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USGS2026

Decadal-scale characteristics of natural and anthropogenic dune morphology along North Carolina Barrier Islands (SE Atlantic Coast)

Dunes offer protection to coastal communities from storms, yet quantitative research characterizing human influences on dune systems across decades and 10–100 km spatial scales constitutes a known data gap. This study analyzed a multidecadal archive of dune crest elevations and positions to map mesoscale spatiotemporal trends in foredune morphology across the barrier island coastline of North Carolina, SE Atlantic coast. Results are summarized within four categories of coastline representing a range of coastal management intensities, integrating beach width and dune lateral accommodation space to assess the comparative influence of differing intensities of anthropogenic intervention on dune morphology. These categories account for the presence of structures, roads, and alongshore connectivity to anthropogenic sediment sources (beach nourishment). These categories show distinct dune and beach system characteristics. Dunes are largest in the areas with the longest legacy of coastal management. As categories decrease in development intensity, dune and beach system characteristics generally depart from the large, static dunes and narrow beaches consistent with hold-the-line management. Where small or transgressive dunes exist on heavily developed coasts, they coincide with relatively strong hydrodynamic forcing or sediment-limiting geology. There is evidence of indirect coastal management influence on undeveloped barrier islands with alongshore connectivity to developed barriers. While this analysis was conducted at the mesoscale, the results provide researchers with the framework to make insights at smaller scales than those demonstrated in this study.

North Carolina↗
USGS2026

Assessing future hydrologic extremes using an integrated hydrology and river operations model in the Russian River watershed

Study region The Russian River watershed, situated in coastal, northern California, experiences hydrologic extremes, including periodic droughts and flooding. Water managers are working to maintain sustainable water supplies and environmental flows, while mitigating flood risks. Study focus This paper introduces an integrated hydrology and river operations model for the Russian River watershed. This model is distinct from models in previous studies because it represents surface-groundwater interactions and uses climate forcings to estimate dynamic water use demands that are superimposed onto both reservoir operations and water supply constraints. The model was used to examine three historical (1990–2015) and eight future (2016–2099) water use and climate change scenarios. New hydrological insights for the region The direct connection between streams and aquifers facilitated both annual aquifer replenishment by high winter streamflows and streamflow depletion by groundwater wells (19 % of pumped groundwater in alluvial aquifers from stream leakage) during critical low flow periods. Simulated streamflow changes included 59 % longer and 54 % more severe streamflow droughts, 26 % lower seasonal low streamflows, and up to 125 % higher peak streamflows, averaged over future climate and water use scenarios, suggesting increased future flood and water availability risks. Results showed the importance of reservoir operations for mitigating the impacts of increased hydroclimatic volatility, despite a decrease in reservoir reliability at Lake Mendocino, suggesting that reservoir management may be used to decrease future risks.

California↗
USGS2026

Evaluating the U.S. Geological Survey’s earthquake shaking hazard forecasts and their implications on seismic risk

We analyze the last six update cycles of the long-term probabilistic earthquake shaking hazard forecast from the U.S. Geological Survey (USGS) and discuss the changes in hazard estimates from the 1996 to the latest 2023 update of the National Seismic Hazard Model (NSHM) for the conterminous U.S. We summarize how our understanding of earthquake hazards has evolved over the last two decades and quantify the implications of changing hazards on people, buildings, and lifeline infrastructure. The net effect of changes in hazard estimates, with reference to the mean hazard from the 2023 NSHM update that has 2% probability of exceedance in 50 years, suggests an overall increase in total geographic areas facing very strong shaking levels (modified Mercalli Intensity (MMI) of VII or more) when compared with any of the previous cycles. This increase puts ~159.2 million people (an increase from 111.8 million when using the 2018 NSHM), ~47.3 million residential buildings (an increase from 32.5 million with the 2018 NSHM), and ~$25.6 trillion of economic exposure of all buildings (an increase from $17.4 trillion when using the 2018 NSHM) at risk from earthquakes. We demonstrate that small changes in hazard estimates do not necessarily imply small changes in risk estimates. Therefore, changes in risk estimates can be used to highlight key USGS NSHM updates for improving risk mitigation efforts.

