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At least 1,315 records · Page 73Linked to original sources

Evaluation of the groundwater-flow model for the Ohio River alluvial aquifer near Carrollton, Kentucky, updated to conditions in September 2010

The Ohio River alluvial aquifer near Carrollton, Ky., is an important water resource for the cities of Carrollton and Ghent, as well as for several industries in the area. The groundwater of the aquifer is the primary source of drinking water in the region and a highly valued natural resource that attracts various water-dependent industries because of its quantity and quality. This report evaluates the performance of a numerical model of the groundwater-flow system in the Ohio River alluvial aquifer near Carrollton, Ky., published by the U.S. Geological Survey in 1999. The original model simulated conditions in November 1995 and was updated to simulate groundwater conditions estimated for September 2010. The files from the calibrated steady-state model of November 1995 conditions were imported into MODFLOW-2005 to update the model to conditions in September 2010. The model input files modified as part of this update were the well and recharge files. The design of the updated model and other input files are the same as the original model. The ability of the updated model to match hydrologic conditions for September 2010 was evaluated by comparing water levels measured in wells to those computed by the model. Water-level measurements were available for 48 wells in September 2010. Overall, the updated model underestimated the water levels at 36 of the 48 measured wells. The average difference between measured water levels and model-computed water levels was 3.4 feet and the maximum difference was 10.9 feet. The root-mean-square error of the simulation was 4.45 for all 48 measured water levels. The updated steady-state model could be improved by introducing more accurate and site-specific estimates of selected field parameters, refined model geometry, and additional numerical methods. Collection of field data to better estimate hydraulic parameters, together with continued review of available data and information from area well operators, could provide the model with revised estimates of conductance values for the riverbed and valley wall, hydraulic conductivities for the model layer, and target water levels for future simulations. Additional model layers, a redesigned model grid, and revised boundary conditions could provide a better framework for more accurate simulations. Additional numerical methods would identify possible parameter estimates and determine parameter sensitivities.

Kentucky↗

Structures data collection for the national map using volunteered geographic information

The U.S. Geological Survey (USGS) has historically sponsored volunteered data collection projects to enhance its topographic paper and digital map products. This report describes one phase of an ongoing project to encourage volunteers to contribute data to The National Map using online editing tools. The USGS recruited students studying geographic information systems (GIS) at the University of Colorado Denver and the University of Denver in the spring of 2011 to add data on structures - manmade features such as schools, hospitals, and libraries - to four quadrangles covering metropolitan Denver. The USGS customized a version of the online Potlatch editor created by the OpenStreetMap project and populated it with 30 structure types drawn from the Geographic Names Information System (GNIS), a USGS database of geographic features. The students corrected the location and attributes of these points and added information on structures that were missing. There were two rounds of quality control. Student volunteers reviewed each point, and an in-house review of each point by the USGS followed. Nine-hundred and thirty-eight structure points were initially downloaded from the USGS database. Editing and quality control resulted in 1,214 structure points that were subsequently added to The National Map . A post-project analysis of the data shows that after student edit and peer review, 92 percent of the points contributed by volunteers met National Map Accuracy Standards for horizontal accuracy. Lessons from this project will be applied to later phases. These include: simplifying editing tasks and the user interfaces, stressing to volunteers the importance of adding structures that are missing, and emphasizing the importance of conforming to editorial guidelines for formatting names and addresses of structures. The next phase of the project will encompass the entire State of Colorado and will allow any citizen to contribute structures data. Volunteers will benefit from this project by engaging with their local geography and contributing to a national resource of topographic information that remains in the public domain for anyone to download.

Denver↗

Pathology and infectious agents of unionid mussels: A primer for pathologists in disease surveillance and investigation of mortality events

Freshwater mussels are one of the most imperiled groups of organisms in the world, and more than 30 species have gone extinct in the last century. While habitat alteration and destruction have contributed to the declines, the role of disease in mortality events is unclear. In an effort to involve veterinary pathologists in disease surveillance and the investigation of freshwater mussel mortality events, we provide information on the conservation status of unionids, sample collection and processing techniques, and unique and confounding anatomical and physiological differences. We review the published accounts of pathology and infectious agents described in freshwater mussels including neoplasms, viruses, bacteria, fungi, fungal-like agents, ciliated protists, Aspidogastrea, Digenea, Nematoda, Acari, Diptera, and Odonata. Of the identified infectious agents, a single viral disease, Hyriopsis cumingii plague disease, that occurs only in cultured mussels is known to cause high mortality. Parasites including ciliates, trematodes, nematodes, mites, and insects may decrease host fitness, but are not known to cause mortality. Many of the published reports identify infectious agents at the light or ultrastructural microscopy level with no lesion or molecular characterization. Although metagenomic analyses provide sequence information for infectious agents, studies often fail to link the agents to tissue changes at the light or ultrastructural level or confirm their role in disease. Pathologists can bridge this gap between identification of infectious agents and confirmation of disease, participate in disease surveillance to ensure successful propagation programs necessary to restore decimated populations, and investigate mussel mortality events to document pathology and identify causality.

