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Genetic integrity, population status, and long-term viability of isolated populations of shoal bass in the upper Chattahoochee River basin, Georgia

This report characterizes the status of multiple isolated Shoal Bass ( Micropterus cataractae ) populations in the upper Chattahoochee River basin (UCRB), Georgia. The Shoal Bass, a sport fish endemic to the Apalachicola-Chattahoochee-Flint River (ACF) basin, is a fluvial-specialist species considered vulnerable to local extirpations and extinction due to habitat fragmentation and introgression with non-native congeners. Perhaps one of the most isolated populations of Shoal Bass exists in a 2-km reach of Big Creek, a tributary of the Chattahoochee River located near Roswell, Georgia. Big Creek is partially contained within the Chattahoochee River National Recreation Area, although the Big Creek watershed is riddled with urban land cover. Roswell Mill Dam limits the upstream extent of the Shoal Bass population at Big Creek, and the downstream extent is presumably limited to the confluence of Big Creek and the Chattahoochee River. This reach of the Chattahoochee River is thermally depressed because of coldwater releases from Lake Lanier, and is considered unsuitable for Shoal Bass. Herein, we examine the genetic integrity, population status, and long-term viability of the Shoal Bass population in Big Creek. We also examine two additional Shoal Bass populations that occur in the UCRB, specifically the Chestatee River and the upper Chattahoochee River, both of which are impounded at Lake Lanier. Together, the Shoal Bass inhabiting these three stream systems comprise a distinct genetic stock of Shoal Bass (Taylor 2017), underscoring the importance of conserving these populations towards maintaining the overall diversity and adaptive potential of the species. We assessed genetic diversity and estimated effective population sizes within these three rivers by genotyping fish with 16 microsatellite DNA markers. Results demonstrated that the Shoal Bass population in Big Creek has experienced high rates of introgression with non-native Smallmouth Bass (M. dolomieu), purportedly introduced into the Chattahoochee River in the past 10-15 years. Alarmingly, only 24% (15 of 62) of putative Shoal Bass collected from Big Creek were genetically pure Shoal Bass, whereas the majority of fish were first-filial (F1) generation hybrids and unidirectional backcrosses towards Shoal Bass. Fleeting opportunity may remain to conserve the native genome of the Shoal Bass population in Big Creek. High hybridization rates prevented genetic diversity analysis for the Big Creek population. Shoal Bass populations in the Chestatee and Chattahoochee rivers displayed levels of genetic diversity similar to populations that persist in other rivers in the ACF basin, namely the Flint and Chipola rivers. Effective population sizes of 93.8– 197.4 for the Chestatee and Chattahoochee rivers (combined) suggest that the conservation status of these populations is stable for the short-term, but may be at risk of losing genetic diversity and adaptive potential in the long-term. To estimate age and mortality of the three populations, we used fish scales and capture-markrecapture (CMR) as complementary, non-lethal methods for age estimation. Estimated ages of phenotypic Shoal Bass ranged from 1-12 years in all three populations, demonstrating increased longevity compared to populations elsewhere within the native range. Catch-curve estimates of annual mortality ranged from 18.4-23.7%, which are markedly lower than those observed in other Shoal Bass populations in the ACF basin. These differences in life-history characteristics underscore the need for the development of population-specific management and conservation strategies for Shoal Bass in the UCRB. The lowest recruitment variability (i.e., the variation in year-class strength) was observed in the Chestatee River, a forested watershed, whereas the highest variability was observed in Big Creek, an urbanized watershed. Recruitment strength in Big Creek was negatively influenced by discharge variability in the summer months, suggesting that flashy, sediment-laden flows hinder survival of recently hatched young. Other statistically significant models from Big Creek and the Chattahoochee River indicated that over-winter survival could be an important pinch-point for recruitment in UCRB populations. A multi-agency sampling effort was conducted from May 2013-May 2016 to estimate the population size of Shoal Bass occupying the 1-km of wadeable shoal habitats in Big Creek. Using CMR models, we estimated that approximately 219-348 Shoal Bass (≥ 70 mm total length) occupied the area throughout the duration of our study. These estimates largely reflect abundance of individuals aged 0-2 years, as only 9% (36 of 408) tagged fish were aged ≥ 7 years. Local abundance appeared similar to that reported for a population that inhabited Little Uchee Creek, a similar-sized tributary of the Chattahoochee River, prior to its recent functional extirpation. The low abundance of large, adult Shoal Bass further suggests the long-term viability of the Big Creek population may be in jeopardy. Perhaps most importantly, CMR estimates reflect abundance of phenotypic Shoal Bass – genetic analyses suggest the abundance of pure Shoal Bass could be an order of magnitude smaller. To evaluate the potential for adult Shoal Bass to emigrate from Big Creek into the mainstem Chattahoochee River, we tagged eight adults with acoustic telemetry tags and assessed their seasonal residency at two stationary receiver locations located in increasing proximity to the confluence with the Chattahoochee River. Fish took up residency near the confluence during the fall and winter months, during which time water temperatures in Big Creek were periodically colder than the Chattahoochee River. Although we were unable to document emigration, we conclude that the potential for emigration is highest during the winter months when the Chattahoochee River may be warmer than Big Creek. Two of the tagged fish were caught by anglers near the confluence, suggesting that angling pressure at Big Creek may be higher than previously suspected. Overall, this study observed unique life-history characteristics and characterized the population status of multiple Shoal Bass populations in the UCRB. Populations in the Chestatee and Chattahoochee rivers appear stable at present and likely represent the last remaining strongholds for pure Shoal Bass in the UCRB. Efforts to preserve forested watershed conditions, natural hydrology, and shoal habitats would contribute to the long-term persistence of Shoal Bass populations in these two rivers. Additionally, the detection of non-native Alabama Bass and their associated hybrids in both rivers is cause for concern. Diligent monitoring of hybridization dynamics between Alabama Bass and Shoal Bass is warranted, along with an assessment of Alabama Bass invasion extent upstream of Lake Lanier. The Shoal Bass population in Big Creek is threatened by elevated levels of introgression with nonnative Smallmouth Bass, recruitment variability, low abundance of adults, and isolation from other populations. Conservation intervention is urgently needed to restore and preserve this genetically distinct population, which would contribute to preservation of range wide genetic diversity and adaptability of the species. Additionally, an urban sport fishery for Shoal Bass at Big Creek has the potential to serve as a tool for increasing public awareness, engagement, and support of Shoal Bass conservation efforts in the UCRB. We suggest strategies for conservation of the remnant shoal habitats and Shoal Bass population in Big Creek, including potential development of a supplemental stocking program, selective removal of non-native congeners, and delivery of environmental education programs that could bolster awareness and appreciation.

