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Evaluating intercepts from demographic models to understand resource limitation and resource thresholds

Understanding resource limitation is critical to effective management and conservation of wild populations, however resource limitation is difficult to quantify partly because resource limitation is a dynamic process. Specifically, a resource that is limiting at one time may become non-limiting at another time, depending upon changes in its availability and changes in the availability of other resources. Methods for understanding resource limitation, therefore, must consider the dynamic effects of resources on demography. We present approaches for interpreting results of demographic modeling beyond analyzing model rankings, model weights, slope estimates, and model averaging. We demonstrate how interpretation of y-intercepts, odds ratios, and rates of change can yield insights into resource limitation as a dynamic process, assuming logistic regression is used to link estimates of resources with estimates of demography. In addition, we show how x-intercepts can be evaluated with respect to odds ratios to understand resource thresholds. ?? 2007 Elsevier B.V. All rights reserved.

Ecological Modelling↗

Soil nutrient-landscape relationships in a lowland tropical rainforest in Panama

Soils play a crucial role in biogeochemical cycles as spatially distributed sources and sinks of nutrients. Any spatial patterns depend on soil forming processes, our understanding of which is still limited, especially in regards to tropical rainforests. The objective of our study was to investigate the effects of landscape properties, with an emphasis on the geometry of the land surface, on the spatial heterogeneity of soil chemical properties, and to test the suitability of soil-landscape modeling as an appropriate technique to predict the spatial variability of exchangeable K and Mg in a humid tropical forest in Panama. We used a design-based, stratified sampling scheme to collect soil samples at 108 sites on Barro Colorado Island, Panama. Stratifying variables are lithology, vegetation and topography. Topographic variables were generated from high-resolution digital elevation models with a grid size of 5 m. We took samples from five depths down to 1 m, and analyzed for total and exchangeable K and Mg. We used simple explorative data analysis techniques to elucidate the importance of lithology for soil total and exchangeable K and Mg. Classification and Regression Trees (CART) were adopted to investigate importance of topography, lithology and vegetation for the spatial distribution of exchangeable K and Mg and with the intention to develop models that regionalize the point observations using digital terrain data as explanatory variables. Our results suggest that topography and vegetation do not control the spatial distribution of the selected soil chemical properties at a landscape scale and lithology is important to some degree. Exchangeable K is distributed equally across the study area indicating that other than landscape processes, e.g. biogeochemical processes, are responsible for its spatial distribution. Lithology contributes to the spatial variation of exchangeable Mg but controlling variables could not be detected. The spatial variation of soil total K and Mg is mainly influenced by lithology. ?? 2007 Elsevier B.V. All rights reserved.

Forest Ecology and Management↗

Assessing accuracy and precision for field and laboratory data: a perspective in ecosystem restoration

Unlike most laboratory studies, rigorous quality assurance/quality control (QA/QC) procedures may be lacking in ecosystem restoration (“ecorestoration”) projects, despite legislative mandates in the United States. This is due, in part, to ecorestoration specialists making the false assumption that some types of data (e.g. discrete variables such as species identification and abundance classes) are not subject to evaluations of data quality. Moreover, emergent behavior manifested by complex, adapting, and nonlinear organizations responsible for monitoring the success of ecorestoration projects tend to unconsciously minimize disorder, QA/QC being an activity perceived as creating disorder. We discuss similarities and differences in assessing precision and accuracy for field and laboratory data. Although the concepts for assessing precision and accuracy of ecorestoration field data are conceptually the same as laboratory data, the manner in which these data quality attributes are assessed is different. From a sample analysis perspective, a field crew is comparable to a laboratory instrument that requires regular “recalibration,” with results obtained by experts at the same plot treated as laboratory calibration standards. Unlike laboratory standards and reference materials, the “true” value for many field variables is commonly unknown. In the laboratory, specific QA/QC samples assess error for each aspect of the measurement process, whereas field revisits assess precision and accuracy of the entire data collection process following initial calibration. Rigorous QA/QC data in an ecorestoration project are essential for evaluating the success of a project, and they provide the only objective “legacy” of the dataset for potential legal challenges and future uses.

