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Fort Collins Science Center: Fiscal Year 2007 Accomplishments

In Fiscal Year 2007 (FY07), the U.S. Geological Survey (USGS) Fort Collins Science Center (FORT) continued research vital to U.S. Department of the Interior science and management needs and associated USGS programmatic goals. FORT work also supported the science needs of other government agencies as well as private cooperators. Specifically, FORT scientific research and technical assistance focused on client and partner needs and goals in the areas of biological information management, fisheries and aquatic systems, invasive species, status and trends of biological resources, terrestrial ecosystems, and wildlife resources. In addition, FORT's 5-year strategic plan was refined to incorporate focus areas identified in the USGS strategic science plan, including ecosystem-landscape analysis, global climate change, and energy and mineral resource development. As a consequence, several science projects initiated in FY07 were either entirely new research dor amplifications of existing work. Highlights of FORT project accomplishments are described below under the USGS science program with which each task is most closely associated. The work of FORT's 6 branches (Aquatic Systems and Technology Applications, Ecosystem Dynamics, Information Science, Invasive Species Science, Policy Analysis and Science Assistance, and Species and Habitats of Federal Interest) often involves major partnerships with other agencies or cooperation with other USGS disciplines (Geology, Geography, Water Resources) and the Geospatial Information Office.

Open-File Report

Invasive Species Science Branch: research and management tools for controlling invasive species

Invasive, nonnative species of plants, animals, and disease organisms adversely affect the ecosystems they enter. Like “biological wildfires,” they can quickly spread and affect nearly all terrestrial and aquatic ecosystems. Invasive species have become one of the greatest environmental challenges of the 21st century in economic, environmental, and human health costs, with an estimated effect in the United States of more than $120 billion per year. Managers of the Department of the Interior and other public and private lands often rank invasive species as their top resource management problem. The Invasive Species Science Branch of the Fort Collins Science Center provides research and technical assistance relating to management concerns for invasive species, including understanding how these species are introduced, identifying areas vulnerable to invasion, forecasting invasions, and developing control methods. To disseminate this information, branch scientists are developing platforms to share invasive species information with DOI cooperators, other agency partners, and the public. From these and other data, branch scientists are constructing models to understand and predict invasive species distributions for more effective management. The branch also has extensive herpetological and population biology expertise that is applied to harmful reptile invaders such as the Brown Treesnake on Guam and Burmese Python in Florida.

Fact Sheet

Spatial and temporal relationships between the invasive snail Bithynia tentaculata and submersed aquatic vegetation in Pool 8 of the Upper Mississippi River

Bithynia tentaculata is an invasive snail that was first reported in Lake Michigan in 1871 and has since spread throughout a number of freshwater systems of the USA. This invasion has been extremely problematic in the Upper Mississippi River as the snails serve as intermediate hosts for several trematode parasites that have been associated with waterfowl mortality in the region. This study was designed to assess the abundance and distribution of B . tentaculata relative to submersed aquatic vegetation as macrophytes provide important nesting and food resources for migrating waterfowl. Temporal changes in both vegetation and snail densities were compared between 2007 and 2015. Between these years, B . tentaculata densities have nearly quadrupled despite minor changes in vegetation abundance, distribution and composition. Understanding the spatial distribution of B . tentaculata in relation to other habitat features, including submersed vegetation, and quantifying any further changes in the abundance and distribution of B . tentaculata over time will be important for better identifying areas of risk for disease transmission to waterfowl.

River Research and Applications

Invasion of Asian tiger shrimp, Penaeus monodon Fabricius, 1798, in the western north Atlantic and Gulf of Mexico

