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Elk monitoring in Lewis and Clark National Historical Park: 2008-2012 synthesis report

Maintaining elk (Cervus elaphus roosevelti) herds that frequent Lewis and Clark National Historical Park (NHP) is central to the park’s purpose of preserving the historic, cultural, scenic, and natural resources associated with the winter encampment of the Lewis and Clark expedition. Elk were critically important to the Lewis and Clark expedition in providing food and hides that sustained the expedition during the winter of 1805-06 and supplied them for their return east during 1806. Today, elk remain a key component of interpreting the Lewis and Clark story to over 200,000 park visitors each year at the Fort Clatsop visitor center. In 2008, the US Geological Survey (USGS) began collaborating with Lewis and Clark NHP and the NPS North Coast and Cascades Network to develop a protocol for monitoring long-term changes in the magnitude and spatial patterns of elk use within and adjacent to Lewis and Clark NHP (Griffin et al. 2011). Specific objectives of the monitoring program were to measure trends in (1) relative use of the Fort Clatsop unit by elk during winter; (2) the proportion of areas where elk sign is present in the Fort Clatsop unit in winter; and (3) the frequency of elk sightings from roads in and around the Fort Clatsop unit. This report synthesizes the results of the first four years of monitoring elk distribution and use in Lewis and Clark NHP from 2008-2012. We also present data from FY2012 (Appendix 1), in lieu of an annual report for that year. We used fecal pellet group surveys as the cornerstone for monitoring trends in both relative use of the Fort Clatsop Unit by elk and the proportion of areas where elk sign was present at the end of winter. We estimated pellet group density based on data collected from a network of fecal pellet plots distributed systematically throughout the unit. We developed a double observer sampling scheme that enabled us to estimate detection biases and improve the accuracy of pellet group density estimates. We computed the estimated detection probability for any pellet group observed; this probability was a function of the pellet group size and stage of decay, as well as lighting and vegetation conditions, and the number of observers (one or two) searching for pellets in that subplot. We then used these estimated detection probabilities to adjust the raw counts of the detected pellet groups to account for groups that likely went undetected under similar pellet and environmental conditions (each observed pellet group was weighted by the inverse of its estimated detection probability). We also used results from the late winter fecal pellet surveys to quantify the proportion of areas where elk pellets occurred (PAO), which was based on the presence of fecal pellet groups and estimation of detection biases (i.e., accounting for pellet groups that likely went undetected by both observers). In this synthesis, we report temporal trends in both pellet group density and PAO from 2008-2012, based on weighted linear regression analyses as well as spatial variation of pellet group densities over time. We completed late winter fecal pellet surveys at 61-66 plots annually, depending on yearly variation in access. We cleared fecal pellets at survey points in late October / early November each year and returned in late February / early March to count pellet groups left by elk over the winter. The estimated probability that a pellet group was detected by any one observer during late winter was affected most by the pellet group size and was less affected by decay class and lighting conditions. Per-observer detection probabilities ranged from as low as ~10-15% for single pellets to ~85-90% for pellet groups with 50 pellets. Average pellet group density in the Fort Clatsop unit ranged annually from 0.58 (+/- 1.43 standard error [SE]) to 0.93 (+/- 2.25 SE) pellet groups per 3-m radius subplot. Pellet group density declined over time, at approximately 8.8% per year (+/- 2.5% SE), but that slope was not statistically distinguishable from zero (2- tailed P=0.16). Following correction for detection biases, the proportion of surveyed points used by elk (i.e., PAO) ranged from 0.44 (+/- 0.07 SE) to 0.53 (+/- 0.07 SE) during the 4 winters. The estimated proportion of areas where elk pellets occurred (PAO) declined at a rate of 2.6% per year (+/- 1.2% per year SE), but that trend also was not statistically distinguishable from zero (2- tailed P=0.17). Statistical significance of a measure’s trend depends on both the magnitude (i.e., slope) of the observed trend and the number of years the trend continues in the same increasing or decreasing direction. Through simulation modeling we determined how many additional years of surveys would be required to reveal a statistically significant trend, based on the same trends in pellet group density and PAO, and associated variation, observed from 2009-2012. Assuming the same trends persist in the future, simulations indicated that there is a 70% probability that a statistically significant trend would be detected after two more years of conducting pellet group surveys. Relative use by elk during winter, as indexed by elk pellet group density, was generally greatest in the southeast region of the Fort Clatsop unit in or near the large freshwater marsh at the mouth of Colewort Creek and adjacent upland areas. Pellet group density was also higher than average in the north-central forested area, not far from a privately-owned pasture north of the park boundary. This spatial pattern in pellet group densities across the Fort Clatsop unit was consistent across all four years, although specific pellet group densities varied from year to year. Pellet group density declined significantly over time at two points in the southeast of the Fort Clatsop unit, even though pellet group density at those points remained higher than the unit average. Pellet group density increased significantly over time at one point in the north-central region, and at one point in the south-central region of the unit, indicating a slight shift in the distribution of elk use within the Fort Clatsop Unit over the four years. As an index of visitors’ opportunities to see elk in and around the Fort Clatsop Unit, we conducted replicated roadside elk surveys 3-5 times monthly during February, April, June, August, October and December 2008-2012. During each morning of survey, we searched for elk along four routes that totaled 32 km. We examined bimonthly trends in the numbers of elk groups seen, the total number of elk seen, and the observed composition ratios for those six months of the year. The average number of elk groups seen per survey ranged from 0.75 (+/- 0.32 SE) during February to a peak of 1.95 (+/- 0.36 SE) during June. Despite this seasonal variation in numbers of elk groups seen, the average total number of elk seen per morning was less variable. The average ratios of antlered elk to antlerless adult elk (i.e., bulls:cows) and calves to antlerless adult elk (i.e. calves:cows) varied seasonally, with the highest of both average ratios observed in August. We detected no significant trends in the average number of elk groups and total numbers of elk seen per survey from 2008-2012. Similarly, ratios of calves and antlered elk per antlerless elk did not differ over time. Elk groups were frequently seen from January to August in the southeast region of the Fort Clatsop unit, in the vicinity of Colewort Creek. Outside of NPS lands, we observed elk most frequently in open areas near the Astoria regional airport, in the pastures and forests immediately north of the Fort Clatsop unit and, prior to the construction of a residential development, in a pasture northwest of the Fort Clatsop unit. Elk monitoring at Lewis and Clark NHP is still in its initial years and additional monitoring will be required to verify trends that appear to be emerging. For example, the initial monitoring suggested incipient declining trends in both pellet group density and proportion of plots with pellets present, as well as, potentially, a small shift in elk distribution away from a new trail that was recently constructed in the southeast portion of the Fort Clatsop unit. Continued monitoring will aid in determining whether this local change in distribution persists (or, alternatively, resulted from short-term random variation), and whether there will be any positive or negative effect in the northern portion of the unit where a new trail has been constructed. High variability in road counts prevented our ability to find any clear trend in numbers or composition of elk observed in and near Fort Clatsop, but changes in the patterns of observations of elk from roadways suggest that residential development outside the park has reduced the available habitat for elk in some of the areas surveyed, and may have affected spatial use patterns of elk adjacent to some areas of the park. In addition to monitoring future effects of land use changes outside the park, continued monitoring may also prove useful for assessing elk responses to natural succession in forests disturbed by windthrow in December 2007 and to NPS vegetation management activities such as variable density thinning in the forest, trail development, and restoration at Otter Point tidal area and Colewort Creek Slough.