Earthquake Spectra Journal↗
USGS2025

Hydrologic variability and groundwater age of springs in eastern Oregon and northern Nevada, USA

The ecological importance of springs in semiarid regions is far greater than their small size and sparse distribution, yet little is known about the hydrologic functioning of these systems. During 2016–22, 261 springs were visited in the volcanic terrane of eastern Oregon and northern Nevada. When conditions were suitable, measurements of discharge, water temperature, and specific conductance were made, and samples for the analysis of carbon-14, tritium, and water stable isotopes (WSI) were collected. A subset of 60 springs was revisited during different seasons in the same year and during the dry season in multiple years to evaluate variability in discharge, chemistry, and groundwater age. Specific conductance and WSI varied considerably among springs across the study area but were unexpectedly stable across seasons and years at individual springs. Seasonal and interannual variability in spring discharge was related to the residence time of the discharging groundwater. Springs discharging older groundwater (10 3 –10 4 years) had significantly less variability in their discharge compared to springs discharging younger groundwater (10 0 –10 1 years). Variability among springs discharging younger groundwater included cessation of late-summer discharge at 18 % of the repeat-visit springs. A logistic regression model predicted the age of discharging spring water with 89 % accuracy using only the spring latitude, longitude, elevation, and δ 2 H value. This study framework provides a simple, inexpensive, and robust method to provisionally assess the hydrologic behavior of springs having little or no prior information in understudied, semiarid regions across the globe.

Nevada, Oregon↗
USGS2025

Assessing earthquake risks to lifeline infrastructure systems in the United States

The security and economic stability of the United States rely heavily on robust lifeline infrastructure systems and yet the risks to such systems are seldom quantified at the national scale. For example, while earthquake risks to buildings in the United States have been investigated at the national scale regularly, such risks to gas pipelines have rarely been investigated nationally. In this paper, we use examples from two critical infrastructure sectors to demonstrate (1) the nature of earthquake risks to lifeline infrastructure systems, (2) complexities involved in regional seismic risk assessments, and (3) how such risks change with time. We found that bridge risks can be underestimated by at least 64 % when viewed from repair costs instead of traffic demands and that regional risks can be underestimated by 19 % when spatial correlations of ground motion are ignored. Further, exceedance of traffic demand can be 50 times more likely to occur when viewed at the regional scale than when viewed at an individual bridge. Similarly, exceedance of repairs can be 180 times more likely to occur when viewed at the pipeline network level than at a segment-specific level. Finally, sensitivity analyses with the 2018 and 2023 USGS National Seismic Hazard Models indicate an increase in bridge risk of at least 24 % and an increase in exposed gas pipeline mileage of 43 %. The evolution of risks, complexities involved in assessments, and limited resources jointly underscore the need for more routine updates to nationwide seismic risk assessments of lifeline systems in the United States.

International Journal of Critical Infrastructure P↗
USGS2026

Ringtail (Bassariscus astutus) survival in southwestern Oregon

The effective conservation and management of small carnivore populations requires understanding species’ life-history traits and identifying important vital rates that drive population trajectories. However, many of these species are rare or elusive and of state or federal conservation concern, and demographic information is often lacking and difficult to obtain. At the northern limit of their range in Oregon, USA, ringtail ( Bassariscus astutus ) occupy mid-elevation forests and are a species of conservation concern because of their limited distribution and suspected low density. We initiated a radio-telemetry study in 2020 to estimate monthly and annual survival of ringtail in southwest Oregon. We monitored 26 ringtail from November 2020 to October 2022 and estimated survival rates using a known-fate framework and Program MARK. Model-averaged monthly survival estimates ranged from lows of 0.963 (SE = 0.022, 95% CI = 0.887–0.988) in spring (Feb–May) of 2021 to highs of 0.980 (SE = 0.018, 95% CI = 0.889–0.997) in summer (Jun–Oct) of 2022. Model-averaged estimates of annual survival were 0.695 (SE = 0.176, 95% CI = 0.310–0.920) during 2020–2021 and 0.728 (SE = 0.168, 95% CI = 0.336–0.934) during 2021–2022. Predation was the leading cause of mortality, but notably, no mortalities could be attributed to avian predation. Survival rates were much higher within forested landscapes in Oregon than for populations in the southwestern United States, which may have implications for regional conservation and management strategies.

