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Geographic variation in patterns of nestedness among local stream fish assemblages in Virginia

Nestedness of faunal assemblages is a multiscale phenomenon, potentially influenced by a variety of factors. Prior small-scale studies have found freshwater fish species assemblages to be nested along stream courses as a result of either selective colonization or extinction. However, within-stream gradients in temperature and other factors are correlated with the distributions of many fish species and may also contribute to nestedness. At a regional level, strongly nested patterns would require a consistent set of structuring mechanisms across streams, and correlation among species' tolerances of the environmental factors that influence distribution. Thus, nestedness should be negatively associated with the spatial extent of the region analyzed and positively associated with elevational gradients (a correlate of temperature and other environmental factors). We examined these relationships for the freshwater fishes of Virginia. Regions were defined within a spatial hierarchy and included whole river drainages, portions of drainages within physiographic provinces, and smaller subdrainages. In most cases, nestedness was significantly stronger in regions of smaller spatial extent and in regions characterized by greater topographic relief. Analysis of hydrologic variability and patterns of faunal turnover provided no evidence that interannual colonization/extinction dynamics contributed to elevational differences in nestedness. These results suggest that, at regional scales, nestedness is influenced by interactions between biotic and abiotic factors, and that the strongest nestedness is likely to occur where a small number of organizational processes predominate, i.e., over small spatial extents and regions exhibiting strong environmental gradients. ?? Springer-Verlag 2004.

Oecologia↗

Can parasites be indicators of free-living diversity? Relationships between species richness and the abundance of larval trematodes and of local benthos and fishes

Measuring biodiversity is difficult. This has led to efforts to seek taxa whose species richness correlates with the species richness of other taxa. Such indicator taxa could then reduce the time and cost of assessing the biodiversity of the more extensive community. The search for species richness correlations has yielded mixed results, however. This may be primarily because of the lack of functional relationships between the taxa studied. Trematode parasites are highly promising bioindicators. Diverse assemblages of larval trematode parasites are easily sampled in intermediate host snails. Through their life cycles these parasites are functionally coupled with the surrounding free-living diversity of vertebrate and invertebrate animals. It has been shown that larval trematodes in snails correlate positively with bird diversity and abundance. Here, we explore whether trematodes also correlate with standard measures of fishes, and large and small benthos, for 32 sites in three wetlands. We found associations between trematodes and benthic communities that were not consistent across wetlands. The associations were, however, consistently positive for large benthic species richness and density. Some of the contrasting associations between trematode and benthos may be explained by negative associations between large and small benthos. We found no associations with fish communities (probably because of the inadequacy of standard "snapshot" sampling methods for highly mobile fishes). The results support further exploration of trematodes as bioindicators of diversity and abundance of animal communities. ?? 2006 Springer-Verlag.

Oecologia↗

Genetic diversity and species diversity of stream fishes covary across a land-use gradient

Genetic diversity and species diversity are expected to covary according to area and isolation, but may not always covary with environmental heterogeneity. In this study, we examined how patterns of genetic and species diversity in stream fishes correspond to local and regional environmental conditions. To do so, we compared population size, genetic diversity and divergence in central stonerollers ( Campostoma anomalum ) to measures of species diversity and turnover in stream fish assemblages among similarly sized watersheds across an agriculture–forest land-use gradient in the Little Miami River basin (Ohio, USA). Significant correlations were found in many, but not all, pair-wise comparisons. Allelic richness and species richness were strongly correlated, for example, but diversity measures based on allele frequencies and assemblage structure were not. In-stream conditions related to agricultural land use were identified as significant predictors of genetic diversity and species diversity. Comparisons to population size indicate, however, that genetic diversity and species diversity are not necessarily independent and that variation also corresponds to watershed location and glaciation history in the drainage basin. Our findings demonstrate that genetic diversity and species diversity can covary in stream fish assemblages, and illustrate the potential importance of scaling observations to capture responses to hierarchical environmental variation. More comparisons according to life history variation could further improve understanding of conditions that give rise to parallel variation in genetic diversity and species diversity, which in turn could improve diagnosis of anthropogenic influences on aquatic ecosystems.