Restoration Ecology↗

Resilience of Alaska’s boreal forest to climatic change

This paper assesses the resilience of Alaska’s boreal forest system to rapid climatic change. Recent warming is associated with reduced growth of dominant tree species, plant disease and insect outbreaks, warming and thawing of permafrost, drying of lakes, increased wildfire extent, increased postfire recruitment of deciduous trees, and reduced safety of hunters traveling on river ice. These changes have modified key structural features, feedbacks, and interactions in the boreal forest, including reduced effects of upland permafrost on regional hydrology, expansion of boreal forest into tundra, and amplification of climate warming because of reduced albedo (shorter winter season) and carbon release from wildfires. Other temperature-sensitive processes for which no trends have been detected include composition of plant and microbial communities, long-term landscape-scale change in carbon stocks, stream discharge, mammalian population dynamics, and river access and subsistence opportunities for rural indigenous communities. Projections of continued warming suggest that Alaska’s boreal forest will undergo significant functional and structural changes within the next few decades that are unprecedented in the last 6000 years. The impact of these social–ecological changes will depend in part on the extent of landscape reorganization between uplands and lowlands and on policies regulating subsistence opportunities for rural communities.

Alaska↗

Landscape matrix mediates occupancy dynamics of Neotropical avian insectivores

In addition to patch‐level attributes (i.e., area and isolation), the nature of land cover between habitat patches (the matrix) may drive colonization and extinction dynamics in fragmented landscapes. Despite a long‐standing recognition of matrix effects in fragmented systems, an understanding of the relative impacts of different types of land cover on patterns and dynamics of species occurrence remains limited. We employed multi‐season occupancy models to determine the relative influence of patch area, patch isolation, within‐patch vegetation structure, and landscape matrix on occupancy dynamics of nine Neotropical insectivorous birds in 99 forest patches embedded in four matrix types (agriculture, suburban development, bauxite mining, and forest) in central Jamaica. We found that within‐patch vegetation structure and the matrix type between patches were more important than patch area and patch isolation in determining local colonization and local extinction probabilities, and that the effects of patch area, isolation, and vegetation structure on occupancy dynamics tended to be matrix and species dependent. Across the avian community, the landscape matrix influenced local extinction more than local colonization, indicating that extinction processes, rather than movement, likely drive interspecific differences in occupancy dynamics. These findings lend crucial empirical support to the hypothesis that species occupancy dynamics in fragmented systems may depend greatly upon the landscape context.

Clarendon Parish, Manchester Parish↗

Hydrologic impacts of thawing permafrost—A review

Where present, permafrost exerts a primary control on water fluxes, flowpaths, and distribution. Climate warming and related drivers of soil thermal change are expected to modify the distribution of permafrost, leading to changing hydrologic conditions, including alterations in soil moisture, connectivity of inland waters, streamflow seasonality, and the partitioning of water stored above and below ground. The field of permafrost hydrology is undergoing rapid advancement with respect to multiscale observations, subsurface characterization, modeling, and integration with other disciplines. However, gaining predictive capability of the many interrelated consequences of climate change is a persistent challenge due to several factors. Observations of hydrologic change have been causally linked to permafrost thaw, but applications of process-based models needed to support and enhance the transferability of empirical linkages have often been restricted to generalized representations. Limitations stem from inadequate baseline permafrost and unfrozen hydrogeologic characterization, lack of historical data, and simplifications in structure and process representation needed to counter the high computational demands of cryohydrogeologic simulations. Further, due in part to the large degree of subsurface heterogeneity of permafrost landscapes and the nonuniformity in thaw patterns and rates, associations between various modes of permafrost thaw and hydrologic change are not readily scalable; even trajectories of change can differ. This review highlights promising advances in characterization and modeling of permafrost regions and presents ongoing research challenges toward projecting hydrologic and ecologic consequences of permafrost thaw at time and spatial scales that are useful to managers and researchers.

