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USGS · 1003267

Turnover and urinary excretion of free and acetylated MS-222 rainbow trout, Salmo gairdneri

J. B. Hunn·R.A. Schoettger·W. A. Willford
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Abstract

Rainbow trout (Salmo gairdneri) anesthetized in 100 mg/liter of M.S. 222 at 12 C excreted the drug in free and acetylated forms via the urine during a 24-hr recovery period in freshwater. Of the M.S. 222 excreted, 77-96% was acetylated. Blood levels of free drug in anesthetized trout approximated 75% of the anesthetic concentration, but the amount of acetylated M.S. 222 was relatively insignificant. The blood and urine were cleared of the two fractions of M.S. 222 in 8 and 24 hr respectively. Low levels of aromatic amines of natural origin occurred in blood and urine and were subtracted from measurements of M.S. 222. Intraperitoneal injections of 10-100 mg/kg of M.S. 222 did not induce anesthesia; however, the 24-hr pattern of drug excretion was similar to that observed after anesthesia by immersion. Only 15-21 % of the injected dose was found in the urine, suggesting a second route of drug elimination.

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J. B. Hunn, R.A. Schoettger, W. A. Willford. 1968. Turnover and urinary excretion of free and acetylated MS-222 rainbow trout, Salmo gairdneri. https://doi.org/10.1139/f68-003

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