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At least 433 records · Page 24Linked to original sources

Efficacy of oblique bubble screen deterrents on redirecting live eggs and larvae of grass carp (Ctenopharyngodon idella) at different developmental stages

Invasive carp have severely damaged aquatic ecosystems in the USA, particularly in the Mississippi River Basin. Behavioral deterrents have been developed in the last few decades to control population expansion into new ecosystems. However, none of these deterrents are capable of controlling early-life stage carp, which have limited or no mobility during their drifting stage in rivers. Capturing eggs and larvae in large numbers warrants new methods due to their distinct physical and biological properties as well as their lack of behavioral response. We tested a novel method to redirect downstream drifting eggs and larvae for their efficient removal in streams using an oblique bubble screen (OBS) deterrent. We investigated the effects of mean water velocity and airflow rate on redirection of eggs, pre-gas bladder inflation (GBI) larvae, near-GBI larvae, and dead larvae. Although similar OBS configurations had shown high efficacy redirecting plastic spheres (egg surrogates) in previous studies, they underperform redirecting live eggs and larvae. However, distinct patterns were identified for eggs, pre-GBI, near-GBI, and dead larvae. A detailed hydrodynamic analysis showed that eggs closely follow the larger scales of motions created by the OBS, and that larvae can actively respond to turbulence cues. This study yielded new insights into the movement of early-life stage grass carp in a turbulent flow with strong recirculation, and provided important data to improve the design of bubble screen dispersal barriers for invasive carp management and population control.

Water Resources Research

Changes in habitat use and distribution of mouflon in the Kahuku Unit of Hawai‘i Volcanoes National Park

European mouflon sheep ( Ovis gmelini musimon ) were introduced to Kahuku Ranch on Hawai‘i Island in 1968 and 1974 for trophy hunting and have been detrimental to the native ecosystem by trampling, bark stripping, and browsing vegetation. In 2003, Hawai‘i Volcanoes National Park acquired Kahuku Ranch and managers began removing mouflon. The objective of this project was to determine whether hunting has changed the distribution of mouflon in Kahuku, to better understand mouflon behaviour and to expedite eradication efforts. Locations from hunting and GPS telemetry data during 2007–14 were used to determine the effect of hunting on mouflon distribution by examining distance to roads and habitat use. Mouflon seemed to avoid roads after hunting pressure increased and their distribution within vegetation types changed over time. Mouflon without hunting pressure were detected in native shrub habitat in 68% of all observations. Hunted mouflon were encountered less in native shrub habitat and more in other habitats including open forest, closed forest, and areas with no vegetation. These changes suggest that hunting has influenced the distribution of mouflon over time away from native shrub and into other vegetation types where they may be more difficult to control.

Hawai‘i

Long-term experimental manipulation of winter snow regime and summer temperature in arctic and alpine tundra

Three 60 m long, 2·8 m high snowfences have been erected to study long-term effects of changing winter snow conditions on arctic and alpine tundra. This paper describes the experimental design and short-term effects. Open-top fiberglass warming chambers are placed along the experimental snow gradients and in controls areas outside the fences; each warming plot is paired with an unwarmed plot. The purpose of the experiment is to examine short- and long-term changes to the integrated physical-biological systems under simultaneous changes of winter snow regime and summer temperature, as part of the Long-Term Ecological Research network and the International Tundra Experiment. The sites were at Niwot Ridge, Colorado, a temperate high altitude site in the Colorado Rockies, and Toolik Lake, Alaska, a high-latitude site. Initial results indicate that although experimental designs are essentially identical at the arctic and alpine sites, experimental effects are different. The drift at Niwot Ridge lasts much longer than do the Toolik Lake drifts, so that the Niwot Ridge fence affects both summer and winter conditions, whereas the Toolik Lake fence affects primarily winter conditions. The temperature experiment also differs in effect between the sites. Although the average temperature increase at the two sites is similar (daily increase 1·5 °C at Toolik and 1·9 °C at Niwot Ridge), at Toolik Lake there is only minor diurnal variation, whereas at Niwot Ridge the daytime increases are extreme on sunny days (as much as 7–10 °C), and minimum nighttime temperatures in the chambers are often slightly cooler than ambient (by about 1 °C). The experimental drifts resulted in wintertime increases in temperature and CO 2 flux. Temperatures under the deep drifts were much more consistent and warmer than in control areas, and at Niwot Ridge remained very close to 0 °C all winter. These increased temperatures were likely responsible for observed increases in system carbon loss. Initial changes to the aboveground biotic system included an increase in growth in response to both snow and warming, despite a reduced growing season. This is expected to be a transient response that will eventually be replaced by reduced growth. At least one species, Kobresia myosuroides , had almost completely died at Niwot Ridge three years after fence construction, whereas other species were increasing. We expect in both the short- and long-term to see the strongest effects of snow at the Niwot Ridge site, and stronger effects of temperature at Toolik Lake.

