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USGS2009

Differential virulence mechanisms of infectious hematopoietic necrosis virus in rainbow trout (Oncorhynchus mykiss) include host entry and virus replication kinetics

Host specificity is a phenomenon exhibited by all viruses. For the fish rhabdovirus infectious hematopoietic necrosis virus (IHNV), differential specificity of virus strains from the U and M genogroups has been established both in the field and in experimental challenges. In rainbow trout (Oncorhynchus mykiss), M IHNV strains are consistently more prevalent and more virulent than U IHNV. The basis of the differential ability of these two IHNV genogroups to cause disease in rainbow trout was investigated in live infection challenges with representative U and M IHNV strains. When IHNV was delivered by intraperitoneal injection, the mortality caused by U IHNV increased, indicating that the low virulence of U IHNV is partly due to inefficiency in entering the trout host. Analyses of in vivo replication showed that U IHNV consistently had lower prevalence and lower viral load than M IHNV during the course of infection. In analyses of the host immune response, M IHNV-infected fish consistently had higher and longer expression of innate immune-related genes such as Mx-1. This suggests that the higher virulence of M IHNV is not due to suppression of the immune response in rainbow trout. Taken together, the results support a kinetics hypothesis wherein faster replication enables M IHNV to rapidly achieve a threshold level of virus necessary to override the strong host innate immune response. ?? 2009 SGM.

Journal of General Virology↗

USGS2013

Factors influencing detection of eDNA from a stream-dwelling amphibian

Environmental DNA (eDNA) methods for detecting and estimating abundance of aquatic species are emerging rapidly, but little is known about how processes such as secretion rate, environmental degradation, and time since colonization or extirpation from a given site affect eDNA measurements. Using stream-dwelling salamanders and quantitative PCR (qPCR) analysis, we conducted three experiments to assess eDNA: (i) production rate; (ii) persistence time under different temperature and light conditions; and (iii) detectability and concentration through time following experimental introduction and removal of salamanders into previously unoccupied streams. We found that 44–50 g individuals held in aquaria produced 77 ng eDNA/h for 2 h, after which production either slowed considerably or began to equilibrate with degradation. eDNA in both full-sun and shaded treatments degraded exponentially to <1% of the original concentration after 3 days. eDNA was no longer detectable in full-sun samples after 8 days, whereas eDNA was detected in 20% of shaded samples after 11 days and 100% of refrigerated control samples after 18 days. When translocated into unoccupied streams, salamanders were detectable after 6 h, but only when densities were relatively high (0.2481 individuals/m 2 ) and when samples were collected within 5 m of the animals. Concentrations of eDNA detected were very low and increased steadily from 6–24 h after introduction, reaching 0.0022 ng/L. Within 1 h of removing salamanders from the stream, eDNA was no longer detectable. These results suggest that eDNA detectability and concentration depend on production rates of individuals, environmental conditions, density of animals, and their residence time.

Molecular Ecology Resources↗

USGS2009

Effect of dietary α-tocopherol + ascorbic acid, selenium, and iron on oxidative stress in sub-yearling Chinook salmon ( Oncorhynchus tshawytscha Walbaum)

A three-variable central composite design coupled with surface-response analysis was used to examine the effects of dietary ??-tocopherol + ascorbic acid (TOCAA), selenium (Se), and iron (Fe) on indices of oxidative stress in juvenile spring Chinook salmon. Each dietary factor was tested at five levels for a total of fifteen dietary combinations (diets). Oxidative damage in liver and kidney (lipid peroxidation, protein carbonyls) and erythrocytes (erythrocyte resistance to peroxidative lysis, ERPL) was determined after feeding experimental diets for 16 (early December) and 28 (early March) weeks. Only TOCAA influenced oxidative stress in this study, with most measures of oxidative damage decreasing (liver lipid peroxidation in December and March; ERPL in December; liver protein carbonyl in March) with increasing levels of TOCAA. We also observed a TOCAA-stimulated increase in susceptibility of erythrocytes to peroxidative lysis in March at the highest levels of TOCAA. The data suggest that under most circumstances a progressive decrease in oxidative stress occurs as dietary TOCAA increases, but higher TOCAA concentrations can stimulate oxidative damage in some situations. Higher levels of TOCAA in the diet were required in March than in December to achieve comparable levels of protection against oxidative damage, which may have been due to physiological changes associated with the parr-smolt transformation. Erythrocytes appeared to be more sensitive to variation in dietary levels of TOCAA than liver and kidney tissues. Using the March ERPL assay results as a baseline, a TOCAA level of approximately 350-600 mg/kg diet would provide adequate protection against lipid peroxidation under most circumstances in juvenile Chinook salmon. ?? 2008 The Authors.

