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At least 1,405 records · Page 78Linked to original sources
USGS1987

Pressure sensitivity of low permeability sandstones

Detailed core analysis has been carried out on 32 tight sandstones with permeabilities ranging over four orders of magnitude (0.0002 to 4.8 mD at 5000 psi confining pressure). Relationships between gas permeability and net confining pressure were measured for cycles of loading and unloading. For some samples, permeabilities were measured both along and across bedding planes. Large variations in stress sensitivity of permeability were observed from one sample to another. The ratio of permeability at a nominal confining pressure of 500 psi to that at 5000 psi was used to define a stress sensitivity ratio. For a given sample, confining pressure vs permeability followed a linear log-log relationship, the slope of which provided an index of pressure sensitivity. This index, as obtained for first unloading data, was used in testing relationships between stress sensitivity and other measured rock properties. Pressure sensitivity tended to increase with increase in carbonate content and depth, and with decrease in porosity, permeability and sodium feldspar. However, scatter in these relationships increased as permeability decreased. Tests for correlations between pressure sensitivity and various linear combinations of variables are reported. Details of pore structure related to diagenetic changes appears to be of much greater significance to pressure sensitivity than mineral composition.

Journal of Petroleum Science and Engineering↗
USGS2025

Performance analysis of oil recovery and CO2 retention in a greenfield residual oil zone: CO2-EOR in Tall Cotton Field (Permian Basin, West Texas, USA)

Residual oil zones (ROZs) can offer significant oil resources via enhanced oil recovery (EOR) as well as subsurface carbon dioxide (CO 2 ) retention during injection. If injected CO 2 is anthropogenic, the ROZs can offer a substantial geologic storage potential. The ROZs below the oil/water contact (OWC) of main pay zones (MPZ) in conventional reservoirs or brownfields, are more commonly developed for CO 2 injection and oil production and reported in the literature. However, CO 2 -EOR in greenfield ROZs, reservoirs without a MPZ present, have rarely been developed for CO 2 -EOR operation. The Tall Cotton Field of West Texas, Permian Basin, which started production in 2015 (Phase 1) and expanded in 2017 (Phase 2) from the San Andres Limestone, is one of the first examples of greenfield ROZs developed for EOR by injecting CO 2 . This paper analyses EOR and CO 2 retention performance of Tall Cotton Field using allocated injection and production data from inverted 5-spot well patterns of Phase-1 and -2 developments. Production and injection data allocated to each of the 28 identified patterns (nine 20-acre patterns for Phase-1, three 20-acre and sixteen 10-acre patterns for Phase-2) were analyzed for historical and forecasted oil recovery using ratio-trend decline analysis, and for CO 2 retention performance of the patterns. The allocated data were further used to calculate injected reservoir pore volume and void replacement ratios (VRR) for the analysis period. Quantitative results indicated that oil recovery factors of the 5-spot patterns varied between 4–10 %, and 5–30 % between the end of injection and the forecast periods, respectively. Storage of CO 2 , on the other hand, increased to a mean value of ∼7130 MMscf per pattern in Phase-1 and to a mean storage of 3700 MMscf per pattern in Phase-2 until the end of injection, followed by a decline after the end of injection and into the forecast period. Resulting CO 2 utilization factors ∼6–50 Mscf/bbl were estimated at the end of injection. Overall, presented results suggested that developing greenfield ROZs for CO 2 -EOR can be as promising as brownfield ROZs and mature MPZs for EOR and underground storage of injected CO 2 . For Tall Cotton Field, results suggest that Phase-2 patterns generally outperformed Phase-1 for oil recovery factors, while Phase-1 performed better in CO 2 retention performance metrics. This is the first study in the literature that reports a detailed CO 2 -EOR performance analysis of a greenfield ROZ in the Permian Basin, which can potentially allow for comparison with MPZs and brownfield ROZs.