Oregon;Washington↗

Movement and habitat use by smallmouth bass Micropterus dolomieu velox in a dynamic Ozark Highlands riverscape

Stream fish movement in response to changing resource availability and habitat needs is important for fish growth, survival and reproduction. The authors used radio telemetry to evaluate individual movements, daily movement rates, home ranges and habitat-use characteristics of adult (278–464 mm L T ) Neosho smallmouth bass Micropterus dolomieu velox in three Ozark Highlands streams from June 2016 to February 2018. The authors quantified variation in movement and habitat use among seasons and streams and examined relations with select environmental cues ( i.e. , temperature and discharge), fish size and sex. Maximum movement distances were an order of magnitude greater in the larger Elk River (17.0 km) and Buffalo Creek (12.9 km) than in the smaller Sycamore Creek (1.71 km), were similar in both upstream and downstream directions and typically occurred during the spring. Most movement rates were ≤10 m day −1 in all streams and seasons, except for Elk River during spring. Ranking of linear mixed-effects models using AIC c supported that movement rates were much greater in spring and increased with stream size. Spring movement rate increased with discharge and water temperature; only weak relationships were apparent during other seasons. Increased variation in water temperature had a small negative effect on movement rate. Home range size was highly variable among individuals, ranging 45–15,061 m (median: 773 m), and was not related to fish size, sex, season or stream. Although some fish moved between rivers, this study's tagged fish did not use reservoir or associated interface habitat. Water temperatures used by this study's tagged fish followed seasonal patterns but indicated the use of thermal refugia during summer and winter. Deeper-water habitats were used in Buffalo Creek and in winter across all study streams, whereas greater velocities used in the Elk River likely reflect the increased use of run habitats. Use of pool habitats predominated among tagged fish, particularly in smaller streams. The results of this study indicate considerable heterogeneity in movement and habitat use within and among lotic populations of Neosho smallmouth bass. These findings suggest that population-specific management may be appropriate and highlight the importance of natural flow conditions ( i.e. , spring high flows) and connected habitats for this endemic sport fish, particularly in smaller streams.

Missouri, Oklahoma↗

Sediment classification and the characterization, identification, and mapping of geologic substrates for the glaciated Gulf of Maine seabed and other terrains, providing a physical framework for ecological research and seabed management

A geologic substrate is a surface (or volume) of sediment or rock where physical, chemical, and biological processes occur, such as the movement and deposition of sediment, the formation of bedforms, and the attachment, burrowing, feeding, reproduction, and sheltering of organisms. Seabed mapping surveys in the Stellwagen Bank region off Boston, Massachusetts, from 1993 to 2004 have led to the development of a methodology for characterizing, identifying, and mapping geologic substrates. The resulting high-resolution interpretive maps (1:25,000) show the distribution of substrates in a glaciated terrain of banks and basins in water depths of 30 to 185 meters. Data sources used to characterize substrates are multibeam sonar bathymetric and backscatter imagery to document seabed topography and patterns of sediment and rock distribution, grain-size analyses of sediment samples to determine substrate composition, and video and photographic imagery of the seabed to aid in the interpretation of multibeam sonar imagery and to provide information on substrate layering and mobility, seabed structures, and sediments and nonsediment materials that cannot be physically sampled. Sediment composition is a major property of many seabed substrates. Sediment grains belong to a continuum of grain-diameter sizes previously classified into grades (for example, fine sand, medium sand) and into aggregates (mud, sand, gravel). The definition of grade and aggregate boundaries in a classification is arbitrary, and a useful classification is limited to as few classes as are needed to effectively organize and apply information. For the purpose of mapping substrates, sediment grades and aggregates were simplified and re-classified into eight composite grades based on grain-size content, mode of transport, and ecological role. Five composite grades are identified using grain-size analysis and three are identified using video and photographic imagery of the seabed. Naturally occurring sediments contain various amounts of the aggregates mud, sand, and gravel. The separation of naturally occurring sediments into sediment classes, based on grain-size analysis, requires that limits be set on the amount of mud, sand, and gravel each class contains. Fifteen previously identified basic sediment classes provided interpretive information on sediment transport by emphasizing gravel content (a low 0.01-weight-percent threshold) and on winnowing processes based on the sand-to-mud ratio. The present study recognizes 20 basic sediment classes that are combinations of aggregates in which the lower limits for recognition of mud and sand are 10 weight percent and of gravel, 25 weight percent. These sediment classes can be made more specific by listing their content of the composite grades fine-grained sand (3 and 4 phi), which is transported in suspension, and coarse-grained sand (0, 1, and 2 phi), which is transported as bedload. Additional sediment classes and nonsediment classes that cannot be sampled are recognized on the basis of visual analysis of seabed video and photographic imagery and include pebble, cobble, and boulder gravel, rock outcrops, and shell beds, among others. Substrates are not classified because their properties are too varied for a classification to be concise and useful. Rather, substrates are characterized and identified by sediment grain-size composition (the sediment class); the distribution, in millimeters, of grain diameters in the sediment; the presence of nonsediments (for example, rock outcrops); substrate mobility based on the presence of sediment ripples; substrate layering (for example, a partial veneer of sand on gravel); and seabed structures. These properties have interpretive value by providing information about sedimentary processes acting on a substrate and about its ecological function. A geologic substrate, when it is associated with one or more species, is an important element of a habitat. This methodology was developed to map a glaciated terrain characterized by geologic substrates that typify a wide range of erosional and depositional sedimentary environments, and it likely will be useful for mapping substrates in other terrains. Substrate maps provide the physical framework required for identifying sediment transport processes, validating sediment transport models, studying the ecology of species and communities, and managing marine resources and seabed usage.

Massachusetts↗

Seasonal and species-level water-use strategies and groundwater dependence in dryland riparian woodlands during extreme drought

Drought-induced groundwater decline and warming associated with climate change are primary threats to dryland riparian woodlands. We used the extreme 2012–2019 drought in southern California as a natural experiment to assess how differences in water-use strategies and groundwater dependence may influence the drought susceptibility of dryland riparian tree species with overlapping distributions. We analyzed tree-ring stable carbon and oxygen isotopes collected from two cottonwood species ( Populus trichocarpa and P . fremontii ) along the semi-arid Santa Clara River. We also modeled tree source water δ 18 O composition to compare with observed source water δ 18 O within the floodplain to infer patterns of groundwater reliance. Our results suggest that both species functioned as facultative phreatophytes that used shallow soil moisture when available but ultimately relied on groundwater to maintain physiological function during drought. We also observed apparent species differences in water-use strategies and groundwater dependence related to their regional distributions. P . fremontii was constrained to more arid river segments and ostensibly used a greater proportion of groundwater to satisfy higher evaporative demand. P . fremontii maintained ∆ 13 C at pre-drought levels up until the peak of the drought, when trees experienced a precipitous decline in ∆ 13 C. This response pattern suggests that trees prioritized maintaining photosynthetic processes over hydraulic safety, until a critical point. In contrast, P . trichocarpa showed a more gradual and sustained reduction in ∆ 13 C, indicating that drought conditions induced stomatal closure and higher water use efficiency. This strategy may confer drought avoidance for P . trichocarpa while increasing its susceptibility to anticipated climate warming.

