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Interaction of xenobiotics with estrogen receptors α and β and a putative plasma sex hormone-binding globulin from channel catfish ( Ictalurus punctatus )

Estrogens are important regulators of physiological functions. Although environmental contaminants (xenoestrogens) which interfere with estrogen signaling are of increasing concern, there is only limited information about their ability to interact with estrogen-binding proteins (SHBG) or receptors (ER). Recombinant ER?? and ?? were obtained after transient transfection of COS-7 cells with channel catfish ER cDNA. Plasma from adult female channel catfish was the source of SHBG. Tritiated estradiol ( 3H-E2) was used in standard radioligand-binding assays to characterize the binding properties of channel catfish SHBG (ccfSHBG) and to estimate the inhibition constants for various estrogenic compounds. Binding of 3H-E2 to ccfSHBG was saturable and of high affinity with a Kd (??SE) of 1.9??0.14nM and a Bmax of 14.3??2.4pmol/mg protein (n=3 assays). Additionally, ccfSHBG displayed binding specificity for androgens and estrogens. Endosulfan, 4-nonylphenol, and 4-octylphenol displaced 3H-E2 binding to ccfSHBG albeit only at very high concentrations, whereas dieldrin and atrazine showed little displacement activity even at the highest concentrations used. The synthetic estrogen ethynylestradiol had higher affinity than E2 for ccfSHBG. This finding differs from results with human and rainbow trout SHBG. The alkylphenolic compounds (4-octylphenol and 4-nonylphenol) displayed some ability to displace 3H-E2 binding from ER?? and ?? at high concentrations, but dieldrin and atrazine had little binding activity for both ER subtypes and endosulfan for ER??. The xenobiotics tested generally showed equivalent or greater affinity for ER?? than ER??, whereas natural estrogens had much greater affinity for ER?? than ER??. These observations suggest that results of studies using fish tissue ER extracts must be interpreted with caution, since both ER subtypes may be present, and that the binding of xenoestrogens to SHBG must be taken into account for proper assessment of endocrine disruption caused by environmental contaminants.

General and Comparative Endocrinology↗

The history of stream gaging in Ohio

Introduction Streams are a natural resource that can influence economic growth and the development of communities. They supply water for many uses, provide habitat for aquatic plants and animals, and sup-port recreational activities such as boat-ing and fishing. The amount of water (flow) in a stream — either too little or too much — can seriously affect these uses and human life. By using a method called stream gaging , information about the flow in a stream and the fluctuations in that flow can be obtained. n its simplest form, stream gaging may consist of measuring any number of the following stream characteristics: stage (or depth), cross-sectional area, velocity, and (or) flow (or discharge). When the depth and velocity are measured at several points across the stream channel so that the flow (or discharge ) is calculated, a streamflow measurement (or discharge measurement ) is made. By making streamflow measurements at the same site on a stream over a wide range of stages, a relation between stage and discharge can be developed. This relation can be used to provide information on the magnitude and fluctuations of flow on a stream. If long-term or continuous information is needed on a specific site, a stream-gaging station can be established. A stream-gaging station is a site where stage is monitored and recorded and streamflow measurements are made to provide a stage-discharge relation. Ever since the first documented streamflow measurement was made in Ohio — on the Sandusky River — stream-gaging information has been used to minimize the effects of floods and droughts, determine locations for water intakes and wastewater-treatment plants, and provide data for many other uses. Today, stream gaging is a routine activity in managing the State’s water resources.

Ohio↗

Assessing the vulnerability of public-supply wells to contamination: Floridan aquifer system near Tampa, Florida

