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Tick-, mosquito-, and rodent-borne parasite sampling designs for the National Ecological Observatory Network

Parasites and pathogens are increasingly recognized as significant drivers of ecological and evolutionary change in natural ecosystems. Concurrently, transmission of infectious agents among human, livestock, and wildlife populations represents a growing threat to veterinary and human health. In light of these trends and the scarcity of long-term time series data on infection rates among vectors and reservoirs, the National Ecological Observatory Network (NEON) will collect measurements and samples of a suite of tick-, mosquito-, and rodent-borne parasites through a continental-scale surveillance program. Here, we describe the sampling designs for these efforts, highlighting sampling priorities, field and analytical methods, and the data as well as archived samples to be made available to the research community. Insights generated by this sampling will advance current understanding of and ability to predict changes in infection and disease dynamics in novel, interdisciplinary, and collaborative ways.

Ecosphere↗

Beyond pro-vs-anti hatchery: A typology of fishing community perceptions of salmonid hatcheries in Oregon

Objectives: Pacific salmon and steelhead hatcheries are central to fisheries management along the West Coast of North America, yet they remain a source of persistent controversy. While often framed in binary terms of pro- or anti-hatchery, public perspectives are more nuanced. Methods: We conducted 18 in-depth interviews with members of Oregon’s non-tribal fishing communities, including recreational anglers, professional fishers, non-governmental organizations, hatchery workers, and policymakers, to explore perceptions of salmonid hatcheries. Results: We developed a typology of perceptions of salmonid hatcheries in Oregon, identifying five distinct belief-based types, which we label Advocates, Reformers, Balancers, Skeptics, and Restorers. These types differed in how they evaluated hatcheries’ social and ecological impacts, governance legitimacy, and management acceptability. While Advocates and Reformers emphasized hatcheries’ social, economic, cultural, and ecological benefits, Skeptics and Restorers prioritized minimizing ecological harm and questioned hatchery effectiveness. Balancers acknowledged both social benefits and ecological risks, supporting context-specific management. All groups articulated different meanings of the term “wild,” suggesting that different perspectives across typologies may exacerbate fundamental miscommunications. Conclusion: Our findings highlight the complexity of stakeholder views and the limitations of binary framings. We suggest that tailored communication and engagement strategies, informed by typologies, may foster more inclusive and productive dialogue in hatchery policy and management.

Oregon↗

Ants as a measure of effectiveness of habitat conservation planning in southern California

In the United States multispecies habitat conservation plans were meant to be the solution to conflicts between economic development and protection of biological diversity. Although now widely applied, questions exist concerning the scientific credibility of the conservation planning process and effectiveness of the plans. We used ants to assess performance of one of the first regional conservation plans developed in the United States, the Orange County Central‐Coastal Natural Community Conservation Plan (NCCP), in meeting its broader conservation objectives of biodiversity and ecosystem‐level protection. We collected pitfall data on ants for over 3 years on 172 sites established across a network of conservation lands in coastal southern California. Although recovered native ant diversity for the study area was high, site‐occupancy models indicated the invasive and ecologically disruptive Argentine ant ( Linepithema humile ) was present at 29% of sites, and sites located within 200 m of urban and agricultural areas were more likely to have been invaded. Within invaded sites, native ants were largely displaced, and their median species richness declined by more than 60% compared with uninvaded sites. At the time of planning, 24% of the 15,133‐ha reserve system established by Orange County NCCP fell within 200 m of an urban or agricultural edge. With complete build out of lands surrounding the reserve, the proportion of the reserve system vulnerable to invasion will grow to 44%. Our data indicate that simply protecting designated areas from development is not enough. If habitat conservation plans are to fulfill their conservation promise of ecosystem‐level protection, a more‐integrated and systematic approach to the process of habitat conservation planning is needed .

