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Aboveground carbon stocks across a hydrological gradient: Ghost forests to non-tidal freshwater forested wetlands

Upper estuarine forested wetlands (UEFWs) play an important role in the sequestration of atmospheric carbon (C), which is facilitated by their position at the boundary of terrestrial and maritime environments but threatened by sea level rise. This study assessed the change in aboveground C stocks along the estuarine–riverine hydrogeomorphic gradient spanning salt-impacted freshwater tidal forested wetlands to freshwater forested wetlands in seasonally tidal and nontidal landscape positions. Standing stocks of C in forested wetlands were measured along two major coastal river systems, the Winyah Bay in South Carolina and the Savannah River in Georgia (USA), replicating and expanding a previous study to allow the assessment of change over time. Aboveground C stocks on these systems averaged 172.9 Mg C ha −1 , comparable to those found in UEFWs across the globe and distinct from the terrestrial forested ecosystems they are often considered to be a part of during large-scale C inventory efforts. Groundwater salinity conditions as low as 1.3 ppt were observed in conjunction with losses of aboveground C. When viewed in context alongside expected sea level rise and corresponding saltwater intrusion estimates, these data suggest a marked decrease in aboveground C stocks in forested wetlands situated in and around tidal estuaries.

Georgia, South Carolina↗

Using dissolved carbon dioxide to alter the behavior of invasive round goby

Fisheries managers need effective methods to limit the spread of invasive round goby Neogobius melanostomus in North America. Elevating carbon dioxide (CO 2 ) in water at pinch points of rivers (e.g., inside locks) is one approach showing potential to deter the passage of invasive fishes, such as bigheaded carps Hypophthalmichthys spp., but the effectiveness of this method to alter round goby behavior has not been determined. The goal for this study was to determine CO 2 concentrations that alter round goby behavior across a range of water temperatures. Free-swimming avoidance (voluntary response) and loss of equilibrium (involuntary response) were quantified by exposing round goby to increasing CO 2 concentrations at 5, 15, and 25 °C using a shuttle box choice arena and static tank. Water chemistry was measured concurrent with behavioral endpoints and showed that round goby avoided a threshold of 99–169 mg/L CO 2 (79,000–178,000 µatm) and lost equilibrium at 197–280 mg/L CO 2 (163,000–303,000 µatm). Approximately 50% lower CO 2 concentrations were found to modify behavior at 5 °C relative to 25 °C, suggesting greater effectiveness at lower water temperatures. We conclude that CO 2 modified round goby behavior and concentrations determined in this study are intended to guide field testing of CO 2 as an invasive fish deterrent.

Management of Biological Invasions↗

Field evaluation of carbon dioxide as a fish deterrent at a water management structure along the Illinois River

Construction of a water management structure (WMS) in the levee surrounding The Nature Conservancy’s Emiquon Preserve (Havana, Illinois, USA) created a new hydrological connection and potential aquatic invasive species pathway between the Illinois River and a large conservation wetland complex. Site managers need a control tool that deters the upstream passage of non-native fishes into the wetland lakes, but does not interfere with normal gate operation and water discharge. This short field study evaluated carbon dioxide (CO 2 ) injected into water as a non-obstructive method to reduce fish abundance near the WMS culverts. We quantified relative fish abundance using underwater sonar with and without injection of CO 2 into culverts during three discharge events: no flow (0 m 3 /s), restricted flow (0.9 m 3 /s), and unrestricted flow (3.2 m 3 /s). Overall, CO 2 reached or exceeded our target concentration of 100 mg/L during no flow and restricted flow, and fish abundance was 70–95% lower at culvert entrances relative to untreated control days. The target CO 2 level was not reached during unrestricted flow and fish abundance was not reduced during CO 2 injection. Atmospheric CO 2 concentrations were inconsequential and unaffected by CO 2 treatments throughout testing. Results from this initial field study provide several considerations for CO 2 as a fish deterrent in natural environments.

