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At least 163 records · Page 9Linked to original sources
USGS2021

It’s complicated…environmental DNA as a predictor of trout and char abundance in streams

The potential to provide inferences about fish abundance from environmental (e)DNA samples has generated great interest. However, the accuracy of these abundance estimates is often low and variable across species and space. A plausible refinement is the use of common aquatic habitat monitoring data to account for attributes that influence eDNA dynamics. We therefore evaluated the relationships between eDNA concentration and abundance of bull trout ( Salvelinus confluentus ), westslope cutthroat trout ( Oncorhynchus clarkii lewisi ) and rainbow trout ( Oncorhynchus mykiss ) at 42 stream sites in the Intermountain West (USA and Canada) and tested whether accounting for site-specific habitat attributes improved the accuracy of fish abundance estimates. eDNA concentrations were positively associated with fish abundance, but these relationships varied by species and site, and there was still considerable variation unaccounted for. Random site-level differences explained much of this variation, but specific habitat attributes of those sites explained relatively small amounts of this variation. Our results underscore that either eDNA sampling or environmental characterization will require further refinement before eDNA can be used reliably to estimate fish abundance in streams.

Idaho, Montana, Oregon, Washington↗
USGS2006

A laboratory and field evaluation of a portable immunoassay test for triazine herbicides in environmental water samples

The usefulness and sensitivity, of a portable immunoassay test for the semiquantitative field screening of water samples was evaluated by means of laboratory and field studies. Laboratory results indicated that the tests were useful for the determination of atrazine concentrations of 0.1 to 1.5 μg/L. At a concentration of 1 μg/L, the relative standard deviation in the difference between the regression line and the actual result was about 40 percent. The immunoassay was less sensitive and produced similar errors for other triazine herbicides. After standardization, the test results were relatively insensitive to ionic content and variations in pH (range, 4 to 10), mildly sensitive to temperature changes, and quite sensitive to the timing of the final incubation step, variances in timing can be a significant source of error. Almost all of the immunoassays predicted a higher atrazine concentration in water samples when compared to results of gas chromatography. If these tests are used as a semiquantitative screening tool, this tendency for overprediction does not diminish the tests' usefulness. Generally, the tests seem to be a valuable method for screening water samples for triazine herbicides.

International Journal of Environmental Analytical ↗
USGS2021

Relative abundance of coyotes (Canis latrans) influences gray fox (Urocyon cinereoargenteus) occupancy across the eastern United States

Gray fox ( Urocyon cinereoargenteus (Schreber, 1775)) populations in portions of the eastern United States have experienced declines whose trajectories differ from those of other mesocarnivore populations. One hypothesis is that gray fox declines may result from interspecific interactions, particularly competition with abundant coyotes ( Canis latrans Say, 1823). Alternatively, gray foxes may respond negatively to increased urbanization and reduced forest cover. To evaluate these hypotheses, we used single-species occupancy models of camera trap data to test the effects of habitat covariates, such as the amount of urbanization and forest, on coyote and gray fox occupancy. Additionally, we test the effect of an index based on an N -mixture model of the number of coyotes at each camera trap site on gray fox occupancy. Results indicate that occupancy probabilities of coyote and gray fox relate positively to the amount of forest, but they provided no evidence urban cover impacts gray foxes. Additionally, gray fox occupancy was negatively related to the index of the number of coyotes at each site. Our models support the idea that interactions with coyotes impact gray fox occupancy across the eastern United States. These results illustrate how large-scale studies can relate mechanisms identified within specific landscapes to phenomena observed at larger scales.

Canadian Journal of Zoology↗
USGS1976

A qualitative evaluation of Landsat imagery of Australian rangelands

The capability of multidate, multispectral ERTS-1 imagery of three different rangeland areas within Australia was evaluated for its usefulness in preparing inventories of rangeland types, assessing on a broad scale range condition within these rangeland types, and assessing the response of rangelands to rainfall events over large areas. For the three divergent rangeland test areas, centered on Broken W, Alice Springs and Kalgoorlie, detailed interpretation of the imagery only partially satisfied the information requirements set. It was most useful in the Broken Hill area where fenceline contrasts in range condition were readily visible. At this and the other sites an overstorey of trees made interpretation difficult. Whilst the low resolution characteristics and the lack of stereoscopic coverage hindered interpretation it was felt that this type of imagery with its vast coverage, present low cost and potential for repeated sampling is a useful addition to conventional aerial photography for all rangeland types.