Ohio↗

New parasites and predators follow the introduction of two fish species to a subarctic lake: implications for food-web structure and functioning

Introduced species can alter the topology of food webs. For instance, an introduction can aid the arrival of free-living consumers using the new species as a resource, while new parasites may also arrive with the introduced species. Food-web responses to species additions can thus be far more complex than anticipated. In a subarctic pelagic food web with free-living and parasitic species, two fish species (arctic charr Salvelinus alpinus and three-spined stickleback Gasterosteus aculeatus ) have known histories as deliberate introductions. The effects of these introductions on the food web were explored by comparing the current pelagic web with a heuristic reconstruction of the pre-introduction web. Extinctions caused by these introductions could not be evaluated by this approach. The introduced fish species have become important hubs in the trophic network, interacting with numerous parasites, predators and prey. In particular, five parasite species and four predatory bird species depend on the two introduced species as obligate trophic resources in the pelagic web and could therefore not have been present in the pre-introduction network. The presence of the two introduced fish species and the arrival of their associated parasites and predators increased biodiversity, mean trophic level, linkage density, and nestedness; altering both the network structure and functioning of the pelagic web. Parasites, in particular trophically transmitted species, had a prominent role in the network alterations that followed the introductions.

Takvatn Lake↗

Multiple hypotheses testing of fish incidence patterns in an urbanized ecosystem

Ecological and evolutionary theories have focused traditionally on natural processes with little attempt to incorporate anthropogenic influences despite the fact that humans are such an integral part of virtually all ecosystems. A series of alternate models that incorporated anthropogenic factors and traditional ecological mechanisms of invasion to account for fish incidence patterns in urban lakes was tested. The models were based on fish biology, human intervention, and habitat characteristics. However, the only models to account for empirical patterns were those that included fish invasiveness, which incorporated species-specific information about overall tolerance and fecundity. This suggests that species-specific characteristics are more important in general distributional patterns than human-mediated dispersal. Better information of illegal stocking activities is needed to improve human-mediated models, and more insight into basic life history of ubiquitous species is needed to truly understand underlying mechanisms of biotic homogenization. ?? Springer 2005.

Aquatic Ecology↗

Carbon dioxide as an under-ice lethal control for invasive fishes

Resource managers need effective tools to control invasive fish populations. In this study, we tested under-ice carbon dioxide (CO 2 ) injection as a novel piscicide method for non-native Silver Carp ( Hypophthalmichthys molitrix ), Bighead Carp ( Hypophthalmichthys nobilis ), Grass Carp ( Ctenopharyngodon idella ), Common Carp ( Cyprinus carpio ) and native Bigmouth Buffalo ( Ictiobus cyprinellus ). Fish were held overwinter in nine outdoor ponds (0.04 ha surface area; 340,000 L volume) treated with no CO 2 (control), 43.5–44.0 kg CO 2 (low treatment), and 87.5–88.5 kg CO 2 (high treatment). Ponds were harvested immediately after ice-out to assess survival and condition. Resulting survival in low (mean = 32%) and high (mean = 5%) CO 2 -treated ponds was significantly lower than untreated control ponds (mean = 84%). Lethal efficacy varied across species with no Bighead Carp, Silver Carp, or Bigmouth Buffalo surviving the high CO 2 treatment. External infections were observed more frequently after CO 2 treatments (means = 49–67%) relative to untreated ponds (mean = 2%), suggesting a secondary mechanism for poor survival. This study demonstrates that CO 2 can be used as a lethal control for invasive fishes, but effectiveness may vary by species and CO 2 concentration.

Biological Invasions↗

High densities of conspecifics buffer native fish from negative interactions with an ecologically similar invasive

Invasive species are a leading cause for native species declines, but it remains unclear whether maintenance of high native densities influence native persistence in freshwater systems. We designed complementary laboratory and field experiments to test whether high native cutthroat trout (Oncorhynchus clarkii) densities affect competition with invasive brown trout (Salmo trutta). We manipulated density of native cutthroat trout while holding brown trout density constant. Interspecific aggressive interactions towards native cutthroat trout were 66% fewer in the highest cutthroat trout density treatment compared to the lowest density treatment. At high densities of conspecifics, cutthroat trout, sympatric with brown trout, lost 33-81% less weight and demonstrated 1.5 times greater survival in both experiments than at low conspecific densities. Cutthroat trout held at low densities in laboratory experiments experienced greater stress-related disease virulence and negative effects of harassment, apparently owing to more frequent aggressive interactions with brown trout. These results support the hypothesis that reduced per capita effects of invaders on native fish at high densities buffers native species from negative effects of invaders, reducing the potential for invasive fish to displace high density native fish populations.

