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Organic pellet decomposition induces mortality of Lake Trout embryos in Yellowstone Lake

Yellowstone Lake is the site of actions to suppress invasive Lake Trout Salvelinus namaycush and restore native Yellowstone Cutthroat Trout Oncorhynchus clarkii bouvieri and natural ecosystem function. Although gill netting is effective (Lake Trout λ ≤ 0.6 from 2012 through 2018), the effort costs more than US$2 million annually and only targets Lake Trout age 2 and older. To increase suppression efficiency, we developed an alternative method using organic (soy and wheat) pellets to increase mortality of Lake Trout embryos on spawning sites. Decomposition of pellets during two in situ experiments caused dissolved oxygen (DO) concentrations to temporarily decline to lethal levels (<3.4 mg/L) within days of application. Embryo mortalities during the first exposure period (days 1–6 following application) were high at all treatment levels (1.75–28.0 kg/m 2 ) at the substrate surface and within interstices 20 cm below the surface, varying from 97 ± 1.8% (mean ± SE) to 100 ± 0.0%, but may have been enhanced by a handling effect (exposure to sunlight). Embryo mortalities during the second exposure period (days 11–22) were highest 20 cm below the surface, varying from 78 ± 9.7% to 100 ± 0.0%. Almost all (98 ± 3.1%) Lake Trout embryos died after exposure to DO < 3.4 mg/L for >200 h during the second period. Pellets caused lethal DO for several weeks below the substrate surface, despite largely dissolving and dissipating from the surface of treated areas by day 39. Broad-scale application of pellets at 1.75 kg/m 2 following the spawning period in autumn may reduce Lake Trout recruitment and enhance population suppression because the area of 14 verified spawning sites is only 11.4 ha (0.03% of lake surface area). Pellet application may be useful in other similar systems as part of an integrated pest management approach targeting multiple life stages of invasive freshwater fish.

Wyoming↗

Comparison of traditional and geometric morphometrics using Lake Huron ciscoes of the Coregonus artedi complex

Here we determine how traditional morphometrics (TM) compares with geometric morphometrics (GM) in discriminating among morphologies of four forms of ciscoes of the Coregonus artedi complex collected from Lake Huron. One of the forms comprised two groups of the same deepwater cisco separated by capture depth, whereas the other three forms were shallow-water ciscoes. Our three groups of shallow-water ciscoes were better separated (3% versus 19% overlap) in Principle Component Analysis (PCA) with TM data than with GM data incorporating semilandmarks (evenly spaced nonhomologous landmarks used to bridge between widely separated homologous landmarks). Our two deepwater cisco groups, comprising a putatively single form collected from different depths, separated more in PCAs with GM data (33% overlap) than in PCAs with TM data (66% overlap), an anomaly caused by greater decompression of the swimbladder and deformation of the body wall in the group captured at greater depths. Separation of the two deepwater cisco groups captured at different depths was not affected by the removal of semilandmarks. Assignment of forms using canonical variate analysis (CVA) accurately assigned 86% of individuals using TM data, 98% of individuals using GM data incorporating semilandmarks, and 100% of individuals using GM data without semilandmarks. However, we considered assignments from the same form of deepwater cisco into separate groups as misassignments resulting from different capture depths, which reduced the accuracy of assignments with GM data to 66% with semilandmarks. Our study implies that TM will continue to have an important role in morphological discrimination within Coregonus and other fishes similarly shaped.

Lake Huron↗

Sampling characteristics and calibration of snorkel counts to estimate stream fish populations

