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At least 1,369 records · Page 76Linked to original sources

Emerging tools for continuous nutrient monitoring networks: Sensors advancing science and water resources protection

Sensors and enabling technologies are becoming increasingly important tools for water quality monitoring and associated water resource management decisions. In particular, nutrient sensors are of interest because of the well-known adverse effects of nutrient enrichment on coastal hypoxia, harmful algal blooms, and impacts to human health. Accurate and timely information on nutrient concentrations and loads is integral to strategies designed to minimize risk to humans and manage the underlying drivers of water quality impairment. Using nitrate sensors as an example, we highlight the types of applications in freshwater and coastal environments that are likely to benefit from continuous, real-time nutrient data. The concurrent emergence of new tools to integrate, manage and share large data sets is critical to the successful use of nutrient sensors and has made it possible for the field of continuous nutrient monitoring to rapidly move forward. We highlight several near-term opportunities for Federal agencies, as well as the broader scientific and management community, that will help accelerate sensor development, build and leverage sites within a national network, and develop open data standards and data management protocols that are key to realizing the benefits of a large-scale, integrated monitoring network. Investing in these opportunities will provide new information to guide management and policies designed to protect and restore our nation’s water resources.

Journal of the American Water Resources Associatio↗

Stakeholder engagement to guide decision-relevant water data delivery

Water resources management and policy making require access to reliable scientific data. However, water managers may need to overcome various obstacles to accessing data. For example, insufficient technological infrastructures, low data literacy, and data format complexities often inhibit data user access. Thus, it is imperative to include stakeholders in the design of data delivery systems. The United States Geological Survey's Water Resources Mission Area is currently developing Integrated Water Availability Assessments (IWAAs) — multi-extent, stakeholder driven, near real-time water availability census and prediction for human and ecological uses. To provide appropriate user accessibility to data delivery systems developed for IWAAs, a user-centered design process including stakeholder focus groups was used to determine potential water data user needs and preferences. Focus groups identified five types of potential users: Public sector water resources managers, Public sector water resources manager data analysts, Industry and private companies, Tribal Nations, and Nonprofit organizations. Different water data user types depended on diverse spatial and temporal scale data. Public sector water resources managers benefitted most from data synthesized into user-friendly platforms and Public sector water resources data analysts preferred easy access to raw data. These findings can support the development of a water data delivery platform that meets a variety of user needs.

Journal of the American Water Resources Associatio↗

Temporal trends in agricultural water use and the relationships to hydroclimatic factors in the High Plains aquifer region

The High Plains aquifer (HPA) is the primary water source for agricultural irrigation in the US Great Plains. The water levels in many locations of the aquifer have declined steadily over the past several decades because the rate of water withdrawals exceeds recharge, which has been a serious concern to the water resources management in the region. We evaluated temporal trends and variations in agricultural water use and hydroclimatic variables including precipitation, air temperature, reference evapotranspiration, runoff, groundwater level, and terrestrial water storage across the HPA region for different periods from 1985 to 2020 at the grid, county, or region scale. The results showed that water withdrawals decreased from 21.3 km 3 /year in 1985 to 18.2 km 3 /year in 2015, while irrigated croplands increased from 71,928 km 2 in 1985 to 78,464 km 2 in 2015 in the entire HPA. The hydroclimatic time-series showed wetting trends in most of the northern HPA, but drying and warming trends in the southern region from 1985 to 2020. The groundwater level time-series indicated flat trends in the north, but significant declining in the central and southern HPA. Trends in irrigation water withdrawals and irrigation area across the HPA were controlled by the advancement of irrigation systems and technologies and the management of sustainable water use, but also were affected by dynamical changes in the hydroclimatic conditions.