California↗

Promoting synergy in the innovative use of environmental data—Workshop summary

From December 2 to 4, 2015, NatureServe and the U.S. Geological Survey organized and hosted a biodiversity and ecological informatics workshop at the U.S. Department of the Interior in Washington, D.C. The workshop objective was to identify user-driven future directions and areas of collaboration in advanced applications of environmental data applied to forecasting and decision making for the sustainability of biodiversity and ecosystem services. Substantial effort to recruit attendees from diverse Federal, State, and private sector organizations successfully attracted participants from 20 Federal agencies and 48 different institutions in the academic, nonprofit, State government, and commercial sectors; the total number of attendees ranged from 100 to 144 during the 3-day workshop. The first one-half of the workshop was divided into 7 plenary sessions and 3 sets of lightning talk sessions organized by sector, providing 48 oral and visual plenary presentations that shared diverse perspectives on biodiversity and ecological informatics, including original biospatial analyses from 6 graduate student map contest winners. The second one-half of the workshop focused on 10 breakout sessions with participant-driven themes from the environmental data sphere and concluded with an address by the Director of the U.S. Fish and Wildlife Service. The workshop was structured to encourage interactivity. About 80–90 percent of attendees provided direct feedback using clicker devices for specific questions related to biodiversity and ecological data uses and needs, and 10 breakout session leaders shared the highlights of their group discussions during the final workshop plenary sessions. Participants were encouraged to use the Twitter hashtag #ShareUrData. Over lunch on day 2 there were 20 simultaneous presentations of tools and apps during a special “Tools Café” session. The 10 participant-defined breakout session topics are listed below: Ecosystem services and ecological indicators Inventory and monitoring Biogeographic map of the Nation Pollinators Invasive species Remote sensing Drivers of agricultural change Citizen science Climate Hydrology and watersheds Numerous common themes that emerged from the workshop include the following: The vital importance of completing foundational environmental datasets that are nationally consistent and are essential to multiple sectors, such as the Soil Survey Geographic database high-resolution soils data, a minimum 5-meter resolution digital elevation model, national hydrographic data, high-resolution land cover data, time series high-resolution spatial climate data from historical to future time steps, and a national wetland inventory. Improved, nationally consistent environmental datasets (integrated with targeted observations) will dramatically advance forecasting capacity and support early warning systems (that is, drought, forest disease); however, multiagency coordination should focus on decision support tools that convey appropriate actions and responses to adapt to, and mitigate, potential negative consequences. Digitizing and providing access to the vast stores of underused historical data that can be leveraged for this purpose is of national importance. Modern computational techniques and the ever-increasing flow of environmental data from ground and remote observations can support improved understanding of environmental change. Success of understanding patterns of change for decision making requires establishing baselines from which change can be measured. The value of digitized historical data is greater than ever before. There is a need to recognize the multifaceted potential of citizen science to engage the public in resource stewardship, to create the next generation of science, technology, engineering, math, and environmental leaders, and to have sufficient field personnel to monitor environmental trends, including early detection of alien invasive species, phenological shifts, shifting distribution and abundance of indicator species, and species inventories. The Federal government has an essential role in creating the infrastructure to dramatically improve mobilization of citizen science (and other) data by fostering the following: creation of data standards, creation of nationally consistent framework datasets, vertical integration of observation data, visualization and dissemination of aggregated datasets, and calculation and communication of derived trends. Current and near future trends in the availability of remotely sensed data (rapid expansion of satellite fleets and drones) is revolutionizing access to near-real-time ecological data. Targeted integration with ground-based observations and instrumentation has an extremely valuable role in validating remotely sensed data, filling data gaps, improving data quality, and fully realizing the potential of the near-real-time monitoring of environmental indicator trends. Integrated management of environmental data at the landscape scale is required even as specific actions on the ground are largely local in nature. The workshop highlighted numerous success stories; however, almost every breakout group pointed out the still-too-fragmented nature of the current data landscape. Management and delivery of the necessary data, tools, and analyses to sustain our Nation’s environmental capital must be a collaborative effort between Federal, State, and local governments, academia, nonprofits, and the commercial sector, even though the responsibilities of each sector are different.

Open-File Report↗

Factors affecting nutrient trends in major rivers of the Chesapeake Bay Watershed

Trends in nutrient loads and flow-adjusted concentrations in the major rivers entering Chesapeake Bay were computed on the basis of water-quality data collected between 1985 and 1998 at 29 monitoring stations in the Susquehanna, Potomac, James, Rappahannock, York, Patuxent, and Choptank River Basins. Two computer models-the Chesapeake Bay Watershed Model (WSM) and the U.S. Geological Survey's 'Spatially Referenced Regressions on Watershed attributes' (SPARROW) Model-were used to help explain the major factors affecting the trends. Results from WSM simulations provided information on temporal changes in contributions from major nutrient sources, and results from SPARROW model simulations provided spatial detail on the distribution of nutrient yields in these basins. Additional data on nutrient sources, basin characteristics, implementation of management practices, and ground-water inputs to surface water were analyzed to help explain the trends. The major factors affecting the trends were changes in nutrient sources and natural variations in streamflow. The dominant source of nitrogen and phosphorus from 1985 to 1998 in six of the seven tributary basins to Chesapeake Bay was determined to be agriculture. Because of the predominance of agricultural inputs, changes in agricultural nutrient sources such as manure and fertilizer, combined with decreases in agricultural acreage and implementation of best management practices (BMPs), had the greatest impact on the trends in flow-adjusted nutrient concentrations. Urban acreage and population, however, were noted to be increasing throughout the Chesapeake Bay Watershed, and as a result, delivered loads of nutrients from urban areas increased during the study period. Overall, agricultural nutrient management, in combination with load decreases from point sources due to facility upgrades and the phosphate detergent ban, led to downward trends in flow-adjusted nutrient concentrations atmany of the monitoring stations in the watershed. The loads of nutrients, however, were not reduced significantly at most of the monitoring stations. This is due primarily to higher streamflow in the latter years of the monitoring period, which led to higher loading in those years. Results of this study indicate a need for more detailed information on BMP effectiveness under a full range of hydrologic conditions and in different areas of the watershed; an internally consistent fertilizer data set; greater consideration of the effects of watershed processes on nutrient transport; a refinement of current modeling efforts; and an expansion of the non-tidal monitoring network in the Chesapeake Bay Watershed.