Snorkeling is a versatile technique for estimating lotic fish population characteristics; however, few investigators have evaluated its accuracy at population or assemblage levels. We evaluated the accuracy of snorkeling using prepositioned areal electrofishing (PAE) for estimating fish populations in a medium-sized Appalachian Mountain river during fall 2008 and summer 2009. Strip-transect snorkel counts were calibrated with PAE counts in identical locations among macrohabitats, fish species or taxa, and seasons. Mean snorkeling efficiency (i.e., the proportion of individuals counted from the true population) among all taxa and seasons was 14.7% (SE, 2.5%), and the highest efficiencies were for River Chub Nocomis micropogon at 21.1% (SE, 5.9%), Central Stoneroller Campostoma anomalum at 20.3% (SE, 9.6%), and darters (Percidae) at 17.1% (SE, 3.7%), whereas efficiencies were lower for shiners ( Notropis spp., Cyprinella spp., Luxilus spp.) at 8.2% (SE, 2.2%) and suckers (Catostomidae) at 6.6% (SE, 3.2%). Macrohabitat type, fish taxon, or sampling season did not significantly explain variance in snorkeling efficiency. Mean snorkeling detection probability (i.e., probability of detecting at least one individual of a taxon) among fish taxa and seasons was 58.4% (SE, 6.1%). We applied the efficiencies from our calibration study to adjust snorkel counts from an intensive snorkeling survey conducted in a nearby reach. Total fish density estimates from strip-transect counts adjusted for snorkeling efficiency were 7,288 fish/ha (SE, 1,564) during summer and 15,805 fish/ha (SE, 4,947) during fall. Precision of fish density estimates is influenced by variation in snorkeling efficiency and sample size and may be increased with additional sampling effort. These results demonstrate the sampling properties and utility of snorkeling to characterize lotic fish assemblages with acceptable efficiency and detection probability, less effort, and no mortality, compared with traditional sampling methods.

North American Journal of Fisheries Management↗

Considerations for using tag-returns to monitor targeted removal of invasive fishes

Objective Targeted removals are used for management of some invasive fish populations. Tag–return studies are one approach that can be used to assess the efficacy of targeted removals. However, there are many decisions to make when designing a tag–return study. We used simulation modeling to outline general guidelines for consideration when designing efficient tag–return studies to measure annual removal rates of invasive fish, particularly invasive carps. Methods We simulated data sets using scenarios with varying numbers of fish tagged per year, removal rates, tag reporting rates, tag retention rates, and study durations. We generated the data sets under a set of “known” parameters with added stochasticity; we then fitted the simulated data sets to a Bayesian tag–return model and measured the precision and accuracy of the model-estimated removal rates. Results We found that the model was able to predict removal rates without bias for most of the scenarios. However, we did find patterns in the precision of the predictions that could help to inform tag–return studies. When the proportion of the population removed through harvest was constant, the proportion of the population removed per year and the probability that harvested tags were reported had the largest effect on precision. The number of tags released per year and the study duration also had moderate effects. For scenarios testing the ability of the model to predict removal rates in stochastic populations, the precision of the model was primarily influenced by the number of fish tagged, the underlying nature of the stochasticity, and whether fish were tagged during the year of the prediction. Conclusions Based on our simulations, we outline how study objectives, the underlying population variability, and the tolerance range for error can guide decisions regarding the number of fish to tag, how to monitor tag return rates, and how long to conduct a study.

North American Journal of Fisheries Management↗

Persistence of native riverine fishes downstream from two hydropower dams with contrasting operations

Identifying hydropower dam operations that lessen detrimental effects on downstream fauna could inform conservation strategies for native fishes. We compared occurrence of native fishes in 20 shoal habitats downstream from two differently operated hydropower dams in the Coosa River system, Georgia, USA. Species richness averaged 7 and 11, respectively, in surveys downstream from (1) a hydropeaking dam and (2) a dam with a re-regulation structure that stabilized downstream flows. In contrast, surveys in two nearby reference communities averaged 19 and 24 species. Species persisting downstream from the dams tended toward water-column orientation, larger body size, longer life-span, and greater prevalence in tributary stream collections, compared with missing or rarely captured species. We observed no evidence of recovery toward reference conditions when operations were paused for 28 months at the hydropeaking dam. Our observations suggest that (1) strongly contrasting dam operations can result in similar alterations to native fish assemblages, potentially reflecting effects of thermal alteration by hypolimnetic water release, and (2) periodic dispersal from tributary streams may enhance fish persistence in flow-altered rivers.

Canadian Journal of Fisheries and Aquatic Sciences↗

Vulnerability to predation and physiological stress responses in juvenile chinook salmon ( Oncorhynchus tshawytscha ) experimentally infected with Renibacterium salmoninarum

We experimentally infected juvenile chinook salmon ( Oncorhynchus tshawytscha ) with Renibacterium salmoninarum (Rs), the causative agent of bacterial kidney disease (BKD), to examine the vulnerability to predation of fish with differing levels of Rs infection and assess physiological change during progression of the disease. Immersion challenges conducted during 1992 and 1994 produced fish with either a low to moderate (1992) or high (1994) infection level of Rs during the 14-week postchallenge rearing period. When equal numbers of treatment and unchallenged control fish were subjected to predation by either northern squaw fish ( Ptychocheilus oregonensis ) or smallmouth bass ( Micropterus dolomieui) , Rs-challenged fish were eaten in significantly greater numbers than controls by nearly two to one. In 1994, we also sampled fish every 2 weeks after the challenge to determine some stressful effects of Rs infection. During disease progression in fish, plasma cortisol and lactate increased significantly whereas glucose decreased significantly. Our results indicate the role that BKD may play in predator-prey interactions, thus ascribing some ecological significance to this disease beyond that of direct pathogen-related mortality. In addition, the physiological changes observed in our fish during the chronic progression of BKD indicate that this disease is stressful, particularly during the later stages.

