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Investigation of fish communities in natural channel sections of the Mohawk River, New York

Little is known about the natural resources in the natural channel sections of the Mohawk River between Rome and Frankfort, New York, where the river channel runs separately from and parallel to the Erie Canal. This river section runs through multiple locations that are listed as active remediation sites under New York’s Inactive Hazardous Waste Disposal Site Program and has negligible public or commercial access. As a result, there is minimal recreational usage of this river section, and efforts to conduct biological sampling have been limited. To better understand the composition of fish communities and contaminant concentrations in the natural channel of the Mohawk River, the U.S. Geological Survey, in cooperation with the New York State Department of Environmental Conservation, sampled resident fish in the 34-kilometer section from the mouth of Oriskany Creek downstream to Frankfort. Fish communities were sampled using boat electrofishing during 2021, 2023, and 2024 across multiple reaches within this section. These data were used to estimate species richness (number of species), relative abundance and biomass (catch rates), population size structure (distribution of lengths), and fish condition (a proxy for health). Some gamefish specimens were also analyzed to determine contaminant concentrations in fish tissue. A total of 1,531 individual fish and 38 fish species were captured across all surveys. Seventeen of the 38 (45 percent) species were classified as native to the Mohawk River watershed, whereas 21 of the 38 (55 percent) species were classified as nonnative. Some popular gamefish species such as largemouth bass ( Micropterus salmoides ) and smallmouth bass ( Micropterus dolomieu ) were abundant in most reaches, whereas others such as walleye ( Sander vitreus ) and northern pike ( Esox lucius ) were found sporadically. Only one round goby ( Neogobius melanostomus ) was captured, indicating that this high-profile invasive species remains uncommon in this habitat. A backwater reach had the greatest relative abundance and condition of many species. This indicates connected lentic habitats such as oxbows and backwaters may be important nursery and refuge areas in the aquatic ecosystem. Polychlorinated biphenyl (PCB) concentrations in fish tissue were highly elevated relative to the New York State Department of Health (NYSDOH) fish consumption “don’t eat” advisory guideline. Some specimens exceeded the guideline by an order of magnitude or more, particularly those from a localized area downstream from the Utica Harbor Dam. Concentrations of perfluorooctanesulfonic acid (PFOS) often approached the NYSDOH “don’t eat” consumption guideline but only one sample exceeded the guideline. Concentrations of other contaminants such as mercury and pesticides were consistently measured at less than the “don’t eat” consumption guidelines. These data indicate PCBs remain the primary contaminant of concern in the natural channel of the Mohawk River and are readily bioaccumulating in aquatic organisms despite prior remediation measures. Taken together, the findings in this report are intended to inform future decisions related to fisheries management, public access, recreational usage, and fish consumption advisories.

New York

Geology and ground-water resources of the Ahtanum Valley, Yakima County, Washington

The Ahtanum Valley covers an area of about 100 square miles in an important agricultural district in central Yakima County, Wash. Because the area is semiarid, virtually all crops require irrigation. Surface-water supplies are inadequate in most of the area, and ground water is being used increasingly for irrigation. The purpose of this investigation was the collection and interpretation of data, pertaining to ground water in the area as an aid in the proper development and management of the water resources. The occurrence and movement of ground water in the Ahtanum Valley are directly related to the geology. The valley occupies part of a structural trough (Ahtanum-Moxee subbasin) that is underlain by strongly folded flow layers of a thick sequence of the Yakima basalt. The upper part of the basalt sequence interfingers with, and is conformably overlying by, sedimentary rocks of the Ellensburg formation which are as much as 1,000 feet thick. These rocks are in turn overlying unconformably by cemented basalt gravel as much as 400 feet thick. Unconsolidated alluvial sand and gravel, as much as 30 feet thick, form the valley floor. Although ground water occurs in each of the rock units within the area, the Yakima basalt and the unconsolidated alluvium yield about three-fourths of the ground water currently used. Wells in the area range in depth from a few feet to more than 1,200 feet and yield from less than 1 to more than 1,030 gallons per minute. Although water levels in water-table wells usually are shallow--often less than 5 feet below the land surface--levels in deeper wells tapping confined water range from somewhat above the land surface (in flowing wells) to about 200 feet below. Wells drilled into aquifers in the Yakima basalt, the Ellensburg formation, and the cemented gravel usually tap confined water, and at least 12 wells in the area flow or have flowed in the past. Ground-water levels fluctuate principally in response to changes in stream levels, variations in the flow of irrigation ditches and in rates of water application, variations in local precipitation, and seasonal differences in withdrawals from wells. Annual fluctuations of levels generally are less than 10 feet except in localities of heavy pumping. Periodic measurements of water levels in two observation wells in the area indicate, locally at least, a persistent decline in artesian pressures in confined basalt aquifers, although the record is too short to show whether withdrawal by pumping has reached, or is nearing, an optimum balance with recharge. The aquifers are recharged by precipitation, by infiltration from streams, and by ground-water underflow into the area. Ground water is discharged by seepage to streams, by evapotranspiration, by springs and seeps at the land surface, and, artificially, by withdrawal from wells. It is estimated that the seepage discharge to the Yakima River from the area studied may range from about 20,000 to 25,000 acre-feet per year. The consumptive waste of ground water by phreatophytes probably exceeds 4,000 acre-feet per year and may represent a large reclaimable source of water in the area. The annual withdrawal of ground water from wells in the area for domestic, industrial, irrigation, public, and stock supplies is estimated to be 6,300 acre-feet. The chemical quality of the ground water generally is satisfactory for most purposes, although the water from many wells is harder than is desirable for domestic use.

