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At least 19 recordsLinked to original sources

Bird predation on cutworms (Lepidoptera: Noctuidae) in wheat fields and chlorpyrifos effects on brain cholinesterase activity

Horned larks, Eremophila alpestris (L.), and McCown's longspurs, Calcarius mccownii (Lawrence), were collected at intervals from two winter wheat fields in Montana after aerial application of chlorpyrifos to control cutworms. Both bird species had a high (95–100%) incidence of Lepidoptera, mostly pale western cutworms, Agrotis orthogonia Morrison, in their stomachs at 3 days postspray. Incidence of cutworms and other insects in stomachs of birds from sprayed fields was lower at 9 and 16 days postspray than in control birds, presumably due to insecticide-caused reduction of insects. Effects of birds on population dynamics of insect pests in wheat are unknown, but birds do contribute to cutworm mortality. Predation is one of the limiting factors to cutworm increase and can supplement insecticidal control. Brain cholinesterase activity in horned larks collected from the sprayed fields at 3 and 9 days postspray was significantly lower than in unexposed larks, but at 16 days the difference was not significant. Although nontarget birds clearly were exposed to chlorpyrifos and manifested a sublethal physiological response, toxic effects were lesssevere than those resulting from endrin application for cutworm control in wheat. More study is needed of larger chlorpyrifos-treated fields under a variety of conditions to fully assess effects on nontarget life.

Environmental Entomology

Population dynamics of American dog ticks (Acari: Ixodidae) along park trails

We conducted a mark–recapture study in which drag-collected ticks were removed from some park trails weekly from April to July. Weekly survival rates (probability of surviving and remaining on the trails) were significantly lower on trails used heavily by hikers, horses, and pets than on trails used less frequently. Although usage was the only obvious difference among these trails, differences in weekly survival rate estimates may be attributable to differential success in acquiring hosts. The estimated probability of capturing a host-seeking tick located along a trail on a single drag was 0.20 on the drag alone, and 0.25 including the person dragging. When routes parallel to the trails and of equal lengths were dragged immediately after sampling the trails, only ≍5% as many ticks (including ticks on the person dragging) were found off the trails as on them. We found no evidence of reduced tick numbers on removal trails, but this result should be considered inconclusive because the power of the discerning test was low. However, the data reported here provide insights into turnover rates of the adult Dermacentor variabilis population and effectiveness of the drag as a sampling device.

Environmental Entomology

Dietary protein and chlorogenic acid effect on baculoviral disease of noctuid (Lepidoptera: noctuidae) larvae

Insecticidal pathogens such as baculoviruses are currently under intensive development as biorational agents for the control of lepidopteran pests. However, because the efficacy of these orally infective viruses is influenced by host diet, our ability to use baculoviruses effectively in an integrated pest management program requires understanding the influence of dietary components on the disease process. Nutritional stress caused by differences in the quality (casein or soy) or quantity (0–8%) of dietary protein altered the postinfectional course of disease caused by Autographa californica M nucelopolyhedrovirus in 2 species of noctuids, Heliothis virescens (F.) and Trichoplusia ni (Hübner). Lethal times of larvae infected with either the wild-type virus or a recombinant expressing a scorpion toxin (AaIT) derived from this parent virus were similarly affected by dietary protein. In general, the higher the level of dietary protein the shorter the lethal times. However, the influence of protein quality on lethal times depended on the insect species tested. The effect of chlorogenic acid on disease depended on dietary protein levels. At high protein concentrations, chlorogenic acid decreased speed of kill; whereas, at low protein levels, the phenolic had the opposite effect. The common factor among all treatments was that the faster larvae grew, the faster they died from viral infection. We suggest that insects that grow faster may support faster rates of viral replication in infected hosts. From an ecological perspective, it is possible that plants of higher protein content may increase the potential for the development of baculoviral epizootics in insect populations.

