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Evaluating the effects of replacing septic systems with municipal sewers on groundwater quality in a densely developed coastal neighborhood, Falmouth, Massachusetts, 2016–19

Land disposal of sewage wastewater through septic systems and cesspools is a major cause of elevated concentrations of nitrogen in the shallow coastal aquifers of southern New England. The discharge of nitrogen from these sources at the coast is affecting the environmental health of coastal saltwater bodies. In response, local, State, and Federal agencies are considering expensive actions to mitigate these effects, including installing municipal sewer systems. To increase the understanding of the effects of municipal sewering on groundwater quality discharging to coastal surface waters, a network of multilevel monitoring wells was established in a densely developed coastal neighborhood on the Maravista peninsula, Falmouth, Massachusetts, which was undergoing conversion from onsite septic disposal to municipal sewering. The geohydrology of the study area on the peninsula is generally characterized as consisting of fine to coarse, well-sorted sands containing 2.9 to 9.3 meters of fresh groundwater and a flow system characterized by a groundwater divide slightly west of the center of the peninsula. The magnitude of hydraulic gradients at the water table is gently sloping, ranging from 0.000032 to 0.00059, and affected by daily and bimonthly tidal fluctuations from adjacent coastal ponds. On the western side of the divide, upgradient from Little Pond, average linear groundwater velocities and traveltimes along shallow flow paths, estimated from observed hydraulic gradients and estimated aquifer hydraulic conductivity and effective porosity, range from 0.076 to 0.094 meters per day and 7.8 to 9.7 years, respectively. The groundwater monitoring network consists of 14 profile sites on the peninsula that each include a multilevel sampler for water-quality data collection and a shallow monitoring well for groundwater-level measurements. The study area encompasses about 230 residences that transitioned from onsite septic disposal to municipal sewering between spring 2017 and summer 2019. An additional multilevel sampler that was in a residential coastal setting but not undergoing sewering also was sampled periodically as a reference site. Elevated nitrogen, as compared to typical uncontaminated, fresh groundwater in the Cape Cod aquifer, predominately as nitrate, was measured in 15 water-quality profiles at nitrate concentrations as great as 26.2 milligrams per liter as nitrogen ( n =749; mean and median values were 5.1 and 4.1 milligrams per liter as nitrogen, respectively). At all 14 profile sites and the reference profile site on a nearby peninsula, wastewater effects were denoted by increased nitrate, boron, and specific conductance, and by decreased pH and dissolved oxygen. The highest concentrations of nitrate typically occurred in the deepest one-half of the freshwater zone and in intervals of suboxic and oxic groundwater. Thickness-weighted mean and maximum nitrate concentrations, and total nitrate mass from four sampling rounds, provided a metric to evaluate expected changes at the 14 profile sites on the peninsula. Nitrate concentrations varied moderately by site between sampling rounds through both the presewering (June 2016 and April 2017) and transitional periods (April 2018 and June 2019). Nitrate concentrations greater than the U.S. Environmental Protection Agency maximum contaminant level for nitrate in drinking water (10 milligrams per liter as nitrogen), were detected at 9 of the 14 profile sites and at the reference site. The average of the mean thickness-weighted nitrate concentrations for the four full sampling rounds was greater than 5.0 milligrams per liter as nitrogen at 8 sites (7 profile sites and the reference site) and greater than 8 milligrams per liter as nitrogen at 3 profile sites. The total nitrate mass per square meter of land area at each profile site ranged from 1,830 to 36,800 milligrams per square meter. Nitrate mass flux, across a 500-meter-long section upgradient from Little Pond and covering about 15 percent of the total pond shoreline length, ranged from 124.3 to 192.6 kilograms per year for the four full sampling rounds under three groundwater-flow conditions. The expected improvements in groundwater quality in the freshwater zone should be characterized by decreases in concentrations of dissolved total and inorganic nitrogen and common ions such as boron, chloride, and fluoride. A statistical analysis using the Regional Kendall test for sampling points grouped in specific depth ranges confirmed that water-quality changes were statistically significant in at least one depth group during the 3-year sampling period (nitrate: −0.76 milligram per liter per year; specific conductance: −12.1 microsiemens per centimeter at 25 degrees Celsius per year; dissolved oxygen: 0.82 milligram per liter per year); however, the rate at which the water-quality improvements will result in decreases in nitrate mass loads to the coastal ponds primarily depends on groundwater traveltimes and the rate of flushing of wastewater constituents from the aquifer.