This fact sheet highlights findings from the vulnerability study of a public-supply well in Temple Terrace, Florida, northeast of Tampa. The well selected for study typically produces water at the rate of 700 gallons per minute from the Upper Floridan aquifer. Water samples were collected at the public-supply well and at monitoring wells installed in or near the simulated zone of contribution to the supply well. Samples of untreated water from the public-supply wellhead contained the undesirable constituents nitrate, arsenic, uranium, radon-222, volatile organic compounds (VOCs), and pesticides, although all were detected at concentrations less than established drinking-water standards, where such standards exist. Overall, study findings point to four primary factors that affect the movement and fate of contaminants and the vulnerability of the public-supply well in Temple Terrace: (1) groundwater age (how long ago water entered, or recharged, the aquifer); (2) short-circuiting of contaminated water through sinkholes; (3) natural geochemical processes within the aquifer; and (4) pumping stress. Although the public-supply well is completed in the Upper Floridan aquifer, it produces water with concentrations of nitrate, VOCs, and the natural contaminant radon that are intermediate between the typical composition of water from the Upper Floridan aquifer and that of the overlying surficial aquifer system. Mixing calculations show that the water produced by the public-supply well could consist of upwards of 50 percent water from the surficial aquifer system mixed with water from the Upper Floridan aquifer. Anthropogenically affected water from the surficial aquifer system travels rapidly to depth through sinkholes that must be directly connected to the cavernous zone intersected by the public-supply well (and several other production wells in the region). Such solution features serve as fast pathways to the well and circumvent the natural attenuation of nitrate and radon that occurs when water from the surficial aquifer flows downward through the confining unit and then through the Upper Floridan aquifer matrix. Roughly 50 percent of the simulated flow to the public-supply well consists of water less than about 10 years old, thus making the well vulnerable to contamination from human activities. Sampling at various depths in the public-supply well during pumping and nonpumping conditions showed that water entering the well from the cavernous zone had much higher arsenic concentrations during pumping conditions (18.9 ug/L) than during nonpumping conditions (4.2 ug/L). This implies that movement of arsenic to the public-supply well from the cavernous zone is enhanced by pumping. One possible explanation is that pumping increases the movement of water with elevated dissolved oxygen content through the cavernous zone, which causes dissolution of arsenic associated with pyrite. All public-supply wells in the area may not have the same level of vulnerability as the well studied - many of the public-supply wells in the region have lower pumping rates and longer open intervals that may draw in a larger proportion of old water that predates anthropogenic influences. Determining the similarity of water produced by various public-supply wells in the region to that of the surficial aquifer system is one measure of well vulnerability that could be used to prioritize monitoring and land-use planning efforts to protect the most vulnerable wells.

Florida↗

Tampa Bay Integrated Science Pilot Study: Baseline mapping, land surface dynamics and predictive modeling, and hazards vulnerability studies

Tampa Bay and its environs have experienced phenomenal urban growth and significant changes in land cover and land-use practices over the past 50 years. This trend is expected to continue, with the impact of human activity broadening geographically and intensifying throughout the region. One of the immediate impacts of urban growth is the creation of additional impervious surfaces, which in turn, generate increased urban runoff that contributes to higher levels of nutrient loading in water bodies throughout the area. To better understand these and other anthropogenic affects on the ecology of the natural environment of the region, this component of the Tampa Bay Pilot Study took a broad basin-wide view. This regional view was intended to provide geographic and temporal context for the smaller intensely studied sample field site locations within the estuarine environment.

Open-File Report↗

Climax-Type Porphyry Molybdenum Deposits

Climax-type porphyry molybdenum deposits, as defined here, are extremely rare; thirteen deposits are known, all in western North America and ranging in age from Late Cretaceous to mainly Tertiary. They are consistently found in a postsubduction, extensional tectonic setting and are invariably associated with A-type granites that formed after peak activity of a magmatic cycle. The deposits consist of ore shells of quartz-molybdenite stockwork veins that lie above and surrounding the apices of cupola-like, highly evolved, calc-alkaline granite and subvolcanic rhyolite-porphyry bodies. These plutons are invariably enriched in fluorine (commonly >1 percent), rubidium (commonly >500 parts per million), and niobium-tantalum (Nb commonly >50 parts per million). The deposits are relatively high grade (typically 0.1-0.3 percent Mo) and may be very large (typically 100-1,000 million tons). Molybdenum, as MoS2, is the primary commodity in all known deposits. The effect on surface-water quality owing to natural influx of water or sediment from a Climax-type mineralized area can extend many kilometers downstream from the mineralized area. Waste piles composed of quartz-silica-pyrite altered rocks will likely produce acidic drainage waters. The potential exists for concentrations of fluorine or rare metals in surface water and groundwater to exceed recommended limits for human consumption near both mined and unmined Climax-type deposits.