Hydrological Processes

Monitoring the effect of deep drawdowns of a flood control reservoir on sediment transport and dissolved oxygen, Fall Creek Lake, Oregon

Annual reservoir drawdowns at Fall Creek Lake, Oregon, have occurred for eight consecutive years from December 2012 to November 2019. The annual drawdowns are the result of the 2008 Biological Opinion of the US Army Corps of Engineers (USACE) Willamette Valley Project operations, which directed the USACE to carry out interim operational measures that would provide volitional downstream passage for endangered species act (ESA)-listed Chinook salmon. At Fall Creek Lake, the USACE modifies its operations by lowering the reservoir elevation to 690-ft, approximately 40 feet below the normal winter low-pool elevation. This action results in a runof-river scenario through the dam allowing juvenile Chinook salmon to safely pass through the regulating outlets. Monitoring of juvenile Chinook salmon in screw traps at the outlet of the dam has shown variable timing in out-migration associated with reservoir elevation, and that most of the juvenile fish exited the reservoir when the pool elevation passed 700-ft (Taylor and others, 2015). The annual drawdown has therefore been effective in providing safe downstream fish passage and has also had the collateral effect of transporting large quantities of suspended sediment to the downstream reaches of Fall Creek and the Middle Fork Willamette River. The US Geological Survey (USGS) has calculated time-series of suspended sediment concentrations (SSC) and suspended sediment loads (SSL) before, during, and after the drawdowns for six of the last nine drawdown years (water years [WY] 2013-2018), which have lasted between 5-14 days. The transport and deposition of sediment from the drawdowns has affected side-channel habitat below the dam by depositing large quantities of sand-size material resulting in streambed aggradation in several locations. The results from the USGS monitoring effort have provided important information to USACE on how the modification of their operations has affected sediment transport in the river reaches below the dam.

Oregon

Interdisciplinary science approach for harmful algal blooms (HABs) and algal toxins—A strategic science vision for the U.S. Geological Survey

Executive Summary Algal blooms in water, soils, dusts, and the environment have captured national attention because of concerns associated with exposure to algal toxins for humans and animals. Algal blooms naturally occur in all surface-water types and are important primary producers for aquatic ecosystems. However, excessive algae growth can be associated with many harmful effects ranging from aesthetic to toxicity concerns, so this excessive growth is commonly called a harmful algal bloom (HAB). Ecological imbalances that can lead to excessive algal growth, such as increased nutrient availability to waterbodies from natural and anthropogenic sources, are well documented in scientific literature. On the other hand, fundamental scientific understandings of environmental causes and controls leading to algal toxin production, environmental exposures, and adverse health outcomes for humans and animals could benefit from more attention by U.S. Geological Survey (USGS) scientists. Understanding when, why, and how the toxin is produced by individual algal cells or communities and why the toxin is released to the surrounding waterbody requires fundamental research to determine a toxin’s role, whether it provides competitive advantage or if other potential reasons exist for toxin production and release, such as secretions from otherwise benign biological processes. This research will require groundbreaking scientific discovery about underlying biologic and abiotic (non-living) processes commonly complicated by local variation in land use, microbial species composition, and ecosystem structure of the surrounding watershed. Although underlying processes by which HABs form may be similar from one waterbody to another, individual waterbodies may be controlled by local factors for HAB development and toxin production that are unique to the watershed. Consequently, many fundamental science gaps exist that prevent informed mitigation and prevention of toxic HAB events. There are also gaps in understanding local conditions that control algal growth unique to specific watersheds. Addressing these science gaps is needed to inform evidence-based decisions that protect human and animal health and that reduce recreational and socioeconomic losses.