Delaware, Maryland, New York, Pennsylvania, Virgin↗

Large streamflow differences between forested and urbanized watersheds in the energy-limited eastern United States: The role of evapotranspiration and impervious surfaces

Urban forests and other green infrastructures have been viewed as part of the “Nature-based Solutions” (NbS) to mitigate emerging urban environmental change. This study focuses on the role of evapotranspiration (ET) in regulating water balances of small watersheds in the eastern United States. We compared streamflow and ET patterns at daily, monthly and annual scales and linked these hydrological variables to the physical properties of 11 paired watersheds dominated by forests (FW) or urban (UW) land covers. The annual precipitation ranged from 1028 mm to 1683 mm and potential ET (PET) from 815 mm to 1450 mm. The mean annual flow/precipitation (Q/P) ratios were 0.26 ± 0.13 and 0.41 ± 0.1 for FW and UW, respectively. Overall, UW had lower annual ET (772 mm in UW vs. 947 mm in FW), but higher mean annual and (∼58% higher), monthly water yield (17%–186% higher), and peakflow rates (up to 100 times higher) than FW. The streamflow differences between FW and UW were most pronounced during the growing season and early winter (June-November). The mean Q/P ratios for 30 large hurricane events (2016–2021) were 0.12 ± 0.11 and 0.38 ± 0.23 for FW and UW, respectively. The flow rates in the dormant season (around December-May) in UW were similar or lower than FW. We developed conceptual models to explain the seasonal and storm event streamflow differences using background climate (PET), ET, and land surface characteristics. Urban NbS designs should factor in strategies that maximize ET while minimizing impervious surfaces enhancing watershed “sponge” and “pump” functions.

eastern United States↗

2005 annual progress report: Elk and bison grazing ecology in the Great Sand Dunes complex of lands

In 2000 the U.S. Congress authorized the expansion of the former Great Sand Dunes National Monument by establishing a new Great Sand Dunes National Park and Preserve in its place, and establishing the Baca National Wildlife Refuge. The establishment of Great Sand Dunes National Park and Preserve and the new Baca National Wildlife Refuge in the San Luis Valley (SLV), Colorado was one of the most significant land conservation actions in the western U.S. in recent years. The action was a result of cooperation between the National Park Service (NPS), U.S. Fish and Wildlife Service (USFWS), Bureau of Land Management (BLM), U.S. Forest Service (USDA-FS), and The Nature Conservancy (TNC). The new national park, when fully implemented, will consist of 107,265 acres, the new national preserve 41,872 acres, and the new national wildlife refuge (USFWS lands) 92,180 acres (fig. 1). The area encompassed by this designation protects a number of natural wonders and features including a unique ecosystem of natural sand dunes, the entire watershed of surface and groundwaters that are necessary to preserve and recharge the dunes and adjacent wetlands, a unique stunted forest, and other valuable riparian vegetation communities that support a host of associated wildlife and bird species. When the National Park was initially established, there were concerns about overconcentrations and impacts on native plant communities of the unhunted segments of a large and possibly growing elk (Cervus elaphus) population. This led to the designation of the Preserve as a compromise solution, where the elk could be harvested. The Preserve Unit, however, will not address all the ungulate management challenges. In order to reduce the current elk population, harvests of elk may need to be aggressive. But aggressive special hunts of elk to achieve population reductions can result in elk avoidance of certain areas or elk seeking refuge in areas where they cannot be hunted, while removals of whole herd segments and abandonment or alterations of migration routes can occur (Smith and Robbins, 1994; Boyce and others, 1991). Elk may seek refuge from hunting in the newly expanded Park Unit and TNC lands where they might overconcentrate and impact unique vegetation communities. In these sites of refugia, or preferred loafing sites, elk and bison could accelerate a decline in woody riparian shrubs and trees. This decline may also be due to changes in hydrology, climatic, or dunal processes, but ungulate herbivory might exacerbate the effects of those processes. To address the questions and needs of local resource managers, a multi-agency research project was initiated in 2005 to study the ecology, forage relations, and habitat relations of elk and bison in the Great Sand Dunes–Sangre de Cristo–Baca complex of lands. Meetings and discussions of what this research should include were started in 2001 with representatives from NPS, USFWS, TNC, the Colorado Division of Wildlife (CDOW), and USDA-FS/BLM. The final study plan was successfully funded in 2004 with research scheduled to start in 2005. The research was designed to encompass three major study elements: (1) animal movements and population dynamics, (2) vegetation and nutrient effects from ungulate herbivory, and (3) development of ecological models, using empirical data collected from the first two components, that will include estimates of elk carrying capacity and management scenarios for resource managers.

Colorado↗

Potentiometric surfaces (2013, 2015), groundwater quality (2010–15), and water-level changes (2011–13, 2013–15) in the Sparta-Memphis aquifer in Arkansas

The Sparta-Memphis aquifer, present across much of eastern Arkansas, is the second most used groundwater resource in the State, with the Mississippi River Valley alluvial aquifer being the primary groundwater resource. The U.S. Geological Survey, in cooperation with Arkansas Department of Agriculture-Natural Resources Division, Arkansas Geological Survey, Natural Resources Conservation Service, Union County Water Conservation Board, and the Union County Conservation District, collects groundwater data across the Sparta-Memphis aquifer extent in Arkansas. This report presents water-level data for measurements conducted during two time periods, January–May 2013 and January–June 2015, and discusses water-level altitude changes for the 2011–13 and 2013–15 periods in the Sparta-Memphis aquifer. Accompanying water-level data in this report include groundwater-quality data for the period 2010–15 in the Sparta-Memphis aquifer. Groundwater data can guide ongoing and future groundwater-monitoring efforts and inform management of the aquifers in Arkansas. Water levels measured at 306 wells from January to May 2013 and 273 wells from January to June 2015 are graphically presented as potentiometric-surface maps. Measurements from 2011, 2013, and 2015 were used in the construction of 2011–13 and 2013–15 water-level change maps. Select long-term hydrographs are included in the report to illustrate water-level changes at the local scale. Water-level data show the influence of climate, pumping, and conservation and management efforts on groundwater levels. With respect to climate, the study area experienced extreme drought conditions between January 2011 and December 2012. The proximate effects of drought—increased evapotranspiration, decreased recharge, and increased irrigation needs—resulted in water-level declines that were particularly notable in the northern and central portions of the study area. Groundwater sampled in 2010–15 from 148 wells completed in the Sparta-Memphis aquifer was analyzed for specific conductance, pH, chloride (Cl) concentration, and bromide (Br) concentration. In 2015, groundwater-quality data from 103 wells completed in the Sparta-Memphis aquifer had a median specific conductance of 356 microsiemens per centimeter at 25 degrees Celsius and a median Cl concentration of 9.5 milligrams per liter (mg/L). The data show two areas of higher Cl (greater than 10 mg/L) and higher Br (greater than 0.5 mg/L) concentrations in Union, Calhoun, and Bradley Counties in southern Arkansas and Monroe and Phillips Counties in eastern-central Arkansas. A Cl and Br mixing model indicates the two regions of wells may have different sources of higher salinity. In the greater Union County area, water in most wells may be a mixture of recharge or precipitation and higher salinity groundwater from the Nacatoch aquifer. Water in wells in eastern-central Arkansas may be sourced from aquifers having a higher Cl concentration (and thus, also a higher Cl-to-Br ratio).