Georgia↗

Effects of carbamazepine to visual function in early life stage fish

The frequent detection of pharmaceuticals and personal care products (PPCPs) in the environment raises concern for aquatic systems. Carbamazepine (CBZ), an antiepileptic drug, is among the most detected PPCP globally, with concentrations in surface water exceeding those that induce toxicity to aquatic organisms. Non-targeted transcriptomic profiling was conducted in zebrafish ( Danio rerio ) larvae exposed to 0, 1, 5, 10, or 50 μg/L CBZ from 2 h post fertilization (hpf) through hatching, and then sampled at 48, 72, or 144 hpf. Transcriptomic profiles were annotated and characterized with in silico bioinformatic software to assess top enriched pathways and identify targets of environmentally relevant concentrations of CBZ and anchor molecular effects to higher levels of biological organization. Based on this analysis, CBZ was predicted to impair visual perception and sensory system development. The number of eye saccades, determined with a visually mediated behavioral assay, optokinetic response, was significantly reduced in 144 hpf larvae exposed to concentrations as low as 1 μg/L CBZ. These results indicate that environmentally relevant concentrations of CBZ may target and impact processes involved in visual function in fish.

Environmental Research↗

Developing landscape-indicator models for pesticides and nutrients in streams of the Mid-Atlantic Coastal Plain

Collaborative research between the U.S. Environmental Protection Agency (USEPA), Landscape Ecology Branch, and the U.S. Geological Survey (USGS) began in 1999 to relate land use, geology, and other geographic variables to water quality and aquatic ecology in small streams of the Mid-Atlantic Coastal Plain. Results of the study will include landscape-indicator models (see inset) for stream ecological condition and for concentrations of pesticides and nutrients in ground water discharging to small streams. A base network of 174 small (typically first-order) streams was designed across a gradient of hydrogeologic and land-use settings. Additional sites were selected to represent natural watershed conditions and to relate results from the base network to downstream conditions and seasonal hydrologic variability. Benthic-community and habitat assessments were conducted at each stream; water samples from all streams were analyzed for selected pesticides, pesticide metabolites, nutrients, and major ions.

