Assessing predictions from optimal egg theory for an ectotherm relative to habitat duration

Abstract

Optimal egg size theory predicts females must balance investment per offspring to maximize fitness based on environmental quality. In wetlands, environmental quality can be duration of water and predator presence. Ectotherms using habitats that dry or contain predators are likely under selection to optimize offspring production. We measured reproductive output of wood frogs ( Rana sylvatica ) in 30 wetlands in Subarctic Canada, where rapid climate changes are accelerating wetland drying. We predicted wetlands with short hydroperiods would have larger ova, smaller clutch sizes, and larger ovum-to-clutch-sizes than wetlands with long hydroperiods or with fish predators. We found partial support for predictions with larger ova in habitats with short hydroperiods and no fish but no evidence of larger clutch sizes in wetlands with fish. Our study implicates changes to wetland hydroperiod as a source of plasticity affecting one aspect of reproductive effort (ovum size) in an ectotherm but not another (clutch size).