Canadian Journal of Fisheries and Aquatic Sciences↗

Comparison of protection in rainbow trout (Salmo gairdneri) inoculated with and fed Hagerman redmouth bacterins

Rainbow trout ( Salmo gairdneri ) fed 1.0 mg Hagerman redmouth bacterin per fish for 2 wk had no detectable specific, circulating, agglutinating antibody. In fish given a single subcutaneous inoculation of 1.0 mg of bacterin per fish, antibody was present from 3 wk later until 3 mo later, when the final sample was taken. Median lethal doses at various intervals after the bacterins were administered indicated that the inoculated fish could withstand a greater challenge by subcutaneous inoculation of the virulent bacteria than the orally immunized fish. The fish fed the vaccine lost their protection within 6 wk, whereas the inoculated fish had high levels of protection through 3 mo. The degree of protection was also confirmed by a "natural" exposure challenge.

Journal of the Fisheries Research Board of Canada↗

Amino acid supplementation of casein in diets of Atlantic salmon (Salmo salar) fry and of soybean meal for rainbow trout (Salmo gairdneri) fingerlings

Fry of Atlantic salmon ( Salmo salar ) fed a diet containing isolated fish protein grew significantly faster than those fed a diet containing casein without supplemental amino acids, and had improved feed conversion and lower mortality. Supplementing the casein diet with essential amino acids to the levels in the isolated fish protein diet significantly increased growth rate, reduced mortality, and improved feed conversion.When fingerling rainbow trout ( S . gairdneri ) were fed diets containing soybean meal as the sole source of protein, additions of amino acids to simulate those levels of essential amino acids in trout eggs and isolated fish protein significantly improved growth. Additions of methionine, lysine, histidine, and leucine individually and in several combinations had no effect.

Journal of the Fisheries Research Board of Canada↗

Effect of capture stress on plasma enzyme activities in rainbow trout (Salmo gairdneri)

Four capture methods were used to collect domesticated rainbow trout ( Salmo gairdneri ): angling, electroshocking, seining, and direct netting (control). Blood was sampled rapidly upon capture, usually within 2&ensp;min. No significant differences were noted within the time frame of the experiment between the four capture groups for plasma protein concentration, lactate dehydrogenase activity, or leucine aminonaphthylamidase activity. Creatine phosphokinase activity was elevated among electroshocked fish. Acid phosphatase activity was too low for accurate measurement. Hematocrits were significantly elevated by capture struggles. These results indicate that these capture methods do not preclude the use of plasma enzyme levels for investigating the health of wild fish. Key words : plasma enzyme, capture stress, physiology, plasma protein, rainbow trout, lactate dehydrogenase, leucine aminonaphthylamidase, creatine phosphokinase

Journal of the Fisheries Research Board of Canada↗

Acoustic measures of the abundance and size of pelagic planktivores in Lake Michigan

Based on acoustic data taken at night and vertically stratified by bottom depth (3&ndash;110 m only), the total number (&plusmn; 95% Cl) of pelagic fishes in Lake Michigan was 43.4 &plusmn; 10.1 x 10 9 or 226.0 &plusmn; 55.2 kt in spring (mean density 0.7&ndash;3.8 fish&middot;m -2 or 1.6&ndash;12.8 ga&middot;m -2 ) and 115.8 &plusmn; 18.3 x 109 or 313.2 &plusmn; 74.3 kt in late summer, 1987 (mean density 1.1&ndash;7.0 fish&middot;m -2 or 3.0&ndash;13.2 g&middot;m -2 ); approximately 30% of this increase in numbers (35% of biomass) occurred within Green Bay. Abundance estimates from horizontally stratified (by water column depth) data were within 9-11% of vertically stratified estimates during spring but over 20% higher during summer. By extrapolation to all water depths, we estimated total pelagic biomass as 274.6 kt for spring and 410.8 kt for summer. During both seasons, smaller fishes were nearer to the surface and nearer shore than larger individuals, and acoustic measures of size approximated the sizes of fishes caught in trawls. Bioenergetic model simulations suggest that 60% of the available production of alewife (Alosa pseudoharengus) was either consumed by stocked salmonines (52.9%) or commercially harvested (7.1%) in 1987. Underwater acoustics proved a valuable tool for lakewide assessments of fish abundances in the Great Lakes.