After going unreported in the northwestern Atlantic Ocean for 18 years (1988 to 2006), the Asian tiger shrimp, Penaeus monodon , has recently reappeared in the South Atlantic Bight and, for the first time ever, in the Gulf of Mexico. Potential vectors and sources of this recent invader include: 1) discharged ballast water from its native range in Asia or other areas where it has become established; 2) transport of larvae from established non-native populations in the Caribbean or South America via ocean currents; or 3) escape and subsequent migration from active aquaculture facilities in the western Atlantic. This paper documents recent collections of P. monodon from the South Atlantic Bight and the Gulf of Mexico, reporting demographic and preliminary phylogenetic information for specimens collected between North Carolina and Texas from 2006 through 2012. The increased number of reports in 2011 and 2012, ranging from 102 mm to 298 mm total length, indicates that an adult population is present in densities sufficient for breeding, which is indicative of incipient establishment. Based on these reports of P. monodon , its successful invasion elsewhere, and its life history, we believe that this species will become common in the South Atlantic Bight and Gulf of Mexico in less than 10 years. Penaeus monodon is an aggressive predator in its native range and, if established, may prey on native shrimps, crabs, and bivalves. The impacts of an established P. monodon population are potentially widespread (e.g., alterations in local commercial fisheries, direct and indirect pressures on native shrimp, crab and bivalve populations, and subsequent impacts on the populations of other predators of those organisms) and should be considered by resource managers. The impacts of P. monodon on native fauna and the source(s) or vector(s) of the invasion, however, remain unknown at this time.

Atlantic Ocean;Gulf Of Mexico;South Atlantic Bight

Unusual population attributes of invasive red-eared slider turtles ( Trachemys scripta elegans ) in Japan: do they have a performance advantage?

The slider turtle ( Trachemys scripta Thunberg in Schoepff, 1792) is native to the USA and Mexico. Due to the popularity of their colorful hatchlings as pets, they have been exported worldwide and are now present on all continents, except Antarctica. Slider turtles are well-established in Japan and occupy aquatic habitats in urban and agricultural areas, to the detriment of native turtles with which they compete. We asked the overall question, do slider turtles in Japan have a performance advantage because they are liberated from the numerous competing turtle species in their native range and released from many of their natural predators? Traits compared included various measures of adult body size (mean, maximum), female size at maturity as measured by size of gravid females, clutch size, population density and biomass, sex ratio, and sexual size dimorphism, the latter two a partial reflection of growth and maturity differences between the sexes. We sampled slider turtle populations in three habitats in Japan and compared population attributes with published data for the species from throughout its native range in the USA. Mean male body sizes were at the lower end of values from the USA suggesting that males in Japan may mature at smaller body sizes. The smallest gravid females in Japan mature at smaller body sizes but have mean clutch sizes larger than some populations in the USA. Compared to most populations in the USA, slider turtles achieve higher densities and biomasses in Japanese wetlands, especially the lotic system we sampled. Sex ratios were female-biased, the opposite of what is reported for many populations in protected areas of the USA. Sexual size dimorphism was enhanced relative to native populations with females as the larger sex. The enhanced dimorphism is likely a result of earlier size of maturity in Japanese males and the large size of mature (gravid) Japanese females. Slider turtles appear to have a performance advantage over native turtles in Japan, possibly as a result of being released from competition with numerous sympatric turtle species in their native range, and the absence of many co-evolved predators and parasites in Japan. This slight competitive edge, coupled with the catholic diet and broad tolerance of varying aquatic habitats of slider turtles, is reflected in their dominance over native and naturalized Japanese turtles in altered aquatic habitats.

Aquatic Invasions

Population characteristics of silver carp from the source of their North American introduction in the Lower Mississippi River

Silver carp, Hypophthalmichthys molitrix , escaped into the Lower Mississippi River ( LMR ) over 50 years ago, established reproductive populations, and spread across much of the Mississippi River Basin. Demographic rates of silver carp are needed to inform decisions on control and management of this invasive species, but have not been published for the LMR . The purpose of this paper is to report silver carp age and growth estimates from fish collected in riverine (mainstem) and backwater (lake) habitats in the LMR during the period 2011–2019, to compare our results with populations from other geographic areas in the Upper Mississippi River drainage, and to evaluate latitudinal and habitat differences in demographic parameters. Silver carp gained weight with increasing length similarly throughout the lower and upper basin. However, annual growth rates were higher in the LMR compared to northern rivers including the Illinois, Wabash, Missouri, and Middle Mississippi rivers. In the LMR , regression analyses demonstrated that females were heavier in lakes than males or females in the mainstem and that females in lakes had the lowest instantaneous mortality (-0.186). Maximum age was 8 and 10 years for females and males, respectively. The largest male weighed 13.8 kg with a total length of 1022 mm, and was 7 years old. The largest female weighed 16.0 kg with a total length of 1034 mm TL, and was 7 years old. Rapid growth rates, larger sizes, and lower mortality in the LMR , in combination with limited commercial fishing, extensive river-floodplain connectivity, and vast amounts of spawning areas, ensure that LMR silver carp will continue to act as a source of fast-growing invasive individuals for other reaches and other rivers throughout the Mississippi River Basin.