The Australian Rangeland Journal↗
USGS1986

Antibodies against canine parvovirus of wolves of Minnesota: A serologic study from 1975 through 1985

Serum samples (n = 137) from 47 wild wolves (Canis lupus; 21 pups and 26 adults) were evaluated from 1975 to 1985 for antibodies against canine parvovirus, using the hemagglutination inhibition (HI) test. In addition, several blood samples (n = 35) from 14 of these wolves (6 pups and 8 adults) were evaluated simultaneously for erythrocyte and leukocyte counts, and for hemoglobin and blood urea nitrogen concentrations. Sixty-nine (50%) of the serum samples (35 wolves) had HI titers of greater than or equal to 256, whereas 68 (50%) of the samples (16 wolves) had HI titers of less than or equal to 128. Significant differences in the geometric mean titers were not found between pups and adults or between males and females. Of the 47 wolves evaluated, 12 (25%) developed a greater than or equal to fourfold increase in antibody titers during the 11-year period, with 2 wolves developing serologic conversions in 1976. The data indicate that canine parvovirus may have begun infecting wolves before or at the same time that it began infecting the dog population in the United States.

Journal of the American Veterinary Medical Associa↗
USGS2006

Quantity-activity relationship of denitrifying bacteria and environmental scaling in streams of a forested watershed

The spatial variability of subreach denitrification rates in streams was evaluated with respect to controlling environmental conditions, molecular examination of denitrifying bacteria, and dimensional analysis. Denitrification activities ranged from 0 and 800 ng-N gsed-1 d-1 with large variations observed within short distances (<50 m) along stream reaches. A log-normal probability distribution described the range in denitrification activities and was used to define low (16% of the probability distributibn), medium (68%), and high (16%) denitrification potential groups. Denitrifying bacteria were quantified using a competitive polymerase chain reaction (cPCR) technique that amplified the nirK gene that encodes for nitrite reductase. Results showed a range of nirK quantities from 103 to 107 gene-copy-number gsed.-1 A nonparametric statistical test showed no significant difference in nirK quantifies among stream reaches, but revealed that samples with a high denitrification potential had significantly higher nirK quantities. Denitrification activity was positively correlated with nirK quantities with scatter in the data that can be attributed to varying environmental conditions along stream reaches. Dimensional analysis was used to evaluate denitrification activities according to environmental variables that describe fluid-flow properties, nitrate and organic material quantities, and dissolved oxygen flux. Buckingham's pi theorem was used to generate dimensionless groupings and field data were used to determine scaling parameters. The resulting expressions between dimensionless NO3- flux and dimensionless groupings of environmental variables showed consistent scaling, which indicates that the subreach variability in denitrification rates can be predicted by the controlling physical, chemical, and microbiological conditions. Copyright 2006 by the American Geophysical Union.

Journal of Geophysical Research G: Biogeosciences↗
USGS1991

Effect of hunting on annual survival of grey ducks in New Zealand

We used band recovery data from grey ducks ( Anas superciliosa ) banded in New Zealand between 1957 and 1974 to test 2 null hypotheses: (1) hunting mortality is completely additive to natural sources of mortality, and (2) hunting mortality is completely compensated by changes in natural mortality. We modeled annual survival as a function of survival in the absence of hunting and the probability of death from hunting. The complete compensation hypothesis was rejected, but we were unable to reject the completely additive hypothesis. There was no evidence of sex- or age-specificity for the relationship between kill rate and annual survival rate. We used simulated data to evaluate model performance. Parameter estimates were unbiased despite the inclusion of estimates that lay outside the bounds of the parameter space, although model-based variance estimates were consistently less than empirical variances. Our results imply that harvest-restrictions may be useful in effecting change in annual survival rates of grey ducks.