Michigan↗

Tumor frequencies in walleye ( Stizostedion vitreum ) and brown bullhead ( Ictalurus nebulosus ) and sediment contaminants in tributaries of the Laurentian Great Lakes

To better characterize neoplasm epizootics in the Great Lakes basin and their association with families of contaminants, we sampled five locations: the Fox and Menominee rivers, Lake Michigan; Munuscong Lake, St. Mary's River; and the Black and Cuyahoga rivers, Lake Erie. Frequencies of external and liver tumors were determined for brown bullhead ( Ictalurus nebulosus ) from all locations except the Black River and for walleye ( Stizostedion vitreum ) from the Lake Michigan and St. Mary's River sites. Sediment samples were analyzed for metals, polychlorinated aromatics, and polynuclear aromatic hydrocarbons (PAH). Liver neoplasms occurred in brown bullhead from the Cuyahoga River and Munuscong Lake; brown bullhead captured from Munuscong Lake were older than those collected from the other locations. Brown bullhead from these same two rivers had elevated hepatosomatic indexes. No liver neoplasms were found in brown bullhead from the Fox and Menominee rivers, although polychlorinated aromatics were highest in both Fox River sediment and Fox and Menominee brown bullhead, and arsenic was highest in Menominee River sediment and fish. Liver neoplasms in brown bullhead from the Cuyahoga River fit the prevailing hypothesis that elevated PAH in sediment can induce cancer in wild fish. The cause of the liver neoplasms in Munuscong Lake brown bullhead is undetermined.

Canadian Journal of Fisheries and Aquatic Sciences↗

Modification of the microhematocrit technique with trout blood

Commercial and laboratory‐prepared capillary tubes for hematocrit were tested. A 10 percent solution of heparin was found to be best for the preparation of treated capillary tubes. For examination of trout blood laboratory‐prepared capillary tubes were found to be superior to commercial tubes which are designed for human blood. Sharp, pointed heparinized capillary tubes were used with good results for collecting blood from the heart without killing the examined fish. Hematocrits determined directly in such tubes were found to have the same value as those prepared in other ways.

Transactions of the American Fisheries Society↗

Effects of an aquatic plant and suspended clay on the activity of fish toxicants

Rotenone, antimycin, permethrin, pydrin, and Salicylanilide I were tested for their toxicities against fathead minnows ( Pimephales promelas ) in the presence of Canadian waterweed ( Elodea canadensis ) or suspended clay. The plants had little effect on the activity of rotenone and antimycin but substantially reduced the activity of permethrin and pydrin (synthetic pyrethroids). Bentonite severely inhibited the activity of all chemicals tested. Salicylanilide I was affected least and pydrin most (27 times as much pydrin was required when I g/liter of bentonite was present in 96-hour tests). The efficacy of the registered fish toxicants rotenone and antimycin is probably not significantly affected by vegetation under field conditions, but is greatly reduced by suspended bentonite clay.

North American Journal of Fisheries Management↗

Diet and feeding periodicity of ruffe in the St. Louis River estuary, Lake Superior

Ruffe Gymnocephalus cernuus , a percid native to Europe and Asia, is established in the Lake Superior drainage and could have negative impacts on native fish through competition for forage and predation on fish eggs. We investigated the diet of ruffes in the 4,654-ha St. Louis River estuary in May&ndash;October 1989&ndash;1990 and the feeding periodicity of ruffes in two adjacent habitats during five 24-h periods in summers 1990&ndash;1991. Ruffes were primarily benthophagous. Age-0 ruffes fed mostly on cladocerans and copepods in early summer and midge larvae (Chironomidae) in late summer and fall. Adult ruffes less than 12 cm fed mostly on midges and other macrobenthos but also consumed large numbers of microcrustaceans. Adult ruffes 12 cm and larger fed mostly on midges, burrowing mayflies Hexagenia spp., and caddisflies (Trichoptera). Ruffes consumed few fish eggs. Adult ruffes in deeper waters and all age-0 ruffes fed throughout the day as indicated by weight patterns of stomach contents. However, adult ruffes generally moved to shallower waters at night to feed most heavily. Results of this study indicate that ruffes will probably compete with other benthic-feeding fishes such as yellow perch Perca flavescens and trout-perch Percopsis omiscomaycus .

