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Research about Canada, United States

Source-linked reports with geographic coverage including Canada, United States.

At least 505 records · Page 28Linked to original sources

Western bumble bee: Declines in United States and range-wide information gaps

In recent decades, many bumble bee species have declined due to changes in habitat, climate, and pressures from pathogens, pesticides, and introduced species. The western bumble bee ( Bombus occidentalis ), once common throughout western North America, is a species of concern and will be considered for listing by the U.S. Fish and Wildlife Service (USFWS) under the Endangered Species Act (ESA). We attempt to improve alignment of data collection and research with USFWS needs to consider redundancy, resiliency, and representation in the upcoming species status assessment. We reviewed existing data and literature on B. occidentalis , highlighting information gaps and priority topics for research. Priorities include increased knowledge of trends, basic information on several life‐history stages, and improved understanding of the relative and interacting effects of stressors on population trends, especially the effects of pathogens, pesticides, climate change, and habitat loss. An understanding of how and where geographic range extent has changed for the two subspecies of B. occidentalis is also needed. We outline data that could be easily collected in other research projects that would increase their utility for understanding range‐wide trends of bumble bees. We modeled the overall trend in occupancy from 1998 to 2018 of Bombus occidentalis within the continental United States using existing data. The probability of local occupancy declined by 93% over 21 yr from 0.81 (95% CRI = 0.43, 0.98) in 1998 to 0.06 (95% CRI = 0.02, 0.16) in 2018. The decline in occupancy varied spatially by landcover and other environmental factors. Detection rates vary in both space and time, but peak detection across the continental United States occurs in mid‐July. We found considerable spatial gaps in recent sampling, with limited sampling in many regions, including most of Alaska, northwestern Canada, and the southwestern United States. We therefore propose a sampling design to address these gaps to best inform the ESA species status assessment through improved assessment of how the spatial distribution of stressors influences occupancy changes. Finally, we request involvement via data sharing, participation in occupancy sampling with repeated visits to distributed survey sites, and complementary research to address priorities outlined in this paper.

Ecosphere↗

A review of pathogens, diseases, and contaminants of muskrats (Ondatra zibethicus) in North America

Over the last 50 years, significant muskrat (Ondatra zibethicus) harvest declines have been observed throughout North America. Several theories for the decline have been proposed, including increased parasite infections and disease within muskrat populations. No existing wholistic review of muskrat exposure to pathogens, contaminants, and diseases exists. To address this knowledge gap, we conducted a thorough review of existing literature on muskrat pathogens, contaminants, and diseases across their natural range. This review is comprised of 129 articles from 1915-2019and from 27 U.S. states and 9 Canadian provinces. A wide diversity of contaminants, toxins, and pathogens were reported in muskrats, with the most common diseases being cysticercosis, tularemia, Tyzzer’s disease, and biotoxin poisoning from cyanobacteria. This review provides a summary of muskrat pathogens, contaminants, and disease over a century that has observed significant population declines throughout the species’ range in North America. Such data provides baseline data for understanding the potential role of disease in these declines. In addition, these data highlight critical knowledge gaps that warrant future research efforts.

Alaska, Alberta, British Columbia, Colorado, Idaho↗

Projected impacts of climate change on the range and phenology of three culturally-important shrub species

Climate change is shifting both the habitat suitability and the timing of critical biological events, such as flowering and fruiting, for plant species across the globe. Here, we ask how both the distribution and phenology of three food-producing shrubs native to northwestern North America might shift as the climate changes. To address this question, we compared gridded climate data with species location data to identify climate variables that best predicted the current bioclimatic niches of beaked hazelnut ( Corylus cornuta) , Oregon grape ( Mahonia aquifolium ), and salal ( Gaultheria shallon ). We also developed thermal-sum models for the timing of flowering and fruit ripening for these species. We then used multi-model ensemble future climate projections to estimate how species range and phenology may change under future conditions. Modelling efforts showed extreme minimum temperature, climate moisture deficit, and mean summer precipitation were predictive of climatic suitability across all three species. Future bioclimatic niche models project substantial reductions in habitat suitability across the lower elevation and southern portions of the species’ current ranges by the end of the 21 st century. Thermal-sum phenology models for these species indicate that flowering and the ripening of fruits and nuts will advance an average of 25 days by the mid-21 st century, and 36 days by the late-21 st century under a high emissions scenario (RCP 8.5). Future changes in the climatic niche and phenology of these important food-producing species may alter trophic relationships, with cascading impacts on regional ecosystems.

