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Comprehensive water quality of the Boulder Creek Watershed, Colorado, during high-flow and low-flow conditions, 2000

Executive Summary The Boulder Creek Watershed, Colorado, is 1160 square kilometers in area and ranges in elevation from 1480 to 4120 meters above sea level. Streamflow originates primarily as snowmelt near the Continental Divide, and thus discharge varies seasonally and annually (Chapter 1). Most of the water in Boulder Creek is diverted for domestic, agricultural, and industrial use. Some diverted water is returned to the creek as wastewater effluent and by ditch returns, and additional water enters as groundwater and by transbasin diversions. These diversions and returns lead to complex temporal and spatial variations in discharge. The variations in discharge, along with natural factors such as geology and climate, and anthropogenic factors such as wastewater treatment, agriculture, mining, and urbanization, can affect water chemistry. As with many watersheds in the American West, dependable water quality and sufficient water supply are issues facing local water managers and users. Detailed water-quality and sediment sampling allows the identification of sources and sinks of chemical constituents and an understanding of the processes at work in a river system. This study, the most comprehensive water-quality analysis performed for Boulder Creek to date, was a cooperative effort of the U.S. Geological Survey (USGS) and the city of Boulder. Geographic information systems and modeling programs were used to delineate watershed boundaries, land cover, and geology (Chapter 2). During high-flow (June 2000) and low-flow (October 2000) conditions, researchers evaluated 226 water-quality variables, including basic water-quality indicators (Chapter 3), major ions and trace elements (Chapter 4), wastewater-derived organic compounds (Chapter 5), and pesticides (Chapter 6). Discharge (Chapter 1) and bed-sediment particle size and mineralogy (Chapter 7) were also evaluated. This cooperative study was facilitated by the Boulder Area Sustainability Information Network (BASIN), which provides public access to environmental information about the Boulder Creek Watershed on a website, www.basin.org. In addition to the USGS and city of Boulder data, researchers at the Institute of Arctic and Alpine Research at the University of Colorado provided water chemistry data for the headwaters of North Boulder Creek, upstream of the reach of the USGS/city of Boulder sampling sites (Chapter 8). Snowmelt produces high flows in Boulder Creek in late spring to early summer (Chapter 1). Because precipitation falling in the headwaters is very dilute (specific conductance about 5 microsiemens per centimeter), most chemical constituents are present in lower concentrations during high flows (Chapters 3, 4, 5, 6, and 8). However, concentrations of some constituents, such as total suspended solids (Chapter 3) and organic carbon (Chapter 5), increase during the spring snowmelt flush. The upper basin, which consists of alpine, subalpine, montane, and foothills regions west of the mouth of Boulder Canyon, is underlain by Precambrian igneous and metamorphic rocks (Chapter 1). Major dissolved inorganic constituents in headwater sites were found to be enriched by factors of 10 to 20 relative to precipitation; this is consistent with minor weathering of the local crystalline bedrock (Chapter 4). Some anthropogenic input is observed in the headwaters; precipitation introduces nitrogen derived from fossil fuel combustion and agricultural activities (Chapter 8). The lower basin, which consists of the plains region east of the mouth of Boulder Canyon, is underlain by Mesozoic sedimentary rock and Quaternary alluvium, and has substantially more anthropogenic sources. Concentrations of most dissolved inorganic constituents increased in the lower basin. Differentiation between natural and anthropogenic sources of some dissolved constituents is difficult because both sources contribute to the water composition in this region. The increase of most major constituents (bicarbonate, calcium, chloride, magnesium, sodium, and sulfate) is consistent with weathering of the underlying sedimentary bedrock (Chapter 4). It is likely that anthropogenic loading of constituents in this reach occurs during storm events. Fecal coliform concentrations were variable and in some cases exceeded state standards, primarily during low-flow conditions (Chapter 3). Effluent from Boulder’s 75th Street Wastewater Treatment Plant (WWTP) has a substantial impact on the water chemistry of lower Boulder Creek. The WWTP increases the concentrations of nutrients such as nitrogen and phosphorus (Chapter 3), major ions and trace metals (Chapter 4), and organic carbon (Chapter 5) in Boulder Creek. The effluent contained a spike in gadolinium, a rare earth element that is ingested for magnetic resonance imaging as a contrasting agent and then excreted to the urban wastewater system. The effluent also contained trace organic compounds such as surfactants, pharmaceuticals, hormones (Chapter 5), and pesticides (Chapter 6), which also were detected at downstream Boulder Creek sites. Water chemistry of Boulder Creek downstream of the WWTP is largely controlled by the degree of dilution of the wastewater effluent, which varies depending on the baseflow of Boulder Creek, the volume of wastewater effluent, and depletion by agricultural diversions. Coal Creek, a tributary of Boulder Creek, contains wastewater effluent from four additional WWTPs, and increases the load of many constituents in Boulder Creek. In addition to the impact from wastewater effluent, lower Boulder Creek is affected by agricultural land use. Eleven of 84 analyzed pesticides were detected in Boulder Creek or its inflows, primarily in the eastern section of the watershed (Chapter 6). This collaborative study provides an in-depth evaluation of the hydrology, water chemistry, and sediment mineralogy of North Boulder Creek, Middle Boulder Creek, Boulder Creek, and major inflows. The detailed sampling and analysis in this report provide a baseline for future reference, as well as information on the effect of land use and geology on water chemistry.