Journal of Wildlife Management↗
USGS1998

Preliminary evaluation of a simple in vitro test for the diagnosis of type C botulism in wild birds

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of type C botulinum toxin ( Clostridium botulinum ) in wild birds. This simple, antigen-capture ELISA utilizes polystyrene immunosticks as the solid substrate, chicken antitoxin (IgY) as the coating antibody, rabbit antitoxin as the primary antibody, and peroxidase-labeled goat-anti-rabbit as the secondary antibody. To evaluate the immunostick ELISA as a diagnostic test for avian botulism, known concentrations of toxin were added to heparinized blood collected from healthy birds and tested by both the ELISA and mouse bioassay. Also, blood samples from 236 bird carcasses submitted to the National Wildlife Health Center (NWHC) for cause of death determinations were tested by both procedures. Using ≤ 0.5 ml as the test volume for both procedures, the ELISA was less sensitive, detecting 0.25 ng/ml of toxin compared to 0.12 ng/ml for the mouse bioassay. Using the same volume of test sample for diagnostic submissions (≤ 0.5 ml), the ELISA was positive for 60% of the 149 clinically-diagnosed cases of botulism, whereas the mouse bioassay was positive for 79%. However, we demonstrated that with larger sample volumes (≥ 1.0 ml), the sensitivity of the ELISA may be equivalent or better than the mouse test due to the concentrating effect of the ELISA procedure. These preliminary results suggest that when adequate sample volumes are available, the immunostick ELISA can replace the mouse test for the diagnosis of botulism in wild birds.

Journal of Wildlife Diseases↗
USGS2018

Effects of leg flags on nest survival of four species of Arctic‐breeding shorebirds

Marking wild birds is an integral part of many field studies. However, if marks affect the vital rates or behavior of marked individuals, any conclusions reached by a study might be biased relative to the general population. Leg bands have rarely been found to have negative effects on birds and are frequently used to mark individuals. Leg flags, which are larger, heavier, and might produce more drag than bands, are commonly used on shorebirds and can help improve resighting rates. However, no one to date has assessed the possible effects of leg flags on the demographic performance of shorebirds. At seven sites in Arctic Alaska and western Canada, we marked individuals and monitored nest survival of four species of Arctic‐breeding shorebirds, including Semipalmated Sandpipers ( Calidris pusilla ), Western Sandpipers ( C. mauri ), Red‐necked Phalaropes ( Phalaropus lobatus ), and Red Phalaropes ( P. fulicarius ). We used a daily nest survival model in a Bayesian framework to test for effects of leg flags, relative to birds with only bands, on daily survival rates of 1952 nests. We found no evidence of a difference in nest survival between birds with flags and those with only bands. Our results suggest, therefore, that leg flags have little effect on the nest success of Arctic‐breeding sandpipers and phalaropes. Additional studies are needed, however, to evaluate the possible effects of flags on shorebirds that use other habitats and on survival rates of adults and chicks.

Journal of Field Ornithology↗
USGS2003

Efficacy of Hydrogen Peroxide in Controlling Mortality Associated with External Columnaris on Walleye and Channel Catfish Fingerlings

The efficacy of hydrogen peroxide in controlling mortality associated with external columnaris infections on walleye Sander vitreus (formerly Stizostedion vitreum) and channel catfish Ictalurus punctatus fingerlings was evaluated in two on-site trials. Microscopic examination of skin scrapes before treatment confirmed the presence of bacteria indicative of columnaris disease. In separate trials, walleyes (12 g) and channel catfish (2.3 g) were exposed to a total of three every-other-day hydrogen peroxide treatments of 0, 50, 75, or 100 mg/L for 60 min. Each treatment regimen was tested in triplicate, and each replicate contained 13 walleyes or 26 channel catfish. Hydrogen peroxide exposures of 50 mg/L for 60 min significantly increased walleye survival relative to that at other treatment concentrations, including the controls. Exposures of 50, 75, or 100 mg/L of hydrogen peroxide for 60 min significantly increased channel catfish survival in comparison with that of controls. These trials indicated that hydrogen peroxide treatments effectively reduce mortality in walleyes and channel catfish infected with external columnaris.

