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Problems with the claim of ecotype and taxon status of the wolf in the Great Lakes region

Koblmuller et al. (2009) analysed molecular genetic data of the wolf in the Great Lakes (GL) region of the USA and concluded that the animal was a unique ecotype of grey wolf and that genetic data supported the population as a discrete wolf taxon. However, some of the literature that the researchers used to support their position actually did not, and additional confusion arises from indefinite use of terminology. Herein, we discuss the problems with designation of a wolf population as a taxon or ecotype without proper definition and assessment of criteria.

Great Lakes Region

Tracking animals in freshwater with electronic tags: past, present and future

Considerable technical developments over the past half century have enabled widespread application of electronic tags to the study of animals in the wild, including in freshwater environments. We review the constraints associated with freshwater telemetry and biologging and the technical developments relevant to their use. Technical constraints for tracking animals are often influenced by the characteristics of the animals being studied and the environment they inhabit. Collectively, they influence which and how technologies can be used and their relative effectiveness. Although radio telemetry has historically been the most commonly used technology in freshwater, passive integrated transponder (PIT) technology, acoustic telemetry and biologgers are becoming more popular. Most telemetry studies have focused on fish, although an increasing number have focused on other taxa, such as turtles, crustaceans and molluscs. Key technical developments for freshwater systems include: miniaturization of tags for tracking small-size life stages and species, fixed stations and coded tags for tracking large samples of animals over long distances and large temporal scales, inexpensive PIT systems that enable mass tagging to yield population- and community-level relevant sample sizes, incorporation of sensors into electronic tags, validation of tag attachment procedures with a focus on maintaining animal welfare, incorporation of different techniques (for example, genetics, stable isotopes) and peripheral technologies (for example, geographic information systems, hydroacoustics), development of novel analytical techniques, and extensive international collaboration. Innovations are still needed in tag miniaturization, data analysis and visualization, and in tracking animals over larger spatial scales (for example, pelagic areas of lakes) and in challenging environments (for example, large dynamic floodplain systems, under ice). There seems to be a particular need for adapting various global positioning system and satellite tagging approaches to freshwater. Electronic tagging provides a mechanism to collect detailed information from imperilled animals and species that have no direct economic value. Current and future advances will continue to improve our knowledge of the natural history of aquatic animals and ecological processes in freshwater ecosystems while facilitating evidence-based resource management and conservation.

Animal Biotelemetry

Feral horse space use and genetic characteristics from fecal DNA

Feral horses ( Equus ferus caballus ) in the western United States are managed by the Bureau of Land Management (BLM) and United States Forest Service in designated areas on public lands with a goal of maintaining populations in balance with multiple uses of the landscape. Small, isolated populations can be at risk of extirpation from stochastic events and deleterious genetic effects resulting from inbreeding and reduced heterozygosity. The genetic diversity of feral horse herds is periodically monitored using blood or hair samples collected during management gathers (i.e., occasions when the herd is rounded up). We conducted a study to examine genetic characteristics of the feral horse population at the BLM Little Book Cliffs Herd Management Area (HMA) in Colorado, USA, using non-invasively collected fecal samples. Additionally, we explored whether genotypes could be used to document space use and potential sub-population development. We used a random sampling scheme, walking transects in sampling areas covering most of the HMA to find and collect fecal samples of all ages, except those that were deteriorating. We collected >1,800 fecal samples from across the study area in May, August, and October 2014. We then identified unique individuals using a suite of microsatellite loci. Our estimates of genetic diversity from fecal samples were higher than those reported from blood and hair samples taken during recent horse gathers, likely because our sample size and spatial distribution was larger. Genotypes revealed that some individuals were found only in certain parts of the study area and at a higher proportion than random; thus, they could be considered residents in those sampling areas. Using discriminant function analyses, we detected 5 genetic groups in the sample population, but these did not correspond to individuals in specific parts of the study area. Our results support the use of fecal DNA to augment direct observations of horse presence and could be used to detect habitat use and areas of high density. Non-invasive techniques such as fecal DNA sampling can help managers decide whether new individuals need to be translocated to a closed population to maintain genetic diversity without the human safety and animal welfare concerns associated with gathers and invasive techniques.