Arkansas↗

Movement and habitat use of sika and white-tailed deer on Assateague Island National Seashore, Maryland

This research project was conducted to describe habitat use of sika deer ( Cervus nippon ) and white-tailed deer ( Odocoileus virginianus ) and possibly attribute the effects of ungulate herbivory to specific deer species, if spatial separation in habitat use could be identified. Sturm (2007) conducted an exclosure study to document the effect of feral horse ( Equus caballus ) herbivory, deer herbivory, and horse and deer herbivory combined on plant communities. Sturm (2007) found that ungulate herbivory reduced plant species richness, evenness, and diversity in the maritime forest and affected species composition in all habitats studied. Sturm (2007) also found that herbivory on some species could be directly attributable to either horse or deer. However, the effects of sika and white-tailed deer herbivory could not be separated via an exclosure study design because of the difficulty of passively excluding one deer species but not the other. We captured white-tailed deer and sika deer in January–March of 2006 and 2007 throughout the Maryland portion of Assateague Island. Deer were fitted with radio-collars and their survival and locations monitored via ground telemetry. Up to four locations were acquired per deer each week during early (May–June) and late (August–September) growth periods for vegetation on the island. Also, we estimated deer locations during a dormant vegetation period (November– December 2006). We used these data to estimate survival and harvest rates, document movements, and model habitat use. We captured and fitted 50 deer with radio-collars over the course of the study. Of these 50 deer, 36 were sika and 14 were white-tailed deer. Of the 36 sika deer, 10 were harvested, three were likely killed by hunters but not recovered, and one died of natural causes while giving birth. Of the 14 white-tailed deer, three were harvested, one was illegally killed, and two were censored because of study-related mortality. Annual survival was 0.48 (95% CI = 0.16–0.82) for male white-tailed deer, 0.74 (95% CI = 0.44–0.91) for female white-tailed deer, 0.56 (95% CI = 0.35–0.75) for male sika deer, and 0.86 (95% CI = 0.70–0.94) for female sika deer. The harvest rate was 0.12 (95% CI = 0.04–0.27) for female sika deer, 0.44 (95% CI = 0.25–0.65) for male sika deer, 0.18 (95% CI = 0.05–0.51) for female white-tailed deer, and 0.38 (95% CI = 0.10–0.78) for male white-tailed deer. Annual survival rates for both species were similar to what has been observed in other populations. Unfortunately, small sample sizes for male white-tailed deer limited inferences about harvest and survival rates, but harvest rates of females for both species were similar to other published studies. Hunting was the primary cause of mortality, and outside the hunting season survival was 0.98–1.00 for all species and sexes. We found that the home range area of sika deer was much greater than the home range area of white-tailed deer, but failed to detect any difference between sexes or among seasons. Sika deer also made long-distance movements and left the Maryland portion of Assateague Island. No sika deer left Assateague island during our study, but we did document the dispersal of a male whitetailed deer to the mainland. In their native range, sika deer have been able to readily expand populations and occupy vacant habitat (Kaji et al. 2000; Kaji et al. 2004). The long distance movements we observed on Assateague Island, especially relative to white-tailed deer, may reflect the ability of this species to exploit food resources that may be limited in quality or quantity, or both. However, we did not collect data to assess use of food resources by sika deer and whether this may have influenced long distance movements. We found both species of deer were less likely to use a habitat the further it was located from cover, which was defined as tall shrub or forest vegetation. For every 10 m (32 ft) from cover each species of deer was 1.23–1.38 times less likely to use any given habitat. Patterns in use of vegetation classes were similar across species and seasons. Relative to forest habitat, both species avoided dune herbaceous, disturbed lands, sand, and water categories. Both species neither avoided nor preferred developed herbaceous, low shrub, marsh herbaceous, and tall shrub categories compared to the forest category. However, there were consistent differences between the two species. During spring, white-tailed deer were more likely than sika deer to use forested, tall shrub, disturbed herbaceous, and sand areas, but were less likely to use all other habitats. During summer, habitat use was similar between the two species except that white-tailed deer tended to use forested habitat more. During winter, white-tailed deer were less likely to use dune herbaceous, low shrub, and forested habitats than sika deer. Sturm (2007) identified differential browsing on plant species between horses and deer, but his experimental design did not permit detection of differential browsing between sika and whitetailed deer. Our study of habitat use did not provide information to identify plant species that may be differentially consumed by sika and white-tailed deer based on differences in habitat use. We envision two approaches to addressing the effects of deer browsing. One approach would be further research that identifies the food habits of both deer species at the plant species level. This would be similar to food habits research conducted by Keiper (1985) and others or could involve direct observation of food consumption by both species. However, both fecal analysis and direct observation would be time-consuming and not guaranteed to identify differences. If the goal of ungulate population management is to protect the island ecosystem, another approach involving manipulation of deer abundance and monitoring the response of plant species known to be preferentially consumed by deer would be a more direct method of assessing effects of deer herbivory (Sturm 2007). Moreover, such an approach is not predicated on detecting differences between deer species. Direct manipulation of deer abundance could be incorporated into an adaptive management program (Williams et al. 2007) and may provide greater benefits to the management of ASIS in the long term. Harvest management decisions for white-tailed deer and sika deer are made on an ongoing basis and by coupling these decisions with a vegetative monitoring program it may be possible to reduce or minimize adverse effects of ungulate herbivory. Furthermore, management of feral horses could be incorporated into the decision process.

Maryland, Virginia↗

Genome resequencing clarifies phylogeny and reveals patterns of selection in the toxicogenomics model Pimephales promelas

Background The fathead minnow ( Pimephales promelas ) is a model species for toxicological research. A high-quality genome reference sequence is available, and genomic methods are increasingly used in toxicological studies of the species. However, phylogenetic relationships within the genus remain incompletely known and little population-genomic data are available for fathead minnow despite the potential effects of genetic background on toxicological responses. On the other hand, a wealth of extant samples is stored in museum collections that in principle allow fine-scale analysis of contemporary and historical genetic variation. Methods Here we use short-read shotgun resequencing to investigate sequence variation among and within Pimephales species. At the genus level, our objectives were to resolve phylogenetic relationships and identify genes with signatures of positive diversifying selection. At the species level, our objective was to evaluate the utility of archived-sample resequencing for detecting selective sweeps within fathead minnow, applied to a population introduced to the San Juan River of the southwestern United States sometime prior to 1950. Results We recovered well-supported but discordant phylogenetic topologies for nuclear and mitochondrial sequences that we hypothesize arose from mitochondrial transfer among species. The nuclear tree supported bluntnose minnow ( P. notatus ) as sister to fathead minnow, with the slim minnow ( P. tenellus ) and bullhead minnow ( P. vigilax ) more closely related to each other. Using multiple methods, we identified 11 genes that have diversified under positive selection within the genus. Within the San Juan River population, we identified selective-sweep regions overlapping several sets of related genes, including both genes that encode the giant sarcomere protein titin and the two genes encoding the MTORC1 complex, a key metabolic regulator. We also observed elevated polymorphism and reduced differentation among populations (F ST ) in genomic regions containing certain immune-gene clusters, similar to what has been reported in other taxa. Collectively, our data clarify evolutionary relationships and selective pressures within the genus and establish museum archives as a fruitful resource for characterizing genomic variation. We anticipate that large-scale resequencing will enable the detection of genetic variants associated with environmental toxicants such as heavy metals, high salinity, estrogens, and agrichemicals, which could be exploited as efficient biomarkers of exposure in natural populations.