Journal of the American Water Resources Associatio↗

Transcriptome resources for the frogs Lithobates clamitans and Pseudacris regilla , emphasizing antimicrobial peptides and conserved loci for phylogenetics

We developed genetic resources for two North American frogs, Lithobates clamitans and Pseudacris regilla , widespread native amphibians that are potential indicator species of environmental health. For both species, mRNA from multiple tissues was sequenced using 454 technology. De novo assemblies with Mira3 resulted in 50 238 contigs (N50 = 687 bp) and 48 213 contigs (N50 = 686 bp) for L. clamitans and P. regilla , respectively, after clustering with CD-Hit-EST and purging contigs below 200 bp. We performed BLASTX similarity searches against the Xenopus tropicalis proteome and, for predicted ORFs, HMMER similarity searches against the Pfam-A database. Because there is broad interest in amphibian immune factors, we manually annotated putative antimicrobial peptides. To identify conserved regions suitable for amplicon resequencing across a broad taxonomic range, we performed an additional assembly of public short-read transcriptome data derived from two species of the genus Rana and identified reciprocal best TBLASTX matches among all assemblies. Although P. regilla , a hylid frog, is substantially more diverged from the ranid species, we identified 56 genes that were sufficiently conserved to allow nondegenerate primer design with Primer3. In addition to providing a foundation for comparative genomics and quantitative gene expression analysis, our results enable quick development of nuclear sequence-based markers for phylogenetics or population genetics.

Molecular Ecology Resources↗

Detecting the movement and spawning activity of bigheaded carps with environmental DNA

Bigheaded carps are invasive fishes threatening to invade the Great Lakes basin and establish spawning populations, and have been monitored using environmental DNA (eDNA). Not only does eDNA hold potential for detecting the presence of species, but may also allow for quantitative comparisons like relative abundance of species across time or space. We examined the relationships among bigheaded carp movement, hydrography, spawning and eDNA on the Wabash River, IN, USA. We found positive relationships between eDNA and movement and eDNA and hydrography. We did not find a relationship between eDNA and spawning activity in the form of drifting eggs. Our first finding demonstrates how eDNA may be used to monitor species abundance, whereas our second finding illustrates the need for additional research into eDNA methodologies. Current applications of eDNA are widespread, but the relatively new technology requires further refinement.

Molecular Ecology Resources↗

Characterization of a Y-specific duplication/insertion of the anti-Mullerian hormone type II receptor gene based on a chromosome-scale genome assembly of yellow perch, Perca flavescens

Yellow perch, Perca flavescens , is an ecologically and economically important species native to a large portion of the northern United States and southern Canada and is also a promising candidate species for aquaculture. However, no yellow perch reference genome has been available to facilitate improvements in both fisheries and aquaculture management practices. By combining Oxford Nanopore Technologies long-reads, 10X Genomics Illumina short linked reads and a chromosome contact map produced with Hi-C, we generated a high-continuity chromosome-scale yellow perch genome assembly of 877.4 Mb. It contains, in agreement with the known diploid chromosome yellow perch count, 24 chromosome-size scaffolds covering 98.8% of the complete assembly (N50 = 37.4 Mb, L50 = 11). We also provide a first characterization of the yellow perch sex determination locus that contains a male-specific duplicate of the anti-Mullerian hormone type II receptor gene ( amhr2by ) inserted at the proximal end of the Y chromosome (chromosome 9). Using this sex-specific information, we developed a simple PCR genotyping assay which accurately differentiates XY genetic males ( amhr2by + ) from XX genetic females ( amhr2by − ). Our high-quality genome assembly is an important genomic resource for future studies on yellow perch ecology, toxicology, fisheries and aquaculture research. In addition, characterization of the amhr2by gene as a candidate sex-determining gene in yellow perch provides a new example of the recurrent implication of the transforming growth factor beta pathway in fish sex determination, and highlights gene duplication as an important genomic mechanism for the emergence of new master sex determination genes.

Molecular Ecology Resources↗

Landscape transcriptomics as a tool for addressing global change effects across diverse species

Landscape transcriptomics is an emerging field studying how genome-wide expression patterns reflect dynamic landscape-scale environmental drivers, including habitat, weather, climate, and contaminants, and the subsequent effects on organismal function. This field is benefitting from advancing and increasingly accessible molecular technologies, which in turn are allowing the necessary characterization of transcriptomes from wild individuals distributed across natural landscapes. This research is especially important given the rapid pace of anthropogenic environmental change and potential impacts that span levels of biological organization. We discuss three major themes in landscape transcriptomic research: connecting transcriptome variation across landscapes to environmental variation, generating and testing hypotheses about the mechanisms and evolution of transcriptomic responses to the environment, and applying this knowledge to species conservation and management. We discuss challenges associated with this approach and suggest potential solutions. We conclude that landscape transcriptomics has great promise for addressing fundamental questions in organismal biology, ecology, and evolution, while providing tools needed for conservation and management of species.