Colorado

Elevated temperature exacerbates Ichthyophonus infections in buffalo sculpin

High incidences of Ichthyophonus hoferi, a parasite primarily of marine and estuarine fishes, have recently been reported in rockfishes and Pacific herring from the eastern North Pacific (Kent et al 2001, Hershberger et al 2002). Taxonomic position of I. hoferi remains unresolved, but recent phylogenetic studies have grouped the organism with Dermocystidium, Psorospermium, the rosette agent of salmonids, and Rhinosporidium in the Mesomycetozoa, a novel clade of protists near the animal-fungal divergence (Herr et al 1999). Genetic differences among isolates from the east coast of North America indicate that more than one species of Ichthyophonus exist (Rand et al 2000), and different species have likely been assigned the same name based on morphological characteristics. Therefore, hereafter in this manuscript, the organism will be referred to as Ichthyophonus .

Fish Health Newsletter

Rapid growth and genetic diversity retention in an isolated reintroduced black bear population in the central appalachians

Animal reintroductions are important tools of wildlife management to restore species to their historical range, and they can also create unique opportunities to study population dynamics and genetics from founder events. We used non-invasive hair sampling in a systematic, closed-population capture-mark-recapture (CMR) study design at the Big South Fork (BSF) area in Kentucky during 2010 and Tennessee during 2012 to estimate the demographic and genetic characteristics of the black bear ( Ursus americanus ) population that resulted from a reintroduced founding population of 18 bears in 1998. We estimated 38 (95% CI: 31–66) and 190 (95% CI: 170–219) bears on the Kentucky and Tennessee study areas, respectively. Based on the Tennessee abundance estimate alone, the mean annual growth rate was 18.3% (95% CI: 17.4–19.5%) from 1998 to 2012. We also compared the genetic characteristics of bears sampled during 2010–2012 to bears in the population during 2000–2002, 2–4 years following reintroduction, and to the source population. We found that the level of genetic diversity since reintroduction as indicated by expected heterozygosity ( H E ) remained relatively constant ( H E(source, 2004)  = 0.763, H E(BSF, 2000–2002)  = 0.729, H E(BSF, 2010–2012)  = 0.712) and the effective number of breeders ( N B ) remained low but had increased since reintroduction in the absence of sufficient immigration ( N B(BSF, 2000–2002)  = 12, N B(BSF, 2010–2012)   = 35). This bear population appears to be genetically isolated, but contrary to our expectations, we did not find evidence of genetic diversity loss or other deleterious genetic effects typically observed from small founder groups. We attribute that to high initial genetic diversity in the founder group combined with overlapping generations and rapid population growth. Although the population remains relatively small, the reintroduction using a small founder group appears to be demographically and genetically sustainable.

Kentucky, Tennessee

Yellowstone bison genetics: let us move forward

White and Wallen (2012) disagree with the conclusions and suggestions made in our recent assessment of population structure among Yellowstone National Park (YNP) bison based on 46 autosomal microsatellite loci in 661 animals (Halbert et al. 2012). First, they suggest that "the existing genetic substructure (that we observed) was artificially created." Specifically, they suggest that the substructure observed between the northern and central populations is the result of human activities, both historical and recent. In fact, the genetic composition of all known existing bison herds was created by, or has been influenced by, anthropogenic activities, although this obviously does not reduce the value of these herds for genetic conservation (Dratch and Gogan 2010). As perspective, many, if not most, species of conservation concern have been influenced by human actions and as a result currently exist as isolated populations. However, it is quite difficult to distinguish between genetic differences caused by human actions and important ancestral variation contained in separate populations without data from early time periods. Therefore, to not lose genetic variation that may be significant or indicative of important genetic variation, the generally acceptable management approach is to attempt to retain this variation based on the observed population genetic subdivision (Hedrick et al. 1986).