Circular

TiO 2 as a photocatalyst for control of the aquatic invasive alga, Cladophora, under natural and artificial light

Cladophora, a nuisance and invasive, filamentous algae (Chlorophyta), massively accumulates along the shores of the lower Great Lakes each summer causing great economic damage and compromising recreational opportunity and perhaps public health. In vitro experiments showed that Cladophora samples were physically and biologically degraded when subjected to TiO 2 -mediated photocatalysis. For the most successful photocatalytic process, TiO 2 was immobilized on a glass surface and used in combination with either sunlight or artificial UV light. The loss of vital algal pigments was monitored using UV–vis spectrophotometry, and cell structural changes were determined by microscopic observation. Cladophora, in the presence of TiO 2 -covered glass beads, experienced a loss of chloroplast pigments after 2 h of UV lamp light irradiation. In a separate experiment, sunlight exposure over 4 days (∼24 h) resulted in the complete oxidative degradation of the green chloroplast pigments, verified by the UV spectra of the algal extracts. These results suggest that TiO 2 , mobilized on sunlit silicates may be useful in controlling growth and survival of this alga in the Great Lakes, thus mitigating many of the economic, aesthetic ecological impacts of this invasive alga.

Journal of Photochemistry and Photobiology A: Chem

Lead exposure of a fossorial rodent varies with the use of ammunition across the landscape

Exposure to heavy metals has been documented in a wide range of wildlife species, but infrequently in ground squirrels. This is despite their tendency to be targets of recreational shooters and the accumulation of lead ammunition in the soil environments they inhabit. We analyzed lead and copper concentrations in liver (n Pb = 116, n Cu = 101) and femur (n Pb = 116, n Cu = 116) of Piute ground squirrels ( Urocitellus mollis ) and in soil ( n = 75) on public lands in southwestern Idaho to understand how lead exposure may vary across a gradient of intensities and histories of shooting activity. The liver and femur of squirrels from areas used for recreational shooting for greater than 30 years had elevated lead concentrations relative to areas where shooting was rare or did not occur (our negative control), but as expected, lower than areas used for military target training for greater than 70 years (our positive control). Lead concentration in soils were higher in areas used for military target training than in those used for recreational shooting. There were no differences in copper concentrations in biological or soil samples among sites. These data suggest that ground squirrels can be influenced by the history of lead use in their local environment, and they illustrate another pathway by which human activity can influence toxicant exposure to wildlife.

Science ot the Total Environment

Nonequilibrium clumped isotope signals in microbial methane

Methane is a key component in the global carbon cycle with a wide range of anthropogenic and natural sources. Although isotopic compositions of methane have traditionally aided source identification, the abundance of its multiply-substituted “clumped” isotopologues, e.g., 13 CH 3 D, has recently emerged as a proxy for determining methane-formation temperatures; however, the impact of biological processes on methane’s clumped isotopologue signature is poorly constrained. We show that methanogenesis proceeding at relatively high rates in cattle, surface environments, and laboratory cultures exerts kinetic control on 13 CH 3 D abundances and results in anomalously elevated formation temperature estimates. We demonstrate quantitatively that H 2 availability accounts for this effect. Clumped methane thermometry can therefore provide constraints on the generation of methane in diverse settings, including continental serpentinization sites and ancient, deep groundwaters.