Utah↗

Predicting invasiveness of freshwater fishes imported into North America: Regional differences in models and outcomes

Biological invasions driven by international trade heighten the urgency for development of invasion risk models, as the traits and parameters that consistently predict successful invasion remain unresolved. For four regions of North America that include parts of the United States and Canada (Sacramento-San Joaquin River Basins, Lower Colorado River Basin, Great Lakes Region, Mid-Atlantic Region), we construct and compare classification tree models to reveal robust predictors for the establishment and ecological impact stages of freshwater fish invasion. We subsequently apply the models to identify invasive fish species in trade and conduct pathway analyses to determine which trades (aquarium, biological supply, live bait, live food, water garden) and source continents pose the greatest risk to each region. Model results differed by invasion stage and region. Across regions, establishment models shared climate-related predictors including climate match and temperature tolerance. Three of the four impact models contained prior establishment success. The greatest number of species (548) were predicted to establish in the Sacramento-San Joaquin while the fewest (5) were predicted to establish in the Mid-Atlantic. Forty species were predicted to establish in multiple regions, five of which were also predicted to have high impact. The aquarium trade and Asia supplied the most species predicted to establish. Taken together, the results highlight region-specific models, indicating no universal model predicts invasion. Climate-related and prior establishment variables were most useful to risk assessments. The regional models, and identified high-risk pathways and potential invaders, could be applied to prevent future fish invasions in North America.

Laurentian Great Lakes Region, Lower Colorado Rive↗

Isolation by a hydroelectric dam induces minimal impacts on genetic diversity and population structure in six fish species

Reduced connectivity created by artificial barriers can influence the genetic integrity of isolated subpopulations by reducing local population sizes and altering patterns of gene flow. We investigated the genetic impacts of one such barrier, the Prairie du Sac dam, Wisconsin, USA, using microsatellite data from six fish species with varying life history traits sampled above and below the dam. Contrary to many past studies in other systems, we did not detect any significant differences in genetic diversity between populations found above and below the Prairie du Sac dam. Our results also revealed low genetic differentiation ( F ST = 0–0.008) between populations above and below the dam for all species. In fact, we found that more genetic variation was partitioned among sampling years than between above and below dam populations for all but one of the species. Results from coalescent simulations designed to model our study system indicated that the genetic impacts of the dam will likely be detectable approximately 40–60 generations after the dam was constructed, and that it is possible to largely mitigate these impacts with a fish passage strategy that facilitates a migration rate of ≥ 1% between above and below dam populations. In summary, our findings suggest the genetic impacts of dams can be relatively minimal on short time scales, and that fish passage strategies can significantly reduce genetic impacts if designed appropriately.

Wisconsin↗

Deformity, erosion, lesion, tumor, and parasite (DELT) anomalies in fish communities of the Chesapeake Bay watershed, USA: A regional assessment and potential landscape drivers

Fish diseases in freshwater ecosystems pose significant ecological and socioeconomic challenges, yet monitoring them in wild populations is complex due to interactions between pathogens, hosts, and environmental conditions. We examine the prevalence and watershed-scale landscape drivers of external deformity, erosion, lesion, tumor, and parasite (DELT) anomalies in 57 riverine fish species using a large dataset (577,266 individuals collected 2008–2019) from the Chesapeake Bay watershed that originated from state and federal agencies. Overall, DELT prevalence was low (1.4%), but was higher in larger, longer-lived species, including Channel Catfish ( Ictalurus punctatus ) (18.9%), Rock Bass ( Ambloplites rupestris ) (7.6%), Smallmouth Bass ( Micropterus dolomieu ) (7.3%), Brown Bullhead ( Ameiurus nebulosus ) (5.6%), and Yellow Bullhead ( Ameiurus natalis ) (5.1%), signifying their potential as regional environmental health indicators. Spatial analysis indicated warmer temperatures increased the estimated probability of DELT occurrence, whereas higher precipitation often mitigated the probability of DELT occurrence. Conservation strategies (e.g., best management practices) had mixed effectiveness in reducing DELT occurrence probability across agricultural and urban landscapes. Across the landscape, various drivers, including harvested forest, impervious land, and pesticide use, influenced DELT occurrence probability differently across species. However, uncertainty remains partly due to low prevalence and variability in sampling methods across agencies. Despite low overall prevalence, DELT occurrence is a rapid fish health indicator. Future research could emphasize species-specific responses and longitudinal studies that incorporate life stages and health indicators. Understanding these intricate, multi-scale interactions is vital for effective monitoring, conservation, and adaptive management of freshwater ecosystems.