Yellowstone National Park

A new DNA extraction method (HV-CTAB-PCI) for amplification of nuclear markers from open ocean-retrieved faeces of an herbivorous marine mammal, the dugong

Non-invasively collected faecal samples are an alternative source of DNA to tissue samples, that may be used in genetic studies of wildlife when direct sampling of animals is difficult. Although several faecal DNA extraction methods exist, their efficacy varies between species. Previous attempts to amplify mitochondrial DNA (mtDNA) markers from faeces of wild dugongs ( Dugong dugon ) have met with limited success and nuclear markers (microsatellites) have been unsuccessful. This study aimed to establish a tool for sampling both mtDNA and nuclear DNA (nDNA) from dugong faeces by modifying approaches used in studies of other large herbivores. First, a streamlined, cost-effective DNA extraction method that enabled the amplification of both mitochondrial and nuclear markers from large quantities of dugong faeces was developed. Faecal DNA extracted using a new ‘High Volume- Cetyltrimethyl Ammonium Bromide- Phenol-Chloroform-Isoamyl Alcohol’ (HV-CTAB-PCI) method was found to achieve comparable amplification results to extraction of DNA from dugong skin. As most prevailing practices advocate sampling from the outer surface of a stool to maximise capture of sloughed intestinal cells, this study compared amplification success of mtDNA between the outer and inner layers of faeces, but no difference in amplification was found. Assessment of the impacts of faecal age or degradation on extraction, however, demonstrated that fresher faeces with shorter duration of environmental (seawater) exposure amplified both markers better than eroded scats. Using the HV-CTAB-PCI method, nuclear markers were successfully amplified for the first time from dugong faeces. The successful amplification of single nucleotide polymorphism (SNP) markers represents a proof-of-concept showing that DNA from dugong faeces can potentially be utilised in population genetic studies. This novel DNA extraction protocol offers a new tool that will facilitate genetic studies of dugongs and other large and cryptic marine herbivores in remote locations.

Queensland

Low occurrence of multi-antimicrobial and heavy metal resistance in Salmonella enterica from wild birds in the United States

Wild birds are common reservoirs of Salmonella enterica . Wild birds carrying resistant S . enterica may pose a risk to public health as they can spread the resistant bacteria across large spatial scales within a short time. Here, we whole-genome sequenced 375 S . enterica strains from wild birds collected in 41 U.S. states during 1978–2019 to examine bacterial resistance to antibiotics and heavy metals. We found that Typhimurium was the dominant S . enterica serovar, accounting for 68.3% (256/375) of the bird isolates. Furthermore, the proportions of the isolates identified as multi-antimicrobial resistant (multi-AMR: resistant to at least three antimicrobial classes) or multi-heavy metal resistant (multi-HMR: resistant to at least three heavy metals) were both 1.87% (7/375). Interestingly, all the multi-resistant S . enterica ( n = 12) were isolated from water birds or raptors; none of them was isolated from songbirds. Plasmid profiling demonstrated that 75% (9/12) of the multi-resistant strains carried resistance plasmids. Our study indicates that wild birds do not serve as important reservoirs of multi-resistant S . enterica strains. Nonetheless, continuous surveillance for bacterial resistance in wild birds is necessary because the multi-resistant isolates identified in this study also showed close genetic relatedness with those from humans and domestic animals.