Colorado↗

Effects of stream flow intermittency on riparian vegetation of a semiarid region river (San Pedro River, Arizona)

The San Pedro River in the southwestern United States retains a natural flood regime and has several reaches with perennial stream flow and shallow ground water. However, much of the river flows intermittently. Urbanization-linked declines in regional ground-water levels have raised concerns over the future status of the riverine ecosystem in some parts of the river, while restoration-linked decreases in agricultural ground-water pumping are expected to increase stream flows in other parts. This study describes the response of the streamside herbaceous vegetation to changes in stream flow permanence. During the early summer dry season, streamside herbaceous cover and species richness declined continuously across spatial gradients of flow permanence, and composition shifted from hydric to mesic species at sites with more intermittent flow. Hydrologic threshold values were evident for one plant functional group: Schoenoplectus acutus, Juncus torreyi, and other hydric riparian plants declined sharply in cover with loss of perennial stream flow. In contrast, cover of mesic riparian perennials (including Cynodon dactylon, an introduced species) increased at sites with intermittent flow. Patterns of hydric and mesic riparian annuals varied by season: in the early summer dry season their cover declined continuously as flow became more intermittent, while in the late summer wet season their cover increased as the flow became more intermittent. Periodic drought at the intermittent sites may increase opportunities for establishment of these annuals during the monsoonal flood season. During the late summer flood season, stream flow was present at most sites, and fewer vegetation traits were correlated with flow permanence; cover and richness were correlated with other environmental factors including site elevation and substrate nitrate level and particle size. Although perennial-flow and intermittent-flow sites support different streamside plant communities, all of the plant functional groups are abundant at perennial-flow sites when viewing the ecosystem at broader spatial and temporal scales: mesic riparian perennials are common in the floodplain zone adjacent to the river channel and late-summer hydric and mesic annuals are periodically abundant after large floods. Copyright ?? 2005 John Wiley & Sons, Ltd.

River Research and Applications↗

Reconnaissance of cumulative risk of pesticides and pharmaceuticals in Great Smoky Mountains National Park streams

The United States (US) National Park Service (NPS) manages protected public lands to preserve biodiversity. Exposure to and effects of bioactive organic contaminants in NPS streams are challenges for resource managers. Recent assessment of pesticides and pharmaceuticals in protected-streams within the urbanized NPS Southeast Region (SER) indicated the importance of fluvial inflows from external sources as drivers of aquatic contaminant-mixture exposures. Great Smoky Mountains National Park (GRSM), lies within SER, has the highest biodiversity and annual visitation of NPS parks, but, in contrast to the previously studied systems, straddles a high-elevation hydrologic divide; this setting limits fluvial-inflows of contaminants but potentially increases visitation-driven contaminant deliveries. We leveraged the unique characteristics of GRSM to test further the importance of fluvial contaminant inflows as drivers of protected-stream exposures and to inform the relative importance of potential additional contaminant transport mechanisms, by comparing the estimated risks of 328 pesticides and pharmaceuticals in water at 16 GRSM stream locations to those estimated previously in SER streams. Extensive mixtures (31 compounds) were only observed in an atypical reach on the boundary of GRSM downstream of a wastewater discharge, while limited mixtures (2–5 compounds) were observed in one stream with elevated visitation pressure (recreational “tube floating”). The insecticide, imidacloprid, used to eradicate hemlock woolly adelgid, was detected in 8 (50%) streams. Infrequent exceedances of a cumulative ToxCast-based, exposure-activity ratio (Σ EAR ) 0.001 screening-level of concern suggested limited risk to non-target, aquatic vertebrates, whereas exceedances of a cumulative benchmark-based, invertebrate toxicity quotient (Σ TQ ) 0.1 screening level at 8 locations indicated generally high risk to invertebrates. The results are consistent with the importance of fluvial transport from extra-park sources as a driver of bioactive-contaminant mixture exposures in protected streams and illustrate the potential additional risks from visitation-driven and tactical-use-pesticides.

