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Pilot study for U.S. Geological Survey Standard Reference Water Samples for pesticides

The U.S. Geological Survey has been preparing and maintaining a library of standard reference water samples for inorganic constituents for 19 years. Recently, a pilot study was conducted to see if the reference-sample program could be expanded to include pesticides and other organic materials. Two samples containing organochlorine and organophosphorus insecticides, and chlorophenoxy acid herbicides were distributed to a number of laboratories in the United States. One of the samples also contained polychlorinated biphenyls. Interlaboratory data obtained from these round robin studies are presented with intralaboratory information on long-term stability.

Journal of Testing and Evaluation

A standard-reference water-suspended sediment sample for total recoverable metals

The U.S. Geological Survey has been preparing and maintaining a library of standard-reference water samples for dissolved inorganic constituents for 19 years. Recently, the reference-sample program was expanded to include a water-suspended sediment mixture for the determination of total recoverable metals. An interlaboratory round-robin study was conducted. Digestion procedures used by the U.S. Geological Survey and the U.S. Environmental Protection Agency were used to solubilize the metals before their measurement. The data indicate the both digestion procedures for total recoverable metals are essentially equivalent. Precision data are comparable to those data obtained in standard-reference water samples for dissolved metals.

Journal of Testing and Evaluation

Assessing effects of pesticides on amphibians and reptiles: status and needs

Growing concern about the decline of certain amphibian populations and for conservation of amphibians and reptiles has led to renewed awareness of problems from pesticides. Testing amphibians and reptiles as a requirement for chemical registration has been proposed but is difficult because of the phylogenetic diversity of these groups. Information from the literature and research may determine whether amphibians and reptiles are adequately protected by current tests for mammals, birds, and fish. Existing information indicates that amphibians are unpredictably more resistant to certain cholinesterase inhibitors, and more sensitive to two chemicals used in fishery applications than could have been predicted. A single study on one species of lizard suggests that reptiles may be close in sensitivity to mammals and birds. Research on effects of pesticides on amphibians and reptiles should compare responses to currently tested groups and should seek to delineate those taxa and chemicals for which cross-group prediction is not possible. New tests for amphibians and reptiles should rely to the greatest extent possible on existing data bases, and should be designed for maximum economy and minimum harm to test animals. A strategy for developing the needed information is proposed. Good field testing and surveillance of chemicals in use may compensate for failures of predictive evaluations and may ultimately lead to improved tests.

Herpetological Journal

Increased scientific rigor will improve reliability of research and effectiveness of management

Rigorous science that produces reliable knowledge is critical to wildlife management because it increases accurate understanding of the natural world and informs management decisions effectively. Application of a rigorous scientific method based on hypothesis testing minimizes unreliable knowledge produced by research. To evaluate the prevalence of scientific rigor in wildlife research, we examined 24 issues of the Journal of Wildlife Management from August 2013 through July 2016. We found 43.9% of studies did not state or imply a priori hypotheses, which are necessary to produce reliable knowledge. We posit that this is due, at least in part, to a lack of common understanding of what rigorous science entails, how it produces more reliable knowledge than other forms of interpreting observations, and how research should be designed to maximize inferential strength and usefulness of application. Current primary literature does not provide succinct explanations of the logic behind a rigorous scientific method or readily applicable guidance for employing it, particularly in wildlife biology; we therefore synthesized an overview of the history, philosophy, and logic that define scientific rigor for biological studies. A rigorous scientific method includes 1) generating a research question from theory and prior observations, 2) developing hypotheses (i.e., plausible biological answers to the question), 3) formulating predictions (i.e., facts that must be true if the hypothesis is true), 4) designing and implementing research to collect data potentially consistent with predictions, 5) evaluating whether predictions are consistent with collected data, and 6) drawing inferences based on the evaluation. Explicitly testing a priori hypotheses reduces overall uncertainty by reducing the number of plausible biological explanations to only those that are logically well supported. Such research also draws inferences that are robust to idiosyncratic observations and unavoidable human biases. Offering only post hoc interpretations of statistical patterns (i.e., a posteriori hypotheses) adds to uncertainty because it increases the number of plausible biological explanations without determining which have the greatest support. Further, post hoc interpretations are strongly subject to human biases. Testing hypotheses maximizes the credibility of research findings, makes the strongest contributions to theory and management, and improves reproducibility of research. Management decisions based on rigorous research are most likely to result in effective conservation of wildlife resources.

