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Overview of issues in subsurface and landfill microbiology

To date, the majority of evidence indicates that most subsurface environments possess climax ecological communities that are well adapted to the environment in which they live. Like their counterparts on the surface, subsurface ecosystems are characterized by a high degree of microbiological diversity, they possess trophic structure, and they exhibit material cycling and energy transfer. Members of such ecosystems typically possess structural, physiological, or reproductive adaptations that allow them to disperse and survive in such habitats. Current studies argue that microbial activities influence the geochemical processes in both landfills and subsurface environments and that the altered geochemistry, in turn, influences the selection of microorganisms proliferating in the habitat. However, while microbial activity may often be limited by the availability of electron donors in uncontaminated aquifer systems, this is rarely the case with landfills. Sections in this chapter provide investigators with a greater understanding of the experimental approaches needed to study the microbiology of the terrestrial subsurface and an appreciation of interpretational limits imposed by the existing methodologies. The hope is that further study of subsurface microorganisms will provide insights into the process of microbial evolution and possibly into the origins of life itself.

Book chapter↗

Element concentrations and grain size of sediment from the Similkameen River above Enloe Dam (Enloe Reservoir) near Oroville, Washington, 2019

In 2019, the U.S. Geological Survey conducted a reconnaissance survey of concentrations of 41 trace elements present in bed sediment in the reservoir on the Similkameen River upstream from Enloe Dam, near Oroville, Washington. The Similkameen River drains a watershed containing highly mineralized geologic deposits with current (2019) and historical mining activity. Results of this survey indicated that surface and subsurface sediment are substantially enriched in element concentrations of silver (Ag), arsenic (As), gold (Au), bismuth (Bi), cadmium (Cd), copper (Cu), manganese (Mn), antimony (Sb), selenium (Se), tin (Sn), and tellurium (Te) relative to average concentrations found in upper continental-crustal material. Conversely, concentrations of mercury (Hg) and lead (Pb) in sediment above Enloe Dam (Enloe Reservoir) were generally less than average concentrations in upper continental-crustal material (Hg = 0.05 milligrams per kilogram [mg/kg]; Pb =17 mg/kg). Concentrations of most trace elements were higher in the less than 63-micrometer fraction (silt) and tended to be higher in subsurface than in surface sediment. The concentrations of trace elements were compared to consensus-based aquatic toxicity reference concentrations, Washington State Department of Ecology sediment management standards, and average concentrations of upper continental-crustal material. Arsenic concentrations were consistently elevated above these criteria among samples and often exceeded sediment management standards and aquatic toxicity reference values (both threshold effects and probable effects concentrations). High concentrations of As were measured in sediment with proportionally more material in the less than 63-micrometer size fraction; this result may be related to the presence of ore-processing waste material that has entered the aquatic system from approximately 125 years of mining operations in the basin. Elevated concentrations of chromium and copper that exceed the same criteria as arsenic (As) were measured less consistently and predominantly in the fine-grain size fraction.

Washington↗

Leaf litter decomposition and detrital communities following the removal of two large dams on the Elwha River (Washington, USA)

Large-scale dam removals provide opportunities to restore river function in the long-term and are massive disturbances to riverine ecosystems in the short-term. The removal of two dams on the Elwha River (WA, USA) between 2011 and 2014 was the largest dam removal project to be completed by that time and has since resulted in major changes to channel dynamics, river substrates, in-stream communities, and the size and shape of the river delta. To assess ecosystem function across the restored Elwha watershed, we compared leaf litter decomposition at twenty sites: 1) four tributary sites not influenced by restoration activities; 2) four river sites downstream of the upper dam (Glines Canyon Dam); 3) four river sites within the footprint of the former Aldwell Reservoir upstream of the lower dam (Elwha Dam); 4) four river sites downstream of the lower dam; and 5) four lentic sites in the newly developing Elwha delta. Three major findings emerged: 1) decomposition rates differed among sections of the Elwha watershed, with slowest decomposition rates at the delta sites and fastest decomposition rates just downstream of the upper dam; 2) aquatic macroinvertebrate communities establishing in leaf litterbags differed significantly among sections of the Elwha watershed; and 3) aquatic fungal communities growing on leaf litter differed significantly among sections. Aquatic macroinvertebrate and fungal diversity were sensitive to differences in canopy cover, water chemistry, and river bottom sediments across sites, with a stronger relationship to elevation for aquatic macroinvertebrates. As the Elwha River undergoes recovery following the massive sediment flows associated with dam removal, we expect to see changes in leaf litter processing dynamics and shifts in litter-dependent decomposer communities (both fungal and invertebrate) involved in this key ecosystem process.