Oregon↗
USGS2025

Per- and polyfluoroalkyl substances (PFAS) mass flux and mass balance at an aqueous film-forming foam release site in semiarid eastern New Mexico, USA

Passive flux meters (PFMs) directly measure groundwater chemistry mass flux and Darcy flux, providing insight into contaminant source-zone architecture and transport properties. This study uses PFMs to characterize PFAS flux in groundwater at a semiarid site with a thick (greater than 90-m) unsaturated zone where groundwater has been contaminated with per - and polyfluoroalkyl substances (PFAS) related to the use of aqueous film-forming foam (AFFF) for fire training and fire suppression. PFAS mass discharge (PFAS mass flux integrated over a control plane) in groundwater downgradient from several PFAS release areas is calculated using PFM results. In groundwater downgradient from fire-training areas, total PFAS mass discharge (summed across 14 compounds) was estimated to be between 6.0 and 31 g per day in 2020 and between 5.9 and 23 g per day in 2021. Site-specific documentation, generic information on AFFF properties, and literature values of PFAS concentration in AFFF are used to estimate site-specific PFAS-application rates at fire-training areas. These PFAS-application rates are compared to groundwater PFAS-discharge rates. Results suggest that transformation processes (exact pathways unknown) have led to increased discharge of measured PFAS in groundwater relative to initial AFFF formulations. The mass balance approach has broad applicability as a high-level approach that can provide insight into PFAS transport at AFFF sites.

New Mexico↗
USGS2026

Evaluating the effects of a multi-modal deterrent on upstream passage by invasive carp and native species

Invasive carp, including Silver Carp ( Hypophthalmichthys molitrix ) and Grass Carp ( Ctenopharyngodon idella ), are increasing their range within the Mississippi River Basin, threatening native fishes and ecosystem function. We conducted a 3-year field test of a multi-modal deterrent that uses sound, bubbles, and light to test its ability to impede upstream passage by invasive carp. The test was performed at a lock and dam on the Cumberland River, Kentucky, USA, using acoustic telemetry to track the movements of Silver Carp, Grass Carp, and several native fish species. When the deterrent was operating, the estimated probability of upstream lock passage was 53% lower (95% CI: 41%–63%) for Silver Carp but 74% higher (95% CI: 3%–190%) for Grass Carp compared to times when the deterrent was not operating. Paddlefish ( Polyodon spathula ) passage was reduced by 43% (95% CI: 71% reduction to 9% increase), although this was not statistically significant. We detected no significant effects on Freshwater Drum ( Aplodinotus grunniens ) or Smallmouth Buffalo ( Ictiobus bubalus ). However, sample sizes were small for these native species, limiting our ability to identify effects. Water temperature, tailwater elevation, and vessel lockages affected passage rates, although this varied among species.

Kentucky↗
USGS2025

Linking stream-reach nitrogen loads and groundwater “reachsheds” to inform wastewater-nitrogen management actions, Cape Cod, Massachusetts

Study Region Cape Cod, Massachusetts, U.S.A. Study Focus Anthropogenic nitrogen (N) is a key factor in degrading groundwater and surface-water quality, particularly in coastal New England where onsite wastewater systems are prevalent. This study evaluated whether direct N-load measurements in streams on Cape Cod, Massachusetts, coupled with flow-path information from groundwater-flow models, can effectively identify potential land areas where nitrogen mitigation could substantially reduce loads to receiving waters. Nitrogen fluxes were measured along 63 stream reaches during winter and summer and paired with simulated groundwater recharge areas to identify and rank potential areas for reduction of nonpoint-source N inputs. New Hydrologic Insights for the Region Reach-scale nitrate-N loads ranged from −39.1–1182 kg-N/yr per 100 m of stream, indicating spatially variable groundwater inputs across seasons. “Reachsheds” — areas contributing groundwater recharge to specific stream reaches — were delineated using a regional groundwater-flow model. Strong correlations were found between observed N loads and land-use characteristics, especially the number of septic systems and total N inputs from the sum of considered sources. Observed N loads were moderately correlated with recharge area size and wastewater flow estimates. Correlating reach-specific groundwater N loads with land use and parcel-scale nitrogen-yield data identified reachsheds with the highest potential for N load reduction. This approach enables targeted implementation of restoration efforts to optimize nutrient management and support regional load reduction.

