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373 records · Page 10Linked to original sources
USGS2024

Ambient flow and transport in long-screened, sand-packed wells: Insights into cross contamination and wellbore flow

The presence of long-screened wells with a surrounding sand pack can have a major effect on the redistribution of contaminants in groundwater, particularly when the wells are set in low-hydraulic conductivity aquifers. Such redistribution, or cross contamination, can occur through vertical flow and advective transport or by in-well mixing via multiple non-advective transport processes. A multi-method approach, including the use of single borehole dilution tracer (SBDT) logging, was undertaken to estimate vertical transport of trichloroethylene (TCE) in 8 discontinued remedial extraction wells, all constructed with long screens (100 ft, or 30.6 m long) and surrounding sand packs, at Site 25, Edwards Air Force Base, California. The site is within an enclosed drainage basin that is underlain primarily by quartz-monzonite-granitic rocks in various states of weathering. Prior to this study, little information was available on the depths of fracture zones intersecting the wellbores. Results indicate that because of in-well mixing processes, a potential redistribution of TCE of up to 9 g/d per well occurs as a consequence of leaving the wells inactive (unpumped) and unsealed, as measured by SBDT logging. Simulations of flow made with a generic model of the site show that if the wells were to be sealed with well liners, with the intent of reducing vertical TCE transport but the sand pack left intact, TCE transport decreases by 53% overall compared to leaving the wells unlined.

Environmental Earth Sciences↗
USGS2026

An improved empirical model for predicting postfire debris-flow volume in the western United States

Reliable estimates of debris-flow volume can be used to help predict the magnitude of debris-flow hazards following wildfire in the western United States. In this study, we compiled and used a database of 227 postfire debris-flow volumes that were collected across the western United States to develop a multiple linear regression model for predicting postfire debris-flow volume. We explored 36 predictor variables related to rainfall, terrain, and fire characteristics, and selected the model with the combination of variables that yielded the most accurate predictions of debris-flow volume. We evaluated model performance against the entire volume database, as well as against four subsets of volume data from southern California, the Intermountain West, the Southwest, and regions with limited volume data, such as northern California and Washington. We also compared model performance against 3 existing postfire debris-flow volume models that were developed for use in southern California, the Intermountain West, and the Southwest. We demonstrate that the new volume model performs as well as the regional models in the regions for which they were developed and outperforms existing models when applied to volumes from data-limited regions in the western United States. These results indicate that the debris-flow volume model introduced in this study can be used to improve postfire hazard assessments across the western United States, especially outside of southern California.

Arizona, California, Colorado, New Mexico, Utah, W↗
USGS2025

An exploration of the relative influence of physical models for Omori’s law

Omori’s law states that the rate of aftershocks decays as a function of inverse time. There are multiple physical explanations that we reduce into a nonlinear mixed effects relation of three terms: (1) a Rate/State expression that can account for static/dynamic and viscoelastic triggering caused directly by the mainshock, (2) a fluid diffusion triggering term, and (3) a randomized secondary triggering (cascade) term. We fit free physical-model parameters to an observed aftershock sequence through two nonlinear regression methods to find the relative contributions of physics-based models in an observed aftershock sequence. Results from both methods show that Rate/State models overpredict aftershock rates by ∼0–30%. Secondary aftershocks cause a net negative contribution (seismicity rate reduction that corrects overprediction by other terms) ranging between ∼0 and 30%. All regression solutions yield negative secondary triggering contributions without being guided to do so. A physical explanation for this is that aftershock occurrence relieves stress from the crust, ultimately causing the sequence to extinguish itself. Fluid diffusion triggering contributions range from ∼0 to 20%. Diffusion processes are observed to be shorter in time than the full duration of an aftershock sequence and they are also spatially limited, diminishing their influence. Our results apply to an aftershock decay curve from the 2016 Central Apennines earthquake sequence, meaning that our specific results may not be general. Our primary conclusion is that any one physical model cannot alone fit the observed sequence as well as the combination of three we investigated.