Massachusetts↗

Adaptive harvest management for the Svalbard population of Pink-Footed Geese: 2014 progress summary

This document describes progress to date on the development of an adaptive harvest-management strategy for maintaining the Svalbard population of pink-footed geese (Anser brachyrhynchus) near their agreed target level (60 thousand) by providing for sustainable harvests in Norway and Denmark. Specifically, this report provides an assessment of the most recent monitoring information and its implications for the harvest management strategy. The development of a passively adaptive harvest management strategy requires specification of four elements: (a) a set of alternative population models, describing the effects of harvest and other relevant environmental factors; (b) a set of probabilities describing the relative credibility of the alternative models, which are updated each year based on a comparison of model predictions and monitoring information; (c) a set of alternative harvest quotas, from which a 3-year quota is chosen; and (d) an objective function, by which alternative harvest strategies can be evaluated and an optimal strategy chosen. By combining varying hypotheses about survival and reproduction, a suite of nine models have been developed that represent a wide range of possibilities concerning the extent to which demographic rates are density dependent or independent, and the extent to which spring temperatures are important. Five of the models incorporate density-dependent mechanisms that would maintain the population near a carrying capacity (i.e., in the absence of harvest) of 65k – 129k depending on the specific model. The remaining four models are density independent and predict an exponentially growing population even with moderate levels of harvest. The most current set of monitoring information was used to update model weights for the period 1991 – 2013. Current model weights suggest little or no evidence for density-dependent survival and reproduction. These results suggest that the pink-footed goose population may have recently experienced a release from density-dependent mechanisms, corresponding to the period of most rapid growth in population size. There was equivocal evidence for the effect of May temperature days (number of days with temperatures above freezing: TempDays) on survival and on reproduction. During the summer of 2013 we computed an optimal harvest strategy for the 3-year period 2013 – 2015. The strategy suggested that the appropriate annual harvest quota is 15 thousand. The 1-year harvest strategy calculated to determine whether an emergency closure of the hunting season is required this year suggested an allowable harvest of 25.0 thousand; thus, a hunting-season closure is not warranted. If the harvest quota of 15 thousand were met in the coming hunting season, the next population count would be expected to be 71.0 thousand. If only the most recent 4-year mean harvest were realized (11.3 thousand), a population size of 74.8 thousand would be expected. Simulations suggest that it will take approximately seven years at current harvest levels to reduce population size to the goal of 60 thousand. However, it is possible that the extension of the forthcoming hunting season in Denmark could result in a total harvest approaching 15 thousand; in this case, simulations suggest it would only take about three years to reach the goal.

Svalbard↗

Ontogenetic shifts in mesohabitat use of young-of-year Rio Grande blue sucker in the Big Bend region of the Rio Grande

Alteration of flow regimes by anthropogenic activities is one of the primary environmental problems in riverine systems. Understanding how hydrologic conditions can affect ontogenetic habitat shifts of imperiled fishes is important in order to develop conservation and management strategies for each life-history stage. We examined relationships between the abundance of young-of-the-year (YOY) Rio Grande Blue Sucker and various abiotic variables in the Trans-Pecos region of the Rio Grande in Texas, USA. We used open N -mixture modeling to better understand the factors affecting ontogenetic habitat shifts of the imperiled aridland river fish. In addition, we examined differences in Rio Grande Blue Sucker total length among three mesohabitat types (pool, riffle, and run). The results of open N -mixture modeling suggested that as pool area increased, the abundance of YOY Rio Grande Blue Sucker increased. Total length of YOY Rio Grande Blue Sucker also significantly differed among the three mesohabitat types. The total lengths of YOY Rio Grande Blue Sucker in pool habitats were lower than in other mesohabitats, suggesting that YOY Rio Grande Blue Sucker undergo ontogenetic habitat shifts into greater current velocity habitats as they grow. The habitat associations we documented support the growing body of research emphasizing the importance of maintaining sufficient and appropriately timed flows to avoid prolonged low flows that limit habitat availability for native fish species during sensitive life stages in the Rio Grande and other aridland rivers.