Environmental Entomology

Efficacy of Maxforce bait for control of the Argentine ant (Hymenoptera: Formicidae) in Haleakala National Park, Maui, Hawaii

In an effort to develop a chemical control strategy for the invasive Argentine ant, Linepithema humile (Mayr), in Haleakala National Park, Maxforce, which is formulated with 0.9% hydramethylnon, was used in test plots to determine the efficacy of the ant bait in the field. Initially, Maxforce was tested at 2 application rates: broadcast at 2.25 kg/ha (2 lb/acre) and 4.5 kg/ha (4 lb/acre). Later, the following treatments were also tested: a Maxforce and honey granule mix, Maxforce with 0.5% hydramethylnon, Maxforce with a different solvent, Maxforce distributed in exposed piles, and Maxforce distributed in covered piles. Although there were significant differences in the magnitude of ant reduction among the various treatments, all yielded the same general result. Foraging ant numbers at monitoring bait stations declined an average maximum of 97.0% in the test plots, with no plots achieving 100% reduction. At 2 mo after treatment the mean number of foraging ants was reduced by 92.1%. Nest survival in the plots appeared to be affected to a lesser degree, but could not be monitored accurately over the longer term because of the phenomenon of nest movement. A 2nd identical application 1 mo after the initial application in plots treated with Maxforce at 2.25 and 4.5 kg/ha did not result in eradication. Bait molding, quick mortality, and toxicant breakdown from UV radiation created a short exposure time to the bait and toxicant, which may have been the main obstacle to achieving eradication.

Hawaii

Bait preference by the Argentine ant (Hymenoptera: Formicidae) in Haleakala National Park, Hawaii

The Argentine ant, Linepithema humile (Mayr), has proven to be a threat to native arthropod species in Haleakala National Park, Maui, HI, and is also a potential threat to the park's native flora. As it continues to expand its range, an effort has been undertaken to eradicate it, or at the least, control its spread. The 1st part of this effort focused on finding a bait carrier for subsequent toxicant-based control tests. A year-long bait preference test was implemented at each of the ant's 2 infestation sites in Haleakala National Park, in which 6 solid baits and 2 liquid baits were assessed for attractiveness and feasibility for large scale control. At both sites, a toxicant-free formulation of Maxforce, a protein-based granular bait made from ground silkworm, Bombyx mori (L.), pupae, and a 25% sugar water solution were the most attractive baits. Ants took more Maxforce (without toxicant) and sugar water than all other baits, including honey granules and a fish protein bait. Sugar water, however, is difficult to distribute over large natural areas. Maxforce was therefore concluded to be the best bait carrier for toxicant-based control at Haleakala National Park because of its attractiveness and its ease for large scale broadcast dispersal.

Hawaii

Various fish species as biological control agents for the dark rice field mosquito in Arkansas rice fields

During the summers of 1972–73, 10 fish species were evaluated as predators against larvae of the dark rice field mosquito, Psorophora confinnis (Lynch-Arribalzaga), near Stuttgart, Ark. Stocking rates were 0.02, 0.03, and 0.06 fish/ft 2 . At the lowest stocking rate green sunfish, Lepomis cyanellus Rafinesque, reduced larval numbers by 89.7% and were significantly more effective than adult mosquitofish, Gambusia affinis (Baird and Girard). The white amur, Ctenopharynogodon idella Valenciennes, was intermediate in effectiveness when compared with the above. At 0.03 fish/ft 2 , green sunfish reduced larvae by 99.2% and were significantly more efficient than juvenile mosquitofish, which had little effect on the larval population. At the 0.06 fish/ft 2 rate mosquitofish; white amur; Israeli carp, Cyprinus carpio L.; and hybrids (Israeli carp × goldfish) were significantly more effective than bigmouth buffalo, Ictiobus cyprinellus (Valenciennes); black buffalo, Ictiobus niger (Rafinesque); goldfish, Carassius auratus (L.); or juvenile mosquitofish. Recovery counts of fish indicated that fish survived under natural conditions in Arkansas rice fields.

Arkansas

Lead residues in eastern tent caterpillars (Malacosoma americanum) and their host plant (Prunus serotina) close to a major highway

Eastern tent caterpillars, Malacosoma americanum (F.) (Lepidoptera: Lasiocampidae), and leaves of their host plant, black cherry, Prunus serotina Ehrh., were collected in May, 1978, at various distances from the Baltimore-Washington Parkway, Prince George's Co., MD, and were analyzed for lead by atomic absorption spectrophotometry. Caterpillars collected within 10 m of the parkway contained 7.1–7.4 ppm lead (dry weight). Caterpillars collected at greater distances from the parkway and from a control area had lead concentrations ca. half as high (2.6–5.3 ppm). Lead concentrations in caterpillars averaged 76% as high as those in leaves and were much lower than concentrations that have been reported in some roadside soil and litter invertebrates.

