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BRIDGES: Evolution of basic and applied linkages in benthic science

Growing awareness of environmental degradation resulted in stricter environmental regulations and laws for aquatic ecosystems. These regulations were followed by an increase in applied research and monitoring beginning in the early 1970s. The number of applied scientists who were members of the North American Benthological Society grew at a commensurate rate. The editors of J-NABS recognized that, despite these increases, submitted manuscripts mostly addressed basic science. In response, the BRIDGES section of J-NABS was created in 1994 to provide a forum for linking basic ecological principles to applied science problems and issues. We examined the emergence of applied science topics in J-NABS and its predecessor, Freshwater Invertebrate Biology , from their beginning in 1982 to 2009. We classified papers among 11 categories that included a basic/applied science linkage. In the 1980s, applied papers were predominantly on effects of eutrophication/pollution and landuse changes. When BRIDGES was established in 1994, papers were solicited by editors and BRIDGES sections usually included >1 paper on a common theme to express complementary or alternate viewpoints. Forty-two papers appeared in BRIDGES between 1994 and 2009, but the number per issue declined after 2001. The total number of applied science papers in J-NABS has increased since ∼1994. Citation analysis of BRIDGES papers illustrates how information is being cited, but applied papers often are used in ways that might not lead to citations. BRIDGES transitioned to a new format in September 2009 to address new types of complex, multifaceted linkages. All new BRIDGES articles will be open access, and authors will be encouraged to produce lay-language fact sheets and to post them on the web.

Journal of the North American Benthological Societ↗

Fish and invertebrate flow-biology relationships to support the determination of ecological flows for North Carolina

A method was developed to characterize fish and invertebrate responses to flow alteration in the state of North Carolina. This method involved using 80th percentile linear quantile regressions to relate six flow metrics to the diversity of riffle-run fish and benthic Ephemeroptera, Plecoptera, and Trichoptera (EPT) richness. All twelve flow-biology relationships were found to be significant, with both benthos and fish showing negative responses to ecodeficits and reductions in flow. The responses of benthic richness to reduced flows were consistent and generally greater than that of fish diversity. However, the riffle-run fish guild showed the greatest reductions in diversity in response to summer ecodeficits. The directional consistency and differential seasonal sensitivities of fish and invertebrates to reductions in flow highlight the need to consider seasonality when managing flows. In addition, all relationships were linear, and therefore do not provide clear thresholds to support ecological flow determinations and flow prescriptions to prevent the degradation of fish and invertebrate communities in North Carolina rivers and streams. A method of setting ecological flows based on the magnitude of change in biological condition that is acceptable to society is explored.

North Carolina↗

Variance partitioning of stream diatom, fish, and invertebrate indicators of biological condition

Stream indicators used to make assessments of biological condition are influenced by many possible sources of variability. To examine this issue, we used multiple-year and multiple-reach diatom, fish, and invertebrate data collected from 20 least-disturbed and 46 developed stream segments between 1993 and 2004 as part of the US Geological Survey National Water Quality Assessment Program. We used a variance-component model to summarize the relative and absolute magnitude of 4 variance components (among-site, among-year, site × year interaction, and residual) in indicator values (observed/expected ratio [O/E] and regional multimetric indices [MMI]) among assemblages and between basin types (least-disturbed and developed). We used multiple-reach samples to evaluate discordance in site assessments of biological condition caused by sampling variability. Overall, patterns in variance partitioning were similar among assemblages and basin types with one exception. Among-site variance dominated the relative contribution to the total variance (64–80% of total variance), residual variance (sampling variance) accounted for more variability (8–26%) than interaction variance (5–12%), and among-year variance was always negligible (0–0.2%). The exception to this general pattern was for invertebrates at least-disturbed sites where variability in O/E indicators was partitioned between among-site and residual (sampling) variance (among-site = 36%, residual = 64%). This pattern was not observed for fish and diatom indicators (O/E and regional MMI). We suspect that unexplained sampling variability is what largely remained after the invertebrate indicators (O/E predictive models) had accounted for environmental differences among least-disturbed sites. The influence of sampling variability on discordance of within-site assessments was assemblage or basin-type specific. Discordance among assessments was nearly 2× greater in developed basins (29–31%) than in least-disturbed sites (15–16%) for invertebrates and diatoms, whereas discordance among assessments based on fish did not differ between basin types (least-disturbed = 16%, developed = 17%). Assessments made using invertebrate and diatom indicators from a single reach disagreed with other samples collected within the same stream segment nearly ⅓ of the time in developed basins, compared to ⅙ for all other cases.

