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At least 1,387 records · Page 77Linked to original sources

Experimental infection of bats with Geomyces destructans causes white-nose syndrome

White-nose syndrome (WNS) has caused recent catastrophic declines among multiple species of bats in eastern North America. The disease's name derives from a visually apparent white growth of the newly discovered fungus Geomyces destructans on the skin (including the muzzle) of hibernating bats. Colonization of skin by this fungus is associated with characteristic cutaneous lesions that are the only consistent pathological finding related to WNS. However, the role of G. destructans in WNS remains controversial because evidence to implicate the fungus as the primary cause of this disease is lacking. The debate is fuelled, in part, by the assumption that fungal infections in mammals are most commonly associated with immune system dysfunction. Additionally, the recent discovery that G. destructans commonly colonizes the skin of bats of Europe, where no unusual bat mortality events have been reported, has generated further speculation that the fungus is an opportunistic pathogen and that other unidentified factors are the primary cause of WNS. Here we demonstrate that exposure of healthy little brown bats (Myotis lucifugus) to pure cultures of G. destructans causes WNS. Live G. destructans was subsequently cultured from diseased bats, successfully fulfilling established criteria for the determination of G. destructans as a primary pathogen. We also confirmed that WNS can be transmitted from infected bats to healthy bats through direct contact. Our results provide the first direct evidence that G. destructans is the causal agent of WNS and that the recent emergence of WNS in North America may represent translocation of the fungus to a region with a naive population of animals. Demonstration of causality is an instrumental step in elucidating the pathogenesis and epidemiology of WNS and in guiding management actions to preserve bat populations against the novel threat posed by this devastating infectious disease. ?? 2011 Macmillan Publishers Limited. All rights reserved.

Nature↗

Fluorescent biomarkers demonstrate prospects for spreadable vaccines to control disease transmission in wild bats

Vaccines that autonomously transfer among individuals have been proposed as a strategy to control infectious diseases within inaccessible wildlife populations. However, rates of vaccine spread and epidemiological efficacy in real-world systems remain elusive. Here, we investigate whether topical vaccines that transfer among individuals through social contacts can control vampire bat rabies—a medically and economically important zoonosis in Latin America. Field experiments in three Peruvian bat colonies, which used fluorescent biomarkers as a proxy for the bat-to-bat transfer and ingestion of an oral vaccine, revealed that vaccine transfer would increase population-level immunity up to 2.6 times beyond the same effort using conventional, non-spreadable vaccines. Mathematical models showed that observed levels of vaccine transfer would reduce the probability, size and duration of rabies outbreaks, even at low but realistically achievable levels of vaccine application. Models further predicted that existing vaccines provide substantial advantages over culling bats—the policy currently implemented in North, Central and South America. Linking field studies with biomarkers to mathematical models can inform how spreadable vaccines may combat pathogens of health and conservation concern before costly investments in vaccine design and testing.

Nature Ecology and Evotution↗

Batrachochytrium salamandrivorans (Bsal) not detected in an intensive survey of wild North American amphibians

The salamander chytrid fungus (Batrachochytrium salamandrivorans [Bsal]) is causing massive mortality of salamanders in Europe. The potential for spread via international trade into North America and the high diversity of salamanders has catalyzed concern about Bsal in the U.S. Surveillance programs for invading pathogens must initially meet challenges that include low rates of occurrence on the landscape, low prevalence at a site, and imperfect detection of the diagnostic tests. We implemented a large-scale survey to determine if Bsal was present in North America designed to target taxa and localities where Bsal was determined highest risk to be present based on species susceptibility and geography. Our analysis included a Bayesian model to estimate the probability of occurrence of Bsal given our prior knowledge of the occurrence and prevalence of the pathogen. We failed to detect Bsal in any of 11,189 samples from 594 sites in 223 counties within 35 U.S. states and one site in Mexico. Our modeling indicates that Bsal is highly unlikely to occur within wild amphibians in the U.S. and suggests that the best proactive response is to continue mitigation efforts against the introduction and establishment of the disease and to develop plans to reduce impacts should Bsal establish.