Science

Interaction of a legacy groundwater contaminant plume with the Little Wind River from 2015 through 2017, Riverton Processing site, Wyoming

The Riverton Processing site was a uranium mill 4 kilometers southwest of Riverton, Wyoming, that prepared uranium ore for nuclear reactors and weapons from 1958 to 1963. The U.S. Department of Energy completed surface remediation of the uranium tailings in 1989; however, groundwater below and downgradient from the tailings site and nearby Little Wind River was not remediated. Beginning in 2010, a series of floods along the Little Wind River began to mobilize contaminants in the unsaturated zone, resulting in substantial increases of uranium and other contaminants of concern in monitoring wells completed inside the contaminant plume. In 2011, the U.S. Department of Energy started a series of university and Government agency retrospective and field investigations to understand the processes controlling contaminant increases in the groundwater plume. The goals of the field investigations described in this report were to (1) identify and quantify the contaminant flux and potential associated biological effects from groundwater associated with the legacy plume as it enters a perennial stream reach, and (2) assess chemical exposure and potential effects to biological receptors from the interaction of the contaminant plume and the river. Field investigations along the Little Wind River were completed by the U.S. Geological Survey during 2015–17 in cooperation with the U.S. Department of Energy Office of Legacy Management to characterize: (1) seepage areas and seepage rates; (2) pore-water and bed sediment chemistry and hyporheic exchange and reactive loss; and (3) exposure pathways and biological receptors. All data collected during the study are contained in two U.S. Geological Survey data releases, available at https://doi.org/10.5066/F7BR8QX4 and https://doi.org/10.5066/P9J9VJBR . A variety of tools and methods were used during the field characterizations. Streambed temperature mapping, electrical resistivity tomography, electromagnetic induction, fiber-optic distributed temperature sensing, tube seepage meters, vertical thermal sensor arrays, and an environmental tracer (radon) were used to identify areas of groundwater seepage and associated seepage rates along specific sections of the study reach of the river. Drive points, minipiezometers, diffusive equilibrium in thin-film/diffusive gradients in thin-film probes, bed-sediment samples, and equal discharge increment sampling methods were used to characterize pore-water chemistry, estimate hyporheic exchange and reactive loss of selected chemical constituents, and quantify contaminant loadings entering the study reach. Sampling and analysis of surface sediments, filamentous algae, periphytic algae, and macroinvertebrates were used to characterize biological exposure pathways, metal uptake, and receptors. Areas of focused groundwater discharge identified by the fiber-optic distributed temperature sensing surveys corresponded closely with areas of elevated electrical conductivity identified by the electromagnetic induction survey results in the top 5 meters of sediment. During three monitoring periods in 2016, the mean vertical seepage rate measured with tube seepage meters was 0.45 meter per day, ranging from −0.02 to 1.55 meters per day. Five of the 11 locations where vertical thermal profile data were collected along the study reach during August 2017 indicated mean upwelling values ranging from 0.11 to 0.23 meter per day. Radon data collected from the Little Wind River during June, July, and August 2016 indicated a consistent inflow of groundwater to the central part of the study reach, in the area congruous with the center of the previously mapped groundwater plume discharge zone. During August 2017, the greatest attenuation of uranium from reactive loss in pore-water samples was observed at three locations along the study reach, at depths between 6 and 15 centimeters, and similar trends in molybdenum attenuation were also observed. Bed-sediment concentration profiles collected during 2017 also indicated attenuation of uranium and molybdenum from groundwater during hyporheic mixing of surface water with the legacy plume during groundwater upwelling into the river. Streamflow measurements combined with equal discharge increment water sampling along the study reach indicated an increase in dissolved uranium concentrations in the downstream direction during 2016 and 2017. Net uranium load entering the Little Wind River study reach was about 290 and 435 grams per day during 2016 and 2017, respectively. Biological samples indicated that low levels of uranium and molybdenum exposure were confined to the benthos in the Little Wind River within and immediately downstream from the perimeter of the groundwater plume. Concentrations of molybdenum and uranium in filamentous algae were consistently low at all sites in the study reach with no indication of increased exposure of dissolved bioavailable molybdenum or uranium at sites next to or downstream from the groundwater plume. Comparison of the August 2017 results from electromagnetic induction, tube seepage meters, vertical thermal profiling, and pore-water chemistry surveys were in general agreement in identifying areas with upwelling groundwater conditions along the study reach. However, the electroconductivity values measured with electromagnetic induction in the top 100 centimeters of sediment did not agree with sodium concentrations measured in pore-water samples collected at similar streambed depths. Differences and similarities between multiple methods can result in additional insights into hydrologic and biogeochemical processes that may be occurring along a reach of a river system interacting with shallow groundwater inputs. It may be advantageous to apply a variety of geophysical, geochemical, hydrologic, and biological tools at other Uranium Mill Tailings Remedial Action ( https://www.energy.gov/sites/prod/files/2014/10/f19/UMTRCA.pdf ) sites during the investigation of legacy contaminant plume interactions with surface-water systems.