California↗

Biodiversity in a changing climate: a synthesis of current and projected trends in the US

This paper provides a synthesis of the recent literature describing how global biodiversity is being affected by climate change and is projected to respond in the future. Current studies reinforce earlier findings of major climate-change-related impacts on biological systems and document new, more subtle after-effects. For example, many species are shifting their distributions and phenologies at faster rates than were recorded just a few years ago; however, responses are not uniform across species. Shifts have been idiosyncratic and in some cases counterintuitive, promoting new community compositions and altering biotic interactions. Although genetic diversity enhances species' potential to respond to variable conditions, climate change may outpace intrinsic adaptive capacities and increase the relative vulnerabilities of many organisms. Developing effective adaptation strategies for biodiversity conservation will not only require flexible decision-making and management approaches that account for uncertainties in climate projections and ecological responses but will also necessitate coordinated monitoring efforts.

Frontiers in Ecology and the Environment↗

Disease emergence and resurgence—the wildlife-human connection

In 2000, the Global Outbreak Alert and Response Network (GOARN) was organized as a global disease watchdog group to coordinate disease outbreak information and health crisis response. The World Health Organization (WHO) is the headquarters for this network. Understandably, the primary focus for WHO is human health. However, diseases such as the H5N1 avian influenza epizootic in Asian bird populations demonstrate the need for integrating knowledge about disease emergence in animals and in humans. Aside from human disease concerns, H5N1 avian influenza has major economic consequences for the poultry industry worldwide. Many other emerging diseases, such as severe acute respiratory syndrome (SARS), monkeypox, Ebola fever, and West Nile fever, also have an important wildlife component. Despite these wildlife associations, the true integration of the wildlife component in approaches towards disease emergence remains elusive. This separation between wildlife and other species’ interests is counterproductive because the emergence of zoonotic viruses and other pathogens maintained by wildlife reservoir hosts is poorly understood. This book is about the wildlife component of emerging diseases. It is intended to enhance the reader’s awareness of the role of wildlife in disease emergence. By doing so, perhaps a more holistic approach to disease prevention and control will emerge for the benefit of human, domestic animal, and free-ranging wildlife populations alike. The perspectives offered are influenced by more than four decades of my experiences as a wildlife disease practitioner. Although wildlife are victims to many of the same disease agents affecting humans and domestic animals, many aspects of disease in free-ranging wildlife require different approaches than those commonly applied to address disease in humans or domestic animals. Nevertheless, the broader community of disease investigators and health care professionals has largely pursued a separatist approach for human, domestic animal, and wildlife rather than embracing the periodically proposed concept of “one medicine.” We especially need to embrace this concept as the human population increases because there will be more contact, direct and indirect, among humans, domestic animals, and wildlife. An “Ecology for a Crowded Planet” will be an even more pressing concern, and that includes increasing our understanding of disease ecology, especially that of the zoonoses.

Circular↗

Seasonal dynamics of zooplankton in Columbia–Snake River reservoirs,with special emphasis on the invasive copepod Pseudodiaptomus forbesi

The Asian copepod Pseudodiaptomus forbesi has recently become established in the Columbia River. However, little is known about its ecology and effects on invaded ecosystems. We undertook a 2-year (July 2009 to June 2011) field study of the mesozooplankton in four reservoirs in the Columbia and Snake Rivers, with emphasis on the relation of the seasonal variation in distribution and abundance of P. forbesi to environmental variables. Pseudodiaptomus forbesi was abundant in three reservoirs; the zooplankton community of the fourth reservoir contained no known non-indigenous taxa. The composition and seasonal succession of zooplankton were similar in the three invaded reservoirs: a bloom of rotifers occurred in spring, native cyclopoid and cladoceran species peaked in abundance in summer, and P. forbesi was most abundant in late summer and autumn. In the uninvaded reservoir, total zooplankton abundance was very low year-round. Multivariate ordination indicated that temperature and dissolved oxygen were strongly associated with zooplankton community structure, with P. forbesi appearing to exhibit a single generation per year . The broad distribution and high abundance of P. forbesi in the Columbia–Snake River System could result in ecosystem level effects in areas intensively managed to improve conditions for salmon and other commercially and culturally important fish species.