Vadose Zone Journal↗

Scale-appropriate adaptation strategies and actions in the Northeast and Midwest United States

Climate Change Adaptation is a growing field within conservation and natural resource management. Actions taken toward climate change adaptation account for climate impacts and ecological responses, both current and projected into the future. These actions attempt to accomplish a number of goals, including the conservation of wildlife and ecosystems by reducing vulnerability and increasing resilience. Climate change adaptation strategies and approaches for natural resources can be thought of as part of a continuum of potential actions ranging from 1) options or goals to 2) strategies, 3) approaches, and 4) tactics. There are a range of decision support tools and processes to aid climate change adaptation. This document highlights several including the Adaptation Workbook, Climate Change Vulnerability Assessments, Structured Decision Making, Adaptive Resource Management, and Scenario Planning. It will also provide case studies on the application of these tools across the Northeast and Midwest. Improved, better-integrated, and increasingly coordinated monitoring systems would be helpful to detect, track, and attribute species and habitat shifts to climate change over spatiotemporal scales. We highlight regional examples of projects and programs addressing these challenges. Illustrative case studies of climate change adaptation efforts are presented across landscape/ecoregion, state, and local scales. Appendix 4.1 provides a synthesis of over 900 general, species and habitat-specific adaptation strategies and tactics from 9 regional studies being considered or implemented across the region. The study of climate change adaptation is a relatively new and rapidly growing field focused on preparing for and responding to the current and future impacts of climate change. The goal of this chapter is to highlight different approaches, processes, and tools currently being used across the region through illustrative case studies at varying scales. In addition, we provide a synthesis of numerous species and habitat-specific adaptation strategies and actions from existing assessment reports and management plans, which is intended to showcase a range of possibilities for natural resource management under future global change. This report does not prescribe one specific approach to taking action; instead, we outline a range of adaptation 5 tactics, which will require thoughtful consideration of the needs of the species, habitat, and location, the stakeholders and partners involved, the scale that a decision or policy is being implemented at, and the financial and personnel resources available to managers.

Connecticut, Delaware, Iowa, Illinois, Indiana, Ke↗

Recent research on the hydrodynamics of the Sacramento - San Joaquin River Delta and north San Francisco Bay

This article presents an overview of recent findings from hydrodynamic research on circulation and mixing in the Sacramento-San Joaquin Delta (Delta) (Figure 1) and North San Francisco Bay (North Bay) (Figure 2). For the purposes of this article, North Bay includes San Pablo Bay, Carquinez Strait, and Suisun Bay. The findings presented are those gained from field studies carried out by the U.S. Geological Survey (USGS), as part of the Interagency Ecological Program (IEP), and Stanford University beginning about 1993. The premise behind these studies was that a basic understanding of circulation and mixing patterns in the Bay and Delta is an essential part of understanding how biota and water quality are affected by natural hydrologic variability, water appropriation, and development activities. Data collected for the field studies described in this article have significantly improved our understanding of Bay and Delta hydrodynamics. Measured flows ,in the Delta have provided valuable information on how water moves through the Delta's network of channels and how export pumping affects flows. Studies of the shallows and shallow-channel exchange processes conducted in Honker Bay have shown that the water residence time in Honker Bay is much shorter than previously reported (on the order of hours to several tidal cycles instead ofweeks). Suisun Bay studies have provided data on hydrodynamic transport and accumulation mechanisms that operate primarily in the channels. The Suisun Bay studies have caused us to revise our understanding of residual circulation in the channels of North Bay and of "entrapment" mechanisms in the low salinity zone. Finally, detailed tidal and residual (tidally averaged) time-scale studies of the mechanisms that control gravitational circulation in the estuary show that density-driven transport in the channels is governed by turbulence time-scale (seconds) interactions between the mean flow and stratification. The hydrodynamic research summarized in this article spans a range of estuarine environments (deep water channels to shallow water habitats and brackish water to freshwater) at time scales that range from seconds to years.