North American Journal of Aquaculture↗
USGS1998

Multi-level slug tests in highly permeable formations: 1. Modification of the Springer-Gelhar (SG) model

A multi-level slug test model and a method for the evaluation of vertical profiles of hydraulic conductivity in highly permeable formations are developed. A double-packer system is employed to estimate local hydraulic conductivity. Depending on the formation and double-packer system parameters, the water level recovery in the tested well can exhibit a monotonic or oscillatory response. To discern information on aquifer properties from artifacts introduced by the measurement system, the theory of flow in an aquifer and a double-packer system is developed. The mathematical model incorporates features of the Springer and Gelhar (1991) model and reduces to the Bouwer and Rice (1976) model in a special case. The model involves equations of momentum and mass conservation for the double-packer system with quasi-steady well-aquifer interaction equations. The method is uniformly applicable for both monotonic and oscillatory well responses and can produce profiles of hydraulic conductivity for the tested well. The criterion is given to determine the type of well response for given slug test conditions.A multi-level slug test model and a method for the evaluation of vertical profiles of hydraulic conductivity in highly permeable formations are developed. A double-packer system is employed to estimate local hydraulic conductivity. Depending on the formation and double-packer system parameters, the water level recovery in the tested well can exhibit a monotonic or oscillatory response. To discern information on aquifer properties from artifacts introduced by the measurement system, the theory of flow in an aquifer and a double-packer system is developed. The mathematical model incorporates features of the Springer and Gelhar (1991) model and reduces to the Bouwer and Rice (1976) model in a special case. The model involves equations of momentum and mass conservation for the double-packer system with quasi-steady well-aquifer interaction equations. The method is uniformly applicable for both monotonic and oscillatory well responses and can produce profiles of hydraulic conductivity for the tested well. The criterion is given to determine the type of well response for given slug test conditions.

Journal of Hydrology↗
USGS2010

Possible generational effects of habitat degradation on alligator reproduction

Population decline of the American alligator (Alligator mississippiensis) was observed in Lake Apopka in central Florida, USA, in the early 1980s. This decline was thought to result from adult mortality and nest failure caused by anthropogenic increases in sediment loads, nutrients, and contaminants. Reproductive impairment also was reported. Extensive restoration of marshes associated with Lake Apopka has been conducted, as well as some limited restoration measures on the lake. Monitoring by the Florida Fish and Wildlife Conservation Commission (FFWCC) has indicated that the adult alligator population began increasing in the early 1990s. We expected that the previously reported high proportion of complete nest failure (??0) during the 1980s may have decreased. We collected clutches from alligator nests in Lake Apopka from 1983 to 2003 and from 5 reference areas from 1988 to 1991, and we artificially incubated them. We used a Bayesian framework with Gibbs sampler of Markov chain Monte Carlo simulation to analyze ??0. Estimated ??0was consistently higher in Lake Apopka compared with reference areas, and the difference in ??0 ranged from 0.19 to 0.56. We conducted change point analysis to identify and test the significance of the change point in ??0in Lake Apopka between 1983 and 2003, indicating the point of reproductive recovery. The estimated Bayes factor strongly supported the single change point hypothesis against the no change point hypothesis. The major downward shift in ??0 probably occurred in the mid-1990s, approximately a generation after the major population decline in the 1980s. Furthermore, estimated ??0 values after the change point (0.21) were comparable with those of reference areas (0.07-0.31). These results combined with the monitoring by FFWCC seem to suggest that anthropogenic habitat degradation caused reproductive impairment of adult females and decreases in ??0 occurred with the sexual maturity of a new generation of breeding females. Long-term monitoring is essential to understand population changes due to habitat restoration. Such information can be used as an input in planning and evaluating restoration activities.