Illinois↗

Use of carbon dioxide in zebra mussel (Dreissena polymorpha) control and safety to a native freshwater mussel (Fatmucket, Lampsilis siliquoidea)

Control technology for dreissenid mussels ( Dreissena polymorpha and D. bugensis ) currently relies heavily on chemical molluscicides that can be both costly and ecologically harmful. There is a need for more environmentally neutral tools to manage dreissenid mussels, particularly in cooler water. Carbon dioxide (CO 2 ) has been shown to be lethal to several species of invasive bivalves, including zebra mussels and Asian clams ( Corbicula fluminea ). We evaluated the effectiveness of unpressurized infusion of CO 2 for 24 to 96 h (100 000–300 000 µatm PCO 2 ) at a water temperature of 12 °C on mortality, byssal thread formation, and attachment of zebra mussels. The safety of elevated CO 2 to a nontarget native freshwater mussel (Fatmucket, Lampsilis siliquoidea ) was also determined. Elevated PCO 2 exposure induced narcotization and reduced attachment of zebra mussels within 24 h. Mortality increased with exposure duration and PCO 2 . An estimated LT50 (lethal time to produce 50% mortality) for fixed PCO 2 ranged from 24 h at 275 000 µatm to ~ 96 h at 100 000 µatm. Exposure of zebra mussels to CO 2 for 96 h caused 80–100% mortality at all treatment levels. Fatmucket juveniles survived all PCO 2 treatments but burial and byssal thread production were adversely affected during exposure. Our results demonstrate that CO 2 is a viable option for management of zebra mussels in cool water and may have less adverse effect for native lampsiline mussels than current-use molluscicides.

Management of Biological Invasions↗

Laboratory trials to evaluate carbon dioxide as a potential behavioral control method for invasive red swamp (Procambarus clarkii) and rusty crayfish (Faxonius rusticus)

Few effective strategies are available to control invasive crayfishes. Carbon dioxide (CO 2 ) acts as a behavioral deterrent for invasive fishes and could be a useful crayfish control tool. The objective of this laboratory study was to quantify CO 2 concentrations that caused red swamp crayfish (RSC; Procambarus clarkii ) and rusty crayfish (RYC; Faxonius rusticus ) avoidance behavior, altered emergence behavior, and caused loss of equilibrium. Behavioral endpoints were quantified under light and dark conditions and at 10 and 24 °C. Avoidance responses from both species varied widely. Under light conditions, 35 mg/L CO 2 was needed to induce the first avoidance shuttle in both crayfish species at 10 °C. CO 2 concentrations of 42 mg/L for RYC and 46 mg/L for RSC were required for first shuttle at 24 °C. The first avoidance shuttle was induced at 37 mg/L CO 2 for RYC and 54 mg/L CO 2 for RSC at 10 °C in the dark. At 24 °C, 44 mg/L CO 2 was required for first shuttle for both species. Less CO 2 was needed to cause the last avoidance shuttle in RYC compared to RSC at both temperatures and under both lighting conditions. RSC emergence occurred at 418 ± 77 mg/L CO 2 , and loss of equilibrium occurred for both species at 1,231 ± 201 mg/L CO 2 . RYC appeared to be more sensitive than RSC to CO 2 , but behavior did not differ among light and water temperature treatments. These results demonstrate that CO 2 alters crayfish behavior. The CO 2 concentrations identified during this study may inform field testing to develop CO 2 as a potential control tool for invasive crayfishes.

Biological Invasions↗

Use of carbon dioxide to prevent zebra mussel (Dreissena polymorpha) settlement and effects on native mussels (Order Unionoida) and benthic communities

We determined the efficacy of carbon dioxide (CO2) for preventing larval (veliger) settlement of the invasive zebra mussel (Dreissena polymorpha) and compared the response of native juvenile mussels and macroinvertebrate and periphyton communities to hypercapnia. A microcosm system of control (ambient Camb) and CO2 treatments [low PCO2 (Clow)=35,094 µatm, and high PCO2 (Chigh)=66,685 µatm)] was supplied with river water for 11 weeks. We measured zebra mussel settlement, juvenile native mussel (Lampsilis cardium and L. siliquoidea) growth and condition, and macroinvertebrate and periphyton community composition. Zebra mussels settled in all Camb tanks (n=4) from early July through August compared to one mussel in a CO2 treatment tank over the same period. Native mussel growth, tissue condition, and shell condition were reduced in CO2, particularly in the highest treatment. Benthic biomass (excluding zebra mussels) was similar in Camb and Clow but was reduced in Chigh. Macroinvertebrate community composition differed among treatments due to greater abundance of Chironomidae in Clow and Oligochaeata in Chigh. Periphyton abundance and richness increased in both CO2 treatments and was driven by increases in Cyanobacteria, Bacillariophyta and Chlorophyta. Our results indicate that efficacious levels of CO2 (~35,000 µatm PCO2) for reducing biofouling by dreissenids are tolerable to most freshwater benthic taxa. Chronic elevated CO2 infusion (>35,000 µatm PCO2) may reduce native mussel growth and condition and alter benthic invertebrate and periphyton community composition. Further refinement of CO2 application strategies is needed to determine minimal effective dose and duration to prevent dreissenid settlement and minimize effects on nontarget organisms.