Freshwater Science↗

Flow modification in the Nation’s streams and rivers

This report summarizes a national assessment of flowing waters conducted by the U.S. Geological Survey’s (USGS) National Water-Quality Assessment (NAWQA) Project and addresses several pressing questions about the modification of natural flows in streams and rivers. The assessment is based on the integration, modeling, and synthesis of monitoring data collected by the USGS and the U.S. Environmental Protection Agency at more than 7,000 streams and rivers across the conterminous United States from 1980 to 2014. Key findings include the following. First, flow in many of the Nation’s streams and rivers is different from what it would be under natural conditions. In particular, low flows are more frequent, are of shorter duration, and vary less from one year to the next than they would naturally. In addition, high flows have been reduced in magnitude, are of shorter duration, are less frequent, and vary less from one year to the next than they would naturally. Other characteristics of natural flows also have been modified. Second, over the last 60 years (1955–2014), climatic trends have caused a change of 50 percent or more in one or more streamflow attributes at two-thirds of climate-sensitive streamgaging sites. However, these climate-induced changes have been less influential on streamflow modification than have land and water-management practices. Third, in every region assessed, streamflow modification was associated with reduced ecological health, as indicated by two biological communities—invertebrates and fish. Biological communities were increasingly likely to be impaired (defined as having lost a statistically significant number of species) in streams with flows most different from natural conditions. Finally, several case studies are presented that illustrate viable management strategies for balancing the water needs of people and ecosystems.

Circular↗

Physical data and biological data for algae, aquatic invertebrates, and fish from selected reaches on the Carson and Truckee rivers, Nevada and California, 1993-97

This report, a product of the National Water- Quality Assessment Program, is a compilation of physical data and biological data for algae, aquatic invertebrates, and fish collected in the Carson and Truckee River Basins, Nevada and California. Most of the data were collected between 1993 and 1996 at selected reaches on the Carson and Truckee Rivers. Algae and aquatic invertebrate samples were collected from cobble riffles, submerged woody-snag habitats, and from depositional areas such as pools. Between 1993 and 1996, fish and crayfish were collected from all wadeable habitats at each of seven basic-fixed sites using either electroshocking methods or seining. Additional fish and crayfish were collected at one site on the Truckee River in 1997. Fish were identified to species, measured for total and standard length, checked for anomalies, and weighed at the collection site. Fish were returned to the stream after measurements were taken. Measurements of water depth, stream velocity, determinations of substrate type and substrate embeddedness were made at each sampling site. Algae and aquatic invertebrate samples were sent to the U.S. Geological Survey National Water-Quality Laboratory for identification and enumeration. A total of 103 semi-quantitative and 55 qualitative algae samples were collected at 20 river reaches on the Carson and Truckee Rivers between 1993 and 1996. These samples represent algae in cobble riffles, on submerged woody snags, and on sediment surfaces in depositional areas. In those 158 samples, 514 algal species, varieties, or forms were identified. Of the 8 algal phyla represented, the diatoms (Phylum Bacillariophyta) were the most abundant with 351 species, varieties, or forms. The green algae (Phylum Chlorophyta) were next in abundance with 108 species, varieties, or forms followed by the blue-green algae (Phylum Cyanophyta) with 41 species, varieties, or forms. A total of 49 semi-quantitative aquatic invertebrate samples were collected at 27 river reaches on the Carson and Truckee Rivers between 1993 and 1996. These samples represent invertebrates in cobble riffles and on submerged woody snags. In those 49 samples, members of 6 phyla were identified. Roundworms were identified only to phylum (Nematoda) and free-living flatworms and snails were identified only to class (Turbellaria and Gastroda). Organisms were identified as belonging to 19 invertebrate orders. Most of the invertebrates that could be identified to genus or species belonged in the orders Ephemeroptera, Plecoptera, and Trichoptera of the arthropod class Insecta. Fish and crayfish populations in the Carson and Truckee Rivers were sampled 29 times between 1993 and 1997. These collections resulted in the identification of 18 fish species and one endemic crayfish species. Twelve of the 18 fish species identified are not native to the Carson and Truckee River Basins.