Texas↗
USGS2019

Constraining the oxygen isotopic composition of nitrate produced by nitrification

Measurements of the stable isotope ratios of nitrogen ( 15 N/ 14 N) and oxygen ( 18 O/ 16 O) in nitrate (NO 3 – ) enable identification of sources, dispersal, and fate of natural and contaminant NO 3 – in aquatic environments. The 18 O/ 16 O of NO 3 – produced by nitrification is often assumed to reflect the proportional contribution of oxygen atom sources, water, and molecular oxygen, in a 2:1 ratio. Culture and seawater incubations, however, indicate oxygen isotopic equilibration between nitrite (NO 2 – ) and water, and kinetic isotope effects for oxygen atom incorporation, which modulate the NO 3 – 18 O/ 16 O produced during nitrification. To investigate the influence of kinetic and equilibrium effects on the isotopic composition of NO 3 – produced from the nitrification of ammonia (NH 3 ), we incubated streamwater supplemented with ammonium (NH 4 + ) and increments of 18 O-enriched water. Resulting NO 3 – 18 O/ 16 O ratios showed (1) a disproportionate sensitivity to the 18 O/ 16 O ratio of water, mediated by isotopic equilibration between water and NO 2 – , as well as (2) kinetic isotope discrimination during O atom incorporation from molecular oxygen and water. Empirically, the NO 3 – 18 O/ 16 O ratios thus produced fortuitously converge near the 18 O/ 16 O ratio of water. More elevated NO 3 – 18 O/ 16 O values commonly reported in soils and oxic groundwater may thus derive from processes additional to nitrification, including NO 3 – reduction.

Environmental Science & Technology↗
USGS2006

Anaerobic biotransformation of roxarsone and related N-substituted phenylarsonic acids

Large quantities of arsenic are introduced into the environment through land application of poultry litter containing the organoarsenical feed additive roxarsone (3-nitro-4-hydroxyphenylarsonic acid). The objective of this study was to evaluate the bioconversion of roxarsone and related N-substituted phenylarsonic acid derivatives under anaerobic conditions. The results demonstrate that roxarsone is rapidly transformed in the absence of oxygen to the corresponding aromatic amine, 4-hydroxy-3-aminophenylarsonic acid (HAPA). The formation of HAPA is attributable to the facile reduction of the nitro group. Electron-donating substrates, such as hydrogen gas, glucose, and lactate, stimulated the rate of nitro group reduction, indicating a microbial role. During long-term incubations, HAPA and the closely related 4-aminophenylarsonic acid (4-APA) were slowly biologically eliminated by up to 99% under methanogenic and sulfate-reducing conditions, whereas little or no removal occurred in heat-killed inoculum controls. Arsenite and, to a lesser extent, arsenate were observed as products of the degradation. Freely soluble forms of the inorganic arsenical species accounted for 19-28% of the amino-substituted phenylarsonic acids removed. This constitutes the first report of a biologically catalyzed rupture of the phenylarsonic group under anaerobic conditions. ?? 2006 American Chemical Society.

Environmental Science & Technology↗
USGS2005

Scientists in action

News about careers in the natural sciences -- from mapping the planets to sampling the ocean floor, from protecting wildlife to forecasting volcanic eruptions.

General Information Product↗
USGS2000

U.S. Geological Survey Library classification system

The U.S. Geological Survey Library classification system has been designed for earth science libraries. It is a tool for assigning call numbers to earth science and allied pure science materials in order to collect these materials into related subject groups on the library shelves and arrange them alphabetically by author and title. The classification can be used as a retrieval system to access materials through the subject and geographic numbers. The classification scheme has been developed over the years since 1904 to meet the ever-changing needs of increased specialization and the development of new areas of research in the earth sciences. The system contains seven schedules: Subject schedule Geological survey schedule Earth science periodical schedule Government document periodical schedule General science periodical schedule Earth science map schedule Geographic schedule Introduction provides detailed instructions on the construction of call numbers for works falling into the framework of the classification schedules. The tables following the introduction can be quickly accessed through the use of the newly expanded subject index. The purpose of this publication is to provide the earth science community with a classification and retrieval system for earth science materials, to offer sufficient explanation of its structure and use, and to enable library staff and clientele to classify or access research materials in a library collection.