Massachusetts↗
USGS2026

Surface-wave relocation and characterization of the 2024–2025 dyking episode along the Fentale–Dofen segment of the Ethiopian rift

From late-December 2024 to mid-March 2025, a 50-km-long dyke intrusion triggered over 300 earthquakes (magnitude 4 to 5.9) between Fentale and Dofen volcanoes along the Northern Main Ethiopian Rift. Dyke intrusions periodically occur along the Fentale–Dofen magmatic segment and are an expression of ongoing rift extension. Preliminary analyses using interferometric synthetic aperture radar revealed extensive ground deformation (up to 60 cm), which closely matched the temporal and spatial evolution of surface manifestations and earthquake locations from global catalogues. While global catalogues are critical for real-time monitoring, the precision of locations in remote and or sparsely instrumented regions can be low. In this investigation, we present surface-wave relocation results of the dyking episode that began near Fentale volcano in December 2024. We estimate relative locations using differential traveltimes measured from regional-to-teleseismic distance surface-wave observations of earthquakes reported by the U.S. Geological Survey. Relative relocations reduce the initial region of diffuse seismicity to a 50-km-long narrow band bounding the strike of surface manifestations and the zone of maximum surface deformation. We demonstrate the precision of surface-wave relocations over incremental time periods, capturing the progression of dyking from seismic onset through seismic migration and caldera subsidence. Results showcase the utility of surface-wave relocations in the characterization of dyking episodes and provide complementary insights into the current understanding of the Fentale–Dofen volcanic plumbing system.

Geophysical Journal International↗
USGS2023

Slow slip detectability in seafloor pressure records offshore Alaska

In subduction zones worldwide, seafloor pressure data are used to observe tectonic deformation, particularly from megathrust earthquakes and slow slip events (SSEs). However, such measurements are also sensitive to oceanographic circulation-generated pressures over a range of frequencies that conflate with tectonic signals of interest. Using seafloor pressure and temperature data from the Alaska Amphibious Community Seismic Experiment, and sea surface height data from satellite altimetry, we evaluate the efficacy of various seasonal and oceanographic pressure signal proxy corrections and conduct synthetic tests to determine their impact on the timing and amplitude prediction of ramp-like signals typical of SSEs. We find that subtracting out the first mode of the complex empirical orthogonal functions of the pressure records on either the shelf or slope yields signal root-mean-square error (RMS) reductions up to 73% or 80%, respectively. Additional correction with proxies that exploit the depth-dependent spatial coherence of pressure records provides cumulative variance reductions up to 83% and 93%, respectively. Our detectability tests show that the timing and amplitude of synthetic SSE-like ramps can be well constrained for ramp amplitudes ≥4 cm on the shelf and ≥2 cm on the slope, using a fully automated detector. The principal limits on detectability are residual abrupt changes in pressure that occur as part of the transition to and from summer to winter conditions but are not adequately characterized by our seasonal corrections, as well as the inability to properly account for instrumental drift, which is not readily separated from the seasonal signal.

Alaska↗
USGS2025

Uncertainty reduction for subaerial landslide-tsunami hazards

Subaerial rock slopes may generate a tsunami by rapidly moving into the water. Large uncertainty in landslide characteristics propagates into large uncertainty in tsunami hazard, making hazard assessment more difficult for land and emergency managers. Once a potentially tsunamigenic landslide is identified, it may not be clear which landslide characteristics contribute most significantly to uncertainty in the tsunami hazard. Our aim is to document the relative worth of different landslide characteristics (e.g., size, material properties) for reducing uncertainty in landslide-tsunami hazard assessments. Isolating the relative importance of specific landslide characteristics may inform prioritization of data collection and improve efficiency in understanding hazard. To accomplish this, we generated a set of 288 landslide-tsunami simulations in which we systematically varied the size and material properties of possible failure extents at the Barry Arm landslide complex in northwestern Prince William Sound, Alaska, USA. We find that for landslides smaller than the receiving waterbody, the landslide volume has the strongest effect on resulting wave characteristics and thus the highest leverage on reducing uncertainty in tsunami hazard. In contrast, for landslides substantially larger than the waterbody, the duration of rapid movement of the landslide has the highest leverage. Based on our results, we propose a classification scheme for subaerial landslides based on the relative size of the landslide and waterbody. Additionally, our results support the generation of a tsunami height transfer function between existing tide gages and a nearby coastal city. These results have direct implications for the practice of operational early warning.

