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180 records · Page 4Linked to original sources
USGS2025

Emerging investigator series: Post-wildfire sediment geochemical characterization reveals manganese reactivity and a potential link to water quality impairment in the Gallinas Creek watershed, New Mexico

Water quality post-wildfire is often impaired by increased turbidity and elevated concentrations of elements such as manganese (Mn) and iron (Fe). Precipitation events exacerbate these issues, due in part to increased erosion and transport of sediment from hillslopes to surface water. Both Mn and Fe are major redox-active elements in sediments that drive a variety of biogeochemical cycles, precipitate adsorptive phases, and can themselves be drinking water contaminants. By investigating Mn and Fe sediment geochemistry in post-wildfire sediment deposits, related water quality hazards can be assessed. To establish and strengthen this connection, we analyzed the geochemistry of sediment deposits and surface water in the Gallinas Creek watershed, New Mexico over 1.5 years post-wildfire. Analyses included particle size analysis, water extractions, sequential extractions and aqua regia extractions to determine metal partitioning in sediment deposits. Data demonstrate Mn concentrations were distributed across labile and reactive fractions, such as the exchangeable and oxyhydroxide fractions, while Fe concentrations were mainly associated with the residual fraction. Manganese concentrations in aqua regia extractions and several fractions of sequential extractions were also strongly and significantly correlated with fine-grained sediment while the same pools of Fe concentrations were not. Dissolved Mn concentrations in surface water were elevated (>50 μg L −1 ) multiple times over the 1.5 years post-wildfire, highlighting a relationship between sediment geochemistry and water quality. This work shows Mn in sediments mobilized post-wildfire has an influence on water quality and highlights how further investigation into Mn sediment redox processes and mineralogy post-wildfire can inform risk assessments and resource management.

New Mexico↗
USGS2026

Baseflow and snowmelt sustained streamflow in the Upper Colorado River Basin, 1986-2020

The Upper Colorado River Basin (UCRB) faces substantial water availability limitations. Although most streamflow originates as snowmelt, the partitioning of snowmelt between surface runoff and groundwater recharge and subsequent groundwater discharge to streams is highly uncertain. On average, over half of the streamflow in the UCRB is estimated to originate from groundwater discharge to streams, highlighting the importance of baseflow in sustaining surface water. However, the historical patterns of baseflow and streamflow, along with their variability over space and time and their specific sources, remain unknown at the basin scale. This study addresses those gaps by characterizing the sources and transport pathways of both baseflow and streamflow in the UCRB at a seasonal timestep from 1986 to 2020, including the lagged delivery of subsurface water to streams beyond the current season, using coupled models of baseflow and streamflow. Between 1986 and 2020, on average 63% of UCRB streamflow originated from baseflow. About half of this baseflow took longer than one season to reach streams, and outside the snowmelt season, baseflow was the dominant source of streamflow. Snowmelt was a key source of both baseflow and streamflow. Current season snowmelt contributed 33% of streamflow via runoff, and 22% of the 29% of streamflow that originated as current season baseflow via subsurface flow to streams. Over the study period, baseflow index (BFI) declined in headwaters and increased at mid-elevations. Springtime increases in BFI demonstrate the increasingly important role baseflow plays in water supply. Identifying the sources, locations, and timing of water that contributed to the UCRB outlet can inform management of water resources in the basin.

Arizona, Colorado, New Mexico, Utah, Wyoming↗
USGS2025

Wet meadow regeneration through restoration of biophysical feedbacks

Wet meadows are globally significant ecosystems that provide critical hydrological, ecological, and biogeochemical functions, yet their extent has declined dramatically due to land use changes and hydrologic alteration. These sedge-dominated wetlands exist at the drier end of the wetland gradient, maintained by shallow groundwater and periodic inundation. This paper is a global synthesis of the ecological, geomorphic, and hydrological dynamics of wet meadows, with an emphasis on alluvial systems, to inform effective restoration strategies. We compare wet meadows to other wetlands, classify them into palustrine, lacustrine, and alluvial types, then focus on alluvial wet meadows and discuss how their formation and persistence depend on ground and surface water interactions, sediment deposition and flow obstructions, all mediated by biological processes. In particular, we highlight the role of hydric graminoids in resisting erosion and maintaining soil cohesion, how beaver promote meadow persistence, and the significance of wet meadows as carbon sinks. We also present stratigraphic evidence demonstrating that incision, often triggered by anthropogenic activity or changing climate, is the primary mechanism of alluvial wet meadow degradation, resulting in water table decline and shifts in vegetation composition. Restoration requires reversing these incisional processes through techniques that elevate water tables, disperse flow and retain sediment—methods traditionally associated with either soil conservation or stream restoration. These include nature-based solutions that create obstructions such as beaver dams and their analogues, rock and wood-based obstructions and incision trench or gully filling and grading. Given their multifunctional value—including but not limited to flood attenuation, biodiversity support, and carbon sequestration—wet meadows warrant a focused restoration framework. This review advocates for a valley-floor scale restoration paradigm that integrates hydrological reconnection, sediment retention, and biological reinforcement to ensure long-term resilience of these systems in the face of changing climate and land use pressures.