Open-File Report↗

The effects of urbanization on the biological, physical, and chemical characteristics of coastal New England streams

During August 2000, responses of biological communities (invertebrates, fish, and algae), physical habitat, and water chemistry to urban intensity were compared among 30 streams within 80 miles of Boston, Massachusetts. Sites chosen for sampling represented a gradient of the intensity of urban development (urban intensity) among drainage basins that had minimal natural variability. In this study, spatial differences were used as surrogates for temporal changes to represent the effects of urbanization over time. The degree of urban intensity for each drainage basin was characterized with a standardized urban index (0-100, lowest to highest) derived from land cover, infrastructure, and socioeconomic variables. Multivariate and multimetric analyses were used to compare urban index values with biological, physical, and chemical data to determine how the data indicated responses to urbanization. Multivariate ordinations were derived for the invertebrate-, fish-, and algae-community data by use of correspondence analysis, and ordinations were derived for the chemical and physical data by use of principal-component analysis. Site scores from each of the ordinations were plotted in relation to the urban index to test for a response. In all cases, the primary axis scores showed the strongest response to the urban index, indicating that urbanization was a primary factor affecting the data ordination. For the multimetric analyses, each of the biological data sets was used to calculate a series of community metrics. For the sets of chemical and physical data, the individual variables and various combinations of individual variables were used as measured and derived metrics, respectively. Metrics that were generally most responsive to the urban index for each data set included: EPT (Ephemeroptera, Plecoptera, Trichoptera) taxa for invertebrates; cyprinid taxa for fish; diatom taxa for algae; bicarbonate, conductivity, and nitrogen for chemistry; and water depth and temperature for physical habitat. The slopes of the responses generally were higher between the urban index values of 0 to 35, indicating that the greatest change in aquatic health may occur between low and moderate levels of urban intensity. Additionally, many of the responses showed that at urban index values greater than 35, there was a threshold effect where the response variable no longer changed with respect to urban intensity. Recognizing and understanding this type of response is important in management and monitoring programs that rely on decisive interpretations of variable responses. Any biological, physical, or chemical variable that is used to characterize stream health over a gradient of disturbance would not be a reliable indicator when a level of disturbance is reached where the variable does not respond in a predictable manner.

Maine, Massachusetts, New Hampshire↗

Magnetic resonance imaging of live freshwater mussels (Unionidae)

We examined the soft tissues of live freshwater mussels, Eastern elliptio Elliptio complanata , via magnetic resonance imaging (MRI), acquiring data with a widely available human whole-body MRI system. Anatomical features depicted in the profile images included the foot, stomach, intestine, anterior and posterior adductor muscles, and pericardial cavity. Noteworthy observations on soft tissue morphology included a concentration of lipids at the most posterior aspect of the foot, the presence of hemolymph-filled fissures in the posterior adductor muscle, the presence of a relatively large hemolymph-filled sinus adjacent to the posterior adductor muscle (at the ventral-anterior aspect), and segmentation of the intestine (a diagnostic description not reported previously in Unionidae). Relatively little is known about the basic biology and ecological physiology of freshwater mussels. Traditional approaches for studying anatomy and tissue processes, and for measuring sub-lethal physiological stress, are destructive or invasive. Our study, the first to evaluate freshwater mussel soft tissues by MRI, clarifies the body plan of unionid mussels and demonstrates the efficacy of this technology for in vivo evaluation of the structure, function, and integrity of mussel soft tissues.