Washington

Landscape features fail to explain spatial genetic structure in white-tailed deer across Ohio, USA

Landscape features influence wildlife movements across spatial scales and have the potential to influence the spread of disease. Chronic wasting disease (CWD) is a fatal prion disease affecting members of the family Cervidae, particularly white-tailed deer ( Odocoileus virginianus ), and the first positive CWD case in a wild deer in Ohio, USA, was recorded in 2020. Landscape genetics approaches are increasingly used to better understand potential pathways for CWD spread in white-tailed deer, but little is known about genetic structure of white-tailed deer in Ohio. The objectives of our study were to evaluate spatial genetic structure in white-tailed deer across Ohio and compare the support for isolation by distance (IBD) and isolation by landscape resistance (IBR) models in explaining this structure. We collected genetic data from 619 individual deer from 24 counties across Ohio during 2007–2009. We used microsatellite genotypes from 619 individuals genotyped at 11 loci and haplotypes from a 547-base pair fragment of the mitochondrial DNA control region. We used spatial and non-spatial genetic clustering tests to evaluate genetic structure in both types of genetic data and empirically optimized landscape resistance surfaces to compare IBD and IBR using microsatellite data. Non-spatial genetic clustering tests failed to detect spatial genetic structure, whereas spatial genetic clustering tests indicated subtle spatial genetic structure. The IBD model consistently outperformed IBR models that included land cover, traffic volume, and streams. Our results indicated widespread genetic connectivity of white-tailed deer across Ohio and negligible effects of landscape features. These patterns likely reflect some combination of minimal resistive effects of landscape features on white-tail deer movement in Ohio and the effects of regional recolonization or translocation. We encourage continued CWD surveillance in Ohio, particularly in the proximity of confirmed cases.

Ohio

Potential utility of environmental DNA for early detection of Eurasian watermilfoil ( Myriophyllum spicatum )