Maryland

Non-crop habitat use by wild bees (Hymenoptera: Apoidea) in a mixed-use agricultural landscape

Homogeneous, agriculturally intense landscapes have abundant records of pollinator community research, though similar studies in the forest-dominated, heterogeneous mixed-use landscape that dominates the northeastern United States are sparse. Trends of landscape effects on wild bees are consistent across homogeneous agricultural landscapes, whereas reported studies in the northeastern United States have not found this consistency. Additionally, the role of noncrop habitat in mixed-use landscapes is understudied. We assessed wild bee communities in the mixed-use lowbush blueberry ( Vaccinium angustifolium Ait.) production landscape of Maine, United States at 56 sites in eight land cover types across two regional landscapes and analyzed effects of floral resources, landscape pattern, and spatial scale on bee abundance and species richness. Within survey sites, cover types with abundant floral resources, including lowbush blueberry fields and urban areas, promoted wild bee abundance and diversity. Cover types with few floral resources such as coniferous and deciduous/mixed forest reduced bee abundance and species richness. In the surrounding landscape, lowbush blueberry promoted bee abundance and diversity, while emergent wetland and forested land cover strongly decreased these measures. Our analysis of landscape configuration revealed that patch mixing can promote wild bee abundance and diversity; however, this was influenced by strong variation across our study landscape. More surveys at intra-regional scales may lead to better understanding of the influence of mixed-use landscapes on bee communities.

Environmental Entomology

Capturing spatiotemporal patterns in presence-absence data to inform monitoring and sampling designs for the threatened Dakota skipper (Lepidoptera: Hesperiidae) in the Great Plains of the United States

Declines among species of insect pollinators, especially butterflies, has garnered attention from scientists and managers. Often these declines have spurred governments to declare some species as threatened or endangered. We used existing presence–absence data from surveys for the threatened Dakota skipper Hesperia dacotae (Skinner) to build statistical maps of species presence that could be used to inform future monitoring designs. We developed a hierarchical Bayesian modeling approach to estimate the spatial distribution and temporal trend in Dakota skipper probability of presence. Our model included a spatial random effect and fixed effects for the proportion of two grassland habitat types: those on well-drained soils and those on poorly drained soils; as well as the topographic slope. The results from this model were then used to assess sampling strategies with two different monitoring objectives: locating new Dakota skipper colonies or monitoring the proportion of historically (pre-2000) extant colonies. Our modeling results suggested that the distribution of Dakota skippers followed the distribution of remnant grasslands and that probabilities of presence tended to be higher in topographically diverse grasslands with well-drained soils. Our analysis also showed that the probability of presence declined throughout the northern Great Plains range. Our simulations of the different sampling designs suggested that new detections were expected when sampling where Dakota skippers likely occurred historically, but this may lead to a tradeoff with monitoring existing sites. Prior information about the extant sites may help to ameliorate this tradeoff.

Iowa, Minnesota, North Dakota, South Dakota

Ambrosia beetle (Coleoptera: Curculionidae) communities and frass production in ʻŌhiʻa (Myrtales: Myrtaceae) infected with Ceratocystis (Microascales: Ceratocystidaceae) fungi responsible for Rapid ʻŌhiʻa Death

Rapid ʻŌhiʻa Death (ROD) is a deadly disease that is threatening the native Hawaiian keystone tree species, ʻōhiʻa lehua ( Metrosideros polymorpha Gaudich). Ambrosia beetles (Curculionidae: Scolytinae) and their frass are hypothesized to play a major role in the spread of ROD, although their ecological niches and frass production within trees and across the landscape are not well understood. We characterized the beetle communities and associated frass production from bolts (tree stem sections) representative of entire individual ʻōhiʻa trees from multiple locations across Hawaiʻi Island by rearing beetles and testing their frass for viable ROD-causing fungi. Additionally, we estimated frass production for three beetle species by weighing their frass over time. We found that Xyleborinus saxesenii (Ratzburg), Xyleborus affinis Eichhoff, Xyleborus ferrugineus (Fabricius), Xyleborus perforans (Wollaston), and Xyleborus simillimus Perkins were commonly found on ROD-infected ʻōhiʻa and each produced frass containing viable Ceratocystis propagules. The Hawaiʻi Island endemic beetle and the only native ambrosia beetle associated with ʻōhiʻa, X. simillimus , was limited to high elevations and appeared to utilize similar tree heights or niche dimensions as the invasive X. ferrugineus . Viable Ceratocystis propagules expelled in frass were found throughout entire tree bole sections as high as 13 m. Additionally, we found that X. ferrugineus produced over 4× more frass than X. simillimus . Our results indicate the ambrosia beetle community and their frass play an important role in the ROD pathosystem. This information may help with the development and implementation of management strategies to control the spread of the disease.