Molecular Ecology Resources↗

A functional model for characterizing long-distance movement behaviour

Advancements in wildlife telemetry techniques have made it possible to collect large data sets of highly accurate animal locations at a fine temporal resolution. These data sets have prompted the development of a number of statistical methodologies for modelling animal movement. Telemetry data sets are often collected for purposes other than fine-scale movement analysis. These data sets may differ substantially from those that are collected with technologies suitable for fine-scale movement modelling and may consist of locations that are irregular in time, are temporally coarse or have large measurement error. These data sets are time-consuming and costly to collect but may still provide valuable information about movement behaviour. We developed a Bayesian movement model that accounts for error from multiple data sources as well as movement behaviour at different temporal scales. The Bayesian framework allows us to calculate derived quantities that describe temporally varying movement behaviour, such as residence time, speed and persistence in direction. The model is flexible, easy to implement and computationally efficient. We apply this model to data from Colorado Canada lynx ( Lynx canadensis ) and use derived quantities to identify changes in movement behaviour.

Methods in Ecology and Evolution↗

Estimating occupancy and abundance using aerial images with imperfect detection

Species distribution and abundance are critical population characteristics for efficient management, conservation, and ecological insight. Point process models are a powerful tool for modelling distribution and abundance, and can incorporate many data types, including count data, presence-absence data, and presence-only data. Aerial photographic images are a natural tool for collecting data to fit point process models, but aerial images do not always capture all animals that are present at a site. Methods for estimating detection probability for aerial surveys usually include collecting auxiliary data to estimate the proportion of time animals are available to be detected. We developed an approach for fitting point process models using an N -mixture model framework to estimate detection probability for aerial occupancy and abundance surveys. Our method uses multiple aerial images taken of animals at the same spatial location to provide temporal replication of sample sites. The intersection of the images provide multiple counts of individuals at different times. We examined this approach using both simulated and real data of sea otters ( Enhydra lutris kenyoni ) in Glacier Bay National Park, southeastern Alaska. Using our proposed methods, we estimated detection probability of sea otters to be 0.76, the same as visual aerial surveys that have been used in the past. Further, simulations demonstrated that our approach is a promising tool for estimating occupancy, abundance, and detection probability from aerial photographic surveys. Our methods can be readily extended to data collected using unmanned aerial vehicles, as technology and regulations permit. The generality of our methods for other aerial surveys depends on how well surveys can be designed to meet the assumptions of N -mixture models.

Methods in Ecology and Evolution↗

A new framework for analysing automated acoustic species detection data: Occupancy estimation and optimization of recordings post-processing

The development and use of automated species-detection technologies, such as acoustic recorders, for monitoring wildlife are rapidly expanding. Automated classification algorithms provide a cost- and time-effective means to process information-rich data, but often at the cost of additional detection errors. Appropriate methods are necessary to analyse such data while dealing with the different types of detection errors. We developed a hierarchical modelling framework for estimating species occupancy from automated species-detection data. We explore design and optimization of data post-processing procedures to account for detection errors and generate accurate estimates. Our proposed method accounts for both imperfect detection and false positive errors and utilizes information about both occurrence and abundance of detections to improve estimation. Using simulations, we show that our method provides much more accurate estimates than models ignoring the abundance of detections. The same findings are reached when we apply the methods to two real datasets on North American frogs surveyed with acoustic recorders. When false positives occur, estimator accuracy can be improved when a subset of detections produced by the classification algorithm is post-validated by a human observer. We use simulations to investigate the relationship between accuracy and effort spent on post-validation, and found that very accurate occupancy estimates can be obtained with as little as 1% of data being validated. Automated monitoring of wildlife provides opportunity and challenges. Our methods for analysing automated species-detection data help to meet key challenges unique to these data and will prove useful for many wildlife monitoring programs.