Lower Mississippi River

Invasion frustration: Can biotic resistance explain the small geographic range of non-native croaking gourami Trichopsis vittata (Cuvier, 1831) in Florida, USA?

Croaking gourami Trichopsis vittata is a non-native fish species that has maintained a reproducing population in Florida, USA, since at least the 1970s. However, unlike most other non-native fishes in Florida, T. vittata has not spread beyond its very small (ca. 5 km²) range. We suspected the inability of T. vittata to colonize new habitats may be due to biotic resistance by the native eastern mosquitofish Gambusia holbrooki. In laboratory experiments, we show that G. holbrooki causes physical damage to T. vittata and that T. vittata’s growth is reduced in the presence of G. holbrooki. While the effects of G. holbrooki on T. vittata were sub-lethal, they were severe enough to hamper its growth and could affect recruitment in the wild. These results support the hypothesis that small non-native fishes may be excluded from establishment or may only establish small ranges due to pressure from G. holbrooki. Biotic resistance may reduce invasion success and thus consideration of species interactions is useful for natural resource managers trying to evaluate the potential risk of new invaders.

Florida

Occurrence of a reproducing wild population of Channa aurolineata (Pisces: Channidae) in the Manatee River drainage, Florida

We report on the discovery of a wild, reproducing population of Channa aurolineata (Pisces: Channidae) in west-central Florida (USA), and first documented occurrence of snakeheads in the Gulf Coast region. Channa aurolineata is a large, predatory fish of the bullseye snakehead “Marulius group” species complex from Asia. Adult and juvenile specimens were captured in June 2020 in a 1.8-hectare pond that connects during high water to a small stream within the Manatee River-Tampa Bay Basin. The pond site is 250-km from the only other wild C. aurolineata population in the USA (present in southeast Florida since ca. 2000) and is considered a separate introduction and not the result of natural dispersal. Morphological and molecular comparisons revealed high overlap between the two Florida populations, evidence humans may have transported fish between sites. To verify identification, we compared Florida samples to C. aurolineata from Thailand and found mtDNA-COI barcode sequences to be identical or to differ by only a single base pair. Life body coloration of Florida samples matched their Asian counterparts, but Florida specimens averaged fewer dorsal fin rays (53.6 vs. 56.0), anal fin rays (34.2 vs 36.1), lateral line scales (65.3 vs. 67.4), and vertebrae (62.1 vs. 64.3), differences implying possible founder effect or sampling bias. Existence of this invasive predator is a concern because of the risk of spread and negative ecological effects, including an observation of terrestrial hunting behavior. In 2020–2021, several hundred C. aurolineata were removed from the pond by nets and electrofishing, and surveys suggested the population had not spread to nearby waters. In May 2021 the pond was treated with rotenone and 48 more specimens were recovered. No additional snakeheads have been sighted since the piscicide operation, although verification of eradication will require monitoring of the watershed.

Florida

Assessment of suitable habitat for Phragmites australis (common reed) in the Great Lakes coastal zone

In the Laurentian Great Lakes, the invasive form of Phragmites australis (common reed) poses a threat to highly productive coastal wetlands and shorelines by forming impenetrable stands that outcompete native plants. Large, dominant stands can derail efforts to restore wetland ecosystems degraded by other stressors. To be proactive, landscape-level management of Phragmites requires information on the current spatial distribution of the species and a characterization of areas suitable for future colonization. Using a recent basin-scale map of this invasive plant’s distribution in the U.S. coastal zone of the Great Lakes, environmental data (e.g., soils, nutrients, disturbance, climate, topography), and climate predictions, we performed analyses of current and predicted suitable coastal habitat using boosted regression trees, a type of species distribution modeling. We also investigated differential influences of environmental variables in the upper lakes (Lakes Superior, Michigan, and Huron) and lower lakes (Lakes St. Clair, Erie, and Ontario). Basin-wide results showed that the coastal areas most vulnerable to Phragmites expansion were in close proximity to developed lands and had minimal topographic relief, poorly drained soils, and dense road networks. Elevated nutrients and proximity to agriculture also influenced the distribution of Phragmites . Climate predictions indicated an increase in suitable habitat in coastal Lakes Huron and Michigan in particular. The results of this study, combined with a publicly available online decision support tool, will enable resource managers and restoration practitioners to target and prioritize Phragmites control efforts in the Great Lakes coastal zone.