Frontiers in Environmental Science↗
USGS2026

Keeping pace: Migrating sea lampreys adjust swim speed in response to shifting water velocity to maintain a constant ground speed

Upstream migrating fish must balance energetic costs with environmental and ecological constraints. Theoretical models predict that fish can conserve energy by adjusting swim speed in response to changing water velocity, but detailed field tests of these predictions remain rare. We used fine-scale acoustic telemetry and hydrodynamic modeling to track adult sea lamprey (Petromyzon marinus) migrating through a river and evaluated how movement speeds varied with environmental conditions and individual traits. Swim speed increased with faster water velocities, while ground speed remained constant, supporting the hypothesis that lampreys regulate swim effort to maintain a fixed migration pace. The average ground speed aligned with a predicted optimum of ∼1 body length per second. Both swim and ground speeds declined slightly in deeper water, suggesting a trade-off between movement efficiency and predator avoidance. These findings provide field-based support for a theoretical model of energy optimization and underscore how migratory fish adaptively adjust behavior to local hydrodynamic conditions. Our results highlight the value of using high-resolution tracking and environmental modeling to reveal behavioral tactics that underlie efficient migration strategies.

Michigan↗
USGS2026

Restoring dryland water cycles for precipitation feedback and climate stability; a review

Drylands across the globe are experiencing intensifying water scarcity, land degradation, and hydroclimatic extremes. This review integrates evidence from multidecadal field studies, hydrologic monitoring, geomorphic and ecological assessments, remote sensing, and land–atmosphere science to evaluate how restoration influences key components of the terrestrial water cycle. Low-tech natural infrastructure in dryland streams (NIDS)—including check dams, leaky weirs, one-rock dams, and gabions—has emerged as a promising but under-synthesized nature-based solution for restoring hydrologic function in these environments. We describe the mechanisms through which these interventions modify runoff detention, infiltration, sediment and alluvial storage, shallow-groundwater recharge, vegetation recovery, and surface-energy partitioning, and we summarize outcomes across diverse dryland settings. Findings consistently show increased water residence time, enhanced soil-moisture storage, expanded riparian vegetation, extended flow duration, and shifts toward greater latent-heat flux—producing localized cooling and strengthened ecohydrological feedbacks. Building on these localized effects, we articulate a hypothesis that links the spatial extent of restoration, the density of NIDS per unit drainage area, and the magnitude of the latent-to-sensible-heat contrast generated by wetter post-rainfall conditions. Specifically, we hypothesize that when NIDS are implemented at densities permitted by topography and across areas large enough to maintain elevated soil moisture after storm events, the resulting increases in latent heat flux, surface cooling, and boundary-layer moistening may enhance moisture convergence and boundary-layer development, potentially increasing the likelihood or stability of convective precipitation, analogous to how reductions in these processes have contributed to regional drought intensification. These land–atmosphere feedbacks remain untested at scale but represent an important research Frontier. By integrating hydrologic, geomorphic, ecological, and atmospheric perspectives, this review provides a comprehensive framework for considering how low-tech, landscape-scale interventions can strengthen watershed resilience and contribute to climate-relevant nature-based solutions.