California↗

Optimization of salt marsh management at the Edwin B. Forsythe National Wildlife Refuge, New Jersey, through use of structured decision making

Structured decision making is a systematic, transparent process for improving the quality of complex decisions by identifying measurable management objectives and feasible management actions; predicting the potential consequences of management actions relative to the stated objectives; and selecting a course of action that maximizes the total benefit achieved and balances tradeoffs among objectives. The U.S. Geological Survey, in cooperation with the U.S. Fish and Wildlife Service, applied an existing, regional framework for structured decision making to develop a prototype tool for optimizing tidal marsh management decisions at the Edwin B. Forsythe National Wildlife Refuge in New Jersey. Refuge biologists, refuge managers, and research scientists identified multiple potential management actions to improve the ecological integrity of 23 marsh management units within the refuge and estimated the outcomes of each action in terms of performance metrics associated with each management objective. Value functions previously developed at the regional level were used to transform metric scores to a common utility scale, and utilities were summed to produce a single score representing the total management benefit that could be accrued from each potential management action. Constrained optimization was used to identify the set of management actions, one per marsh management unit, that could maximize total management benefits at different cost constraints at the refuge scale. Results indicated that, for the objectives and actions considered here, total management benefits may increase consistently up to about \$980,000, but that further expenditures may yield diminishing return on investment. Potential management actions in optimal portfolios at total costs less than \$980,000 included applying sediment to the marsh surface to increase elevation in five marsh management units, digging runnels on the marsh surface to improve drainage in five marsh management units, and breaching roads and berms to improve tidal flow in five marsh management units. The potential management benefits were derived from expected reduction in the duration of surface flooding, improved capacity for marsh elevation to keep pace with sea-level rise and increases in numbers of spiders (as an indicator of trophic health), tidal marsh obligate birds, and wintering American black ducks. The prototype presented here does not resolve management decisions; rather, it provides a framework for decision making at the Edwin B. Forsythe National Wildlife Refuge that can be updated as new data and information become available. Insights from this process may also be useful to inform future habitat management planning at the refuges.

New Jersey↗

Broad-scale impacts of an invasive native predator on a sensitive native prey species within the shifting avian community of the North American Great Basin

Human enterprise has modified ecosystem processes through direct and indirect alteration of native predators’ distribution and abundance. For example, human activities subsidize food, water, and shelter availability to generalist predators whose subsequent increased abundance impacts lower trophic-level prey species. The common raven (Corvus corax; hereafter, raven) is an avian predator, native to the northern hemisphere, that can become invasive when subsidized. Raven populations are increasing at unprecedented rates in many regions globally. Information regarding scale of impact and potential ecological thresholds is needed to guide conservation actions aimed at reducing adverse effects on sensitive prey. We conducted a multi-part analysis to investigate broad-scale variation in raven densities and impacts on nesting greater sage-grouse (Centrocercus urophasianus), an indicator species for sagebrush ecosystems in western North America. We estimated raven densities using 16,000 point surveys over 10 years within the Great Basin, USA, and examined associations with anthropogenic and environmental covariates. Average density was 0.54 ravens km-2 (95% CI: 0.42–0.70), with higher densities at lower relative elevations comprising increased agriculture and development. We then used a reduced dataset to estimate the effect of raven density on sage-grouse nest survival (nests = 737). We identified negative impacts to nesting sage-grouse, especially where raven density exceeded ~ 0.40 km-2, a potential ecological threshold. We mapped regions where elevated raven densities were predicted to depress sage-grouse population growth in the absence of compensatory demographic responses from other sage-grouse life-history stages, and found ~ 64% of sage-grouse breeding areas were adversely impacted by high raven density.

