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J. B. Hunn

Publications and source records attributed to J. B. Hunn.

At least 19 recordsLinked to original sources

Renal excretion in channel catfish following injection of quinaldine sulphate or 3-trifluoromethyl-4-nitrophenol

Channel catfish, Ictalurus punctatus Rafinesque, injected intraperitoneally with 2-methyl-quinoline sulphate (QdSO 4 ) or 3-trifluoromethyl-4-nitrophenol (TFM) eliminate most of the dose of these compounds by extra-renal routes. Patterns of renal excretion of Na + , K + , Ca 2+ , Mg 2+ , and Cl - (pEq kg -1 h -1 ) appeared to be associated with the 'stress' of the urine collection technique rather than with the elimination of either compound. Concentrations of Na + , K + , Ca 2+ , Mg 2+ , and Cl - (mEq/1) were determined in urine, plasma and gall bladder bile.

Journal of Fish Biology↗

Biomonitoring of lead-contaminated Missouri streams with an assay for erythrocyte δ-aminolevulinic acid dehydratase activity in fish blood

The activity of the enzyme δ-aminolevulinic acid dehydratase (ALA-D) in erythrocytes has long been used as a biomarker of lead exposure in humans and waterfowl and, more recently, in fishes. The assay was tested for ALA-D activity in fishes from streams affected by lead in combination with other metals from lead-zinc mining and related activities. Fishes (mostly catostomids) were collected from sites affected by historic and current mining activities, and from sites considered to be unaffected by mining (reference sites). A group of potentially toxic elements was measured in blood and carcass samples of individual fish, as were ALA-D activity, total protein (TP), and hemoglobin (Hb) in blood. Concentrations of mining-related metals (lead, zinc, and cadmium) were significantly greater (P<0.05) in fish blood and carcass at sites affected by historic mining activities than at reference and active mining sites. When analyzed by multiple regression, ALA-D activity, Hb, and TP accounted for 66% of blood-lead and 69% of carcass-lead variability. Differences among species were small. ALA-D activity as a biomarker adequately distinguished sites affected by bioavailable environmental lead. Zinc was the only other metal that affected ALA-D activity; it appeared to ameliorate the inactivation of ALA-D by lead.

Archives of Environmental Contamination and Toxico↗

The use of freshwater and saltwater animals to distinguish between the toxic effects of salinity and contaminants in irrigation drain water

Irrigation drain waters entering Stillwater Wildlife Management Area (SWMA) in south-western Nevada contain elevated levels of salinity and several inorganic contaminants (As, B, Cu, Li, Mo, and Sr). Mortalities of fish and waterfowl at the management area are believed to be associated with the poor water quality of the drains. The objective of the present study was to use fresh-water and saltwater animals to distinguish between the toxic effects of salinity and contaminants in effluent samples collected from irrigation drain waters. Static acute effluent tests were conducted with water collected from four sites at SWMA. Animals acclimated or cultured in fresh water (fathead minnows, Pimephales promelas ; amphipods, Hyalella azteca ; cladocerans, Daphnia magna ) and salt water (striped bass, Morone saxatilis ; amphipods, Hyalella azteca ; and cladocerans, Daphnia magna ) were used to separate toxic effects of salinity from the effects of inorganic contaminants in the drain water. One drain water (TJ drain, salinity 19 parts per thousand (grams per liter), osmolality 503 mmol/kg, hardness 3,780 mg/L as CaCO 3 ) was toxic only to freshwater animals and saltwater cultured daphnids; water from a receiving pond (Pintail Bay, salinity 23 g/L, osmolality 542 mmol/kg, hardness 830 mg/L as CaCO 3 ) was toxic to both freshwater and saltwater animals. Acute tests conducted with reconstituted waters representative of the Pintail Bay sample indicated that atypical ion ratios were toxic to striped bass and amphipods, even without the addition of inorganic contaminants. However, the addition of inorganic contaminants representative of the Pintail Bay sample increased the toxicity of this reconstituted water. These findings indicate that the toxicity of the TJ drain sample was related mainly to elevated salinity and that the toxicity of the Pintail Bay sample was a function of inorganic contamination and atypical ion ratios in combination with elevated salinity.

