SEARCH · Geology Reports

Results for “Reproduction”

Search indexed USGS publications on groundwater, aquifers, geologic maps, mineral resources and earthquakes. Explore source records by subject and place.

SourceResearch connectionFrom yearThrough year

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 1,027 records · Page 57Linked to original sources

USGS2023

Why are larger fish farther upstream? Testing multiple hypotheses using Silver Chub in two Midwestern United States riverscapes

Objective Three competing hypotheses might explain the widely documented intrapopulation larger-fish-upstream phenomenon. The age-phased recruitment hypothesis posits that fish spawn downstream and move upstream as they age and grow, the static population with growth and mortality gradients hypothesis posits that fish spawn throughout a riverscape and growth is greater upstream while recruitment is greater downstream, and the colonization cycle hypothesis posits that fish spawn upstream, larvae drift downstream, and individuals move upstream as they age and grow. Methods We tested for the larger-fish-upstream pattern using populations of Silver Chub Macrhybopsis storeriana in the Arkansas and Ohio rivers, as well as investigated longitudinal variation in reproductive investment (Arkansas River), age structure for adult fish (Arkansas River), and number and occurrence of age-0 fish (Ohio River). Result The larger-fish-upstream pattern was temporally persistent in both riverscapes. In the Arkansas River, reproductive investment was greatest upstream, where initiation of spawning likely occurred based on gonadosomatic indices. Adult fish were most numerous in the Arkansas River 125–175 km upstream from Kaw Reservoir, with age-2 fish numbers peaking farther upstream compared with age-1 fish. In the Ohio River, age-0 fish counts increased downstream and were rare among the shortest river fragments (<100 km) between lock-and-dam structures. These findings are inconsistent with the age-phased recruitment hypothesis based on upstream spawning in the Arkansas River and inconsistent with the static population with growth and mortality gradients hypothesis based on virtual absence of age-2 fish downstream (Arkansas River) and age-0 fish upstream (Ohio River). The most likely explanation for longitudinal variation in Silver Chub size distribution is downstream drift of ichthyoplankton followed by net upstream movement (i.e., colonization cycle hypothesis), but formal assessments of movement and ova characteristics require more research. Conclusion Managing multidimensional riverscapes requires insight into the mechanisms that regulate upstream-to-downstream patterns in fish populations, and our work underscores a potential size-related benefit to maintaining broadscale longitudinal connectivity.

Arkansas River, Ohio River↗

USGS2012

Relationships between river discharge and abundance of age 0 redhorses ( Moxostoma spp.) in the Oconee River, Georgia, USA, with implications for robust redhorse

Robust redhorse ( Moxostoma robustum ) and notchlip redhorse ( M. collapsum ) are two species of redhorses that reside in the lower Oconee River, Georgia. Robust redhorse is listed as a state endangered species in Georgia and North Carolina, and attempts to investigate factors affecting its reproductive success have met with limited success. Therefore, catch of robust redhorse young were combined with catch of notchlip redhorse to increase sample size. These congeners with similar spawning repertoire were assumed to respond similarly to environmental conditions. River discharge during spawning and rearing seasons may affect abundance of both redhorses in the lower Oconee River. An information-theoretic approach was used to evaluate the relative support of models relating abundance of age 0 redhorses to monthly discharge statistics that represented magnitude, timing, duration, variability and frequency of river discharge events for April through June 1995–2006. The best-approximating model indicated a negative relationship between the abundance of redhorses and mean maximum river discharge and the number of high pulses during June as well as a positive relationship with intermediate duration of low flows during April–June. This model is 9.6 times more plausible than the next best-fitting model, which revealed a negative relationship between the abundance of redhorses and mean maximum river discharge during May and the number of high pulses during June as well as a positive relationship between abundance and intermediate duration of low flows during April–June. Management implications from the results indicate low-stable flows for at least a 2-week period during spawning and rearing may increase reproductive success of robust and notchlip redhorses.