Management of Biological Invasions↗

Carbon dioxide (CO2) gas and eDNA monitoring as tools for eradicating invasive fish from anchialine pools in Hawai‘i

Invasive fish can profoundly affect communities they invade. In Hawai‘i, invasive fishes have become established in many anchialine pools, threatening the persistence of resident invertebrates, including several endangered species. Tools to eradicate invasive fishes from these pools are lacking. This study tested the efficacy of carbon dioxide (CO2) gas diffused into anchialine pool water as a method to eradicate invasive Mozambique tilapia (Oreochromis mossambicus), guppies (Poecilia reticulata), and western mosquitofish (Gambusia affinis). We first conducted aquarium trials to identify how these fishes were affected by elevated CO2 and the concomitant reduction in pH. We then carried out field trials in pools containing these fish in one pool each at two national historical parks on the Island of Hawai‘i during July 2021–January 2022. We also developed environmental DNA (eDNA) protocols to detect fish that may have survived CO2 treatments. The effect of CO2 on fish behavior varied among species; at pH 5.3 (CO2 = 255 mg/L) for tilapia and 5.0 (CO2 = 488 mg/L) for tilapia, guppies, and mosquitofish, all generally lost their ability to swim, showed slow or no gill movement, and altered their position in the water column. No tilapia survived the trials (n = 4 and 6 individuals at pH 5.3 and 5.0, respectively). In contrast, 41.7% (n = 12) of adult guppies and 66.7% (n = 12) of adult mosquitofish survived treatment at pH 5.0. In the field we were unable to reduce anchialine pool water pH below 5.7. Regardless, we were able to eradicate tilapia from one pool over four sequential treatments. Post-treatment eDNA assessments supported visual surveys, confirming our results. We were not able to eradicate guppies and mosquitofish. Results from this study show that CO2 can be an effective tool for eradicating invasive tilapia from anchialine pools, and post-treatment eDNA assessments can provide managers with a method for evaluating the success of eradication efforts.

Hawaii↗

Carbon dioxide-induced mortality of four species of North American fishes

Fisheries managers have a growing interest in the use of carbon dioxide (CO 2 ) as a tool for controlling invasive fishes. However, limited published data exist on susceptibility of many commonly encountered species to elevated CO 2 concentrations. Our objective was to estimate the 24-h 50% lethal concentration (LC 50 ) and 95% lethal concentration (LC 95 ) of CO 2 for four fishes (Rainbow Trout Oncorhynchus mykiss , Common Carp Cyprinus carpio , Channel Catfish Ictalurus punctatus , and Westslope Cutthroat Trout Oncorhynchus clarkii lewisi ). In the laboratory, we exposed juvenile fish to a range of CO 2 concentrations for 24 h in unpressurized, flow-through tanks. We developed a Bayesian hierarchical model to estimate the dose-response relationship for each fish species with associated uncertainty, and estimated 24-h LC 50 and LC 95 values based on laboratory trials for each species. The minimum concentration inducing mortality differed among cold water–adapted species and warm water–adapted species groups: 150 mg CO 2 /L for Westslope Cutthroat Trout and Rainbow Trout and 225 mg CO 2 /L for Common Carp and Channel Catfish. We observed complete mortality at 275 mg CO 2 /L (38,672 microatmospheres [μatm]), 225 mg CO 2 /L (30,711 μatm), and 495 mg CO 2 /L (65,708 μatm [Common Carp]; 77,213 μatm [Channel Catfish]) for Westslope Cutthroat Trout, Rainbow Trout, and both Common Carp and Channel Catfish, respectively. There was evidence of a statistical difference between the 24-h LC 95 values of Westslope Cutthroat Trout and Rainbow Trout (245.0 [222.2–272.2] and 190.6 [177.2–207.8] mg CO 2 /L, respectively). Additionally, these values were almost half the estimated 24-h LC 95 values for Common Carp and Channel Catfish (422.5 [374.7–474.5] and 434.2 [377.2–492.2] mg CO 2 /L, respectively). Although the experimental findings show strong relationships between increased CO 2 concentration and higher mortality, additional work is required to assess the efficacy and feasibility of a CO 2 application in a field setting.