Fact Sheet↗

Comparative morphology of freshwater sculpin inhabiting different environmental conditions in the Chesapeake Bay headwaters

We compared body morphology of two freshwater sculpin taxa that inhabit distinct environmental conditions in the Chesapeake Bay watershed of eastern North America: Potomac sculpin ( C. girardi , Robins; PS) and checkered sculpin ( C. sp. cf. girardi ; CS). Both taxa are endemic to the study area, but PS are more broadly distributed than CS which are limited to karst groundwater-dominated streams in the central Potomac River basin. We examined preserved specimens from sites encompassing their geographic range (six sites per taxon) to evaluate taxonomic differences and environmental effects. Pelvic fin ray counts and body shape distinguished the study taxa. Morphological variation exhibited stronger relationships to environmental covariates (site elevation and basin size) in PS than CS as expected. In addition, the frequency of specimens with a united median chin pore increased with site elevation in PS (but not CS), suggesting thermal effects on preoperculomanibular canal development. However, contrary to expectation, PS did not exhibit greater among-population variation in body shape than CS, and this indicates the potential importance of unmeasured environmental differences among karst groundwater-dominated streams in the study area. Our study also indicated the utility of stream-level management units for CS, an undescribed species recognized as “critically imperiled” by state agencies.

Chesapeake Bay↗

Opportunities for improved consideration of cultural benefits in environmental decision-making

Many cultural benefits of ecosystem services (ES) are difficult to capture in standard ES assessments. Scholars and practitioners often respond to this gap by seeking to develop new scientific methods to capture and integrate the plural values associated with diverse cultural benefits categories. This increasing emphasis on value pluralism represents an essential step toward recognitional justice within ES theory and practice. However, current approaches continue to rest on the assumption that ES-knowledge is only made available to decision-makers through scientific documentation. As a result, scholars and decision-makers fail to account for the role of knowledge pluralism as a core element of recognitional justice, and a key enabling factor for meaningful consideration of the plural values linked to cultural benefits of ES. In this paper, we contribute to a pluralist theory of cultural-benefits-knowledge, and ES-knowledge more broadly. Using a critical interpretive synthesis of environmental management literature, we conceptualize a wider range of knowledge forms that convey cultural benefits, based on the knowledge-as-practice concept in addition to the knowledge-as-product concept more familiar to Western actors. As part of the synthesis, we explore when and how diverse forms of cultural-benefits-knowledge intersect with decision-making processes, and the value aspects and categories of cultural benefits most frequently conveyed by each form of knowledge. Our synthesizing argument offers a critique of the concept of “ES-knowledge-use,” proposing a shift in focus toward “learning opportunities” that exist across phases of decision-making. We demonstrate that attention to a greater diversity of knowledge forms (knowledge pluralism), and a fuller spectrum of opportunities to integrate them (learning opportunities) can support more meaningful consideration of the plural values associated with cultural benefits of ES (value pluralism). In combination, attention to knowledge pluralism and value pluralism can help bring the ES approach into alignment with environmental justice through the recognition and legitimization of multiple identities, well-beings, and human-nature relationships, as reflected in meaningful consideration of the diverse cultural benefits of ES.

Ecosystem Services↗

Identifying biotic integrity and water chemistry relations in nonwadeable rivers of Wisconsin: Toward the development of nutrient criteria