Environmental Microbiology

How accurately does eDNA reflect the spatial distribution of cold-water fish? Field validation from a temperate lake

Applications of environmental DNA (eDNA) based detection technology to evaluate the distribution of aquatic organisms are increasing; yet field validations of eDNA are important to measure accuracy in study systems. To successfully apply this technology to species conservation, it is critical to understand how both species biology and environmental conditions affect the accuracy of inference from eDNA detection data. We implemented a field assessment of the accuracy and spatial resolution of eDNA-based species distributions for a native cold-water, schooling fish, cisco Coregonus artedi , that has been reintroduced to a deep temperate lake. We leveraged a combination of acoustic telemetry, providing known spatial locations of tagged fish, and lake-wide eDNA sampling to infer their distribution in Keuka Lake, New York, USA. Sub-surface (12 m and 18 m depths) eDNA samples were collected to accommodate the diel vertical migration behaviour of this fish species. The results of this study validated the accuracy of positive eDNA detections with the distribution of tagged fish to coarse spatial scales. Yet, several fine-scale locations revealed a mismatch between eDNA and acoustic telemetry detections; consistent with rapid transport of genetic material via lake currents. Empirical measurements of lake currents using drifters found cisco eDNA detections could deviate from specimens' source locations by as much as 3.3 km at 12 m depth or 1.5 km at 18 m depth over a 24 h transport period. Our study indicates that accurate species distributions estimated from eDNA sampling in lakes may require further understanding of transport mechanisms and persistence of environmental genetic material to relate point detections to source animal locations. Integrating eDNA sampling with additional data collection of species biology and environmental conditions will increase the spatial resolution of fish distribution assessments.

New York

Research to support sterile-male-release and genetic alteration techniques for sea lamprey control

Integrated pest management of sea lampreys in the Laurentian Great Lakes has recently been enhanced by addition of a sterile-male-release program, and future developments in genetic approaches may lead to additional methods for reducing sea lamprey reproduction. We review the development, implementation, and evaluation of the sterile-male-release technique (SMRT) as it is being applied against sea lampreys in the Great Lakes, review the current understanding of SMRT efficacy, and identify additional research areas and topics that would increase either the efficacy of the SMRT or expand its geographic potential for application. Key areas for additional research are in the sterilization process, effects of skewed sex ratios on mating behavior, enhancing attractiveness of sterilized males, techniques for genetic alteration of sea lampreys, and sources of animals to enhance or expand the use of sterile lampreys.

Journal of Great Lakes Research

An animal location-based habitat suitability model for bighorn sheep and wild horses in Bighorn Canyon National Recreation Area and the Pryor Mountain Wild Horse Range, Montana, and Wyoming

The purpose of this habitat suitability model is to provide a tool that will help managers and researchers better manage bighorn sheep and wild horses in the Bighorn Canyon National Recreation Area (BICA) and Pryor Mountain Wild Horse Range (PMWHR). A concern in the management of the Pryor Mountain wild horse population is whether or not the wild horses compete with bighorn sheep for available forage or available space. Two studies have been conducted that have shown no obvious, convincing competition between the two species. A study of diets and habitat-use of both species revealed substantial diet overlap only during some seasons, but there were considerable spatial and habitat separations between wild horses and bighorns during all seasons (Kissell and others, 1996). This empirical data was then used in a modeling exercise that predicted that neither the current (about 160 horses at the time of the analysis) nor larger numbers of wild horses on the area (e.g., about 200 horses) would result in reduced numbers or condition of bighorn sheep (Coughenour 1999). But competition is a very complex biological process to document. Bighorns might have already been spatially avoiding wild horses when these studies were conducted. A second concern for managers is that earlier studies suggest both species are not using many areas of the range that appear to be suitable (Gudorf and others, 1996; Kissell and others, 1996). A primary goal for the management of both species is to increase their numbers for purposes of genetic conservation and viability. The bighorn sheep population declined during the mid-1990’s from a peak of about 211 animals to ~ 100 animals at present. Absolute minimum goals for genetic viability in the bighorn sheep herd (genetic effective population size of N >50) suggest at least 150 animals should be present, while studies of persistence suggest populations of 250+ are e more likely to recover rapidly and persist should the population experience an epizootic die-off (Singer and others, 2001). Since all bighorn sheep populations are potentially vulnerable to disease epizootics, managing for larger populations of 200–300 animals appears to increase the potential for long-term persistence (Berger, 1990; Singer and others, 2001). Wild horses are not prone to rapid disease die-offs. However, minimum goals for genetic viability in the Pryor Mountain wild horses ( Ne > 50) require that at least 160 animals be present on the range (Singer and others, 2000). Since the Ne > 50 goal is set for the breeding of domestic animals, and since the vagaries of drought, severe winters, predation, and other stochastic events cause stress in wild animals, larger goals for Ne (e.g. Ne > 100) for wild horses are even more desirable (USDI, BLM, 1999; Gross, 2000). Expanding the area of the wild horse range is one option, but the prospects for expanding the range do not appear to be great (L. Coates-Markle, BLM, oral comm.). A second option would be to increase the amount of useable habitat for horses on the existing range. One goal of this modeling effort was to use GIS-based habitat analyses to determine the reason wild horses are not using some areas of the range, and to explore the potential for making some of these areas useable. The National Park Service (NPS) has shown considerable interest in management actions within BICA that will increase the range, useable habitat, and population size of bighorn sheep. There has also been interest expressed by the Bureau of Land Management (BLM) and wild horse advocates to improve the useable habitat for wild horses and to possibly increase the size of the horse range.