Journal of Animal Physiology and Animal Nutrition↗

USGS1989

Applicability of ambient toxicity testing to national or regional water-quality assessment

Comprehensive assessment of the quality of natural waters requires a multifaceted approach. Based on experimentation designed to monitor responses of organisms to environmental stresses, toxicity testing may have diverse purposes in water quality assessments. These purposes may include identification that warrant further study because of poor water quality or unusual ecological features, verification of other types of monitoring, or assessment of contaminant effects on aquatic communities. A wide variety of toxicity test methods have been developed to fulfill the needs of diverse applications. The methods differ primarily in the full selections made relative to four characteristics: (1) test species, (2) endpoints (acute or chronic), (3) test enclosure type, and (4) test substance (toxicant) that functions as the environmental stress. Toxicity test approachs vary in their capacity to meet the needs of large-scale assessments of existing water quality. Ambient testing is more likely to meet these needs than are the procedures that call for exposure of the test organisms to known concentrations of a single toxicant. However, meaningful interpretation of ambient test results depend on the existence of accompanying chemical analysis of the ambient media. The ambient test substance may be water or sediments. Sediment tests have had limited application, but they are useful because of the fact that most toxicants tend to accumulate in sediments, and many test species either inhabit the sediments or are in frequent contact with them. Biochemical testing methods, which have been developing rapidly in recent years, are likely to be among the most useful procedures for large-scale water quality assessments. They are relatively rapid and simple, and more importantly, they focus on biochemical changes that are the initial responses of virtually all organisms to environmental stimuli. Most species are sensitive to relatively few toxicants and their sensitivities vary as conditions change. One of the most informative approaches for toxicity testing is to combine biochemical tests with other test methods in a ' battery or tests ' that is diversified enough to characterize different types of toxicants and different trophic levels. (Lantz-PTT)

Open-File Report↗

USGS2009

Evaluation of LiDAR-Acquired Bathymetric and Topographic Data Accuracy in Various Hydrogeomorphic Settings in the Lower Boise River, Southwestern Idaho, 2007

Elevation data in riverine environments can be used in various applications for which different levels of accuracy are required. The Experimental Advanced Airborne Research LiDAR (Light Detection and Ranging) - or EAARL - system was used to obtain topographic and bathymetric data along the lower Boise River, southwestern Idaho, for use in hydraulic and habitat modeling. The EAARL data were post-processed into bare earth and bathymetric raster and point datasets. Concurrently with the EAARL data collection, real-time kinetic global positioning system and total station ground-survey data were collected in three areas within the lower Boise River basin to assess the accuracy of the EAARL elevation data in different hydrogeomorphic settings. The accuracies of the EAARL-derived elevation data, determined in open, flat terrain, to provide an optimal vertical comparison surface, had root mean square errors ranging from 0.082 to 0.138 m. Accuracies for bank, floodplain, and in-stream bathymetric data had root mean square errors ranging from 0.090 to 0.583 m. The greater root mean square errors for the latter data are the result of high levels of turbidity in the downstream ground-survey area, dense tree canopy, and horizontal location discrepancies between the EAARL and ground-survey data in steeply sloping areas such as riverbanks. The EAARL point to ground-survey comparisons produced results similar to those for the EAARL raster to ground-survey comparisons, indicating that the interpolation of the EAARL points to rasters did not introduce significant additional error. The mean percent error for the wetted cross-sectional areas of the two upstream ground-survey areas was 1 percent. The mean percent error increases to -18 percent if the downstream ground-survey area is included, reflecting the influence of turbidity in that area.