Washington↗

High throughput computing: a solution for scientific analysis

Public land management agencies continually face resource management problems that are exacerbated by climate warming, land-use change, and other human activities. As the U.S. Geological Survey (USGS) Fort Collins Science Center (FORT) works with managers in U.S. Department of the Interior (DOI) agencies and other federal, state, and private entities, researchers are finding that the science needed to address these complex ecological questions across time and space produces substantial amounts of data. The additional data and the volume of computations needed to analyze it require expanded computing resources well beyond single- or even multiple-computer workstations. To meet this need for greater computational capacity, FORT investigated how to resolve the many computational shortfalls previously encountered when analyzing data for such projects. Our objectives included finding a solution that would: harness existing Computer Processing Units (CPUs) when they're idle to run multiple jobs concurrently, which reduces the overall processing time without requiring additional hardware; offer an effective, centralized job-management system; handle job failures due to hardware, software, or network interruptions (obviating the need to manually resubmit the job after each stoppage); be affordable; and most importantly, allow us to complete very large, complex analyses that otherwise would not even be possible. In short, we envisioned a job-management system that would take advantage of unused FORT CPUs within a local area network (LAN) to effectively distribute and run highly complex analytical processes. What we found was a solution that uses High Throughput Computing (HTC) and High Performance Computing (HPC) systems to do exactly that (Figure 1).

Report↗

A synopsis of short-term response to alternative restoration treatments in sagebrush-steppe: the SageSTEP project

The Sagebrush Steppe Treatment Evaluation Project (SageSTEP) is an integrated long-term study that evaluates ecological effects of alternative treatments designed to reduce woody fuels and to stimulate the herbaceous understory of sagebrush steppe communities of the Intermountain West. This synopsis summarizes results through 3 yr posttreatment. Woody vegetation reduction by prescribed fire, mechanical treatments, or herbicides initiated a cascade of effects, beginning with increased availability of nitrogen and soil water, followed by increased growth of herbaceous vegetation. Response of butterflies and magnitudes of runoff and erosion closely followed herbaceous vegetation recovery. Effects on shrubs, biological soil crust, tree cover, surface woody fuel loads, and sagebrush-obligate bird communities will take longer to be fully expressed. In the short term, cool wet sites were more resilient than warm dry sites, and resistance was mostly dependent on pretreatment herbaceous cover. At least 10 yr of posttreatment time will likely be necessary to determine outcomes for most sites. Mechanical treatments did not serve as surrogates for prescribed fire in how each influenced the fuel bed, the soil, erosion, and sage-obligate bird communities. Woody vegetation reduction by any means resulted in increased availability of soil water, higher herbaceous cover, and greater butterfly numbers. We identified several trade-offs (desirable outcomes for some variables, undesirable for others), involving most components of the study system. Trade-offs are inevitable when managing complex natural systems, and they underline the importance of asking questions about the whole system when developing management objectives. Substantial spatial and temporal heterogeneity in sagebrush steppe ecosystems emphasizes the point that there will rarely be a “recipe” for choosing management actions on any specific area. Use of a consistent evaluation process linked to monitoring may be the best chance managers have for arresting woodland expansion and cheatgrass invasion that may accelerate in a future warming climate.

Rangeland Ecology and Management↗

Luring anglers to enhance fisheries

Current fisheries management is, unfortunately, reactive rather than proactive to changes in fishery characteristics. Furthermore, anglers do not act independently on waterbodies, and thus, fisheries are complex socio-ecological systems . Proactive management of these complex systems necessitates an approach—adaptive fisheries management—that allows learning to occur simultaneously with management. A promising area for implementation of adaptive fisheries management is the study of luring anglers to or from specific waterbodies to meet management goals. Purposeful manipulation of anglers, and its associated field of study, is nonexistent in past management. Evaluation of different management practices (i.e., hypotheses) through an iterative adaptive management process should include both a biological and sociological survey to address changes in fish populations and changes in angler satisfaction related to changes in management. We believe adaptive management is ideal for development and assessment of management strategies targeted at angler participation. Moreover these concepts and understandings should be applicable to other natural resource users such as hunters and hikers.