Report

Using science to inform management and improve biological conservation in the Desert Renewable Energy Conservation Plan

The Mojave and Colorado deserts of southern California have been viewed as vast wilderness since early exploration and, until recently, were considered the most untrammeled among western landscapes in the contiguous lower 48 states (United States Department of Agriculture 1893; Leu et al. 2008). However, the factors that define desert wilderness—small human population, temperature differentials that create unrelenting winds, low rainfall, and cloudless skies—are attractive for renewable energy development. The demand for clean, renewable energy is a national and regional priority and has increased demand for large-scale solar and wind farms in the deserts, particularly in California. The need to balance these national and state energy priorities with existing natural resource and land conservation policies has emerged as a landscape-scale land-use planning initiative known as the Desert Renewable Energy Conservation Plan (DRECP). One of the primary goals for the DRECP was to establish Development Focus Areas (DFAs) where high-quality renewable energy potential of up to 20 gigawatts (GW) could be implemented by the year 2040. DFAs were designed to provide expedited project approvals in locations where environmental impacts could be managed and mitigated, and proximity to transmission corridors provides for the efficient dissemination of energy to users. The DRECP also aims to identify protections for natural resources, recreation, and cultural resources. This plan identifies 37 covered species that receive special consideration in the DRECP. Among the special considerations are climate adaptation requirements, such as the ability to maintain population connectivity through wildlife corridors, while protecting several special recreation areas and 32,000 known cultural sites that are dispersed throughout the region. We evaluated several particular aspects of the DRECP design and process. In particular, we examined land designations in relation to published studies on Mohave ground squirrel (Xerospermophilus mohavensis) habitat and genetic diversity patterns of a suite of broadly distributed desert animal species. The squirrel and its habitat are of particular interest because the entire range of the squirrel is encompassed by the DRECP. We describe the framework of the DRECP, provide a case study of how the DRECP accommodates the needs of the Mohave ground squirrel and its habitat, illustrate DFAs in relation to the genetic diversity of a broad range of terrestrial biota, and conclude with some observations on how land-use issues were resolved across the landscape under various scenarios.