Journal of Wildlife Management

Guidelines for the field evaluation of desert tortoise health and disease

Field evaluation of free-ranging wildlife requires the systematic documentation of a variety of environmental conditions and individual parameters of health and disease, particularly in the case of rare or endangered species. In addition, defined criteria are needed for the humane salvage of ill or dying animals. The purpose of this paper is to describe, in detail, the preparation, procedures, and protocols we developed and tested for the field evaluation of wild desert tortoises ( Gopherus agassizii ). These guidelines describe: preparations for the field, including developing familiarity with tortoise behavior and ecology, and preparation of standardized data sheets; journal notes to document background data on weather conditions, temperature, rainfall, locality, and historic and recent human activities; procedures to prevent the spread of disease and parasites; data sheets for live tortoises to record tortoise identification, location, sex, body measurements and activity; health profile forms for documenting and grading physical abnormalities of tortoise posture and movements, general condition (e.g., lethargy, cachexia), external parasites, and clinical abnormalities associated with shell and upper respiratory diseases; permanent photographic records for the retrospective analysis of progression and regression of upper respiratory and eye diseases, analysis of shell lesions and evaluation of growth and age; and indications and methods for salvaging ill or dying tortoises for necropsy evaluation. These guidelines, tested on 5,000 to 20,000 tortoises over a 10 to 27 yr period, were designed to maximize acquisition of data for demographic, ecological, health and disease research projects; to reduce handling and stress of individual animals; to avoid spread of infectious disease; to promote high quality and consistent data sets; and to reduce the duration and number of field trips. The field methods are adapted for desert tortoise life cycle, behavior, anatomy, physiology, and pertinent disease; however the model is applicable to other species of reptiles. Comprehensive databases of clinical signs of disease and health are crucial to research endeavors and essential to decisions on captive release, epidemiology of disease, translocation of wild tortoises, breeding programs, and euthanasia.

Journal of Wildlife Diseases

Taking a systems approach to ecological systems

Increasingly, there is interest in a systems-level understanding of ecological problems, which requires the evaluation of more complex, causal hypotheses. In this issue of the Journal of Vegetation Science, Soliveres et al. use structural equation modeling to test a causal network hypothesis about how tree canopies affect understorey communities. Historical analysis suggests structural equation modeling has been under-utilized in ecology.

Journal of Vegetation Science

Practical application of time-lapse camera imagery to develop water-level data for three hydrologic monitoring sites in Wisconsin during water year 2020

Using camera imagery to measure water level (camera-stage) is a well-researched area of study. Previous camera-stage studies have shown promising results when implementing this technology with tight constraints on test conditions. However, there is a need for a more comprehensive evaluation of the extensibility of camera-stage to practical applications. Therefore, the aim of this study was to test a camera-stage method under a wide variety of test conditions to better understand the successes and challenges of using this technology in real-world scenarios. In this study, this approach was tested during Water Year 2020 at three existing U.S. Geological Study (USGS) stream gaging stations in south central Wisconsin that had existing USGS water-level instrumentation. The specific reference objects tested were white pipes and a concrete wall. Since successful application of camera-stage relies on use of suitable images, all captured images in this study were visually inspected to determine suitability for application of camera-stage. Camera-stage measurements were then computed only on images deemed suitable and the results were compared with ground-truth stage values to determine the accuracy. For the purposes of this study, camera-stage values within ±0.10 ft of the actual stage were considered acceptable. One major challenge highlighted was the potential difficulty in obtaining suitable imagery, with the proportion of suitable images varying greatly between the four trials from 38 % to 92 %. The results from applying camera-stage to suitable images were encouraging though, with 79 % to 99 % of evaluated camera-stage values qualifying as acceptable among the four test trials.

Wisconsin

Estimating cleanup times for groundwater contamination remediation strategies

This article presents the Natural Attenuation Software (NAS), available as a free download from Virginia Polytechnic University, which can help remedial project managers and their contractors estimate the time of groundwater remediation through consideration of such natural attenuation processes as advection, dispersion, sorption, source zone depletion, and biodegradation. NAS consists of a combination of computational tools implemented in three main interactive modules. The article discusses testing and evaluation of NAS along with expected benefits of the software tool.