Frontiers in Earth Science↗
USGS2026

Evaluating the effects of a multi-modal deterrent on upstream passage by invasive carp and native species

Invasive carp, including Silver Carp ( Hypophthalmichthys molitrix ) and Grass Carp ( Ctenopharyngodon idella ), are increasing their range within the Mississippi River Basin, threatening native fishes and ecosystem function. We conducted a 3-year field test of a multi-modal deterrent that uses sound, bubbles, and light to test its ability to impede upstream passage by invasive carp. The test was performed at a lock and dam on the Cumberland River, Kentucky, USA, using acoustic telemetry to track the movements of Silver Carp, Grass Carp, and several native fish species. When the deterrent was operating, the estimated probability of upstream lock passage was 53% lower (95% CI: 41%–63%) for Silver Carp but 74% higher (95% CI: 3%–190%) for Grass Carp compared to times when the deterrent was not operating. Paddlefish ( Polyodon spathula ) passage was reduced by 43% (95% CI: 71% reduction to 9% increase), although this was not statistically significant. We detected no significant effects on Freshwater Drum ( Aplodinotus grunniens ) or Smallmouth Buffalo ( Ictiobus bubalus ). However, sample sizes were small for these native species, limiting our ability to identify effects. Water temperature, tailwater elevation, and vessel lockages affected passage rates, although this varied among species.

Kentucky↗
USGS2026

Dynamic drainage reorganization in Eastern Tibet: Insights from the Yangtze River first bend

The modern drainage network of eastern Tibet is widely believed to have developed through a series of river capture and flow reversal events; however, the timing and mechanisms driving this reorganization remain contentious. Among these events, the river capture that formed the First Bend of the Yangtze River (YFB) stands out as both iconic and particularly debated. Here we present sedimentary provenance data from the Late Miocene–Quaternary Dali Basin, located south of the YFB, which indicate that a southward-flowing Jinsha River (i.e., the present-day upper Yangtze River) sourced sediment to the Dali basin at ∼7.4–6.4 Ma in a drainage configuration different from that of today. Because this interval postdates the initial establishment of a near-modern Jinsha River system prior to the Miocene, our results imply at least two discrete fluvial reorganizations occurred at the YFB—one preceding ∼7.4 Ma and another following ∼6.4 Ma. By integrating these findings with landscape evolution modeling, we infer that the initiation of rapid uplift of the Yulong-Haba Mountains and the Diancang Shan may have been responsible for these drainage reorganizations. These results underscore that Cenozoic drainage systems on the eastern Tibetan Plateau have evolved dynamically on a short timescale of ∼10 5 –10 6 -year, rather than remaining in a long-term stationary configuration on ∼10 7 -year timescales.

eastern Tibetan Plateau, first bend of the Yangtze↗
USGS2025

Characterizing the scale of regional landslide triggering from storm hydrometeorology

Rainfall strongly affects landslide triggering; however, understanding how storm characteristics relate to the severity of landslides at the regional scale has thus far remained unclear, despite the societal benefits that would result from defining this relationship. As mapped landslide inventories typically cover a small region relative to a storm system, here we develop a dimensionless index for landslide-inducing rainfall, A * , based on extremes of modeled soil water relative to its local climatology. We calibrate A * using four landslide inventories, comprising over 11 000 individual landslides over four unique storm events, and find that a common threshold can be applied to estimate regional shallow-landslide-triggering potential across diverse climatic regimes in California (USA). We then use the spatial distribution of A * , along with topography, to calculate the landslide potential area (LPA) for nine landslide-inducing storm events over the past 20 years, and we test whether atmospheric metrics describing the strength of landfalling storms, such as integrated water vapor transport, correlate with the magnitude of hazardous landslide-inducing rainfall. We find that although the events with the largest LPA do occur during exceptional atmospheric river (AR) storms, the strength of landfalling atmospheric rivers does not scale neatly with landslide potential area, and even exceptionally strong ARs may yield minimal landslide impacts. Other factors, such as antecedent soil moisture driven by storm frequency and mesoscale precipitation features within storms, are instead more likely to dictate the patterns of landslide-generating rainfall throughout the state.