Hawaii

Addressing detection uncertainty in Bombus affinis (Hymenoptera: Apidae) surveys can improve inferences made from monitoring

The U.S. Fish and Wildlife Service developed national guidelines to track species recovery of the endangered rusty patched bumble bee [ Bombus affinis Cresson (Hymenoptera: Apidae)] and to investigate changes in species occupancy across space and time. As with other native bee monitoring efforts, managers have specifically acknowledged the need to address species detection uncertainty and determine the sampling effort required to infer species absence within sites. We used single-season, single-species occupancy models fit to field data collected in four states to estimate imperfect detection of B. affinis and to determine the survey effort required to achieve high confidence of species detection. Our analysis revealed a precipitous, seasonal, decline in B. affinis detection probability throughout the July through September sampling window in 2021. We estimated that six, 30-min surveys conducted in early July are required to achieve a 95% cumulative detection probability, whereas >10 surveys would be required in early August to achieve the same level of confidence. Our analysis also showed B. affinis was less likely to be detected during hot and humid days and at patches of reduced habitat quality. Bombus affinis was frequently observed on Monarda fistulosa (Lamiales: Lamiaceae), followed by [ Pycnanthemum virginianum Rob. and Fernald (Lamiales: Lamiaceae)] , Eutrochium maculatum Lamont (Asterales: Asteraceae), and Veronicastrum virginicum Farw. (Lamiales: Plantaginaceae). Although our research is focused on B. affinis , it is relevant for monitoring other bumble bees of conservation concern, such as B. occidentalis Greene (Hymenoptera: Apidae) and B. terricola Kirby (Hymenoptera: Apidae) for which monitoring efforts have been recently initiated and occupancy is a variable of conservation interest.

Illinois, Iowa, Minnesota, Wisconsin

Spatial patterns of seed removal by harvester ants in a seed tray experiment

Using a selection of native grass and forb seeds commonly seeded in local restoration projects, we conducted a field experiment to evaluate the effects of seed species, distance of seed patches from nests, and distance between patches on patterns of seed removal by Owyhee harvester ants, Pogonomyrmex salinus (Olsen) (Hymenoptera: Formicidae). To provide context for ants’ seed preferences, we evaluated differences in handling time among seed species. In addition, we assessed the influences of cheatgrass, Bromus tectorum (L.) (Poales: Poaceae), and Sandberg bluegrass, Poa secunda (J. Presl) (Poales: Poaceae), cover on seed removal. We found significant differences in removal rates among seed species. In general, seeds placed closer to nests were more vulnerable to predation than those placed farther away, and seeds in closely spaced patches were more vulnerable than seeds in widely spaced patches. However, the strength of these effects differed by seed species. Differences in handling time among seed species may help to explain these findings; the protective effect of from-nest distance was weaker for species that required less time to transport. For 2 of the seed species, there was an interaction between the distance of seed patches from nests and the distance between patches such that the protective effect of distance between patches decreased as the distance from nests increased. Cheatgrass and bluegrass cover both had small protective effects on seeds. Taken together, these results offer insight into the spatial ecology of harvester ant foraging and may provide context for the successful implementation of restoration efforts where harvester ants are present.