Great Lakes

Seasonal dynamics of zooplankton in Columbia–Snake River reservoirs,with special emphasis on the invasive copepod Pseudodiaptomus forbesi

The Asian copepod Pseudodiaptomus forbesi has recently become established in the Columbia River. However, little is known about its ecology and effects on invaded ecosystems. We undertook a 2-year (July 2009 to June 2011) field study of the mesozooplankton in four reservoirs in the Columbia and Snake Rivers, with emphasis on the relation of the seasonal variation in distribution and abundance of P. forbesi to environmental variables. Pseudodiaptomus forbesi was abundant in three reservoirs; the zooplankton community of the fourth reservoir contained no known non-indigenous taxa. The composition and seasonal succession of zooplankton were similar in the three invaded reservoirs: a bloom of rotifers occurred in spring, native cyclopoid and cladoceran species peaked in abundance in summer, and P. forbesi was most abundant in late summer and autumn. In the uninvaded reservoir, total zooplankton abundance was very low year-round. Multivariate ordination indicated that temperature and dissolved oxygen were strongly associated with zooplankton community structure, with P. forbesi appearing to exhibit a single generation per year . The broad distribution and high abundance of P. forbesi in the Columbia–Snake River System could result in ecosystem level effects in areas intensively managed to improve conditions for salmon and other commercially and culturally important fish species.

Columbia River, Snake River

Invasion of American bullfrogs along the Yellowstone River

The American bullfrog ( Lithobates catesbeianus ) is a globally distributed invasive species that was introduced to the Yellowstone River floodplain of Montana. Knowledge about floodplain habitat features that allow for bullfrog persistence and spread will help identify effective control strategies. We used field surveys in 2010, 2012 and 2013 to describe bullfrog spread in the Yellowstone River floodplain and the habitat features that are associated with bullfrog occupancy and colonization. Bullfrogs in our study area expanded from ~ 60 km in 2010 to 106 km in 2013, and are spreading to up- and downstream habitats. The number of breeding sites (i.e., presence of bullfrog eggs or larvae) increased from 12 sites in 2010 to 45 sites in 2013. We found that bullfrogs were associated with deeper waters, emergent vegetation and public-access sites, which are habitat features that characterize permanent waters and describe human-mediated introductions. Control strategies that reduce the hydroperiod of breeding sites may help to limit bullfrog persistence and spread, while an increase in public outreach and education may help prevent further bullfrog introductions at public-access sites.

Montana

The invasive Asian benthic foraminifera Trochammina hadai Uchio, 1962: Identification of a new local in Normandy (France) and a discussion on its putative introduction pathways

The invasive benthic foraminifera Trochammina hadai has been found for the first time in Europe along the coast of Normandy. Its native range of distribution is in Asia (Japan and Korea), and it has also been introduced along the coasts of western North America, Brazil and Australia. Morphological and molecular assessments confirm that specimens found in Le Havre and Caen-Ouistreham harbors belong to the Asiatic type. Like in Asia, T. hadai was found in transitional waters with muddy sediments. It exhibited high relative abundances (up to about 40%) confirming that T. hadai is a highly competitive species. In the present study, it was nearly absent from natural transitional waters and very abundant in heavily modified habitats like harbors, suggesting that ballast waters may likely be the vector of introduction. It was not recorded farther north along the coast of the Hauts-de-France. It is further hypothesized that the finding of a few specimens outside the harbor may facilitate the expansion of T. hadai in the English Channel by means of propagules dispersion.