Arizona↗
USGS2025

Tapwater exposures, residential risk, and mitigation in a PFAS-impacted-groundwater community

Tapwater (TW) safety and sustainability are priorities in the United States. Per/polyfluoroalkyl substance(s) (PFAS) contamination is a growing public-health concern due to prolific use, widespread TW exposures, and mounting human-health concerns. Historically-rural, actively-urbanizing communities that rely on surficial-aquifer private wells incur elevated risks of unrecognized TW exposures, including PFAS, due to limited private-well monitoring and contaminant-source proliferation in urbanizing landscapes. Here, a broad-analytical-scope TW-assessment was conducted in a hydrologically-vulnerable, Mississippi River alluvial-island community, where PFAS contamination of the shallow-alluvial drinking-water aquifer has been documented, but more comprehensive contaminant characterization to inform decision-making is currently lacking. In 2021, we analyzed 510 organics, 34 inorganics, and 3 microbial groups in 11 residential and community locations to assess (1) TW risks beyond recognized PFAS issues, (2) day-to-day and year-to-year risk variability, and (3) suitability of the underlying sandstone aquifer as an alternative source to mitigate TW-PFAS exposures. Seventy-six organics and 25 inorganics were detected. Potential human-health risks of detected TW exposures were explored based on cumulative benchmark-based toxicity quotients ( ∑ TQ ). Elevated risks ( ∑ TQ ≥ 1) from organic and inorganic contaminants were observed in all alluvial-aquifer-sourced synoptic samples but not in sandstone-aquifer-sourced samples. Repeated sampling at 3 sites over 52–55 h indicated limited variability in risk over the short-term. Comparable PFAS-specific ∑ TQ for spatial-synoptic, short-term (3 days) temporal, and long-term (3 years quarterly) temporal samples indicated that synoptic results provided useful insight into the risks of TW-PFAS exposures at French Island over the long-term. No PFAS detections in sandstone-aquifer-sourced samples over a 3 year period indicated no PFAS-associated risk and supported the sandstone aquifer as an alternative drinking-water source to mitigate community TW-PFAS exposures. This study illustrated the importance of expanded contaminant monitoring of private-well TW, beyond known concerns (in this case, PFAS), to reduce the risks of a range of unrecognized contaminant exposures.

Wisconsin↗
USGS2025

U.S. Geological Survey global seabed mineral resources

The U.S. Geological Survey (USGS) provides science and data on seabed mineral resources and ecosystems, as well as on the potential hazards associated with extraction. The Nation relies on minerals for infrastructure, technology, manufacturing, and energy production. Critical minerals are essential to the economic and national security of the United States and have a supply chain vulnerable to disruption. For decades, USGS scientific innovation has contributed to the delineation of seabed mineral resources, the mechanisms of seabed mineral formation, and the environmental impacts of resource extraction. Since 1962, the USGS has also led scientific inquiries into the potential for deep sea mining. By providing impartial science on seabed minerals and their environmental setting in the deep oceans, the USGS enables decision-makers to evaluate the best practices for mineral resource development.

Fact Sheet↗
USGS2026

Utilizing hydrophones to detect streambed mobilization in the Wild and Scenic reach of the Rio Chama

This paper describes a study that deployed underwater acoustic sensors (hydrophones) to detect streambed mobilization in the Wild and Scenic reach of the Rio Chama, New Mexico. Fine sediment accumulation in the gravel‑cobble bed affects brown trout spawning habitat, and understanding incipient motion thresholds is essential for planning effective environmental flushing flows released from El Vado Dam. Two hydrophone stations were operated during 2021–2022, capturing audio data during a high‑flow pulse in late 2021. Acoustic processing techniques, including spectral analysis and threshold‑based impact detection, were used to identify sediment‑generated noise associated with coarse sediment transport. Results indicate detectable sediment mobilization during the high‑flow pulse, demonstrating hydrophones’ potential as a passive monitoring tool for environmental flow planning and sediment‑management decision support.

Conference Paper↗
USGS2026

Birds, breakpoints, and baselines: How citizen science data can reveal ecological boundaries in Kenya’s Upper Tana watershed

Tropical watersheds are increasingly threatened by climate change, land-use conversion, and resource extraction, yet conventional biodiversity monitoring in these systems is often spatially and temporally limited. Citizen science offers a complementary approach, enabling biodiversity data collection over large areas that can supplement professional scientific surveys. We analyzed 10 years (2012–2022) of Upper Tana Watershed bird data from the Kenya Bird Map project, covering 114 pentads (9 × 9 km) within a 17,000 km 2 watershed to assess patterns of bird community composition and distribution across this watershed and to also evaluate the effects of environmental variables, seasonality, and sampling effort to help inform improvements in future citizen science projects. Citizen (or community) scientists recorded 575 species (>50% of Kenya’s total avifauna) in 74 families. Asymptotic species accumulation indicates that most probable species present in the watershed were detected. Threshold indicator taxa analysis revealed distinct ecological boundaries along elevation (∼1,500 m), precipitation (∼1,100 mm), and mean temperature (∼19°C) gradients, corresponding to a turnover from xeric savanna to mesic montane forest assemblages. Notably, bird communities showed little seasonal differentiation between wet and dry periods, consistent with dominance by resident year-round species. Data limitations including uneven survey distribution and frequency, absence of abundance metrics, and coarse representation of local environmental conditions that likely reduced our ability to detect fine-scale species–habitat relationships. Addressing these gaps through spatially balanced sampling at greater resolution, greater survey frequency in underrepresented areas, and improved capture of habitat metrics could strengthen the use of citizen-science bird data for watershed bioassessment. Our findings demonstrate that structured citizen-science initiatives can identify ecological boundaries and inform adaptive management of tropical socio-ecological systems under rapid environmental change.