Considering the harmful and irreversible consequences of many biological invasions, early detection of an invasive species is an important step toward protecting ecosystems (Sepulveda et al. 2012). Early detection increases the probability that suppression or eradication efforts will be successful because invasive populations are small and localized (Vander Zanden et al. 2010). However, most invasive species are not detected early because current tools have low detection probabilities when target species are rare and the sampling effort required to achieve acceptable detection capabilities with current tools is seldom tractable (Jerde et al. 2011). As a result, many invasive species go undetected until they are abundant and suppression efforts become costly. Novel DNA-based surveillance tools have recently revolutionized early detection abilities using environmental DNA (eDNA) present in the water (Darling and Mahon 2011, Bohmann et al. 2014). In brief, eDNA monitoring enables the identification of organisms from DNA present and collected in water samples. Aquatic and semiaquatic organisms release DNA contained in sloughed, damaged, or partially decomposed tissue and waste products into the water and molecular techniques allow this eDNA in the water column to be identified from simple and easy-tocollect water samples (Darling and Mahon 2011). Despite limited understanding of the production, persistence, and spread of DNA in water (Barnes et al. 2014), eDNA monitoring has been applied not only to invasive species (Jerde et al. 2011), but also to species that are rare, endangered, or highly elusive (Spear et al. 2014). However, most eDNA research and monitoring has focused on detection of invertebrates and vertebrates and less attentionhas been given to developing eDNA techniques for detecting aquatic invasive plants. Eurasian watermilfoil (EWM; Myriophyllum spicatum L.) is an invasive species for which improved early detection would be particularly helpful. Advanced EWM invasions have negative impacts on native biodiversity, recreational boating, fishing, and other types of aquatic tourism (e.g., Eiswerth et al. 2000). On a broader scale, EWM can also be harmful to man-made aquatic infrastructure, such as hydroelectric dams. If an EWM invasion can be detected in an early stage where eradication is still a possibility, many of these negative consequences can be limited or prevented altogether (e.g., Madsen et al. 2002). The purpose of this research was to develop and validate a traditional polymerase chain reaction (PCR) assay for the detection of pure and hybridized EWM DNA using both laboratory and field experiments. We performed a pilot experiment in outdoor tanks to determine the basic functionality and sensitivity of the assay. Following this initial test, we collected field samples from Michigan and Montana lakes with and without known EWM populations. Taken together, our findings suggest that eDNA techniques have potential to be a useful strategy for the early detection of EWM.

Journal of Aquatic Plant Management

Hydrology and simulation of ground-water flow in Cedar Valley, Iron County, Utah

Cedar Valley, located in the eastern part of Iron County in southwestern Utah, is experiencing rapid population growth. Cedar Valley traditionally has supported agriculture, but the growing population needs a larger share of the available water resources. Water withdrawn from the unconsolidated basin fill is the primary source for public supply and is a major source of water for irrigation. Water managers are concerned about increasing demands on the water supply and need hydrologic information to manage this limited water resource and minimize flow of water unsuitable for domestic use toward present and future public-supply sources. Surface water in the study area is derived primarily from snowmelt at higher altitudes east of the study area or from occasional large thunderstorms during the summer. Coal Creek, a perennial stream with an average annual discharge of 24,200 acre-feet per year, is the largest stream in Cedar Valley. Typically, all of the water in Coal Creek is diverted for irrigation during the summer months. All surface water is consumed within the basin by irrigated crops, evapotranspiration, or recharge to the ground-water system. Ground water in Cedar Valley generally moves from primary recharge areas along the eastern margin of the basin where Coal Creek enters, to areas of discharge or subsurface outflow. Recharge to the unconsolidated basin-fill aquifer is by seepage of unconsumed irrigation water, streams, direct precipitation on the unconsolidated basin fill, and subsurface inflow from consolidated rock and Parowan Valley and is estimated to be about 42,000 acre-feet per year. Stable-isotope data indicate that recharge is primarily from winter precipitation. The chloride mass-balance method indicates that recharge may be less than 42,000 acre-feet per year, but is considered a rough approximation because of limited chloride concentration data for precipitation and Coal Creek. Continued declining water levels indicate that recharge is not sufficient to meet demand. Water levels in many areas are at or close to historic lows. In 2000, withdrawal from wells was estimated to be 36,000 acre-feet per year. About 4,000 acre-feet per year are estimated to discharge to evapotranspiration or as subsurface outflow. Prior to large-scale ground-water development, ground-water discharge by evapotranspiration and discharge to springs was much larger. Ground water along the eastern margin of the valley between Cedar City and Enoch is unsuitable for domestic use because of high dissolved-solids and nitrate concentrations. The predominant ions of Ca and SO4 in this area indicate dissolution of gypsum in the Markagunt Plateau to the east. Data collected during this study were compared to historic data; there is no evidence to indicate deterioration in ground-water quality. The spatial distribution of ground water with high nitrate concentration does not appear to be migrating beyond its previously known extent. No single source can be identified as the cause for elevated nitrate concentrations in ground water. Low nitrogen-15 values north of Cedar City indicate a natural geologic source. Higher nitrogen-15 values toward the center of the basin and associated hydrologic data indicate probable recharge from waste-water effluent. Excess dissolved nitrogen gas and low nitrate concentrations in shallow ground water indicate that denitrification is occurring in some areas. A computer ground-water flow model was developed to simulate flow in the unconsolidated basin fill. The method of determining recharge from irrigation was changed during the calibration process to incorporate more areal and temporal variability. In general, the model accurately simulates water levels and water-level fluctuations and can be considered an adequate tool to help determine the valley-wide effects on water levels of additional ground-water withdrawals and changes in water use. The model was used to simulated water-level changes caused by projecting current withdrawal rates, increased withdrawal rates, and a 10-year drought. Water levels declined 20 to 275 feet in the southern and central parts of the valley and less than 20 feet north of Enoch