Chesapeake Bay watershed↗

Hydrologic regimes as potential drivers of morphologic divergence in fish

Fishes often exhibit phenotypic divergence across gradients of abiotic and biotic selective pressures. In streams, many of the known selective pressures driving phenotypic differentiation are largely influenced by hydrologic regimes. Because flow regimes drive so many attributes of lotic systems, we hypothesized fish exhibit phenotypic divergence among streams with different flow regimes. We used a comparative field study to investigate the morphological divergence of Campostoma anomalom (central stonerollers) among streams characterized by highly variable, intermittent flow regimes and streams characterized by relatively stable, groundwater flow regimes. We also conducted a mesocosm experiment to compare the plastic effects of one component of flow regimes, water velocity, on morphology of fish from different flow regimes. We observed differences in shape between flow regimes likely driven by differences in allometric growth patterns. Although we observed differences in morphology across flow regimes in the field, C. anomalum did not exhibit morphologic plasticity in response to water velocity alone. This study contributes to the understanding of how complex environmental factors drive phenotypic divergence and may provide insight into the evolutionary consequences of disrupting natural hydrologic patterns, which are increasingly threatened by climate change and anthropogenic alterations.

Evolutionary Ecology↗

Life histories, salinity zones, and sublethal contributions of contaminants to pelagic fish declines illustrated with a case study of San Francisco Estuary, California, USA

Human effects on estuaries are often associated with major decreases in abundance of aquatic species. However, remediation priorities are difficult to identify when declines result from multiple stressors with interacting sublethal effects. The San Francisco Estuary offers a useful case study of the potential role of contaminants in declines of organisms because the waters of its delta chronically violate legal water quality standards; however, direct effects of contaminants on fish species are rarely observed. Lack of direct lethality in the field has prevented consensus that contaminants may be one of the major drivers of coincident but unexplained declines of fishes with differing life histories and habitats (anadromous, brackish, and freshwater). Our review of available evidence indicates that examining the effects of contaminants and other stressors on specific life stages in different seasons and salinity zones of the estuary is critical to identifying how several interacting stressors could contribute to a general syndrome of declines. Moreover, warming water temperatures of the magnitude projected by climate models increase metabolic rates of ectotherms, and can hasten elimination of some contaminants. However, for other pollutants, concurrent increases in respiratory rate or food intake result in higher doses per unit time without changes in the contaminant concentrations in the water. Food limitation and energetic costs of osmoregulating under altered salinities further limit the amount of energy available to fish; this energy must be redirected from growth and reproduction toward pollutant avoidance, enzymatic detoxification, or elimination. Because all of these processes require energy, bioenergetics methods are promising for evaluating effects of sublethal contaminants in the presence of other stressors, and for informing remediation. Predictive models that evaluate the direct and indirect effects of contaminants will be possible when data become available on energetic costs of exposure to contaminants given simultaneous exposure to non-contaminant stressors.

California↗

Effects of host injury on susceptibility of marine reef fishes to ectoparasitic gnathiid isopods

The importance of the role that parasites play in ecological communities is becoming increasingly apparent. However much about their impact on hosts and thus populations and communities remains poorly understood. A common observation in wild populations is high variation in levels of parasite infestation among hosts. While high variation could be due to chance encounter, there is increasing evidence to suggest that such patterns are due to a combination of environmental, host, and parasite factors. In order to examine the role of host condition on parasite infection, rates of Gnathia marleyi infestation were compared between experimentally injured and uninjured fish hosts. Experimental injuries were similar to the minor wounds commonly observed in nature. The presence of the injury significantly increased the probability of infestation by gnathiids. However, the level of infestation (i.e., total number of gnathiid parasites) for individual hosts, appeared to be unaffected by the treatment. The results from this study indicate that injuries obtained by fish in nature may carry the additional cost of increased parasite burden along with the costs typically associated with physical injury. These results suggest that host condition may be an important factor in determining the likelihood of infestation by a common coral reef fish ectoparasite, G. marleyi .