Bulletin↗
USGS1992

U.S. Geological Survey Library classification system

The U.S. Geological Survey library classification system has been designed for an earth science library. It is a tool for assigning classification numbers to earth science and allied pure science library materials in order to collect these materials into related subject groups on the library shelves and arrange them alphabetically by author and title. It can also be used as a retrieval system to access these materials through the subject and visible geographic classification numbers. The classification scheme has been developed over the years since 1904 to meet the ever-changing needs of increased specialization and new areas of study in the earth sciences. This system contains seven schedules: Subject schedule Geological survey schedule Earth science periodical schedule Government documents periodical schedule General science periodical schedule Earth science maps schedule Geographic schedule A geographic number, from the geographic schedule, is distinguished from other numbers in the system in that it is always enclosed in parentheses; for example, (200) is the geographic number for the United States. The geographic number is used in conjunction with the six other previously listed schedules, and it represents slightly different nuances of meanings, in respect to geographic locale, for each schedule. When used with a subject number, the geographic number indicates the country, state, province, or region in which the research was made. The subject number, 203, geology, when combined with the geographic number, (200), for example 203(200), is the classification number for library materials on the geology of the United States. The geographic number, combined with the capital letter G, for example, G(211), is the classification number for an earth science periodical issued by a geological association or university geology department in the State of Maine. When the letter S is combined with a geographic number, for example, S(276), it represents a general science periodical for a university or association in California. When the letter P is combined with a geographic number, for example, P(200), it represents a governmental periodical issued by the United States Federal Government. Geographic numbers standing alone represent classification numbers for the publications of geological surveys; for example, (200) represents publications of the U.S. Geological Survey. Map call numbers have a geographic number preceded by the capital letter M, followed by an abbreviated subject number. For example: M(200)2 where: M = Map (200) = Geographic region of the United States 2 = Abbreviation for the subject number 203— geology. The introduction, which follows this abstract, provides detailed procedures on the construction of complete call numbers for works falling into the framework of the aforesaid classification schedules. The tables following the introduction can be quickly accessed through the use of the newly expanded subject index. The purpose of this publication is to provide the earth science community with a classification and retrieval system for earth science materials, to provide sufficient explanation of its structure and use, and to enable library staff and clientele to classify or access research materials in a library collection.

Bulletin↗
USGS2009

Recharge rates and chemistry beneath playas of the High Plains aquifer: A literature review and synthesis

Playas are ephemeral, closed-basin wetlands that are important zones of recharge to the High Plains (or Ogallala) aquifer and critical habitat for birds and other wildlife in the otherwise semiarid, shortgrass prairie and agricultural landscape. The ephemeral nature of playas, low regional recharge rates, and a strong reliance on ground water from the High Plains aquifer has prompted many questions regarding the contribution of recharge from playas to the regional aquifer. To address these questions and concerns, the U.S. Geological Survey, in cooperation with the Playa Lakes Joint Venture, present a review and synthesis of the more than 175 publications about recharge rates and chemistry beneath playas and interplaya settings. Although a number of questions remain regarding the controls on recharge rates and chemistry beneath playas, the results from most published studies indicate that recharge rates beneath playas are substantially (1 to 2 orders of magnitude) higher than recharge rates beneath interplaya settings. The synthesis presented here supports the conceptual model that playas are important zones of recharge to the High Plains aquifer and are not strictly evaporative pans. The major findings of this synthesis yield science-based implications for the protection and management of playas and ground-water resources of the High Plains aquifer and directions for future research.

High Plains aquifer↗
USGS2025

Range-wide population trend analysis for greater sage-grouse (Centrocercus urophasianus)—Updated 1960–2024

Greater sage-grouse ( Centrocercus urophasianus ; hereafter sage-grouse) are at the center of State and national land-use policies largely because of their unique life-history traits as an ecological indicator for the health of sagebrush ecosystems. This updated population trend analysis provides State and Federal land and wildlife managers with the best available science to help guide management and conservation plans aimed at benefiting sage-grouse populations and the ecosystems they inhabit. This analysis relied on previously published population trend modeling methodology from Coates and others (2021, 2022a) and incorporates population lek count data for 1960–2024. Included in this report are methodological updates to lek count data aggregation, state-space model forecasting, and targeted annual warning system signals, which are detailed under individual Modification sections. State-space models estimated a 2.9-percent average annual decline in sage-grouse populations between 1966 and 2021 (Period 1, six population oscillations) across their geographical range. The average annual decline among climate clusters for the same number of oscillations ranged between 2.2 and 3.4 percent. Cumulative declines were 41.2, 64.1, and 78.8 percent range-wide in Period 5 (19 years), Period 3 (35 years), and Period 1 (55 years), respectively.

California, Colorado, Idaho, Montana, Nevada, Nort↗
USGS2013

The National Water-Quality Assessment (NAWQA) Program planned monitoring and modeling activities for Texas, 2013–23

The U.S. Geological Survey’s (USGS) National Water-Quality Assessment (NAWQA) Program was established by Congress in 1992 to answer the following question: What is the status of the Nation’s water quality and is it getting better or worse? Since 1992, NAWQA has been a primary source of nationally consistent data and information on the quality of the Nation’s streams and groundwater. Data and information obtained from objective and nationally consistent water-quality monitoring and modeling activities provide answers to where, when, and why the Nation’s water quality is degraded and what can be done to improve and protect it for human and ecosystem needs. For NAWQA’s third decade (2013–23), a new strategic Science Plan has been developed that describes a strategy for building upon and enhancing the USGS’s ongoing assessment of the Nation’s freshwater quality and aquatic ecosystems.