Continental United States↗

Open water dreissenid mussel control projects: Lessons learned from a retrospective analysis

Dreissenid mussels are one of the most problematic aquatic invasive species (AIS) in North America, causing substantial ecological and economic effects. To date, dreissenid mussel control efforts in open water have included physical, biological, and chemical methods. The feasibility of successful dreissenid mussel management or eradication in lakes is relatively undocumented in the freshwater management literature. This review presents information on 33 open water dreissenid mussel control projects in 23 North America lakes. We reviewed data from past dreissenid mussel control projects and identified patterns and knowledge gaps to help inform adaptive management strategies. The three key lessons learned include (1) pre- and post-treatment survey methods that are designed to meet management objectives are beneficial, e.g., by sampling for all life stages and taking into account that no survey method is completely comprehensive; (2) defining the treatment area—particularly ensuring it is sufficiently large to capture all life stages present—is critical to meeting management objectives; and (3) control projects provide an opportunity to collect water chemistry, effects on non-target organisms, and other efficacy-related data that can inform safe and effective adaptive management.

Scientific Reports↗

Conservation endocrinology: A noninvasive tool to understand relationships between carnivore colonization and ecological carrying capacity

Reproductive technology, especially the diagnosis of pregnancy by radioimmunoassay of fecal steroid metabolites, is an important component of captive propagation, but its role in our understanding of ecological interactions and in situ biological restoration has been more limited. Where large herbivores have been 'released' from predation by the extirpation of carnivores, controversy often exists about possible detrimental effects at the ecosystem level A related concern is that the reestablishment of large carnivores may decrease the availability of prey populations for human subsistence. We suggest that pregnancy assays can be a valuable tool to help distinguish between the roles of predation versus food-imposed limitations on population size and their effects on juvenile recruitment in wild species. We explored this issue through analyses of fecal progestagen concentration (FPC) levels to document pregnancy in moose (Alces alces) in the southern Greater Yellowstone Ecosystem, a site where wolves (Canis lupus) and grizzly bears (Ursus arctos) are recolonizing former habitats after an absence of more than 60 years. Pregnancy was clearly discernible (mean FPC for pregnant and nonpregnant females, respectively: 10.60 vs. 2.57 ??g/g; p < 0.0001). Among the potential confounding variables that need to be considered if FPC is applied to ecological and demographic questions are whether baseline values are affected by handling, whether neonate survival has been assessed, and whether sampling efforts are directed at both pregnant and nonpregnant animals. With these issues accounted for, the local moose population experienced juvenile survival rates among the highest in North America. Pregnancy rates, however, dropped from 90% in 1966 to about 75% today, rendering them in the lowest fifteenth percentile among moose populations in North America. Our findings suggest that a relatively low frequency of juvenile moose is not the likely result of predation, and they illustrate how endocrinology can be applied to issues involving reproductive events within an ecological context. They also affirm that noninvasive and generally inexpensive endocrinological procedures will be applicable to understanding interactions between recolonizing predators and prey, an issue that will continue to arise because of global restoration efforts, and to the study of rare ungulates in remote systems where data on reproductive events are difficult to obtain.

Wyoming↗

The effects of fire on avian communities: Spatio-temporal attributes of the literature 19122003

We reviewed the temporal, geographic, and biogeographic distribution, as well as relevant research and publication attributes, of 512 documents addressing the effects of fire on avian communities, to provide an assessment of the scope of this literature and recommendations for future research. We summarized relevant attributes of all documents to identify patterns that were then tested against appropriate null models. Most documents reported on original research, with the literature evenly divided between studies investigating controlled fire and those reporting on uncontrolled wildfires. Conceptual reviews made up the second largest category; methodological reviews, bibliographies, and meta-analyses were rare. Although the literature examined spans nearly a century, most documents were published within the last 15 years, with new literature being added at an increasing rate. However, increases seem to be skewed towards original research at the expense of synthesis. An overwhelming majority of documents were published in peer-reviewed scientific journals and in English. Other important publication outlets included MS and PhD theses and conference proceedings. The spatial distribution of documents by continent and biogeographic domain and division differed significantly from expectations based on land area. Future research on avian community response to fire should focus on (1) continued synthesis, emphasizing methodological reviews, bibliographies, and North America; (2) increasing research efforts in areas currently underrepresented in the literature, including Africa, Asia, and South and Central America; and (3) meta-analyses. ?? 2009 IAWF.