Journal of Environmental Management↗

Permafrost mapping with electrical resistivity tomography in two wetland systems north of the Tanana River, Interior Alaska

Surface-based 2D electrical resistivity tomography (ERT) surveys were used to characterize permafrost distribution at wetland sites on the alluvial plain north of the Tanana River, 20 km southwest of Fairbanks, Alaska, in June and September 2014. The sites were part of an ecologically-sensitive research area characterizing biogeochemical response of this region to warming and permafrost thaw, and the site contained landscape features characteristic of interior Alaska, including thermokarst bog, forested permafrost plateau, and a rich fen. The results show how vegetation reflects shallow (0–10 m depth) permafrost distribution. Additionally, we saw shallow (0–3 m depth) low resistivity areas in forested permafrost plateau potentially indicating the presence of increased unfrozen water content as a precursor to ground instability and thaw. Time-lapse study from June to September suggested a depth of seasonal influence extending several meters below the active layer, potentially as a result of changes in unfrozen water content. A comparison of several electrode geometries (dipole-dipole, extended dipole-dipole, Wenner-Schlumberger) showed that for depths of interest to our study (0–10 m) results were similar, but data acquisition time with dipole-dipole was the shortest, making it our preferred geometry. The results show the utility of ERT surveys to characterize permafrost distribution at these sites, and how vegetation reflects shallow permafrost distribution. These results are valuable information for ecologically sensitive areas where ground-truthing can cause excessive disturbance. ERT data can be used to characterize the exact subsurface geometry of permafrost such that over time an understanding of changing permafrost conditions can be made in great detail. Characterizing the depth of thaw and thermal influence from the surface in these areas also provides important information as an indication of the depth to which carbon storage and microbially-mediated carbon processing may be affected.

Alaska↗

The Life of P: A biogeochemical and sociopolitical challenge in the Everglades

• Phosphorus (P) is an essential element for all life forms, yet to understand its life cycle and impact we need to grasp not only the biogeochemical life of P, but also how P intersects with human activities and values. • Phosphorus is the limiting nutrient in the oligotrophic Everglades ecosystem. Thus, the anthropogenic addition of P to the landscape and its subsequent transport, transformation, and persistence throughout the Everglades are critical to both the visual appearance and the ecological integrity of the Everglades ecosystem. • Legal and political attention to P in the Everglades have created a powerful social-cultural legacy. This legacy includes the continuing influence of P in Everglades restoration, the dissemination of knowledge about P cycling and mitigation among rural and urban Everglades residents, and social and political realignment of management priorities. • Management decisions based on legal mandates require not only robust scientific data about P-related processes, but also information on the behavior, well-being, and political decisions of tribal nations, agricultural enterprises, anglers, and others who use the South Florida landscape.

Florida↗

Optimization of salt marsh management at the Stewart B. McKinney National Wildlife Refuge, Connecticut, through use of structured decision making

Structured decision making is a systematic, transparent process for improving the quality of complex decisions by identifying measurable management objectives and feasible management actions; predicting the potential consequences of management actions relative to the stated objectives; and selecting a course of action that maximizes the total benefit achieved and balances tradeoffs among objectives. The U.S. Geological Survey, in cooperation with the U.S. Fish and Wildlife Service, applied an existing, regional framework for structured decision making to develop a prototype tool for optimizing tidal marsh management decisions at the Stewart B. McKinney National Wildlife Refuge in Connecticut. Refuge biologists, refuge managers, and research scientists identified multiple potential management actions to improve the ecological integrity of two marsh management units within the refuge and estimated the outcomes of each action in terms of performance metrics associated with each management objective. Value functions previously developed at the regional level were used to transform metric scores to a common utility scale, and utilities were summed to produce a single score representing the total management benefit that would be accrued from each potential management action. Constrained optimization was used to identify the set of management actions, one per marsh management unit, that would maximize total management benefits at different cost constraints at the refuge scale. Results indicated that, for the objectives and actions considered here, total management benefits may increase consistently up to approximately $ 1,190,000, but that further expenditures may yield diminishing return on investment. Management actions in optimal portfolios at total costs less than $1,190,000 included controlling avian predators in both management units, managing stormwater on lands adjacent to one marsh management unit, and removing a tide gate and breaching a dike to improve tidal flow in the other marsh management unit. The management benefits were derived from expected increases in the numbers of spiders (as an indicator of trophic health) and tidal marsh obligate birds, and an expected decrease in the use of herbicides to control invasive vegetation. The prototype presented here provides a framework for decision making at the Stewart B. McKinney National Wildlife Refuge that can be updated as new data and information become available. Insights from this process may also be useful to inform future habitat management planning at the refuges.