Texas↗

Chapter 11: Management considerations

We conducted an ecoregional assessment of sagebrush ( Artemisia spp. ) ecosystems in the Wyoming Basins and surrounding regions (WBEA) to determine broad-scale species-environmental relationships. Our goal was to assess the potential influence from threats to the sagebrush ecosystem on associated wildlife through the use of spatially explicit occurrence and abundance models. These models were developed using information from field surveys conducted along gradients of vegetation productivity and human disturbance integrated with spatial datasets delineating land cover, topography, and human land use in the WBEA area. Our evaluation included all sagebrush-associated wildlife species across multiple taxa whose habitat requirements and distributions were appropriate for modeling and interpretation at the broad scales of this assessment. Dominant land uses were included in delineating the human footprint. Although overall levels of the cumulative human footprint were generally low across the WBEA area, oil and gas activities have decreased the amount of shrubland habitats and increased fragmentation within development regions over the last century. At the scale of this assessment, the influence of humans was primarily expressed as an indirect function through actions that altered or reduced available habitat. We identified 65 plant species of conservation concern; 28 of 40 vertebrate species associated with sagebrush were species of concern in at least one state. We modeled environmental relationships for 15 wildlife species from data collected from surveys conducted in 2005 and 2006 designed to sample multiple species and taxa along land cover and land use gradients across the WBEA area. Occurrence of 3 species was negatively influenced by human features; anthropogenic features were a positive influence for 3 species, 8 had a mixed response, and 1 had no measurable relationship. Sagebrush land cover, considered in all wildlife models, was important to most species but differed among species in the proportion of sagebrush required and at what spatial extent. For most species examined, the spatial extent at which sagebrush cover influenced the probability of occupancy was much larger than an individual’s home range size. Exotic plants were strongly associated with human features, particularly roads, which may function as linear vectors to facilitate spread of exotic plants across the WBEA area. We used coarse-grained spatial and thematic data because of the large spatial extent (350,000 km 2 ) of the WBEA area and the need for a consistent land cover map for the region. Distributions of species occurrence or abundance mapped in this assessment need to be corroborated with information on population demographics. In addition, our results should be interpreted relative to assumptions inherent in broad-scale ecoregional assessments. Our assessment provides managers with extensive and detailed maps of occurrence and abundance, allowing for status assessments of native species, diversity and richness, natural communities, and ecological systems present within the Wyoming Basins.

Wyoming↗

Transgenic zebrafish reveal tissue-specific differences in estrogen signaling in response to environmental water samples

Background: Environmental endocrine disruptors (EED) are exogenous chemicals that mimic endogenous hormones, such as estrogens. Previous studies using a zebrafish transgenic reporter demonstrated that the EEDs bisphenol A and genistein preferentially activate estrogen receptors (ER) in the larval heart compared to the liver. However, it was not known whether the transgenic zebrafish reporter was sensitive enough to detect estrogens from environmental samples, whether environmental estrogens would exhibit similar tissue-specific effects as BPA and genistein or why some compounds preferentially target receptors in the heart. Methods: We tested surface water samples using a transgenic zebrafish reporter with tandem estrogen response elements driving green fluorescent protein expression (5xERE:GFP). Reporter activation was colocalized with tissue-specific expression of estrogen receptor genes by RNA in situ hybridization. Results: Selective patterns of ER activation were observed in transgenic fish exposed to river water samples from the Mid-Atlantic United States, with several samples preferentially activating receptors in embryonic and larval heart valves. We discovered that tissue-specificity in ER activation is due to differences in the expression of estrogen receptor subtypes. ERα is expressed in developing heart valves but not in the liver, whereas ERβ2 has the opposite profile. Accordingly, subtype-specific ER agonists activate the reporter in either the heart valves or the liver. Conclusion: The use of 5xERE:GFP transgenic zebrafish has revealed an unexpected tissue-specific difference in the response to environmentally relevant estrogenic compounds. Exposure to estrogenic EEDs in utero is associated with adverse health effects, with the potentially unanticipated consequence of targeting developing heart valves.