Journal of Wildlife Management↗
USGS2019

A statewide evaluation of Florida Bass genetic introgression in Tennessee

Largemouth Bass (Micropterus salmoides) are one of the most popular freshwater sport fish in the United States and managers in southeastern states have stocked the Florida Bass (M. s. floridanus) subspecies outside of its natural range to increase size structure of existing Largemouth Bass populations. In Tennessee, fisheries for Largemouth Bass are concentrated in reservoirs of the Cumberland and Tennessee River systems; however, Florida Bass stockings have to date been restricted to two reservoirs of the Tennessee River and have varied significantly in extent and duration. We quantified levels of genetic admixture between Florida Bass and Largemouth Bass using 38 species-diagnostic single nucleotide polymorphisms for 979 fish sampled from 14 reservoirs from the Cumberland and Tennessee River systems. We tested for differences in genetic admixture across river systems and evaluated the relative importance of fish stockings in explaining observed levels of genetic admixture. Levels of genetic admixture and associated variation was higher in reservoirs of the Tennessee River (range = 6.3 – 30.4 average percent Florida Bass alleles) relative to the Cumberland River (range = 5.7 – 13.4 %), but admixture was not solely related to stocking rates. The highest levels of Florida Bass introgression were detected in Chickamauga Reservoir, the most extensively stocked reservoir in the state. Linear models identified fish stocked as a significant overall predictor of admixture, but we also observed stocked and unstocked reservoirs with similar levels of admixture, suggesting factors other than stocking influence population genetics. Our statewide assessment of reservoir-level patterns of hybridization among black bass was performed prior to the recent expansion of Florida Bass stocking efforts in Tennessee, and thus, represents a baseline for evaluating future stocking.

Tennessee↗
USGS2020

Relating hydroclimatic change to streamflow, baseflow, and hydrologic partitioning in the Upper Rio Grande Basin, 1980 to 2015

Understanding how changing climatic conditions affect streamflow volume and timing is critical for effective water management. In the Rio Grande Basin of the southwest U.S., decreasing snowpack, increasing minimum temperatures, and decreasing streamflow have been observed in recent decades, but the effects of hydroclimatic changes on baseflow, or groundwater discharge to streams, have not been investigated. In this study, we determine how trends in precipitation, snowpack accumulation, and snowmelt rate relate to total streamflow, baseflow, and the hydrologic partitioning of baseflow and runoff at 12 sites in the Upper Rio Grande Basin (URGB) during 1980 to 2015. Total streamflow was partitioned into baseflow and runoff components at a daily time step using conductivity-mass-balance hydrograph separation. Trends in annual total streamflow, baseflow, runoff, baseflow index, precipitation, snowmelt rate, and peak snow water equivalent (SWE) were evaluated from 1980 to 2015 using the non-parametric Mann-Kendall trend test. Results indicate that baseflow forms a large component of total streamflow, contributing an average of 49% of total discharge upstream of Albuquerque, NM. During 1980 to 2015, decreasing trends in total streamflow occurred at 9 of 12 sites and were almost always associated with decreases in baseflow, suggesting that baseflow volumes can respond to changing climatic and anthropogenic conditions within decades. Decreasing snowmelt rates were more frequently associated with decreases in baseflow and total streamflow than were decreases in precipitation and peak SWE, highlighting the importance of snowmelt rate as a process controlling streamflow generation. If snow accumulation and snowmelt rates continue to decrease in the future, results indicate that total streamflow and baseflow volumes will decline, and that baseflow will become a larger fraction of total streamflow in the URGB.

Colorado, New Mexico↗
USGS2004

Landscape scale measures of steelhead (Oncorhynchus mykiss) bioenergetic growth rate potential in Lake Michigan and comparison with angler catch rates

The relative quality of a habitat can influence fish consumption, growth, mortality, and production. In order to quantify habitat quality, several authors have combined bioenergetic and foraging models to generate spatially explicit estimates of fish growth rate potential (GRP). However, the capacity of GRP to reflect the spatial distributions of fishes over large areas has not been fully evaluated. We generated landscape scale estimates of steelhead ( Oncorhynchus mykiss ) GRP throughout Lake Michigan for 1994-1996, and used these estimates to test the hypotheses that GRP is a good predictor of spatial patterns of steelhead catch rates. We used surface temperatures (measured with AVHRR satellite imagery) and acoustically measured steelhead prey densities (alewife, Alosa pseudoharengus ) as inputs for the GRP model. Our analyses demonstrate that potential steelhead growth rates in Lake Michigan are highly variable in both space and time. Steelhead GRP tended to increase with latitude, and mean GRP was much higher during September 1995, compared to 1994 and 1996. In addition, our study suggests that landscape scale measures of GRP are not good predictors of steelhead catch rates throughout Lake Michigan, but may provide an index of interannual variation in system-wide habitat quality.