California↗
USGS2025

Machine learning provides reconnaissance-type estimates of carbon dioxide storage resources in oil and gas reservoirs

Oil and gas reservoirs represent suitable containers to sequester carbon dioxide (CO 2 ) in a supercritical state because they are accessible, reservoir properties are known, and they previously contained stored buoyant fluids. However, planners must quantify the relative magnitude of the CO 2 storage resource in these reservoirs to formulate a comprehensive strategy for CO 2 mitigation. Even reconnaissance-type estimates of CO 2 storage resources of known oil and gas reservoirs may require complicated calculations involving 1) estimates of recoverable oil and gas, 2) reservoir properties (depth, temperature, pressure, etc.), and 3) the physical qualities of the retained fluids. We demonstrate the application of machine learning (ML) algorithms to bypass these computations to yield more rapid estimates of CO 2 storage resources in reservoirs capable of hosting CO 2 in a supercritical state. ML algorithms are computationally efficient because they do not impose the strong assumptions on the data-generating process that standard statistical or engineering procedures require. Further, ML algorithms can capture highly complex, particularly nonlinear, relationships among predictor variables. We demonstrate the application of four different ML algorithms using data from onshore and offshore oil and gas reservoirs in Europe, and show they perform well when predictions are compared to engineering estimates. The proposed methods and models provide an effective and novel way to more rapidly and directly determine the subsurface CO 2 storage capacity of oil and gas reservoirs around the world, information that operators, researchers, and policymakers alike require to meet energy transition and decarbonization goals.

Frontiers in Enviornmental Science↗
USGS2024

The dominance and growth of shallow groundwater resources in continuous permafrost environments

Water is a limited resource in Arctic watersheds with continuous permafrost because freezing conditions in winter and the impermeability of permafrost limit storage and connectivity between surface water and deep groundwater. However, groundwater can still be an important source of surface water in such settings, feeding springs and large aufeis fields that are abundant in cold regions and generating runoff when precipitation is rare. Whether groundwater is sourced from suprapermafrost taliks or deeper regional aquifers will impact water availability as the Arctic continues to warm and thaw. Previous research is ambiguous about the role of deep groundwater, leading to uncertainty regarding Arctic water availability and changing water resources. We analyzed chemistry and residence times of spring, stream, and river waters in the continuous permafrost zone of Alaska, spanning the mountains to the coastal plain. Water chemistry and age tracers show that surface waters are predominately sourced from recent precipitation and have short (<50 y) subsurface residence times. Remote sensing indicates trends in the areal extent of aufeis over the last 37 y, and correlations between aufeis extent and previous year summer temperature. Together, these data indicate that surface waters in continuous permafrost regions may be impacted by short flow paths and shallow suprapermafrost aquifers that are highly sensitive to climatic and hydrologic change over annual timescales. Despite the lack of connection to regional aquifers, continued warming and permafrost thaw may promote deepening of the shallow subsurface aquifers and creation of shallow taliks, providing some resilience to Arctic freshwater ecosystems.

Proceedings of the National Academy of Sciences↗
USGS2026

Hazard potential of compound flooding from rainfall, storm surge, and groundwater in coastal New York and Connecticut

Compound flood events, the co-occurrence of multiple flood drivers, can result in flood hazard potential exceeding that of any single driver alone. To evaluate compound flooding in a semi-urbanized coastal area, historical records dating back to 1970 are used to study the co-occurrences of high precipitation, storm surge, and shallow groundwater conditions along the coastlines of New York and Connecticut. Joint return periods for coincident precipitation-surge events were computed using statistical dependence models and compared to the assumption of independence as a ratio, referred to here as a return period adjustment. Results indicate distinct seasonality where compound events in the area disproportionately occur in the cold season between October and April. Return period adjustments range from a factor of 1 to almost 9, demonstrating the range in precipitation-storm surge dependence across the study area. Across all 24 station triad locations, groundwater levels were elevated during times of precipitation- surge co-occurrence, reflecting the tendency for coastal storms and shallow groundwater conditions to co-occur seasonally. The result is a pseudo-trivariate compound flood hazard score and corresponding hazard map that integrates dependence between daily precipitation-surge events and overall monthly groundwater levels (as a precondition) into a relative compound hazard score. The location with the highest compound flood hazard score is on the south shore of Long Island, as well as locations across coastal Connecticut where groundwater levels compound the co-occurrence of heavy precipitation and storm surge.

Connecticut, New York↗
USGS2025

Do bighorn sheep use desert tortoise burrow spoil piles as mineral licks in southern California?