Utah

Changes in agriculture and abundance of snow geese affect carrying capacity of sandhill cranes in Nebraska

The central Platte River valley (CPRV) in Nebraska, USA, is a key spring-staging area for approximately 80 of the midcontinent population of sandhill cranes (Grus canadensis; hereafter cranes). Evidence that staging cranes acquired less lipid reserves during the 1990s compared to the late 1970s and increases in use of the CPRV by snow geese (Chen caerulescens) prompted us to investigate availability of waste corn and quantify spatial and temporal patterns of crane and waterfowl use of the region. We developed a predictive model to assess impacts of changes in availability of corn and snow goose abundance under past, present, and potential future conditions. Over a hypothetical 60-day staging period, predicted energy demand of cranes and waterfowl increased 87 between the late 1970s and 19982007, primarily because peak abundances of snow geese increased by 650,000 and cranes by 110,000. Compared to spring 1979, corn available when cranes arrived was 20 less in 1998 and 68 less in 1999; consequently, the area of cornfields required to meet crane needs increased from 14,464 ha in 1979 to 32,751 ha in 1998 and 90,559 ha in 1999. Using a pooled estimate of 88 kg/ha from springs 19981999 and 20052007, the area of cornfields needed to supply food requirements of cranes and waterfowl increased to 65,587 ha and was greatest in the eastern region of the CPRV, where an estimated 54 of cranes, 47 of Canada geese (Branta canadensis), 45 of greater white-fronted geese (Anser albifrons), and 46 of snow geese occurred during ground surveys. We estimated that a future reduction of 25 in available corn or cornfields would increase daily foraging flight distances of cranes by 2738. Crane use and ability of cranes to store lipid reserves in the CPRV could be reduced substantially if flight distance required to locate adequate corn exceeded a physiological maximum distance cranes could fly in search of food. Options to increase carrying capacity for cranes include increasing accessibility of cornfields by restoring degraded river channels to disperse roosting cranes and increasing wetland availability in the Rainwater Basin to attract snow geese using the CPRV. ?? The Wildlife Society.

Journal of Wildlife Management

Simulation of nutrient and sediment concentrations and loads in the Delaware inland bays watershed: Extension of the hydrologic and water-quality model to ungaged segments