Tennessee↗

Connecting ground water influxes with fish species diversity in an urbanized watershed

Valley Creek watershed is a small stream system that feeds the Schuylkill River near Philadelphia, Pennsylvania. The watershed is highly urbanized, including over 17 percent impervious surface cover (ISC) by area. Imperviousness in a watershed has been linked to fish community structure and integrity. Generally, above 10 to 12 percent ISC there is marked decline in fish assemblages with fish being absent above 25 percent ISC. This study quantifies the importance of ground water in maintaining fish species diversity in subbasins with over 30 percent ISC. Valley Creek contains an atypical fish assemblage in that the majority of the fish are warm-water species, and the stream supports naturally reproducing brown trout, which were introduced and stocked from the early 1900s to 1985. Fish communities were quantified at 13 stations throughout the watershed, and Simpson's species diversity index was calculated. One hundred and nine springs were located, and their flow rates measured. A cross covariance analysis between Simpson's species diversity index and spring flow rates upstream of fish stations was performed to quantify the spatial correlation between these two variables. The correlation was found to be highest at lag distances up to about 400 m and drop off significantly beyond lag distances of about 800 m.

Pennsylvania↗

Collection and analysis of samples for polycyclic aromatic hydrocarbons in dust and other solids related to sealed and unsealed pavement from 10 cities across the United States, 2005-07

Parking lots and driveways are dominant features of the modern urban landscape, and in the United States, sealcoat is widely used on these surfaces. One of the most widely used types of sealcoat contains refined coal tar; coal-tar-based sealcoat products have a mean polycyclic aromatic hydrocarbon (PAH) concentration of about 5 percent. A previous study reported that parking lots in Austin, Texas, treated with coal-tar sealcoat were a major source of PAH compounds in streams. This report presents methods for and data from the analysis of concentrations of PAH compounds in dust from sealed and unsealed pavement from nine U.S. cities, and concentrations of PAH compounds in other related solid materials (sealcoat surface scrapings, nearby street dust, and nearby soil) from three of those same cities and a 10th city. Dust samples were collected by sweeping dust from areas of several square meters with a soft nylon brush into a dustpan. Some samples were from individual lots or driveways, and some samples consisted of approximately equal amounts of material from three lots. Samples were sieved to remove coarse sand and gravel and analyzed by gas chromatography/mass spectrometry. Concentrations of PAHs vary greatly among samples with total PAH (sigmaPAH), the sum of 12 unsubstituted parent PAHs, ranging from nondetection for all 12 PAHs (several samples from Portland, Oregon, and Seattle, Washington; sigmaPAH of less than 36,000 micrograms per kilogram) to 19,000,000 micrograms per kilogram for a sealcoat scraping sample (Milwaukee, Wisconsin). The largest PAH concentrations in dust are from a driveway sample from suburban Chicago, Illinois (sigmaPAH of 9,600,000 micrograms per kilogram).

Data Series↗

Water-Quality Assessment of Southern Florida - Wastewater Discharges and Runoff

Nearly 800 million gallons per day of treated wastewater was discharged in the Southern Florida National Water-Quality Assessment (NAWQA) study unit in 1990, most to the Atlantic Ocean (44 percent) and to deep, saline aquifers (25 percent). About 9 percent was discharged to fresh surface waters and about 22 percent to shallow ground water, of which septic tanks accounted for 9 percent. Runoff from agricultural and urban lands, though not directly measured, is a large source of wastewater in southern Florida.