Lake Michigan↗
USGS2000

Efficacy of a type C botulism vaccine in green-winged teal

We tested the efficacy of a single dose of Botumink toxoid for protecting wild green-winged teal (Anas crecca) during botulism epizootics caused by Clostridium botulinum type C. We challenged control and immunized ducks with four different doses of type C botulinum toxin to determine the LD50 for this species and to evaluate vaccine protection. Fewer immunized ducks were affected with botulism than control ducks, indicating that a single dose of Botumink toxoid could increase the survival of ducks during epizootics. However, the frequency of immunized ducks with signs of botulism increased with the challenge dose of botulinum toxin. Even at doses of botulinum toxin approximately 2 to 4 green-winged teal LD50, about 50% of the immunized ducks were affected. We believe an improved vaccine or a better delivery system is required to justify immunization of wild birds for experimental survival studies.

California, Utah↗
USGS2026

Toxoplasma gondii: Challenges and perspectives in interpreting longitudinal seroprevalence data for a chronic parasitic infection

Toxoplasma gondii —the causative agent of toxoplasmosis—is a zoonotic pathogen of warm-blooded hosts. Infection causes mild-to-severe symptoms, including lethargy, fever, muscle pain, abortion, ocular disease, and encephalitis. Toxoplasma affects many vertebrate species, although felids are the only known definitive hosts. Seroprevalence in wildlife is often assessed using cross-sectional data, but few studies have tracked individual-level infections through time. We present a 4-yr dataset from white-tailed deer ( Odocoileus virginianus ) with repeated sampling of individuals that highlights challenges associated with assigning serostatus to individuals. Using a modified agglutination test, we observed seroconversion from seronegative to seropositive within individuals, as expected. Although toxoplasmosis is known to be a chronic disease, we also found reversion from seropositive to seronegative. Accurate assignment of serostatus is necessary for evaluating effects of infection on behavioral and physiologic outcomes. However, longitudinal data from individuals whose titers oscillate around the positive threshold present novel challenges. Therefore, we discuss the implications for assigning serostatus for chronic toxoplasmosis infection for three proposed approaches: 1) ever positive, always positive; 2) negative until positive and then always positive; and 3) status by sampling period. Clarifying which approach is used to assign serostatus when analyzing longitudinal T. gondii data may enable more meaningful comparisons across systems and studies.

Journal of Wildlife Diseases↗
USGS2017

No flood effect on recruitment of a small Louisiana black bear population

A flood event in 2011 had minor impacts on apparent survival and movement probabilities of a small, isolated population of Louisiana black bears ( Ursus americanus luteolus ) in the Upper Atchafalaya River Basin, Louisiana, USA. However, the potential effects of the flood on recruitment of juveniles into the population, then listed as threatened under the United States Endangered Species Act, were not evaluated. We used hair trapping data collected from 2007 to 2015 and Pradel temporal symmetry models in a robust‐design framework to investigate changes in per capita recruitment that could have resulted from the flood. We detected 91 bears (37 M:54 F) within the flooded area during our study period, ranging from 21 to 44 individuals/year. Models that tested for reduced recruitment resulting from the flood were not supported more than models with constant recruitment, and the population growth rate did not decline. Although we documented marginally lower recruitment following the 2011 flood, lag effects and detectability biases complicated our analysis. We suggest that wildlife managers continue monitoring recruitment and survival in this recently delisted black bear population given the potential for heightened flood frequency and severity in the future.

Journal of Wildlife Management↗