Open-File Report↗

February 2012 workshop jumpstarts the Mekong Fish Monitoring Network

The Mekong River in Southeast Asia travels through a basin rich in natural resources. The river originates on the northern slope of the world's tallest mountains, the Himalaya Range, and then drops elevation quickly through steep mountain gorges, tumbling out of China into Myanmar (Burma) and the Lao People's Democratic Republic (Lao PDR). The precipitous terrain of Lao PDR and Thailand generates interest in the river and its tributaries for hydropower development. The terrain, soils, water, and climate make it one of the world's most biologically rich regions. The Mekong's bounty is again on display in the Mekong River Delta, where rice production has successfully been increased to high levels making Vietnam second only to Thailand as the world's largest rice exporters. At least 800 fish species contribute to the natural resource bounty of the Mekong River and are the basis for one of the world's most productive fisheries that provide the primary protein source to more than 50 million people. Against this backdrop of rich natural resources, the U.S. Geological Survey (USGS) is working with the consulting firm FISHBIO, colleagues from the international Delta Research and Global Observation Network (DRAGON) Institute, and a broad contingent of Southeast Asian representatives and partners from abroad to increase knowledge of the Mekong River fisheries and to develop the capacity of permanent residents to investigate and understand these fisheries resources. With the Lower Mekong Basin (LMB) region facing the likelihood of significant environmental changes as a result of both human activities and global climate change, enhancing environmental understanding is critical. To encourage cooperation among the LMB scientists and managers in the study of the Mekong River's fisheries, FISHBIO and the USGS, with generous support from the U.S. State Department, hosted a workshop in Phnom Penh, Cambodia, in February 2012. Workshop participants were from Lao PDR, Thailand, Cambodia, and Vietnam. Representatives from the governments, universities, nongovernmental organizations, and the Mekong River Commission discussed current and potential methods and mechanisms of the Mekong Fish Monitoring Network. The goals of the workshop were to determine if the Network and associated databases were of interest and value to the LMB nations, to determine if future fisheries monitoring data would be comparable among the nations, and to establish methods and an organizational structure for collaborating on future monitoring and research. The participants in this international workshop agreed that the Network would be useful but would require additional funding to secure their full participation. The USGS and FISHBIO are collaboratively seeking additional funding to expand research participation and projects in all four LMB nations. If the Network can facilitate cooperation among many fisheries researchers in the LMB, the basin would become a model of cooperative international fishery studies and would increase the understanding of a river basin rich in natural resources.

Phnom Penh↗

U.S. Geological Survey Ground-Water Resources Program, 2001

Ground water is among the Nation's most important natural resources. It provides drinking water to urban and rural communities, supports irrigation and industry, sustains the flow of streams and rivers, and maintains riparian and wetland ecosystems. In many areas of the Nation, the future sustainability of ground-water resources is at risk from over use and contamination. Because ground-water systems typically respond slowly to human actions and climate variability, a long-term perspective is needed to manage this valuable resource. The U.S. Geological Survey Ground-Water Resources Program provides regional evaluations, fundamental data, and predictive tools to help assure the sustainability of our Nation's ground-water resources.

Fact Sheet↗

Using landscape metrics to characterize towns along an urban-rural gradient

Context Urban-rural gradients are useful tools when examining the influence of human disturbances on ecological, social and coupled systems, yet the most commonly used gradient definitions are based on single broad measures such as housing density or percent forest cover that fail to capture landscape patterns important for conservation. Objectives We present an approach to defining urban–rural gradients that integrates multiple landscape pattern metrics related to ecosystem processes important for natural resources and wildlife sustainability. Methods We develop a set of land cover composition and configuration metrics and then use them as inputs to a cluster analysis process that, in addition to grouping towns with similar attributes, identifies exemplar towns for each group. We compare the outcome of the cluster-based urban-rural gradient typology to outcomes for four commonly-used rule-based typologies and discuss implications for resource management and conservation. Results The resulting cluster-based typology defines five town types (urban, suburban, exurban, rural, and agricultural) and notably identifies a bifurcation along the gradient distinguishing among rural forested and agricultural towns. Landscape patterns (e.g., core and islet forests) influence where individual towns fall along the gradient. Designations of town type differ substantially among the five different typologies, particularly along the middle of the gradient. Conclusions Understanding where a town occurs along the urban-rural gradient could aid local decision-makers in prioritizing and balancing between development and conservation scenarios. Variations in outcomes among the different urban-rural gradient typologies raise concerns that broad-measure classifications do not adequately account for important landscape patterns. We suggest future urban-rural gradient studies utilize more robust classification approaches.