Connecticut↗

Ecological values of shallow-water habitats: Implications for the restoration of disturbed ecosystems

A presumed value of shallow-habitat enhanced pelagic productivity derives from the principle that in nutrient-rich aquatic systems phytoplankton growth rate is controlled by light availability, which varies inversely with habitat depth. We measured a set of biological indicators across the gradient of habitat depth within the Sacramento–San Joaquin River Delta (California) to test the hypothesis that plankton biomass, production, and pelagic energy flow also vary systematically with habitat depth. Results showed that phytoplankton biomass and production were only weakly related to phytoplankton growth rates whereas other processes (transport, consumption) were important controls. Distribution of the invasive clam Corbicula fluminea was patchy, and heavily colonized habitats all supported low phytoplankton biomass and production and functioned as food sinks. Surplus primary production in shallow, uncolonized habitats provided potential subsidies to neighboring recipient habitats. Zooplankton in deeper habitats, where grazing exceeded phytoplankton production, were likely supported by significant fluxes of phytoplankton biomass from connected donor habitats. Our results provide three important lessons for ecosystem science: (a) in the absence of process measurements, derived indices provide valuable information to improve our mechanistic understanding of ecosystem function and to benefit adaptive management strategies; (b) the benefits of some ecosystem functions are displaced by water movements, so the value of individual habitat types can only be revealed through a regional perspective that includes connectedness among habitats; and (c) invasive species can act as overriding controls of habitat function, adding to the uncertainty of management outcomes.

Ecosystems↗

A population model of the lizard Uta stansburiana, in southern Nevada

Population densities, reproduction, and survival of the lizard Uta stansburiana were measured at the Nevada Test Site in southern Nevada, USA, between 1964 and 1974. These data were used to develop a model of the population dynamics of this species. Results of irrigation experiments in 0.4—ha enclosures near Mercury, Nevada, were used to formulate multiple—regression equations predicting frequency and size of clutches laid by two age—classes of females in terms of winter rainfall, March air temperatures, and Uta population density. Densities of Uta in these enclosures were manipulated, and age—specific survival modeled in terms of spring densities of Uta. Experiments in which an important predator on Uta (the leopard lizard, Crotaphytus wislizeni) was removed from enclosures were used to estimate the influence of the predator on basic survival rates of hatchling and older Uta. The model was generally developed from data acquired in the small enclosures, but predictions were compared with actual observations of changes in Uta populations in Rock Valley (19 km west of Mercury, Nevada) between 1966 and 1972. Agreement between model predictions and actual numbers was fair. The model predicted a decrease in density from 1966 to 1967, but numbers of Uta actually increased conspicuously at this time. This was the only major discrepancy between predictions and observations. The observed mean spring density (d) between 1967 and 1972 was 41.4 Uta/ha (Sd = 20.8), while the model predicted a mean density of 37.8 Uta/ha (SD = 13.6). Observed and predicted mean proportions of yearlings in spring populations were identical (0.78). The basic version of the model estimated different survival rates for two age—groups of adult Uta. A simpler version of the model, using a common survival rate for both age—groups, gave predictions essentially identical with those of the basic model. Other tests of the basic model showed it to be most sensitive to changes in winter rainfall and predation pressure, much less so to air temperatures. Fifteen— and 30—yr synthetic sequences of predator densities were used to examine model stability over longer periods of time. When predator densities were drawn randomly from distributions with a mean of 2 individuals/ha, model populations exhibited lower mean numbers and amplitudes than actually observed during 9 yr in Rock Valley. The basic model included three density—dependent parameters: clutch frequency, clutch size, and adult survival. The model was modified so that (1) egg production was density independent, while adult survival was not; (2) adult survival was density independent, but egg production was not; and (3) there was no density dependence in the model. Thirty—year tests showed that cases 1 and 2 did not differ markedly from the basic model, although the removal of one density—dependent constraint resulted in slightly higher mean densities. In case 3, the model lacked stability and predicted numbers increased to unrealistic levels within 5 yr. We conclude that processes relating to egg production were modeled more effectively than those influencing survival, and that improvement of the model will depend on more detailed studies of the impact of predation on age—specific survival rates of Uta.