Transactions of the American Fisheries Society↗

Ontogenic and spatial patterns in diet and growth of lake trout in Lake Michigan

Lake trout Salvelinus namaycush in nearshore waters of Lake Michigan grow faster than lake trout residing offshore on Sheboygan Reef, which is in midlake. We examined the stomachs of lake trout, spanning ages 1 through 16, caught in both nearshore and offshore environments of Lake Michigan during 1994 and 1995 to determine whether diet differences may be responsible for the difference in growth rate. A comparison of the diets, coupled with bioenergetics modeling, indicated that juvenile lake trout on Sheboygan Reef experienced slow growth due to low food availability rather than to cold water temperatures. The availability of appropriate‐size prey appeared to regulate lake trout growth. Small prey fish were probably not readily available to small (200‐ to 399‐mm total length) lake trout on Sheboygan Reef, a substantial portion of whose diet consisted of invertebrates; in contrast, nearshore juveniles had a nearly 100% fish diet. Growth rate on the reef remained slow through intermediate lake trout sizes (400–599 mm total length), presumably due to low availability of rainbow smelt Osmerus mordax on the reef. Once lake trout achieved total lengths of approximately 600 mm, they grew slightly faster on Sheboygan Reef than near shore, indicating that large (>170‐mm total length) prey fish were readily available to lake trout in the reef area. On a wet‐weight basis, alewife Alosa pseudoharengus dominated the diet of large (≥600 mm total length) lake trout from both the nearshore and offshore regions of the lake, although bloater Coregonus hoyi composed over 30% of the diet on Sheboygan Reef and in southeastern nearshore Lake Michigan. Size of alewife prey increased with lake trout size. The bloater population currently represents the bulk of the biomass of the adult prey fish community, so our diet analysis suggests that large lake trout are continuing to select alewives.

Illinois, Indiana, Michigan, Wisconsin↗

Transferability of habitat suitability criteria for fishes in warmwater streams

We developed habitat suitability criteria and tested their transferability for nine fishes inhabiting unregulated Piedmont and Coastal Plain streams in Alabama. Criteria for optimal habitat were defined as those ranges of depth, velocity, substrate type, and cover type for which a species' suitability index (proportional abundance divided by proportional habitat availability, scaled from 0 to I) equalled or exceeded 0.4. We evaluated the transferability of criteria between study sites by testing the null hypothesis that species occurrence in a sample was independent of whether or not the sample was taken in optimal habitat. We also tested criteria transference to a large, flow‐regulated river sampled during low‐flow periods. Depth, velocity, and most substrate criteria developed for the bronze darter Percina palmaris successfully transferred between unregulated streams and to the flow‐regulated river samples. All criteria developed for a pair of closely related, allopatric darter species. the newly described lipstick darter Etheostoma chuckwachatte and the greenbreast darter E. jordani , transferred successfully when applied between species (in the unregulated sites) and to the regulated river samples. In contrast, criteria for the Alabama shiner Cyprinella callistia failed nearly all tests of transferability. Criteria for the speckled darter E. stigmaeum , the blackbanded darter P. nigrofasciata , an undescribed Percina species, and a pair of related, allopatric Cyprinella species transferred inconsistently. The species with good criteria transference had high suitability indices for the shallow depths. fast current velocities. and coarse substrates characteristic of riffle species. We suggest that microhabitat criteria for riffle fishes are more likely to provide a transferable measure of habitat quality than criteria for fishes that, although restricted to fluvial habitats, commonly occupy a variety of pool and riffle habitats.

North American Journal of Fisheries Management↗

Thermal marking of Atlantic salmon otoliths

By exposing fry of Atlantic salmon Salmo salar to various temperature regimes, we determined conditions required to produce a readable thermal otolith mark. Marks were quantified by using a darkness index that represented the number of times darker the mark was than the background. Minimum requirements for a strong mark were 1 d at a relatively warm premark temperature of 9°C and 8 h at a lower marking temperature (1°C). Temperature decreases of 4°C (from 5°C to 1°C) resulted in strong marks if fish were held at the premark temperature for 4 d and at the marking temperature for at least 24 h.

Vermont↗