British, Columbia, California, Idaho, Montana, Nev↗

Earthquake early warning ShakeAlert 2.0: Public rollout

The ShakeAlert Earthquake Early Warning System is designed to automatically identify and characterize the initiation and rupture evolution of large earthquakes, estimate the intensity of ground shaking that will result, and deliver alerts to people and systems that may experience shaking, prior to the occurrence of shaking at their location. It is configured to issue alerts to locations within the West Coast of the U.S. In 2018, ShakeAlert 2.0 went live in a regional public test in the first phase of a general public rollout. The ShakeAlert system is now providing alerts to over sixty institutional partners in the three states of the Western U.S. where most of the nation’s earthquake risk is concentrated: California, Oregon, and Washington. The ShakeAlert 2.0 product for public alerting is a message containing a polygon enclosing a region predicted to experience Modified Mercalli Intensity ≥ IV for an earthquake of M5.0 or larger, corresponding to moderate-to-strong ground shaking. A polygon format alert is the easiest description for selective re-broadcasting mechanisms (e.g. cell towers) and is a requirement for some mass notification systems such as the Federal Emergency Management Agency’s Integrated Public Alert and Warning System. ShakeAlert 2.0 is tested using historic waveform data consisting of 60 M3.5+ and 25 M5.0+ earthquakes, in addition to other anomalous waveforms. For the historic event test, the average M5+ false alert rate/missed event rate for ShakeAlert 2.0 is 8%/16%, and the M3.5+ false alert rate/missed event rate is 10%/36.7%. Real-time performance metrics are also presented to assess how the system behaves in regions that are well-instrumented, sparsely instrumented, and offshore.

British Columbia, California, Oregon, Washinton↗

Egg and larval collection methods affect spawning adult numbers inferred by pedigree analysis

Analytical methods that incorporate genetic data are increasingly used in monitoring and assessment programs for important rate functions of fish populations (e.g., recruitment). Because gear types vary in efficiencies and effective sampling areas, results from genetic‐based assessments likely differ depending on the sampling gear used to collect genotyped individuals; consequently, management decisions may also be affected by sampling gear. In this study, genetic pedigree analysis conducted on egg and larval Lake Sturgeon Acipenser fulvescens collected from the St. Clair–Detroit River system using three gear types was used to estimate and evaluate gear‐specific differences in the number of spawning adults that produced the eggs and larvae sampled ( N s ), the effective number of breeding adults ( N b ), and individual reproductive success. Combined across locations and sampling years, pooled estimates were 330 ( N s ; point estimate) and 317 ( N b ; 95% CI = 271–372). Mean reproductive success was 4.35 with a variance of 5.33 individuals/spawner. Mean ± SE estimated numbers of unique parents per genotyped egg or larva (i.e., adult detection rate) from 2015 samples were 1.140 ± 0.003 for vertically stratified conical nets, 0.836 ± 0.002 for D‐frame nets, and 0.870 ± 0.002 for egg mats. Using samples from 2016, adult detection rates were 0.823 ± 0.001 for D‐frame nets and 0.708 ± 0.001 for egg mat collections. Coancestry values were negatively correlated with adult detection rate. Although genetic pedigree analyses can improve the understanding of recruitment in fish populations, this study demonstrates that estimates from genetic analyses can vary with the targeted life stage (a biologically informative outcome) and sampling methodology. This study also highlights the influence of sampling methods on the interpretation of genetic pedigree analysis results when multiple gear types are used to collect individuals. Development of standardization approaches may facilitate spatial and temporal comparisons of genetic‐based assessment results.