California, Idaho, Nevada, Oregon, Utah↗

Depth-dependent soil mixing persists across climate zones

Soil mixing over long (>10 2 y) timescales enhances nutrient fluxes that support soil ecology, contributes to dispersion of sediment and contaminated material, and modulates fluxes of carbon through Earth’s largest terrestrial carbon reservoir. Despite its foundational importance, we lack robust understanding of the rates and patterns of soil mixing, largely due to a lack of long-timescale data. Here we demonstrate that luminescence, a light-sensitive property of minerals used for geologic dating, can be used as a long-timescale sediment tracer in soils to reveal the structure of soil mixing. We develop a probabilistic model of transport and mixing of tracer particles and associated luminescence in soils and compare with a global compilation of luminescence versus depth in various locations. The model–data comparison reveals that soil mixing rate varies over the soil depth, with this depth dependency persisting across climate and ecological zones. The depth dependency is consistent with a model in which mixing intensity decreases linearly or exponentially with depth, although our data do not resolve between these cases. Our findings support the long-suspected idea that depth-dependent mixing is a spatially and temporally persistent feature of soils. Evidence for a climate control on the patterns and intensities of soil mixing with depth remains elusive and requires the further study of soil mixing processes.

PNAS↗

A new groundwater energy transport model for the MODFLOW hydrologic simulator

Heat transport in the subsurface is an important aspect of research related to the effects of a warming climate on ecological services (i.e., cold-water refugia); the development of geothermal resources for energy banking schemes (i.e., aquifer thermal energy storage [ATES]); and the effects of temperature on other aspects of groundwater quality, such as nutrient cycling. Historically, simulation of heat transport using the MODFLOW groundwater simulator and related codes was performed by scaling the input parameters of a solute-transport model to emulate heat transport. However, that approach required additional pre- and post-processing of input and output and could not account for the variation in effective thermal storage and transport properties during transient, unsaturated flow, for example. True heat-transport capabilities in the context of MODFLOW were first introduced in a variant called USG-Transport. More recently, a new groundwater energy-transport (GWE) model type has been added to MODFLOW 6, the core version of the MODFLOW hydrologic simulator. GWE supports the simulation of heat transport on structured or unstructured grids as well as within and between features of advanced packages that represent streams, lakes, multi-aquifer wells, and the unsaturated zone. GWE is integrated within MODFLOW 6 and is accessible through the FloPy Python package and the MODFLOW 6 application programming interface (API). An example simulation demonstrates conduction between grid cells through both the water and the solid aquifer material, including thermal bleeding from saturated overburden cells into a groundwater flow field.

Groundwater↗

Climate change: evaluating your local and regional water resources

The BCM is a fine-scale hydrologic model that uses detailed maps of soils, geology, topography, and transient monthly or daily maps of potential evapotranspiration, air temperature, and precipitation to generate maps of recharge, runoff, snow pack, actual evapotranspiration, and climatic water deficit. With these comprehensive environmental inputs and experienced scientific analysis, the BCM provides resource managers with important hydrologic and ecologic understanding of a landscape or basin at hillslope to regional scales. The model is calibrated using historical climate and streamflow data over the range of geologic materials specific to an area. Once calibrated, the model is used to translate climate-change data into hydrologic responses for a defined landscape, to provide managers an understanding of potential ecological risks and threats to water supplies and managed hydrologic systems. Although limited to estimates of unimpaired hydrologic conditions, estimates of impaired conditions, such as agricultural demand, diversions, or reservoir outflows can be incorporated into the calibration of the model to expand its utility. Additionally, the model can be linked to other models, such as groundwater-flow models (that is, MODFLOW) or the integrated hydrologic model (MF-FMP), to provide information about subsurface hydrologic processes. The model can be applied at a relatively small scale, but also can be applied to large-scale national and international river basins.