International Journal of Wildland Fire↗

Suturing fragmented landscapes: Mosaic hybrid zones in plants may facilitate ecosystem resiliency

Many widespread plant taxa of western North America have diversified into phenotypically and genetically divergent lineages due to complex biogeographic histories across heterogeneous landscapes. Mosaic hybrid zones can form when geographically co-occurring, yet environmentally distinct, lineages cross-pollinate and form hybrids that occupy unique environmental niches absent of a geographic cline. This expands the total environmental space across which parental and hybrid individuals grow, resulting in larger, less fragmented geographic distributions. Here, we highlight hybridization mosaics across three study systems containing taxa critical to widespread plant communities in western North America: Ericameria nauseosa , Artemisia tridentata , and Sphaeralcea fendleri . The systems contain diverged taxa that co-occur across the landscape and hybridize readily. Hybridization among taxa has facilitated niche expansion into intermediate environments consistent with unique combinations of adaptive genetic variation, creating more continuity within each study system—study systems occupy ~820 to 270,000 km 2 more geographic area by virtue of hybridization. Furthermore, hybrids are predicted to play important roles in future climates, as they may occupy 8 to 475% larger distributions compared to present. Convergent patterns signal mosaic hybridization as an underappreciated mechanism with broad ecological and evolutionary ramifications. Leveraging mosaic hybridization may assist the creation of restoration management plans that aim to mitigate the deleterious effects of habitat fragmentation on ecosystems in the context of climate change.

western United States↗

Global irrigated area map (GIAM), derived from remote sensing, for the end of the last millennium

A Global Irrigated Area Map (GIAM) has been produced for the end of the last millennium using multiple satellite sensor, secondary, Google Earth and groundtruth data. The data included: (a) Advanced Very High Resolution Radiometer (AVHRR) 3-band and Normalized Difference Vegetation Index (NDVI) 10 km monthly time-series for 1997-1999, (b) Syste me pour l'Observation de la Terre Vegetation (SPOT VGT) NDVI 1 km monthly time series for 1999, (c) East Anglia University Climate Research Unit (CRU) rainfall 50km monthly time series for 1961-2000, (d) Global 30 Arc-Second Elevation Data Set (GTOPO30) 1 km digital elevation data of the World, (e) Japanese Earth Resources Satellite-1 Synthetic Aperture Radar (JERS-1 SAR) data for the rain forests during two seasons in 1996 and (f) University of Maryland Global Tree Cover 1 km data for 1992-1993. A single mega-file data-cube (MFDC) of the World with 159 layers, akin to hyperspectral data, was composed by re-sampling different data types into a common 1 km resolution. The MFDC was segmented based on elevation, temperature and precipitation zones. Classification was performed on the segments. Quantitative spectral matching techniques (SMTs) used in hyperspectral data analysis were adopted to group class spectra derived from unsupervised classification and match them with ideal or target spectra. A rigorous class identification and labelling process involved the use of: (a) space-time spiral curve (ST-SC) plots, (b) brightness-greenness-wetness (BGW) plots, (c) time series NDVI plots, (d) Google Earth very-high-resolution imagery (VHRI) 'zoom-in views' in over 11 000 locations, (e) groundtruth data broadly sourced from the degree confluence project (3 864 sample locations) and from the GIAM project (1 790 sample locations), (f) high-resolution Landsat-ETM+ Geocover 150m mosaic of the World and (g) secondary data (e.g. national and global land use and land cover data). Mixed classes were resolved based on decision tree algorithms and spatial modelling, and when that did not work, the problem class was used to mask and re-classify the MDFC, and the class identification and labelling protocol repeated. The sub-pixel area (SPA) calculations were performed by multiplying full-pixel areas (FPAs) with irrigated area fractions (IAFs) for every class. A 28 class GIAM was produced and the area statistics reported as: (a) annualized irrigated areas (AIAs), which consider intensity of irrigation (i.e. sum of irrigated areas from different seasons in a year plus continuous year-round irrigation or gross irrigated areas), and (b) total area available for irrigation (TAAI), which does not consider intensity of irrigation (i.e. irrigated areas at any given point of time plus the areas left fallow but 'equipped for irrigation' at the same point of time or net irrigated areas). The AIA of the World at the end of the last millennium was 467 million hectares (Mha), which is sum of the non-overlapping areas of: (a) 252 Mha from season one, (b) 174 Mha from season two and (c) 41 Mha from continuous year-round crops. The TAAI at the end of the last millennium was 399 Mha. The distribution of irrigated areas is highly skewed amongst continents and countries. Asia accounts for 79% (370 Mha) of all AIAs, followed by Europe (7%) and North America (7%). Three continents, South America (4%), Africa (2%) and Australia (1%), have a very low proportion of the global irrigation. The GIAM had an accuracy of 79-91%, with errors of omission not exceeding 21%, and the errors of commission not exceeding 23%. The GIAM statistics were also compared with: (a) the United Nations Food and Agricultural Organization (FAO) and University of Frankfurt (UF) derived irrigated areas and (b) national census data for India. The relationships and causes of differences are discussed in detail. The GIAM products are made available through a web portal (http://www.iwmigiam.org). ?? 2009 Taylor & Francis.