Invertebrate Biology↗

Characterization of the exoskeleton of the Antarctic king crab Paralomis birsteini

Ocean acidification is projected to inhibit the biogenic production of calcium-carbonate skeletons in marine organisms. Antarctic waters represent a natural environment in which to examine the long-term effects of carbonate undersaturation on calcification in marine predators. King crabs (Decapoda: Anomura: Lithodidae), which currently inhabit the undersaturated environment of the continental slope off Antarctica, are potential invasives on the Antarctic shelf as oceanic temperatures rise. Here, we describe the chemical, physical, and mechanical properties of the exoskeleton of the deep-water Antarctic lithodid Paralomis birsteini and compare our measurements with two decapod species from shallow water at lower latitudes: Cancer borealis (Brachyura: Cancridae) and Callinectes sapidus (Brachyura: Portunidae). Paralomis birsteini deposit proportionally more calcium carbonate in their predatory chelae than their protective carapaces, compared with the other two crab species. When exoskeleton thickness and microhardness were compared between the chelae and carapace, the magnitude of the difference between these body regions was significantly greater in P. birsteini than in the other species tested. Hence, there appeared to be a greater disparity in P. birsteini in overall investment in calcium-carbonate structures among regions of the exoskeleton. The imperatives of prey consumption and predator avoidance may be influencing the deposition of calcium to different parts of the exoskeleton in lithodids living in an environment undersaturated with respect to calcium carbonate.

Invertebrate Biology↗

Bacteriological analysis of unionid hemolymph collected from freshwater mussel populations in the Pacific northwestern United States

Native freshwater mussel (Unionidae) mortality events have been occurring with increased frequency in recent decades, with few investigations into potential etiological agents. In the western United States, no surveys have been published regarding the bacteria associated with unionid mussels. Herein, we examine locations of known mussel mortality events in the Chehalis River (Washington), in the Crooked River (Oregon), and Owyhee River (Oregon). Mussel populations considered healthy were sampled in the Skookumchuck River (Washington) for comparison. A variety of bacteria were isolated from these populations, and most notably, Acinetobacter spp. were identified from 82% of moribund individuals of Gonidea angulata in the Owyhee River. Future work evaluating whether Acinetobacter spp. are pathogenic to freshwater mussels could be valuable in unraveling the factors associated with these enigmatic mortality events.

Oregon, Washington↗

Artificial infestation of largemouth bass and walleye with glochidia of Lampsilis ventricosa (Pelecypoda: Unionidae)

The unionid mussel Lampsilis ventricosa is a bundant in the upper Mississippi River and provides a model for studies related to recovery of the endangered Higgins' eye mussel ( Lampsilis higginsi ). We tested the suitability of two species of fish species as hosts for glochidia of L. ventricosa. Laboratory-reared largemouth bass (Micropterus salmoides) and walleyes (Stizostedion vitreum) were artificially infested with the glochidia of L. ventricosa and held separately in two flowthrough tanks for 21 days. Tank bottoms were siphoned daily, and the materials collected were examined for detached glochidia and metamorphosed juveniles. Juveniles were found in both tanks from day 13 post-exposure through day 20, indicating that largemouth bass and walleyes are suitable hosts for the glochidia of L. ventricosa .

Upper Mississippi River↗

The occurrence of oil and the distribution of Hexagenia (Ephemeroptera: Ephemeridae) nymphs in the St. Marys River, Michigan and Ontario

We sampled benthos from the St. Marys River in May 1974 and May 1975 to determine visibly the occurrence of sorpted oil and the distribution of nymphs of the burrowing mayfly Hexagenia in the bottom of the Lake George and Lake Nicolet channels. Results of our survey showed that, between 1967 and 1974-75, the occurrence of oil in the substrate of the Lake George Channel had advanced from 16 km to 30 km downstream from the point where oil is discharged at Sault Ste. Marie, Ontario. Absence or low densities of nymphs coincided with the presence of oil in the sediments. In the Lake Nicolet Channel, evidence of oil pollution was meager and the density of Hexagenia nymphs was generally high.

Freshwater Invertebrate Biology↗

Varechaetadrilus fulleri (Oligochaeta: Tubificidae): New record and amendment of morphological description

The tubificid worm Variechaetadrilus fulleri (Annelida: Oligochaeta) was described by Brinkhurst and Kathman (1983) from the Green River, Kentucky, the only locality from which the species has previously been reported. In 1982-84, a number of specimens of V. fulleri were found in the lower Mississippi River and in an adjacent oxbow lake (Eagle Lake), about 19 river kilometers northwest of Vicksburg, Mississippi. In the lake, the species was collected in the inshore 1-m depth zone but not at 1.6-m depths offshore, where the waters stratified thermally in July and August and the dissolved oxygen concentration sometimes fell to 0.5 mg/liter in the hypolimnion. The collection of sexually mature worms in the river in May 1982 and in Eagle Lake in January-February 1984 enabled us to describe anatomical details that refine the description of the species.