We sampled 41 sites on 34 nonwadeable rivers that represent the types of rivers in Wisconsin, and the kinds and intensities of nutrient and other anthropogenic stressors upon each river type. Sites covered much of United States Environmental Protection Agency national nutrient ecoregions VII-Mostly Glaciated Dairy Region, and VIII-Nutrient Poor, Largely Glaciated upper Midwest. Fish, macroinvertebrates, and three categories of environmental variables including nutrients, other water chemistry, and watershed features were collected using standard protocols. We summarized fish assemblages by index of biotic integrity (IBI) and its 10 component measures, and macroinvertebrates by 2 organic pollution tolerance and 12 proportional richness measures. All biotic and environmental variables represented a wide range of conditions, with biotic measures ranging from poor to excellent status, despite nutrient concentrations being consistently higher than reference concentrations reported for the regions. Regression tree analyses of nutrients on a suite of biotic measures identified breakpoints in total phosphorus (~0.06 mg/l) and total nitrogen (~0.64 mg/l) concentrations at which biotic assemblages were consistently impaired. Redundancy analyses (RDA) were used to identify the most important variables within each of the three environmental variable categories, which were then used to determine the relative influence of each variable category on the biota. Nutrient measures, suspended chlorophyll a, water clarity, and watershed land cover type (forest or row-crop agriculture) were the most important variables and they explained significant amounts of variation within the macroinvertebrate (R 2 = 60.6%) and fish (R 2 = 43.6%) assemblages. The environmental variables selected in the macroinvertebrate model were correlated to such an extent that partial RDA analyses could not attribute variation explained to individual environmental categories, assigning 89% of the explained variation to interactions among the categories. In contrast, partial RDA attributed much of the explained variation to the nutrient (25%) and other water chemistry (38%) categories for the fish model. Our analyses suggest that it would be beneficial to develop criteria based upon a suite of biotic and nutrient variables simultaneously to deem waters as not meeting their designated uses. ?? 2007 Springer Science+Business Media, LLC.

Environmental Management↗

Comparative endocrinology in the 21st century

Hormones coordinate developmental, physiological, and behavioral processes within and between all living organisms. They orchestrate and shape organogenesis from early in development, regulate the acquisition, assimilation, and utilization of nutrients to support growth and metabolism, control gamete production and sexual behavior, mediate organismal responses to environmental change, and allow for communication of information between organisms. Genes that code for hormones; the enzymes that synthesize, metabolize, and transport hormones; and hormone receptors are important targets for natural selection, and variation in their expression and function is a major driving force for the evolution of morphology and life history. Hormones coordinate physiology and behavior of populations of organisms, and thus play key roles in determining the structure of populations, communities, and ecosystems. The field of endocrinology is concerned with the study of hormones and their actions. This field is rooted in the comparative study of hormones in diverse species, which has provided the foundation for the modern fields of evolutionary, environmental, and biomedical endocrinology. Comparative endocrinologists work at the cutting edge of the life sciences. They identify new hormones, hormone receptors and mechanisms of hormone action applicable to diverse species, including humans; study the impact of habitat destruction, pollution, and climatic change on populations of organisms; establish novel model systems for studying hormones and their functions; and develop new genetic strains and husbandry practices for efficient production of animal protein. While the model system approach has dominated biomedical research in recent years, and has provided extraordinary insight into many basic cellular and molecular processes, this approach is limited to investigating a small minority of organisms. Animals exhibit tremendous diversity in form and function, life-history strategies, and responses to the environment. A major challenge for life scientists in the 21st century is to understand how a changing environment impacts all life on earth. A full understanding of the capabilities of organisms to respond to environmental variation, and the resilience of organisms challenged by environmental changes and extremes, is necessary for understanding the impact of pollution and climatic change on the viability of populations. Comparative endocrinologists have a key role to play in these efforts.

Integrative and Comparative Biology↗

Isotopes as indicators of environmental change

In addition to providing an understanding of processes within a catchment system, isotopic techniques have been instrumental in providing reconstructions of catchment climate and other environmental indicators at various time scales. Many recent changes are a direct consequence of anthropogenic activities. Isotopic analysis serves as a valuable tool for distinguishing between natural variations in long-term climatic patterns and anthropogenic effects, yielding improved understanding of natural feedback mechanisms and the development of realistic remediation strategies. This chapter discusses the examples of isotopic techniques that have been applied to understand several types of ongoing and recent environmental changes, and in paleo-environmental studies. It discusses isotope geochemistry, hydrology, and climatology to look at new ways of applying isotopic tracing techniques to provide information on environmental change. It also gives an overview on how isotopic indicators are being applied in investigations of environmental change in continental settings.