International Journal of Remote Sensing↗

Floristic and climatic reconstructions of two Lower Cretaceous successions from Peru

Climate during the Early Cretaceous in tropical South America has often been reconstructed as arid. However, some areas seem to have been humid. We reconstructed the floristic composition of two tropical stratigraphic successions in Peru using quantitative palynology (rarefied species richness and abundance), and used the abundance of aridity vs. humidity indicator species to infer the predominant climate conditions of this region. The Berriasian to Hauterivian La Merced succession was dominated by fern spores and was predominantly humid. The Albian Aguas Frias succession yielded rich palynofloras, with 127 species, and also indicates predominantly humid conditions. These results support the hypothesis that the west margin of South America was humid during the Early Cretaceous, thus improving the tropical climate reconstructions during the Cretaceous severe global warming episodes.

Palynology↗

Standard methods for sampling freshwater fishes: Opportunities for international collaboration

With publication of Standard Methods for Sampling North American Freshwater Fishes in 2009, the American Fisheries Society (AFS) recommended standard procedures for North America. To explore interest in standardizing at intercontinental scales, a symposium attended by international specialists in freshwater fish sampling was convened at the 145th Annual AFS Meeting in Portland, Oregon, in August 2015. Participants represented all continents except Australia and Antarctica and were employed by state and federal agencies, universities, nongovernmental organizations, and consulting businesses. Currently, standardization is practiced mostly in North America and Europe. Participants described how standardization has been important for management of long-term data sets, promoting fundamental scientific understanding, and assessing efficacy of large spatial scale management strategies. Academics indicated that standardization has been useful in fisheries education because time previously used to teach how sampling methods are developed is now more devoted to diagnosis and treatment of problem fish communities. Researchers reported that standardization allowed increased sample size for method validation and calibration. Group consensus was to retain continental standards where they currently exist but to further explore international and intercontinental standardization, specifically identifying where synergies and bridges exist, and identify means to collaborate with scientists where standardization is limited but interest and need occur.

Fisheries↗

Susceptibility of Koi and Yellow Perch to infectious hematopoietic necrosis virus by experimental exposure

Infectious hematopoietic necrosis virus (IHNV) is a novirhabdoviral pathogen that originated in western North America among anadromous Pacific salmonids. Severe disease epidemics in the late 1970s resulting from IHNV's invasion into farmed Rainbow Trout Oncorhynchus mykiss in North America, Asia, and Europe emphasized IHNV's ability to adapt to new hosts under varying rearing conditions. Yellow Perch Perca flavescens and Koi Carp Cyprinus carpio (hereafter, “Koi”) are aquaculture-reared fish that are highly valued in sport fisheries and the ornamental fish trade, respectively, but it is unknown whether these fish species are vulnerable to IHNV infection. In this study, we exposed Yellow Perch, Koi, and steelhead (anadromous Rainbow Trout) to IHNV by intraperitoneal injection (10 6 PFU/fish) and by immersion (5.7×10 5 PFU/mL) for 7 h, and monitored fish for 28 d. The extended immersion exposure and high virus concentrations used in the challenges were to determine if the tested fish had any level of susceptibility. After experimental exposure, Yellow Perch and Koi experienced low mortality (<6%) compared with steelhead (>35%). Virus was found in dead fish of all species tested and in surviving Yellow Perch by plaque assay and quantitative reverse transcription polymerase chain reaction (qPCR), with a higher prevalence in Yellow Perch than Koi. Infectious virus was also detected in Yellow Perch out to 5 d after bath challenge. These findings indicate that Yellow Perch and Koi are highly resistant to IHNV disease under the conditions tested, but Yellow Perch are susceptible to infection and may serve as possible virus carriers.