Columbia River, Snake River↗

Developing an effective Agassiz's Desert Tortoise monitoring program: Final report to the Coachella Valley Conservation Commission

Agassiz’s desert tortoise (Gopherus agassizii) is a conservation-reliant species with populations north and west of the Colorado River protected as threatened under the Endangered Species Act (Averill-Murray et al. 2012). Since it was listed under this category in 1990, a great deal has been learned about the natural history of the species, and it is now one of the best-studied turtles in the United States (Lovich and Ennen 2013). However, the accumulated body of scientific data available for the species has not yet been translated into recovery or delisting of the species. Successful conservation of any species requires knowledge of their natural history and how vital rates affect their ability to maintain stable populations in the face of natural and anthropogenic stresses. Agassiz’s desert tortoises occur from southwestern Utah to near the Mexican border in California – a distance of over 450 km – but population densities vary greatly across this immense landscape (U.S. Fish and Wildlife Service 2015). Tortoises occur in the Sonoran Desert of California, including the eastern and western ends of the Coachella Valley, where it is one of 27 species covered under the Coachella Valley Multiple Species Habitat Conservation Plan and Natural Community Conservation Plan (CVMSHCP/NCCP). The southern portion of Joshua Tree National Park (JTNP) lies within this 1.1 million acre planning area, and was predicted to be an area of low-density tortoise populations using habitat suitability modeling (Barrows 2011). JTNP is near the southern distributional limit of G. agassizii, yet very little has been published regarding the ecology of tortoises in the Sonoran Desert of California. Reproductive output is an important gross measure of the ability of a population to persist. When integrated with data on fertility and survivorship, this information forms a foundation for assessing population status and formulating effective management strategies (e.g., Congdon et al. 1993, 1994), especially for imperiled species. One aspect of the biology of G. agassizii that has been particularly well-studied is reproductive output. However, most of what we know about this topic comes from research in the Mojave Desert portion of the species’ range (Ernst and Lovich 2009). Comparatively little has been published on the reproductive ecology of populations living in the Sonoran Desert ecosystem of California. Publications by Lovich et al. (1999, 2011, 2012, 2014, 2015) constitute the main body of literature on desert tortoise reproductive ecology in the Sonoran Desert of California, with one study population located at the western end of the CVMSHCP/NCCP area. Collecting data on Agassiz’s desert tortoise ecology in the Sonoran Desert ecosystem is important due to significant differences between the two adjacent desert ecosystems, especially the timing and amounts of annual precipitation, and their potential effects on reproductive output (e.g., Lovich et al. 5 2015). There are also differences in the vulnerability of tortoises to the effects of a warming, drying climate between the two deserts (Barrows 2011; Zylstra et al. 2012). The overall goal of this study was to collect data on demography, reproductive output, and genetic affinities at a study site in the Sonoran Desert portion of JTNP in the eastern end of the CVMSHCP/NCCP area. Specific objectives included: 1) Collect data to establish baselines on tortoise populations and/or their habitat suitability in core habitat within the CVNCCP area, including biotic and abiotic variables affecting persistence of tortoise populations; 2) Compare and contrast with data collected on desert tortoises at USGS/BLM study site near Palm Springs over 16 years; 3) Support long-term modeling efforts needed to determine tortoise population viability; 4) Refine modeled relationships with identified threats such as fire, invasive species and climate change; and 5) Prioritize adaptive management needs for the desert tortoise in and beyond the CVNCCP area. The data from this study will aid in determining baseline estimates of the desert tortoise population size within the planning area as well as establish a marked population of Agassiz’s desert tortoises for future monitoring. Data will be integrated with habitat modeling in order to refine model output. Genetic data will be collected on both the north and south sides of Interstate 10 to determine the potential effects of habitat fragmentation and genetic mixing. Analyses are ongoing and results beyond those presented in this report will be published in peer-reviewed scientific journals following inclusion of additional data collected on the south side of Shavers Valley in 2017-2018.