Journal - American Water Works Association

A goodness-of-fit test for occupancy models with correlated within-season revisits

Occupancy modeling is important for exploring species distribution patterns and for conservation monitoring. Within this framework, explicit attention is given to species detection probabilities estimated from replicate surveys to sample units. A central assumption is that replicate surveys are independent Bernoulli trials, but this assumption becomes untenable when ecologists serially deploy remote cameras and acoustic recording devices over days and weeks to survey rare and elusive animals. Proposed solutions involve modifying the detection-level component of the model (e.g., first-order Markov covariate). Evaluating whether a model sufficiently accounts for correlation is imperative, but clear guidance for practitioners is lacking. Currently, an omnibus goodnessof- fit test using a chi-square discrepancy measure on unique detection histories is available for occupancy models (MacKenzie and Bailey, Journal of Agricultural, Biological, and Environmental Statistics, 9, 2004, 300; hereafter, MacKenzie– Bailey test). We propose a join count summary measure adapted from spatial statistics to directly assess correlation after fitting a model. We motivate our work with a dataset of multinight bat call recordings from a pilot study for the North American Bat Monitoring Program. We found in simulations that our join count test was more reliable than the MacKenzie–Bailey test for detecting inadequacy of a model that assumed independence, particularly when serial correlation was low to moderate. A model that included a Markov-structured detection-level covariate produced unbiased occupancy estimates except in the presence of strong serial correlation and a revisit design consisting only of temporal replicates. When applied to two common bat species, our approach illustrates that sophisticated models do not guarantee adequate fit to real data, underscoring the importance of model assessment. Our join count test provides a widely applicable goodness-of-fit test and specifically evaluates occupancy model lack of fit related to correlation among detections within a sample unit. Our diagnostic tool is available for practitioners that serially deploy survey equipment as a way to achieve cost savings.

Ecology and Evolution

Controlled induced recharge tests at Kalamazoo, Mich

This article discusses the results of a controlled field testing program, which indicated that definite hydraulic and other advantages may be gained from induced recharging as practiced at Kalamazoo, Michigan. Results include the following: water levels and artesian pressures can be maintained at high stages, the results are lower pumping lifts and substantial reductions in the amount of power used for pumping; the high water levels permit increased rates of withdrawal during periods of peak demand; encroachment of poor quality water from other aquifers is minimized; the surface water induced into the aquifer is filtered naturally through great thicknesses of earth materials; natural underground storage is used to conserve and protect water, which otherwise would flow largely to waste; and, significant supplemental benefits, including flood control, have been derived. The tests demonstrated that it is possible to manipulate the regimen of a complex hydrologic system for definite hydraulic benefits with predictable results. Furthermore with current methods, quantitative evaluations may be made of the effects of induced recharge. The results of the tests, therefore, are applicable in other areas of similar hydrogeologic environments.

Michigan

After site selection and before data analysis: sampling, sorting, and laboratory procedures used in stream benthic macroinvertebrate monitoring programs by USA state agencies

A survey of methods used by US state agencies for collecting and processing benthic macroinvertebrate samples from streams was conducted by questionnaire; 90 responses were received and used to describe trends in methods. The responses represented an estimated 13,000-15,000 samples collected and processed per year. Kicknet devices were used in 64.5% of the methods; other sampling devices included fixed-area samplers (Surber and Hess), artificial substrates (Hester-Dendy and rock baskets), grabs, and dipnets. Regional differences existed, e.g., the 1-m kicknet was used more often in the eastern US than in the western US. Mesh sizes varied among programs but 80.2% of the methods used a mesh size between 500 and 600 (mu or u)m. Mesh size variations within US Environmental Protection Agency regions were large, with size differences ranging from 100 to 700 (mu or u)m. Most samples collected were composites; the mean area sampled was 1.7 m 2 . Samples rarely were collected using a random method (4.7%); most samples (70.6%) were collected using "expert opinion", which may make data obtained operator-specific. Only 26.3% of the methods sorted all the organisms from a sample; the remainder subsampled in the laboratory. The most common method of subsampling was to remove 100 organisms (range = 100-550). The magnification used for sorting ranged from 1 (sorting by eye) to 30x, which results in inconsistent separation of macroinvertebrates from detritus. In addition to subsampling, 53% of the methods sorted large/rare organisms from a sample. The taxonomic level used for identifying organisms varied among taxa; Ephemeroptera, Plecoptera, and Trichoptera were generally identified to a finer taxonomic resolution (genus and species) than other taxa. Because there currently exists a large range of field and laboratory methods used by state programs, calibration among all programs to increase data comparability would be exceptionally challenging. However, because many techniques are shared among methods, limited testing could be designed to evaluate whether procedural differences affect the ability to determine levels of environmental impairment using benthic macroinvertebrate communities.