Veterinary Pathology↗

The Louisiana Amphibian Monitoring Program from 1997 to 2017: Results, analyses, and lessons learned

To determine trends in either frog distribution or abundance in the State of Louisiana, we reviewed and analyzed frog call data from the Louisiana Amphibian Monitoring Program (LAMP). The data were collected between 1997 and 2017 using North American Amphibian Monitoring Program protocols. Louisiana was divided into three survey regions for administration and analysis: the Florida Parishes, and 2 areas west of the Florida parishes called North and South. Fifty-four routes were surveyed with over 12,792 stops and 1,066 hours of observation. Observers heard 26 species of the 31 species reported to be in Louisiana. Three of the species not heard were natives with ranges that did not overlap with survey routes. The other two species were introduced species, the Rio Grande Chirping Frog ( Eleutherodactylus cystignathoides) and the Cuban Treefrog ( Osteopilus septentrionalis) . Both seem to be limited to urban areas with little to no route coverage. The 15 most commonly occurring species were examined in detail using the percentage of stops at which they observed along a given survey and their call indices. Most species exhibited a multimodal, concave, or convex pattern of abundance over a 15-year period. Among LAMP survey regions, none of the species had synchronous population trends. Only one group of species, winter callers, regularly co-occur. Based on the species lists, the North region could be seen as a subset of the South. However, based on relative abundance, the North was more similar to Florida parishes for both the winter and summer survey runs. Our analyses demonstrate that long-term monitoring (10 years or more) may be necessary to determine population and occupancy trends, and that frog species may have different local demographic patterns across large geographic areas.

Louisiana↗

Maximizing the water quality benefits of wetlands in croplands

Key Takeaways Nutrient loads from croplands continue to negatively affect surface water quality, despite considerable investments in and adoption of agricultural conservation practices aimed at reducing nutrient losses. Numerous studies indicate that effective restoration and management of wetlands in and adjacent to cultivated croplands could reduce surface and subsurface nutrient loads to downstream waters. Current drainage basin-scale models do not effectively account for the local-scale processes that are important in understanding the functional variability of wetlands and their potential as conservation practices across different spatial and temporal scales. Findings presented here from a literature review and simulation modeling study help inform bottom-up field-scale modeling of nitrogen and phosphorus dynamics and improve our understanding of the capacity for wetlands to provide nutrient retention services in agricultural drainage basins to inform strategic agricultural wetland restoration

Conservation Insight↗

Citing aquatic monitoring data sets: Best practice recommendations for authoritative data citation

The use of data generated from long term monitoring efforts necessitates accurate authoritative source citations of those data to ensure credit for data collected, and accountability for the data quality to enable repeated retrieval of a given data set. Data sets used in published reports and articles are increasingly being considered objects that are required to be published and cited. Aggregating data into open access databases is becoming common and is the focus of the Coordinated Assessment for Salmon and Steelhead project (CA; https://www.pnamp.org/project/coordinated-assessments-for-salmon-and-steelhead; http://www.streamnet.org/data/coordinated-assessments/ ) and National Marine Fisheries Service, National Oceanic and Atmospheric Administration Salmon Population Summary (SPS; https://www.webapps.nwfsc.noaa.gov/apex/f?p=261:home:0 ) among others. Guidelines are needed for citing these long-term dynamic data sets that have many contributors. We explore best practices and provide recommendations for including robust metadata attributes within data sets to enable data publication and citation using the CA and SPS data repositories as case studies. F rom reviewing the current citations possible from the CA and the SPS we recommend at minimum that natural resource monitoring databases contain: metadata to identify organizations that generated the data; contact persons for each organization that contributes data to an aggregated data set; and that metadata be incorporated into databases to enable auto-generated citations that recognize all contributing organizations with time-stamped versions of the data delivered. Beyond those minimums, additional best practice recommendations include this suite of metadata elements that identify a given data set upon citation or publication: author(s); publication date; description of data; file format(s) of data - e.g. tiles, shapefile sets, images, text files; dates data were collected; locations where data were collected; producers/contributors to the data set version cited; date data set was downloaded; original data repository from which the data were obtained; version identifier to note significant change to a data set; and a persistent identifier that can be used to locate that version of the data.