Normandy

Survival and growth of invasive Indo-Pacific lionfish at low salinities

Invasive Indo-Pacific lionfish [ Pterois volitans (Linnaeus, 1758) and P. miles (Bennett, 1828)] are now established throughout the Western North Atlantic. Several studies have documented negative effects of lionfish on marine fauna including significant changes to reef fish community composition. Established populations of lionfish have been documented in several estuaries, and there is concern that the species may invade other low-salinity environments where they could potentially affect native fauna. To gain a better understanding of their low-salinity tolerance, we exposed lionfish to four salinities [5, 10, 20 and 34 (control)]. No lionfish mortality was observed at salinities of 34, 20 or 10, but all fish died at salinity = 5 within 12 days. Lionfish survived for at least a month at a salinity of 10 and an average of about a week at 5. Fish started the experiment at an average mass of 127.9 g, which increased at a rate of 0.55 g per day while they were alive, regardless of salinity treatment. Our research indicated lionfish can survive salinities down to 5 for short periods and thus may penetrate and persist in a variety of estuarine habitats. Further study is needed on effects of salinity levels on early life stages (eggs, larvae).

Aquatic Invasions

Discovery of the invasive Mayan Cichlid fish "Cichlasoma" urophthalmus (Günther 1862) in Thailand, with comments on other introductions and potential impacts

We report on the occurrence and possible establishment of a non-native cichlid fish in a brackish-water system in the lower Chao Phraya River delta region, Thailand. Although, the possibility of some degree of introgressive hybridization can not be ruled out, Thailand specimens agree best with Mayan Cichlid “Cichlasoma” urophthalmus (Günther 1862). Our collections represent the first records of this New World, highly-invasive, euryhaline fish from Thailand and coincides with recent collections from Singapore. Positive identification of specimens as “C.” urophthalmus requires caution due to the diversity of the Cichlidae (>1,300 species), widespread introduction of many family members, variation within species, extensive interspecific overlap in characters, and proliferation of artificial cichlid hybrids (e.g., Flowerhorns). We first became aware of the Thailand population in 2005 when “C.” urophthalmus began appearing in the catches of local fishermen. We visited the site in November 2006 and obtained and examined voucher specimens. The abundance and wide size range of juveniles and adults in local ponds and an adjacent canal is evidence of natural reproduction. Because water bodies throughout the Chao Phraya delta are interconnected and subject to flooding, it is likely that “C.” urophthalmus is already established and is dispersing, but surveys and monitoring are needed to determine their exact geographic range. The Thailand population is compared to “C.” urophthalmus introduced into Florida (USA). Based on what is known about Florida “C.” urophthalmus , it is predicted that this cichlid will further invade coastal and inland waters in Thailand and elsewhere in Southeast Asia. This cichlid has a long history in the aquarium trade in Europe. However, there are no records from the wild in European waters and, because of the colder climate, the possibility of establishment in that region is relatively low.

Chao Phraya River Delta

Spatio-temporal spawning and larval dynamics of a zebra mussel (Dreissena polymorpha) population in a North Texas Reservoir: implications for invasions in the southern United States

Zebra mussels were first observed in Texas in 2009 in a reservoir (Lake Texoma) on the Texas-Oklahoma border. In 2012, an established population was found in a near-by reservoir, Ray Roberts Lake, and in June 2013, settled mussels were detected in a third north Texas reservoir, Lake Lewisville. An established population was detected in Belton Lake in September 2013. With the exception of Louisiana, these occurrences in Texas mark the current southern extent of the range of this species in the United States. Previous studies indicate that zebra mussel populations could be affected by environmental conditions, especially increased temperatures and extreme droughts, which are characteristic of surface waters of the southern and southwestern United States. Data collected during the first three years (2010–12) of a long-term monitoring program were analyzed to determine if spatio-temporal zebra mussel spawning and larval dynamics were related to physicochemical water properties in Lake Texoma. Reproductive output of the local population was significantly related to water temperature and lake elevation. Estimated mean date of first spawn in Lake Texoma was approximately 1.5 months earlier and peak veliger densities were observed two months earlier than in Lake Erie. Annual maximum veliger density declined significantly during the study period (p < 0.0001). A population crash occurred as a result of thermal stress and variability of lake elevation. In summer 2011, water temperatures peaked at 34.3°C and lake elevation declined to the lowest level recorded during the previous 18 years, which resulted in desiccation of substantial numbers of settled mussels in littoral zones. Veliger spatial distributions were associated with physicochemical stratification characteristics. Veligers were observed in the deepest oxygenated water after lake stratification, which occurred in late spring. Results of this study indicate environmental conditions can influence variability of population sizes and spatial distributions of zebra mussels along the current southern frontier of their geographic range. Although the future population size trajectory and geographic range are uncertain, increased temperatures and intermittent, extreme droughts likely will affect spatio-temporal dynamics of established populations if zebra mussels spread farther into the southern and southwestern United States.