Symbiosis↗

The oxidation of drugs by fishes

1. Fish liver microsomal systems have been found to catalyze the hydroxylation of aniline and acetanilide, the N-demethylation of aminopyrine and the O-dealkylation of phenacetin. 2. These systems are similar to the corresponding mammalian enzymes and they may be considered to be mixed function oxidase since they require NADPH and oxygen. An absolute requirement for oxygen, however, was difficult to demonstrate for the hepatic phenacetin cleavage system from fish. 3. Microsomal drug metabolizing systems from fish have temperature optima which are considerably lower than those of corresponding mammalian systems

Comparative Biochemistry and Physiology, Part A: M↗

Irradiation of fish fillets: Relation of vapor phase reactions to storage quality

Fish fillets irradiated under air, nitrogen, oxygen, or carbon dioxide atmospheres developed rancidlike flavors when they were stored at refrigerated temperatures. Packing and irradiating under vacuum or helium prevented development of off-flavors during storage. Significant quantities of nitrate and oxidizing substances were formed when oxygen, nitrogen, or air were present in the vapor or liquid phases contained in a Pyrex glass model system exposed to ionizing radiation supplied by a 60 Co source. It was demonstrated that the delayed flavor changes that occur in stored fish fillets result from the reaction of vapor phase radiolysis products and the fish tissue substrates.

International Journal of Applied Radiation and Iso↗

Effects of kelp forest removal on associated fish assemblages in central California

Visual surveys along subtidal belt transects were used to compare fish assemblages on an experimental and a control site before and after the removal of a canopy-forming kelp forest. The giant kelp Macrocystis pyrifera (L.) C.A. Agardh was removed at the holdfast from ≈ 1 ha of high relief structurally complex rock substratum. The abundance of seven species of fish, of which five were considered midwater species, significantly declined after the kelp was removed. No increases in abundance were noted within the experimental site. On the control site, two declines in abundance were observed, while two species increased in abundance. These results indicate that the presence of a giant kelp forest may increase the abundance and species diversity of the fish assemblages over a high relief rocky reef in central California, U.S.A.

Journal of Experimental Marine Biology and Ecology↗

Test system for exposing fish to resuspended, contaminated sediment

We describe a new test system for exposing fish to resuspended sediments and associated contaminants. Test sediments were resuspended by revolving test chambers on rotating shafts driven by an electric motor. The timing, speed, and duration of test-chamber revolution were controlled by a rheostat and electronic timer. Each chamber held 45 litres of water and accommodated about 49 g of test fish. The system described had three water baths, each holding six test chambers. We illustrate the performance of this system with results from a 28-day test in which juvenile bluegills Lepomis macrochirus were exposed to resuspended, riverine sediments differing in texture and cadmium content. The test had one sediment-free control and five sediment treatments, with three replicates (chambers) per treatment and 25 fish per replicate. Two-thirds (30 litres) of the test water and sediment in each chamber was renewed weekly. The mean concentration of total suspended solids (TSS) did not vary among treatments; the grand-mean TSS in the five sediment treatments was 975 mg litre −1 , similar to the target TSS of 1000 mg litre −1 . At the end of the test, an average of 50% of the introduced cadmium was associated with the suspended sediment compartment, whereas the filtered (0.45 μm) water contained 0.4% and bluegills 1.8% of the cadmium.

Environmental Pollution↗

Measurements of the abilities of cultured fishes to moisturize their digesta

1. Four salmonid and four cool-water fish species were tested to determine their ability to moisturize their digesta. 2. After the fish were fed, they were sacrificed, the gut contents were removed and water content was determined. 3. The digesta of the salmonids contained the least water (63–72%) and those of largemouth bass the most (78%). 4. We conclude that there are distinct and significant differences between species and genera in the ability of fish to moisturize their digesta. The potential significance of this finding is discussed.

Comparative Biochemistry and Physiology, Part A: P↗