Upper Tana watershed↗
USGS2026

Multi-objective optimization of a hydro-economic model in an over-allocated agricultural basin

Groundwater depletion for agricultural irrigation poses significant environmental and economic challenges. This study introduces a proof-of-concept that combines hydro-economic modeling, scenario-based modeling, and multi-objective optimization to manage pumping curtailment in an over-allocated basin in the western United States. Three optimization scenarios were evaluated, each offering different degrees of management flexibility. Results reveal that scenarios with finer spatial resolution achieved greater environmental benefits per unit profit loss. Additionally, strategies allowing fractional reductions in curtailed wells–rather than complete shutdowns based on water rights seniority–substantially improved efficiency, highlighting the value of increased decision-making flexibility. Although scenario testing can aid stakeholder engagement and strategy exploration, multi-objective optimization provides a systematic framework to quantify tradeoffs between competing objectives. This combined approach demonstrates promise for building consensus and supporting the design of sustainable water management strategies that balance agricultural livelihoods with ecosystem preservation.

Oregon↗
USGS2026

Cultural benefits knowledge and social learning in Elwha River dam removal and ecosystem decision-making

In a retrospective case study of the seminal Elwha River dam removal in Washington State, USA, we explore pathways through which ecosystem services (ES) knowledge can inform environmental decision-making. We specifically investigate how knowledge of cultural benefits linked to the Elwha River ecosystem was made available. Through interviews and historical document review we identify diverse forms of cultural benefits knowledge (i.e., how individuals or groups know the contributions of an ecosystem to their identities, experiences, and capabilities), including both knowledge in the form of practices – embodied or enacted forms of knowledge, and knowledge in the form of products – quantitative or written documentation. Recognizing and learning from the diverse forms in which benefits knowledges are conveyed can support more accurate and equitable consideration of the diverse values, well-beings, and human-nature relationships linked to ecosystems. With this case study, we illuminate pathways for meaningful consideration of multiple knowledge forms, and the plural ecosystem values they convey, with relevance for environmental decision processes in the Puget Sound and beyond.

Washington↗
USGS2025

Evaluating mass flow meter measurements from chambers for greenhouse gas emissions from orphan wells and other point sources

This study evaluates the performance of a rigid gas flux chamber equipped with a mass flow meter (MFM) for measuring gas emissions from leaking orphan wells and similar pressure-driven gas point sources. We conducted a series of laboratory and field experiments to evaluate the sensitivity, stability, and dynamic range of an MFM chamber system and found an optimal method for sealing the chamber to the ground to isolate the emission source. From these results, we estimate the effects of different soil gas permeabilities on measurements and identify the uncertainty of environmental processes that can impact measurements. Simulations of an MFM chamber are compared to those of a dynamic flux chamber to contrast the data derived with both methodologies and illustrate the potential for measuring high variability leaks with the MFM chamber. Using a low flow resistance MFM and a chamber well-sealed to the ground, it is possible to measure leaks down to 1.08 x 10 -3 cubic meters per hour (m 3 h −1 ) (refenced to 25°/1 atm), corresponding to 0.77 grams per hour (g h −1 ) methane or 2.11 g h −1 carbon dioxide, with a mean uncertainty of 0.89 % relative standard deviation. Environmental processes such as heated gas inside the chamber from solar gain, wind blowing across the chamber vent, and changing humidity in the chamber, can cause variation in MFM measurements. Over 11 d of continuous monitoring under varying weather conditions, the standard deviation of the environmentally sourced signals was found to be 7.40 x 10 -3 m 3 h −1 (equivalent to or 5.27 g h −1 methane or 14.45 g h −1 carbon dioxide). Strategies to obtain the highest quality data from MFM chambers include burying the edges of the chamber below the surface sufficiently deep to seal the chamber edges against gas flow and soaking the dirt with water to lower the chances of escaping gases, while monitoring the gas flow and adjusting the chamber seal to achieve a maximum flow rate.