Report↗

Growth and development of thermoregulation in nestling San Miguel Island Song Sparrows

Patterns of growth (reviewed by Ricklefs 1968, 1969; O'Connor 1984) and the development of endothermy (reviewed by Dawson and Hudson 1970, Dunn 1975, Hill and Beaver 1982) have been well-studied in altricial wild birds, especially passerines. But few studies compare grown and thermogenesis in separate populations of the same species. Results of such studies with emberizids varied among species. King and Hubbard (1981), for example, found that nestlings from subarctic, subalpine, and low-altitude montane populations of White-crowned Sparrows ( Zonotrichia leucophrys gambelii ) grew at similar rates. In contrast, Rogers (1985) reported that the growth rates of nestlings in different populations of Savannah Sparrows ( Passerculus sandwichensis ) varied in response to the different environmental constraints of the localities in which they were reared. Nice (1937) and Smith et al. (1982) documented patterns of nestling growth in mainland (Ohio) and insular (Mandarte Island, British Columbia, Canada) populations of Song Sparrows ( Melospiza melodia ), respectively, and found that they were similar to those reported for most other passerines by Ricklefs (1968, 1969) and O'Connor (1984). In 1985-1986, we had the opportunity to examine the growth of nestlings from a third race of Song Sparrows, M. m. micronyx , which is endemic to San Miguel Island near Santa Barbara, California. We also studied the development of endothermy in these young birds, a process not hitherto described for nestling Song Sparrows. We report both in this paper.

California↗

Characterization methods for fractured glacial tills

This paper provides a literature review of methods successfully employed to characterize finegrained and fractured or unfractured glacial deposits. Descriptions and examples are given for four major categories of characterization methods: physical, hydraulic, chemical, and indirect. Characterization methods have evolved significantly within the past ten years; however, there still exists uncertainty about the reliability of individual characterization methods applied to till deposits. Therefore, a combination of methods is best, the choice of which depends on the objectives of the work. Sampling methods, sampling scales, and reporting methods are extremely important and should be considered when interpreting and comparing results between sites. Recognition of these issues is necessary to ensure that decisions regarding the transport of fluids in fractured tills are not based on the assumption that poorly permeable tills are always an inhibitor of subsurface flow.

Ohio Journal of Science↗

Multiple mortality events in bats: a global review

Despite conservation concerns for many species of bats, factors causing mortality in bats have not been reviewed since 1970. Here, we review and qualitatively describe trends in the occurrence and apparent causes of multiple mortality events (MMEs) in bats around the world. We compiled a database of MMEs, defined as cases in which ≥ 10 dead bats were counted or estimated at a specific location within a maximum timescale of a year, and more typically within a few days or a season. We tabulated 1180 MMEs within nine categories. Prior to 2000, intentional killing by humans caused the greatest proportion of MMEs in bats. In North America and Europe, people typically killed bats because they were perceived as nuisances. Intentional killing occurred in South America for vampire bat control, in Asia and Australia for fruit depredation control, and in Africa and Asia for human food. Biotic factors, accidents, and natural abiotic factors were also important historically. Chemical contaminants were confirmed causes of MMEs in North America, Europe, and in islands. Viral and bacterial diseases ranked low as causes of MMEs in bats. Two factors led to a major shift in causes of MMEs in bats at around 2000: the global increase of industrial wind-power facilities and the outbreak of white-nose syndrome in North America. Collisions with wind turbines and white-nose syndrome are now the leading causes of reported MMEs in bats. Collectively, over half of all reported MMEs were of anthropogenic origin. The documented occurrence of MMEs in bats due to abiotic factors such as intense storms, flooding, heat waves, and drought is likely to increase in the future with climate change. Coupled with the chronic threats of roosting and foraging habitat loss, increasing mortality through MMEs is unlikely to be compensated for, given the need for high survival in the dynamics of bat populations.