Nevada↗

Optimization of salt marsh management at the Long Island National Wildlife Refuge Complex, New York, through use of structured decision making

Structured decision making is a systematic, transparent process for improving the quality of complex decisions by identifying measurable management objectives and feasible management actions; predicting the potential consequences of management actions relative to the stated objectives; and selecting a course of action that maximizes the total benefit achieved and balances tradeoffs among objectives. The U.S. Geological Survey, in cooperation with the U.S. Fish and Wildlife Service, applied an existing, regional framework for structured decision making to develop a prototype tool for optimizing tidal marsh management decisions at the Long Island National Wildlife Refuge Complex in New York. Refuge biologists, refuge managers, and research scientists identified multiple potential management actions to improve the ecological integrity of five marsh management units within the refuge complex and estimated the outcomes of each action in terms of performance metrics associated with each management objective. Value functions previously developed at the regional level were used to transform metric scores to a common utility scale, and utilities were summed to produce a single score representing the total management benefit that could be accrued from each potential management action. Constrained optimization was used to identify the set of management actions, one per marsh management unit, that could maximize total management benefits at different cost constraints at the refuge-complex scale. Results indicated that, for the objectives and actions considered here, total management benefits may increase consistently up to about $24,000 , but that further expenditures may yield diminishing return on investment. Potential management actions in optimal portfolios at total costs less than $24,000 consistently included approaches for increasing drainage from the marsh surface within the marsh management units. The potential management benefits were derived from expected improvements in surface-water drainage and capacity for marsh elevation to keep pace with sea-level rise, and presumed increases in numbers of spiders (as an indicator of trophic health) and tidal marsh obligate birds. The prototype presented here does not resolve management decisions; rather, it provides a framework for decision making at the Long Island National Wildlife Refuge Complex that can be updated as new data and information become available. Insights from this process may also be useful to inform future habitat management planning at the refuges.

New York↗

Small creatures, big challenge: A review of current knowledge and assessment of research needs to inform water withdrawal management for the Duck River's freshwater mussel fauna