PeerJ↗

Mute swans: Natural (?) environmental indicators

The rapid expansion of the Chesapeake Bay's population of feral mute swans (Cygnus olar), coupled with a dramatic Bay-wide decline in submerged aquatic vegetation (SAV), has fueled much of the current debate surrounding the need for a management plan to protect the aquatic food resources that are critical to many species native to the Bay. Crucial to this decision process is a sound understanding of the ecological ramifications of having the year-round presence of a large, nonnative, aquatic herbivore on the Bay. Ultimately, this will require a quantitative assessment of the ecological harm currently posed by mute swans before a biologically defensible management strategy can be developed. Unfortunately, very little new information specific to the Bay's mute swan population has been gathered since Reese first studied them in the late 1960s and 1970s. While the debate over what to do about the rapidly expanding mute swan population continues, there is much that can be gained from study of this beautiful intruder. Several recent studies of the feeding habits of mute swans have shown that mutes can provide a unique barometer, or indicator, of environmental conditions. Because of their reliance on SAV as a primary food source, monitoring the density of swans utilizing a particular area can give some indication of the status of the area's grass beds. This phenomenon was clearly demonstrated during the summer of 1999 when there was a dramatic decline in the number of swans observed around the Eastern Neck NWR, a traditional population stronghold. The shift in bird use was precipitated by a rapid, large-scale collapse of the area's aquatic grass beds, possibly the result of a prolonged drought. During the winter of 2000/2001, a similar ecological assessment was conducted by comparing body weights of swans collected from Tangier Sound, an area with relatively abundant grass beds, and swans from the waters adjacent to Eastern Neck Island. Swans weights tended to reflect the conditions of their surroundings, with the Tangier Sound birds being slightly heavier at the onset of the breeding season. Interestingly, the birds at Eastern Neck showed a 1 kg decline in weight after dispersal from their wintering locations and entered the breeding season with noticeably less subcutaneous fat than the birds sampled in Tangier Sound. The fact that mute swans are nonmigratory and feed exclusively on benthic food items makes them an ideal organ-ism to monitor the degree of contamination of sediments within the Bay. In 1995, we compared the accumulation of metals by mute swans and other waterfowl and related it to metal concentrations in the sediments from the areas where the birds were collected. This study led to the development of an exposure model that more accurately assesses the risk of exposure to environmental contaminants through incidental ingestion of sediments, as opposed to the traditional assessment of contaminant accumulation through the food chain. This sediment exposure pathway was subsequently shown to be the primary route of exposure of swans to metals in risk assessments conducted at two Superfund sites.

Book chapter↗

Ground-water contamination at Wurtsmith Air Force Base, Michigan

A sand and gravel aquifer of glacial origin underlies Wurtsmith Air Force Base in northeastern lower Michigan. The aquifer overlies a thick clay layer at an average depth of 65 feet. The water table is about 10 feet below land surface in the western part of the Base and about 25 feet below land surface in the eastern part. A ground-water divide cuts diagonally across the Base from northwest to southeast. South of the divide, ground water flows to the Au Sable River; north of the divide, it flows to Van Etten Creek and Van Etten Lake. Mathematical models were used to aid in calculating rates of groundwater flow. Rates range from about 0.8 feet per day in the eastern part of the Base to about 0.3 feet per day in the western part. Models also were used as an aid in making decisions regarding purging of contaminated water from the aquifer. In 1977, trichloroethylene was detected in the Air Force Base water-supply system. It had leaked from a buried storage tank near Building 43 in the southeastern part of the Base and moved northeastward under the influence of the natural ground-water gradient and the pumping of Base water-supply wells. In the most highly contaminated part of the plume, concentrations are greater than 1,000 micrograms per liter. Current purge pumping is removing some of the trichloroethylene, and seems to have arrested its eastward movement. Pumping of additional purge wells could increase the rate of removal. Trichloroethylene has also been detected in ground water in the vicinity of the Base alert apron, where a plume from an unknown source extends northeastward off Base. A smaller, less well-defined area of contamination also occurs just north of the larger plume. Trichloroethylene, identified near the waste-treatment plant, seepage lagoons, and the northern landfill area, is related to activities and operations in these areas. Dichloroethylene and trichloroethylene occur in significant quantities westward of Building 43, upgradient from the major spill site. Benzene, indicative of ground-water contamination by a fuel substance, occurs in an area northeast of the bulk-fuel storage area. Analysis of a variety of chemical, physical, and biologic characteristics of water on the Base indicate that there is a measurable affect on ground-water quality from landfills, the seepage lagoon, and the waste-treatment plant.

Michigan↗

Delineation of marsh types of the Texas coast from Corpus Christi Bay to the Sabine River in 2010

Coastal zone managers and researchers often require detailed information regarding emergent marsh vegetation types for modeling habitat capacities and needs of marsh-reliant wildlife (such as waterfowl and alligator). Detailed information on the extent and distribution of marsh vegetation zones throughout the Texas coast has been historically unavailable. In response, the U.S. Geological Survey, in cooperation and collaboration with the U.S. Fish and Wildlife Service via the Gulf Coast Joint Venture, Texas A&M University-Kingsville, the University of Louisiana-Lafayette, and Ducks Unlimited, Inc., has produced a classification of marsh vegetation types along the middle and upper Texas coast from Corpus Christi Bay to the Sabine River. This study incorporates approximately 1,000 ground reference locations collected via helicopter surveys in coastal marsh areas and about 2,000 supplemental locations from fresh marsh, water, and “other” (that is, nonmarsh) areas. About two-thirds of these data were used for training, and about one-third were used for assessing accuracy. Decision-tree analyses using Rulequest See5 were used to classify emergent marsh vegetation types by using these data, multitemporal satellite-based multispectral imagery from 2009 to 2011, a bare-earth digital elevation model (DEM) based on airborne light detection and ranging (lidar), alternative contemporary land cover classifications, and other spatially explicit variables believed to be important for delineating the extent and distribution of marsh vegetation communities. Image objects were generated from segmentation of high-resolution airborne imagery acquired in 2010 and were used to refine the classification. The classification is dated 2010 because the year is both the midpoint of the multitemporal satellite-based imagery (2009–11) classified and the date of the high-resolution airborne imagery that was used to develop image objects. Overall accuracy corrected for bias (accuracy estimate incorporates true marginal proportions) was 91 percent (95 percent confidence interval [CI]: 89.2–92.8), with a kappa statistic of 0.79 (95 percent CI: 0.77–0.81). The classification performed best for saline marsh (user’s accuracy 81.5 percent; producer’s accuracy corrected for bias 62.9 percent) but showed a lesser ability to discriminate intermediate marsh (user’s accuracy 47.7 percent; producer’s accuracy corrected for bias 49.5 percent). Because of confusion in intermediate and brackish marsh classes, an alternative classification containing only three marsh types was created in which intermediate and brackish marshes were combined into a single class. Image objects were reattributed by using this alternative three-marsh-type classification. Overall accuracy, corrected for bias, of this more general classification was 92.4 percent (95 percent CI: 90.7–94.2), and the kappa statistic was 0.83 (95 percent CI: 0.81–0.85). Mean user’s accuracy for marshes within the four-marsh-type and three-marsh-type classifications was 65.4 percent and 75.6 percent, respectively, whereas mean producer’s accuracy was 56.7 percent and 65.1 percent, respectively. This study provides a more objective and repeatable method for classifying marsh types of the middle and upper Texas coast at an extent and greater level of detail than previously available for the study area. The seamless classification produced through this work is now available to help State agencies (such as the Texas Parks and Wildlife Department) and landscape-scale conservation partnerships (such as the Gulf Coast Prairie Landscape Conservation Cooperative and the Gulf Coast Joint Venture) to develop and (or) refine conservation plans targeting priority natural resources. Moreover, these data may improve projections of landscape change and serve as a baseline for monitoring future changes resulting from chronic and episodic stressors.