Atmospheric Measurement Techniques↗
USGS2025

Disturbance is the primary determinant of food chain length when the top predator is constant

Food chain length (FCL) is a primary determinant of food web structure and is hypothesized to be influenced by habitat size, productivity, and disturbance. Understanding the environmental characteristics that determine food chain length can assist in understanding how food webs may be impacted due to changes in habitats and environmental characteristics. This study examines the impact of hydrologic disturbance on stream food webs when the top predator is constant. We analyzed FCL in less disturbed groundwater flashy streams and more disturbed runoff flashy streams using stable isotopes. Despite no difference in species richness or fish density, food chains in more disturbed streams had a lower FCL compared to food chains in more stable streams. Assemblage analysis showed that flow regime and drainage area significantly impacted individual species abundances. The more disturbed runoff flashy streams had higher proportions of primary consumer fish, such as the algivorous Campostoma sp. (Stonerollers), which likely drives the reduced FCL. Drainage area and land cover had non-significant relationships with FCL. Shifting community structure due to hydrologic variability likely leads to differences in diet of Micropterus dolomieu (Smallmouth Bass), and thus a difference in FCL.

Arkansas, Missouri, Oklahoma↗
USGS2025

Component identification of solid biomass fuels using reflected light microscopy: Interlaboratory study 2

As nations transition toward sustainable energy systems , biomass has become a vital component of global energy portfolios. Derived from organic materials such as wood, agricultural residues, forestry byproducts, and organic waste, biomass is a renewable energy source with significant environmental and economic benefits. Responsible biomass energy production can improve waste management, reduce emissions of greenhouse gases, and mitigate environmental pollution. However, as the diversity of biomass-derived fuels increases, robust quality assessment methods are essential to ensure their efficiency, safety, and minimal environmental impact. Reflected light microscopy (RLM) is one such technique with the potential to complement conventional physico-chemical analyses by enabling a rapid identification of material constituents and impurities. To refine this methodology and evaluate the reproducibility of solid biomass component identification using RLM, an interlaboratory study (ILS) was conducted. The study involved the recognition of 58 components across 45 photomicrographs, with the participation of 65 scientists and students from 25 countries. The participants faced high difficulty identifying some of the marked components, and as a result, the percentage of correct answers ranged from 19.0 % to 98.3 %, with an average correct identification rate of 62.7 %. The most challenging aspects of the identification process included distinguishing between woody and non-woody (agro) biomass, accurately identifying petroleum-derived materials, and differentiating agro biomass from inorganic matter. The results suggest that while RLM is an important tool for characterizing solid biomass, further development of methodology guidelines and training are necessary to enhance its effectiveness. Future research should prioritize preparing detailed, image-rich, microscopic morphological descriptions of biomass fuel components, which could improve the accuracy and reliability of using RLM in biomass fuel characterization.

International Journal of Coal Geology↗
USGS2026

Decoding the oxidative digestion mechanism for polystyrene nanoplastic detection in the Great Lakes using a customizable Raman spectral processing algorithm

Despite the concerns surging around nanoplastics (NPs) regarding their prevalence and bioavailability in freshwater systems, robust detection of NPs in complex environmental matrices is hindered by the lack of standardized sample pretreatment and a mechanistic understanding of oxidative digestion. Here, we systematically investigate the interaction between hydrogen peroxide (H 2 O 2 ) and polystyrene (PS) NPs during digestion in deionized (DI) water and four environmental matrices from in and around the Great Lakes Basin. To facilitate high-throughput analysis, we develop Pre_peak, a customizable Raman spectral processing algorithm that achieves >99% accuracy for both NP identification and interference rejection, allowing reliable NP quantification via pixel counting and systematic decoding of the oxidative digestion mechanisms. In DI water, varying H 2 O 2 doses from 0 to 30% has negligible effects on the recovery and Raman signal intensity of PS NPs over 24 hours of digestion. However, morphological changes and aggregation of PS NPs are observed when the H 2 O 2 dose exceeds 20%. Prolonged digestion further leads to progressive NP loss. In natural waters, the optimal dosage and digestion duration depend on matrix characteristics, including dissolved organic matter (DOM) and ion composition. This study provides mechanistic insights into NP–oxidant interactions and underscores the need for matrix-tailored digestion protocols to advance standardized NP detection in freshwater environments.