Michigan, Ontario↗

Detecting commonality in multidimensional fish movement histories using sequence analysis

Background Acoustic telemetry, for tracking fish movement histories, is multidimensional capturing both spatial and temporal domains. Oftentimes, analyses of such data are limited to a single domain, one domain nested within the other, or ad hoc approaches that simultaneously consider both domains. Sequence analysis, on the other hand, offers a repeatable statistical framework that uses a sequence alignment algorithm to calculate pairwise dissimilarities among individual movement histories and then hierarchical agglomerative clustering to identify groups of fish with similar movement histories. The objective of this paper is to explore how acoustic telemetry data can be fit to this statistical framework and used to identify commonalities in the movement histories of acoustic-tagged sea lamprey during upstream migration through the St. Clair-Detroit River System. Results Five significant clusters were identified among individual fish. Clusters represented differences in timing of movements (short vs long duration in the Detroit R. and Lake St. Clair); extent of upstream migration (ceased migration in Lake St. Clair, lower St. Clair R., or upper St. Clair R.), and occurrence of fallback (return to Lake St. Clair after ceasing migration in the St. Clair R.). Inferences about sea lamprey distribution and behavior from these results were similar to those reached in a previous analysis using ad-hoc analysis methods. Conclusions The repeatable statistical framework outlined here can be used to group sea lamprey movement histories based on shared sequence characteristics (i.e., chronological order of “states” occupied). Further, this framework is flexible and allows researchers to define a priori the movement aspect (e.g., order, timing, duration) that is important for identifying both common or previously undetected movement histories. As such, we do not view sequence analysis as a panacea but as a useful complement to other modelling approaches (i.e., exploratory tool for informing hypothesis development) or a stand-alone semi-quantitative method for generating a simplified, temporally and spatially structured view of complex acoustic telemetry data and hypothesis testing when observed patterns warrant further investigation.

Michigan↗

The geometry of reaction norms yields insights on classical fitness functions for Great Lakes salmon

Life history theory examines how characteristics of organisms, such as age and size at maturity, may vary through natural selection as evolutionary responses that optimize fitness. Here we ask how predictions of age and size at maturity differ for the three classical fitness functions–intrinsic rate of natural increase r , net reproductive rate R 0 , and reproductive value V x −for semelparous species. We show that different choices of fitness functions can lead to very different predictions of species behavior. In one’s efforts to understand an organism’s behavior and to develop effective conservation and management policies, the choice of fitness function matters. The central ingredient of our approach is the maturation reaction norm (MRN), which describes how optimal age and size at maturation vary with growth rate or mortality rate. We develop a practical geometric construction of MRNs that allows us to include different growth functions (linear growth and nonlinear von Bertalanffy growth in length) and develop two-dimensional MRNs useful for quantifying growth-mortality trade-offs. We relate our approach to Beverton-Holt life history invariants and to the Stearns-Koella categorization of MRNs. We conclude with a detailed discussion of life history parameters for Great Lakes Chinook Salmon and demonstrate that age and size at maturity are consistent with predictions using R 0 (but not r or V x ) as the underlying fitness function.

Great Lakes↗

Co-producing knowledge: The Integrated Ecosystem Model for resource management in Arctic Alaska

Assessments of climate-change effects on ecosystem processes and services in high-latitude regions are hindered by a lack of decision-support tools capable of forecasting possible future landscapes. We describe a collaborative effort to develop and apply the Integrated Ecosystem Model (IEM) for Alaska and northwestern Canada to explore how climate change influences interactions among disturbance regimes, permafrost integrity, hydrology, and vegetation, and how these dynamics in turn influence resource management decisions. This process emphasizes co-production of knowledge among decision makers, scientists, major funders, partners, and stakeholders. We highlight research findings based on IEM applications in Arctic Alaska, as well as successes and challenges of the co-production process. The overall framework and lessons from our work with the IEM are relevant to other collaborative efforts outside the Arctic that aim to develop a decision-support tool or an undertaking of equivalent scope.