Fact Sheet↗

Pesticides in the Lower Clackamas River Basin, Oregon, 2000-01

In 2000-01, the U. S. Geological Survey sampled the Clackamas River and its major lower-basin tributaries during storm runoff conditions for 86 dissolved pesticides and selected breakdown products. Twenty-seven compounds, including 18 herbicides, 7 insecticides, and 2 pesticide breakdown products, were detected in 18 stream samples. The most commonly detected pesticides, in decreasing frequency, included atrazine, simazine, diazinon, metolachlor, and diuron, which variously occurred in 46-92% of samples collected from the tributaries. Of these, atrazine, simazine, and metolachlor, plus six other compounds, also were detected in the main-stem Clackamas River. Pesticides were detected more frequently and at higher concentrations in the four lowermost tributaries (Deep, Richardson, Rock, and Sieben Creeks). In these streams, 12 to 18 pesticides were detected per stream in samples collected during spring and fall. Pesticides always occurred with at least one other pesticide, and about half of the samples, including one sample from the Clackamas River in October 2000, contained six or more pesticides. Nine pesticides, including the insecticide diazinon and the herbicides 2,4-D, atrazine, dichlobenil, diuron, imazaquin, metolachlor, simazine, and trifluralin, were detected in five water samples of Clackamas River water. No pesticides were detected in three samples of treated Clackamas River water used for drinking-water supply. Concentrations of six compounds--carbaryl, chlorpyrifos, diazinon, dieldrin, malathion, and the breakdown product of DDT (p,p'-DDE)--exceeded established or recommended criteria for the protection of aquatic life in some of the tributaries, sometimes for multiple pesticides in one sample. Identifying the sources of pesticides detected in the Clackamas River Basin is difficult because of the diverse land use in the basin and the multiple-use nature of many of the pesticides detected. Of the 25 parent compounds detected, 22 have agricultural uses, 23 have urban uses, 16 are applied to golf courses, 11 are applied along roads and other right-of-ways, and 5 have or had forestry applications. Because only a small fraction of the thousands of pesticide products registered for use in Oregon were tested for in this study, future monitoring could benefit from knowledge of what pesticides are applied so that potential problems can be identified and managed.

Water-Resources Investigations Report↗

Application of MODFLOW’s farm process to California’s Central Valley

Historically, California’s Central Valley has been one of the most productive agricultural regions in the world. The Central Valley also is rapidly becoming an important area for California’s expanding urban population. During 1980–2007, the population nearly doubled in the Central Valley, increasing the competition for water. Because of the importance of ground water in the Central Valley, the U.S. Geological Survey (USGS) Ground-Water Resources Program is evaluating ground-water conditions in the valley on the basis of historical and anticipated water use. This study updates the USGS Central Valley Regional Aquifer System and Analysis (CVRASA) model that was originally? calibrated to observed conditions for the period 1961-77. The model developed for this study utilizes MODFLOW-2000, and was calibrated to observed conditions for the period 1961-2003. Key updates include characterization of the aquifer system using a detailed textural analysis of more than 8,500 drillers’ logs; use of the MODFLOW subsidence package (SUB) to simulate aquifer-system compaction; and, most importantly, use of the newly developed MODFLOW Farm Process (FMP) for simulating irrigation and other landscape processes. The FMP provides coupled simulation of the ground-water and surface-water components of the hydrologic cycle for irrigated and non-irrigated areas. A dynamic allocation of ground-water recharge and ground-water pumping is simulated on the basis of residual crop-water demand after surface-water deliveries and root uptake from shallow ground water. The FMP links with the Streamflow Routing Package SFR1) to facilitate the simulated conveyance of surface-water deliveries. Ground-water Pumpage through both single-aquifer and multi-node wells, irrigation return flow, and variable irrigation efficiencies also are simulated by the FMP. The simulated deliveries and ground-water pumpage in the updated model reflect climatic differences, differences among defined water-balance regions, and changes in the waterdelivery system, during the 1961–2003 simulation period. The model is designed to accept forecasts from Global Climate Models (GCMs) to simulate the potential effects on surface-water delivery, ground-water pumpage, and ground-water storage in response to climate change. The model provides a detailed transient analysis of changes in ground-water availability in relation to climatic variability, urbanization, and changes in irrigated agriculture.

Conference Paper↗

Effect of urbanization on the water resources of Warminster Township, Bucks County, Pennsylvania