Alaska↗
USGS2025

Estimated average annualized losses from potential building damage and fatalities due to earthquake-generated tsunamis in the United States

Earthquake-generated tsunamis represent substantial economic threats to states and territories in the United States (U.S.), but we are unaware of any effort to quantify potential impacts at the national level. This gap is partially due to the lack of nationally consistent data on tsunamigenic sources and associated return periods. This study addresses this issue and provides estimates of average annualized losses (AAL) for potential residential fatalities and capital stock losses associated with building damage (i.e., structural, non-structural, contents, and inventory damage) in the U.S. by curating tsunami-hazard information based on deterministic scenarios and probabilistic approaches, calculating potential losses, and estimating return periods where necessary. This assessment was done for the U.S. West Coast, Alaska, Hawaii, U.S. Pacific Territories, and U.S. Atlantic Territories. We estimate that earthquake-generated tsunamis that could affect these states and territories collectively represent $1 billion in potential AAL with 79 % of losses due to residential fatalities and 21 % of losses due to capital stock losses from building damage. We identify AAL variations based on county and county equivalents, states and territories, geographic regions, return periods, and departure-delay assumptions for evacuating residents. Results include high AAL values for potential fatalities in Puerto Rico and the U.S. Pacific Northwest region, high AAL values for potential building-related damage in Hawaii and California, and high building- and population-loss ratios for county equivalents in Alaska and U.S. territories.

International Journal of Disaster Risk Reduction↗
USGS2024

Constraining mean landslide occurrence rates for non-temporal landslide inventories using high-resolution elevation data

Constraining landslide occurrence rates can help to generate landslide hazard models that predict the spatial and temporal occurrence of landslides. However, most landslide inventories do not include any temporal data due to the difficulties of dating landslide deposits. Here we introduce a method for estimating the mean landslide occurrence rate of deep-seated rotational and translational slides derived solely from high-resolution (≤3 m) elevation data and globally available estimates of the diffusion coefficient for sediment flux. The method applies a linear diffusion model to the roughest landslide deposits until they reach a representative non-landslide roughness distribution. This estimates the time for a landslide deposit to be unrecognizable in high-resolution digital elevation data, which we term the mean lifetime of the landslide. Using the mean lifetime and number of landslides within an area of interest, we can estimate the mean occurrence rate of landslides over that domain. We validate this approach using a comprehensive temporal inventory of landslides in western Oregon created using age-roughness curves that are calibrated with high-resolution elevation data and radiocarbon data. We find good agreement between our diffusion method and the existing age-roughness-derived estimates, producing mean lifetimes of 4500 and 5200 years (4% difference), respectively. Hazard maps produced using the two methodologies generally agree, with the maximum differences in landslide probability reaching 0.1. Due to the relative abundance of high-resolution elevation data compared with age-dated landslides, our method could help constrain landslide occurrence rates in areas previously considered unfeasible.

Oregon↗
USGS2026

Egg thiamine concentrations reflect dietary differences between four hatchery strains, including two morphotypes, of Lake Trout in Lake Ontario

Objective Test whether dietary differences among hatchery strains of Lake Trout Salvelinus namaycush were associated with variation in egg thiamine concentrations and assess the potential for using strain-specific traits to mitigate thiamine deficiency complex (TDC) in Lake Ontario restoration efforts. Methods Stomach content analysis and stable isotope mixing models were used to compare diets among four hatchery strains of Lake Trout, representing lean and humper morphotypes. Egg thiamine concentrations were measured to test whether dietary differences correlated with susceptibility to TDC. Results Significant differences in diet and egg thiamine concentrations were observed among Lake Trout strains. A humper-like morphotype strain exhibited elevated thiamine levels, which were associated with reduced consumption of prey known to degrade thiamine levels when consumed, Alewife Alosa pseudoharengus and Rainbow Smelt Osmerus mordax , and increased reliance on Round Goby Neogobius melanostomus or sculpin species (Slimy Sculpin Cottus cognatus and Deepwater Sculpin Myoxocephalus thompsonii ). These findings support the link between the consumption of Alewife and Rainbow Smelt and thiamine deficiency. Conclusions Our findings support a link between strain-specific dietary preferences and susceptibility to TDC in Lake Trout. Preferential stocking of strains less prone to TDC, such as the strain derived from a humper morph from Lake Superior, could enhance restoration success in Lake Ontario. This strategy may be especially effective given the continued dominance of Alewife in the lake's prey base and the current management priorities supporting fisheries for Pacific salmon Oncorhynchus spp.