Alaska↗

Geology and assessment of undiscovered oil and gas resources of the Sverdrup Basin Province, Arctic Canada, 2008

The Sverdrup Basin Province, an area of 515,000 square kilometers on the northern margin of North America, extends 1,300 kilometers across the Canadian Arctic Islands from near the Mackenzie Delta to northern Ellesmere Island. It consists of an intracratonic late Paleozoic to early Cenozoic rift-sag basin and a Mesozoic rift shoulder that bounds it on the north. Basin inception was Mississippian, manifested by deposition of nonmarine strata in rift basins, followed by Pennyslvanian marine transgression, which began with evaporites and progressed to Permian carbonate and clastic deposition at basin fringes and organic-rich marine strata in the basin center. Sediment transport was both northward from North America and southward from a now-subsided or rifted-away landmass to the north. Mesozoic strata indicate continued marine deposition, including both organic-rich, fine-grained rocks deposited during highstands and progradational deltaic sequences. A new episode of rifting began in Middle Jurassic time and culminated in the opening of the Canada Basin by Early Cretaceous seafloor spreading. The Sverdrup Rim formed as the rift shoulder between North America and the thinned, subsided crust to the north. Widespread Upper Cretaceous organic-rich shales were deposited during the major transgression induced by Canada Basin opening, followed by an influx of coarser east-derived detritus. In Paleogene time, incipient North Atlantic seafloor spreading caused deformation in northeasternmost North America, producing uplifts that shed detritus westward across the Sverdrup Basin. Tight folding and thrusting resulting from the Eurekan orogeny took place in the eastern part of the basin during the Eocene, with decreasing intensity of deformation westward. Since deformation ended in late Eocene time, little significant tectonism or deposition has taken place. Two petroleum systems were defined in the Sverdrup Basin Province. Upper Paleozoic marine shale generated petroleum beginning in the Early Triassic, but this petroleum system was not quantitatively assessed because reservoir quality in adjacent strata is poor, the rocks are mostly overmature, and subsequent deformation likely affected trap integrity. The second petroleum system was sourced by Lower Triassic strata rich in oil-prone organic matter. Oil was generated during Paleogene burial synchronous with Eurekan deformation, and the oil migrated into Triassic and Jurassic deltaic, shallow marine and nonmarine strata. However, most of the oil may have escaped during deformation and subsequent uplift and erosion, which probably caused oil to be displaced from traps by gas expansion. The population of undiscovered accumulations was characterized as likely to include stratigraphically trapped and small, structurally trapped accumulations, with a median size of 80 million barrels of oil (MMBO); the number of undiscovered accumulations was estimated to be between 1 and 50, with the most likely number being 10. The resulting estimate of undiscovered, technically recoverable, conventional oil resources is 61 to 1,255 MMBO, with a mean of 427 MMBO. Undiscovered, technically recoverable, conventional gas resources are estimated at 4.95 trillion cubic feet (TCF), with slightly more than half of that in nonassociated gas accumulations. A third petroleum system in the adjacent Amerasia Basin Province to the north was considered somewhat likely to contain accumulations on the Sverdrup Rim. Deeply buried Upper Jurassic, Upper Cretaceous, and Eocene organic-rich strata probably generated oil that may have migrated up the continental slope into Triassic to Paleogene sandstones on the Sverdrup Rim. Based on analogy with the Barrow Arch in Alaska, a median of 20 accumulations was estimated, with accumulation volumes as much as 2,500 MMBO and a median of 100 MMBO. The probability of at least one accumulation of the minimum size assessed (50 MMBO) was estimated at 0.22. The resulting estimate of undiscovered, technically recoverable, conventional oil resources is 0 to 2,679 MMBO, with a mean of 424 MMBO. Mean estimates for associated and nonassociated gas are 1.3 and 2.3 TCF, respectively.