Rapid suburban development occurred in Warminster Township and the surrounding area after World War II, resulting in a large population dependent on ground water. In 1980, approximately 2.7 billion gallons of ground water was pumped by public water suppliers and government facilities. Pumping wells can cause drawdown as far as 2,500 feet undip, downdip, or along strike even if the wells do not penetrate the same strata. Pumping wells have lowered base flow; a stream-gain-and-loss study showed that water lost from Little Neshaminy Creek was about 60 percent of the water pumped from wells near the stream. Net ground-water infiltration to sewers was about 830 million gallons in 1979, a wet year, and about 250 million gallons in 1980, a dry year. Estimated water budgets for 1979 and 1980 indicate evapotranspiration can range from 20 to 26 inches per year (1.0 to 1.2 million gallons per day per square mile) and recharge can range from 8 to 18 inches per year (0.4 to 0.9 million gallons per day per square mile). In a year with average precipitation (45 inches or 2.1 million gallons per day per square mile), evapotranspiration is about 24 inches (1.1 million gallons per day per square mile). Ground-water development in the area influenced by pumping is at its practical limit for years of average recharge, but as much as 1.1 million gallons per day of additional water may be obtained by drilling and pumping wells in areas of Warminster Township not affected by pumping. The concentration of most dissolved constituents increased in water from seven wells, sampled at the onset of urbanization in 1953 and 1956 and again in 1979. Ground-water contamination by volatile organic compounds, especially trichloroethylene and tetrachloroethylene, has made water from some wells unsuitable for public supply. The concentration of lead in 26 samples of ground water ranged from 0 to 55 micrograms per liter, with a median of 17 micrograms per liter; this is above the reported national median and the median in nearby Chester County. High concentrations of sulfate and dissolved solids in ground water are probably caused by restricted gournd-water circulation and may be reduced by long-term pumping, which flushes the aquifer. Effluent from sewage treatment plants has degraded the quality of low streamflow.

Pennsylvania↗

Watershed characteristics and pre-restoration surface-water hydrology of Minebank Run, Baltimore County, Maryland, water years 2002-04

Stream restoration efforts have been ongoing in Maryland since the early 1990s. Physical stream restoration often involves replacement of lost sediments to elevate degraded streambeds, re-establishment of riffle-pool sequences along the channel profile, planting vegetation in riparian zones, and re-constructing channel banks, point bars, flood plains, and stream-meanders. The primary goal of many restoration efforts is to re-establish geomorphic stability of the stream channel and reduce erosive energy from urban runoff. Monitoring streams prior to and after restoration could help quantify other possible benefits of stream restoration, such as improved water quality and biota. This report presents general watershed characteristics associated with the Minebank Run watershed; a small, urban watershed in the south-central section of Baltimore County, Maryland that was physically restored in phases during 1999, 2004, and 2005. The physiography, geology, hydrology, land use, soils, and pre-restoration geomorphic setting of the unrestored stream channel are discussed. The report describes a reach of Minebank Run that was selected for the purpose of collecting several types of environmental data prior to restoration, including continuous-record and partial-record stage and streamflow data, precipitation, and ground-water levels. Examples of surface-water data that were collected in and near the study reach during water years 2002 through 2004, including continuous-record streamflow, partial-record stage and discharge, and precipitation, are described. These data were used in analyses of several characteristics of surface-water hydrology in the watershed, including (1) rainfall totals, storm duration, and intensity, (2) instantaneous peak discharge and daily mean discharge, (3) stage-discharge ratings, (4) hydraulic-geometry relations, (5) water-surface slope, (6) time of concentration, (7) flood frequency, (8) flood volume, and (9) rainfall-runoff relations. Several hydrologic characteristics that are typical of urban environments were quantified by these analyses. These include (1) large ratios of peak discharge to daily mean discharge as an indicator of flashiness, (2) consistent shifting of the stage-discharge rating over short periods of time that indicates instability of the stream channel, (3) analyses of hydraulic-geometry relations that indicate mean velocities of 11 feet per second or more while the flow is contained in the stream channel, (4) discharges that are 4 to 5 times larger in Minebank Run for corresponding flood frequency recurrence intervals than in Slade Run, which is a Piedmont watershed of similar size with smaller percentages of urban development, and (5) flood waves that can travel through the stream channel at a velocity of 412 feet per minute, or 6.9 feet per second.

Maryland↗

Spatial and temporal variability of perfluoroalkyl and polyfluoroalkyl substances in major rivers of New Mexico, USA