Report↗

A promising tool for subsurface permafrost mapping-An application of airborne geophysics from the Yukon River Basin, Alaska

Permafrost is a predominant physical feature of the Earth's Arctic and Subarctic clines and a major consideration encompassing ecosystem structure to infrastructure engineering and placement. Perennially frozen ground is estimated to cover about 85 percent of the state of Alaska where northern reaches are underlain with continuous permafrost and parts of interior Alaska are underlain by areas of discontinuous and (or) sporadic permafrost (fig. 1). The region of Interior Alaska, where permafrost is scattered among unfrozen ground, is a complex mosaic of terrains and habitats. Such diversity creates arrays of lakes and surface-water and groundwater patterns that continental populations of migratory waterfowl and internationally significant fisheries have adapted to over time. A road or pipeline might pass over frozen and unfrozen ground, affecting the types of materials and engineering approaches needed to sustain the infrastructure. Effective mapping of discontinuous permafrost at scales meaningful ecologically and (or) from an engineering perspective has been a long-standing challenge. Using techniques such as borehole logging for site-specific assessments or botanical techniques that can suggest underlying permafrost distributions can be labor intensive and difficult to accomplish at the scale and remoteness of much of Alaska. The climate is changing in the Arctic and Subarctic regions. The warming observed throughout much of Alaska could create widespread changes in permafrost. How the warming of the permafrost affects near-surface processes, ecosystems, and community infrastructure and ecosystems is not clear. A better understanding of the dynamic distribution and physical properties of permafrost, from continuous to discontinuous, will provide knowledge of how the permafrost environment may change in the future and help inform engineering and natural resource response strategies. Here we discuss an application of an airborne remote sensing methodology for mapping and shade imaging permafrost characteristics at various scales. This work provides the first look into three-dimensional distribution of permafrost in the areas around Fort Yukon and is a demonstration of the application of airborne electromagnetic (AEM) to permafrost mapping. Such an approach is attractive, particularly in Arctic and Subarctic studies, where ground access is difficult and ecosystems are fragile. In June 2010, the U.S. Geological Survey (USGS) conducted an AEM survey near Fort Yukon, Alaska. The primary focus of this survey was to map the distribution of permafrost in selected areas in order to supply information for the development of groundwater models of the Yukon River Basin. However, the methodologies have more far-reaching ecological and engineering applications. Approximately 1,800 line kilometers were acquired in a combination of typical block style surveying in the immediate area of Fort Yukon and in long reconnaissance lines over a broader area. The widely spaced lines were flown to cross the modern Yukon River in "X" like patterns with intersections at features that have been previously studied (fig. 2). AEM is used to gather data on the electrical resistivity of materials in the subsurface below the flight path of the helicopter, which are then analyzed to interpret the subsurface lithology and the location and extent of permafrost. For this survey, the electrical resistivity was imaged to depths on the order of 50-100 meters. Images from the survey can be qualitatively compared with known permafrost features and suggest new permafrost features. Electrical properties of earth materials are affected by lithology as well as temperature and the presence of ice; frozen materials become substantially more resistive. This allows for the identification of permafrost from the resistivity image (Abraham and others, 2011). In the area of Fort Yukon, the AEM survey shows elevated resistivities extending to depth, likely indicative of thick permafrost. This depth corresponds well to observations from a borehole drilled in the area in the late 1990s, which detected permafrost to a depth of about 100 meters (Clark and others, 2009). In contrast to the area of Fort Yukon, the Yukon River and its floodplain are not associated with deep resistive sediments, suggesting a lack of deep permafrost, at least within the depth range of the AEM mapping (fig. 3).