Great Lakes↗
USGS2025

Shallow geologic framework of the Mississippi Sound and the potential for sediment resources

The Mississippi Sound, an estuarine environment located between the mainland and barrier islands bordering the northern Gulf of America (formerly the Gulf of Mexico), serves as a vital ecosystem for the States of Mississippi and Alabama. Spanning approximately 100 kilometers from east to west and covering 1,400 square kilometers, the sound is home to marine industry and ports, and its shallow and brackish waters sustain a diverse array of marine life. Barrier islands along the southern edge of the sound separate the microtidal estuary from the Gulf of America. This protection from gulf wave action mediates current flow within the sound, resulting in predominantly fine-grained sediment deposition along the seafloor. This study, conducted by the U.S. Geological Survey in cooperation with the U.S. Army Corps of Engineers, provides insight on fluvial and tidal processes spanning the past 5,000 years. The report synthesizes existing research to provide a comprehensive overview of the sound geology, from Pleistocene origins to present-day morphology, and utilizes high-resolution single channel seismic profiles and sediment data to identify and map sedimentary deposits and morphologic features at and below the seafloor. Despite its ecological significance, the Mississippi Sound faces environmental challenges, including water-quality issues, habitat degradation, storm-induced erosion, and the ongoing threats of sea-level rise and environmental changes. This study uses the present-day understanding of the sound's geology to inform coastal management decisions, hazard assessment, and potential mineral resources.

Louisiana, Mississippi↗
USGS2025

Review: The hydrogeology of critical mineral resources relevant to the energy transition

Attaining the goals of the international treaty on climate change (the Paris Agreement) will greatly increase the demand for the critical minerals required to implement clean-energy technologies. This poses both challenges and opportunities to the hydrogeologic community from several perspectives. Here, important insights that the hydrogeological sciences have to offer for mineral exploration, mineral production, and addressing environmental issues related to mining and mine decommissioning are summarized. This study focuses on copper, cobalt, lithium, and rare earths to represent the broad spectrum of critical minerals and illustrate their relevance by referring to projected demands and production rates. The current understanding of the hydrogeologic processes that form major deposits of these minerals are then summarized. Ore is defined as the naturally occurring material from which minerals of economic value can be extracted, where most ore deposits are the products of complex hydrogeologic couplings between fluid flow, heat transport, solute transport, chemical reactions, and mechanical deformation. Exploration models for the discovery of deeper, hidden deposits are potentially informed by hydrogeologic theory and hydrogeochemical processes. Hydrogeologic understanding and methods are also essential to production and recovery. Longstanding challenges are mine dewatering and (conversely) mine water supply, as well as mineral-extraction practices such as spoil heap leaching and in situ mining. New challenges arise from element extraction from subsurface brines. Finally, the quantity of water use and potential environmental impacts of mining on water quality are at the core of ‘social license’: the approval and acceptance of society to mining activities.

Hydrogeology Journal↗
USGS2021

Factors influencing distribution of Coccidioides immitis in soil, Washington State, 2016

Coccidioides immitis and Coccidioides posadasii are causative agents of Valley fever, a serious fungal disease endemic to regions with hot, arid climate in the United States, Mexico, and Central and South America. The environmental niche of Coccidioide s spp. is not well defined, and it remains unknown whether these fungi are primarily associated with rodents or grow as saprotrophs in soil. To better understand the environmental reservoir of these pathogens, we used a systematic soil sampling approach, quantitative PCR (qPCR), culture, whole-genome sequencing, and soil chemical analysis to identify factors associated with the presence of C. immitis at a known colonization site in Washington State linked to a human case in 2010. We found that the same strain colonized an area of over 46,000 m 2 and persisted in soil for over 6 years. No association with rodent burrows was observed, as C. immitis DNA was as likely to be detected inside rodent holes as it was in the surrounding soil. In addition, the presence of C. immitis DNA in soil was correlated with elevated levels of boron, calcium, magnesium, sodium, and silicon in soil leachates. We also observed differences in the microbial communities between C. immitis -positive and -negative soils. Our artificial soil inoculation experiments demonstrated that C. immitis can use soil as a sole source of nutrients. Taken together, these results suggest that soil parameters need to be considered when modeling the distribution of this fungus in the environment.

Washington↗