New Jersey;Pennsylvania;Virginia;West Virginia↗

Assessment of PDMS-water partition coefficients: implications for passive environmental sampling of hydrophobic organic compounds

Solid-phase microextraction (SPME) has shown potential as an in situ passive-sampling technique in aquatic environments. The reliability of this method depends upon accurate determination of the partition coefficient between the fiber coating and water (K f ). For some hydrophobic organic compounds (HOCs), K f values spanning 4 orders of magnitude have been reported for polydimethylsiloxane (PDMS) and water. However, 24% of the published data examined in this review did not pass the criterion for negligible depletion, resulting in questionable K f values. The range in reported K f is reduced to just over 2 orders of magnitude for some polychlorinated biphenyls (PCBs) when these questionable values are removed. Other factors that could account for the range in reported K f , such as fiber-coating thickness and fiber manufacturer, were evaluated and found to be insignificant. In addition to accurate measurement of K f , an understanding of the impact of environmental variables, such as temperature and ionic strength, on partitioning is essential for application of laboratory-measured K f values to field samples. To date, few studies have measured K f for HOCs at conditions other than at 20 degrees or 25 degrees C in distilled water. The available data indicate measurable variations in K f at different temperatures and different ionic strengths. Therefore, if the appropriate environmental variables are not taken into account, significant error will be introduced into calculated aqueous concentrations using this passive sampling technique. A multiparameter linear solvation energy relationship (LSER) was developed to estimate log K f in distilled water at 25 degrees C based on published physicochemical parameters. This method provided a good correlation (R2 = 0.94) between measured and predicted log K f values for several compound classes. Thus, an LSER approach may offer a reliable means of predicting log K f for HOCs whose experimental log K f values are presently unavailable. Future research should focus on understanding the impact of environmental variables on K f . Obtaining the data needed for an LSER approach to estimate K f for all environmentally relevant HOCs would be beneficial to the application of SPME as a passive-sampling technique.

Environmental Science & Technology↗

Monitoring and verifying changes of organic carbon in soil

Changes in soil and vegetation management can impact strongly on the rates of carbon (C) accumulation and loss in soil, even over short periods of time. Detecting the effects of such changes in accumulation and loss rates on the amount of C stored in soil presents many challenges. Consideration of the temporal and spatial heterogeneity of soil properties, general environmental conditions, and management history is essential when designing methods for monitoring and projecting changes in soil C stocks. Several approaches and tools will be required to develop reliable estimates of changes in soil C at scales ranging from the individual experimental plot to whole regional and national inventories. In this paper we present an overview of soil properties and processes that must be considered. We classify the methods for determining soil C changes as direct or indirect. Direct methods include field and laboratory measurements of total C, various physical and chemical fractions, and C isotopes. A promising direct method is eddy covariance measurement of CO 2 fluxes. Indirect methods include simple and stratified accounting, use of environmental and topographic relationships, and modeling approaches. We present a conceptual plan for monitoring soil C changes at regional scales that can be readily implemented. Finally, we anticipate significant improvements in soil C monitoring with the advent of instruments capable of direct and precise measurements in the field as well as methods for interpreting and extrapolating spatial and temporal information.

Climatic Change↗

Evidence for density-dependent effects on body composition of a large omnivore in a changing Greater Yellowstone Ecosystem