In a previous study, bighorn sheep ( Ovis canadensis ) were periodically photographed by trail cameras when they visited desert tortoise ( Gopherus agassizii ) burrows in the San Bernardino Mountains near Palm Springs, California, USA. The authors suggested that bighorn sheep may utilize the excavated spoil material from burrows as a mineral lick. To test that supposition, we collected soil samples from the spoil piles and undisturbed surface soil up to 1 m away at two desert tortoise study sites with bighorn sheep at the aforementioned site and another nearby site. We hypothesized that sodium concentrations would be greater in spoil than in nearby surface soil samples. Common major cations were quantified in soil samples using inductively coupled plasma-mass spectrometry following microwave-assisted digestion to solubilize the fraction of elements that could become biologically or environmentally available. Mean sodium concentrations were greater in burrow spoil samples than in undisturbed soil samples at both sites, likely due to aqueous leaching of sodium from surface to subsurface soil horizons. Although we did not collect evidence of geophagy by bighorn sheep, the behavior is well known. However, trail camera photographs of bighorn sheep with their head next to the entrance of tortoise burrows suggests that they may periodically utilize sodium and other elements brought to the surface by desert tortoises that are otherwise unavailable to bighorn sheep on the surface.

California↗
USGS2026

Deep critical zone controls on shallow landslides

The deep critical zone (CZ) has long been recognized for its importance in influencing shallow landslides but was not considered feasible to include in slope stability models at the watershed scale. In this study, we demonstrate that simple approximations of the CZ in a fully coupled hydrologic and soil slope stability model can effectively capture the location, timing, and likely size of shallow landslides. To achieve this, we use coupled, process-based models that incorporate the effects of 1) deep CZ structures, 2) three-dimensional transient hydrology, and 3) multidimensional slope stability, calibrated with data from an intensively monitored field site. Our results show that the hydrologically active deep CZ guides groundwater flow, influencing where it drains from or exfiltrates to the soil mantle, producing distinct patterns of soil saturation and seepage forces at the soil-bedrock boundary. Deep conductive weathered critical zone drains the soil mantle, reducing the likelihood of destabilizing pore pressures, while the downslope thinning of the CZ forces groundwater to the surface. This creates localized instability and a tendency for similar-sized landslides across the landscape. In contrast, the absence of conductive weathered bedrock results in more widespread destabilizing pore pressures, leading to larger landslides and the likelihood of landslides earlier in a storm than in landscapes underlain by a deep CZ. Our findings suggest that first-order variations of deep CZ can provide physical explanations for variations observed in the susceptibility, magnitude, and timing of shallow landslides, and that CZ structure may be inferred from patterns and timing of landsliding.

Proceedings of the National Academy of Sciences↗
USGS2026

Hydrologic variability drives environmental and geospatial relationships in Smallmouth Bass (Micropterus dolomieu) distribution

Hydrologic variation is a primary driver of stream ecosystems. Changing hydrology can lead to assemblage shifts and alterations in suitable habitat for freshwater species. As climate change is predicted to alter flow patterns in addition to increasing water temperatures, insight into relationships between species occupancy, hydrology, and temperature is critical for understanding current and future distributions. We examined how hydrologic variability, temperature, and other environmental variables interact to influence Micropterus dolomieu (Smallmouth Bass) occurrence. We used Spatial Stream Network models, allowing for the incorporation of spatial autocorrelation along streams' unique dendritic network, to examine Smallmouth Bass occupancy across a range of hydrologic variation in the Ozark-Ouachita Interior Highlands, USA. Hydrologic variation was the main driver of Smallmouth Bass occurrence, with occurrence more likely in groundwater streams with low hydrologic variation and high flow permanence. For groundwater streams, occurrence was positively associated with summer stream temperature and negatively associated with annual stream temperature. As variation increased, more variables showed significant relationships with occurrence. Distance metrics were important for all models, however as hydrologic disturbance increased, flow connected distance played a lesser role and stream distance played a greater role. Hydrologic variability was the overarching determinant of Smallmouth Bass occurrence and strongly influenced the predictive importance of environmental variables and geospatial relationships. Greater hydrologic variability resulted in stronger statistical relationships between occurrence and environmental variables and an increased importance of system connectivity. As climate change alters hydrologic processes and streams become more variable, understanding and accounting for these shifting relationships is essential.