Mammal Review↗

It’s complicated and it depends: A review of the effects of ecosystem changes on Walleye and Yellow Perch populations in North America

Walleye Sander vitreus and Yellow Perch Perca flavescens are culturally, economically, and ecologically significant fish species in North America that are affected by drivers of global change. Here, we review and synthesize the published literature documenting the effects of ecosystem changes on Walleye and Yellow Perch. We focus on four drivers: climate (including temperature and precipitation), aquatic invasive species, land use and nutrient loading, and water clarity. We identified 1,232 tests from 370 papers, split evenly between Walleye ( n = 613) and Yellow Perch ( n = 619). Climate was the most frequently studied driver ( n = 572), and growth or condition was the most frequently studied response ( n = 297). The most commonly reported relationship was “no effect” (42% of analyses), usually because multiple variables were tested and only a few were found to be significant. Overall responses varied among studies for most species-response–driver combinations. For example, the influence of invasive species on growth of both Walleye and Yellow Perch was approximately equally likely to be positive, negative, or have no effect. Even when results were variable, important patterns emerged; for example, growth responses of both species to temperature were variable, but very few negative responses were observed. A few relationships were relatively consistent across studies. Invasive species were negatively associated with Walleye recruitment and abundance, and higher water clarity was negatively associated with Walleye abundance, biomass, and production. Some variability in responses may be due to differences in methodology or the range of variables studied; others represent true context dependence, where the effect of a driver depends on the influence of other variables. Using common metrics of impact, publishing negative results, and robust analytical approaches could facilitate comparisons among systems and provide a more comprehensive understanding of the responses of Walleye and Yellow Perch to ecosystem change.

North American Journal of Fisheries Management↗

Have humans influenced volcanic activity on the lower East Rift Zone of Kīlauea Volcano? A publication review

Since the 2018 eruption of Kīlauea Volcano, the topic of whether commercial developments not only caused the eruption to occur in the lower East Rift Zone (LERZ), but also caused its high eruption rate has been a subject of public discussion. We review Kīlauea Volcano publications from the past several decades and show that the eruptive behavior of the volcano has varied and that the 2018 eruption was similar to past eruptions in many ways. We find no evidence to support any human influence on Kīlauea Volcano.

Hawaii↗

Near-real-time information products for Mount St. Helens -- tracking the ongoing eruption

The rapid onset of energetic seismicity on September 23, 2004, at Mount St. Helens caused seismologists at the Pacific Northwest Seismic Network and the Cascades Volcano Observatory to quickly improve and develop techniques that summarized and displayed seismic parameters for use by scientists and the general public. Such techniques included webicorders (Web-based helicorder-like displays), graphs showing RSAM (real-time seismic amplitude measurements), RMS (root-mean-square) plots, spectrograms, location maps, automated seismic-event detectors, focal mechanism solutions, automated approximations of earthquake magnitudes, RSAM-based alarms, and time-depth plots for seismic events. Many of these visual-information products were made available publicly as Web pages generated and updated routinely. The graphs and maps included short written text that explained the concepts behind them, which increased their value to the nonseismologic community that was tracking the eruption. Laypeople could read online summaries of the scientific interpretations and, if they chose, review some of the basic data, thereby providing a better understanding of the data used by scientists to make interpretations about ongoing eruptive activity, as well as a better understanding of how scientists worked to monitor the volcano.

Washington↗

Attribution of monotonic trends and change points in peak streamflow across the conterminous United States using a multiple working hypotheses framework, 1941–2015 and 1966–2015

The U.S. Geological Survey has a long history of leading flood-frequency analysis studies. These studies play a critical role in the assessment of risk, protection of lives, and planning and design of flood protection infrastructure. Standard flood-frequency analysis is based on the assumption of stationarity—that is, that the distribution of floods at a given site varies around a particular mean within a particular envelope of variance (and skew) and that these parameters of the underlying statistical distribution representative of the floods do not vary over time. Gradual or abrupt changes in one or more of the distributional parameters are called nonstationarities and violate the underlying assumptions of current U.S. Federal Government guidelines for flood-frequency analysis. Uncertainty exists as to what degree of violations calls for the use of a modified method for flood-frequency analysis and what the modified method(s) should be. When deciding whether to perform nonstationary flood-frequency analysis and choosing a method for such analysis, it is important to understand the causes of the nonstationarity. Gradual or abrupt changes in distributional properties of floods may be the result of numerous factors, such as regulation, diversion, land-use change, or climate change. In the interest of developing a cohesive national approach for better understanding the causes of nonstationarities and incorporating potential or observed changes into flood-frequency estimates, subject-matter experts from the U.S. Geological Survey and cooperators worked together to develop a multiple working hypotheses framework for making attributions and a common vocabulary for making provisions of confidence. Seven regional teams of these experts used ancillary datasets and institutional knowledge to evaluate plausible causes for monotonic trends and change points in annual peak-streamflow data for the conterminous United States that had been identified in an earlier phase of the project. The first chapter of this professional paper describes the development of a list of the potential attributions, presents a literature review of the potential attributions, describes the regional approach, summarizes insights obtained from the attribution process, and suggests future research. The other chapters provide the methods used for attribution in the seven regions—Pacific Northwest, Upper Plains, Midwest, Northeast, Southwest, South-Central, and Southeast—and summarize the regional patterns of nonstationarities.