River Research and Applications↗

USGS2014

Importance of reservoir tributaries to spawning of migratory fish in the upper Paraná River

Regulation of rivers by dams transforms previously lotic reaches above the dam into lentic ones and limits or prevents longitudinal connectivity, which impairs access to suitable habitats for the reproduction of many migratory fish species. Frequently, unregulated tributaries can provide important habitat heterogeneity to a regulated river and may mitigate the influence of impoundments on the mainstem river. We evaluated the importance of tributaries to spawning of migratory fish species over three spawning seasons, by comparing several abiotic conditions and larval fish distributions in four rivers that are tributaries to an impounded reach of the Upper Parana River, Brazil. Our study confirmed reproduction of at least 8 long-distance migrators, likely nine, out of a total of 19 occurring in the Upper Parana River. Total larval densities and percentage species composition differed among tributaries, but the differences were not consistent among spawning seasons and unexpectedly were not strongly related to annual differences in temperature and hydrology. We hypothesize that under present conditions, densities of larvae of migratory species may be better related to efficiency of fish passage facilities than to temperature and hydrology. Our study indicates that adult fish are finding suitable habitat for spawning in tributaries, fish eggs are developing into larvae, and larvae are finding suitable rearing space in lagoons adjacent to the tributaries. Our findings also suggest the need for establishment of protected areas in unregulated and lightly regulated tributaries to preserve essential spawning and nursery habitats.

River Research and Applications↗

USGS2006

Evaluation of energy expenditure in adult spring Chinook salmon migrating upstream in the Columbia River Basin: an assessment based on sequential proximate analysis

The upstream migration of adult anadromous salmonids in the Columbia River Basin (CRB) has been dramatically altered and fish may be experiencing energetically costly delays at dams. To explore this notion, we estimated the energetic costs of migration and reproduction of Yakima River-bound spring Chinook salmon Oncorhynchus tshawytscha using a sequential analysis of their proximate composition (i.e., percent water, fat, protein, and ash). Tissues (muscle, viscera, and gonad) were sampled from fish near the start of their migration (Bonneville Dam), at a mid point (Roza Dam, 510 km upstream from Bonneville Dam) and from fresh carcasses on the spawning grounds (about 100 km above Roza Dam). At Bonneville Dam, the energy reserves of these fish were remarkably high, primarily due to the high percentage of fat in the muscle (18-20%; energy content over 11 kJ g-1). The median travel time for fish from Bonneville to Roza Dam was 27 d and ranged from 18 to 42 d. Fish lost from 6 to 17% of their energy density in muscle, depending on travel time. On average, fish taking a relatively long time for migration between dams used from 5 to 8% more energy from the muscle than faster fish. From the time they passed Bonneville Dam to death, these fish, depending on gender, used 95-99% of their muscle and 73-86% of their viscera lipid stores. Also, both sexes used about 32% of their muscular and very little of their visceral protein stores. However, we were unable to relate energy use and reproductive success to migration history. Our results suggest a possible influence of the CRB hydroelectric system on adult salmonid energetics.

Oregon, Washginton↗

USGS2024

Assessing predictions from optimal egg theory for an ectotherm relative to habitat duration

Optimal egg size theory predicts females must balance investment per offspring to maximize fitness based on environmental quality. In wetlands, environmental quality can be duration of water and predator presence. Ectotherms using habitats that dry or contain predators are likely under selection to optimize offspring production. We measured reproductive output of wood frogs ( Rana sylvatica ) in 30 wetlands in Subarctic Canada, where rapid climate changes are accelerating wetland drying. We predicted wetlands with short hydroperiods would have larger ova, smaller clutch sizes, and larger ovum-to-clutch-sizes than wetlands with long hydroperiods or with fish predators. We found partial support for predictions with larger ova in habitats with short hydroperiods and no fish but no evidence of larger clutch sizes in wetlands with fish. Our study implicates changes to wetland hydroperiod as a source of plasticity affecting one aspect of reproductive effort (ovum size) in an ectotherm but not another (clutch size).

Manitoba↗

USGS2021

Field methods for translocating female greater sage-grouse (Centrocercus urophasianus) with their broods

Greater sage-grouse ( Centrocercus urophasianus ) have experienced considerable range contraction and reduced abundance in response to habitat loss and degradation. Translocation is a conservation action that is often used to reintroduce extirpated populations or augment existing small populations. Translocations have had limited success in restoring viable populations of sage-grouse; a lack of success is attributed to long-distance post-release movements away from release sites, reduced survival, and lack of reproductive success of translocated individuals. Translocating female sage-grouse with their chicks (brood translocation) is a technique aimed at promoting breeding area fidelity and reproduction and may be beneficial to population restoration efforts. Furthermore, the ability to capture, relocate, and release individuals while minimizing translocation-induced loss increases the overall probability of restoration success. Accordingly, we developed a protocol to translocate female sage-grouse and their broods simultaneously, using a delayed-release system that included a custom release box and acclimation pen. We tested our protocol across 2 separate restoration projects in North Dakota and California during 2017–2019 with a total of 38 translocated females and 196 chicks. We successfully released 174/196 chicks (88.8%) from 32/38 (84.2%) broods. Our protocol builds on existing translocation methods used to translocate sage-grouse and will likely prove to be a critical technique in restoring sage-grouse populations.