Connecticut. Maine, Massachusetts, New Hampshire, ↗

Normative standards for land use in Vermont: Implications for biodiversity

The conversion of natural lands to developed uses poses a great threat to global terrestrial biodiversity. Natural resource managers, tasked with managing wildlife as a public trust, require techniques for predicting how much and where wildlife habitat is likely to be converted in the future. Here, we develop a methodology to estimate the “social carrying capacity for development” – SK d – for 251 towns across the state of Vermont, USA. SK d represents town residents’ minimum acceptable human population size and level of development within town boundaries. To estimate SK d across towns within the state of Vermont (USA), as well as the average state-wide SK d , we administered a visual preference survey ( n = 1505 responses) to Vermont residents, and asked respondents to rate alternative landuse scenarios in a fictional Vermont town on a scale of +4 (highly acceptable) to −4 (highly unacceptable). We additionally collected demographic data such as age and income, as well as ancillary information such as participation in town-planning meetings and location of residence. We used model selection and AIC to fit a cubic function to the response data, allowing us to estimate SK d at a town scale based on town demographic characteristics. On average, Vermonters had a SK d of 9.1% development on the landscape; this estimate is 68% higher than year 2000 levels for development (5.4%). Respondents indicated that management action to curb development was appropriate at 9.4% development (roughly the statewide SK d average). Management by local, regional, and state levels were considered acceptable for curbing development while federal level management of development was considered unacceptable. Given a scenario where development levels were at SK d , we predicted a 16,753 km 2 reduction in forested land (−11.16%) and a 1038 km 2 reduction in farmland (−60.45%). Such changes would dramatically alter biodiversity patterns state-wide. In a companion paper, we estimate how these changes would affect the distribution of wildlife species.

Vermont↗

Processes of wetland loss in India

Wetlands in India supply crucial human and animal needs such as drinking water, protein production, fodder, water purification, wildlife habitat, and flood storage. Increased appreciation of uses and threats is essential to protect wetlands where justified. Three quarters of India's population is rural, it places great demands on India's wetlands and losses continue to occur. This paper is based on extensive discussions with natural resource managers, government employees, farmers, academicians, and resource users at dozens of sites in India, as well as an extensive literature search. Twelve important kinds of wetland loss are identified and mechanisms believed to be causing them discussed: (1) agricultural conversion, (2) direct deforestation, (3) hydrologie alteration, (4) inundation, (5) defoliation, (6) altered upper watersheds, (7) accumulative water demands, (8) water quality degradation, (9) wetland consolidation, (10) global climate change, (11) ground-water depletion, (12) exotic species and biodiversity. Wetland understanding, management, and Public awareness in India must continue growing if wetland resources are to remain functional.

Environmental Conservation↗

Historic fire regime in southern California shrublands

Historical variability in fire regime is a conservative indicator of ecosystem sustainability, and thus understanding the natural role of fire in chaparral ecosystems is necessary for proper fire management. It has been suggested that the “natural” fire regime was one of frequent small fires that fragmented the landscape into a fine-grained mixture of age classes that precluded large, catastrophic fires. Some researchers claim that this regime was lost because of highly effective fire suppression and conclude that if fire managers could “restore” a regime of frequent fires with widespread prescription burning, they could eliminate the hazard of catastrophic fires. The primary evidence in support of this model is a study that compared contemporary burning patterns in southern California, U.S.A., a region subject to fire suppression, with patterns in northern Baja California, Mexico, where there is less effective fire suppression. We found that differences in fire regime between these two regions are inconclusive and could not be ascribed conclusively to differences in fire suppression. Historical records suggest that the natural fire regime in southern California shrublands was rather coarse-grained and not substantively different from the contemporary regime. There is no evidence that fire-management policies have created the contemporary fire regime dominated by massive Santa Ana wind-driven fires. Increased expenditures on fire suppression and increased loss of property and lives are the result of human demographic patterns that place increasing demand on fire-suppression forces.