Book chapter↗

CRMS vegetation analytical team framework: Methods for collection, development, and use of vegetation response variables

This document identifies the main objectives of the Coastwide Reference Monitoring System (CRMS) vegetation analytical team, which are to provide (1) collection and development methods for vegetation response variables and (2) the ways in which these response variables will be used to evaluate restoration project effectiveness. The vegetation parameters (that is, response variables) collected in CRMS and other coastal restoration projects funded under the Coastal Wetlands Planning, Protection and Restoration Act (CWPPRA) are identified, and the field collection methods for these parameters are summarized. Existing knowledge on community and plant responses to changes in environmental drivers (for example, flooding and salinity) from published literature and from the CRMS and CWPPRA monitoring dataset are used to develop a suite of indices to assess wetland condition in coastal Louisiana. Two indices, the floristic quality index (FQI) and a productivity index, are described for herbaceous and forested vegetation. The FQI for herbaceous vegetation is tested with a long-term dataset from a CWPPRA marsh creation project. Example graphics for this index are provided and discussed. The other indices, an FQI for forest vegetation (that is, trees and shrubs) and productivity indices for herbaceous and forest vegetation, are proposed but not tested. New response variables may be added or current response variables removed as data become available and as our understanding of restoration success indicators develops. Once indices are fully developed, each will be used by the vegetation analytical team to assess and evaluate CRMS/CWPPRA project and program effectiveness. The vegetation analytical teams plan to summarize their results in the form of written reports and/or graphics and present these items to CRMS Federal and State sponsors, restoration project managers, landowners, and other data users for their input.

Open-File Report↗

A revised logistic regression equation and an automated procedure for mapping the probability of a stream flowing perennially in Massachusetts

A revised logistic regression equation and an automated procedure were developed for mapping the probability of a stream flowing perennially in Massachusetts. The equation provides city and town conservation commissions and the Massachusetts Department of Environmental Protection a method for assessing whether streams are intermittent or perennial at a specific site in Massachusetts by estimating the probability of a stream flowing perennially at that site. This information could assist the environmental agencies who administer the Commonwealth of Massachusetts Rivers Protection Act of 1996, which establishes a 200-foot-wide protected riverfront area extending from the mean annual high-water line along each side of a perennial stream, with exceptions for some urban areas. The equation was developed by relating the observed intermittent or perennial status of a stream site to selected basin characteristics of naturally flowing streams (defined as having no regulation by dams, surface-water withdrawals, ground-water withdrawals, diversion, wastewater discharge, and so forth) in Massachusetts. This revised equation differs from the equation developed in a previous U.S. Geological Survey study in that it is solely based on visual observations of the intermittent or perennial status of stream sites across Massachusetts and on the evaluation of several additional basin and land-use characteristics as potential explanatory variables in the logistic regression analysis. The revised equation estimated more accurately the intermittent or perennial status of the observed stream sites than the equation from the previous study. Stream sites used in the analysis were identified as intermittent or perennial based on visual observation during low-flow periods from late July through early September 2001. The database of intermittent and perennial streams included a total of 351 naturally flowing (no regulation) sites, of which 85 were observed to be intermittent and 266 perennial. Stream sites included in the database had drainage areas that ranged from 0.04 to 10.96 square miles. Of the 66 stream sites with drainage areas greater than 2.00 square miles, 2 sites were intermittent and 64 sites were perennial. Thus, stream sites with drainage areas greater than 2.00 square miles were assumed to flow perennially, and the database used to develop the logistic regression equation included only those stream sites with drainage areas less than 2.00 square miles. The database for the equation included 285 stream sites that had drainage areas less than 2.00 square miles, of which 83 sites were intermittent and 202 sites were perennial. Results of the logistic regression analysis indicate that the probability of a stream flowing perennially at a specific site in Massachusetts can be estimated as a function of four explanatory variables: (1) drainage area (natural logarithm), (2) areal percentage of sand and gravel deposits, (3) areal percentage of forest land, and (4) region of the state (eastern region or western region). Although the equation provides an objective means of determining the probability of a stream flowing perennially at a specific site, the reliability of the equation is constrained by the data used in its development. The equation is not recommended for (1) losing stream reaches or (2) streams whose ground-water contributing areas do not coincide with their surface-water drainage areas, such as many streams draining the Southeast Coastal Region-the southern part of the South Coastal Basin, the eastern part of the Buzzards Bay Basin, and the entire area of the Cape Cod and the Islands Basins. If the equation were used on a regulated stream site, the estimated intermittent or perennial status would reflect the natural flow conditions for that site. An automated mapping procedure was developed to determine the intermittent or perennial status of stream sites along reaches throughout a basin. The procedure delineates the drainage area boundaries, determines values for the four explanatory variables, and solves the equation for estimating the probability of a stream flowing perennially at two locations on a headwater (first-order) stream reach-one near its confluence or end point and one near its headwaters or start point. The automated procedure then determines the intermittent or perennial status of the reach on the basis of the calculated probability values and a probability cutpoint (a stream is considered to flow perennially at a cutpoint of 0.56 or greater for this study) for the two locations or continues to loop upstream or downstream between locations less than and greater than the cutpoint of 0.56 to determine the transition point from an intermittent to a perennial stream. If the first-order stream reach is determined to be intermittent, the procedure moves to the next downstream reach and repeats the same process. The automated procedure then moves to the next first-order stream and repeats the process until the entire basin is mapped. A map of the intermittent and perennial stream reaches in the Shawsheen River Basin is provided on a CD-ROM that accompanies this report. The CD-ROM also contains ArcReader 9.0, a freeware product, that allows a user to zoom in and out, set a scale, pan, turn on and off map layers (such as a USGS topographic map), and print a map of the stream site with a scale bar. Maps of the intermittent and perennial stream reaches in Massachusetts will provide city and town conservation commissions and the Massachusetts Department of Environmental Protection with an additional method for assessing the intermittent or perennial status of stream sites.