Arkansas, Kansas, Missouri, Oklahoma↗
USGS2026

Thermal detectability of subsurface water ice on Mars: A comparative analysis for the Subsurface Water Ice Mapping (SWIM) Project

We have developed a new global map of shallow ground ice distribution, SWIM23, based on Mars Global Surveyor Thermal Emission Spectrometer data and made systematic comparisons between this new map and two similar, previously developed data products. We have explored the origin of differences between the three ice maps by detailing technical and procedural differences in their development, by making global pixel-by-pixel comparisons, and by carrying out a series of one-dimensional thermal simulations to explore fundamental physical limitations of thermal ice-detection techniques. These efforts and the production of a composite thermal ice-consistency map supported integration of multiple geophysical data products relevant to ice detection in the upper meter of the Martian regolith into the larger Mars Subsurface Water Ice Mapping project. Our work also highlights fundamental physical limitations to thermal ice detection as a technique, particularly the rapid fall-off in ice detection sensitivity at depths >30 cm, which produces maximum uncertainty in the presence and depth of ice within regions preferred for potential human landing sites. A future Mars orbiter mission designed to detect ice and support crewed landing site selection in the midlatitude region should give payload priority to an instrument capable of probing the 1–5 m depth range (i.e., a high-frequency radar), over a next-generation thermal spectrometer, which is unlikely to offer clarity on ice table depths or lateral continuity of the ice table in the locations of highest interest.

Planetary Science Journal↗
USGS2025

How do hydrological variability and human activities control the spatiotemporal changes of riverine nitrogen export in the Upper Mississippi River Basin?

Excessive nitrogen export from agricultural watersheds remains a critical water quality challenge, with the Upper Mississippi River Basin (UMRB) significantly contributing to downstream eutrophication and hypoxia in the Gulf. This study investigates the spatiotemporal dynamics of riverine nitrate plus nitrite (NO 3 – + NO 2 – -N) export across the UMRB at high spatial resolution (12-digit Hydrologic Unit Codes or HUC12 subwatershed scale) during 2001–2020 and quantifies the effects of anthropogenic activities and hydrological variability on riverine NO 3 – + NO 2 – -N export changes in the region between 2001–2005 and 2016–2020. Our results revealed hotspots of substantial increases in NO 3 – + NO 2 – -N yields across the UMRB, with distinct regional patterns in driving factors. Over the entire UMRB, NO 3 – + NO 2 – -N yields increased by 9.7 kg/ha/yr on average from 2001–2005 to 2016–2020, with anthropogenic activities contributing 4.8 kg/ha/yr and hydrological variability contributing 4.9 kg/ha/yr. The northern and western UMRB had combined influences from both anthropogenic activities and hydrological variability, while the east-central regions had predominantly hydrologically driven changes. Agricultural sources, including fertilizer, manure, and biological nitrogen fixation, collectively contributed over 80% of NO 3 – + NO 2 – -N loading throughout the basin. This framework for disentangling human and hydrological impacts provides critical insights for developing effective and targeted watershed management strategies to reduce nutrient losses and improve water quality.

Upper Mississippi River Basin↗
USGS2024

Perspectives on transportable array Alaska background noise levels

Background seismic noise fundamentally sets a lower bound on our ability to record signals arising from earthquakes. The background noise spectrum at a station is a combination of cultural noise, ocean-generated microseism noise, intrinsic instrument self-noise, and the sensitivity of the instrument to nonseismic noise sources. The USArray-Transportable Array Alaska deployed 195 stations across Alaska and parts of Canada (Yukon, British Columbia, and Northwest Territories). These stations were all installed using similar techniques and made use of instruments with similar self-noise levels. As such, this network provides an opportunity to look at how geographic location influences seismic background. Using these broadband stations, we report background noise levels from 0.2 to 75 s period in six discrete bands. By constructing “noise maps,” we depict both spatial and temporal changes in the background noise field. Using these maps, combined with targeted analysis, we infer sources and contributing factors to noise levels in these different period bands. These include cultural noise, the formation of sea ice, seasonal changes in permafrost and wave activity in the Gulf of Alaska, and magnetic field variability. We use this study as an opportunity to review several previous studies examining seismic noise in Arctic regions.

Book chapter↗
USGS2024

Seismicity zoning at Coso geothermal field and stress changes from fluid production and migration