Yukon River Basin↗

CrustCheck: A conceptual framework for interpreting biocrust trait–function relationships in drylands

Introduction Biocrusts are increasingly recognized for their contribution to soil stability, hydrology, nutrient cycling, and energy balance in drylands, yet practical field methods for assessing functional roles are limited. Existing approaches typically rely on cover estimates, taxonomic identification, or laboratory analyses, providing little insight into functional variation among biocrust types. To address this gap, we developed CrustCheck, a literature-derived conceptual framework that synthesizes potential relationships between easily observable biocrust traits and key ecological functions. The framework provides a foundation for future empirical validation of these relationships. Objectives We aimed to: (1) identify visual characteristics of biocrusts potentially associated with key ecological functions, and (2) synthesize these relationships into a literature-derived conceptual framework for comparative interpretation of potential biocrust contributions to landscape function. Methods We conducted a structured review of global literature on biocrusts' contribution to landscape function, drawing on multiple databases, targeted journals, and identifying relevant scientific publications from reference lists. We screened sources for information on relationships between visual traits and metrics of landscape function, synthesized relationships thematically, and used them to develop a multi-trait, function-oriented conceptual framework to inform further testing of these hypotheses in the field. Results Our review revealed that visual traits such as thickness/height, patchiness, cover, and surface roughness emerged as potential indicators of surface stability, hydrological function, and nutrient accumulation. Community composition was also frequently associated with these functions, while color reflected energy balance and carbon fixation potential. Conclusions Literature reveals that visual traits of biocrusts may serve as proxies for landscape function. CrustCheck is presented here as a conceptual synthesis framework intended to support interpretation of potential trait–function relationships rather than as a quantitatively validated predictive tool.

Restoration Ecology↗

Fire and amphibians in North America

Information on amphibian responses to fire and fuel reduction practices is critically needed due to potential declines of species and the prevalence of new, more intensive fire management practices in North American forests. The goals of this review are to summarize the known and potential effects of fire and fuels management on amphibians and their aquatic habitats, and to identify information gaps to help direct future scientific research. Amphibians as a group are taxonomically and ecologically diverse; in turn, responses to fire and associated habitat alteration are expected to vary widely among species and among geographic regions. Available data suggest that amphibian responses to fire are spatially and temporally variable and incompletely understood. Much of the limited research has addressed short-term (1–3 years) effects of prescribed fire on terrestrial life stages of amphibians in the southeastern United States. Information on the long-term negative effects of fire on amphibians and the importance of fire for maintaining amphibian communities is sparse for the majority of taxa in North America. Given the size and severity of recent wildland fires and the national effort to reduce fuels on federal lands, future studies are needed to examine the effects of these landscape disturbances on amphibians. We encourage studies to address population-level responses of amphibians to fire by examining how different life stages are affected by changes in aquatic, riparian, and upland habitats. Research designs need to be credible and provide information that is relevant for fire managers and those responsible for assessing the potential effects of various fuel reduction alternatives on rare, sensitive, and endangered amphibian species.

Forest Ecology and Management↗

Lichens and microfungi in biocrusts: Structure and function now and in the future