Freshwater Invertebrate Biology↗

Grand Portage Reservation Environmental Monitoring Program

There are seven types of aquatic resources on the Grand Portage Reservation. An ecological monitoring program was proposed for these resources. Some of the resources are pristine, while others have been affected by development. Each type of resource has physical (habitat, sediment, and hydrology), chemical, and biological (fish, invertebrates, and algae) characteristics that are monitored in a consistent manner so that change may be detected. Not all aspects of the physical, chemical, and biotic components are monitored at each resource type. Monitoring is focused on those aspects most susceptible to change. Replication and comparison with pristine components are a significant part of the program so that actual change can be determined from natural temporal and spatial variability.

Minnesota↗

Deep-sea coral research and technology program: Alaska deep-sea coral and sponge initiative final report

Deep-sea coral and sponge ecosystems are widespread throughout most of Alaska’s marine waters. In some places, such as the central and western Aleutian Islands, deep-sea coral and sponge resources can be extremely diverse and may rank among the most abundant deep-sea coral and sponge communities in the world. Many different species of fishes and invertebrates are associated with deep-sea coral and sponge communities in Alaska. Because of their biology, these benthic invertebrates are potentially impacted by climate change and ocean acidification. Deepsea coral and sponge ecosystems are also vulnerable to the effects of commercial fishing activities. Because of the size and scope of Alaska’s continental shelf and slope, the vast majority of the area has not been visually surveyed for deep-sea corals and sponges. NOAA’s Deep Sea Coral Research and Technology Program (DSCRTP) sponsored a field research program in the Alaska region between 2012–2015, referred to hereafter as the Alaska Initiative. The priorities for Alaska were derived from ongoing data needs and objectives identified by the DSCRTP, the North Pacific Fishery Management Council (NPFMC), and Essential Fish Habitat-Environmental Impact Statement (EFH-EIS) process. This report presents the results of 15 projects conducted using DSCRTP funds from 2012-2015. Three of the projects conducted as part of the Alaska deep-sea coral and sponge initiative included dedicated at-sea cruises and fieldwork spread across multiple years. These projects were the eastern Gulf of Alaska Primnoa pacifica study, the Aleutian Islands mapping study, and the Gulf of Alaska fish productivity study. In all, there were nine separate research cruises carried out with a total of 109 at-sea days conducting research. The remaining projects either used data and samples collected by the three major fieldwork projects or were piggy-backed onto existing research programs at the Alaska Fisheries Science Center (AFSC).

NOAA Technical Memorandum↗

Nutrient, Habitat, and Basin-Characteristics Data and Relations with Fish and Invertebrate Communities in Indiana Streams, 1998-2000

An analysis of existing nutrient, habitat, basin-characteristics, and biological-community (fish and invertebrate) data assessed significant relations between nutrients and biological data. Data from 1998 through 2000 for 58 sites in the Upper Wabash River Basin, Lower Wabash River Basin, and tributaries to the Great Lakes and Ohio River Basins were analyzed. Correspondence analysis was used to assess significant relations among nutrients, habitat, basin-characteristics, and biological-community data. Canonical correspondence analysis was used to identify which environmental parameters most influenced the biological communities. When all 58 sites were assessed, six biological-community attributes, metric scores, or site scores were statistically sigificant but weak. When a subset of data was analyzed for eight headwater streams in one ecoregion to minimize the naturally occurring variability associated with the 58 sites, the strength of the relations increased and 24 attributes, metric scores, or site scores were significantly related. Fish-community composition in the 58 sites was most influenced by habitat and land use but not by nutrients. The invertebrate-community composition in the 58 sites was most influenced by habitat, land use, soils, and one nutrient (total Kjeldahl nitrogen [TKN]).