Methods in Ecology and Evolution↗

Modelling sound attenuation in heterogeneous environments for improved bioacoustic sampling of wildlife populations

Acoustic sampling methods are becoming increasingly important in biological monitoring. Sound attenuation is one of the most important dynamics affecting the utility of acoustic data as it directly affects the probability of detection of individuals by acoustic sensor arrays and especially the localization of acoustic signals necessary in telemetry studies. Therefore, models of sound attenuation are necessary to make efficient use of acoustic data in ecological monitoring and assessment applications. Models of attenuation in widespread use are based on Euclidean distance between source and sensor, which is justified under spherical attenuation of sound waves in homogeneous environments. In this paper, I develop a model of sound attenuation based on a non‐Euclidean cost‐weighted distance metric which contains attenuation coefficients that characterize the attenuation of sound due to environmental heterogeneity in the vicinity of an acoustic sensor array. I show that parameters of the proposed attenuation model can be estimated by maximum likelihood using experimental data from an array of fixed sources, thus allowing investigators who use bioacoustic methods to devise explicit models of sound attenuation in situ and apply them to localization of sources and density estimation. In addition, drawing on analogy with spatial capture–recapture models, I argue that parameters of the non‐Euclidean model of attenuation can be estimated when source locations are unknown . Thus, the models can be applied to real field studies which require estimation of attenuation parameters or localization of signals. Models of heterogeneous sound attenuation allow more accurate descriptions of acoustic monitoring data, and therefore should produce more accurate estimates of ecological parameters of interest, including source locations, density, and movement trajectories. Moreover, the ability to test specific hypotheses about the effects of habitat and landscape structure on sound attenuation can improve the design of acoustic monitoring arrays and lead to more efficient deployment of acoustic sensing technology.

Methods in Ecology and Evolution↗

Occupancy models for citizen-science data

Large‐scale citizen‐science projects, such as atlases of species distribution, are an important source of data for macroecological research, for understanding the effects of climate change and other drivers on biodiversity, and for more applied conservation tasks, such as early‐warning systems for biodiversity loss. However, citizen‐science data are challenging to analyse because the observation process has to be taken into account. Typically, the observation process leads to heterogeneous and non‐random sampling, false absences, false detections, and spatial correlations in the data. Increasingly, occupancy models are being used to analyse atlas data. We advocate a dual approach to strengthen inference from citizen science data for the questions the programme is intended to address: (a) the survey design should be chosen with a particular set of questions and associated analysis strategy in mind and (b) the statistical methods should be tailored not only to those questions but also to the specific characteristics of the data. We review the consequences of particular survey design choices that typically need to be made in atlas‐style citizen‐science projects. These include spatial resolution of the sampling units, allocation of effort in space, and collection of information about the observation process. On the analysis side, we review extensions of the basic occupancy models that are frequently necessary with atlas data, including methods for dealing with heterogeneity, non‐independent detections, false detections, and violation of the closure assumption. New technologies, such as cell‐phone apps and fixed remote detection devices, are revolutionizing citizen‐science projects. There is an opportunity to maximize the usefulness of the resulting datasets if the protocols are rooted in robust statistical designs and data analysis issues are being considered. Our review provides guidelines for designing new projects and an overview of the current methods that can be used to analyse data from such projects.

Methods in Ecology and Evolution↗

Migration Mapper: Identifying movement corridors and seasonal ranges for large mammal conservation

Modern tracking technology has facilitated a novel understanding of terrestrial mammal movement while revealing that movements are being truncated and lost. The first step towards conserving mobile animals is identifying movement corridors and key seasonal ranges. Yet, the identification and subsequent mapping of these important areas has remained a challenge due to the analytical skills necessary to conduct such analyses. Migration Mapper (MM) is a user-friendly software that provides tools to analyse global positioning system (GPS) collar data to create season-specific, population-level polygons representing areas where most of a population moves (i.e. movement corridors) and areas where most of a population spends time (e.g. high-use areas, seasonal ranges). MM consists of six standalone modules including data cleaning and review, seasonal movement delineation, movement model application, calculation of population-level outputs and visualization of results. Analysis of GPS data using MM can provide the spatial polygons necessary to facilitate conservation and policy planning. New initiatives at the local and global levels are already beginning to use MM to facilitate conservation of large, terrestrial mammals.