Journal of the North American Benthological Societ

When do climate services achieve societal impact? Evaluations of actionable climate adaptation science

To cope with complex environmental impacts in a changing climate, researchers are increasingly being asked to produce science that can directly support policy and decision making. To achieve such societal impact, scientists are using climate services to engage directly with stakeholders to better understand their needs and inform knowledge production. However, the wide variety of climate-services outcomes—ranging from establishing collegial relationships with stakeholders to obtaining specific information for inclusion into a pre-existing decision process—do not directly connect to traditional methods of measuring scientific impact (e.g., publication citations, journal impact factor). In this paper, we describe how concepts from the discipline of evaluation can be used to examine the societal impacts of climate services. We also present a case study from climate impacts and adaptation research to test a scalable evaluation approach. Those who conduct research for the purposes of climate services and those who fund applied climate research would benefit from evaluation from the beginning of project development. Doing so will help ensure that the approach, data collection, and data analysis are appropriately conceived and executed.

Sustainability

Rapid heat-flowing surveying of geothermal areas, utilizing individual snowfalls as calorimeters

Local differences in rate of heat transfer in vapor and by conduction through the ground in hot spring areas are difficult and time-consuming to measure quantitatively. Individual heavy snowfalls provide a rapid low-cost means of measuring total heat flow from such ground. After a favorable snowfall (heavy, brief duration, little wind, air temperature near 0°C), contacts between snow-covered and snow-free ground are mapped on a suitable base. Each mapped contact, as time elapses after a specific snowfall, is a heat-flow contour representing a decreasing rate of flow. Calibration of each mapped contact or snow line is made possible by the fact that snow remains on insulated surfaces (such as the boardwalks of Yellowstone's thermal areas) long after it has melted on adjacent warm ground. Heat-flow contours mapped to date range from 450 to 5500 μcal/cm 2 sec, or 300 to 3700 times the world average of conductive heat flow. The very high rates of heat flow (2000 to > 10,000 μcal/cm 2 sec) are probably too high, and the lower heat flows determinable by the method (<500 μcal/cm 2 sec) may be too low. Values indicated by the method are, however, probably within a factor of 2 of the total conductive and convective heat flow. Thermal anomalies from infrared imagery are similar in shape to heat-flow contours of a test area near Old Faithful geyser. Snowfall calorimetry provides a rapid means for evaluating the imagery and computer-derived products of the infrared data in terms of heat flow.

Yellowstone National Park

Coseismic and post-seismic gravity disturbance induced by seismic sources using a 2.5-D spectral element method

I present a prescription for computing free-air coseismic and post-seismic gravity changes induced by seismic sources in a viscoelastic earth model. I assume a spherical earth geometry and a 2.5-D calculation, that is, 3-D motions that satisfy the equations of quasi-static equilibrium on a 2-D viscoelastic structure. The prescription permits application to regional gravity computations where a 2-D structure adequately represents the structural heterogeneity. I use a hybrid approach where deformation is computed on a discretized domain and the resulting density perturbations are expanded with spherical harmonics to produce the free-air gravity field. Starting with a solution to the equations of quasi-static displacements in the Laplace transform domain for a given dislocation source, I solve Poisson’s equation using Lagrangian interpolation on spectral element nodes to compute the required deformation quantities that contribute to free-air gravity. A numerical inverse Laplace transform then yields time domain results. This methodology is tested with analytic solutions on a spherically stratified viscoelastic structure, then applied to evaluate the effect of a descending slab of relatively high viscosity on post-seismic gravity in a megathrust faulting setting.