California, North Dakota, Wyoming↗

USGS2017

Assessment of a strain 19 brucellosis vaccination program in elk

Zoonotic diseases in wildlife present substantial challenges and risks to host populations, susceptible domestic livestock populations, and affected stakeholders. Brucellosis, a disease caused by the bacterium Brucella abortus , is endemic among elk ( Cervus canadensis ) attending winter feedgrounds and adjacent areas of western Wyoming, USA. To minimize transmission of brucellosis from elk to elk and elk to livestock, managers initiated a B. abortus strain 19 ballistic vaccination program in 1985. We used brucellosis prevalence (1971–2015) and reproductive outcome (2006–2015) data collected from female elk attending feedgrounds to assess efficacy of the strain 19 program while controlling for potentially confounding factors such as site and age. From our generalized linear models, we found that seroprevalence of brucellosis was 1) not lower following inception of vaccination; 2) not inversely associated with proportion of juveniles vaccinated over time; 3) not inversely associated with additional yearlings and adults vaccinated over time; and 4) associated more with feeding end-date than proportion of juveniles vaccinated. Using vaginal implant transmitters in adult females that were seropositive for brucellosis, we found little effect of vaccination coverage at reducing reproductive failures (i.e., abortion or stillbirth). Because we found limited support for efficacy of the strain 19 program, we support research to develop an oral vaccine and suggest that continuing other spatio-temporal management actions will be most effective to minimize transmission of brucellosis and reduce dependency of elk on supplemental winter feeding.

Idaho, Wyoming↗

USGS1999

Forced copulation results in few extrapair fertilizations in Ross's and lesser snow geese

Extrapair paternity varies from 0 to over 70% of young among various populations of birds. Comparative studies have suggested that this variation is related to nesting density, breeding synchrony and the proportion of extrapair copulations. We used minisatellite DNA fingerprinting to examine levels of extrapair paternity in Ross's geese, Chen rossi, and lesser snow geese, C. caerulescens c. (hereafter snow geese) nesting in the largest known goose colony in the world. These geese have one of the highest known percentages of extrapair copulation (46-56% of all attempted copulations), and all of these appeared to be forced. Among all successful copulations, 33 and 38% were extrapair in Ross's and snow geese, respectively. Despite the high percentage of extrapair copulations, extrapair paternity was low in both Ross's and snow geese (2-5% of young). Extrapair paternity was not related to nest density in either species. However, in snow geese, extrapair paternity was more likely to occur in nests of females that nested asynchronously, either early or late in the season. This is one of a few reported examples of a negative relationship between extrapair paternity and breeding synchrony. Extrapair young also tended to come from eggs laid later in the clutch. Although forced extrapair copulations appear to be a relatively inefficient reproductive tactic for males, they may provide a reproductive advantage for some males.

Animal Behaviour↗

USGS2000

Mate fidelity and breeding site tenacity in a monogamous sandpiper, the black turnstone

We examined the relationship between mate fidelity and breeding site tenacity during a 5-year study of the black turnstone, Arenaria melanocephala, a socially monogamous sandpiper breeding in subarctic Alaska. We tested the predictions of several hypotheses regarding the incidence of divorce and the benefits of fidelity to mate and breeding site. Interannual return rates to the breeding grounds (88% for males, 79% for females) were among the highest yet recorded for any scolopacid sandpiper, and 88% of returning birds nested on their previous year's territory. The annual divorce rate was only 11%, and mate fidelity was significantly linked to fidelity to territory but independent of sex and year. Males arrived in spring significantly earlier than their mates and interannual fidelity was influenced by the relative timing of arrival of pair members. Reunited pairs had significantly higher fledging success than new pairs formed after death or divorce. The incidence of divorce was unrelated to reproductive success the previous year, although birds nested significantly further away after failure than after a successful nesting attempt. Sightings of marked individuals suggested that members of pairs do not winter together, and breeding site tenacity provides a mechanism through which pair members can reunite. We reject the 'incompatibility' hypothesis for divorce in turnstones, and our data contradict predictions of the 'better option' hypothesis. Alternatively, we propose the 'bet-hedging' hypothesis to explain the occurrence of divorce, which transpires when an individual pairs with a new mate to avoid the cost of waiting for a previous mate to return. Such costs can include remaining unmated, if the former mate has died, or experiencing lower reproductive success because of delayed breeding.