Methods in Ecology and Evolution↗

A hierarchical modelling framework for estimating individual- and population-level reproductive success from movement data

Rapidly advancing animal telemetry technologies paired with new statistical models can provide insight into the behaviour of otherwise unobservable free-living animals. Changes in behaviour apparent from pairing telemetry with statistical models often occur as animals undertake key life-history activities, such as reproduction. For many species that are secretive or occupy remote areas, these life-history events are difficult to detect with conventional survey techniques, and consequently, vital rates are difficult to estimate. We present a hierarchical modelling framework, which integrates movement data observed via animal-borne telemetry and optional, infrequent survey data, to estimate individual- and population-level reproductive success. The approach combines a mechanistic movement model and survival model, and allows for assessing the effects of hypothesized individual and environmental covariates on reproductive success. We first tested our approach with simulated data, and then applied it to movement data from migratory golden eagles ( Aquila chrysaetos) breeding in southcentral Alaska across four breeding seasons. We show that results supported our biological hypotheses that changes in movement coincided with the timing of reproductive failures, and that changes in movement could be used to assess breeding success (and failure) at the individual and population levels. The analysis also provided evidence of inter-annual variation in population-level nest success and the timing of nesting failures. This new approach is adaptable to many species that care for young and can be tracked with telemetry devices, and can provide not only individual-level information useful for testing ecological hypotheses, but estimates of demographic parameters that can directly inform conservation and management if tagged animals are representative of the population.

Methods in Ecology and Evolution↗

A transferable approach for quantifying benthic fish sizes and densities in annotated underwater images

1. Benthic fishes are a common target of scientific monitoring but are difficult to quantify because of their close association to bottom habitats that are hard to access. Advances in image-acquisition technologies, machine vision, and deep learning have made capturing and quantifying fishes with cameras increasingly feasible. We present a method and open-source software called ‘FishScale’ to estimate benthic fish lengths, numeric abundance, and biomass density in underwater environments assessed with down-looking monocular images. 2. ‘FishScale’ estimates fish abundances and size frequencies from near-nadir monocular images where fish have already been semantically segmented. The software accounts for lens distortion, underwater magnification effects, and fish body curvature to automatically estimate fish lengths and the areas of images where they were captured. Numeric and biomass density are estimated through a deterministic machine vision algorithm that requires a user-provided length-weight relationship for species of interest and calibration images. 3. Results from validation studies show that lengths and weights can be estimated with high accuracy and precision for round goby ( Neogobius melanostomus ) captured in distorted action camera images, and from large-bodied lake trout ( Salvelinus namaycush ) imaged with a machine vision camera. The real-world utility of the approach is demonstrated in a case study estimating round goby abundances and size frequencies along a 10.7-km transect surveyed with an autonomous underwater vehicle in Lake Michigan, USA. 4. Our validation studies demonstrate that the approach estimates benthic and benthopelagic fish lengths and weights with little bias and good accuracy and precision for species with much different body shapes and sizes. The method is applicable to data collected using a variety of nadir imaging approaches with widespread applications to fisheries monitoring and quantification of any species or object for which nadir images and working distances between the camera and feature of interest are available.