Biological soil crusts (biocrusts) are formed by soil-surface communities of biota that live within, or immediately on top of, the uppermost millimeters of soil. They consist of cyanobacteria, algae, mosses, microfungi, and lichenized fungi (hereafter, lichens). Cyanobacterial and microfungal filaments, rhizinae and rhizomorphs of lichens, and rhizinae and protonemata of bryophytes weave throughout the top few millimeters of soil, gluing loose soil particles together (Fig. 1). The intimate association between soil particles and organisms forms a coherent crust. A quantitative estimate of global biological crust cover is difficult to obtain and not yet available, but the worldwide coverage of the terrestrial surface by biocrusts is very high. In arid and semiarid areas, biocrusts may constitute up to or more than 70% of the living cover and dryland (hyper-arid, arid, semi-arid, and polar deserts) ecosystems, where they often dominate, cover ~40% of the terrestrial land mass (Pointing and Belnap 2014). Lichens and microfungi are an essential and often dominant part of biocrusts. About one fifth (19 %) of all known species of fungi are lichenized; that is, they form a stable symbiotic association with green algal or/and cyanobacterial photobionts that provide nutrients for the mycobiont (fungi). The vast majority of lichenized fungi belong to the Ascomycota, with 42% of all fungi in this group forming lichens (Kirk et al., 2001). About 85% of lichen-forming fungi are symbiotic with Chlorophyta (green algae, creating "chlorolichens), approximately 10% with Cyanophyta, (creating "cyanolichens"), and the remainder are associated simultaneously with both groups. About 40 genera of photobionts have been identified in lichens: 25 are green algae and 15 are cyanobacteria. The autotrophic lifestyle of lichens requires an exposure of the green thallus to light. Most lichens are long-lived organisms with high habitat specificity. They are especially ecologically successful in dryland areas where competition with phanerogamous vegetation is reduced. It is estimated that approximately 8% of the earth's terrestrial surface has lichens as its most dominant life-form (Ahmadjian 1995). One of their most important habitats are biocrusts, which lichens often dominate. In the present Chapter we concentrate on those widely distributed biocrusts in which free-living and lichenized fungi play a dominating role. We describe their community structure, analyze the special properties and functions of these organisms as key members of biocrusts, and then discuss the function of the fungi-rich biocrusts as components of larger ecosystems and landscapes (for details and specific literature, see Belnap and Lange 2003).

Book chapter↗

Leveraging community science data for population assessments during a pandemic

The COVID-19 pandemic has disrupted field research programs, making conservation and management decision-making more challenging. However, it may be possible to conduct population assessments using integrated models that combine community science data with existing data from structured surveys. We developed a space-time integrated model to characterize spatial and temporal variability in population distribution. We fit our integrated model to 10 years of eBird (2010-2020) and 9 years of aerial survey (2010-2019) mottled duck count data to forecast 2020 population size along the western Gulf Coast of Texas and Louisiana. Estimates of mottled duck abundance were similar in magnitude to estimates calculated using previous methods, but were more precise and showed evidence of a declining population. The spatial distribution for mottled ducks each year was characterized by several concentrations of relatively high abundance, although the location of these abundance ‘hotspots’ varied over time. Expected abundance was higher for areas with a higher proportion of area covered by marsh habitat. By leveraging large-scale community science data, we were able to conduct a population assessment despite the disruption in structured surveys caused by the pandemic. As participation in community science platforms continues to increase, we anticipate modeling frameworks, like the integrated model we developed here, will become increasingly useful for informing conservation and management decision-making.

Louisiana, Texas↗

Leaf fluctuating asymmetry, soil disturbance and plant stress: A multiple year comparison using two herbs, Ipomoea pandurata and Cnidoscolus stimulosus

We studied Cnidoscolus stimulosus and Ipomoea pandurata, two common herbs of the Fall Line Sandhills to assess their potential as ecosystem level stress indicators. We focused on plants because they are among the most persistent organisms in terrestrial ecosystems. We used developmental instability as an indicator of plant population stress. Developmental instability is usually measured as deviations from symmetry, in traits that normally develop symmetrically. Thus, symmetry represents an idealized a priori phenotype. Stress presumably causes perturbations during development that may exceed the capacity of the organism to buffer or correct, resulting in developmental instability, and hence deviations from this ideal. Soil disturbance imposed by different land use patterns at Fort Benning, Georgia provided a gradient of soil disturbance. In 2000-2002 we collected plants from nine different sites representing three levels of disturbance. In addition, in 2002 we collected microhabitat data in 1 m quadrats surrounding each plant whose developmental stability we also assessed. The developmental instability of both species was influenced by land use patterns, whether or not the sites had been previously burned, and microhabitat variables. Developmental instability increased with soil disturbance, burning in the prior year, and as the percentage of bare ground increased around the target individual. To some extent, favorable microhabitat conditions reduced developmental instability in sites with medium and high soil disturbance, whereas unfavorable conditions at low soil disturbance sites increased developmental instability. As an indicator of community level stress, developmental instability is best used in conjunction with other indices of environmental quality. ?? Published by Elsevier Ltd.