Conference Paper

A complex alloantigen system in Florida sandhill cranes, Grus canadensis pratensis: Evidence for the major histocompatibility (B) system

The B blood group system constitutes the major histocompatibility complex ( Mhc ) in birds. The Mhc is a cluster of genes largely devoted to the processing and presentation of antigen. The Mhc is highly polymorphic in many species and, thus, useful in the evaluation of genetic diversity for fitness traits within populations of a variety of animals. Correlations found between particular Mhc haplotypes and resistance to certain diseases emphasize the importance of understanding the functional significance of diversity of the Mhc , particularly in species threatened with extinction. As part of studies focused on genetic diversity in wild birds, serological techniques were used to define a highly polymorphic alloantigen system in seven families of Florida sandhill cranes ( Grus canadensis pratensis ). The results of analyses with antisera produced within the crane families and with chicken Mhc antigen-specific reagents revealed a single major alloantigen system that is likely the Mhc of the Florida sandhill crane. Preliminary experiments indicate that these crane alloantisera will provide a means of defining the Mhc in other species of cranes.

Journal of Heredity

High dispersal rates in hybrids drive expansion of maladaptive hybridization

Hybridization between native and invasive species, a major cause of biodiversity loss, can spread rapidly even when hybrids have reduced fitness. This paradox suggests that hybrids have greater dispersal rates than non-hybridized individuals, yet this mechanism has not been empirically tested in animal populations. Here, we test if non-native genetic introgression increases reproductive dispersal using a human-mediated hybrid zone between native cutthroat trout ( Oncorhynchus clarkii ) and invasive rainbow trout ( Oncorhynchus mykiss ) in a large and connected river system. We quantified the propensity for individuals to migrate from natal rearing habitats (migrate), reproduce in non-natal habitats (stray), and the joint probability of dispersal as a function of genetic ancestry. Hybrid trout with predominantly non-native rainbow trout ancestry were more likely to migrate as juveniles and to stray as adults. Overall, hybrids with greater than 50% rainbow trout ancestry were 5.7 times more likely to disperse than native or hybrid trout with small amounts of rainbow trout ancestry. Our results show a genetic basis for increased dispersal in hybrids that is likely contributing to the rapid expansion of invasive hybridization between these species. Management actions that decrease the probability of hybrid dispersal may mitigate the harmful effects of invasive hybridization on native biodiversity.

Montana

Use of noninvasive genetics to assess nest and space use by white-tailed eagles

Movement and space use are important components of animal interactions with the environment. However, for hard-to-monitor raptor species, there are substantial gaps in our understanding of these key determinants. We used noninvasive genetic tools to evaluate the details of space use over a 3-yr period by White-tailed Eagles ( Haliaeetus albicilla ) at the Naurzum Zapovednik in northern Kazakhstan. We genotyped, at 10 microsatellite markers and one mitochondrial marker, 859 eagle feathers and assigned naturally shed feathers to individuals. We identified 124 White-tailed Eagles, including both members of 5–10 pairs per year, and were able to monitor birds across years. Distances between eagle nests and hunting perches were always greater than nearest neighbor distances, eagles never used the closest available hunting perch, and hunting perches were always shared with other eagles. When eagles switched nests between years, the nests they chose were almost always well outside the space that theory predicted they defended the prior year. Our data are inconsistent with classical territorial and colonial models of resource use; they more closely resemble semi-colonial behavior. It is unlikely that standard methods of animal tracking (e.g., marking and telemetry), would have provided a similarly cost-effective mechanism to gain these insights into spatial and temporal aspects of eagle behavior. When combined with existing information on space use of other local species, these data suggest that partitioning of spatial resources among White-tailed Eagles and other eagles at the Zapovednik may be facilitated by the alternative strategies of space use they employ.