Understanding the density-dependent processes that drive population demography in a changing world is critical in ecology, yet measuring performance–density relationships in long-lived mammalian species demands long-term data, limiting scientists' ability to observe such mechanisms. We tested performance–density relationships for an opportunistic omnivore, grizzly bears ( Ursus arctos , Linnaeus, 1758) in the Greater Yellowstone Ecosystem, with estimates of body composition (lean body mass and percent body fat) serving as indicators of individual performance over two decades (2000–2020) during which time pronounced environmental changes have occurred. Several high-calorie foods for grizzly bears have mostly declined in recent decades (e.g., whitebark pine [ Pinus albicaulis , Engelm, 1863]), while increasing human impacts from recreation, development, and long-term shifts in temperatures and precipitation are altering the ecosystem. We hypothesized that individual lean body mass declines as population density increases (H1), and that this effect would be more pronounced among growing individuals (H2). We also hypothesized that omnivory helps grizzly bears buffer energy intake from changing foods, with body fat levels being independent from population density and environmental changes (H3). Our analyses showed that individual lean body mass was negatively related to population density, particularly among growing-age females, supporting H1 and partially H2. In contrast, population density or sex had little effect on body fat levels and rate of accumulation, indicating that sufficient food resources were available on the landscape to accommodate successful use of shifting food sources, supporting H3. Our results offer important insights into ecological feedback mechanisms driving individual performances within a population undergoing demographic and ecosystem-level changes. However, synergistic effects of continued climate change and increased human impacts could lead to more extreme changes in food availability and affect observed population resilience mechanisms. Our findings underscore the importance of long-term studies in protected areas when investigating complex ecological relationships in an increasingly anthropogenic world.

Idaho, Montana, Wyoming↗

Linking dynamic habitat selection with wading bird foraging distributions across resource gradients

Species distribution models (SDM) link species occurrence with a suite of environmental predictors and provide an estimate of habitat quality when the variable set captures the biological requirements of the species. SDMs are inherently more complex when they include components of a species' ecology such as conspecific attraction and behavioral flexibility to exploit resources that vary across time and space. Wading birds are highly mobile, demonstrate flexible habitat selection, and respond quickly to changes in habitat quality; thus serving as important indicator species for wetland systems. We developed a spatio-temporal, multi-SDM framework using Great Egret ( Ardea alba ), White Ibis ( Eudocimus albus ), and Wood Stork ( Mycteria Americana ) distributions over a decadal gradient of environmental conditions to predict species-specific abundance across space and locations used on the landscape over time. In models of temporal dynamics, species demonstrated conditional preferences for resources based on resource levels linked to differing temporal scales. Wading bird abundance was highest when prey production from optimal periods of inundation was concentrated in shallow depths. Similar responses were observed in models predicting locations used over time, accounting for spatial autocorrelation. Species clustered in response to differing habitat conditions, indicating that social attraction can co-vary with foraging strategy, water-level changes, and habitat quality. This modeling framework can be applied to evaluate the multi-annual resource pulses occurring in real-time, climate change scenarios, or restorative hydrological regimes by tracking changing seasonal and annual distribution and abundance of high quality foraging patches.

PLoS ONE↗

Decision analysis to support development of the Glen Canyon Dam long-term experimental and management plan

The U.S. Geological Survey, in cooperation with the Bureau of Reclamation, National Park Service, and Argonne National Laboratory, completed a decision analysis to use in the evaluation of alternatives in the Environmental Impact Statement concerning the long-term management of water releases from Glen Canyon Dam and associated management activities. Two primary decision analysis methods, multicriteria decision analysis and the expected value of information, were used to evaluate the alternative strategies against the resource goals and to evaluate the influence of uncertainty. A total of 18 performance metrics associated with 8 out of 12 resource goals (fundamental objectives) were developed by the Bureau of Reclamation and National Park Service in partnership with subject-matter teams composed of Federal, State, tribal, and private experts. A total of 19 long-term strategies associated with 7 alternatives were developed by the Bureau of Reclamation, National Park Service, Argonne National Laboratory, U.S. Geological Survey, and Cooperating Agencies. The 19 long-term strategies were evaluated against the 18 performance metrics using a series of coupled simulation models, taking into account the effects of several important sources of uncertainty. A total of 27 Federal, State, tribal, and nongovernmental agencies were invited by the Assistant Secretary of Interior to participate in a swing-weighting exercise to understand the range of perspectives about how to place relative value on the resource goals and performance metrics; 14 of the 27 chose to participate. The results of the swing-weighting exercise were combined with the evaluation of the alternatives to complete a multicriteria decision analysis. The effects of uncertainty on the ranking of long-term strategies were evaluated through calculation of the value of information. The alternatives and their long-term strategies differed across performance metrics, producing unavoidable tradeoffs; thus, there was no long-term strategy that was dominated by another across all performance metrics. When the performance of each alternative was weighted across performance metrics, three alternatives (B, D, and G) were top-ranked depending on the set of weights proposed: Alternative B was favored by those stakeholders that placed a high value on hydropower; Alternative G was favored by those stakeholders that placed a high value on the restoration of natural processes, like beachbuilding and natural vegetation; and Alternative D was favored by the remaining stakeholders. Surprisingly, these rankings were not sensitive to the critical uncertainties that were evaluated; that is, the choice of a preferred long-term strategy was sensitive to the value-based judgment about how to place relative weight on the resource goals but was not sensitive to the uncertainties in the system dynamics that were evaluated in this analysis. The one area of uncertainty that did slightly affect the ranking of alternatives was the long-term pattern of hydrological input; because of this sensitivity, some attention to the possible effects of climate change is warranted. The results of the decision analysis are meant to serve as only one of many sources of information that can be used to evaluate the alternatives proposed in the Environmental Impact Statement. These results only focus on those resource goals for which quantitative performance metrics could be formulated and evaluated; there are other important aspects of the resource goals that also need to be considered. Not all the stakeholders who were invited to participate in the decision analysis chose to do so; thus, the Bureau of Reclamation, National Park Service, and U.S. Department of Interior may want to consider other input.