Texas↗
USGS2025

U.S. Geological Survey global seabed mineral resources

The U.S. Geological Survey (USGS) provides science and data on seabed mineral resources and ecosystems, as well as on the potential hazards associated with extraction. The Nation relies on minerals for infrastructure, technology, manufacturing, and energy production. Critical minerals are essential to the economic and national security of the United States and have a supply chain vulnerable to disruption. For decades, USGS scientific innovation has contributed to the delineation of seabed mineral resources, the mechanisms of seabed mineral formation, and the environmental impacts of resource extraction. Since 1962, the USGS has also led scientific inquiries into the potential for deep sea mining. By providing impartial science on seabed minerals and their environmental setting in the deep oceans, the USGS enables decision-makers to evaluate the best practices for mineral resource development.

Fact Sheet↗
USGS2007

International Polar Year (IPY), 2007-2008

The International Polar Year is an intense scientific campaign to explore new frontiers in polar science, improve our understanding of the critical role of the polar regions in global processes, engage the public in polar discovery, and help attract the next generation of earth scientists. Participation in the IPY allows the USGS to celebrate this enduring tradition with the rest of the global polar research community and to renew our commitment to polar science at a time when the world is focused on the extraordinary changes happening in these regions.

General Information Product↗
USGS2008

Quality-Assurance Plan for Water-Quality Activities in the USGS Ohio Water Science Center

In accordance with guidelines set forth by the Office of Water Quality in the Water Resources Discipline of the U.S. Geological Survey, a quality-assurance plan has been written for use by the Ohio Water Science Center in conducting water-quality activities. This quality-assurance plan documents the standards, policies, and procedures used by the Ohio Water Science Center for activities related to the collection, processing, storage, analysis, and publication of water-quality data. The policies and procedures documented in this quality-assurance plan for water-quality activities are meant to complement the Ohio Water Science Center quality-assurance plans for water-quality monitors, the microbiology laboratory, and surface-water and ground-water activities.

Open-File Report↗
USGS2009

U.S. Geological Survey Global Seismographic Network - Five-Year Plan 2006-2010

The Global Seismographic Network provides data for earthquake alerting, tsunami warning, nuclear treaty verification, and Earth science research. The system consists of nearly 150 permanent digital stations, distributed across the globe, connected by a modern telecommunications network. It serves as a multi-use scientific facility and societal resource for monitoring, research, and education, by providing nearly uniform, worldwide monitoring of the Earth. The network was developed and is operated through a partnership among the National Science Foundation (http://www.nsf.gov), the Incorporated Research Institutions for Seismology (http://www.iris.edu/hq/programs/gsn), and the U.S. Geological Survey (http://earthquake.usgs.gov/gsn).

Open-File Report↗
USGS2023

Summary of the history and research of the U.S. Geological Survey gas hydrate properties laboratory in Menlo Park, California, active from 1993 to 2022