Wyoming

Pinyon-juniper woodlands

Pinyon-juniper woodlands are one of the largest ecosystems in the Southwest and in the Middle Rio Grande Basin (Fig. 1). The woodlands have been important to the region's inhabitants since prehistoric times for a variety of natural resources and amenities. The ecosystems have not been static; their distributions, stand characteristics, and site conditions have been altered by changes in climatic patterns and human use and, often, abuse. Management of these lands since European settlement has varied from light exploitation and benign neglect, to attempts to remove the trees in favor of forage for livestock, and then to a realization that these lands contain useful resources and should be managed accordingly. Land management agencies are committed to ecosystem management. While there are several definitions of ecosystem management, the goal is to use ecological approaches to create and maintain diverse, productive, and healthy ecosystems (Kaufmann et al. 1994). Ecosystem management recognizes that people are an integral part of the system and that their needs must be considered. Ecological approaches are central to the concept, but our understanding of basic woodland ecology is incomplete, and there are different opinions and interpretations of existing information (Gottfried and Severson 1993). There are many questions concerning proper ecosystem management of the pinyon-juniper woodlands and how managers can achieve these goals (Gottfried and Severson 1993). While the broad concept of ecosystem management generally is accepted, the USDA Forest Service, other public land management agencies, American Indian tribes, and private landowners may have differing definitions of what constitutes desired conditions. Key questions about the pinyon-juniper ecosystems remain unanswered. Some concern the basic dynamics of biological and physical components of the pinyon-juniper ecosystems. Others concern the distribution of woodlands prior to European settlement and changes since the introduction of livestock and fire control. This relates to whether tree densities have been increasing or whether trees are invading grasslands and, to a lesser extent, drier ponderosa pine (Pinus ponderosa) forests. In areas where woodlands were heavily used by American Indians for fuelwood prior to European contact, the advance of pinyon and juniper could represent the slow recovery from intensive use (Samuels and Betancourt 1982). There are numerous questions regarding declines in watershed condition related to changes in pinyon-juniper tree stand densities and to the density and composition of understory vegetation. There are different opinions about proper management of woodland ecosystems. Should these lands be managed for a single resource, such as forage for livestock production, or managed for sustained production of multiple resource products and amenities? Depending on site and stand conditions, the woodlands can produce variable quantities of fuelwood, pinyon nuts, wildlife habitat, forage for livestock, and cover for watershed protection. Management must also consider increasing recreational demands, threatened and endangered species, and protection of archeological sites. Many pinyon-juniper woodland watersheds in New Mexico have unsatisfactory soil and watershed conditions (USDA Forest Service 1993); managers must develop restoration procedures that recognize the value of woodland ecosystems. The concerns, questions, and conflicts surrounding management of pinyon-juniper lands, as well as the ecological foundations of ecosystem management, require that all interested parties reevaluate attitudes toward the woodlands. Ecosystem management goals and concepts recognize diversity. Pinyonjuniper woodlands are diverse, and stand characteristics and site productivities vary. Management objectives and prescriptions must evaluate the potential of each site, and decisions must be based on sound scientific information. This information is often unavailable. Therefore, this paper describes what we do know about the characteristics, distribution, and ecology of pinyon-juniper woodlands, including the effects of natural and human factors, within the southwestern United States and particularly the Middle Rio Grande Basin. It also reviews some past and present management options in this widespread and important vegetation type. The review draws on research and management information from the Rio Grande Basin and from similar areas in the Southwest and adjacent regions. It does not attempt to review all of the relevant literature; additional sources can be found within the articles cited in the References.