North American Journal of Fisheries Management↗
USGS2024

High resolution identification and quantification of diffuse deep groundwater discharge in mountain rivers using continuous boat-mounted helium measurements

Discharge of deeply sourced groundwater to streams is difficult to locate and quantify, particularly where both discrete and diffuse discharge points exist, but diffuse discharge is one of the primary controls on solute budgets in mountainous watersheds. The noble gas helium is a unique identifier of deep groundwater discharge because groundwater with long residence times is commonly enriched in helium. In this study, a portable mass spectrometer was used to measure longitudinal variation in dissolved helium concentrations in two mountainous rivers at high spatial resolution not feasible with traditional sampling techniques. Helium profiles were then simulated using a mass-balance model to quantify longitudinal variation in groundwater discharge to the receiving rivers. Results indicate helium concentrations were enriched by multiple orders of magnitude above atmospheric equilibrium in both rivers and that this persisted for up to 18 km below observed pulse inputs in the Colorado River. Helium mass-balance models match observed longitudinal patterns with the exception of sharp initial increases in helium observed in the rivers. Increased longitudinal groundwater discharge rates correspond to mapped geologic structures in both watersheds that likely transport deep geothermal water. Models show variable sensitivity to spatial assignment of input variables representing the groundwater source, illustrating the importance of collecting data from discrete groundwater discharges where possible. The methodology shows promise for field experiments designed to assess air–water exchange rates and to quantify total groundwater discharge from a combination of discrete and diffuse sources.

Colorado, Utah↗
USGS2025

Hydrologic response of groundwater and streamflow to natural and anthropogenic drivers of change in headwaters of the upper Colorado River basin during recent wet (1982–1999) and drought (2000–2022) conditions

Study region: Headwaters of the upper Colorado River basin (UCOL), USA Study focus: Surface-water and groundwater numerical models incorporating water-use information were used to investigate changes in climate, water use, and simulated hydrologic responses of snow processes, evapotranspiration, groundwater, and streamflow during recent wet (1982–1999) and drought (2000–2022) periods in the headwater subregions of the upper Colorado River basin. New hydrologic insights for the region: Decreases in average streamflow between wet and drought periods ranged from 20 % in the Colorado River headwaters subregion to 23 % in the Gunnison River headwaters subregion. Like streamflow, average surface runoff was statistically less during the drought than the wet period, with decreases from 24–31 % in the headwaters. On a volume basis, runoff decreases were greater than streamflow decreases in both the Colorado River and Gunnison River headwaters. Although the amount of water-year groundwater discharge to streams remained nearly the same between the wet and drought periods, groundwater as a percentage of streamflow increased between the wet and drought periods, highlighting the importance of groundwater in sustaining streamflow during drought conditions. Multiple linear regression analyses revealed that snowmelt-only models were better than the best precipitation and temperature models at explaining streamflow variability from all headwater subregions for both the wet and drought periods.

Arizona, Colorado, New Mexico, Utah, Wyoming↗
USGS2025

Manipulation of the Symbiodiniaceae microbiome confers multigenerational impacts on symbioses and reproductive ecology of its Exaiptasia diaphana host

Symbiodiniaceae-associated microbiota strongly influence cnidarian symbioses. We systematically reduced the bacterial and fungal communities associated with Symbiodiniaceae to study potential effects on the cnidarian holobiont Exaiptasia diaphana (Aiptasia). Clonal anemones were inoculated with xenic Breviolum minutum (SSB01) and microbiome-manipulated cultures after antibacterial or antifungal treatment. The asexual reproduction of pedal laceration allowed for three generations of clonal aposymbiotic Aiptasia to be utilised in this study, from the initial adult generation (G0), to the first (G1), and second (G2) generation. We inoculated small and large G1 Aiptasia with SSB01 algae and monitored onset of symbiosis, rate of algal proliferation, and holobiont characteristics. Sequencing the 16S and 18S rRNA gene regions identified significant differences in the bacterial and fungal communities of the G0 and G1 generations, alongside differences between the size classes of small and large G1 anemones. The microbiome of larger G1 individuals was distinct to the smaller G1 anemones, suggesting a microbiome maturation process. Control Breviolum minutum cultures exhibited a significantly greater proliferation rate in large G1 anemones when compared to antibacterial or antifungal treated cultures, whereas the opposite trend was documented in the small G1 anemones. Although no differences were observed between algal photochemical parameters, or the growth and polyp activity of G1 juveniles, we observed a significant influence in the production of G2 clones between treatments. Overall, we provide strong ecological implications of manipulating Symbiodiniaceae microbiome, not for the algae themselves, but for the maturation of the host Aiptasia, as well as for the cnidarian holobiont over multiple generations.

The ISME Journal↗