Scientific Investigations Report↗

USGS2014

Responses of predatory invertebrates to seeding density and plant species richness in experimental tallgrass prairie restorations

In recent decades, agricultural producers and non-governmental organizations have restored thousands of hectares of former cropland in the central United States with native grasses and forbs. However, the ability of these grassland restorations to attract predatory invertebrates has not been well documented, even though predators provide an important ecosystem service to agricultural producers by naturally regulating herbivores. This study assessed the effects of plant richness and seeding density on the richness and abundance of surface-dwelling (ants, ground beetles, and spiders) and aboveground (ladybird beetles) predatory invertebrates. In the spring of 2006, twenty-four 55 m × 55 m-plots were planted to six replicates in each of four treatments: high richness (97 species typically planted by The Nature Conservancy), at low and high seeding densities, and low richness (15 species representing a typical Natural Resources Conservation Service Conservation Reserve Program mix, CP25), at low and high seeding densities. Ants, ground beetles, and spiders were sampled using pitfall traps and ladybird beetles were sampled using sweep netting in 2007–2009. The abundance of ants, ground beetles, and spiders showed no response to seed mix richness or seeding density but there was a significant positive effect of richness on ladybird beetle abundance. Seeding density had a significant positive effect on ground beetle and spider species richness and Shannon–Weaver diversity. These results may be related to differences in the plant species composition and relative amount of grass basal cover among the treatments rather than richness.

Nebraska↗

USGS2025

Mercury trophic transfer to a freshwater biosentinel: Quantifying controlled bioaccumulation in larval dragonflies

Mercury bioavailability and biomagnification in freshwater systems can be highly variable; thus, tissue data from biosentinel taxa can be useful to assess risk. Dragonfly larvae have emerged as biological indicators of mercury impairment, yet their mercury biodynamics over time and across exposure levels are not well understood. Evaluating these attributes using controlled experimental approaches is an important step to validate larval dragonflies as biosentinels for spatial and temporal trends in mercury risk. We conducted an experimental series quantifying methylmercury trophic transfer from dosed prey to predatory dragonfly larvae at environmentally relevant concentrations. Dragonfly total mercury concentrations increased proportionally by factors of 2.7 to 4.2 with each doubling of prey methylmercury concentration, responding to dietary treatments in 7–28 days and reaching equilibrium in as little as 40 days, supporting their utility to indicate changing mercury exposure regimes. Dosed dragonflies biomagnified methylmercury by factors of 1.0 ± 0.1 to 3.4 ± 0.2 relative to their prey, and biomagnification efficiency decreased by over 40% for each doubling of prey methylmercury concentration. Dragonfly development had dose-dependent effects on bioaccumulation: mercury concentrations increased with growth and decreased with age in higher exposure treatments, whereas they decreased with growth and increased with age in lower exposure treatments. Bioaccumulation also varied taxonomically; within treatments, mean mercury concentrations for each genus varied up to 10% from family-level means. Dragonfly sex, size, and body condition did not significantly affect mercury concentrations. These results help validate and expand the utility of dragonfly larvae as biosentinels to monitor mercury risk and better protect wildlife and human health.

Oregon↗

USGS2007

Progressive oxidation of pyrite in five bituminous coal samples: An As XANES and 57 Fe Mössbauer spectroscopic study

Naturally occurring pyrite commonly contains minor substituted metals and metalloids (As, Se, Hg, Cu, Ni, etc.) that can be released to the environment as a result of its weathering. Arsenic, often the most abundant minor constituent in pyrite, is a sensitive monitor of progressive pyrite oxidation in coal. To test the effect of pyrite composition and environmental parameters on the rate and extent of pyrite oxidation in coal, splits of five bituminous coal samples having differing amounts of pyrite and extents of As substitution in the pyrite, were exposed to a range of simulated weathering conditions over a period of 17 months. Samples investigated include a Springfield coal from Indiana (whole coal pyritic S = 2.13 wt.%; As in pyrite = detection limit (d.l.) to 0.06 wt.%), two Pittsburgh coal samples from West Virginia (pyritic S = 1.32–1.58 wt.%; As in pyrite = d.l. to 0.34 wt.%), and two samples from the Warrior Basin, Alabama (pyritic S = 0.26–0.27 wt.%; As in pyrite = d.l. to 2.72 wt.%). Samples were collected from active mine faces, and expected differences in the concentration of As in pyrite were confirmed by electron microprobe analysis. Experimental weathering conditions in test chambers were maintained as follows: (1) dry Ar atmosphere; (2) dry O 2 atmosphere; (3) room atmosphere (relative humidity &sim;20–60%); and (4) room atmosphere with samples wetted periodically with double-distilled water. Sample splits were removed after one month, nine months, and 17 months to monitor the extent of As and Fe oxidation using As X-ray absorption near-edge structure (XANES) spectroscopy and 57 Fe Mössbauer spectroscopy, respectively. Arsenic XANES spectroscopy shows progressive oxidation of pyritic As to arsenate, with wetted samples showing the most rapid oxidation. 57 Fe Mössbauer spectroscopy also shows a much greater proportion of Fe 3+ forms (jarosite, Fe 3+ sulfate, FeOOH) for samples stored under wet conditions, but much less difference among samples stored under dry conditions in different atmospheres. The air-wet experiments show evidence of pyrite re-precipitation from soluble ferric sulfates, with As retention in the jarosite phase. Extents of As and Fe oxidation were similar for samples having differing As substitution in pyrite, suggesting that environmental conditions outweigh the composition and amount of pyrite as factors influencing the oxidation rate of Fe sulfides in coal.