Perfluoroalkyl and polyfluoroalkyl substances (PFAS) are ubiquitous in the environment, but sources that contribute to temporal and spatial variability in surface waters are not well defined. Many states are assessing PFAS in water resources, and insight from these statewide assessments can help guide future sampling efforts. A statewide assessment of 28 PFAS was conducted in New Mexico starting in 2020, and subsequent follow-up sampling has improved understanding of PFAS occurrence and sources throughout the state. PFAS were present in all major rivers of New Mexico (Rio Grande, Pecos River, San Juan River, Animas River, Canadian River, Gila River, Rio Chama, and Rio Puerco) with 13 of 28 analyzed PFAS (PFBA, PFPeA, PFHxA, PFHpA, PFOA, PFNA, PFDA, PFBs, PFPeS, PFHxS, PFOS, PFOSA, 6:2 FTS) detected from at least one sample for samples collected between 2020 and 2024. This study found high temporal and spatial variability—PFAS concentrations ranged from below the laboratory detection level to 156 ng/L, with concentrations generally increasing downstream on the major rivers. PFBS was the most frequently detected and highest concentration PFAS in this study, ranging from 1 to 93 ng/L, followed by PFBA and PFPeA, ranging from 0.9 to 32 ng/L. The average of the sum of PFAS detected increased by an order of magnitude from 4 to 46 ng/L in the Rio Grande as it flows through Albuquerque, the largest urban area in New Mexico. PFAS concentration increased by 58% after a stormflow pulse flushed over Albuquerque and contributed water to the Rio Grande. The contribution of wastewater to surface water resources varied diurnally as well as seasonally. Sampling multiple locations on major rivers across multiple seasons, taking into account known anthropogenic inputs, would enhance characterization of temporal and spatial variability of PFAS concentrations. Increased sampling frequency at sites with wastewater contribution and focused investigations in areas with higher than expected PFAS could increase understanding of potential sources and variability of source contributions.

New Mexico↗

Nationwide reconnaissance of contaminants of emerging concern in source and treated drinking waters of the United States: Pharmaceuticals

Mobile and persistent chemicals that are present in urban wastewater, such as pharmaceuticals, may survive on-site ormunicipal wastewater treatment and post-discharge environmental processes. These pharmaceuticals have the potential to reach surface and groundwaters, essential drinking-water sources. A joint, two-phase U.S. Geological Survey-U.S. Environmental Protection Agency study examined source and treated waters from 25 drinking-water treatment plants from across the United States. Treatment plants that had probable wastewater inputs to their source waters were selected to assess the prevalence of pharmaceuticals in such source waters, and to identify which pharmaceuticals persist through drinking-water treatment. All samples were analyzed for 24 pharmaceuticals in Phase I and for 118 in Phase II. In Phase I, 11 pharmaceuticals were detected in all source-water samples, with amaximumof nine pharmaceuticals detected in any one sample. The median number of pharmaceuticals for all 25 samples was five. Quantifiable pharmaceutical detections were fewer, with a maximum of five pharmaceuticals in any one sample and a median for all samples of two. In Phase II, 47 different pharmaceuticals were detected in all source-water samples, with a maximum of 41 pharmaceuticals detected in any one sample. The median number of pharmaceuticals for all 25 samples was eight. For 37 quantifiable pharmaceuticals in Phase II, median concentrations in source water were below 113 ng/L.

Science of the Total Environment↗

Regional patterns of pesticide concentrations in surface waters of New York in 1997

The predominant mixtures of pesticides found in New York surface waters consist of five principal components. First, herbicides commonly used on corn (atrazine, metolachlor, alachlor, cyanazine) and a herbicide degradate (deethylatrazine) were positively correlated to a corn-herbicide component, and watersheds with the highest corn-herbicide component scores were those in which large amounts of row crops are grown. Second, two insecticides (diazinon and carbaryl) and one herbicide (prometon) widely used in urban and residential settings were positively correlated to an urban/residential component. Watersheds with the highest urban/residential component scores were those with large amounts of urban and residential land use. A third component was related to two herbicides (EPTC and cyanazine) used on dry beans and corn, the fourth to an herbicide (simazine) and an insecticide (carbaryl) commonly used in orchards and vineyards, and the fifth to an herbicide (DCPA). Results of this study indicate that this approach can be used to: (1) identify common mixtures of pesticides in surface waters, (2) relate these mixtures to land use and pesticide applications, and (3) indicate regions where these mixtures of pesticides are commonly found.

New York↗

Relative abundance of coyotes (Canis latrans) influences gray fox (Urocyon cinereoargenteus) occupancy across the eastern United States

Gray fox ( Urocyon cinereoargenteus (Schreber, 1775)) populations in portions of the eastern United States have experienced declines whose trajectories differ from those of other mesocarnivore populations. One hypothesis is that gray fox declines may result from interspecific interactions, particularly competition with abundant coyotes ( Canis latrans Say, 1823). Alternatively, gray foxes may respond negatively to increased urbanization and reduced forest cover. To evaluate these hypotheses, we used single-species occupancy models of camera trap data to test the effects of habitat covariates, such as the amount of urbanization and forest, on coyote and gray fox occupancy. Additionally, we test the effect of an index based on an N -mixture model of the number of coyotes at each camera trap site on gray fox occupancy. Results indicate that occupancy probabilities of coyote and gray fox relate positively to the amount of forest, but they provided no evidence urban cover impacts gray foxes. Additionally, gray fox occupancy was negatively related to the index of the number of coyotes at each site. Our models support the idea that interactions with coyotes impact gray fox occupancy across the eastern United States. These results illustrate how large-scale studies can relate mechanisms identified within specific landscapes to phenomena observed at larger scales.