Rapid population increases, agriculture, and industrial practices have been identified as important sources of excessive nutrients and sediments in the Delaware Inland Bays watershed. The amount and effect of excessive nutrients and sediments in the Inland Bays watershed have been well documented by the Delaware Geological Survey, the Delaware Department of Natural Resources and Environmental Control, the U.S. Environmental Protection Agency’s National Estuary Program, the Delaware Center for Inland Bays, the University of Delaware, and other agencies. This documentation and data previously were used to develop a hydrologic and water-quality model of the Delaware Inland Bays watershed to simulate nutrients and sediment concentrations and loads, and to calibrate the model by comparing concentrations and streamflow data at six stations in the watershed over a limited period of time (October 1998 through April 2000). Although the model predictions of nutrient and sediment concentrations for the calibrated segments were fairly accurate, the predictions for the 28 ungaged segments located near tidal areas, where stream data were not available, were above the range of values measured in the area. The cooperative study established in 2000 by the Delaware Department of Natural Resources and Environmental Control, the Delaware Geological Survey, and the U.S. Geological Survey was extended to evaluate the model predictions in ungaged segments and to ensure that the model, developed as a planning and management tool, could accurately predict nutrient and sediment concentrations within the measured range of values in the area. The evaluation of the predictions was limited to the period of calibration (1999) of the 2003 model. To develop estimates on ungaged watersheds, parameter values from calibrated segments are transferred to the ungaged segments; however, accurate predictions are unlikely where parameter transference is subject to error. The unexpected nutrient and sediment concentrations simulated with the 2003 model were likely the result of inappropriate criteria for the transference of parameter values. From a model-simulation perspective, it is a common practice to transfer parameter values based on the similarity of soils or the similarity of land-use proportions between segments. For the Inland Bays model, the similarity of soils between segments was used as the basis to transfer parameter values. An alternative approach, which is documented in this report, is based on the similarity of the spatial distribution of the land use between segments and the similarity of land-use proportions, as these can be important factors for the transference of parameter values in lumped models. Previous work determined that the difference in the variation of runoff due to various spatial distributions of land use within a watershed can cause substantialloss of accuracy in the model predictions. The incorporation of the spatial distribution of land use to transfer parameter values from calibrated to uncalibrated segments provided more consistent and rational predictions of flow, especially during the summer, and consequently, predictions of lower nutrient concentrations during the same period. For the segments where the similarity of spatial distribution of land use was not clearly established with a calibrated segment, the similarity of the location of the most impervious areas was also used as a criterion for the transference of parameter values. The model predictions from the 28 ungaged segments were verified through comparison with measured in-stream concentrations from local and nearby streams provided by the Delaware Department of Natural Resources and Environmental Control. Model results indicated that the predicted edge-of-stream total suspended solids loads in the Inland Bays watershed were low in comparison to loads reported for the Eastern Shore of Maryland from the Chesapeake Bay watershed model. The flatness of the terrain and the low annual surface runoff are important factors in determining the amount of detached sediment from the land that is delivered to streams. The highest predicted total suspended solids loads were found in the southern part of the watershed, where the values are associated with high total streamflow and a high surface-runoff component, and related to soil and aquifer permeability and land use. Nutrient loads from model segments in the southern part of the Inland Bays watershed were also higher than those measured in the northern part of the basin, due to relatively high runoff and the substantial amount of available organic fertilizer (animal waste) that results in over-application of organic fertilizer to crops. Time series of simulated hourly concentrations indicated a seasonal pattern in the simulated base flow for total nitrogen, with the lowest values occurring during the summer and the highest values during the winter months. Total phosphorus and total-suspended-solids concentrations were less seasonal and were more storm-dependent; in general, base-flow concentrations of total phosphorus and total suspended solids were low. During storm events, the total nitrogen concentrations tended to be diluted and total phosphorus concentrations tended to rise sharply. Nitrogen was transported mainly in the aqueous phase and largely through ground water, whereas phosphorus was strongly associated with sediment, which washes off during rainfall events.

Delaware

Selenium in ecosystems within the mountaintop coal mining and valley-fill region of southern West Virginia-assessment and ecosystem-scale modeling