Applied Geochemistry↗

USGS2005

Functional classification of mitochondrion-rich cells in euryhaline Mozambique tilapia (Oreochromis mossambicus) embryos, by means of triple immunofluorescence staining for Na+/K+-ATPase, Na +/K+/2Cl- cotransporter and CFTR anion channel

Mozambique tilapia Oreochromis mossambicus embryos were transferred from freshwater to seawater and vice versa, and short-term changes in the localization of three major ion transport proteins, Na+/K +-ATPase, Na+/K+/2Cl- cotransporter (NKCC) and cystic fibrosis transmembrane conductance regulator (CFTR) were examined within mitochondrion-rich cells (MRCs) in the embryonic yolk-sac membrane. Triple-color immunofluorescence staining allowed us to classify MRCs into four types: type I, showing only basolateral Na+/K +-ATPase staining; type II, basolateral Na+/K +-ATPase and apical NKCC; type III, basolateral Na+/K +-ATPase and basolateral NKCC; type IV, basolateral Na +/K+-ATPase, basolateral NKCC and apical CFTR. In freshwater, type-I, type-II and type-III cells were observed. Following transfer from freshwater to seawater, type-IV cells appeared at 12 h and showed a remarkable increase in number between 24 h and 48 h, whereas type-III cells disappeared. When transferred from seawater back to freshwater, type-IV cells decreased and disappeared at 48 h, type-III cells increased, and type-II cells, which were not found in seawater, appeared at 12 h and increased in number thereafter. Type-I cells existed consistently irrespective of salinity changes. These results suggest that type I is an immature MRC, type II is a freshwater-type ion absorptive cell, type III is a dormant type-IV cell and/or an ion absorptive cell (with a different mechanism from type II), and type IV is a seawater-type ion secretory cell. The intracellular localization of the three ion transport proteins in type-IV cells is completely consistent with a widely accepted model for ion secretion by MRCs. A new model for ion absorption is proposed based on type-II cells possessing apical NKCC.

Journal of Experimental Biology↗

USGS2020

Climate change causes river network contraction and disconnection in the H.J. Andrews Experimental Forest, Oregon, USA

Headwater streams account for more than 89% of global river networks and provide numerous ecosystem services that benefit downstream ecosystems and human water uses. It has been established that changes in climate have shifted the timing and magnitude of observed precipitation, which, at specific gages, have been directly linked to long-term reductions in large river discharge. However, climate impacts on ungaged headwater streams, where ecosystem function is tightly coupled to flow permanence along the river corridor, remain unknown due to the lack of data sets and ability to model and predict flow permanence. We analyzed a network of 10 gages with 38–69 years of records across a 5th-order river basin in the U.S. Pacific Northwest, finding increasing frequency of lower low-flow conditions across the basin. Next, we simulated river network expansion and contraction for a 65-year period of record, revealing 24% and 9% declines in flowing and contiguous network length, respectively, during the driest months of the year. This study is the first to mechanistically simulate network expansion and contraction at the scale of a large river basin, informing if and how climate change is altering connectivity along river networks. While the heuristic model presented here yields basin-specific conclusions, this approach is generalizable and transferable to the study of other large river basins. Finally, we interpret our model results in the context of regulations based on flow permanence, demonstrating the complications of static regulatory definitions in the face of non-stationary climate.