Canadian Journal of Zoology↗

Flood plains of the South Branch Shiawassee River, Livingston County, Michigan

This report presents the results of a flood-plain study of approximately 1.9 mi (3.1 km) of the South Branch Shiawassee River in Livingston County. This reach of stream s in a currently unincorporated area about 40 mi (64 km) northwest of Detroit. Although little development has taken place, the potential for development is great due to urban spread from the Detroit Metropolitan area. To implement local flood-plain management plans, areas subject to flooding must be defined. This report is intended to provide that information. The report has been prepared in cooperation with the Livingston County Planning Commission and the Michigan Department of Natural Resources The reach of river mapped extends from the south boundary of section 3, T.2 N., R.4 E., to the west bund land f Interstate Highway 96. Six maps at a scale of 1:2400 (1 in – 200 ft or 1 cm = 24 m) were prepared; the areas covered by each map are shown on figure 1. The flood-plain maps show the area that would be inundated by a flood that has an average recurrence interval of once in 100 years; for brevity such a flood is termed the “100-year flood”.

Michigan↗

Conifer health classification for Colorado, 2008

Colorado has undergone substantial changes in forests due to urbanization, wildfires, insect-caused tree mortality, and other human and environmental factors. The U.S. Geological Survey Rocky Mountain Geographic Science Center evaluated and developed a methodology for applying remotely-sensed imagery for assessing conifer health in Colorado. Two classes were identified for the purposes of this study: healthy and unhealthy (for example, an area the size of a 30- x 30-m pixel with 20 percent or greater visibly dead trees was defined as ?unhealthy?). Medium-resolution Landsat 5 Thematic Mapper imagery were collected. The normalized, reflectance-converted, cloud-filled Landsat scenes were merged to form a statewide image mosaic, and a Normalized Difference Vegetation Index (NDVI) and Renormalized Difference Infrared Index (RDII) were derived. A supervised maximum likelihood classification was done using the Landsat multispectral bands, the NDVI, the RDII, and 30-m U.S. Geological Survey National Elevation Dataset (NED). The classification was constrained to pixels identified in the updated landcover dataset as coniferous or mixed coniferous/deciduous vegetation. The statewide results were merged with a separate health assessment of Grand County, Colo., produced in late 2008. Sampling and validation was done by collecting field data and high-resolution imagery. The 86 percent overall classification accuracy attained in this study suggests that the data and methods used successfully characterized conifer conditions within Colorado. Although forest conditions for Lodgepole Pine (Pinus contorta) are easily characterized, classification uncertainty exists between healthy/unhealthy Ponderosa Pine (Pinus ponderosa), Pi?on (Pinus edulis), and Juniper (Juniperus sp.) vegetation. Some underestimation of conifer mortality in Summit County is likely, where recent (2008) cloud-free imagery was unavailable. These classification uncertainties are primarily due to the spatial and temporal resolution of Landsat, and of the NLCD derived from this sensor. It is believed that high- to moderate-resolution multispectral imagery, coupled with field data, could significantly reduce the uncertainty rates. The USGS produced a four-county follow-up conifer health assessment using high-resolution RapidEye remotely sensed imagery and field data collected in 2009.

Scientific Investigations Map↗

Simulations of Groundwater Flow and Particle Tracking Analysis in the Area Contributing Recharge to a Public-Supply Well near Tampa, Florida, 2002-05