The Duck River is a remarkable national resource, boasting outstanding biodiversity (Ahlstedt et al. 2017). It contains 151 fish species, over 60 mussel species, and 22 snail species, many of which are federally listed (Etnier and Starnes 1993; Johnson et al. 2013; Ahlstedt et al. 2017; Womble and Rosenberger 2025a). The Duck River watershed is at a pivotal time in its management and ecological history due to significant growth in human population and economic activity. The consequent increase in surface water demand presents challenges for sustainable water resource management. The Tennessee Department of Environment and Conservation (TDEC) has permitted or proposed to permit eight water utilities to withdraw over 77 million gallons per day (mgd) from the Duck River, representing > 40% increase from previously permitted amounts (USFWS 2025). On November 20, 2024, Tennessee Governor Bill Lee signed Executive Order No 108 to ensure long-term stability of the Duck River watershed through balancing economic growth, water resource management, and environmental conservation. Some primary components of this plan are the creation of a Comprehensive Regional Drought Management Plan, the development of a Habitat Conservation Plan, and the establishment of the Duck River Watershed Planning Partnership (DRWPP). Although the planning partnership is not focused on a sole aspect of the Duck River, but the watershed as a whole, the mussel assemblages of the Duck River have been a particular focus due to their importance in providing ecosystem services, sensitivity to water quality, conservation status, and sensitivity to periodic drought conditions in this system. With multiple state and federal agencies involved in managing the Duck River’s unique water resources, a focused review on how future water management could affect the freshwater mussel communities in the Duck River will contribute to this process. Our objectives of this review are to: 1. Review the effects of changing flow regimes on mussels, with emphasis on the effect of droughts/low flows, highlighting key processes/factors affecting mussels. 2. Highlight critical data gaps that could allow future, evidence-based water management decisions in this system. 3. Propose research topics to address critical data gaps and enable evidence-based water management decisions that consider the needs of freshwater mussels. Freshwater mussels are a critical component of riverine ecosystems, providing ecosystem services including water filtration, nutrient cycling, and habitat provision (Vaughn et al. 2004; Haag 2012) that benefit human communities. Environmental flows, also referred to as ecological or e-flows, refer to the quantity, timing, and quality of water flows necessary to maintain the ecological health of rivers, wetlands, and other aquatic ecosystems (Acreman and Dunbar 2004; Acreman 2016). For freshwater mussels, environmental flows include maintaining a flow regime and associated water quality regimes that are favorable for both juveniles and adults while also seasonally supporting host fish and the survival of settling glochidia (i.e., larval mussels) in suitable environments (Gates et al. 2015). Freshwater mussels are adapted to the natural flow regime of their native streams, including seasonal variability in flows. As sedentary filter feeders, mussels require adequate water flow to provide oxygen and food (Allen and Vaughn 2009); however, they also require periods of low flow to increase access to fish hosts and to ensure juveniles are not displaced (Gates et al 2015). Mussels are sensitive to water quality conditions, including pollutants, sedimentation, and artificial alterations of temperature and pH (Strayer and Malcom 2012). Mussel community characteristics, including presence, abundance, and species richness, generally increase with stream size (e.g., stream order); however, responses to changes in flow conditions are often species-specific, and the magnitude and timing of extreme flow conditions have more predictive power for mussel community response than general changes in flow (Lopez and Vaughn 2021). Complex hydraulic variables, such as relative shear stress and Reynolds number, can be strong predictors of mussel community responses because they combine simple hydraulic variables (e.g., velocity, depth) with stream channel characteristics, such as substrate composition and dynamics. Droughts directly and indirectly affect mussels by influencing their physiology, behavior, reproduction, and ecosystem roles. Mass mussel die-offs during droughts and low water conditions are widely documented (Vaughn et al. 2015; Sousa et al. 2018; Dubose et al. 2019). Droughts can also lead to a shift in mussel community composition, with drought-tolerant species becoming more dominant while more sensitive species experience declines (Galbraith et al, 2010; Lopez et al. 2022; Vaughn and Atkinson 2025). Droughts affect mussels through direct mortality from stranding as well as physiological stress from altered water temperature and quality, reduced habitat and food availability and quality, increased predation and competition, disruption of reproductive cycles, and increased susceptibility to disease and pathogens. Although the effects of droughts, water withdrawals, and low flow conditions on mussels are an increasing area of study, there is a general paucity of data-driven consensus regarding optimal mitigation approaches and strategies (Cushway et al. 2024). Long-term management decisions regarding water withdrawals, particularly during extended or multi-year drought conditions, may consider factors beyond direct mortality such as the sublethal effects of drought and low flow conditions on freshwater mussels for a comprehensive understanding of the effects of prolonged low flows on freshwater mussels and aquatic communities.

Tennessee↗

Optimization of salt marsh management at the Rhode Island National Wildlife Refuge Complex through use of structured decision making

Structured decision making is a systematic, transparent process for improving the quality of complex decisions by identifying measurable management objectives and feasible management actions; predicting the potential consequences of management actions relative to the stated objectives; and selecting a course of action that maximizes the total benefit achieved and balances tradeoffs among objectives. The U.S. Geological Survey, in cooperation with the U.S. Fish and Wildlife Service, applied an existing, regional framework for structured decision making to develop a prototype tool for optimizing salt marsh management decisions at the Rhode Island National Wildlife Refuge Complex. Refuge biologists, refuge managers, and research scientists identified multiple potential management actions to improve the ecological integrity of nine salt marsh management units within the refuge complex and estimated the outcomes of each action in terms of performance metrics associated with each management objective. Value functions previously developed at the regional level were used to transform metric scores to a common utility scale, and utilities were summed to produce a single score representing the total management benefit that would be accrued from each potential management action. Constrained optimization was used to identify the set of management actions, one per salt marsh management unit, that would maximize total management benefits at different cost constraints at the refuge scale. Results indicated that, for the objectives and actions considered here, total management benefits may increase consistently up to approximately $ 150,000, but that further expenditures may yield diminishing return on investment. Management actions in optimal portfolios at total costs less than $ 150,000 included digging runnels (by hand or machine) on the marsh surface to improve drainage in eight management units, applying sediment to the marsh surface (thin layer deposition) in one management unit, constructing islands for use by tidal marsh obligate birds in two management units, and controlling Phragmites australis in one management unit. The management benefits were derived from expected improvements in the capacity for marsh elevation to keep pace with sea-level rise and increases in numbers of spiders (as an indicator of trophic health) and tidal marsh obligate birds. The prototype presented here provides a framework for decision making at the Rhode Island National Wildlife Refuge Complex that can be updated as new data and information become available. Insights from this process may also be useful to inform future habitat management planning at the refuge.