Oregon↗

USGS2010

Gypsies in the palace: Experimentalist's view on the use of 3-D physics-based simulation of hillslope hydrological response

As a fundamental unit of the landscape, hillslopes are studied for their retention and release of water and nutrients across a wide range of ecosystems. The understanding of these near-surface processes is relevant to issues of runoff generation, groundwater-surface water interactions, catchment export of nutrients, dissolved organic carbon, contaminants (e.g. mercury) and ultimately surface water health. We develop a 3-D physics-based representation of the Panola Mountain Research Watershed experimental hillslope using the TOUGH2 sub-surface flow and transport simulator. A recent investigation of sub-surface flow within this experimental hillslope has generated important knowledge of threshold rainfall-runoff response and its relation to patterns of transient water table development. This work has identified components of the 3-D sub-surface, such as bedrock topography, that contribute to changing connectivity in saturated zones and the generation of sub-surface stormflow. Here, we test the ability of a 3-D hillslope model (both calibrated and uncalibrated) to simulate forested hillslope rainfall-runoff response and internal transient sub-surface stormflow dynamics. We also provide a transparent illustration of physics-based model development, issues of parameterization, examples of model rejection and usefulness of data types (e.g. runoff, mean soil moisture and transient water table depth) to the model enterprise. Our simulations show the inability of an uncalibrated model based on laboratory and field characterization of soil properties and topography to successfully simulate the integrated hydrological response or the distributed water table within the soil profile. Although not an uncommon result, the failure of the field-based characterized model to represent system behaviour is an important challenge that continues to vex scientists at many scales. We focus our attention particularly on examining the influence of bedrock permeability, soil anisotropy and drainable porosity on the development of patterns of transient groundwater and sub-surface flow. Internal dynamics of transient water table development prove to be essential in determining appropriate model parameterization. ?? 2010 John Wiley & Sons, Ltd.

Hydrological Processes↗

USGS1994

Hydrologic control of litter decomposition in seasonally flooded prairie marshes

The effect of seasonal inundation on the decomposition of emergent macrophyte litter ( Scolochloa festucacea ) was examined under experimental flooding regimes in a northern prairie marsh. Stem and leaf litter was subjected to six aboveground inundation treatments (ranging from never flooded to flooded April through October) and two belowground treatments (nonflooded and flooded April to August). Flooding increased the rate of mass loss from litter aboveground but retarded decay belowground. Aboveground, N concentration decreased and subsequently increased earlier in the longer flooded treatments, indicating that flooding decreased the time that litter remained in the leaching and immobilization phases of decay. Belowground, both flooded and nonflooded litter showed an initial rapid loss of N, but concentration and percent of original N remaining were greater in the nonflooded marsh throughout the first year. This suggested that more N was immobilized on litter under the nonflooded, more oxidizing soil conditions. Both N concentration and percent N remaining of belowground litter were greater in the flooded than the nonflooded marsh the second year, suggesting that N immobilization was enhanced after water-level drawdown. These results suggest different mechanisms by which flooding affects decomposition in different wetland environments. On the soil surface where oxygen is readily available, flooding accelerates decomposition by increasing moisture. Belowground, flooding creates anoxic conditions that slow decay. The typical hydrologic pattern in seasonally flooded prairie marshes of spring flooding followed by water-level drawdown in summer may maximize system decomposition rates by allowing rapid decomposition aboveground in standing water and by annually alleviating soil anoxia.

Manitoba↗

USGS2016

Experimental evaluation of four ground-motion scaling methods for dynamic response-history analysis of nonlinear structures

This paper experimentally evaluates four methods to scale earthquake ground-motions within an ensemble of records to minimize the statistical dispersion and maximize the accuracy in the dynamic peak roof drift demand and peak inter-story drift demand estimates from response-history analyses of nonlinear building structures. The scaling methods that are investigated are based on: (1) ASCE/SEI 7–10 guidelines; (2) spectral acceleration at the fundamental (first mode) period of the structure, S a ( T 1 ); (3) maximum incremental velocity, MIV ; and (4) modal pushover analysis. A total of 720 shake-table tests of four small-scale nonlinear building frame specimens with different static and dynamic characteristics are conducted. The peak displacement demands from full suites of 36 near-fault ground-motion records as well as from smaller “unbiased” and “biased” design subsets (bins) of ground-motions are included. Out of the four scaling methods, ground-motions scaled to the median MIV of the ensemble resulted in the smallest dispersion in the peak roof and inter-story drift demands. Scaling based on MIV also provided the most accurate median demands as compared with the “benchmark” demands for structures with greater nonlinearity; however, this accuracy was reduced for structures exhibiting reduced nonlinearity. The modal pushover-based scaling (MPS) procedure was the only method to conservatively overestimate the median drift demands.