Texas↗

Geochemical processes and the effects of natural organic solutes on the solubility of selenium in coal-mine backfill samples from the Powder River basin, Wyoming

Geochemical processes and the effects of natural organic solutes on the solubility of selenium in coal-mine backfill aquifers were investigated. Backfill and ground-water samples were collected at coal mines in the Powder River Basin, Wyoming. Backfill was generally dominated by aluminum (14,400 to 49,000 mg/kg (milligrams per kilogram)), iron (3,330 to 23,200 mg/kg), and potassium (7,950 to 18,000 mg/kg). Backfill saturated-paste selenium concentrations ranged from 1 to 156 mg/kg (microsiemens per kilogram). Ground-water total selenium concentrations ranged from 3 to 125 mg/L. Dissolved organic carbon in all ground-water samples was dominated by hydrophobic and hydrophilic acids (38 to 84 percent). Selenite sorption/desorption experiments were conducted using background solutions of distilled-deionized water, 0.1 molar calcium chloride, and isolated hydrophobic and hydrophilic acids. Selenite sorption was larger when 0.1 molar calcium chloride was used. The addition of hydrophilic acid decreased selenite sorption more than the addition of hydrophobic acids. Geochemical modelling was used to predict the solid phases controlling dissolved selenium concentrations and to evaluate the effects of dissolved organic carbon on selenium solubility. Results suggested that 55 to 90 percent of selenium in backfill precipitation/dissolution extracts was dominated by magnesium selenate ion pairs. Dissolved organic carbon had little effect on selenium speciation. A redox chamber was constructed to control Eh and pH in water and backfill-core sample suspensions. The response of selenite and selenate in water samples to redox conditions did not follow thermodynamic predictions. Reduction of selenate in water samples did not occur at any of the redox levels tested.

Water-Resources Investigations Report↗

Fire rehabilitation effectiveness: a chronosequence approach for the Great Basin

Federal land management agencies have invested heavily in seeding vegetation for emergency stabilization and rehabilitation (ES&R) of non-forested lands. ES&R projects are implemented to reduce post-fire dominance of non-native annual grasses, minimize probability of recurrent fire, quickly recover lost habitat for sensitive species, and ultimately result in plant communities with desirable characteristics including resistance to invasive species and resilience or ability to recover following disturbance. Land managers lack scientific evidence to verify whether seeding non-forested lands achieves their desired long-term ES&R objectives. The overall objective of our investigation is to determine if ES&R projects increase perennial plant cover, improve community composition, decrease invasive annual plant cover and result in a more desirable fuel structure relative to no treatment following fires while potentially providing habitat for Greater Sage-Grouse, a species of management concern. In addition, we provide the locations and baseline vegetation data for further studies relating to ES&R project impacts. We examined effects of seeding treatments (drill and broadcast) vs. no seeding on biotic and abiotic (bare ground and litter) variables for the dominant climate regimes and ecological types within the Great Basin. We attempted to determine seeding effectiveness to provide desired plant species cover while restricting non-native annual grass cover relative to post-treatment precipitation, post-treatment grazing level and time-since-seeding. Seedings were randomly sampled from all known post-fire seedings that occurred in the four-state area of Idaho, Nevada, Oregon and Utah. Sampling locations were stratified by major land resource area, precipitation, and loam-dominated soils to ensure an adequate spread of locations to provide inference of our findings to similar lands throughout the Great Basin. Nearly 100 sites were located that contained an ES&R project. Of these sites, 61 were seeded by using a drill, 27 were broadcast aerially, and 12 had a combination of both. We randomly sampled three burned and seeded, burned and unseeded, and unburned and unseeded locations in the vicinity of the fire, each within the same ecological site. We measured foliar cover of all plant functional groups (perennial or annual, shrub, grass, forb, native or introduced), biological soil crusts, and abiotic (bare soil and litter) variables using the line-point intercept protocol. Fuel loads and horizontal fuel continuity were measured. We applied linear mixed models to response variables (cover and density of plant groups) relative to the dependent variables (seeding treatments and precipitation/temperature relationships. Post-fire strengths with native perennial grasses or shrubs in mixes did not increase density or cover of these groups significantly relative to unseeded, burned areas. Seeded non-native perennial grasses and the shrub Bassia prostrata were effective in providing more cover in aerial and drill seedings. Seeded non-native perennial grass cover increased with increased annual precipitation regardless of seeding type. Seeding native shrubs, particularly Artemisia tridentata, did not significantly increase shrub cover in burned areas. Cover of undesirable non-native annual grasses was lower in drill seedings relative to unseeded areas but only at higher elevations. Seeding effectiveness after wildfire is unpredictable in drier, low elevation environments, and our findings indicate management objectives are more likely met when focusing efforts on higher elevation or higher precipitation locations where establishment of perennial grasses is more likely. On sites where potential for invasion and dominance of non-native annuals is high, such as lower and drier sites, intensive methods of restoration that include invasive plant control before seeding may be required. Where establishment of native perennial plants is the goal, managers might consider using native-only seed mixtures, because we found that the non-native perennials typically used in Great Basin restoration efforts are selected for their competitive nature and may reduce establishment of less competitive native species. Although we attempted to include information on livestock grazing history after seedings, we were unable to extract sufficient data from files to address this topic that may play an additional role in understanding native plant abundance post-fire seeding. Evaluation of drill and aerial seeding effects on fuel characteristics focused on two metrics that are standard inputs for fire behavior models, fuel load and fuel continuity. Fuel loads were evaluated separately for total fuel load biomass, and the individual components that sum to total biomass, namely herbaceous, shrub, shrub:herbaceous ratio, litter, 10-hour, and 100-hour fuel biomasses. Fuel continuity was evaluated using the following cover categories, total, annual grass, annual forb, perennial forb perennial grass, shrub, litter, vegetative interspace, and perennial interspace. Drill seeding did not affect fuel loads, except to reduce 10-hour fuels, probably due to mechanical destruction of dead and down fuels by the drill seeding equipment. Drill seeding did affect fuel continuity, specifically decreasing total plant cover by increasing perennial grass cover which suppressed annual grass and litter production resulting in a net decrease in continuity, but only at the elevations above approximately 1500m. Aerial seeding had no effect on any fuel load or fuel continuity category. For the Greater Sage-Grouse habitat study, we developed multi-scale empirical models of sage-grouse occupancy in 211 randomly located plots within a 40 million ha portion of the species’ range. We then used these models to predict sage-grouse habitat quality at 101 ES&R seeding projects. We compared conditions at restoration sites to published habitat guidelines. Sage-grouse occupancy was positively related to plot- and landscape-level dwarf sagebrush (Artemisia arbuscula, A. nova, A. tripartita) and big sagebrush steppe, and negatively associated with non-native grass and human development. The predicted probability of sage-grouse occupancy at treated plots was low on average (0.07–0.09) and was not significantly different from burned areas that had not been treated. Restoration was more often successful at higher elevation sites with low annual temperatures, high spring precipitation, and high plant diversity. No plots seeded after fire (n=313) met all overstory guidelines for breeding habitats, but approximately 50% met understory guidelines, particularly for perennial grasses. This trend was similar for summer habitat. Ninety-eight percent of treated plots did not meet winter habitat guidelines. Restoration actions in burned areas did not increase the probability of meeting most guideline criteria. The probability of meeting guidelines was influenced by a latitudinal gradient, local climate, and topography. Post-fire seeding treatments in Great Basin sagebrush shrublands generally have not created high quality habitat for sage-grouse. Understory conditions are more likely to be adequate than those of overstory, but in unfavorable climates, establishing forbs and reducing cheatgrass dominance is unlikely. Reestablishing sagebrush cover will require more than 20 years using the restoration methods of the past two decades. Given current fire frequencies and restoration capabilities, protection of landscapes containing a mix of dwarf sagebrush and big sagebrush steppe, minimal human development, and low non-native plant cover may provide the best opportunity for conservation of sage-grouse habitats. Our database of ES&R locations has used the Land Treatment Digital Library to archive data and location information regarding our study (see Pilliod and Welty 2013). This has contributed to two additional studies. One examined the potential spread of Bassia prostrata (aka Kochia prostrata; forage kochia) from ES&R project locations (Gray and Muir 2013). The second used remote sensing to determine the phenology of vegetation green-up on post-fire seeded sites (Sankey et al. 2013).