Rhode Island↗

Multi-year, spatially extensive, watershed-scale synoptic stream chemistry and water quality conditions for six permafrost-underlain Arctic watersheds

Repeated sampling of spatially distributed river chemistry can be used to assess the location, scale, and persistence of carbon and nutrient contributions to watershed exports. Here, we provide a comprehensive set of water chemistry measurements and ecohydrological metrics describing the biogeochemical conditions of permafrost-affected Arctic watersheds. These data were collected in watershed-wide synoptic campaigns in six stream networks across northern Alaska. Three watersheds are associated with the Arctic Long-Term Ecological Research site at Toolik Field Station (TFS), which were sampled seasonally each June and August from 2016 to 2018. Three watersheds were associated with the National Park Service (NPS) of Alaska and the U.S. Geological Survey (USGS) and were sampled annually from 2015 to 2019. Extensive water chemistry characterization included carbon species, dissolved nutrients, and major ions. The objective of the sampling designs and data acquisition was to characterize terrestrial–aquatic linkages and processing of material in stream networks. The data allow estimation of novel ecohydrological metrics that describe the dominant location, scale, and overall persistence of ecosystem processes in continuous permafrost. These metrics are (1) subcatchment leverage, (2) variance collapse, and (3) spatial persistence. Raw data are available at the National Park Service Integrated Resource Management Applications portal (O'Donnell et al., 2021, https://doi.org/10.5066/P9SBK2DZ ) and within the Environmental Data Initiative (Abbott, 2021, https://doi.org/10.6073/pasta/258a44fb9055163dd4dd4371b9dce945 ).

Alaska↗

Acidic Depositions: Effects on Wildlife and Habitats

The phenomenon of 'acid rain' is not new; it was recognized in the mid-1800s in industrialized Europe. In the 1960s a synthesis of information about acidification began in Europe, along with predictions of ecological effects. In the U.S. studies of acidification began in the 1920s. By the late 1970s research efforts in the U.S. and Canada were better coordinated and in 1980 a 10-year research program was undertaken through the National Acid Precipitation Assessment Plan (NAPAP) to determine the causes and consequences of acidic depositions. Much of the bedrock in the northeastern U.S. and Canada contains total alkalinity of <200 ?eq 1-1, thus, it lacks acid-neutralizing capacity. In the U.S. about 5% of the land area and in Canada about 43 % of the land area is sensitive to acidic depositions. Further, these areas receive >20 kg/ha/yr of wet sulphate depositions and are vulnerable to acidifying processes. Acidic depositions contribute directly to acidifying processes of soil and soil water. Soils must have sufficient acid-neutralizing capacity or acidity of soil will increase. Natural soil-forming processes that lead to acidification can be accelerated by acidic depositions. Long-term effects of acidification are predicted, which will reduce soil productivity mainly through reduced availability of nutrients and mobilization of toxic metals. Severe effects may lead to major alteration of soil chemistry, soil biota, and even loss of vegetation. Several species of earthworms and several other taxa of soil-inhabiting invertebrates, which are important food of many vertebrate wildlife species, are affected by low pH in soil. Loss of canopy in declining sugar maples results in loss of insects fed on by certain neotropical migrant bird species. No definitive studies categorically link atmospheric acidic depositions with direct or indirect effects on wild mammals. Researchers have concentrated on vegetative and aquatic effects. Circumstantial evidence suggests that effects are probable for certain species of aquatic-dependent mammals (water shrew, mink, and otter) and that these species are at risk from the loss of foods or contamination of these foods by metals, especially methylmercury. Continued acidification of terrestrial habitats, to the extent that earthworm populations are broadly reduced, might expose some fossorial mammalian species to risk because of decline in their major prey species. Acidic deposition affects primarily aquatic habitats of avian species by disrupting food webs (ecological effects) and increasing amounts of available heavy metals (mercury, aluminum, cadmium) in prey of avian species (toxicological effects). The ecological effects of acidifying wetlands are to reduce acid-intolerant prey (invertebrates) and to change prey quality from high-calcium bearing prey to low-calcium bearing prey. The toxicological effects are to increase contamination by heavy metals, especially methylated mercury, in foods of breeding waterbirds. The combination of these 2 types of effects results in potentially lower survival of adults and reduced production, growth, or survival of young of many bird species. Effects of acidification on herpteofauna and their habitats are mainly reproductive failure of susceptible species and reduced or metal-contaminated foods for both amphibians and reptiles.