Arizona↗

Demographic responses to climate change in a threatened Arctic species

The Arctic is undergoing rapid and accelerating change in response to global warming, altering biodiversity patterns, and ecosystem function across the region. For Arctic endemic species, our understanding of the consequences of such change remains limited. Spectacled eiders ( Somateria fischeri ), a large Arctic sea duck, use remote regions in the Bering Sea, Arctic Russia, and Alaska throughout the annual cycle making it difficult to conduct comprehensive surveys or demographic studies. Listed as Threatened under the U.S. Endangered Species Act, understanding the species response to climate change is critical for effective conservation policy and planning. Here, we developed an integrated population model to describe spectacled eider population dynamics using capture–mark–recapture, breeding population survey, nest survey, and environmental data collected between 1992 and 2014. Our intent was to estimate abundance, population growth, and demographic rates, and quantify how changes in the environment influenced population dynamics. Abundance of spectacled eiders breeding in western Alaska has increased since listing in 1993 and responded more strongly to annual variation in first-year survival than adult survival or productivity. We found both adult survival and nest success were highest in years following intermediate sea ice conditions during the wintering period, and both demographic rates declined when sea ice conditions were above or below average. In recent years, sea ice extent has reached new record lows and has remained below average throughout the winter for multiple years in a row. Sea ice persistence is expected to further decline in the Bering Sea. Our results indicate spectacled eiders may be vulnerable to climate change and the increasingly variable sea ice conditions throughout their wintering range with potentially deleterious effects on population dynamics. Importantly, we identified that different demographic rates responded similarly to changes in sea ice conditions, emphasizing the need for integrated analyses to understand population dynamics.

Ecology and Evolution↗

Ontogeny of eDNA shedding during early development in Chinook Salmon (Oncorhynchus tshawytscha)

Knowledge of the timing of major life history events in aquatic species is important for informing conservation and resource management planning. Accordingly, surveys of environmental DNA (eDNA) have been performed to determine the efficacy of eDNA for providing information on life history events, primarily focusing on the timing of events associated with spawning, and these studies have proved successful. However, spawning represents only one part of the life history, and therefore, information on eDNA shedding during other life history stages is needed to fill gaps in knowledge. Here, we explored eDNA shedding during early life history (from fertilized eggs until near yolk sac absorption) in Chinook Salmon ( Oncorhynchus tshawytscha ) at three biomasses in a laboratory environment. We found that fertilized eggs shed little eDNA prior to hatching. Hatching coincided with a spike in eDNA, and we observed a significant and positive relationship between eDNA concentration and the number of hatched eggs. The concentration of eDNA shed by larvae after hatching was not consistent across post-hatch sampling days, suggesting developmental and behavioral changes associated with larval ontogeny may affect eDNA shedding rate. These results indicate that eDNA data may be used to identify hatch timing and verify successful reproduction in oviparous aquatic fishes. The application of eDNA to early life history broadens the capacity of eDNA-based methods for assessing population status and trends.