Baja California, California↗

Klamath-Siskiyou herpetofauna: Biogeographic patterns and conservation strategies

The Klamath-Siskiyou region of southwest Oregon and northwest California (USA) has some of the most complex landscape mosaics and plant communities in western North America, reflecting its marked diversity of precipitation and topography. With 38 native species of amphibians and reptiles, the Klamath-Siskiyou region has the most species-rich herpetofauna of any similarly sized mountain range in the Pacific Northwest. Although it is a biodiversity 'hot spot,' there are only two endemic species, both salamanders, in the Klamath-Siskiyou region. High diversity is due to the overlap of two major biogeographic groups: the Arcto- (= northern) and Madro- (= southern) Tertiary herpetofaunas. Many of the amphibians in the Klamath-Siskiyou region are restricted to specialized habitats. Much of our knowledge about the biology of the regional fauna is based on studies elsewhere. Distributional surveys and ecological research are needed to address how the herpetofauna responds to timber harvest and other human activities that may reduce populations and increase fragmentation of suitable habitats. Conservation of the region's diverse herpetofauna should emphasize strategies directed at habitat specialists and species at the latitudinal limits of their ranges.

California, Oregon↗

Mute swans and their Chesapeake Bay habitats: proceedings of a symposium

The symposium 'Mute Swans and their Chesapeake Bay Habitats,' held on June 7, 2001, provided a forum for biologists and managers to share research findings and management ideas concerning the exotic and invasive mute swan (Cygnus olar). This species has been increasing in population size and is considered by many to be a problem in regard to natural food resources in the Bay that are used by native waterfowl during the winter months. Other persons, however, feel that resource managers are attempting to create a problem to justify more killing of waterfowl by hunters. Some persons also believe that managers should focus on the larger issues causing the decline of native food resources, such as the unabated human population increase in the Bay watershed and in the immediate coastal areas of the Bay. The symposium, sponsored by the Wildfowl Trust of North America and the U.S. Geological Survey, provided the atmosphere for presentation of mute swan data and opinions in a collegial setting where discussion was welcomed and was often informative and enthusiastic. An interesting historic review of the swan in regard to the history of mankind was presented, followed by a discussion on the positive and negative effects of invasive species. Biologists from different parts of the continent discussed the population status of the species in several states in the east and in the Great Lakes area. Data on the food habits of this species were presented in regard to submerged aquatic vegetation, and an interesting discussion on the role that the food habits of Canada geese in regard to native vegetation was presented. Findings and recommendations of the Mute Swan Task Force were presented. Finally, a representative of the Friends of Animals gave a thought-provoking presentation in defense of the mute swan. The presentations, in general, provided the necessary information and recommendations to allow managers to proceed with management of this controversial species with new and valuable perspectives.

Maryland, Virginia↗

Anthropogenic impacts to the recovery of the Mexican gray wolf with a focus on trapping-related incidents