Wildlife Society Technical Review↗

Chronic and episodic acidification of streams along the Appalachian Trail corridor, eastern United States

Acidic atmospheric deposition has adversely affected aquatic ecosystems globally. As emissions and deposition of sulfur (S) and nitrogen (N) have declined in recent decades across North America and Europe, ecosystem recovery is evident in many surface waters. However, persistent chronic and episodic acidification remain important concerns in vulnerable regions. We evaluated acidification in 269 headwater streams during 2010–2012 along the Appalachian Trail (AT) that transits several ecoregions and is located downwind of high levels of S and N emission sources. Discharge was estimated by matching sampled streams to those of a nearby gaged stream and assuming equivalent daily mean flow percentiles. Charge balance acid‐neutralizing capacity (ANC) values were adjusted to the 15th (Q15) and 85th flow percentiles (Q85) by applying the ANC/discharge slope among sample pairs collected at each stream. A site‐based approach was applied to streams sampled twice or more and a second regression‐based approach to streams sampled once to estimate episodic acidification magnitudes as the ANC difference from Q15 to Q85. Streams with ANC <0 μeq/L doubled from 16% to 32% as discharge increased from Q15 to Q85 according to the site‐based approach. The proportion of streams with ANC <0 μeq/L at low flow and high flow decreased from north to south. Base cation dilution explained the greatest amount of episodic acidification among streams and variation in sulfate (SO 4 2− ) concentrations was a secondary explanatory variable. Episodic SO 4 2− patterns varied geographically with dilution dominant in northern streams underlain by soils developed in glacial sediment and increased concentrations dominant in southern streams with older, highly weathered soils. Episodic acidification increased as low‐flow ANC increased, exceeding 90 μeq/L in 25% of streams. Episodic increases in ANC were the dominant pattern in streams with low‐flow ANC values <30 μeq/L. Chronic and episodic acidification remain an ecological concern among AT streams. The approach developed here could be applied to estimate the magnitude and extent of chronic and episodic acidification in other regions recovering from decreasing levels of atmospheric S and N deposition.

Connecticut, Georgia, Maine, Massachusetts, Maryla↗

Density dependence and weather drive dabbling duck spatiotemporal distributions and intercontinental migration

Understanding migratory waterfowl spatiotemporal distributions is important because, in addition to their economic and cultural value, wild waterfowl can be infectious reservoirs of highly pathogenic avian influenza virus (HPAIV). Waterfowl migration has been implicated in regional and intercontinental HPAIV dispersal, and predictive capabilities of where and when HPAIV may be introduced to susceptible spillover hosts would facilitate biosecurity and mitigation efforts. To develop forecasts for HPAIV dispersal, an improved understanding of how individual birds interact with their environment and move on a landscape scale is required. Using an agent-based modeling approach, we integrated individual-scale energetics, species-specific morphology and behavior, and landscape-scale weather and habitat data in a mechanistic stochastic framework to simulate Mallard ( Anas platyrhynchos ) and Northern Pintail ( Anas acuta ) annual migration across the Northern Hemisphere. Our model recreated biologically realistic migratory patterns using a first principles approach to waterfowl ecology, behavior, and physiology. Conducting a limited structural sensitivity analysis comparing reduced models to eBird Status and Trends in reference to the full model, we identified density dependence as the main factor influencing spring migration and breeding distributions, and wind as the main factor influencing fall migration and overwintering distributions. We show evidence of weather patterns in Northeast Asia causing significant intercontinental pintail migration to North America. By linking individual energetics to landscape-scale processes, we identify key drivers of waterfowl migration while developing a predictive model responsive to daily weather patterns. This model paves the way for future waterfowl migration research predicting HPAIV transmission, climate change impacts, and oil spill effects.

Avian Research↗