General Technical Report

Visualization of Flow Alternatives, Lower Missouri River

Background The U.S. Army Corps of Engineers (COE) 'Missouri River Master Water Control Manual' (Master Manual) review has resulted in consideration of many flow alternatives for managing the water in the river (COE, 2001; 1998a). The purpose of this report is to present flow-management alternative model results in a way that can be easily visualized and understood. This report was updated in October 2001 to focus on the specific flow-management alternatives presented by the COE in the 'Master Manual Revised Draft Environmental Impact Statement' (RDEIS; COE, 2001). The original version (February 2000) is available by clicking here. The COE, U.S. Fish and Wildlife Service (FWS), Missouri River states, and Missouri River basin tribes have been participating in discussions concerning water management of the Missouri River mainstem reservoir system (MRMRS), the Missouri River Bank Stabilization and Navigation Project, and the Kansas River reservoir system since 1986. These discussions include general input to the revision of the Master Manual as well as formal consultation under Section 7 of the Endangered Species Act. In 2000, the FWS issued a Biological Opinion that prescribed changes to reservoir management on the Missouri River that were believed to be necessary to preclude jeopardy to three endangered species, the pallid sturgeon, piping plover, and interior least tern (USFWS, 2000). The combined Missouri River system is large and complex, including many reservoirs, control structures, and free-flowing reaches extending over a broad region. The ability to assess future impacts of altered management scenarios necessarily involves complex, computational models that attempt to integrate physical, chemical, biological, and economic effects. Graphical visualization of the model output is intended to improve understanding of the differences among flow-management alternatives.

Colorado, Iowa, Kansas, Missouri, Montana, Nebrask

Can we manage for biological diversity in the absence of science?

Conservation of biological diversity is dependent on sound scientific information about underlying ecological processes. Current knowledge of the composition, distribution, abundance and life cycles of most species of plants and animals is incomplete, insufficient, unreliable, or nonexistent. Contemporary managers are also confronted with additional levels of complexity related to varying degrees of knowledge and understanding about interactions of species and ecosystems. Consequently, traditional species-oriented management schemes may have unintended consequences and ecosystem-oriented management initiatives may fail in the face of inadequate or fragmentary information on the structure, function, and dynamics of biotic communities and ecological systems. Nevertheless, resource managers must make decisions and manage based on the best biological information currently available. Adaptive resource management may represent a management paradigm that allows managers to learn something about the species or systems that they are managing while they are managing, but potential pitfalls lurk for such approaches. In addition to lack of control over the primary physical, chemical, and ecological processes, managers also lack control over social, economic, and political parameters affecting resource management options. Moreover, appropriate goals may be difficult to identify and criteria for determining success may be elusive. Some management responsibilities do not lend themselves to adaptive strategies. Finally, the lessons learned from adaptive management are usually obtained from a highly situational context that may limit applicability in a wider range of situations or undermine confidence that problems and solutions were properly diagnosed and addressed. Several scenarios are critically examined where adaptive management approaches may be inappropriate or ineffective and where management for biological diversity may be infeasible or inefficient without a sound scientific basis. Whereas some level of management must exist to meet agency responsibilities, more research is needed to conserve biological diversity.