Oklahoma;Texas

Fort Collins Science Center-Fiscal year 2009 science accomplishments

Public land and natural resource managers in the United States are confronted with increasingly complex decisions that have important ramifications for both ecological and human systems. The scientists and technical professionals at the U.S. Geological Survey Fort Collins Science Center?many of whom are at the forefront of their fields?possess a unique blend of ecological, socioeconomic, and technological expertise. Because of this diverse talent, Fort Collins Science Center staff are able to apply a systems approach to investigating complicated ecological problems in a way that helps answer critical management questions. In addition, the Fort Collins Science Center has a long record of working closely with the academic community through cooperative agreements and other collaborations. The Fort Collins Science Center is deeply engaged with other U.S. Geological Survey science centers and partners throughout the Department of the Interior. As a regular practice, we incorporate the expertise of these partners in providing a full complement of ?the right people? to effectively tackle the multifaceted research problems of today's resource-management world. In Fiscal Year 2009, the Fort Collins Science Center's scientific and technical professionals continued research vital to Department of the Interior's science and management needs. Fort Collins Science Center work also supported the science needs of other Federal and State agencies as well as non-government organizations. Specifically, Fort Collins Science Center research and technical assistance focused on client and partner needs and goals in the areas of biological information management and delivery, enterprise information, fisheries and aquatic systems, invasive species, status and trends of biological resources (including human dimensions), terrestrial ecosystems, and wildlife resources. In the process, Fort Collins Science Center science addressed natural-science information needs identified in the U.S. Geological Survey Science Strategy (http://www.usgs.gov/science_strategy), including understanding and predicting change in ecosystems, climate variability and change, energy development and land management, the role of the environment and wildlife in human health, freshwater ecosystems, data integration, and evolving technologies. Several science projects were expanded in Fiscal Year 2009 to meet these evolving needs.

Open-File Report

Dynamics of Potamopyrgus antipodarum infestations and seasonal water temperatures in a heavily used recreational watershed in intermountain North America

Following the discovery of New Zealand mudsnails, Potamopyrgus antipodarum, in the Silver Creek watershed in Idaho, we investigated the distribution and dynamics of the snail populations over two years in field surveys. Despite extensive fishing and recreational activities in the watershed, the infestations appeared limited in extent. As with other published studies, densities of P. antipodarum were highest during summer months, but the distribution in Silver Creek was patchy. We found that near-to-below freezing winter water temperatures in localized reaches of the watershed were related to reduced populations or lack of detection. Distributions observed in winter were associated with regions of groundwater releases, or downstream of impoundments that dampened the temperature extremes observed in locations elsewhere in the watershed. We speculate that the population has remained restricted because thermal conditions are not conducive to year-round survival and growth. However, these relationships could be altered with watershed alterations or global climate change.

Idaho

Comparison of burbot populations across adjacent native and introduced ranges

Introduced species are a threat to biodiversity. Burbot, Lota lota, a fish native to the Wind River Drainage, Wyoming and a species of conservation concern, have been introduced into the nearby Green River Drainage, Wyoming, where they are having negative effects on native fish species. We compared these native and introduced burbot populations to evaluate potential mechanisms that could be leading to introduction success. We examined genetic ancestry, physical habitat characteristics, community composition, and burbot abundance, relative weight, and size structure between the native and introduced range to elucidate potential differences. The origin of introduced burbot in Flaming Gorge Reservoir is most likely Boysen Reservoir and several nearby river populations in the native Wind River Drainage. Burbot populations did not show consistent differences in abundance, size structure, and relative weight between drainages, though Fontenelle Reservoir, in the introduced drainage, had the largest burbot. There were also limited environmental and community composition differences, though reservoirs in the introduced drainage had lower species richness and a higher percentage of non-native fish species than the reservoir in the native drainage. Burbot introduction in the Green River Drainage is likely an example of reservoir construction creating habitat with suitable environmental conditions to allow a southwards range expansion of this cold-water species. An understanding of the factors driving introduction success can allow better management of species, both in their introduced and native range.

Wyoming