- viously had been synthesized. The flows at mainstem sites downstream from the Milk River at Eastern Crossing were synthesized by adding synthesized natural runoff from intervening drainage areas to natural flows for Milk River at Eastern Crossing. Natural runoff from intervening drainage areas was estimated by multiplying recorded flows at selected index gaging stations on tributary streams by the ratio of the intervening drainage area to the combined drainage area of the index stations. The recorded flows for Milk River at Western Crossing of International Boundary and for Peoples Creek near Dodson, Montana, were assumed to be natural flows. The synthesized annual flows at the mouth of the Milk River compared favorably with the recorded flows near the mouth when the effects of upstream irrigation were considered.

Water-Resources Investigations Report

Synthesis of monthly natural flows for selected sites in the Musselshell River basin, Montana, base period 1929-89

Synthesized monthly natural streamflows were required at 13 sites for use in a streamflow- accounting model to evaluate the effects of various water-allocation schemes on water availability in the Musselshell River Basin in central Montana. Records of monthly streamflow at 14 streamflow-gaging stations were used to synthesize monthly natural flows at tributaries and the 13 synthesis sites. A streamflow-record extension program was used to extend flow records at the 14 gaged sites to a common base period, 1929-89. To synthesize monthly natural flows at 10 sites on the Musselshell River mainstem, synthesized monthly natural flows at all signi- ficant tributary streams were required. Results from a previous study were used to synthesize tributary natural flows. Monthly natural flows at each mainstem site downstream from the first site were synthesized by successively adding monthly natural flows from intervening tributaries to the next upstream mainstem site. Special methods using extended-record flows from gaged tributaries were used to synthesize monthly natural flows at three tributary sites. Synthesized mean annual natural flows were found to be greater than mean annual extended-record flows at three selected comparison sites on the Musselshell River. The differences between mean natural and extended-record flows (depletions) at Harlowton and Musselshell were considered to be reasonable given the amount of irrigated acreage upstream from the two sites. The differences at Mosby, the site farthest downstream, was less than at Musselshell, the next upstream site, indicating that the methods of synthesis had error. The synthesis error generally was attributed to the larger natural variability of tributary flows in the lower portion of the Musselshell River Basin.

Water-Resources Investigations Report

Review of selected features of the natural system model, and suggestions for applications in south Florida

A study was conducted to review selected features of the Natural System Model, version 4.3. The Natural System Model is a regional-scale model that uses recent climatic data and estimates of historic vegetation and topography to simulate pre-canal-drainage hydrologic response in south Florida. Equations used to represent the hydrologic system and the numerical solution of these equations in the model were documented and reviewed. Convergence testing was performed using 1965 input data, and selected other aspects of the model were evaluated. Some conclusions from the evaluation of the Natural System Model include the following observations. Simulations were generally insensitive to the temporal resolution used in the model. However, reduction of the computational cell size from 2-mile by 2-mile to 2/3-mile by 2/3-mile resulted in a decrease in spatial mean ponding depths for October of 0.35 foot for a 3-hour time step. Review of the computer code indicated that there is no limit on the amount of water that can be transferred from the river system to the overland flow system, on the amount of seepage from the river to the ground-water system, on evaporation from the river system, or on evapotranspiration from the overland-flow system. Oscillations of 0.2 foot or less in simulated river stage were identified and attributed to a volume limiting function which is applied in solution of the overland-flow equations. The computation of the resistance coefficient is not consistent with the computation of overland-flow velocity. Ground-water boundary conditions do not always ensure a no-flow condition at the boundary. These inconsistencies had varying degrees of effects on model simulations, and it is likely that simulations longer than 1 year are needed to fully identify effects. However, inconsistencies in model formulations should not be ignored, even if the effects of such errors on model results appear to be small or have not been clearly defined. The Natural System Model can be a very useful tool for estimating pre-drainage hydrologic response in south Florida. The model includes all of the important physical processes needed to simulate a water balance. With a few exceptions, these hydrologic processes are represented in a reasonable manner using empirical, semiempirical, and mechanistic relations. The data sets that have been assembled to represent physical features, and hydrologic and meteorological conditions are quite extensive in their scope. Some suggestions for model application were made. Simulation results from the Natural System Model need to be interpreted on a regional basis, rather than cell by cell. The available evidence suggests that simulated water levels should be interpreted with about a plus or minus 1 foot uncertainty. It is probably not appropriate to use the Natural System Model to estimate pre-drainage discharges (as opposed to hydroperiods and water levels) at a particular location or across a set of adjacent computational cells. All simulated results for computational cells within about 10 miles of the model boundaries have a higher degree of uncertainty than results for the interior of the model domain. It is most appropriate to interpret the Natural System Model simulation results in connection with other available information. Stronger linkages between hydrologic inputs to the Everglades and the ecological response of the system would enhance restoration efforts.