Sverdrup Basin↗

Earthquake magnitude and Lg Q variations between the Grenville and northern Appalachian geologic provinces of eastern Canada

This article assesses the ability of regionally specific, frequency‐dependent crustal attenuation ( ⁠ 1 / Q "> 1 / Q ⁠ ) to reduce mean magnitude discrepancies between seismic stations in the northern Appalachian and Grenville provinces (NAP and GP) of Canada. Lg Q ( f ) "> Q ( f ) is an important parameter in ground‐motion models used in probabilistic seismic hazard analysis. Discrepancies in regional magnitude estimates have long been noted to exist between stations in the two provinces for common event origins. Such discrepancies could arise from systematic site condition variations between the geologic provinces or from varying crustal attenuative properties. To evaluate the effect of frequency‐dependent anelastic attenuation, Lg Q ( f ) "> Q ( f ) on estimated magnitudes, we analyze Lg amplitudes from > 6000 "> > 6000 waveforms recorded by Grenville and northern Appalachian receivers from 420 natural earthquakes of M N "> M N magnitude 3–5.6. Waveform analysis is strictly limited to analyst‐reviewed, vertical‐component waveforms in which Lg is clearly identified, ensuring that the datasets exhibit dominant, high‐frequency energy in the Lg velocity window. Lg Q ( f ) "> Q ( f ) is found to be higher in the GP than in the northern Appalachians. In the Grenville, Q ( f ) = 761 ( ± 145 ) f 0.25 ( ± 0.014 ) "> Q ( f ) = 761 ( ± 145 ) f 0.25 ( ± 0.014 ) ⁠ , and in the northern Appalachians, attenuation is higher: Q ( f ) = 506 ( ± 172 ) f 0.33 ( ± 0.310 ) "> Q ( f ) = 506 ( ± 172 ) f 0.33 ( ± 0.310 ) . Earthquake magnitude determined using the peak amplitude of the Lg phase ( ⁠ m b L g "> m b L g ) for eastern Canada is corrected to incorporate the frequency‐dependent, regionally specific Lg Q ( f ) "> Q ( f ) determined in this study. Using the new Lg Q ( f ) "> Q ( f ) values diminishes and nearly resolves magnitude discrepancies between the provinces. Correcting regional magnitude discrepancies between provinces is critical for reliable regional seismic hazard estimates because magnitude error in a particular region could lead to increased uncertainty in seismic hazard models.

Grenville Geologic Province, Northern Appalachian ↗

RAD-seq refines previous estimates of genetic structure in Lake Erie walleye

Delineating population structure helps fishery managers to maintain a diverse “portfolio” of local spawning populations (stocks), as well as facilitate stock-specific management. In Lake Erie, commercial and recreational fisheries for Walleye Sander vitreus exploit numerous local spawning populations, which cannot be easily differentiated using traditional genetic data (e.g., microsatellites). Here, we used genomic information (12,264 polymorphic loci) generated using restriction site-associated DNA sequencing to investigate stock structure in Lake Erie Walleye. We found low genetic divergence (genetic differentiation index F ST = 0.0006–0.0019) among the four Lake Erie western basin stocks examined, which resulted in low classification accuracies for individual samples (40–60%). However, more structure existed between western and eastern Lake Erie basin stocks ( F ST = 0.0042–0.0064), resulting in greater than 95% classification accuracy of samples to a lake basin. Thus, our success in using genomics to identify stock structure varied with spatial scale. Based on our results, we offer suggestions to improve the efficacy of this new genetic tool for refining stock structure and eventually determining relative stock contributions in Lake Erie Walleye and other Great Lakes populations.