Concerns regarding the potential negative impacts of regulated furbearer trapping to reintroduced Mexican gray wolves (Canis lupus baileyi), led to an executive order prohibiting trapping in the New Mexico, USA, portion of the Blue Range Wolf Recovery Area. This ban was to last for 6 months and required an evaluation of the risk posed to wolves by traps and snares legally permitted in New Mexico. We reviewed potential threats to wolves in the Blue Range Wolf Recovery Area, including threats associated with regulated furbearer trapping. One hundred Mexican gray wolf mortalities have been documented during the reintroduction effort (1998–2011). Of those mortalities with a known cause, >81% were human-caused resulting from illegal shooting (n = 43), vehicle collisions (n = 14), lethal removal by the United States Fish and Wildlife Service (USFWS; n = 12), non-project-related trapping (n = 2), project-related trapping (n = 1), and legal shooting by the public (n = 1). Ten wolves died due to unknown causes. The remaining 17 mortalities were a result of natural causes (e.g., starvation, disease). An additional 23 wolves were permanently, but non-lethally, removed from the wild by the USFWS. Of 13 trapping incidents in New Mexico that involved non-project trappers (i.e., trappers not associated with USFWS or U.S. Department of Agriculture-Wildlife Services), 7 incidents are known to have resulted in injuries to wolves: 2 wolves sustained injuries severe enough to result in leg amputations and 2 additional wolves died as a result of injuries sustained. Foothold traps with rubber-padded jaws and properly set snares may reduce trap-related injuries to Mexican gray wolves; however, impacts caused by trapping are overshadowed by other anthropogenic impacts (e.g., illegal shooting, non-lethal permanent removal, and vehicle collisions).

New Mexico↗

Global volcanic hazards and risk

An estimated 800 million people live within 100 km of an active volcano in 86 countries and additional overseas territories worldwide [see Chapter 4 and Appendix B]1. Volcanoes are compelling evidence that the Earth is a dynamic planet characterised by endless change and renewal. Humans have always found volcanic activity fascinating and have often chosen to live close to volcanoes, which commonly provide favourable environments for life. Volcanoes bring many benefits to society: eruptions fertilise soils; elevated topography provides good sites for infrastructure (e.g. telecommunications on elevated ground); water resources are commonly plentiful; volcano tourism can be lucrative; and volcanoes can acquire spiritual, aesthetic or religious significance. Some volcanoes are also associated with geothermal resources, making them a target for exploration and a potential energy resource. Much of the time volcanoes are not a threat because they erupt very infrequently or because communities have become resilient to frequently erupting volcanoes. However, there is an everpresent danger of a long-dormant volcano re-awakening or of volcanoes producing anomalously large or unexpected eruptions. Volcanic eruptions can cause loss of life and livelihoods in exposed communities, damage or disrupt critical infrastructure and add stress to already fragile environments. Their impacts can be both short-term, e.g. physical damage, and long-term, e.g. sustained or permanent displacement of populations. The risk from volcanic eruptions and their attendant hazards is often underestimated beyond areas within the immediate proximity of a volcano. For example, volcanic ash hazards can have effects hundreds of kilometres away from the vent and have an adverse impact on human and animal health, infrastructure, transport, agriculture and horticulture, the environment and economies. The products of volcanism and their impacts can extend beyond country borders, to be regional and even global in scale. Although known historical loss of life from volcanic eruptions (since 1600 AD about 280,000 fatalities are recorded, Auker et al. (2013)) is modest compared to other major natural hazards, volcanic eruptions can be catastrophic for exposed communities. In 1985 the town of Armero in Colombia was buried by lahars (volcanic mudflows) with more than 21,000 fatalities due to relatively small explosive eruptions at the summit of Nevado del Ruiz volcano that partially melted a glacier (Voight, 1990).

Book chapter↗

Predicting redox conditions in groundwater at a national scale using random forest classification

Redox conditions in groundwater may markedly affect the fate and transport of nutrients, volatile organic compounds, and trace metals, with significant implications for human health. While many local assessments of redox conditions have been made, the spatial variability of redox reaction rates makes the determination of redox conditions at regional or national scales problematic. In this study, redox conditions in groundwater were predicted for the contiguous United States using random forest classification by relating measured water quality data from over 30,000 wells to natural and anthropogenic factors. The model correctly predicted the oxic/suboxic classification for 78 and 79% of the samples in the out-of-bag and hold-out data sets, respectively. Variables describing geology, hydrology, soil properties, and hydrologic position were among the most important factors affecting the likelihood of oxic conditions in groundwater. Important model variables tended to relate to aquifer recharge, groundwater travel time, or prevalence of electron donors, which are key drivers of redox conditions in groundwater. Partial dependence plots suggested that the likelihood of oxic conditions in groundwater decreased sharply as streams were approached and gradually as the depth below the water table increased. The probability of oxic groundwater increased as base flow index values increased, likely due to the prevalence of well-drained soils and geologic materials in high base flow index areas. The likelihood of oxic conditions increased as topographic wetness index (TWI) values decreased. High topographic wetness index values occur in areas with a propensity for standing water and overland flow, conditions that limit the delivery of dissolved oxygen to groundwater by recharge; higher TWI values also tend to occur in discharge areas, which may contain groundwater with long travel times. A second model was developed to predict the probability of elevated manganese (Mn) concentrations in groundwater (i.e., ≥50 μg/L). The Mn model relied on many of the same variables as the oxic/suboxic model and may be used to identify areas where Mn-reducing conditions occur and where there is an increased risk to domestic water supplies due to high Mn concentrations. Model predictions of redox conditions in groundwater produced in this study may help identify regions of the country with elevated groundwater vulnerability and stream vulnerability to groundwater-derived contaminants.