Coal and associated waste rock are among environmental selenium (Se) sources that have the potential to affect reproduction in fish and aquatic birds. Ecosystems of southern West Virginia that are affected by drainage from mountaintop coal mines and valleys filled with waste rock in the Coal, Gauley, and Lower Guyandotte watersheds were assessed during 2010 and 2011. Sampling data from earlier studies in these watersheds (for example, Upper Mud River Reservoir) and other mining-affected watersheds also are included to assess additional hydrologic settings and food webs for comparison. Basin schematics give a comprehensive view of sampled species and Se concentration data specific to location and date. Food-web diagrams document the progression of Se trophic transfer across suspended particulate material, invertebrates, and fish for each site to serve as the basis for developing an ecosystem-scale model to predict Se exposure within the hydrologic conditions and food webs of southern West Virginia. This approach integrates a site-specific predator’s dietary exposure pathway into modeling to ensure an adequate link to Se toxicity and, thus, to species vulnerability. Site-specific fish abundance and richness data in streams documented various species of chub, shiner, dace, darters, bass, minnow, sunfish, sucker, catfish, and central stoneroller (Campostoma anomalum), mottled sculpin (Cottus bairdii), and least brook lamprey (Lampetra aepyptera). However, Se assessment species for streams, and hence, model species for streams, were limited to creek chub (Semotilus atromaculatus) and central stoneroller. Both of these species of fish are generally considered to have a high tolerance for environmental stress based on traditional comparative fish community assessment, with creek chub being present at all sites. Aquatic insects (mayfly, caddisfly, stonefly, dobsonfly, chironomid) were the main invertebrates sampled in streams. Collection of suspended particulate material acted as an integrator of organic-rich, fine-grained biomass present in streams. The base-case food web modeled for streams was suspended particulate material to aquatic insect to creek chub, with comparative modeling of a direct particulate-to-stoneroller food web. Model species for a reservoir setting were based on an earlier study of bluegill sunfish (Lepomis macrochirus), green sunfish (Lepomis cyanellus), and largemouth bass (Micropterus salmoides). Several reservoir food webs were considered based on a variety of invertebrates (insect, snail, clam). For stream and reservoir settings, predicted Se concentrations in exposure scenarios showed a high degree of correlation (r 2 = 0.91 for invertebrates and 0.75 for fish) with field observations of Se concentrations when modeling was initiated from suspended-particulate-material Se concentrations and model transfer parameters defined previously in the literature were used. These strong correlations validate the derived site-specific model and establish sufficient confidence that the predictions from the developed model can be quantitatively applied to the ecosystems in southern West Virginia. An application of modeling used a metric describing the partitioning of Se between particulate material and dissolved phases (K d ) to allow determination of a dissolved Se concentration that would be necessary to attain a site-specific Se fish body burden. The operationally defined K d quantifies the complex process of transformation at the base of a food web on a site-specific basis. The magnitude of this metric is known to vary with such factors as Se speciation, particulate-material type, and hydrology. This application (1) ties dissolved Se concentrations to fish tissue concentrations; (2) allows consideration of different choices for intervening site-specific exposure steps that set Se bioaccumulation, partitioning, and bioavailability; and (3) generates implications for management decisions that define protection through different regulatory pathways and guidelines. The range of model outcomes accounts for critical sources of variability and establishes whether site and food-web characterization were adequate to represent the dynamics of the system with certainty. This is especially true in terms of particulate-material phases at the base of the food web and utilization of K d in different hydrologic settings. For streams, a range of field-derived K d ds were applied to food-web exposure scenarios within a framework of locational and hydrologic variables (area of stream basin; stream gradient and discharge) that may affect the magnitude of K d . Overlaying even a coarse temporal scale that acknowledges variability in stream dissolved Se and Se speciation, such as through seasonal derivation of K d , can substantially narrow model uncertainty. Modeling that constrains the place and time of greatest ecosystem Se sensitivity within a specified food web gives insight into Se risk and identifies controlling management alternatives within a watershed or stream basin. If there is a range of hydrologic settings, specificity is needed to establish a hierarchy of in-stream and off-stream habitats for a watershed approach that takes into account Se-enriched water moving through different K d and food web environments. If there is a range of predator vulnerabilities (measured as a combination of food-web Se biodynamics and response in Se toxicity tests) within the site-specific community of fish species to be protected, then choice of fish species is critical to protection because it determines the food web and, hence, the magnitude of biotransfer through which Se is modeled. Whether creek chub is representative of the vulnerability to Se of all fish species encountered within the study-site ecosystems will require additional species-specific data and analysis. A range of site-specific scenarios illustrated here set model outcomes, but the final quantitative evaluation of alternatives and their implications will be those generated through choices and guidance formulated by state and other agencies in their decisionmaking processes. Proposed additions and refinements to the ecosystem-scale site-specific approach developed here include consideration of: measurement of temporally matched pairs of dissolved and suspended-particulate-material Se concentrations across a broader range of stream sites to expand the stream K d database and to test the representativeness of a suspended-particulate-material sample within a stream; characterization of different phases of particulate material across seasons to better define the base of the food web and connect to invertebrate feeding; refinement of model assumptions concerning dietary preferences and composition for fish to develop additional trophic transfer factors (TTFs) (for example, calculation of TTFinvertebrate composite for mixed diets); expansion of modeling of fish species and their food webs to include Se-vulnerable species; temporal characterization of a predator’s life cycle and habitat use as additional model layers to integrate with Se biodynamics in streams; investigation of the effect of stream gradient on K d based on a finer scale than presented here in terms of such variables as residence time, watershed dilution, and physical habitat attributes (for example, amount of ponding versus run or riffle within a stream); and linkage to discharge through use of stream gaging to record variability and enable model organization within water-year types and discharge seasons. Investigating the presence and variability of prey and predator species in demographically open systems such as streams also is key to model outcomes given the overall environmental stressors (for example, general landscape change, food-web disruption, recolonization potential) imposed on the composition of biological communities in coal mining and valley-fill affected watersheds

West Virginia