Michigan, New York, Ohio, Ontario, Pennsylvania↗

How "simple" methodological decisions affect interpretation of population structure based on reduced representation library DNA sequencing: A case study using the lake whitefish

Reduced representation (RRL) sequencing approaches (e.g., RADSeq, genotyping by sequencing) require decisions about how much to invest in genome coverage and sequencing depth, as well as choices of values for adjustable bioinformatics parameters. To empirically explore the importance of these “simple” methodological decisions, we generated two independent sequencing libraries for the same 142 individual lake whitefish (Coregonus clupeaformis) using a nextRAD RRL approach: (1) a larger number of loci at low sequencing depth based on a 9mer (library A); and (2) fewer loci at higher sequencing depth based on a 10mer (library B). The fish were selected from populations with different levels of expected genetic subdivision. Each library was analyzed using the STACKS pipeline followed by three types of population structure assessment (FST, DAPC and ADMIXTURE) with iterative increases in the stringency of sequencing depth and missing data requirements, as well as more specific a priori population maps. Library B was always able to resolve strong population differentiation in all three types of assessment regardless of the selected parameters, largely due to retention of more loci in analyses. In contrast, library A produced more variable results; increasing the minimum sequencing depth threshold (-m) resulted in a reduced number of retained loci, and therefore lost resolution at high -m values for FST and ADMIXTURE, but not DAPC. When detecting fine population differentiation, the population map influenced the number of loci and missing data, which generated artefacts in all downstream analyses tested. Similarly, when examining fine scale population subdivision, library B was robust to changing parameters but library A lost resolution depending on the parameter set. We used library B to examine actual subdivision in our study populations. All three types of analysis found complete subdivision among populations in Lake Huron, ON and Dore Lake, SK, Canada using 10,640 SNP loci. Weak population subdivision was detected in Lake Huron with fish from sites in the north-west, Search Bay, North Point and Hammond Bay,showing slight differentiation. Overall, we show that apparently simple decisions about library construction and bioinformatics parameters can have important impacts on the interpretation of population subdivision. Although potentially more costly on a per-locus basis, early investment in striking a balance between the number of loci and sequencing effort is well worth the reduced genomic coverage for population genetics studies. More conservative stringency settings on STACKS parameters lead to a final dataset that was more consistent and robust when examining both weak and strong population differentiation. Overall, we recommend that researchers approach “simple” methodological decisions with caution, especially when working on non-model species for the first time.

Michigan, Ontario, Saskatchewan↗

Revision of Boore (2018) Ground‐motion predictions for Central and Eastern North America: Path and offset adjustments and extension to 200 m/s <= Vs30 <= 3000 m/s

The three sets of ground‐motion predictions (GMPs) of Boore (2018 ; hereafter, B18) are compared with a much larger dataset than was used in deriving the predictions. The B18 GMPs work well for response spectra at periods between &#x223C; 0.15 "> ∼ 0.15 ∼0.15 and 4.0 s after an adjustment accounting for a path bias at distances beyond 200 km—this was the maximum distance used to derive the stress parameters on which the simulations in B18 are based. An additional offset adjustment is needed in the B18 predictions for short and long periods. The adjustment at short periods may be because the &#x3BA; 0 "> κ 0 κ0 of 0.006 s stipulated by the Next Generation Attenuation‐East (NGA‐East) project to be used in deriving the GMPs is inconsistent with the observations on rock sites. The explanation for the offset adjustment at long periods is not clear, but it could be a combination of limitations of the point‐source stochastic model for longer period motions, as well as a decreasing number of observations at longer periods available to constrain the simulations on which the predictions are based. The predictions of B18, developed for very‐hard‐rock sites ( ⁠ V S 30 "> V S 30 VS30 of 2000 and 3000 &#x2009;&#x2009; m / s "> 3000 m / s 3000 m/s ⁠ ), have here been extended down to V S 30 "> V S 30 VS30 values as low as 200 &#x2009;&#x2009; m / s "> 200 m / s 200 m/s ⁠ . I find, as have others, that for a given V S 30 "> V S 30 VS30 ⁠ , there is generally less site amplification for central and eastern North America (CENA) than for the active crustal region dataset used for the Boore, Stewart, et al. (2014 ; hereafter, BSSA14) GMP equations. This might have an impact on conclusions of several previous studies of CENA GMPs that used the site amplifications in BSSA14 in comparing data and predictions. An additional finding is that the &#x3BA; 0 "> κ 0 κ0 implied by recordings on a subset of stations in the Charlevoix region located on rock (data from these stations were not used in the analysis described earlier) is more consistent with a value near 0.014 s than the 0.006 s value used in B18 and the NGA‐East project.