Environmental Toxicology and Chemistry↗

Toxicity of trace element and salinity mixtures to striped bass ( Morone saxatilis ) and Daphnia magna

Acute toxicity tests with reconstituted water were conducted to investigate the relationship between water hardness, salinity, and a mixture of trace elements found in irrigation drain waters entering Stillwater Wildlife Management Area (SWMA), near Fallon, Nevada. The SWMA has been the site of many fish kills in recent years, and previous toxicity studies indicated that one drain water, Pintail Bay, was acutely toxic to organisms acclimated or cultured in fresh water or salt water. This toxicity could reflect both the ionic composition of this saline water and the presence of trace elements. The lowest water salinity tested with Daphnia magna was near the upper salinity tolerance of these organisms; therefore, we were unable to differentiate between the toxic effects of ion composition and those of trace elements. In toxicity tests conducted with striped bass ( Morone saxatilis ), we found that the extent to which salinity was lethal to striped bass depended on the ion composition of that salinity. Survival of striped bass increased as hardness increased. In addition, a trace element mixture was toxic to striped bass, even though the concentrations of individual elements were below expected acutely lethal concentrations. Although salinity is an important water quality characteristic, the ionic composition of the water must be considered when one assesses the hazard of irrigation drain waters to aquatic organisms.

Nevada↗

Role of physical barriers in the control of sea lamprey ( Petromyzon marinus )

Mechanical and electromechanical barriers played a significant role in the initial attempts to control sea lamprey ( Petromyzon marinus ) populations in the upper Great Lakes. More recently electromechanical weirs have been used to assess the relative abundance of spawning-run sea lampreys in Lake Superior. Development of an integrated control approach to sea lamprey control has stimulated an ongoing research program to define structural and/or velocity criteria that can be used to design barrier dams that block spawning runs of sea lamprey

Canadian Journal of Fisheries and Aquatic Sciences↗

Excretion of the lampricide Bayer 73 by rainbow trout

Urinary excretion of the 2-aminoethanol salt of 2′, 5-dichloro-4′-nitrosalicylanilide (Bayer 73) in rainbow trout ( Salmo gairdneri ) was measured after exposure of the trout to Bayer 73 and also after intraperitoneal (ip) injection of the lampricide. Fish exposed to 0.05 mg/litre of Bayer 73 for 12 h quickly began to excrete residues in the urine. The largest amount of Bayer 73 was excreted during the 12-h exposure, but the trout continued to excrete Bayer 73 beyond 60 h after exposure. After rainbow trout were given ip injections of 200 µ g of Bayer 73 in corn oil, they excreted up to 25 percent of the injected dose in the urine, and 20 percent was recovered in the bile at the end of the study. In both groups of fish most of the renal excretion of Bayer 73 was as the glucuronide conjugate. The exposure of rainbow trout to Bayer 73 at the doses studied had no effect on the urine output or on the renal excretion of sodium (Na + ), potassium (K + ), calcium (Ca 2+ ), magnesium (Mg 2+ ), and chloride (Cl - ).

Conference Paper↗

Chemical composition of blood and bile of the shovelnose sturgeon

Samples of gallbladder bile and blood from shovelnose sturgeons (Scaphirhynchus platorynchus) collected from the Chippewa River, Wisconsin, contained concentrations of Na + , K + , Ca ++ , Mg ++ , Cl - , inorganic phosphate, and total cholesterol closely comparable with those reported for similar samples from other species of freshwater sturgeons.

Progressive Fish-Culturist↗

Residue dynamics of quinaldine and TFM in rainbow trout

Study of the residue dynamics of 2-methylquinoline (quinaldine) and 3-trifluoromethyl-4-nitrophenol (TFM) in rainbow trout yielded the following findings: 1. Uptake and distribution of TFM by trout was influenced by the biotransformation of the lipidsoluble free phenol. No such effect was observed with quinaldine. 2. Disappearance of quinaldine and TFM from gallbladder bile was slower than from plasma or muscle during 24 hr of withdrawal in fresh water. 3. The concentration of TFM conjugate may exceed that of free TFM in bile by a factor of 10 3 .

General Pharmacology↗

Renal excretion in coho salmon (Oncorhynchus kisutch) after acute exposure to 3-trifluoromethyl-4-nitrophenol

Coho salmon ( Oncorhynchus kisutch ) exposed to an acute, sublethal concentration of 3-trifluoromethyl-4-nitrophenol (TFM) exhibited an increased output of urine when compared with controls, but the urinary excretion of Na, K, Ca, Mg, and Cl was not affected. About 35 times more conjugated TFM than free TFM was excreted during the 24-h study period.

Journal of the Fisheries Research Board of Canada↗