Bulletin of Earthquake Engineering↗

USGS2010

Seasonal ice and hydrologic controls on dissolved organic carbon and nitrogen concentrations in a boreal-rich fen

[1] Boreal wetland carbon cycling is vulnerable to climate change in part because hydrology and the extent of frozen ground have strong influences on plant and microbial functions. We examined the response of dissolved organic carbon (DOC) and total dissolved nitrogen (TDN) across an experimental manipulation of water table position (both raised and lowered water table treatments) in a boreal-rich fen in interior Alaska. DOC and TDN responses to water table manipulation exhibited an interaction with seasonal ice dynamics. We observed consistently higher DOC and TDN concentrations in the lowered water table treatment (71.7 ± 6.5 and 3.0 ± 0.3 mg−L) than in both the control (55.6 ± 5.1 and 2.3 ± 0.2 mg−L) and raised (49.1 ± 4.3 and 1.9 ± 0.1 mg L−1, respectively) water table treatments. Across all plots, pore water DOC concentrations at 20 cm increased as the depth to water table increased (R2 = 0.43, p < 0.001). DOC concentrations also increased as the seasonal thaw depth increased, with solutes increasing most rapidly in the drained plot (R2 = 0.62, p < 0.001). About half of the TDN pool was composed of dissolved organic N (DON). Inorganic N and DON were both highly correlated with changes in DOC, and their respective constraints to mineralization are discussed. These results demonstrate that a declining water table position and dryer conditions affect thaw depth and peat temperatures, and interactions among these ecosystem properties will likely increase DOC and TDN loading and potential for export in these systems.

Alaska↗

USGS2019

Nest-defense behavior of Mississippi Kites in urban and exurban areas

Mississippi kites ( Ictinia mississippiensis ) have become an abundant raptor in many urban and exurban areas throughout the Southern Great Plains of the United States. Unfortunately, human–wildlife conflicts have resulted from this juxtaposition of suitable breeding areas for kites and areas that humans frequent, with some kites responding aggressively to humans near nests. To date, there are no data describing the prevalence of aggressive nest defense in the species, making informed management of human and kite conflicts difficult. We assessed and compared the prevalence of aggressive nest-defense by Mississippi kites in an urban area and an exurban area by simulating nest disturbance with a trial pedestrian. Additionally, we examine the relationships between physical features of the nest tree where aggressive behaviors were and were not recorded. Individual kites breeding in the exurban area responded to the trial pedestrian by taking flight from the nesting area, circling overhead, swooping at the pedestrian, or remaining on the nest. In the urban area, kites displayed a more limited suit of responses and either remained on the nest or swooped at the pedestrian. Additionally, kites breeding in the exurban area appeared to respond to experimental disturbance at a greater distance than did urban breeding kites, but not with more attacks on pedestrians. Physical characteristics of the nest tree did not explain aggressive behaviors, thereby suggesting that aggression in Mississippi kites is caused by factors other than nesting location features.

Texas↗

USGS2026

Response of juvenile Chinook Salmon to an experimental reservoir operation to facilitate downstream migration

Objectives High-head dams and storage reservoirs impact ecological processes, impeding recovery efforts of Pacific salmon Oncorhynchus spp. in the western United States. These projects can delay migration, increase risks of predation and disease, and induce mortality for juvenile Pacific salmon during downstream passage. To mitigate these negative effects, large-scale changes to dam operations have been proposed as interim measures or alternatives to engineered fish passage solutions. A novel deep drawdown operation of the Lookout Point Project (two dams and reservoirs) in Oregon serves as a case study for learning from a large-scale management experiment. Beginning in July 2024, Lookout Point Reservoir was slowly lowered to attain minimal levels from mid-November to mid-December with the intent of expediting downstream passage through the project for juvenile Chinook Salmon Oncorhynchus tshawytscha . We evaluated the impacts of the months-long operation on two life histories of juvenile Chinook Salmon with different rearing environments and growth rates. Methods Acoustic telemetry was used to monitor juvenile Chinook Salmon in the Lookout Point Project. Environmental data, including water temperature and hydrological conditions, were collected. We fitted a time-integrated migration survival model to these data to estimate the effects of management-influenced covariates and fish length on apparent survival and residence time in each reach of the Lookout Point Project. Results Larger fish had higher survival than smaller fish in each reach of the Lookout Point Project. Apparent survival through each dam of the Lookout Point Project was highest and forebay residence times lowest during the latter part of the drawdown (late November). However, early periods of the drawdown (September) were associated with a transition from a thermally stratified reservoir to a warm isothermal reservoir that negatively impacted reservoir survival. Conclusions The deep drawdown of the Lookout Point Project achieved the management intent to improve downstream passage for juvenile Chinook Salmon at the lowest reservoir elevations but also caused unintended changes in the reservoir rearing environment prior to full drawdown. Further research could resolve remaining uncertainties regarding the overall effectiveness of drawdown operations with respect to the population dynamics of different juvenile life history strategies. Understanding these factors would help optimize future management actions aimed at Pacific salmon recovery above the Lookout Point Project.