Scientific Investigations Report↗

Urban stormwater: An overlooked pathway of extensive mixed contaminants to surface and groundwaters in the United States

Increasing global reliance on stormwater control measures to reduce discharge to surface water, increase groundwater recharge, and minimize contaminant delivery to receiving waterbodies necessitates improved understanding of stormwater-contaminant profiles. A multi-agency study of organic and inorganic chemicals in urban stormwater from 50 runoff events at 21 sites across the United States demonstrated that stormwater transports substantial mixtures of polycyclic aromatic hydrocarbons, bioactive contaminants (pesticides and pharmaceuticals), and other organic chemicals known or suspected to pose environmental health concern. Numerous organic-chemical detections per site (median number of chemicals detected = 73), individual concentrations exceeding 10,000 ng/L, and cumulative concentrations up to 263,000 ng/L suggested concern for potential environmental effects during runoff events. Organic concentrations, loads, and yields were positively correlated with impervious surfaces and highly developed urban catchments. Episodic storm-event organic concentrations and loads were comparable to and often exceeded those of daily wastewater plant discharges. Inorganic chemical concentrations were generally dilute in concentration and did not exceed chronic aquatic life criteria. Methylmercury was measured in 90% of samples with concentrations that ranged from 0.05 to 1.0 ng/L.

Environmental Science & Technology↗

Some observations on the ecology and fish management of reservoirs in the United States

By 1980 there were 1,608 reservoirs (over 202 ha each) with a surface area of 4 million ha in the United States. Construction of new reservoirs has decreased in recent years because of the lack of suitable sites and the opposition of environmental interests. Reservoirs have characteristics of both lacustrine and riverine ecosystems. Reservoirs have been segmented into riverine, transitional and lacustrine zones for simulation modelling.Fish and plankton production increases rapidly after initial impoundment and then decreases and stabilizes at lower levels. Decreases in the number of fish species and in fish biomass following impoundment are caused by reduced quantity and quality of spawning and nursery habitat and decreased detrital input. Plankton populations can be followed downstream in a series of reservoirs and increases in abundance were associated with the inundation of vegetation or the introduction of nutrients.In addition to water level management, the most effective fish management programs have included introducing new fishes, developing vegetation or building structures on the reservoir bottom. Considerable progress has been made in predicting the effects of environmental parameters on fish production.