Indiana↗

Relations of principal components analysis site scores to algal-biomass, habitat, basin-characteristics, nutrient, and biological-community data in the Upper Wabash River Basin, Indiana, 2003

Data were gathered from May through October 2003 at 38 randomly selected sites in the Upper Wabash River Basin, Indiana, for algal biomass, habitat, nutrients, and biological communities (fish and invertebrates). Basin characteristics (drainage area and land use) and biological-community attributes and metric scores were determined for each sampling site's basin. Yearly Principal Components Analysis site scores were calculated for algal biomass (periphyton and seston). The yearly Principal Components Analysis site scores for the first axis (PC1) were related, using Spearman's rho, to the seasonal algal-biomass, basin-characteristics, habitat, seasonal nutrient, biological-community attribute and metric score data. The periphyton PC1 site score, which was most influenced by September chlorophyll a, was not related to the five basin-characteristics, nine habitat, or 12 nutrient variables examined. Of the 43 fish-community attributes and metric scores examined, the periphyton PC1 was negatively related to one attribute (tolerant percent) and positively related to one metric score (insectivore percent metric score). Of the 43 invertebrate-community attributes and metric scores examined, the periphyton PC1 was negatively related to three attributes (Ephemeroptera, Plecoptera, and Trichoptera (EPT) count, EPT-to-total ratio, and number of taxa) and four metric scores (EPT count metric score, EPT to total ratio metric score, macroinvertebrate Index of Biotic Integrity metric score, and number of taxa metric score). The seston PC1 was not related to the five basin-characteristics, nine habitat, or 12 nutrient variables or to the 43 fish- and 21 invertebrate-community attributes or metric scores examined. To understand how the choice of sampling sites might have affected the results, an analysis of the drainage area and land use was done. The random site selection in 2003 in the Upper Wabash River Basin was skewed to small streams. The lack of large streams in the Upper Wabash River Basin could account for the few relations among the seston and the biological-community data. The values for nutrients (nitrate, total Kjeldahl nitrogen, total nitrogen, and total phosphorus) and chlorophyll a (periphyton and seston) were compared to published U.S. Environmental Protection Agency (USEPA) values for Aggregate Nutrient Ecoregions VI and VII and USEPA Level III Ecoregions 55 and 56. Several nutrient values were greater than the 25th percentile of the published USEPA values. Chlorophyll a (periphyton and seston) values either were greater than the 25th percentile of published USEPA values or extended data ranges in the Aggregate Nutrient and Level III Ecoregions. If the proposed values for the 25th percentile were adopted as nutrient water-quality criteria, many samples in the Upper Wabash River Basin would have exceeded the criteria.

Indiana↗

Relations of Principal Components Analysis Site Scores to Algal-Biomass, Habitat, Basin-Characteristics, Nutrient, and Biological-Community Data in the West Fork White River Basin, Indiana, 2001

Data were gathered from July through September 2001 at 34 randomly selected sites in the West Fork White River Basin, Indiana for algal biomass, habitat, nutrients, and biological communities (fish and invertebrates). Basin characteristics (drainage area and land use) and biological-community attributes and metric scores were determined for the basin of each sampling site. Yearly Principal Components Analysis site scores were calculated for algal biomass (periphyton and seston). The yearly Principal Components Analysis site scores for the first axis (PC1) were related, using Spearman's rho, to the seasonal algal-biomass, basin-characteristics, habitat, seasonal nutrient, biological-community attribute and metric score data. The periphyton PC1 site score, which was most influenced by ash-free dry mass, was negatively related to one (percent closed canopy) of nine habitat variables examined. Of the 43 fish-community attributes and metric scores examined, the periphyton PC1 was positively related to one fish-community attribute (percent tolerant). Of the 21 invertebrate-community attributes and metric scores examined, the periphyton PC1 was positively related to one attribute (Ephemeroptera, Plecoptera, and Trichoptera (EPT) index) and one metric score (EPT index metric score). The periphyton PC1 was not related to the five basin-characteristic or 12 nutrient variables examined. The seston PC1 site score, which was most influenced by particulate organic carbon, was negatively related to two of the 12 nutrient variables examined: total Kjeldahl nitrogen (July) and total phosphorus (July). Of the 43 fish-community attributes and metric scores examined, the seston PC1 was negatively related to one attribute (large-river percent). Of the 21 invertebrate-community attributes and metric scores examined, the seston PC1 was negatively related to one attribute (EPT-to-total ratio). The seston PC1 was not related to the five basin-characteristics or nine habitat variables examined. To understand how the choice of sampling sites might have affected the results, an analysis of the drainage area and land use was done. The 34 randomly selected sites in the West Fork White River Basin in 2001 were skewed to small streams. The dominant mean land use of the sites sampled was agriculture, followed by forest, and urban. The values for nutrients (nitrate, total Kjeldahl nitrogen, total nitrogen, and total phosphorus) and chlorophyll a (periphyton and seston) were compared to published U.S. Environmental Protection Agency (USEPA) values for Aggregate Nutrient Ecoregions VI and IX and Level III Ecoregions 55 and 72. Several nutrient values were greater than the 25th percentile of the published USEPA values. Chlorophyll a (periphyton and seston) values were either greater than the 25th percentile of published USEPA values or extended data ranges in the Aggregate Nutrient Ecoregions and Level III Ecoregions. If the proposed values for the 25th percentile were adopted as nutrient water-quality criteria, many samples in the West Fork White River Basin would have exceeded the criteria.