The U.S. Geological Survey (USGS) Clathrate Hydrate Properties Project, active from 1993 to 2022 in Menlo Park, California, stemmed from an earlier project on the properties of planetary ices supported by the National Aeronautics and Space Administration’s (NASA’s) Planetary Geology and Geophysics Program. We took a material science approach in both projects, emphasizing chemical purity of samples, having controlled grain size and grain texture, and having verified crystal structures and phase relations. A foundational contribution from our USGS Gas Hydrate Properties Laboratory (GHPL) was in demonstrating the ability to reproducibly create such pure clathrate hydrate samples for study. Clathrate sample synthesis was achieved by heating sieved and weighed pure granular water ice in the presence of cold clathrate-forming gas or liquid. During heating, the ice melts at the grain scale and reacts with the gas to form clathrate. The resulting material has the desired uniformity and purity, with known intergranular porosity; our subsequent measurements showed that these clathrates exhibited the established clathrate structures and phase relations. This novel synthesis method was successful in creating clathrates of pure methane, ethane, propane, carbon dioxide, and multi-component gases. By mixing sand or silt with granular ice, we were also able to make clathrate-sediment aggregates with controlled grain textures. This simple method, adopted by many others in the community, permitted us to measure the physical and chemical properties of well-characterized and well-crystallized clathrates and clathrate/sediment aggregates. At about the same time, we adapted conventional scanning electron microscopy to cryogenic conditions for analysis of grain-scale characteristics of clathrates made in the GHPL as well as those collected from nature by drill core. The uniformity and reproducibility of our samples also allowed us to investigate how clathrates respond to environmental changes in chemistry, temperature, and pressure: we measured chemical exchange rates with dissolved gas species—such as noble gases and chlorofluorocarbons—as well as rates of clathrate dissolution and decomposition. These advances include the first accurate mapping of the conditions that promote the remarkable process of “anomalous preservation” at room pressure, a metastability that offers potential application for low-cost and safe transportation of natural gas from gas fields far from pipelines. Another advancement stemming from the GHPL was the compaction of as-synthesized porous clathrates to nearly full density by applying external pressure using three different techniques. Compaction allows for high-accuracy measurements of many fundamental physical and chemical properties of these materials, such as elastic wavespeeds and moduli, complete thermal properties, decomposition rates, thermal expansion, and clathrate equations of state. These properties and others, in turn, have helped USGS scientists to interpret geophysical well logs and active geophysical surveys, as well as model the rates of gas production from hydrate deposits in nature. Studying this class of icy minerals that occur in abundance on Earth and in the outer solar system has been a fascinating laboratory journey. Here, we summarize the history and major findings of the USGS GHPL in Menlo Park, including both in-house research as well as findings from the synergistic collaborations with other agencies and institutes that were key to the success of our laboratory. The Menlo Park GHPL was more formally incorporated within the USGS Gas Hydrates Project, a collaboration among multiple USGS Science Centers, in the early 2000s under the leadership of Deborah Hutchinson, and now under the leadership of Carolyn Ruppel and Timothy Collett.

Open-File Report↗
USGS2008

Comparison of storage capacity and sedimentation trends of Lago Guayabal, Puerto Rico-December 2001 and October 2006

Lago Guayabal dam is located on the Río Jacaguas in the municipality of Villalba in southern Puerto Rico, about 4 kilometers north of the town of Juana Díaz and about 5 kilometers south of Villalba (fig. 1). The dam is owned and operated by the Puerto Rico Electric Power Authority (PREPA) and was constructed in 1913 for the irrigation of croplands in the southern coastal plains of Puerto Rico. The reservoir impounds the waters of the Río Jacaguas and those of the Río Toa Vaca, when the Toa Vaca dam overflows or releases water. The reservoir has a drainage area of 53.8 square kilometers. The dam is a concrete gravity structure with a normal pool (at top of flashboards) elevation of 103.94 meters above mean sea level (Puerto Rico Electric Power Authority, 1988). During October 2006, the U.S. Geological Survey (USGS), Caribbean Water Science Center, in cooperation with the Puerto Rico Aqueduct and Sewer Authority (PRASA) conducted a bathymetric survey of Lago Guayabal to update the reservoir storage capacity and actualize the reservoir sedimentation rate by comparing the 2006 data with the previous 2001 bathymetric survey results. The purpose of this report is to describe and document the USGS sedimentation survey conducted at Lago Guayabal during October 2006, including the methods used to update the reservoir storage capacity, sedimentation rates, and areas of substantial sediment accumulation since December 2001. The Lago Guayabal sedimentation history up to 2001 was published by the USGS in 2003 (Soler-López, 2003); therefore, this report focuses on the comparison between the 2001 and current bathymetric surveys of Lago Guayabal..

Puerto Rico↗
USGS2026

Correcting a systematic bias in an ocean drilling project site 882 alkenone sea surface temperature record