Environmental Entomology

Mountain pine beetle host selection between lodgepole and ponderosa pines in the southern Rocky Mountains

Recent evidence of range expansion and host transition by mountain pine beetle ( Dendroctonus ponderosae Hopkins; MPB) has suggested that MPB may not primarily breed in their natal host, but will switch hosts to an alternate tree species. As MPB populations expanded in lodgepole pine forests in the southern Rocky Mountains, we investigated the potential for movement into adjacent ponderosa pine forests. We conducted field and laboratory experiments to evaluate four aspects of MPB population dynamics and host selection behavior in the two hosts: emergence timing, sex ratios, host choice, and reproductive success. We found that peak MPB emergence from both hosts occurred simultaneously between late July and early August, and the sex ratio of emerging beetles did not differ between hosts. In two direct tests of MPB host selection, we identified a strong preference by MPB for ponderosa versus lodgepole pine. At field sites, we captured naturally emerging beetles from both natal hosts in choice arenas containing logs of both species. In the laboratory, we offered sections of bark and phloem from both species to individual insects in bioassays. In both tests, insects infested ponderosa over lodgepole pine at a ratio of almost 2:1, regardless of natal host species. Reproductive success (offspring/female) was similar in colonized logs of both hosts. Overall, our findings suggest that MPB may exhibit equally high rates of infestation and fecundity in an alternate host under favorable conditions.

Rocky Mountains

Native bee diversity and pollen foraging specificity in cultivated highbush blueberry (Ericaceae: Vaccinium corymbosum ) in Rhode Island

We identified 41 species of native bees from a total of 1,083 specimens collected at cultivated highbush blueberry plantings throughout Rhode Island in 2014 and 2015. Andrena spp., Bombus spp., and Xylocopa virginica (L.) were collected most often. Bombus griseocollis (DeGeer), B. impatiens Cresson, B. bimaculatus Cresson, B. perplexus Cresson, and Andrena vicina Smith collected the largest mean numbers of blueberry pollen tetrads. The largest mean percent blueberry pollen loads were carried by the miner bees Andrena bradleyi Viereck (91%), A. carolina Viereck (90%), and Colletes validus Cresson (87%). The largest mean total pollen grain loads were carried by B. griseocollis (549,844), B. impatiens (389,558), X. virginica (233,500), and B. bimaculatus (193,132). Xylocopa virginica was the fourth and fifth most commonly collected bee species in 2014 and 2015, respectively. They exhibit nectar robbing and females carried relatively low blueberry pollen loads (mean 33%). Overall, we found 10 species of bees to be the primary pollinators of blueberries in Rhode Island.

Environmental Entomology

MODIS imagery improves pest risk assessment: A case study of wheat stem sawfly (Cephus cinctus, Hymenoptera: Cephidae) in Colorado, USA

Wheat stem sawfly ( Cephus cinctus Norton, Hymenoptera: Cephidae) has long been a significant insect pest of spring, and more recently, winter wheat in the northern Great Plains. Wheat stem sawfly was first observed infesting winter wheat in Colorado in 2010 and, subsequently, has spread rapidly throughout wheat production regions of the state. Here, we used maximum entropy modeling (MaxEnt) to generate habitat suitability maps in order to predict the risk of crop damage as this species spreads throughout the winter wheat-growing regions of Colorado. We identified environmental variables that influence the current distribution of wheat stem sawfly in the state and evaluated whether remotely sensed variables improved model performance. We used presence localities of C. cinctus and climatic, topographic, soils, and normalized difference vegetation index and enhanced vegetation index data derived from Moderate Resolution Imaging Spectroradiometer (MODIS) imagery as environmental variables. All models had high performance in that they were successful in predicting suitable habitat for C. cinctus in its current distribution in eastern Colorado. The enhanced vegetation index for the month of April improved model performance and was identified as a top contributor to MaxEnt model. Soil clay percent at 0–5 cm, temperature seasonality, and precipitation seasonality were also associated with C. cinctus distribution in Colorado. The improved model performance resulting from integrating vegetation indices in our study demonstrates the ability of remote sensing technologies to enhance species distribution modeling. These risk maps generated can assist managers in planning control measures for current infestations and assess the future risk of C. cinctus establishment in currently uninfested regions.