Environmental DNA↗

Dendrogeomorphic evidence of debris flow frequency and magnitude at Mount Shasta, California

Debris-flow deposits and woody vegetation adjacent to and growing within the channels of Whitney, Bolam, Mud, Ash, and Panthe creeks provide a 300-year record of debris-flow frequency at Mount Shasta Dendrochronologic (tree-ring) dating methods for the debris flows proved consistent with available documented records of debris flows Nine debris flows not reported in the historic record were documented and dated dendrochronologically. The oldest tree-ring date for a mudflow was about 1670 Combined geomorphic and botanical evidence shows that debris flows are a common occurrence at Mount Shasta Debris flows traveling at least 2 km have occurred at the rate of about 8 3 per century Smaller debris flows occur substantially more frequently and usually do not proceed as far downslope as larger debris flows. Cyclic scouring and filling by debris flows, in and adjacent to the stream channels, is suggested by dendrogeomorphic evidence and appears to be related to their magnitude and frequency Debris flows, small and large, may be the major surficial geomorphic agent in the vicinity of mount Shasta, sculpturing the channels and developing large alluvial fans ?? 1984 Springer-Verlag New York Inc.

California↗

Quantitative PCR detection of endangered diamond darter Crystallaria Cincotta in environmental DNA: Employing locked nucleic acids and blocking probe for specificity

This study presents a quantitative PCR (qPCR) assay for the detection of the endangered diamond darter Crystallaria cincotta from environmental DNA (eDNA) in water samples. The assay design is based on an alignment of mitochondrial cytochrome b DNA sequences from 58 individuals representing 25 percid species. Leveraging genetic differences, a species-specific qPCR assay was designed, incorporating alocked nucleic acid (LNA)-enriched probe and a secondary blocking probe to enhance specificity. The assay targets a 93-base pair fragment that includes a diagnostic single nucleotide polymorphism in the probe region; combined with multiple primer mismatches, this provides specificity for distinguishing C. cincotta from other sympatric percid species. Specificity was validated by testing genomic DNA from 16 percid species and synthetic templates, confirming no cross-reactivity. Performance metrics, including the standard curve, qPCR efficiency, limit of detection, and limit of quantification, are reported. The qPCR assay exhibited sufficient sensitivity to detect C. cincotta eDNA in environmental water samples collected from occupied riverine habitats. This study illustrates the effectiveness of LNA-enriched and blocking probes in developing species-specific qPCR assays for eDNA applications, demonstrating their utility in accurately distinguishing closely related species within diverse fish communities.

Conservation Genetics Resources↗

Limits to benthic feeding by eiders in a vital Arctic migration corridor due to localized prey and changing sea ice

Four species of threatened or declining eider ducks that nest in the Arctic migrate through the northeast Chukchi Sea, where anticipated industrial development may require prioritizing areas for conservation. In this nearshore corridor (10–40 m depth), the eiders’ access to benthic prey during the spring is restricted to variable areas of open water within sea ice. For the most abundant species, the king eider ( Somateria spectabilis ), stable isotopes in blood cells, muscle, and potential prey indicate that these eiders ate mainly bivalves when traversing this corridor. Bivalves there were much smaller than the same taxa in deeper areas of the northern Bering Sea, possibly due to higher mortality rates caused by ice scour in shallow water; future decrease in seasonal duration of fast ice may increase this effect. Computer simulations suggested that if these eiders forage for >15 h/day, they can feed profitably at bivalve densities >200 m −2 regardless of water depth or availability of ice for resting. Sampling in 2010–2012 showed that large areas of profitable prey densities occurred only in certain locations throughout the migration corridor. Satellite data in April–May over 13 years (2001–2013) indicated that access to major feeding areas through sea ice in different segments of the corridor can vary from 0% to 100% between months and years. In a warming and increasingly variable climate, unpredictability of access may be enhanced by greater effects of shifting winds on unconsolidated ice. Our results indicate the importance of having a range of potential feeding areas throughout the migration corridor to ensure prey availability in all years. Spatial planning of nearshore industrial development in the Arctic, including commercial shipping, pipeline construction, and the risk of released oil, should consider these effects of high environmental variability on the adequacy of habitats targeted for conservation.