Animal Behaviour↗

USGS1997

Succession of desert plants on debris flow terraces, Grand Canyon, Arizona, U.S.A.

Vegetation sampling on 11 debris flow terraces in Grand Canyon National Park, Arizona, U.S.A., showed that plant assemblages changed as age of surface increased. The terraces ranged in age from about 5 to about 3100 years. There were distinct differences among sites in the life history characteristics of the dominant plants. Young terraces (5-55 years) were dominated by short-lived plants that had high reproductive potential. Older surfaces were dominated by species with longer life-spans and lower reproductive potential. Density and cover of long-lived species increased with age of surface; for short-lived plants, density was inversely related to surface age. Species composition was also correlated with site age; however, location, exposure, and other factors ensured that no two debris flows supported identical mixtures of species. Succession on recent Grand Canyon debris flows is driven in part by life-history strategies, particularly life-span and seed dispersal traits, and also by climatic factors, especially those that control germination and establishment of the long-lived dominants.

Journal of Arid Environments↗

USGS2000

Stress resistance strategy in an arid land shrub: interactions between developmental instability and fractal dimention

This paper investigates allocation of energy to mechanisms that generate and preserve architectural forms (i.e. developmental stability, complexity of branching patterns) and productivity (growth and reproduction) in response to environmental disturbances (i.e. grazing and resource availability). The statistical error in translational symmetry was used to detect random intra-individual variability during development. This can be thought of as a measure of developmental instability caused by stress. Additionally, we use changes in fractal complexity and shoot distribution of branch structures as an alternate indicator of stress. These methods were applied to Anthyllis cytisoides L., a semi-arid environment shrub, to ascertain the effect of grazing and slope exposure on developmental traits in a 2×2 factorial design. The results show that A. cytisoides maintains developmental stability at the expense of productivity. Anthyllis cytisoides was developmentally more stable when grazed and when on south-facing, as opposed to north-facing slopes. On the contrary, shoot length, leaf area, fractal dimension and reproductive-to-vegetative allocation ratio were larger in north- than in south-facing slopes. As a consequence, under extreme xeric conditions, shrub mortality increased in north-facing slopes, especially when not grazed. The removal of transpiring area and the reduction of plant competition favoured developmental stability and survival in grazed plants. Differences between grazed and ungrazed plants were most evident in more mesic (north-facing) areas.

Journal of Fish Diseases↗

USGS2004

Mercury hazards from gold mining to humans, plants, and animals

Mercury contamination of the environment from historical and ongoing mining practices that rely on mercury amalgamation for gold extraction is widespread. Contamination was particularly severe in the immediate vicinity of gold extraction and refining operations; however, mercury, especially in the form of water-soluble methylmercury, may be transported to pristine areas by rainwater, water currents, deforestation, volatilization, and other vectors. Examples of gold mining-associated mercury pollution are shown for Canada, the United States, Africa, China, the Philippines, Siberia, and South America. In parts of Brazil, for example, mercury concentrations in all abiotic materials, plants, and animals--including endangered species of mammals and reptiles, collected near ongoing mercury-amalgamation gold mining sites were far in excess of allowable mercury levels promulgated by regulatory agencies for the protection of human health and natural resources. Although health authorities in Brazil are unable to detect conclusive evidence of human mercury intoxication, the potential exists in the absence of mitigation for epidemic mercury poisoning of the mining population and environs. In the United States, environmental mercury contamination is mostly from historical gold mining practices, and portions of Nevada remain sufficiently mercury-contaminated to pose a hazard to reproduction of carnivorous fishes and fish-eating birds. Concentrations of total mercury lethal to sensitive representative natural resources range from 0.1 to 2.0 microg/L of medium for aquatic organisms; from 2,200 to 31,000 microg/kg BW (acute oral) and from 4,000 to 40,000 microg/kg (dietary) for birds; and from 100 to 500 microg/kg BW (daily dose) and from 1,000 to 5,000 microg/kg diet for mammals. Significant adverse sublethal effects were observed among selected aquatic species at water concentrations of 0.03-0.1 microg Hg/L. For some birds, adverse effects, mainly on reproduction, have been associated with total mercury concentrations (microg/kg FW) of 5,000 in feathers, 900 in eggs, and 50-100 in diet, and with daily intakes of 640 microg/kg BW. Sensitive nonhuman mammals showed significant adverse effects of mercury when daily intakes were 250 microg/kg BW, when dietary levels were 1,100 microg/kg, or when tissue concentrations exceeded 1,100 microg/kg. Proposed mercury criteria for protection of aquatic life range from 0.012 microg/L for freshwater life to 0.025 microg/L for marine life; for birds, less than 100 microg/kg diet FW; and for small mammals, less than 1,100 microg/kg FW diet. All these proposed criteria provide, at best, minimal protection.