Ecological Indicators↗

Species richness, equitability, and abundance of ants in disturbed landscapes

Ants are used as indicators of environmental change in disturbed landscapes, often without adequate understanding of their response to disturbance. Ant communities in the southeastern United States displayed a hump-backed species richness curve against an index of landscape disturbance. Forty sites at Fort Benning, in west-central Georgia, covered a spectrum of habitat disturbance (military training and fire) in upland forest. Sites disturbed by military training had fewer trees, less canopy cover, more bare ground, and warmer, more compact soils with shallower A-horizons. We sampled ground-dwelling ants with pitfall traps, and measured 15 habitat variables related to vegetation and soil. Ant species richness was greatest with a relative disturbance of 43%, but equitability was greatest with no disturbance. Ant abundance was greatest with a relative disturbance of 85%. High species richness at intermediate disturbance was associated with greater within-site spatial heterogeneity. Species richness was also associated with intermediate values of the normalized difference vegetation index (NDVI), a correlate of net primary productivity (NPP). Available NPP (the product of NDVI and the fraction of days that soil temperature exceeded 25 ??C), however, was positively correlated with species richness, though not with ant abundance. Species richness was unrelated to soil texture, total ground cover, and fire frequency. Ant species richness and equitability are potential state indicators of the soil arthropod community. Moreover, equitability can be used to monitor ecosystem change. ?? 2008 Elsevier Ltd.

Ecological Indicators↗

Relations between benthic community structure and metals concentrations in aquatic macroinvertebrates: Clark Fork River, Montana

We sampled macroinvertebrate communities at six sites on the upper Clark Fork River, Montana, to determine relations between macroinvertebrate community structure and metals in invertebrates and the best benthic community metrics to use for ranking sites based on the relative severity of the effects of metals. Concentrations (μg/g) of six metals in invertebrates were determined: Al (range = 591–4193), As (2.7–34.1), Cd (0.13–8.38), Cu (26–1382), Pb (0.54–67.1), and Zn (212–1665). Concentrations of As, Cd, Cu, Pb, and total metals were significantly correlated with at least one benthic metric. Copper (r = 0.88–0.94) and total metals (r = 0.90–0.97) provided the most highly significant correlations. Based on longitudinal site comparisons of metals in invertebrates, benthic community structure, and differences between proportionally scaled ranks, five benthic metrics provided the best indicators of relative impact: taxa richness, Ephemeroptera-Plecoptera-Trichoptera (EPT) richness, chironomid richness, percentage of the most dominant taxon, and density. The two sites with the highest accumulations of invertebrate metals also demonstrated the greatest relative degree of impact based on these parameters. The most meaningful combinations of metrics indicate that the benthic community at the most upstream site is being severely impacted by metals. Two sites demonstrated little or no negative impact, and three sites demonstrated low or moderate levels of negative impacts, which may be due to a combination of metals and other factors such as organic enrichment. We recommend that benthic community structure and metals in invertebrates collected from riffle habitats be used to determine relative impacts in metals-contaminated river systems, owing to their close relation to metal availability and transfer to higher trophic levels.