California;Idaho;Oregon;Utah↗

Use of borehole and surface geophysics to investigate ground-water quality near a road-deicing salt-storage facility, Valparaiso, Indiana

Borehole and surface geophysics were used to investigate ground-water quality affected by a road-deicing salt-storage facility located near a public water-supply well field. From 1994 through 1998, borehole geophysical logs were made in an existing network of monitoring wells completed near the bottom of a thick sand aquifer. Logs of natural gamma activity indicated a uniform and negligible contribution of clay to the electromagnetic conductivity of the aquifer so that the logs of electromagnetic conductivity primarily measured the amount of dissolved solids in the ground water near the wells. Electromagnetic-conductivity data indicated the presence of a saltwater plume near the bottom of the aquifer. Increases in electromagnetic conductivity, observed from sequential logging of wells, indicated the saltwater plume had moved north about 60 to 100 feet per year between 1994 and 1998. These rates were consistent with estimates of horizontal ground-water flow based on velocity calculations made with hydrologic data from the study area. Ratios of chloride to bromide concentrations in water samples were used to distinguish sources of chloride in the ground water?whether from road-deicing salt, domestic wastewater, or natural occurrences. Water samples identified with the chloride/bromide ratios as being affected by road-deicing salt had concentrations of dissolved solids, chloride, and sodium many times the background levels for the study area. The largest concentrations were in water from wells near the salt-storage facility?12,400 to 12,800 milligrams per liter (mg/L) dissolved solids, 6,730 to 7,230 mg/L chloride, and 3,690 to 4,400 mg/L sodium. A conceptual, multi-layer model was developed to describe the vertical extent of the saltwater plume in the vicinity of the monitoring wells. A relation was derived between average borehole electromagnetic conductivity in the screened interval of the wells in the saltwater plume and concentrations of dissolved solids in water samples from those wells. This relation was used in the model to show borehole electromagnetic conductivity in transects of wells as a zone of saline water overlain by zones of brackish water and freshwater. The thickness and altitude of the zones of saline and brackish water decreased with increased distance from the salt-storage facility. Two surface surveys of terrain electromagnetic conductivity were used to map the horizontal extent of the saltwater plume in areas without monitoring wells. Background values of terrain conductivity were measured in an area where water-quality and borehole geophysical data did not indicate saline or brackish water. Based on a guideline from previous case studies, the boundaries of the saltwater plume were mapped where terrain conductivity was 1.5 times background. The extent of the saltwater plume, based on terrain conductivity, generally was consistent with the available water-quality and borehole electromagnetic-conductivity data and with directions of ground-water flow determined from water-level altitudes.

Indiana↗

Monitoring the water-quality response of agricultural conservation practices in the Bucks Branch watershed, Sussex County, Delaware, 2014–16

The purpose of this study was to evaluate the effects of irrigation and cover crops as conservation practices on water quality in groundwater and streams. Bucks Branch, a stream in the Nanticoke River watershed in southwestern Delaware, was identified as having one of the highest concentrations of nitrate in all surface-water sites sampled by the Delaware Department of Natural Resources and Environmental Control (DNREC). The study site is on two adjacent fields bordering Bucks Branch, one that has used irrigation since 2000 and one with dryland farming; both under conservation tillage and long-term rotation of corn, soybean, and small grain crops. A streamgage was installed near the study site fields to measure streamflow and water quality. The study area is typical of farming practices and environmental conditions throughout much of the intensively farmed agricultural land of the Coastal Plain of Delaware and surrounding parts of Maryland. Monitoring was conducted from January 2014 through June 2016. Corn was grown on both fields during the two growing seasons of the study period, and cover crops were planted before or shortly after harvest on both fields. During the second year of data collection, the effects of radish and rye grass cover crops on nutrient transport were studied. The combined results from data collected for this study show that water and nitrate moved below the root zone year round when soil moisture was high, especially after significant rainfall and frequently after irrigation. Soil water sampled 2 to 3 weeks after nutrients were applied had nitrate concentrations greater than 50 milligrams per liter as nitrogen (mg/L as N) and may be a significant source of nitrate to groundwater. Whereas recharge containing elevated nitrate concentrations also occurred under the dryland field, it was less frequent and of lower concentration than recharge under the irrigated field. Nitrate was present in all groundwater samples from these sites. Groundwater estimated to have recharged within 10 years or less had higher median concentrations of nitrate than in older water samples. The oldest groundwater encountered was over 30 years old, and had traveled along the longest, deep flowpaths from upland fields to the stream. The median nitrate concentration was 18 mg/L as N in younger water (less than 10 years old) beneath the irrigated field, compared to about 10 mg/L as N in younger water beneath the dryland field. Samples from the shallow upland wells in both study fields showed little, if any, evidence of denitrification. Several samples from deeper wells and from wells near forested riparian zone wetlands that border both fields did show partial denitrification. A mixing model estimated that between 12 and 22 percent of the nitrate discharging to the stream was lost through uptake and denitrification upstream of the streamgage on Bucks Branch. Continuous data collected at this site and evidence of denitrification in the surface-water samples showed a greater potential for loss of nitrate during the warmer months than the colder months. This pattern was similar to that seen below the streamgage at the most downstream site in the watershed. A mixed cover crop of radishes and rye was planted prior to removal (radishes) and just after harvest (rye) of the corn crop on the irrigated field. Rye grass was planted shortly after crop harvest on the dryland field. Cover crop biomass samples collected while radishes were growing and after they were killed by freezing temperatures indicates that the early planted radish crop effectively scavenged available nitrogen from the soil. Whereas radish biomass initially held more nitrogen than rye, at 55 to 8 pounds per acre, respectively, leaching of inorganic nitrate following radish die-off was minimal. Soil-water nitrate concentrations during the cover-crop growing period were lower than during the growing season prior to planting of the cover crop. There also was an increase in soil fertility and dissolved organic nitrate in samples of soil water that was likely related to increased soil microbial metabolism. Results indicate that cover crops stored plant nutrients over the winter and did not increase shallow groundwater concentrations of nitrate. Although conservation practices such as cover crops and nutrient management have been applied to these fields, there was still significant leaching of nitrate to groundwater, especially under the irrigated field. This will likely continue to be a challenge in this area and other parts of the Coastal Plain where soil moisture capacity is relatively low and managing irrigation around rainfall is difficult. Cover crops, when planted in standing corn, are one practice that can effectively pull nitrate from below the root zone to the top layer of soil, thus limiting the amount of potential nitrate leaching to groundwater. Irrigation management that would lower average soil moisture conditions during the growing season also could potentially limit nitrogen transport.

Delaware↗