Archives of Environmental Contamination and Toxico↗

USGS1991

H4IIE rat hepatoma cell bioassay-derived 2,3,7,8-tetrachlorodibenzo-p-dioxin equivalents in colonial fish-eating waterbird eggs from the Great Lakes

Fish-eating waterbirds from the Great Lakes of North America have shown symptoms of poisoning similar to those observed in laboratory exposures of various avian species to planar halogenated hydrocarbons (PHHs). PHHs, include among others, polychlorinated biphenyls (PCBs), polychlorinated dibenzo- p -dioxins (PCDDs), and polychlorinated dibenzofurans (PCDFs) and have been implicated in some of the reproductive problems of Great Lakes waterbirds. The objectives of this study were to assess the overall potencies of PCB-containing extracts from colonial water-bird eggs taken from the Great Lakes and to compare the potencies with the location and spatial distribution of the colonies. The potencies of the extracts were assessed by their ability to induce cytochrome P450IA1-associated ethoxyresorufin O-deethylase (EROD) activity in H4IIE rat hepatoma cells as compared to the standard, 2,3,7,8-tetrachlorodibenzo- p -dioxin (TCDD). The H4IIE bioassay-derived TCDD-equivalents (TCCD-EQs) in the waterbird eggs concur with residue analyses and biological data from other studies. The greatest concentrations of TCDD-EQs were found in waterbird eggs from historically polluted, industrialized or urbanized areas in which the reproductive impairment of colonial waterbirds was most severe. However, significant concentrations of TCDD-EQs were detected at all sites tested; with a range of 49 to 415 pg TCDD-EQ/g egg, uncorrected for extraction efficiencies. The H4IIE bioassay proved to be a useful biomonitoring tool to assess the overall potency of complex PHH mixtures in environmental samples.

Beaver Island Group, Green Bay, Lake Erie, Lake Hu↗

USGS1982

Effects of chronic exposure to simazine on the cladoceran, Daphnia pulex

The effects of chronic exposure to simazine, an s-triazine herbicide, on the cladoceran Daphnia pulex were evaluated under laboratory conditions. The daphnids were exposed to simazine concentrations corresponding to previously calculated 48-hr LC5 and LC20 values and the effects on survival, growth, reproduction, and behavior were monitored daily for 26 days. Survival of D. pulex in 20 mg/L simazine averaged 9.6±1.7 days. Most mortality in 4 mg/L occurred between days 21 and 25, and 65% of the daphnids were dead by day 25. Simazine interfered with molting, and growth was reduced in both concentrations tested. Reproductive maturation was delayed by about one molt cycle (3 days at 20°C) in D. pulex exposed to 4 mg/L simazine and was prevented in daphnids exposed to 20 mg/L. Numbers of live young and broods produced by daphnids in 4 mg/L were significantly lower than those produced by controls.

Archives of Environmental Contamination and Toxico↗

USGS1983

Breeding success of barn owls (Tyto alba) fed low levels of DDE and dieldrin

The relative importance of two organochlorine pesticides in the recent reproductive failure of raptors was investigated. Captive barn owls were fed 3.0 ppm DDE and 0.5 ppm dieldrin; doses were given separately and in combination for two years. Breeding success was followed from the laying of eggs through natural incubation and rearing of young. DDE was associated with significant eggshell thinning, egg breakage, embryo mortality, and reduced production per pair. Dieldrin alone was associated with slight but significant eggshell thinning, but not with reduction of breeding success. Ecological implications of the results are discussed; it is suggested that DDE had a much more severe effect on reproduction in wild raptors than dieldrin, which contributed to their decline primarily through adult mortality.