Conterminous United States↗

The Moloka‘i coral reef today, and alternatives for the future: Summary in The coral reef of south Moloka‘i, Hawai‘i—Portrait of a sediment-threatened fringing reef

From the contributions collected in this publication have emerged two important observations that have significance locally, nationally, and internationally. First, the fringing coral reef along the south coast of Moloka'i is one of the most extensive and luxuriant reefs in the eight main Hawaiian Islands. It is longer and more continuous and has denser coral cover than reefs at any of the other islands—this alone makes it a state and national treasure worthy of study and protection. The second observation is more sobering: sections of the south Moloka'i reef have been damaged in the past by sedimentation ultimately caused by human activities in adjacent watersheds. Although some of those activities are no longer taking place, their lingering effects are still being felt in the form of excess sediment runoff. We here review the basis for each of these observations, discuss how sedimentation affects the reef, and summarize the choices that are faced by the people of Moloka'i and the State of Hawai'i with regard to the reef’s future.

Hawai‘i↗

The flora of Oktibbeha County, Mississippi

We surveyed the flora of Oktibbeha County, Mississippi, U.S.A., from February 1994 to 1996. Occupying 118 square kilometers in east-central Mississippi, Oktibbeha County lies among 3 physiographic regions that include, from west to east, Interior Flatwoods, Pontotoc Ridge, and Black Prairie. Accordingly, the county harbors a diverse flora. Based on field work, as well as an extensive review of published literature and herbarium records at IBE and MISSA, we recorded a total of 1,148 taxa (1,125 species, 7 hybrids, 16 infraspecific taxa) belonging to 514 genera in 160 families, over 85% of all taxa documented were native. Compared to 3 other counties in east-central Mississippi, Oktibbeha County has the second largest recorded flora. The number of state-listed (endangered, threatened, or of special concern) taxa (67) documented in this survey far exceeds that reported from any other county in the region. Three introduced species, Ilex cornuta Lindl. & Paxton, Mahonia bealei (Fortune) Carrie??re, and Nandina domestica Thunb., are reported in a naturalized state for the first time from Mississippi. We also describe 16 different plant communities belonging to 5 broad habitat categories: bottomland forests, upland forests and prairies, aquatic habitats, seepage areas, and human-influenced habitats. A detailed description of the vegetation associated with each of these communities is provided.

SIDA, Contributions to Botany↗

Evidence for competitive dominance of Pink salmon (Oncorhynchus gorbuscha) over other Salmonids in the North Pacific Ocean

Relatively little is known about fish species interactions in offshore areas of the world's oceans because adequate experimental controls are typically unavailable in such vast areas. However, pink salmon (Oncorhynchus gorbuscha) are numerous and have an alternating-year pattern of abundance that provides a natural experimental control to test for interspecific competition in the North Pacific Ocean and Bering Sea. Since a number of studies have recently examined pink salmon interactions with other salmon, we reviewed them in an effort to describe patterns of interaction over broad regions of the ocean. Research consistently indicated that pink salmon significantly altered prey abundance of other salmon species (e.g., zooplankton, squid), leading to altered diet, reduced total prey consumption and growth, delayed maturation, and reduced survival, depending on species and locale. Reduced survival was observed in chum salmon (O. keta) and Chinook salmon (O. tshawytscha) originating from Puget Sound and in Bristol Bay sockeye salmon (O. nerka). Growth of pink salmon was not measurably affected by other salmon species, but their growth was sometimes inversely related to their own abundance. In all marine studies, pink salmon affected other species through exploitation of prey resources rather than interference. Interspecific competition was observed in nearshore and offshore waters of the North Pacific Ocean and Bering Sea, and one study documented competition between species originating from different continents. Climate change had variable effects on competition. In the North Pacific Ocean, competition was observed before and after the ocean regime shift in 1977 that significantly altered abundances of many marine species, whereas a study in the Pacific Northwest reported a shift from predation- to competition-based mortality in response to the 1982/1983 El Nino. Key traits of pink salmon that influenced competition with other salmonids included great abundance, high consumption rates and rapid growth, degree of diet overlap or consumption of lower trophic level prey, and early migration timing into the ocean. The consistent pattern of findings from multiple regions of the ocean provides evidence that interspecific competition can significantly influence salmon population dynamics and that pink salmon may be the dominant competitor among salmon in marine waters. ?? Springer 2005.