Florida

Relation of desert pupfish abundance to selected environmental variables in natural and manmade habitats in the Salton Sea basin

We assessed the relation between abundance of desert pupfish, Cyprinodon macularius, and selected biological and physicochemical variables in natural and manmade habitats within the Salton Sea Basin. Field sampling in a natural tributary, Salt Creek, and three agricultural drains captured eight species including pupfish (1.1% of the total catch), the only native species encountered. According to Bray-Curtis resemblance functions, fish species assemblages differed mostly between Salt Creek and the drains (i.e., the three drains had relatively similar species assemblages). Pupfish numbers and environmental variables varied among sites and sample periods. Canonical correlation showed that pupfish abundance was positively correlated with abundance of western mosquitofish, Gambusia affinis, and negatively correlated with abundance of porthole livebearers, Poeciliopsis gracilis, tilapias (Sarotherodon mossambica and Tilapia zillii), longjaw mudsuckers, Gillichthys mirabilis, and mollies (Poecilia latipinnaandPoecilia mexicana). In addition, pupfish abundance was positively correlated with cover, pH, and salinity, and negatively correlated with sediment factor (a measure of sediment grain size) and dissolved oxygen. Pupfish abundance was generally highest in habitats where water quality extremes (especially high pH and salinity, and low dissolved oxygen) seemingly limited the occurrence of nonnative fishes. This study also documented evidence of predation by mudsuckers on pupfish. These findings support the contention of many resource managers that pupfish populations are adversely influenced by ecological interactions with nonnative fishes. ?? Springer 2005.

California

Historic and naturalized monthly streamflow for selected sites in the Red River of the North Basin in North Dakota, Minnesota, and South Dakota, 1931-2001

Historic monthly streamflow data were compiled and missing historic and naturalized monthly streamflow data were estimated to develop a database of updated streamflow data for January 1931 through December 2001 (the data-development period) for 35 sites in the Red River of the North Basin. Of the 35 sites, 4 had gaged historic monthly streamflow data for the entire data-development period, 10 had gaged historic monthly streamflow data for part of the data-development period, and 21 had no gaged historic monthly streamflow data. To develop the database, a modified drainage-area ratio method, a maintenance of variance extension type 1 method, and a water-balance method were used to estimate the missing historic monthly streamflow data. Naturalized streamflow for the 35 sites was estimated by eliminating the hydrologic effects of Orwell Dam, Reservation Dam, White Rock Dam, Baldhill Dam, surfacewater withdrawals, and return flows.

Scientific Investigations Report

Riverine turtles select habitats maintained by natural discharge regimes in an unimpounded large river

Turtle populations are imperiled worldwide, but limited ecological information from unaltered systems hampers science‐based management and conservation of some species, especially riverine turtles such as the spiny softshell ( Apalone spinifera ). We therefore investigated movements and spatial habitat selection of 54 A . spinifera in 633 river kilometres (rkm) of the least‐altered river in the conterminous United States—the Yellowstone River in Montana—from 2005 to 2009. Movement rates and home ranges were smaller than in fragmented, altered river systems because nesting and overwintering habitats were common and in close proximity. Habitat selection also differed. A . spinifera in the Yellowstone River overwintered in unaltered bluff pools and summered in complex reaches with side channels, islands, and diverse habitats. However, those in the highly altered Missouri River used deep alluvial pools for overwintering and flooded, inundated, or backwatered tributary mouths in spring and summer. Importantly, selected habitats in both rivers were functionally similar, including complex river reaches (with multiple channels, islands, and diverse habitats) and natural pool types. Unfortunately, these are the very habitats that are limited in rivers affected by dams, bank stabilization, and channelization. Therefore, preservation of natural and diverse riverine habitats—and the fluvial dynamics that maintain them—may enhance conservation of A . spinifera in large rivers.

Montana, North Dakota, South Dakota, Wyoming

Spatially explicit models of seasonal habitat for greater sage‐grouse at broad spatial scales: Informing areas for management in Nevada and northeastern California

Defining boundaries of species' habitat across broad spatial scales is often necessary for management decisions, and yet challenging for species that demonstrate differential variation in seasonal habitat use. Spatially explicit indices that incorporate temporal shifts in selection can help overcome such challenges, especially for species of high conservation concern. Greater sage‐grouse Centrocercus urophasianus (hereafter, sage‐grouse), a sagebrush obligate species inhabiting the American West, represents an important case study because sage‐grouse exhibit seasonal habitat patterns, populations are declining in most portions of their range and are central to contemporary national land use policies. Here, we modeled spatiotemporal selection patterns for telemetered sage‐grouse across multiple study sites (1,084 sage‐grouse; 30,690 locations) in the Great Basin. We developed broad‐scale spatially explicit habitat indices that elucidated space use patterns (spring, summer/fall, and winter) and accounted for regional climatic variation using previously published hydrographic boundaries. We then evaluated differences in selection/avoidance of each habitat characteristic between seasons and hydrographic regions. Most notably, sage‐grouse consistently selected areas dominated by sagebrush with few or no conifers but varied in type of sagebrush selected by season and region. Spatiotemporal variation was most apparent based on availability of water resources and herbaceous cover, where sage‐grouse strongly selected upland natural springs in xeric regions but selected larger wet meadows in mesic regions. Additionally, during the breeding period in spring, herbaceous cover was selected strongly in the mesic regions. Lastly, we expanded upon an existing joint–index framework by combining seasonal habitat indices with a probabilistic index of sage‐grouse abundance and space use to produce habitat maps useful for sage‐grouse management. These products can serve as conservation planning tools that help predict expected benefits of restoration activities, while highlighting areas most critical to sustaining sage‐grouse populations. Our joint–index framework can be applied to other species that exhibit seasonal shifts in habitat requirements to help better guide conservation actions.

California, Nevada