Wildlife Society Annual Conference

Potential effects of climate change on surface-water quality in North America

Data from long-term ecosystem monitoring and research stations in North America and results of simulations made with interpretive models indicate that changes in climate (precipitation and temperature) can have a significant effect on the quality of surface waters. Changes in water quality during storms, snowmelt, and periods of elevated air temperature or drought can cause conditions that exceed thresholds of ecosystem tolerance and, thus, lead to water-quality degradation. If warming and changes in available moisture occur, water-quality changes will likely first occur during episodes of climate-induced stress, and in ecosystems where the factors controlling water quality are sensitive to climate variability. Continued climate stress would increase the frequency with which ecosystem thresholds are exceeded and thus lead to chronic water-quality changes. Management strategies in a warmer climate will therefore be needed that are based on local ecological thresholds rather than annual median condition. Changes in land use alter biological, physical, and chemical processes in watersheds and thus significantly alter the quality of adjacent surface waters; these direct human-caused changes complicate the interpretation of water-quality changes resulting from changes in climate, and can be both mitigated and exacerbated by climate change. A rigorous strategy for integrated, long-term monitoring of the ecological and human factors that control water quality is necessary to differentiate between actual and perceived climate effects, and to track the effectiveness of our environmental policies.

Journal of the American Water Resources Associatio

Diversity of fungal endophytes in non-native Phragmites australis in the Great Lakes

Plant–microbial interactions may play a key role in plant invasions. One common microbial interaction takes place between plants and fungal endophytes when fungi asymptomatically colonize host plant tissues. The objectives of this study were to isolate and sequence fungal endophytes colonizing non-native Phragmites australis in the Great Lakes region to evaluate variation in endophyte community composition among three host tissue types and three geographical regions. We collected entire ramets from multiple clones and populations, surface sterilized plant tissues, and plated replicate tissue samples from leaves, stems, and rhizomes on corn meal agar plates to culture and isolate fungal endophytes. Isolates were then subjected to Sanger sequencing of the ITS region of the nuclear ribosomal DNA. Sequences were compared to fungal databases to define operational taxonomic units (OTUs) that were analyzed statistically for community composition. In total, we obtained 173 endophyte isolates corresponding to 55 OTUs, 39 of which were isolated only a single time. The most common OTU corresponded most closely to Sarocladium strictum and comprised 25 % of all fungal isolates. More OTUs were found in stem tissues, but endophyte diversity was greatest in rhizome tissues. PERMANOVA analyses indicated significant differences in endophyte communities among tissue types, geographical regions, and the interaction between those factors, but no differences among individual ramets were detected. The functional role of the isolated endophytes is not yet known, but one genus isolated here ( Stagonospora ) has been reported to enhance Phragmites growth. Understanding the diversity and functions of Phragmites endophytes may provide targets for control measures based on disrupting host plant/endophyte interactions.

Great Lakes region

Managing an invasive corallimorph at Palmyra Atoll National Wildlife Refuge, Line Islands, Central Pacific

In 2007, a phase shift from corals to corallimorpharians (CM) centered around a shipwreck was documented at Palmyra Atoll, Line Islands. Subsequent surveys revealed CM to be overgrowing the reef benthos, including corals and coralline algae, potentially placing coral ecosystems in the atoll at risk. This prompted the U.S. Fish and Wildlife Service, the lead management agency of the atoll, to remove the shipwreck. Subsequent surveys showed reductions in CM around the ship impact site. We explain patterns of spread of the CM in terms of both life history and local currents and show with a pilot study that pulverized bleach may be an effective tool to eradicate CM on a local scale. If applied strategically, particularly in heavily infested (> 66% cover) areas, active intervention such as this could be an effective management tool to reduce CM impact on localized areas and decrease colonization rate of remaining reefs. This is the first documentation of the response of an invasive cnidarian to shipwreck removal. While this was a singular event in Palmyra, the spatial and temporal patterns of this invasion and the eradications lessons described herein, are useful for anticipating and controlling similar situations elsewhere.