Indiana↗

Relations of principal components analysis site scores to algal-biomass, habitat, basin-characteristics, nutrient, and biological-community data in the Whitewater River and East Fork White River basins, Indiana, 2002

Data were gathered from May through September 2002 at 76 randomly selected sites in the Whitewater River and East Fork White River Basins, Indiana, for algal biomass, habitat, nutrients, and biological communities (fish and invertebrates). Basin characteristics (land use and drainage area) and biolog-ical-community attributes and metric scores were determined for the basin of each sampling site. Yearly Principal Compo-nents Analysis site scores were calculated for algal biomass (periphyton and seston). The yearly Principal Components Analysis site scores for the first axis (PC1) were related using Spearman's rho to the seasonal algal-biomass, basin-charac-teristics, habitat, seasonal nutrient, and biological-community attribute and metric score data. The periphyton PC1 site score was not significantly related to the nine habitat or 12 nutrient variables examined. One land-use variable, drainage area, was negatively related to the periphyton PC1. Of the 43 fish-community attributes and metrics examined, the periphyton PC1 was negatively related to one attribute (large-river percent) and one metric score (car-nivore percent metric score). It was positively related to three fish-community attributes (headwater percent, pioneer percent, and simple lithophil percent). The periphyton PC1 was not statistically related to any of the 21 invertebrate-community attributes or metric scores examined. Of the 12 nutrient variables examined two were nega-tively related to the seston PC1 site score in two seasons: total Kjeldahl nitrogen (July and September), and TP (May and September). There were no statistically significant relations between the seston PC1 and the five basin-characteristics or nine habitat variables examined. Of the 43 fish-community attributes and metrics examined, the seston PC1 was positively related to one attribute (headwater percent) and negatively related to one metric score (large-river percent metric score) . Of the 21 invertebrate-community attributes and metrics exam-ined, the seston PC1 was negatively related to one metric score (number of individuals metric score). To understand how the choice of sampling sites might have affected the results, an analysis of the drainage area and land use was done. The sites selected in the Whitewater River Basin were generally small drainage basins; compared to Whitewater River Basin sites, the sites selected in the East Fork White River Basin were generally larger drainage basins. Although both basins were dominated by agricultural land use the Whitewater River Basin sites had more land in agriculture than the East Fork White River Basin sites. The values for nutrients (nitrate, total Kjeldahl nitrogen, total nitrogen, and total phosphorus) and chlorophyll a (per-iphyton and seston) were compared to published U.S. Environmental Protection Agency (USEPA) values for Aggregate Nutrient Ecoregions VI and IX and USEPA Level III Ecore-gions 55 and 71. Several nutrient values were greater than the 25th percentile of published USEPA values. Chlorophyll a (periphyton and seston) values were either greater than the 25thpercentile of published USEPA values or they extended data ranges in the Aggregate Nutrient and Level III Ecore-gions. If the values for the 25th percentile as proposes by the USEPA were adopted as nutrient water-quality criteria, many samples in the Whitewater River and East Fork White River Basins would have exceeded the criteria.

Indiana↗