Seismological Research Letters↗

RAPTURE (RAD capture) panel facilitates analyses characterizing sea lamprey reproductive ecology and movement dynamics

Genomic tools are lacking for invasive and native populations of sea lamprey ( Petromyzon marinus ). Our objective was to discover single nucleotide polymorphism (SNP) loci to conduct pedigree analyses to quantify reproductive contributions of adult sea lampreys and dispersion of sibling larval sea lampreys of different ages in Great Lakes tributaries. Additional applications of data were explored using additional geographically expansive samples. We used restriction site‐associated DNA sequencing (RAD‐Seq) to discover genetic variation in Duffins Creek (DC), Ontario, Canada, and the St. Clair River (SCR), Michigan, USA. We subsequently developed RAD capture baits to genotype 3,446 RAD loci that contained 11,970 SNPs. Based on RAD capture assays, estimates of variance in SNP allele frequency among five Great Lakes tributary populations (mean F ST 0.008; range 0.00–0.018) were concordant with previous microsatellite‐based studies; however, outlier loci were identified that contributed substantially to spatial population genetic structure. At finer scales within streams, simulations indicated that accuracy in genetic pedigree reconstruction was high when 200 or 500 independent loci were used, even in situations of high spawner abundance (e.g., 1,000 adults). Based on empirical collections of larval sea lamprey genotypes, we found that age‐1 and age‐2 families of full and half‐siblings were widely but nonrandomly distributed within stream reaches sampled. Using the genomic scale set of SNP loci developed in this study, biologists can rapidly genotype sea lamprey in non‐native and native ranges to investigate questions pertaining to population structuring and reproductive ecology at previously unattainable scales.

Michigan, Ontario, Wisconsin↗

Climate-change refugia in boreal North America: What, where, and for how long?

T The vast boreal biome plays an important role in the global carbon cycle but is experiencing particularly rapid climate warming, threatening the integrity of valued ecosystems and their component species. We developed a framework and taxonomy to identify climate‐change refugia potential in the North American boreal region, summarizing current knowledge regarding mechanisms, geographic distribution, and landscape indicators. While “terrain‐mediated” refugia will mostly be limited to coastal and mountain regions, the ecological inertia (resistance to external fluctuations) contained in some boreal ecosystems may provide more extensive buffering against climate change, resulting in “ecosystem‐protected” refugia. A notable example is boreal peatlands, which can retain high surface soil moisture and water tables even in the face of drought. Refugia from wildfire are also especially important in the boreal region, which is characterized by active disturbance regimes. Our framework will help identify areas of high refugia potential, and inform ecosystem management and conservation planning in light of climate change.

North America↗

If you build it and they come, will they stay? Maturation of constructed fish spawning reefs in the St. Clair-Detroit River System