Montana↗

Assessing simulated ecosystem processes for climate variability research at Glacier National Park, USA

Glacier National Park served as a test site for ecosystem analyses that involved a suite of integrated models embedded within a geographic information system. The goal of the exercise was to provide managers with maps that could illustrate probable shifts in vegetation, net primary production (NPP), and hydrologic responses associated with two selected climatic scenarios. The climatic scenarios were (a) a recent 12-yr record of weather data, and (b) a reconstituted set that sequentially introduced in repeated 3-yr intervals wetter–cooler, drier–warmer, and typical conditions. To extrapolate the implications of changes in ecosystem processes and resulting growth and distribution of vegetation and snowpack, the model incorporated geographic data. With underlying digital elevation maps, soil depth and texture, extrapolated climate, and current information on vegetation types and satellite-derived estimates of leaf area indices, simulations were extended to envision how the park might look after 120 yr. The predictions of change included underlying processes affecting the availability of water and nitrogen. Considerable field data were acquired to compare with model predictions under current climatic conditions. In general, the integrated landscape models of ecosystem processes had good agreement with measured NPP, snowpack, and streamflow, but the exercise revealed the difficulty and necessity of averaging point measurements across landscapes to achieve comparable results with modeled values. Under the extremely variable climate scenario significant changes in vegetation composition and growth as well as hydrologic responses were predicted across the park. In particular, a general rise in both the upper and lower limits of treeline was predicted. These shifts would probably occur along with a variety of disturbances (fire, insect, and disease outbreaks) as predictions of physiological stress (water, nutrients, light) altered competitive relations and hydrologic responses. The use of integrated landscape models applied in this exercise should provide managers with insights into the underlying processes important in maintaining community structure, and at the same time, locate where changes on the landscape are most likely to occur.

Montana↗

Saltwater intrusion and sea level rise threatens U.S. rural coastal landscapes and communities

The United States (U.S.) coastal plain is subject to rising sea levels, land subsidence, more severe coastal storms, and more intense droughts. These changes lead to inputs of marine salts into freshwater-dependent coastal systems, creating saltwater intrusion. The penetration of salinity into the coastal interior is exacerbated by groundwater extraction and the high density of agricultural canals and ditches throughout much of the rural U.S. landscape. Together saltwater intrusion and sea level rise (SWISLR) create substantial changes to the social-ecological systems situated along the coastal plain. Many scholars and practitioners are engaged in studying and managing SWISLR impacts on social, economic, and ecological systems. However, most efforts are localized and disconnected, despite a widespread desire to understand this common threat. In addition to variable rates of sea level rise across the U.S. outer coastal plain, differences in geomorphic setting, water resources infrastructure and management, and climate extremes are resulting in different patterns of saltwater intrusion. Understanding both the absolute magnitude of this rapid environmental change, and the causes and consequences for its spatial and temporal variation presents an opportunity to build new mechanistic models to link directional climate change to temporally and spatially dynamic socio-environmental impacts. The diverse trajectories of change offer rich opportunities to test and refine modern theories of ecosystem state change in systems with exceptionally strong socioecological feedbacks.

Anthropocene↗

Feeding ecology of pelagic larval Burbot in Northern Lake Huron, Michigan

Burbot Lota lota are a key demersal piscivore across the Laurentian Great Lakes whose populations have declined by about 90% in recent decades. Larval Burbot typically hatch in the early spring and rely on abundant crustacean zooplankton prey. We examined the stomach contents of larval Burbot from inshore (≤15 m) and offshore sites (37 and 91 m) in northern Lake Huron, Michigan. Concurrent zooplankton vertical tows at the same sites showed that the prey community was dominated by calanoid copepods, dreissenid mussel veligers, and rotifers. Burbot consumed mostly cyclopoid copepods, followed by copepod nauplii and calanoid copepods. Chesson's index of selectivity was calculated and compared among sites and months for individual Burbot. According to this index, larval Burbot exhibited positive selection for cyclopoid copepods and copepod nauplii and negative selection for calanoid copepods, cladocerans, rotifers, and dreissenid veligers. This selectivity was consistent across sites and throughout the sampling period. Burbot displayed little variation in their prey preferences during the larval stage, which suggests that the recent shifts in zooplankton abundance due to the invasion of the predatory zooplankter Bythotrephes longimanus and competition from invasive Rainbow Smelt Osmerus mordax could negatively impact larval Burbot populations.

Michigan↗