Shallow ground water in the north-central Tampa Bay region, Florida, is affected by elevated nitrate concentrations, the presence of volatile organic compounds, and pesticides as a result of groundwater development and intensive urban land use. The region relies primarily on groundwater for drinking-water supplies. Sustainability of groundwater quality for public supply requires monitoring and understanding of the mechanisms controlling the vulnerability of public-supply wells to contamination. A single public-supply well was selected for intensive study based on the need to evaluate the dominant processes affecting the vulnerability of public-supply wells in the Upper Floridan aquifer in the City of Temple Terrace near Tampa, Florida, and the presence of a variety of chemical constituents in water from the well. A network of 29 monitoring wells was installed, and water and sediment samples were collected within the area contributing recharge to the selected public-supply well to support a detailed analysis of physical and chemical conditions and processes affecting the water chemistry in the well. A three-dimensional, steady-state groundwater flow model was developed to evaluate the age of groundwater reaching the well and to test hypotheses on the vulnerability of the well to nonpoint source input of nitrate. Particle tracking data were used to calculate environmental tracer concentrations of tritium and sulfur hexafluoride and to calibrate traveltimes and compute flow paths and advective travel times in the model area. The traveltime of particles reaching the selected public-supply well ranged from less than 1 day to 127.0 years, with a median of 13.1 years; nearly 45 percent of the simulated particle ages were less than about 10 years. Nitrate concentrations, derived primarily from residential/commercial fertilizer use and atmospheric deposition, were highest (2.4 and 6.11 milligrams per liter as nitrogen, median and maximum, respectively) in shallow groundwater from the surficial aquifer system and lowest (less than the detection level of 0.06 milligram per liter) in the deeper Upper Floridan aquifer. Denitrification occurred near the interface of the surficial aquifer system and the underlying intermediate confining unit, within the intermediate confining unit, and within the Upper Floridan aquifer because of reducing conditions in this part of the flow system. However, simulations indicate that the rapid movement of water from the surficial aquifer system to the selected public-supply well through karst features (sinkholes) and conduit layers that bypass the denitrifying zones (short-circuits), coupled with high pumping rates, allow nitrate to reach the selected public-supply well in concentrations that resemble those of the overlying surficial aquifer system. Water from the surficial aquifer system with elevated concentrations of nitrate and low concentrations of some volatile organic compounds and pesticides is expected to continue moving into the selected public-supply well, because calculated flux-weighted concentrations indicate the proportion of young affected water contributing to the well is likely to remain relatively stable over time. The calculated nitrate concentration in the selected public-supply well indicates a lag of 1 to 10 years between peak concentrations of nonpoint source contaminants in recharge and appearance in the well.

Scientific Investigations Report↗

Bacterial community diversity and potential eco-physiological roles in toxigenic blooms composed of Microcystis, Aphanizomenon or Planktothrix

Cyanobacterial toxicity, cyanotoxins, and their impact on aquatic ecosystems and human health are well documented. In comparison, less is known about bloom-associated bacterial communities. Co-occurring bacteria can influence bloom development, physiology and collapse, and may also provide a niche for pathogenic bacteria. Existing research focuses on the cyanosphere of Microcystis -dominated blooms, despite the increasing prevalence of filamentous genera ( Aphanizomenon and Planktothrix ). This pilot study aimed to broaden our understanding of the bacterial consortia attached to morphologically distinct cyanobacteria (coccoid and filamentous) dominating phytoplankton communities and to explore their potential roles in amplifying the impacts of cyanobacterial blooms. We investigated four shallow freshwater bodies across three continents and two climate zones: an urban pond in the USA, a dammed reservoir and a natural lake in Poland, and an urban water body in Singapore. Amplicon sequencing (16S rRNA gene) was used to characterize bacterial communities, while shotgun metagenomics identified nitrogen- and phosphorus-cycling genes to infer potential eco-physiological functions. Cyanobacteria dominated bacterioplankton assemblages at all sites (>35.6%), with bloom composition influencing toxigenic profiles. A mixed bloom of Microcystis , Snowella , and Aphanizomenon had the broadest range of cyanotoxin synthetase genes ( mcy E, cyr J, ana F and sxt A). Microcystis blooms correlated with increased Roseomonas , while Planktothrix co-occurred with Flavobacterium – both bacteria likely contribute to nutrient-cycling within blooms and represent potential opportunistic pathogens for aquatic organisms and humans. The Microcystis cyanosphere exhibited the highest number of significant positive correlations with bacteria (19 relations), compared to Planktothrix and Aphanizomenon (11 and 2 relations, respectively). Non-diazotrophic blooms of Microcystis and Planktothrix showed greater abundances of nitrogen – ( ure B, gln A, nar B, and nar HZ) and phosphorus-cycling genes ( pho BHPR and ppk 1), indicating a strong dependence on associated bacteria for nutrient acquisition compared to diazotrophic Aphanizomenon . These findings suggest that Aphanizomenon -dominated blooms may be sustained by simpler microbiomes. Our results provide preliminary evidence of cyanosphere heterogeneity potentially shaped by the dominance or coexistence of three morphologically and eco-physiologically distinct genera of cyanobacteria. A comprehensive knowledge of the taxonomy and functional roles of bloom-associated microbiomes is therefore essential to understand bloom activity, evaluate the environmental threat, and develop effective strategies for prevention and mitigation.

Frontiers in Microbiology↗