Journal of Fish and Wildlife Management↗

An empirical model of the phytoplankton chlorophyll : carbon ratio-the conversion factor between productivity and growth rate

We present an empirical model that describes the ratio of phytoplankton chlorophyll a to carbon, Chl: C, as a function of temperature, daily irradiance, and nutrient-limited growth rate. Our model is based on 219 published measurements of algal cultures exposed to light-limited or nutrient-limited growth conditions. We illustrate an approach for using this estimator of Chl: C to calculate phytoplankton population growth rate from measured primary productivity. This adaptive Chl: C model gives rise to interactive light-nutrient effects in which growth efficiency increases with nutrient availability under low-light conditions. One implication of this interaction is the enhancement of phytoplankton growth efficiency, in addition to enhancement of biomass yield, as a response to eutrophication.

Limnology and Oceanography↗

The role of effective discharge in the ocean delivery of particulate organic carbon by small, mountainous river systems

Recent research has shown that small, mountainous river systems (SMRS) account for a significant fraction of the global flux of sediment and particulate organic carbon (POC) to the ocean. The enormous number of SMRS precludes intensive studies of the sort conducted on large systems, necessitating development of a conceptual framework that permits cross‐system comparison and scaling up. Herein, we introduce the geomorphic concept of effective discharge to the problem of source‐to‐sink POC transport. This idea recognizes that transport effectiveness is the product of discharge frequency and magnitude, wherein the latter is quantified as a power‐law relationship between discharge and load (the ‚rating curve’). An analytical solution for effective discharge ( Q e ) identifies two key variables: the standard deviation of the natural logarithm of discharge (Σ q ), and the rating exponent of constituent i ( b i ). Data from selected SMRS are used to show that for a given river Q e ‐POC , Q e ‐sediment, Q e for different POC constituents (e.g., POC fossil vs. POC modern ) differs in predictable ways, and Q e for a particular constituent can vary seasonally. When coupled with the idea that discharge peaks of small rivers may be coincident with specific oceanic conditions (e.g., large waves, wind from a certain direction) that determine dispersal and burial, these findings have potentially important implications for POC fate on continental margins. Future studies of POC transport in SMRS should exploit the conceptual framework provided herein and seek to identify how constituent‐specific effective discharges vary between rivers and respond to perturbations.

Limnology and Oceanography↗

Dissolved organic carbon concentration controls benthic primary production: results from in situ chambers in north-temperate lakes

We evaluated several potential drivers of primary production by benthic algae (periphyton) in north-temperate lakes. We used continuous dissolved oxygen measurements from in situ benthic chambers to quantify primary production by periphyton at multiple depths across 11 lakes encompassing a broad range of dissolved organic carbon (DOC) and total phosphorous (TP) concentrations. Light-use efficiency (primary production per unit incident light) was inversely related to average light availability (% of surface light) in 7 of the 11 study lakes, indicating that benthic algal assemblages exhibit photoadaptation, likely through physiological or compositional changes. DOC alone explained 86% of the variability in log-transformed whole-lake benthic production rates. TP was not an important driver of benthic production via its effects on nutrient and light availability. This result is contrary to studies in other systems, but may be common in relatively pristine north-temperate lakes. Our simple empirical model may allow for the prediction of whole-lake benthic primary production from easily obtained measurements of DOC concentration.