Montana, Nebraska, North Dakota, South Dakota↗

Over 100 years of environmental change recorded by foraminifers and sediments in a large Gulf of Mexico estuary, Mobile Bay, AL, USA

The marine microfauna of Mobile Bay has been profoundly influenced by the development and expansion of the primary shipping channel over the last ∼100 years. Foraminifers and sediments from seven box cores with excess lead-210 chronology document that channel dredging and spoil disposal have altered circulation, reduced estuarine mixing, changed sedimentation patterns, and caused a faunal turnover within the bay. Beginning in the late 1800s, changes in estuarine mixing allowed for greater low-pH freshwater influence in the bay, and ultimately began environmental changes that resulted in the loss of calcareous foraminifers. By the early 1900s, box cores throughout Mobile Bay record a ∼100-year trend of increasing calcareous test dissolution that continues to the present. Since the completion of the current shipping channel in the 1950s, restricted tidal flushing and increased terrestrial organic matter, documented by carbon-to-nitrogen ratios, stimulated an increase in agglutinated foraminiferal densities. However, in deeper areas of the bay, hypoxic water has negatively impacted the marine microfauna. Comparisons of the present-day foraminiferal assemblage with foraminifers collected in the early 1970s indicate that the continued biologic loss of calcareous foraminifers in the bay has allowed the introduction of a new agglutinated foraminiferal species into the bay.

Alabama↗

Application of the Hydroecological Integrity Assessment Process for Missouri Streams

Natural flow regime concepts and theories have established the justification for maintaining or restoring the range of natural hydrologic variability so that physiochemical processes, native biodiversity, and the evolutionary potential of aquatic and riparian assemblages can be sustained. A synthesis of recent research advances in hydroecology, coupled with stream classification using hydroecologically relevant indices, has produced the Hydroecological Integrity Assessment Process (HIP). HIP consists of (1) a regional classification of streams into hydrologic stream types based on flow data from long-term gaging-station records for relatively unmodified streams, (2) an identification of stream-type specific indices that address 11 subcomponents of the flow regime, (3) an ability to establish environmental flow standards, (4) an evaluation of hydrologic alteration, and (5) a capacity to conduct alternative analyses. The process starts with the identification of a hydrologic baseline (reference condition) for selected locations, uses flow data from a stream-gage network, and proceeds to classify streams into hydrologic stream types. Concurrently, the analysis identifies a set of non-redundant and ecologically relevant hydrologic indices for 11 subcomponents of flow for each stream type. Furthermore, regional hydrologic models for synthesizing flow conditions across a region and the development of flow-ecology response relations for each stream type can be added to further enhance the process. The application of HIP to Missouri streams identified five stream types ((1) intermittent, (2) perennial runoff-flashy, (3) perennial runoff-moderate baseflow, (4) perennial groundwater-stable, and (5) perennial groundwater-super stable). Two Missouri-specific computer software programs were developed: (1) a Missouri Hydrologic Assessment Tool (MOHAT) which is used to establish a hydrologic baseline, provide options for setting environmental flow standards, and compare past and proposed hydrologic alterations; and (2) a Missouri Stream Classification Tool (MOSCT) designed for placing previously unclassified streams into one of the five pre-defined stream types.

Open-File Report↗

Widespread occupancy of the endangered northern myotis on northeastern Atlantic Coastal Plain islands

Northern myotis Myotis septentrionalis are one of the bat species most affected by white-nose syndrome (WNS), and disease-induced declines may cause compounding effects when combined with other threats such as habitat loss and fragmentation. Recent evidence suggests that peripheral populations are persisting in post-WNS years; however, the environmental factors that influence the occurrence of this species along the Atlantic Coastal Plain are virtually unknown. We conducted a large-scale acoustic survey on 3 islands: Long Island, New York, and Martha’s Vineyard and Nantucket, Massachusetts, USA, and used a multi-scale occupancy modeling approach to determine the landscape and abiotic factors affecting the distribution of northern myotis. Our estimates of occupancy and detection probability suggest widespread presence across the islands. At the local (200 m) scale, we identified strong negative effects of development on Long Island and Nantucket and a strong positive effect of forest habitat on Martha’s Vineyard. None of the variables we measured sufficiently explained the landscape (1 km 2 ) occupancy of this species, which was very high (ψ = 0.81–0.97), representing an outlier for this species in the post-WNS landscape. The lack of association at the landscape scale suggests that general differences in land cover are not a driving factor of higher occupancy of peripheral northern myotis populations, while local site- specific conditions may be supporting critical foraging or roosting habitat. Because islands are particularly vulnerable to human-driven habitat alteration due to the constraint of limited space, and development pressure is expected to increase, this study provides a baseline to enable managers to assess the effects of future environmental disturbances and monitor population trends to support long-term survival of northern myotis.