contiguous United States↗

Metabolic capability and phylogenetic diversity of Mono Lake during a bloom of the eukaryotic phototroph Picocystis sp. strain ML

Algal blooms in lakes are often associated with anthropogenic eutrophication; however, they can occur without the human introduction of nutrients to a lake. A rare bloom of the alga Picocystis sp. strain ML occurred in the spring of 2016 at Mono Lake, a hyperalkaline lake in California, which was also at the apex of a multiyear-long drought. These conditions presented a unique sampling opportunity to investigate microbiological dynamics and potential metabolic function during an intense natural algal bloom. We conducted a comprehensive molecular analysis along a depth transect near the center of the lake from the surface to a depth of 25 m in June 2016. Across sampled depths, rRNA gene sequencing revealed that Picocystis -associated chloroplasts were found at 40 to 50% relative abundance, greater than values recorded previously. Despite high relative abundances of the photosynthetic oxygenic algal genus Picocystis , oxygen declined below detectable limits below a depth of 15 m, corresponding with an increase in microorganisms known to be anaerobic. In contrast to previously sampled years, both metagenomic and metatranscriptomic data suggested a depletion of anaerobic sulfate-reducing microorganisms throughout the lake's water column. Transcripts associated with photosystem I and II were expressed at both 2 m and 25 m, suggesting that limited oxygen production could occur at extremely low light levels at depth within the lake. Blooms of Picocystis appear to correspond with a loss of microbial activity such as sulfate reduction within Mono Lake, yet microorganisms may survive within the sediment to repopulate the lake water column as the bloom subsides.

California↗

Status and trend of the Kittlitz's Murrelet Brachyramphus brevirostris in Glacier Bay, Alaska

We conducted standardized surveys for marine birds in Glacier Bay in seven years between 1991 and 2008. From our most recent survey, a combination of line- and strip-transect methods completed in 2008, we estimated that 4981 (95% CI 1293–8670) Kittlitz’s Murrelets Brachyramphus brevirostris resided in Glacier Bay during the month of June, together with 12 195 (5607–18 783) Marbled Murrelets B. marmoratus . When counts were prorated to assign unidentified Brachyramphus murrelets to species, population estimates increased to 5641 Kittlitz’s Murrelets and 13 810 Marbled Murrelets. Our surveys of bird numbers in Glacier Bay between 1991 and 2008 revealed that Kittlitz’s Murrelet declined by ≥85% during this period. Trend analysis suggested a rate of decline between -10.7% and -14.4% per year. No direct human impacts (e.g., bycatch, oil pollution, vessel disturbance) in our study area could fully account for a decline of this magnitude. Widespread declines of Brachyramphus murrelets and Harbor Seals Phoca vitulina in the Gulf of Alaska during the 1980s-1990s suggest large-scale influences on these marine predators, perhaps related to climate-mediated cycles in food supply. Other natural factors that may impact Glacier Bay populations include predation by avian and terrestrial predators, widespread glacial retreat and its effect on nesting and foraging habitats, and competition for food with marine predators whose abundance in Glacier Bay has increased markedly in recent years (Humpback Whales Megaptera novaeangliae and Steller Sea Lions Eumetopias jubatus ).

Alaska↗