Geophysical Journal International

Predicting vertically-nonsequential wetting patterns with a source-responsive model

Water infiltrating into soil of natural structure often causes wetting patterns that do not develop in an orderly sequence. Because traditional unsaturated flow models represent a water advance that proceeds sequentially, they fail to predict irregular development of water distribution. In the source-responsive model, a diffuse domain (D) represents flow within soil matrix material following traditional formulations, and a source-responsive domain (S), characterized in terms of the capacity for preferential flow and its degree of activation, represents preferential flow as it responds to changing water-source conditions. In this paper we assume water undergoing rapid source-responsive transport at any particular time is of negligibly small volume; it becomes sensible at the time and depth where domain transfer occurs. A first-order transfer term represents abstraction from the S to the D domain which renders the water sensible. In tests with lab and field data, for some cases the model shows good quantitative agreement, and in all cases it captures the characteristic patterns of wetting that proceed nonsequentially in the vertical direction. In these tests we determined the values of the essential characterizing functions by inverse modeling. These functions relate directly to observable soil characteristics, rendering them amenable to evaluation and improvement through hydropedologic development.

Vadose Zone Journal

Preliminary study of rock alteration in the Catheart Mountain molybdenum-copper deposit, Maine

Studies of drill cores from the Catheart Mountain molybdenum-copper deposit indicate that the deposit is of the porphyry type. Hydrothermal alteration and sulfide mineralization are probably distributed in complex but systematic alteration zones. Most or all mineralization at Catheart Mountain is in a medium-fine-grained quartz monzonite enclosed within a larger mass of Attean Quartz Monzonite; no ore and little hydrothermal alteration have been seen in the Attean. Much of the ore and hydrothermal alteration seem to be and presumably are spatially related to irregular intrusive masses of porphyry. Only certain porphyry intrusives are mineral bearing, however, and many have no associated ore and alteration. Studies of hydrothermal alterations in surface exposures supplemented by rock analyses of bulk samples indicate that alteration features and sulfide zone characteristics of an area northwest of the mountain crest are, when measured against accepted porphyry models, very favorable for the occurrence of ore. Even though this favorable area has already been tested by some drilling, the presence together here of the strong center of potassic enrichment, the well-developed pyrite zone, and the strong and extensive phyllic alteration suggest that some further evaluation may be warranted.

Maine

The precision and accuracy of iterative and non-iterative methods of photopeak integration in activation analysis, with particular reference to the analysis of multiplets

The relative precisions obtainable using two digital methods, and three iterative least squares fitting procedures of photopeak integration have been compared empirically using 12 replicate counts of a test sample with 14 photopeaks of varying intensity. The accuracy by which the various iterative fitting methods could analyse synthetic doublets has also been evaluated, and compared with a simple non-iterative approach. ?? 1977 Akade??miai Kiado??.

Journal of Radioanalytical Chemistry

A vectorial capacity product to monitor changing malaria transmission potential in epidemic regions of Africa

Rainfall and temperature are two of the major factors triggering malaria epidemics in warm semi-arid (desert-fringe) and high altitude (highland-fringe) epidemic risk areas. The ability of the mosquitoes to transmit Plasmodium spp. is dependent upon a series of biological features generally referred to as vectorial capacity. In this study, the vectorial capacity model (VCAP) was expanded to include the influence of rainfall and temperature variables on malaria transmission potential. Data from two remote sensing products were used to monitor rainfall and temperature and were integrated into the VCAP model. The expanded model was tested in Eritrea and Madagascar to check the viability of the approach. The analysis of VCAP in relation to rainfall, temperature and malaria incidence data in these regions shows that the expanded VCAP correctly tracks the risk of malaria both in regions where rainfall is the limiting factor and in regions where temperature is the limiting factor. The VCAP maps are currently offered as an experimental resource for testing within Malaria Early Warning applications in epidemic prone regions of sub-Saharan Africa. User feedback is currently being collected in preparation for further evaluation and refinement of the VCAP model.

Journal of Tropical Medicine