Massachusetts, New York↗

Estimating wildfire risk on a Mojave Desert landscape using remote sensing and field sampling

Predicting wildfires that affect broad landscapes is important for allocating suppression resources and guiding land management. Wildfire prediction in the south-western United States is of specific concern because of the increasing prevalence and severe effects of fire on desert shrublands and the current lack of accurate fire prediction tools. We developed a fire risk model to predict fire occurrence in a north-eastern Mojave Desert landscape. First we developed a spatial model using remote sensing data to predict fuel loads based on field estimates of fuels. We then modelled fire risk (interactions of fuel characteristics and environmental conditions conducive to wildfire) using satellite imagery, our model of fuel loads, and spatial data on ignition potential (lightning strikes and distance to roads), topography (elevation and aspect) and climate (maximum and minimum temperatures). The risk model was developed during a fire year at our study landscape and validated at a nearby landscape; model performance was accurate and similar at both sites. This study demonstrates that remote sensing techniques used in combination with field surveys can accurately predict wildfire risk in the Mojave Desert and may be applicable to other arid and semiarid lands where wildfires are prevalent.

Mojave Desert↗

Toxicity and accumulation of silver nanoparticles during development of the marine polychaete Platynereis dumerilii

Pollutants affecting species at the population level generate ecological instability in natural systems. The success of early life stages, such as those of aquatic invertebrates, is highly affected by adverse environmental conditions. Silver released into the environment from emerging nanotechnology represents such a threat. Sediments are sinks for numerous pollutants, which aggregate and/or associate with depositing suspended particles. Deposit feeder such as the annelid Platynereis dumerilii, which has a large associated literature on its development, is an excellent model organism for exposure studies in coastal environments. We exposed eggs, larvae, juveniles and adults of P. dumerilii to various concentrations of citrate (cit-Ag NPs) or humic acid (HA-Ag NPs) capped silver nanoparticles (Ag NPs) as well to dissolved Ag (added as AgNO3). We showed that mortality and abnormal development rate increased with younger life stages. While adults and juvenile were the most tolerant life stages, fertilized eggs were highly sensitive to AgNO3, cit-Ag NPs and HA-Ag NPs. Exposures to HA-Ag NPs triggered the highest cute toxicity responses in P. dumerilii and in most cases both Ag NPs were more toxic than AgNO3. Uptake rate of HA-Ag NPs in adult worms was also higher than from other Ag forms, consistent with toxicity to other life stages. The early stages of the life cycle of marine coastal organisms are more affected by Ag NPs than the juvenile or adult life stages, indicating that exposure experiments at the larval level contribute to realistic eco-toxicological studies in aquatic environments.

Science of the Total Environment↗

Assessment of population genetics and climatic variability can refine climate‐informed seed transfer guidelines

Restoration guidelines increasingly recognize the importance of genetic attributes in translocating native plant materials (NPMs). However, when species‐specific genetic information is unavailable, seed transfer guidelines use climate‐informed seed transfer zones (CSTZs) as an approximation. While CSTZs may improve how NPMs are developed and/or matched to restoration sites, they overlook genetic factors that can diminish restoration success and/or deteriorate natural patterns of genetic diversity and environmental factors that may introduce unexpected variation. Here, we analyze molecular data and geographic patterns of environmental variability across the western United States and demonstrate how they can refine CSTZs. Using genetic data available for 13 relevant plant species, we found that the probability of mixing genetically differentiated individuals (i.e. from different evolutionary lineages, or populations) was approximately 8% when considering locations separated by 50 km and reached nearly 80% by 500 km, which are distances relevant to ecoregionally constrained CSTZs. Furthermore, climate analyses revealed that geographically proximate locations are likely to maintain environmental similarity, regardless of CSTZ or ecoregion assignment. These results suggest constraining CSTZ‐informed seed transfer decisions by distance may mitigate the opportunity for negative genetic outcomes. Furthermore, environmental variability and/or specificity of NPMs (depending upon the restoration strategy) should be achieved by sourcing NPMs from geographically proximate locations to avoid introducing excessive genetic differentiation. Our results highlight the utility of combining molecular genetic data with other genetic inferences (i.e. of adaptation) to determine how best to transfer seed across restoration species' ranges and develop new restoration materials.

Restoration Ecology↗