Oregon↗

USGS2011

Experimental infection of bats with Geomyces destructans causes white-nose syndrome

White-nose syndrome (WNS) has caused recent catastrophic declines among multiple species of bats in eastern North America. The disease's name derives from a visually apparent white growth of the newly discovered fungus Geomyces destructans on the skin (including the muzzle) of hibernating bats. Colonization of skin by this fungus is associated with characteristic cutaneous lesions that are the only consistent pathological finding related to WNS. However, the role of G. destructans in WNS remains controversial because evidence to implicate the fungus as the primary cause of this disease is lacking. The debate is fuelled, in part, by the assumption that fungal infections in mammals are most commonly associated with immune system dysfunction. Additionally, the recent discovery that G. destructans commonly colonizes the skin of bats of Europe, where no unusual bat mortality events have been reported, has generated further speculation that the fungus is an opportunistic pathogen and that other unidentified factors are the primary cause of WNS. Here we demonstrate that exposure of healthy little brown bats (Myotis lucifugus) to pure cultures of G. destructans causes WNS. Live G. destructans was subsequently cultured from diseased bats, successfully fulfilling established criteria for the determination of G. destructans as a primary pathogen. We also confirmed that WNS can be transmitted from infected bats to healthy bats through direct contact. Our results provide the first direct evidence that G. destructans is the causal agent of WNS and that the recent emergence of WNS in North America may represent translocation of the fungus to a region with a naive population of animals. Demonstration of causality is an instrumental step in elucidating the pathogenesis and epidemiology of WNS and in guiding management actions to preserve bat populations against the novel threat posed by this devastating infectious disease. ?? 2011 Macmillan Publishers Limited. All rights reserved.

Nature↗

USGS2011

Selectivity evaluation for two experimental gill-net configurations used to sample Lake Erie walleyes

We used length frequencies of captured walleyes Sander vitreus to indirectly estimate and compare selectivity between two experimental gill-net configurations used to sample fish in Lake Erie: (1) a multifilament configuration currently used by the Ohio Department of Natural Resources (ODNR) with stretched-measure mesh sizes ranging from 51 to 127 mm and a constant filament diameter (0.37 mm); and (2) a monofilament configuration with mesh sizes ranging from 38 to 178 mm and varying filament diameter (range = 0.20–0.33 mm). Paired sampling with the two configurations revealed that the catch of walleyes smaller than 250 mm and larger than 600 mm was greater in the monofilament configuration than in the multifilament configuration, but the catch of 250–600-mm fish was greater in the multifilament configuration. Binormal selectivity functions yielded the best fit to observed walleye catches for both gill-net configurations based on model deviances. Incorporation of deviation terms in the binormal selectivity functions (i.e., to relax the assumption of geometric similarity) further improved the fit to observed catches. The final fitted selectivity functions produced results similar to those from the length-based catch comparisons: the monofilament configuration had greater selectivity for small and large walleyes and the multifilament configuration had greater selectivity for mid-sized walleyes. Computer simulations that incorporated the fitted binormal selectivity functions indicated that both nets were likely to result in some bias in age composition estimates and that the degree of bias would ultimately be determined by the underlying condition, mortality rate, and growth rate of the Lake Erie walleye population. Before the ODNR switches its survey gear, additional comparisons of the different gill-net configurations, such as fishing the net pairs across a greater range of depths and at more locations in the lake, should be conducted to maintain congruence in the fishery-independent survey time series.

North American Journal of Fisheries Management↗