Reviews in Fish Biology and Fisheries↗

Biological and geochemical data along Indian Point, Vermilion Bay, Louisiana

Scientists from the U.S. Geological Survey, St. Petersburg Coastal and Marine Science Center collected shallow sediment cores and surface samples from a coastal salt marsh environment next to Vermilion Bay in southwest Louisiana in January 2013. The sampling was part of a larger USGS study to gather data for assessing environmental changes over the past 150 years. The objective of the study was to expand upon the historical context of sea level and storms affecting coastal systems and how these systems might change under persistent or varying conditions. The data from this report add to a regional environmental change database that aids with the continuing effort to understand the evolution of coastal systems. This report serves as an archive for sedimentological, radiochemical, and microbiological data derived from the sediment cores. Data are available for January 2013. Downloadable data are available as Excel spreadsheets and as JPEG files. Additional files include ArcGIS shapefiles of the sampling sites, detailed results of sediment analyses, and formal Federal Geographic Data Committee metadata. Acknowledgments The authors thank Nancy DeWitt, B.J. Reynolds, Christopher Reich (USGS, St. Petersburg Coastal and Marine Science Center), for help with sample collection and processing; Michael Ball, Sarai Piazza, and Gregory Steyer (USGS, Coastal Restoration Assessment Branch) for assistance accessing CRMS Site 541; and Darrell Anders and Phillip Turnipseed (USGS National Wetlands Research Center) for technical support while in the field. We would also like to thank Caitlyn Reynolds and Nicholas Zaremba for their pre-release commentary and peer review. Information Statement This publication was prepared by an agency of the United States Government. Although these data were processed successfully on a computer system at the U.S. Geological Survey, no warranty expressed or implied is made regarding the display or utility of the data on any other system, or for general or scientific purposes, nor shall the act of distribution imply any such warranty. The U.S. Geological Survey shall not be held liable for improper or incorrect use of the data described and (or) contained herein. Reference herein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise does not constitute or imply its endorsement, recommendation, or favoring by the United States Government or any agency

Louisiana↗

Histopathology of repeated, intermittent exposure of chloramine-T to walleye (Sander vitreum) and (Ictalurus punctalus) channel catfish

Chloramine-T (Cl-T) has been used safely and effectively to control bacterial gill disease in salmonids at a maximum exposure regimen of up to four consecutive, once-daily exposures administered for 60??min at 20??mg/L. However, data to document safe treatment concentrations of Cl-T are lacking for freshwater-reared fish other than salmonids. We report the histopathology resultant from the administration of 12 consecutive, once-daily, 180-min static immersion baths of 0, 20, 50, or 80??mg Cl-T/L to walleye (20????C) and channel catfish (27????C). Twelve fish of each species were euthanized immediately before the first exposure (initial controls) and then after the twelfth exposure and 7 and 14??days after the twelfth exposure. Only initial controls and fish euthanized immediately after the twelfth exposure were processed for histological review because of the general lack of exposure-related lesions in exposed fish. The only exposure-related histological changes were in the spleen where significantly greater erythrocyte swelling and necrosis was observed in channel catfish exposed at 80??mg/L relative to exposure at 0??mg/L; similar histological changes were insignificant for walleye, though there appeared to be a shift in the general category of histological change with degenerative changes (necrosis, etc.) observed following exposure at 50 or 80??mg/L compared to the inflammatory and hemodynamic changes (congestion, leukocyte infiltrate, etc.) observed in walleye exposed at 0 or 20??mg/L. The only significant change in peripheral blood cytology was that walleye fingerlings exposed at 80??mg/L had significantly fewer mature red blood cells and significantly more immature red blood cells per oil-immersion field than controls. The histopathological changes observed following exposure to Cl-T under an exaggerated exposure regimen suggest that walleye or channel catfish therapeutically exposed to Cl-T will not have treatment-related histological changes.

Aquaculture↗