Archives of Environmental Contamination and Toxico↗

USGS1989

Contaminants in foods of aquatic birds at Kesterson Reservoir, California, 1985

Plants, aquatic insects, and mosquitofish ( Gambusia affinis ) were collected from Kesterson Reservoir, Merced County, California, and a nearby reference site (Volta Wildlife Area) to compare concentrations of three contaminants found in 1985 with those reported in 1983 and 1984. Mean selenium concentrations in food-chain organisms from sites at Kesterson in 1985 ranged from 26.0 μg/g (dry wt) in water boatman (Corixidae) to 119 μg/g in mosquitofish. All mean selenium concentrations at Kesterson were significantly higher than those from Volta and were sufficient to have caused the impaired avian reproduction observed at Kesterson. Boron concentrations were also significantly higher at Kesterson, and, at one pond, the mean concentration in widgeongrass ( Ruppia mari-tima ) (1,630 μg/g) was high enough to impair avian reproduction. There were no differences in arsenic concentrations between locations, and concentrations in all food-chain organisms (<1.9 μg/g) were lower than those reported to cause adverse effects in wildlife. Within-location differences were observed for all three contaminants at Kesterson and for selenium at Volta, but there was no consistent pattern to these differences. Between-year comparisons showed that selenium concentrations in mosquitofish generally decreased at Kesterson, but remained about the same at Volta over the 3 years. Selenium concentrations in insects from 1985 were lower at Kesterson than 1983, but were similar to 1984. Concentrations in plants were generally higher in 1983 and lower in 1984 compared with 1985. Boron concentrations in plants were generally higher in 1985, but in mosquitofish and insects, boron concentrations remained about the same all 3 years. Most arsenic concentrations did not change significantly between years.

California↗

USGS1974

Effects of DDT on bobwhite quail adrenal gland

A wide range of responses to sublethal levels of DDT exist, many of which are species specific and vary within each species depending upon age, sex, and physiological state. Sublethal levels of DDT do cause an increase in the adrenal cortical tissue of bobwhite quail, which may cause increased secretion of corticosteroids, and in turn affect reproduction. A delicate homeostatic balance exists within the avian endocrine system which may be disturbed by feeding sublethal levels of chlorinated hydrocarbon pesticides. This adverse effect on the endocrine system may cause subtle reproductive failures which go unnoticed until the population is greatly reduced.

Bulletin of Environmental Contamination and Toxico↗

USGS2003

Predicting mercury in mallard ducklings from mercury in chorioallantoic membranes

Methylmercury has been suspected as a cause of impaired reproduction in wild birds, but the confounding effects of other environmental stressors has made it difficult to determine how much mercury in the eggs of these wild species is harmful. Even when a sample egg can be collected from the nest of a wild bird and the mercury concentration in that egg compared to the laboratory-derived thresholds for reproductive impairment, additional information on the mercury levels in other eggs from that nest would be helpful in determining whether harmful levels of mercury were present in the clutch. The measurement of mercury levels in chorioallantoic membranes offers a possible way to estimate how much mercury was in a chick that hatched from an egg, and also in the whole fresh egg itself. While an embryo is developing, wastes are collected in a sac called the chorioallantoic membranes, which often remain inside the eggshell and can be collected for contaminant analysis. We fed methylmercury to captive mallards to generate a broad range of mercury levels in eggs, allowed the eggs to hatch normally, and then compared mercury concentrations in the hatchling versus the chorioallantoic membranes left behind in the eggshell. When the data from eggs laid by mercury- treated females were expressed as common logarithms, a linear equation was created by which the concentration of mercury in a duckling could be predicted from the concentration of mercury in the chorioallantoic membranes from the same egg. Therefore, if it were not possible to collect a sample egg from a clutch of wild bird eggs, the collection of the chorioallantoic membranes could be substituted, and the mercury predicted to be in the chick or whole egg could be compared to the thresholds of mercury that have been shown to cause harm in controlled feeding studies with pheasants, chickens, and mallards.

Bulletin of Environmental Contamination and Toxico↗