Limnology and Oceanography↗

Decadal and long-term boreal soil carbon and nitrogen sequestration rates across a variety of ecosystems

Boreal soils play a critical role in the global carbon (C) cycle; therefore, it is important to understand the mechanisms that control soil C accumulation and loss for this region. Examining C & nitrogen (N) accumulation rates over decades to centuries may provide additional understanding of the dominant mechanisms for their storage, which can be masked by seasonal and interannual variability when investigated over the short term. We examined longer-term accumulation rates, using 210 Pb and 14 C to date soil layers, for a wide variety of boreal ecosystems: a black spruce forest, a shrub ecosystem, a tussock grass ecosystem, a sedge-dominated ecosystem, and a rich fen. All ecosystems had similar decadal C accumulation rates, averaging 84 ± 42 gC m −2 yr −1 . Long-term (century) C accumulation rates were slower than decadal rates, averaging 14 ± 5 gC m −2 yr −1 for all ecosystems except the rich fen, for which the long-term C accumulation rates was more similar to decadal rates (44 ± 5 and 76 ± 9 gC m −2 yr −1 , respectively). The rich fen also had the highest long-term N accumulation rates (2.7 gN m −2 yr −1 ). The lowest N accumulation rate, on both a decadal and long-term basis, was found in the black spruce forest (0.2 and 1.4 gN m −2 yr −1 , respectively). Our results suggest that the controls on long-term C and N cycling at the rich fen is fundamentally different from the other ecosystems, likely due to differences in the predominant drivers of nutrient cycling (oxygen availability, for C) and reduced amounts of disturbance by fire (for C and N). This result implies that most shifts in ecosystem vegetation across the boreal region, driven by either climate or succession, will not significantly impact regional C or N dynamics over years to decades. However, ecosystem transitions to or from a rich fen will promote significant shifts in soil C and N storage.

Biogeosciences↗

Reviews and syntheses: Field data to benchmark the carbon cycle models for tropical forests

For more accurate projections of both the global carbon (C) cycle and the changing climate, a critical current need is to improve the representation of tropical forests in Earth system models. Tropical forests exchange more C, energy, and water with the atmosphere than any other class of land ecosystems. Further, tropical-forest C cycling is likely responding to the rapid global warming, intensifying water stress, and increasing atmospheric CO 2 levels. Projections of the future C balance of the tropics vary widely among global models. A current effort of the modeling community, the ILAMB (International Land Model Benchmarking) project, is to compile robust observations that can be used to improve the accuracy and realism of the land models for all major biomes. Our goal with this paper is to identify field observations of tropical-forest ecosystem C stocks and fluxes, and of their long-term trends and climatic and CO 2 sensitivities, that can serve this effort. We propose criteria for reference-level field data from this biome and present a set of documented examples from old-growth lowland tropical forests. We offer these as a starting point towards the goal of a regularly updated consensus set of benchmark field observations of C cycling in tropical forests.

Biogeosciences↗

Impacts of exotic mangrove forests and mangrove deforestation on carbon remineralization and ecosystem functioning in marine sediments

To evaluate how mangrove invasion and removal can modify short-term benthic carbon cycling and ecosystem functioning, we used stable-isotopically labeled algae as a deliberate tracer to quantify benthic respiration and C-flow over 48 h through macrofauna and bacteria in sediments collected from (1) an invasive mangrove forest, (2) deforested mangrove sites 2 and 6 years after removal of above-sediment mangrove biomass, and (3) two mangrove-free control sites in the Hawaiian coastal zone. Sediment oxygen consumption (SOC) rates averaged over each 48 h investigation were significantly greater in the mangrove and mangrove removal site experiments than in controls and were significantly correlated with total benthic (macrofauna and bacteria) biomass and sedimentary mangrove biomass (SMB). Bacteria dominated short-term C-processing of added microalgal-C and benthic biomass in sediments from the invasive mangrove forest habitat and in the 6-yr removal site. In contrast, macrofauna were the most important agents in the short-term processing of microalgal-C in sediments from the 2-yr mangrove removal site and control sites. However, mean faunal abundance and C-uptake rates in sediments from both removal sites were significantly higher than in control cores, which collectively suggest that community structure and short-term C-cycling dynamics of sediments in habitats where mangroves have been cleared can remain fundamentally different from un-invaded mudflat sediments for at least 6-yrs following above-sediment mangrove removal. In summary, invasion by mangroves can lead to dramatic shifts in benthic ecosystem function, with sediment metabolism, benthic community structure and short-term C-remineralization dynamics being affected for years following invader removal.