Journal of Aquatic Animal Health↗

The Protected Areas Visitor Impact Management (PAVIM) framework: A simplified process for making management decisions

Ecotourism and protected area visitation in Central and South America have resulted in ecological impacts, which some protected areas managers have addressed by employing visitor impact management frameworks. In this paper, we propose the Protected Area Visitor Impact Management (PAVIM) framework as an alternative to carrying capacity and other frameworks such as Limits of Acceptable Change. We use a set of evaluation criteria to compare the relative positive and negative attributes of carrying capacity, other decision-making frameworks and the new framework, within the context of their actual and potential use in Central and South America. Positive attributes of PAVIM include simplicity, flexibility, cost effectiveness, timeliness and incorporating input from stakeholders and local residents. Negative attributes include diminished objectivity and cultural sensitivity issues. Further research and application of PAVIM are recommended.

Journal of Sustainable Tourism↗

An experimental evaluation of the efficacy of imaging flow cytometry (FlowCam) for detecting invasive Dreissened and Corbiculid bivalve veligers

Zebra ( Dreissena polymorpha ) and quagga ( D. bugensis ) mussels, first introduced from central Asia into the Great Lakes of North America in the late 1980s, have crossed the continental divide and more recently spread across western North America. At the same time, several new technologies have been developed for the early detection of dreissenids, including the FlowCam, a digital imaging-in-flow instrument, intended to detect dreissenid planktonic larvae (veligers). However, the efficacy of this technology has rarely been tested. We experimentally evaluated the FlowCam’s ability to capture identifiable images of quagga mussel veligers under 2 different types of conditions: (i) deionized water, and (ii) Columbia River Basin water (CRBW), including natural sediment and native plankton. We further evaluated the FlowCam’s ability to distinguish between dreissenid veligers and corbiculid veligers (Asian clam, Corbicula fluminea ). We interpret our results to indicate that the FlowCam can consistently detect dreissenid veligers across a range of veliger densities. Moreover, the presence of other plankton and detritus only slightly affected dreissenid detection by the FlowCam. However, the orientation of individual bivalve veligers as they were imaged by the FlowCam precluded specific identification of a substantial proportion (24.8%) of veligers as either dreissenid or corbiculid. We suggest that the FlowCam is an important detection tool best utilized as part of a multifaceted approach, including traditional microscopy and possibly environmental DNA.

Lake and Reservoir Management↗

A new dinosaur ichnotaxon from the Lower Cretaceous Patuxent Formation of Maryland and Virginia

In recent years, numerous dinosaur footprints have been discovered on bedding surfaces within the Lower Cretaceous Patuxent Formation of Maryland and Virginia. Among these, distinctive small tracks that display a combination of small manus with five digit impressions and a relatively much larger pes with four toe impressions evidently were made by animals belonging to the ornithischian family Hypsilophodontidae. These tracks differ from any ornithischian ichnotaxon previously described. We here name them Hypsiloichnus marylandicus and provide a description of their diagnostic characteristics. Although hypsilophodontid skeletal remains have not been found in the Patuxent, their skeletal remains are known from Lower Cretaceous strata of similar age in both western North America and Europe. Therefore, it is not surprising to find that an Early Cretaceous representative of this family also existed in eastern North America.

Maryland, Virginia↗

Invertebrate eggs can fly: Evidence of waterfowl-mediated gene flow in aquatic invertebrates

Waterfowl often have been assumed to disperse freshwater aquatic organisms between isolated wetlands, but no one has analyzed the impact of this transport on the population structure of aquatic organisms. For three cladocerans (Daphnia ambigua, Daphnia laevis, and Sida crystallina) and one bryozoan (Cristatella mucedo), we estimated the genetic distances between populations across North America using sequences of several mitochondrial DNA genes and genotypic frequencies at allozyme and microsatellite loci. Waterfowl movements across North America (estimated from band recovery data) explained a significant proportion of the gene flow occurring between populations across the continent for three of the four species, even after controlling for geographic distances between localities. The fourth species, S. crystallina, has propagules less likely to survive desiccation or ingestion by birds. Differences in the capacity to exploit bird-mediated transport are likely to have important consequences for the ecology of aquatic communities and the spread of invasive species.