Illinois, Indiana, Michigan, Wisconsin↗

Trading off hatching success and cost in the captive breeding of Whooping Cranes

Captive breeding is an increasingly used conservation strategy for species with a high risk of extinction in the wild, but managing a captive breeding programme can be challenging if there is a deficiency in knowledge about the species’ breeding biology. A knowledge gap can make it difficult to evaluate different management options. For avian species, egg hatching success is a key demographic parameter, and data-logging egg technology can provide important information on optimal species-specific incubation conditions, which can help inform captive breeding practises and identify efficient captive management options. In the context of a captive breeding programme for endangered Whooping Cranes Grus americana , we investigated associations between hatching success and incubation conditions, including environmental parameters (temperature, relative humidity and egg turning rate), and incubation type (artificial incubation; foster incubation by Sandhill Cranes, Grus canadensis ; and Whooping Crane incubation). Finally, we considered both cost and breeding output in an analysis of incubation practises. We found that daily mean temperatures were negatively associated with hatching success, and that hatching success was highest with incubation under Sandhill Cranes. However, incubation by artificial incubators, rather than Sandhill Cranes, provided a trade-off between cost and breeding output that is likely to be acceptable to many captive programme managers. We encourage other captive breeding programmes to use innovations that help to increase potential release numbers for conservation translocations by considering biological and financial constraints.

Animal Conservation↗

Climate change and alpine stream biology: progress, challenges, and opportunities for the future

In alpine regions worldwide, climate change is dramatically altering ecosystems and affecting biodiversity in many ways. For streams, receding alpine glaciers and snowfields, paired with altered precipitation regimes, are driving shifts in hydrology, species distributions, basal resources, and threatening the very existence of some habitats and biota. Alpine streams harbour substantial species and genetic diversity due to significant habitat insularity and environmental heterogeneity. Climate change is expected to affect alpine stream biodiversity across many levels of biological resolution from micro- to macroscopic organisms and genes to communities. Herein, we describe the current state of alpine stream biology from an organism-focused perspective. We begin by reviewing seven standard and emerging approaches that combine to form the current state of the discipline. We follow with a call for increased synthesis across existing approaches to improve understanding of how these imperiled ecosystems are responding to rapid environmental change. We then take a forward-looking viewpoint on how alpine stream biologists can make better use of existing data sets through temporal comparisons, integrate remote sensing and geographic information system (GIS) technologies, and apply genomic tools to refine knowledge of underlying evolutionary processes. We conclude with comments about the future of biodiversity conservation in alpine streams to confront the daunting challenge of mitigating the effects of rapid environmental change in these sentinel ecosystems.

Biological Reviews↗

Riverscape approaches in practice: Perspectives and applications

Landscape perspectives in riverine ecology have been undertaken increasingly in the last 30 years, leading aquatic ecologists to develop a diverse set of approaches for conceptualizing, mapping and understanding ‘riverscapes’. Spatiotemporally explicit perspectives of rivers and their biota nested within the socio-ecological landscape now provide guiding principles and approaches in inland fisheries and watershed management. During the last two decades, scientific literature on riverscapes has increased rapidly, indicating that the term and associated approaches are serving an important purpose in freshwater science and management. We trace the origins and theoretical foundations of riverscape perspectives and approaches and examine trends in the published literature to assess the state of the science and demonstrate how they are being applied to address recent challenges in the management of riverine ecosystems. We focus on approaches for studying and visualizing rivers and streams with remote sensing, modelling and sampling designs that enable pattern detection as seen from above (e.g. river channel, floodplain, and riparian areas) but also into the water itself (e.g. aquatic organisms and the aqueous environment). Key concepts from landscape ecology that are central to riverscape approaches are heterogeneity, scale (resolution, extent and scope) and connectivity (structural and functional), which underpin spatial and temporal aspects of study design, data collection and analysis. Mapping of physical and biological characteristics of rivers and floodplains with high-resolution, spatially intensive techniques improves understanding of the causes and ecological consequences of spatial patterns at multiple scales. This information is crucial for managing river ecosystems, especially for the successful implementation of conservation, restoration and monitoring programs. Recent advances in remote sensing, field-sampling approaches and geospatial technology are making it increasingly feasible to collect high-resolution data over larger scales in space and time. We highlight challenges and opportunities and discuss future avenues of research with emerging tools that can potentially help to overcome obstacles to collecting, analysing and displaying these data. This synthesis is intended to help researchers and resource managers understand and apply these concepts and approaches to address real-world problems in freshwater management.

Biological Reviews↗