Constructed rock reefs have been used to remediate spawning habitat for Lake Sturgeon ( Acipenser fulvescens ) and other lithophilic spawning fishes in the St. Clair-Detroit River System, North America. Early projects used a cross-channel design and species-specific metrics (e.g., proximity to historical spawning locations) to guide reef placement. However, the Middle Channel Reefs and portions of other early projects were compromised by fine sediment accumulation. Therefore, geomorphological criteria were considered in siting reefs constructed after 2013 to avoid sediment sources and improve the likelihood of successful reef function. To evaluate the effectiveness of the revised placement process, we quantified physical maturation of constructed reefs using annual side-scan and down-looking sonar surveys beginning in 2014 and underwater video surveys beginning in 2015. Reef areas and hardness were measured from sonar surveys and underwater video was used to quantify surficial sediment composition. Size and hardness of reefs developed using geomorphological criteria decreased with time, but at rates slower than what was observed at the Middle Channel Reefs. Sediment composition of the reefs remained similar through 2017 and prevalence of reef rock was high, except at Hart's Light Reef, where dreissenid mussel shells composed 32% of the surficial substrate by age three. However, accumulation of fine sediments was documented at all reefs in 2018. Despite using geomorphic criteria to identify areas most suitable for reef construction, reef sediment composition has changed, and future reef restoration projects could benefit by incorporating methods for maintenance, in addition to using geomorphic criteria, to identify restoration sites.

Michigan, Ontario↗

Differentiation between lake whitefish and cisco eggs based on diameter

Cisco ( Coregonus artedi ) and lake whitefish ( Coregonus clupeaformis ) are native fish species of management concern in the Laurentian Great Lakes that often overlap in spawning locations and timing. Thus, species-level inference from in situ sampling requires methods to differentiate their eggs. Genetic barcoding and hatching eggs to visually identify larvae are used but can be time and cost intensive. Observations in published literature indicate that lake whitefish eggs may be larger than cisco eggs in the Great Lakes, but this has not yet been substantiated. Samples from shared spawning grounds are unlikely to contain similarly sized or colored eggs from other species. Thus, we assessed whether lake whitefish and cisco eggs could be separated based on size alone. Fertilized, hardened eggs were collected in situ during spawning at Elk Rapids, Lake Michigan and Chaumont Bay, Lake Ontario and preserved in ethanol. Individual eggs were measured and genetically identified. Mean diameter for cisco (2.45 mm, SD = 0.22, n = 444) was smaller than for lake whitefish (3.21 mm, SD = 0.20, n = 99). We used classification trees to identify a species-separating size threshold of 2.88 mm (95% bootstrap CI = [2.877, 2.976]), which classified eggs with an accuracy rate of 96%. Differences between species across other samples from the same locations were mostly consistent with the threshold size, but we suggest validation if applying this method to other populations. Separation of cisco and lake whitefish eggs by diameter can be accurate, efficient, and especially suitable for large sample sizes.

Lake Michigan, Lake Ontario↗

Gut contents from multiple morphs of lake trout (Salvelinus namaycush) at two offshore shoals in Lake Superior

Four lake trout, Salvelinus namaycush , Walbaum 1792 morphs occur in Lake Superior: lean, siscowet, humper, and redfin. Diets of lean and siscowet have been relatively well described. However, less is known about diets of humper and redfin, and overall few studies have been conducted at offshore shoals. We compared gut content data among mature (357–867 mm) sympatric lake trout morphs caught at two offshore shoals in Lake Superior, Stannard Rock and Superior Shoal, in 2013 and 2014 (total n = 416). All morphs were caught in shallow (<50 m), mid (50–100 m), and deep (>100 m) strata. Invertebrates made up a greater portion of the stomach contents than did fish for all morphs by both percent occurrence and proportional biomass, and Mysis was the primary invertebrate consumed by all morphs at both sites. Coregonus spp. and deepwater sculpin, Myoxocephalus thompsonii were the most commonly consumed fish. Humper had the highest average proportional biomass of deepwater sculpin and had no other identifiable species of fish in their guts. Biomass of fish in redfin guts was highest for Coregonus spp., followed by similar amounts of deepwater sculpin and burbot, Lota lota . Diet overlap among morphs was high, and differences in prey consumption between sites are likely related to prey availability. Additional study is needed to determine if differences in trophic ecology between humper and other morphs are sufficient to support concurrent stocking of multiple morphs, particularly in light of recent declines in native prey fishes, especially Coregonus spp., in the Laurentian Great Lakes.

Lake Superior↗