Biogeosciences↗

Aeolian nutrient fluxes following wildfire in sagebrush steppe: Implications for soil carbon storage

Pulses of aeolian transport following fire can profoundly affect the biogeochemical cycling of nutrients in semi-arid and arid ecosystems. Our objective was to determine horizontal nutrient fluxes occurring in the saltation zone during an episodic pulse of aeolian transport that occurred following a wildfire in a semi-arid sagebrush steppe ecosystem in southern Idaho, USA. We also examined how temporal trends in nutrient fluxes were affected by changes in particle sizes of eroded mass as well as nutrient concentrations associated with different particle size classes. In the burned area, total carbon (C) and nitrogen (N) fluxes were as high as 235 g C m????'1 d????'1 and 19 g N m????'1 d????'1 during the first few months following fire, whereas C and N fluxes were negligible in an adjacent unburned area throughout the study. Temporal variation in C and N fluxes following fire was largely attributable to the redistribution of saltation-sized particles. Total N and organic C concentrations in the soil surface were significantly lower in the burned relative to the unburned area one year after fire. Our results show how an episodic pulse of aeolian transport following fire can affect the spatial distribution of soil C and N, which, in turn, can have important implications for soil C storage. These findings demonstrate how an ecological disturbance can exacerbate a geomorphic process and highlight the need for further research to better understand the role aeolian transport plays in the biogeochemical cycling of C and N in recently burned landscapes. ?? Author(s) 2011. CC Attribution 3.0 License.

Biogeosciences↗

Sensitivity analysis of the GEMS soil organic carbon model to land cover land use classification uncertainties under different climate scenarios in Senegal

Spatially explicit land cover land use (LCLU) change information is needed to drive biogeochemical models that simulate soil organic carbon (SOC) dynamics. Such information is increasingly being mapped using remotely sensed satellite data with classification schemes and uncertainties constrained by the sensing system, classification algorithms and land cover schemes. In this study, automated LCLU classification of multi-temporal Landsat satellite data were used to assess the sensitivity of SOC modeled by the Global Ensemble Biogeochemical Modeling System (GEMS). The GEMS was run for an area of 1560 km 2 in Senegal under three climate change scenarios with LCLU maps generated using different Landsat classification approaches. This research provides a method to estimate the variability of SOC, specifically the SOC uncertainty due to satellite classification errors, which we show is dependent not only on the LCLU classification errors but also on where the LCLU classes occur relative to the other GEMS model inputs.

Biogeosciences↗

Aeolian nutrient fluxes following wildfire in sagebrush steppe: Implications for soil carbon storage

. Pulses of aeolian transport following fire can profoundly affect the biogeochemical cycling of nutrients in semi-arid and arid ecosystems. Our objective was to determine horizontal nutrient fluxes occurring in the saltation zone during an episodic pulse of aeolian transport that occurred following a wildfire in a semi-arid sagebrush steppe ecosystem in southern Idaho, USA. We also examined how temporal trends in nutrient fluxes were affected by changes in particle sizes of eroded mass as well as nutrient concentrations associated with different particle size classes. In the burned area, total carbon (C) and nitrogen (N) fluxes were as high as 235 g Cm -1 d -1 and 19 g N m -1 d -1 during the first few months following fire, whereas C and N fluxes were negligible in an adjacent unburned area throughout the study. Temporal variation in C and N fluxes following fire was largely attributable to the redistribution of saltation-sized particles. Total N and organic C concentrations in the soil surface were significantly lower in the burned relative to the unburned area one year after fire. Our results show how an episodic pulse of aeolian transport following fire can affect the spatial distribution of soil C and N, which, in turn, can have important implications for soil C storage. These findings demonstrate how an ecological disturbance can exacerbate a geomorphic process and highlight the need for further research to better understand the role aeolian transport plays in the biogeochemical cycling of C and N in recently burned landscapes.

Idaho↗

Generalized models to estimate carbon and nitrogen stocks of organic soil horizons in Interior Alaska

Boreal ecosystems comprise one tenth of the world’s land surface and contain over 20 % of the global soil carbon (C) stocks. Boreal soils are unique in that its mineral soil is covered by what can be quite thick layers of organic soil. These organic soil layers, or horizons, can differ in their state of decomposition, source vegetation, and disturbance history. These differences result in varying soil properties (bulk density, C concentration, and nitrogen (N) concentration) among soil horizons. Here we summarize these soil properties, as represented by over 3000 samples from Interior Alaska, and examine how soil drainage and stand age affect these attributes. The summary values presented here can be used to gap-fill large datasets when important soil properties were not measured, provide data to initialize process-based models, and validate model results. These data are available at https://doi.org/10.5066/P960N1F9 (Manies, 2019).

Alaska↗