Reconstructions of sea surface temperature (SST) in the geologic record are fundamental to our understanding of Earth's climate history and the evaluation of Earth's climate sensitivity to greenhouse gas forcing. SSTs are reconstructed with a variety of methods, including alkenone biomarker lipids produced by certain coccolithophore algae. One such alkenone SST reconstruction from the subpolar northwest Pacific Ocean Drilling Program (ODP) Site 882 (50.21° N, 167.35° E, 3244 m water depth) has played a large role in shaping the paleoclimate science community's view of global climate warmth during the Late Pliocene (3.6–2.6 million years ago) and the subsequent cooling that characterized the intensification of Northern Hemisphere Glaciation (Haug, 1995; Haug et al., 2005; Martínez-Garcia et al., 2010). First, we have found that the values reported in the PANGAEA archive for this ODP Site 882 alkenone dataset were inaccurately reported as U K' 37 values when they are instead U K 37 ( https://doi.org/10.1594/PANGAEA.315092 , Haug and Sarnthein, 2005). This error in the archived data table resulted in the incorporation of inaccurate absolute SST estimates by several studies that applied U K' 37 calibrations to this ODP Site 882 dataset (e.g., Brennan et al., 2022; Clark et al., 2024, 2025; Tierney et al., 2019, 2025b). Second, using other published data from ODP Site 882 (Studer et al., 2012) and nearby Site 883 (51.11° N, 167.46° E, 2384 m water depth; Herbert et al., 2016; Novak et al., 2024), we show that the original Haug (1995) alkenone SST record at ODP Site 882 systematically reports an amplified range of absolute SST values compared to the more recently generated data. This observation is consistent with the known concentration-dependent biases of the gas chromatography chemical ionization mass spectrometry (GC-CI-MS) analytical method used by the original ODP Site 882 study (Chaler et al., 2000, 2003; Haug, 1995; Hefter, 2008; Rosell-Mele et al., 1995). These concentration-dependent analytical biases complicate applying a uniform correction to the entire Haug (1995) dataset. However, we are able to leverage the published datasets to propose a correction and quantification of uncertainty for a subset of the Haug (1995) dataset measured at similar on-column analyte abundance. For these samples, we find an average analytical uncertainty equivalent to ±2.05 °C , which is greater than and in addition to the typical ±1.4 °C 1 σ prediction uncertainty of the U K' 37 sea surface temperature proxy. We then discuss the implications of the corrected dataset for our understanding of late Neogene and Quaternary climate in the Kuroshio Extension region.

Climate of the Past↗
USGS2012

Anaerobic oxidation of arsenite by autotrophic bacteria: The view from Mono Lake, California

Introduction The phenomenon of arsenite [As(III)] oxidation by aerobic bacteria was first reported by Green (1918), and the many subsequent discoveries made in this realm, most occurring over the past three decades, are the primary focus of this book. In contrast, the fact that select anaerobes can also achieve this feat was an entirely serendipitous discovery. As often occurs in science, the intended path leading towards a stated goal can take an unexpected turn, ultimately leading to greater rewards than those originally anticipated. The intellectual freedom to meander such a path of curiosity-driven research is a great gift especially when one arrives at an unexpected revelation. It is perhaps the most rewarding aspect of a scientist's career. Such was the case when we first uncovered the phenomenon of anaerobic As(III) oxidation. Our arsenic-related field work focused on Mono Lake, California because of its exceptionally high levels of dissolved inorganic arsenic (~200 μM), and the fact that we had previously isolated two novel species of arsenate [As(V)]-respiring bacteria, Bacillus arseniciselenatis and B. selenitireducens from its bottom sediments(Switzer Blum et al. , 1998). Radiotracer investigations employing 73 As(V) measured high As(V) reductase activity in the anoxic water column of the lake, yielding an estimate that this electron sink could mineralize approximately 8-14% of annual phytoplankton productivity (Oremland et al. , 2000), a value confirmed independently on the basis of mass balance considerations (Hollibaugh et al. , 2005). In both studies both groups also used cultivation-based methods (Most-Probable-Numbers) to estimate the densities of As(V)-respiring bacteria in the anoxic water column, and arrived at similar low but detectable values (e.g. 10 2 -10 3 ml -1 ). The next goal was to determine what taxa of As(V)-respiring prokaryotes were involved in these water-column transformations, using culture-independent analyses (Denaturing Gradient Gel Electrophoresis) of As(V)-amended anoxic bottom water. We had expected to find 16S rRNA gene amplicon sequences similar to those from the bacilli we isolated from the sediments, but instead found a few rather unremarkable amplicons in the Epsilon, Gamma and Delta proteobacteria; yet these incubations showed a complete reduction of the added As(V), caused by sulfide-linked oxidation by resident chemoautotrophs of the Delta-proteobacteria (Hoeft et al. , 2004; Hollibaugh et al. , 2006). This As(V) reductase activity was inhibited by nitrate, while addition of As(III) to nitrate-amended waters resulted in the formation of As(V). This observation led us to conclude that there was anaerobic biological oxidation of As(III) to As(V), linked to the provided nitrate ions (Hoeft et al. , 2002).

California↗