Colorado

A comparison of honey bee-collected pollen from working agricultural lands using light microscopy and ITS metabarcoding

Taxonomic identification of pollen has historically been accomplished via light microscopy but requires specialized knowledge and reference collections, particularly when identification to lower taxonomic levels is necessary. Recently, next-generation sequencing technology has been used as a cost-effective alternative for identifying bee-collected pollen; however, this novel approach has not been tested on a spatially or temporally robust number of pollen samples. Here, we compare pollen identification results derived from light microscopy and DNA sequencing techniques with samples collected from honey bee colonies embedded within a gradient of intensive agricultural landscapes in the Northern Great Plains throughout the 2010–2011 growing seasons. We demonstrate that at all taxonomic levels, DNA sequencing was able to discern a greater number of taxa, and was particularly useful for the identification of infrequently detected species. Importantly, substantial phenological overlap did occur for commonly detected taxa using either technique, suggesting that DNA sequencing is an appropriate, and enhancing, substitutive technique for accurately capturing the breadth of bee-collected species of pollen present across agricultural landscapes. We also show that honey bees located in high and low intensity agricultural settings forage on dissimilar plants, though with overlap of the most abundantly collected pollen taxa. We highlight practical applications of utilizing sequencing technology, including addressing ecological issues surrounding land use, climate change, importance of taxa relative to abundance, and evaluating the impact of conservation program habitat enhancement efforts.

Environmental Entomology

Using publicly available data to quantify plant–pollinator interactions and evaluate conservation seeding mixes in the Northern Great Plains

Concern over declining pollinators has led to multiple conservation initiatives for improving forage for bees in agroecosystems. Using data available through the Pollinator Library (npwrc.usgs.gov/pollinator/), we summarize plant–pollinator interaction data collected from 2012–2015 on lands managed by the U.S. Fish and Wildlife Service and private lands enrolled in U.S. Department of Agriculture conservation programs in eastern North Dakota (ND). Furthermore, we demonstrate how plant–pollinator interaction data from the Pollinator Library and seed cost information can be used to evaluate hypothetical seeding mixes for pollinator habitat enhancements. We summarize records of 314 wild bee and 849 honey bee ( Apis mellifera L.) interactions detected on 63 different plant species. The wild bee observations consisted of 46 species, 15 genera, and 5 families. Over 54% of all wild bee observations were represented by three genera ― Bombus , Lassioglossum , and Melissodes . The most commonly visited forbs by wild bees were Monarda fistulosa , Sonchus arvensis , and Zizia aurea . The most commonly visited forbs by A. mellifera were Cirsium arvense , Melilotus officinalis , and Medicago sativa . Among all interactions, 13% of A. mellifera and 77% of wild bee observations were made on plants native to ND. Our seed mix evaluation shows that mixes may often need to be tailored to meet the unique needs of wild bees and managed honey bees in agricultural landscapes. Our evaluation also demonstrates the importance of incorporating both biologic and economic information when attempting to design cost-effective seeding mixes for supporting pollinators in a critically important part of the United States.

Environmental Entomology

Eastern Carpenter Bee (Hymenoptera: Apidae): Nest structure, nest cell provisions, and trap nest acceptance in Rhode Island

Analysis of pollen provisions in Xylocopa virginica (L.) nests in southern Rhode Island showed that this species produced pollen loaves from 21 different genera of plants in 2016, 19 in 2017, and 39 in 2018. Antirrhinium majus L. (garden snapdragon) pollen was the most common type collected in all three years (21.4%). Overall, wind-pollinated tree pollen comprised 22.1% of all pollen loaves. Blueberry pollen was a minor component of pollen loaves (0.1%), despite abundant blueberry plants nearby. Mean values of X. virginica nest measurements (tunnel length 15.4 ± 1.2 cm, width 15.0 ± 0.5 mm, and cell length 17.7 ± 0.3 mm) were similar to those reported in previous studies. Only 2 of the 216 trap nests deployed in 2017 were occupied by 11 X. virginica bees (9 females and 2 males). However, 17 nests contained 230 Osmia taurus Smith, 6 nests contained 73 O. cornifrons (Radoszkowski), and 1 nest contained 8 O. lignaria Say. Thirty-four nests (15.7%) were occupied by 151 grass-carrying wasps, Isodontia sp. and 6 vespid wasps occupied three nests (1.4%) in 2017. In 2018, 4 of 96 trap nests were occupied by carpenter bees. Understanding the nesting and foraging habits of X. virginica will help us to manage natural populations for pollination services.

Rode Island