Naurzum Zapovednik

Flow cytometry used to assess genetic damage in frogs from farm ponds

Flow cytometry (FC) is a laboratory method used to detect genetic damage induced by environmental contaminants and other stressors in animals, including amphibians. We tested FC methods on three species of ranid frogs collected from farm ponds and natural wetlands in southeastern Minnesota. We compared FC metrics for Rana clamitans between ponds with direct exposure to agricultural contaminants and reference (unexposed) ponds. Concentrations of atrazine in water from our farm ponds ranged from 0.04 to 0.55 ppb. We found that R. clamitans from exposed ponds had DNA content similar to frogs from unexposed ponds. Pond-averaged C-values (a measure of DNA content) ranged from 6.53 to 7.08 for R. pipiens (n . 13), 6.55 to 6.60 for R. clamitans (n . 40) and 6.74 for R. palustris (n . 5). Among all species, the mean sample CVs ranged from 1.91 (R. palustris) to 6.31 (R. pipiens). Deformities were observed in only 2 of 796 individuals among all species and occurred in both reference and exposed ponds. Although we did not detect evidence of DNA damage associated with agriculture in our study, we demonstrated the potential of FC for screening amphibian populations for genetic damage. Metrics from a variety of amphibian species and locations as well as laboratory studies are needed to further assess the value of FC for monitoring amphibian genetic integrity in contaminated sites.

Journal of the Iowa Academy of Science

Genetic characterisation of Toxoplasma gondii in wildlife from North America revealed widespread and high prevalence of the fourth clonal type

Little is known of the genetic diversity of Toxoplasma gondii circulating in wildlife. In the present study wild animals, from the USA were examined for T. gondii infection. Tissues of naturally exposed animals were bioassayed in mice for isolation of viable parasites. Viable T. gondii was isolated from 31 animals including, to our knowledge for the first time, from a bald eagle ( Haliaeetus leucocephalus ), five gray wolves ( Canis lupus ), a woodrat ( Neotoma micropus) , and five Arctic foxes (Alopex lagopus) . Additionally, 66 T. gondii isolates obtained previously, but not genetically characterised, were revived in mice. Toxoplasma gondii DNA isolated from these 97 samples (31 + 66) was characterised using 11 PCR-restriction fragment length polymorphism (RFLP) markers (SAG1, 5′- and 3′-SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22–8, c29–2, L358, PK1 and Apico). A total of 95 isolates were successfully genotyped. In addition to clonal Types II, and III, 12 different genotypes were found. These genotype data were combined with 74 T. gondii isolates previously characterised from wildlife from North America and a composite data set of 169 isolates comprised 22 genotypes, including clonal Types II, III and 20 atypical genotypes. Phylogenetic network analysis showed limited diversity with dominance of a recently designated fourth clonal type (Type 12) in North America, followed by the Type II and III lineages. These three major lineages together accounted for 85% of strains in North America. The Type 12 lineage includes previously identified Type A and X strains from sea otters. This study revealed that the Type 12 lineage accounts for 46.7% (79/169) of isolates and is dominant in wildlife of North America. No clonal Type I strain was identified among these wildlife isolates. These results suggest that T. gondii strains in wildlife from North America have limited diversity, with the occurrence of only a few major clonal types.

International Journal for Parasitology

Book review: Behavioral ecology of the eastern red-backed salamander: 50 years of research

In commemoration of the 100th anniversary of the British Ecological Society, Sutherland et al. (2013) identified 100 questions of fundamental significance in “pure” (i.e., not applied) ecology. A somewhat unexpected outcome of these authors’ exercise was the realization that, after 100 years of comprehensive, intensive scientific research, there remained “profound knowledge gaps” in ecology, such as a clear understanding of “the central mechanisms driving ecosystems…communities…, and even population dynamics.” Animal behavior (along with other attributes such as physiology and genetics) is such a mechanism that can structure ecological interactions, and the study of behavioral ecology provides important insights into many fundamental ecological phenomena. For example, the well-known historical characterization of ecology as the study of the distribution and abundance of organisms (Andrewartha and Birch 1954) invokes numerous questions, such as: what factors influence coexistence among competing species, or between predators and their prey? Ultimately, the answers to these and other questions are best addressed with fine-scale, mechanistic studies of habitat selection, foraging behavior/prey selection, and movement/dispersal behavior. Similarly, at the population level, insight into the spatial distribution of individuals could be gained with studies of territoriality, dominance hierarchies, and even mate choice.

Herpetological Review