Palmyra Atoll National Wildlife Refuge

The supply and demand for pollution control: Evidence from wastewater treatment

This paper analyzes the determination of pollution control from wastewater treatment plants as an economic decision facing local or regional regulators. Pollution control is measured by plant design effluent concentration levels and is fully endogenous in a supply- and-demand model of treatment choice. On the supply side, plant costs are a function of the design treatment level of the plant, and on the demand side, treatment level is a function of both the costs of control and the regional or regulatory preferences for control. We find evidence that the economic model of effluent choice by local regulators has a good deal of explanatory power. We find evidence that wastewater treatment plant removal of biological oxygen demand (BOD) is sensitive to many local factors including the size of the treatment plant, the flow rate of the receiving water, the population density of the surrounding area, regional growth, state sensitivity to environmental issues, state income, and the extent to which the damages from pollution fall on other states. We find strong evidence that regulators are sensitive to capital costs in determining the design level of BOD effluent reduction at a plant. Thus, proposed reductions in federal subsidies for wastewater treatment plant construction are likely to have significant adverse effects on water quality.

Journal of Environmental Economics and Management

Effects of carbon dioxide variations in the unsaturated zone on water chemistry in a glacial-outwash aquifer

The research site at Otis Air Base, Cape Cod, Massachusetts, has been developed for hydrogeological and geochemical studies of sewage-effluent contaminated groundwater since 1982. Research of hydrologic properties, transport, and chemical and biological processes is ongoing, but the origin of background water chemistry has not been determined. The principal geochemical process giving rise to the observed background water chemistry is CO2-controlled hydrolysis of Na feldspar. Geochemical modeling demonstrated that CO2 sources could vary over the project area. Analyses of unsaturated zone gases showed variations in CO2 which were dependent on land use and vegetative cover in the area of groundwater recharge. Measurements of CO2 in unsaturated-zone gases showed that concentrations of total inorganic C in recharge water should range from about 0.035 to 1.0 mmoles/L in the vicinity of Otis Air Base. Flux of CO2 from the unsaturated zone varied for a principal land uses, ranging from 86 gC/m2/yr for low vegetated areas to 1630 gC/m2/yr for a golf course. Carbon dioxide flux from woodlands was 220 gC/m2/yr, lower than reported fluxes of 500 to 600 gC/m2/yr for woodlands in a similar climate. Carbon dioxide flux from grassy areas was 540 gC/m2/yr, higher than reported fluxes of 230 to 490 gC/m2/yr for grasslands in a similar climate.

Applied Geochemistry

Germination and establishment of the native plains cottonwood ( Populus deltoides Marshall subsp. monilifera ) and the exotic Russian-olive ( Elaeagnus angustifolia L.)

Russian-olive ( Elaeagnus angustifolia ) is a small Eurasian tree that has escaped from cultivation and become naturalized, primarily along watercourses throughout the western United States. We examined germination and establishment of Russian-olive and plains cottonwood ( Populus deltoides ), the principal native riparian tree of the Great Plains, under a range of experimental moisture and light conditions. The fewest seedings established under the driest conditions; seedling biomass was predictably lower in the shade; root-to-shoot ratios were higher for cottonwood, higher in the sun, and higher under drier conditions. Several interactions were also significant. The timing of germination and mortality varied between plains cottonwood and Russian-olive: cottonwood germinated in mid-June in all treatments in a single pulse with subsequent mortality; the timing and amount of Russian-olive germination differed substantially across treatments with little net mortality. Differences in life-history traits of these species, including seed size, viability, and dispersal, help explain treatment differences. Russian-olive will likely remain an important component of riparian communities along both unregulated and regulated western rivers because it succeeds under conditions optimal for cottonwood establishment and under many conditions unfavorable for cottonwood. Furthermore, many western states still encourage planting of Russian-olive, and control techniques tend to be labor-intensive and expensive .

Conservation Biology