American Naturalist↗

Snowpack signals in North American tree rings

Climate change has contributed to recent declines in mountain snowpack and earlier runoff, which in turn has intensified hydrological droughts in western North America. Climate model projections suggest that continued and severe snowpack reductions are expected over the 21st century, with profound consequences for ecosystems and human welfare. Yet the current understanding of trends and variability in mountain snowpack is limited by the relatively short and strongly temperature forced observational record. Motivated by the urgent need to better understand snowpack dynamics in a long-term, spatially coherent framework, here we examine snow-growth relationships in western North American tree-ring chronologies. We present an extensive network of snow-sensitive proxy data to support high space/time resolution paleosnow reconstruction, quantify and interpret the type and spatial density of snow related signals in tree-ring records, and examine the potential for regional bias in the tree-ring based reconstruction of different snow drought types (dry versus warm). Our results indicate three distinct snow-growth relationships in tree-ring chronologies: moisture-limited snow proxies that include a spring temperature signal, moisture-limited snow proxies lacking a spring temperature signal, and energy-limited snow proxies. Each proxy type is based on distinct physiological tree-growth mechanisms related to topographic and climatic site conditions, and provides unique information on mountain snowpack dynamics that can be capitalized upon within a statistical reconstruction framework. This work provides a platform and foundational background required for the accelerated production of high-quality annually-resolved snowpack reconstructions from regional to high (<12 km) spatial scales in western North America, and by extension, will support an improved understanding of the vulnerability of snowmelt-derived water resources to natural variability and future climate warming.

Environmental Research Letters↗

Ecology and management of plague in diverse communities of rodents and fleas

Plague originated in Asia as a flea-borne zoonosis of mammalian hosts. Today, the disease is distributed nearly worldwide. In western United States of America, plague is maintained, transmitted, and amplified in diverse communities of rodents and fleas. We examined flea diversity on three species of prairie dogs ( Cynomys spp., PDs) and six species of sympatric small rodents in Montana and Utah, United States of America. Among 2896 fleas, 19 species were identified; 13 were found on PDs and 9 were found on small rodents. In Montana, three flea species were found on PDs; the three species parasitize PDs and mice. In Utah, 12 flea species were found on PDs; the 12 species parasitize PDs, mice, voles, chipmunks, ground squirrels, rock squirrels, and marmots. Diverse flea communities and their willingness to parasitize many types of hosts, across multiple seasons and habitats, may favor plague maintenance and transmission. Flea parasitism on Peromyscus deer mice varied directly with elevation. Fleas are prone to desiccation, and might prosper at higher, mesic elevations; in addition, Peromyscus nest characteristics may vary with elevation. Effective management of plague is critical. Plague management is probably most effective when encompassing communities of rodents and fleas. Treatment of PD burrows with 0.05% deltamethrin dust, which suppressed fleas on PDs for >365 days, suppressed fleas on small rodents for at least 58 days. At one site, deltamethrin suppressed fleas on small rodents for at least 383 days. By simultaneously suppressing fleas on PDs and small rodents, deltamethrin should promote ecosystem resilience and One Health objectives.

Montana, Utah↗

At the end of the road: Lessons learned from comparing model- and design-based approaches to estimate population sizes of boreal birds in Alberta, Canada

Estimating population abundance is a challenging task complicated by the amount, type, and quality of available data. Conservationists have relied on design-based estimates from Partners in Flight (PIF), which primarily uses roadside data from the North American Breeding Bird Survey (BBS) to estimate populations sizes. However, the BBS was not designed to estimate population sizes. We developed models incorporating land cover and climate variables based on roadside and off-road point-count surveys. We calculated spatially explicit, model-based population estimates for 81 landbird species in Bird Conservation Region 6 in Alberta, Canada, and compared these to PIF estimates. We also developed a framework to evaluate how the differences between the detection distance, time-of-day, roadside count, and habitat representation adjustments explain discrepancies between the two estimators. We showed that the key assumptions of the PIF population size estimator were commonly violated in this region, and the two approaches provided very different population size estimates for most species. The average differences between estimators were explained by differences in the detection distance and time-of-day components, but these adjustments left much unexplained variation among species. Differences in the roadside count and habitat representation components explained most of the among-species variation. The variation caused by these factors was large enough to change the population size ranking of the species. The roadside count bias needs serious attention when roadside surveys are used to extrapolate over off-road areas. Habitat representation bias is likely prevalent in regions sparsely and non-representatively sampled by roadside surveys, such as the boreal region of North America, and thus population size estimates for these regions need to be treated with caution for certain species. Model-based integration of available data sources and additional sampling can contribute towards more accurate population size estimates for conservation in remote areas of North America.

Alberta↗