The Coso geothermal field is a major geothermal power production site in the western United States. It has been observed that low-magnitude seismic events ( M < 3.71) are unevenly distributed in three distinct zones, namely, nearfield (<3 km), midfield (3–6 km), and farfield (> 6 km) from the Coso geothermal plant. These zones exhibit distinct changes in earthquake location before and during geothermal production episodes that began in 1986. After 1986, the midfield region of the main flank experiences a significantly lower seismicity rate than the surrounding areas before production episodes. During 2014–2019, the farfield earthquakes cluster in the eastern and western parts of the greater Coso area, which is discernably different from how those pre-production earthquake events were distributed along the conjugate NW-SE and SW-NW trending structures across the main flank. Here, we analyze the stage of stress with finite-element-based poroelastic simulations to illustrate how the spatiotemporal evolution of the seismicity is associated with the pattern of stress perturbations caused by fluid migration amid the operations of geothermal power plants. Generally, ∼70% of co-production seismicity is found in zones of increased Coulomb stress between 2014 and 2019 at >99% confidence. Meanwhile, the midfield zone of seismic paucity overlaps with the zone of decreasing pore-fluid pressure. Overall, the results provide a physical explanation of how decadal geothermal operations at Coso have perturbed stress-field changes and contributed to the evolving characteristic seismic pattern, shedding insights into assessing the seismic hazard in other geothermal settings.

California↗
USGS2026

Widespread terrestrial ecosystem disruption at the onset of the Paleocene–Eocene Thermal Maximum

The Paleocene–Eocene Thermal Maximum (PETM, ~56 Mya) interval was marked by massive 13 C-depleted carbon emissions into the ocean/atmosphere system, manifested as a negative carbon isotope excursion (CIE) in sedimentary components, and ~5 °C global average warming. Episodes of hydrological perturbations and soil-erosion have been widely documented for the PETM but their link with vegetation- and carbon cycle changes remain poorly constrained. Here, we present organic microfossil evidence showing a strong increase in fern-dominated pioneer vegetation that replaced coniferous forests on the margin of the Norwegian Sea during the first millennia of the CIE. With the present stratigraphic constraints, the “fern spike” occurred simultaneously in terrestrial settings along the North Sea, Arctic Ocean, the US east coast and in southern Australia, indicating that pioneer vegetation persisted for several millennia following a partial collapse of previously stable terrestrial ecosystems. Both the ferns and influx of microcharcoal imply recurrent physical disturbance, including soil destabilization and erosion, potentially linked to droughts, wildfires, and strong hydrological forcing resulting from extreme climate change. Together with evidence for reworked clay minerals and ancient organic matter (kerogen), these findings show that highly disturbed terrestrial ecosystems were widespread across mid- and high-latitude regions globally. Carbon cycle model simulations suggest that a substantial loss of standing and buried biomass, along with oxidation of soil organic matter, acted as important positive feedbacks during the onset of the CIE. Additionally, enhanced kerogen weathering likely contributed as another major positive feedback throughout both the onset and main phase of the CIE.

Proceedings of the National Academy of Sciences↗
USGS2026

PFAS mixture composition and internal exposure profiles shape biological responses under field-realistic exposure

Per- and polyfluoroalkyl substances (PFAS) occur as complex mixtures, yet mixture-dependent biological effects under environmentally realistic exposure conditions remain poorly understood. We conducted multiyear (2018, 2019, 2021) continuous-flow, field-based exposures of male fathead minnows ( Pimephales promelas ) using a low-PFAS reference well (REF; sum of measured PFAS (∑PFAS) 0.1–0.2 μg L –1 ) and PFAS-contaminated groundwater from a fire-training area (FTA) at Joint Base Cape Cod, Massachusetts. Dilution treatments enabled separation of concentration and mixture effects. Groundwater from well FTA1 was perfluorooctanesulfonate (PFOS) dominated (∑PFAS 10–31 μg L –1 ), whereas groundwater from well FTA2 had higher concentrations and was enriched in perfluorooctanoate (PFOA) and diverse precursors (∑PFAS ∼ 80 μg L –1 ). Despite comparable plasma ∑PFAS and PFOS in FTA1–100% and FTA2–50% on day-7, cumulative mortality reached approximately 35% in FTA2–50% and 17% in FTA2–100%, and secondary sex trait expression was reduced by approximately 65–80% relative to REF, whereas sperm motility effects were mixture- and time-dependent. Plasma PFAS profiles were dominated by perfluorohexanesulfonate (PFHxS), PFOS, and sulfonamide precursors. Liver transcriptomics from REF-acclimated fish revealed robust disruption of metabolic, mitochondrial, and endocrine pathways that link PFAS mixture chemistry and internal exposure profiles to organismal outcomes, and testis transcriptomics provided complementary insight into reproductive impairment. These results indicate that PFAS mixture composition and internal exposure profiles, including precursor-associated differences, are important determinants of ecotoxicological outcomes under field-realistic conditions.

Massachusetts↗