Fact Sheet↗

Framework for a hydrologic climate-response network in New England

Many climate-related hydrologic variables in New England have changed in the past century, and many are expected to change during the next century. It is important to understand and monitor these changes because they can affect human water supply, hydroelectric power generation, transportation infrastructure, and stream and riparian ecology. This report describes a framework for hydrologic monitoring in New England by means of a climate-response network. The framework identifies specific inland hydrologic variables that are sensitive to climate variation; identifies geographic regions with similar hydrologic responses; proposes a fixed-station monitoring network composed of existing streamflow, groundwater, lake ice, snowpack, and meteorological data-collection stations for evaluation of hydrologic response to climate variation; and identifies streamflow basins for intensive, process-based studies and for estimates of future hydrologic conditions.

Conecticut, Maine, Massachusetts, New Hampshire, R↗

Optimization of tidal marsh management at the Cape May and Supawna Meadows National Wildlife Refuges, New Jersey, through use of structured decision making

Structured decision making is a systematic, transparent process for improving the quality of complex decisions by identifying measurable management objectives and feasible management actions; predicting the potential consequences of management actions relative to the stated objectives; and selecting a course of action that maximizes the total benefit achieved and balances tradeoffs among objectives. The U.S. Geological Survey, in cooperation with the U.S. Fish and Wildlife Service, applied an existing, regional framework for structured decision making to develop a prototype tool for optimizing tidal marsh management decisions at the Cape May and Supawna Meadows National Wildlife Refuges in New Jersey. Refuge biologists, refuge managers, and research scientists identified multiple potential management actions to improve the ecological integrity of 13 marsh management units within the refuges and estimated the outcomes of each action in terms of performance metrics associated with each management objective. Value functions previously developed at the regional level were used to transform metric scores to a common utility scale, and utilities were summed to produce a single score representing the total management benefit that would be accrued from each potential management action. Constrained optimization was used to identify the set of management actions, one per marsh management unit, that would maximize total management benefits at different cost constraints at the refuge scale. Results indicated that, for the objectives and actions considered here, total management benefits may increase consistently up to approximately $ 785,000, but that further expenditures may yield diminishing return on investment. Management actions in optimal portfolios at total costs less than $ 785,000 included applying sediment to the marsh surface (thin layer deposition) in seven marsh management units, controlling the invasive reed Phragmites australis in four marsh management units, remediating hydrologic alterations in two marsh management units, and planting native vegetation in one marsh management unit. The management benefits were derived from expected improvements in the capacity for marsh elevation to keep pace with sea-level rise, increases in numbers of spiders (as an indicator of trophic health) and tidal marsh obligate birds, and increased cover of native vegetation. The prototype presented here provides a framework for decision making at the Cape May and Supawna Meadows National Wildlife Refuges that can be updated as new data and information become available. Insights from this process may also be useful to inform future habitat management planning at the refuges.

New Jersey↗

Measuring the relative resilience of subarctic lakes to global change: redundancies of functions within and across temporal scales