Alaska↗

Nevada STORMS project: Measurement of mercury emissions from naturally enriched surfaces

Diffuse anthropogenic and naturally mercury-enriched areas represent long-lived sources of elemental mercury to the atmosphere. The Nevada Study and Tests of the Release of Mercury From Soils (STORMS) project focused on the measurement of mercury emissions from a naturally enriched area. During the project, concurrent measurements of mercury fluxes from naturally mercury-enriched substrate were made September 1–4, 1997, using four micrometeorological methods and seven field flux chambers. Ambient air mercury concentrations ranged from 2 to nearly 200 ng m −3 indicating that the field site is a source of atmospheric mercury. The mean daytime mercury fluxes, during conditions of no precipitation, measured with field chambers were 50 to 360 ng m −2 h −1 , and with the micrometeorological methods were 230 to 600 ng m −2 h −1 . This wide range in mercury emission rates reflects differences in method experimental designs and local source strengths. Mercury fluxes measured by many field chambers were significantly different ( p <0.05) but linearly correlated. This indicates that field chambers responded similarly to environmental conditions, but differences in experimental design and site heterogeneity had a significant influence on the magnitude of mercury fluxes. Data developed during the field study demonstrated that field flux chambers are ideal for assessment of the physicochemical processes driving mercury flux and development of an understanding of the magnitude of the influence of individual factors on flux. In general, mean mercury fluxes measured with micrometeorological methods during daytime periods were nearly 3 times higher than mean fluxes measured with field flux chambers. Micrometeorological methods allow for derivation of a representative mercury flux occurring from an unconstrained system and provide an assessment of the actual magnitude and variability of fluxes occurring from an area.

Journal of Geophysical Research D: Atmospheres↗

Controls on lake pelagic primary productivity: Formalizing the nutrient-color paradigm

Understanding controls on primary productivity is essential for describing ecosystems and their responses to environmental change. Lake primary production is strongly controlled by inputs of nutrients and colored dissolved organic matter. While past studies have developed mathematical models of this nutrient-color paradigm, broad empirical tests of these models are scarce. We used data from 58 diverse and globally distributed temperate lakes to test such a model and improve understanding and prediction of the controls on lake primary production. These lakes varied widely in size (0.02-2300 km2), pelagic gross primary production (20-8000 mg C m-2 d-1), and other characteristics. Across these diverse systems, and given relatively limited inputs, model predictions of primary production were highly correlated with observed values derived from high-frequency sensor data. Our analysis provides a model structure, including calibrated parameter estimates, that may be broadly useful for understanding current and future patterns in lake primary production.

JGR Biogeosciences↗

Environment, taxonomy, and socioeconomics predict non-imperilment in freshwater fishes

Freshwater fishes are among the most threatened taxa, yet conservation assessments remain incomplete for many species. Freshwater fishes provide essential ecosystem services such as food security, recreational opportunities, and cultural significance. Despite heavy alterations to freshwater ecosystems, the reasons for species’ sensitivity and resistance to imperilment are unclear. To address this need, we develop a machine learning framework to predict global imperilment status for 10,631 freshwater fish species using a comprehensive set of environmental, socioeconomic, and intrinsic species-level predictors. Using updated IUCN Red List data, we train and validate Random Forest classifiers to distinguish imperiled (Vulnerable, Endangered, Critically Endangered) from non-imperiled species. We examine the relative influence of 52 variables derived from 12 global sources describing extrinsic environmental and socioeconomic factors and intrinsic species-specific characteristics. Our models achieve higher accuracy for non-imperiled species (90.1%) compared to imperiled species (81.8%), reflecting the greater heterogeneity of threats and conditions driving imperilment. Across models, key predictors include habitat variables, taxonomic order, hydrological characteristics, and disturbance indicators, underscoring the interplay between ecology, geography, and human pressures. This integrative, reproducible approach demonstrates the utility of machine learning for guiding proactive conservation and provides a scalable framework for global biodiversity risk assessment.

Nature Communications↗