1. Ecosystems at high altitudes and latitudes are expected to be particularly vulnerable to the effects of global change. We assessed the responses of littoral invertebrate communities to changing abiotic conditions in subarctic Swedish lakes with long-term data (1988–2010) and compared the responses of subarctic lakes with those of more southern, hemiboreal lakes. 2. We used a complex systems approach, based on multivariate time-series modelling, and identified dominant and distinct temporal frequencies in the data; that is, we tracked community change at distinct temporal scales. We determined the distribution of functional feeding groups of invertebrates within and across temporal scales. Within and cross-scale distributions of functions have been considered to confer resilience to ecosystems, despite changing environmental conditions. 3. Two patterns of temporal change within the invertebrate communities were identified that were consistent across the lakes. The first pattern was one of monotonic change associated with changing abiotic lake conditions. The second was one of showing fluctuation patterns largely unrelated to gradual environmental change. Thus, two dominant and distinct temporal frequencies (temporal scales) were present in all lakes analysed. 4. Although the contribution of individual feeding groups varied between subarctic and hemiboreal lakes, they shared overall similar functional attributes (richness, evenness, diversity) and redundancies of functions within and between the observed temporal scales. This highlights similar resilience characteristics in subarctic and hemiboreal lakes. 5. Synthesis and applications. The effects of global change can be particularly strong at a single scale in ecosystems. Over time, this can cause monotonic change in communities and eventually lead to a loss of important ecosystem services upon reaching a critical threshold. Dynamics at other spatial or temporal scales can be unrelated to environmental change. The relative ‘intactness’ of these scales that are unaffected by global change and the persistence of functions at those scales may safeguard the whole system from the potential loss of functions at the scale at which global change impacts can be substantial. Thus, an understanding of scale-specific processes provides managers with a realistic assessment of vulnerabilities and the relative resilience of ecosystems to environmental change. Explicit consideration of ‘intact’ and ‘affected’ scales in analyses of global change impacts provides opportunities to tailor more specific management plans.

Journal of Applied Ecology↗

Effects of physical processes on structure and transport of thin zooplankton layers in the coastal ocean

Thin layers of plankton are recurrent features in a variety of coastal systems. These layers range in thickness from a few centimeters to a few meters. They can extend horizontally for kilometers and have been observed to persist for days. Densities of organisms found within thin layers are far greater than those above or below the layer, and as a result, thin layers may play an important role in the marine ecosystem. The paramount objective of this study was to understand the physical processes that govern the dynamics of thin layers of zooplankton in the coastal ocean. We deployed instruments to measure physical processes and zooplankton distribution in northern Monterey Bay; during an 11 d period of persistent upwelling-favorable winds, 7 thin zooplankton layers were observed. These zooplankton layers persisted throughout daylight hours, but were observed to dissipate during evening hours. These layers had an average vertical thickness of 1.01 m. No layers were found in regions where the Richardson number was <0.25. In general, when the Richardson number is <0.25 the water column is unstable, and incapable of supporting thin layers. Thin zooplankton layers were also located in regions of reduced flow. In addition, our observations show that the vertical depth distribution of thin zooplankton layers is modulated by high-frequency internal waves, with periods of 18 to 20 min. Results from this study clearly show an association between physical structure, physical processes and the presence of thin zooplankton layers in Monterey Bay. With this new understanding we may identify other coastal regions that have a high probability of supporting thin layers.

Marine Ecology Progress Series↗

Critical considerations for the application of environmental DNA methods to detect aquatic species

Species detection using environmental DNA (eDNA) has tremendous potential for contributing to the understanding of the ecology and conservation of aquatic species. Detecting species using eDNA methods, rather than directly sampling the organisms, can reduce impacts on sensitive species and increase the power of field surveys for rare and elusive species. The sensitivity of eDNA methods, however, requires a heightened awareness and attention to quality assurance and quality control protocols. Additionally, the interpretation of eDNA data demands careful consideration of multiple factors. As eDNA methods have grown in application, diverse approaches have been implemented to address these issues. With interest in eDNA continuing to expand, supportive guidelines for undertaking eDNA studies are greatly needed. Environmental DNA researchers from around the world have collaborated to produce this set of guidelines and considerations for implementing eDNA methods to detect aquatic macroorganisms. Critical considerations for study design include preventing contamination in the field and the laboratory, choosing appropriate sample analysis methods, validating assays, testing for sample inhibition and following minimum reporting guidelines. Critical considerations for inference include temporal and spatial processes, limits of correlation of eDNA with abundance, uncertainty of positive and negative results, and potential sources of allochthonous DNA. We present a synthesis of knowledge at this stage for application of this new and powerful detection method.

Methods in Ecology and Evolution↗