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Variation in selective regimes drives intraspecific variation in life-history traits and migratory behaviour along an elevational gradient

Comparative studies, across and within taxa, have made important contributions to our understanding of the evolutionary processes that promote phenotypic diversity. Trait variation along geographic gradients provides a convenient heuristic for understanding what drives and maintains diversity. Intraspecific trait variation along latitudinal gradients is well-known, but elevational variation in the same traits is rarely documented. Trait variation along continuous elevational gradients, however, provides compelling evidence that individuals within a breeding population may experience different selective pressures. Our objectives were to quantify variation in a suite of traits along a continuous elevational gradient, evaluate whether individuals in the population experience different selective pressures along that gradient and quantify variation in migratory tendency along that gradient. We examined variation in a suite of 14 life-history, morphological and behavioural traits, including migratory tendency, of yellow-eyed juncos along a continuous 1000-m elevational gradient in the Santa Catalina Mountains of Arizona. Many traits we examined varied along the elevational gradient. Nest survival and nestling growth rates increased, while breeding season length, renesting propensity and adult survival declined, with increasing elevation. We documented the migratory phenotype of juncos (partial altitudinal migrants) and show that individual migratory tendency is higher among females than males and increases with breeding elevation. Our data support the paradigm that variation in breeding season length is a major selective pressure driving life-history variation along elevational gradients and that individuals breeding at high elevation pursue strategies that favour offspring quality over offspring quantity. Furthermore, a negative association between adult survival and breeding elevation and a positive association between nest survival and breeding elevation help explain both the downslope and reciprocal upslope seasonal migratory movements that characterize altitudinal migration in many birds. Our results demonstrate how detailed studies of intraspecific variation in suites of traits along environmental gradients can lend new insights into the evolutionary processes that promote diversification and speciation, the causes of migratory behaviour, and how animal populations will likely respond to climate change.

Journal of Animal Ecology↗

The efficacy of protoporphyrin as a predictive biomarker for lead exposure in canvasback ducks: effect of sample storage time

We used 363 blood samples collected from wild canvasback dueks ( Aythya valisineria ) at Catahoula Lake, Louisiana, U.S.A. to evaluate the effect of sample storage time on the efficacy of erythrocytic protoporphyrin as an indicator of lead exposure. The protoporphyrin concentration of each sample was determined by hematofluorometry within 5 min of blood collection and after refrigeration at 4 °C for 24 and 48 h. All samples were analyzed for lead by atomic absorption spectrophotometry. Based on a blood lead concentration of ≥0.2 ppm wet weight as positive evidence for lead exposure, the protoporphyrin technique resulted in overall error rates of 29%, 20%, and 19% and false negative error rates of 47%, 29% and 25% when hematofluorometric determinations were made on blood at 5 min, 24 h, and 48 h, respectively. False positive error rates were less than 10% for all three measurement times. The accuracy of the 24-h erythrocytic protoporphyrin classification of blood samples as positive or negative for lead exposure was significantly greater than the 5-min classification, but no improvement in accuracy was gained when samples were tested at 48 h. The false negative errors were probably due, at least in part, to the lag time between lead exposure and the increase of blood protoporphyrin concentrations. False negatives resulted in an underestimation of the true number of canvasbacks exposed to lead, indicating that hematofluorometry provides a conservative estimate of lead exposure.

Louisiana↗

Wet meadow regeneration through restoration of biophysical feedbacks

Wet meadows are globally significant ecosystems that provide critical hydrological, ecological, and biogeochemical functions, yet their extent has declined dramatically due to land use changes and hydrologic alteration. These sedge-dominated wetlands exist at the drier end of the wetland gradient, maintained by shallow groundwater and periodic inundation. This paper is a global synthesis of the ecological, geomorphic, and hydrological dynamics of wet meadows, with an emphasis on alluvial systems, to inform effective restoration strategies. We compare wet meadows to other wetlands, classify them into palustrine, lacustrine, and alluvial types, then focus on alluvial wet meadows and discuss how their formation and persistence depend on ground and surface water interactions, sediment deposition and flow obstructions, all mediated by biological processes. In particular, we highlight the role of hydric graminoids in resisting erosion and maintaining soil cohesion, how beaver promote meadow persistence, and the significance of wet meadows as carbon sinks. We also present stratigraphic evidence demonstrating that incision, often triggered by anthropogenic activity or changing climate, is the primary mechanism of alluvial wet meadow degradation, resulting in water table decline and shifts in vegetation composition. Restoration requires reversing these incisional processes through techniques that elevate water tables, disperse flow and retain sediment—methods traditionally associated with either soil conservation or stream restoration. These include nature-based solutions that create obstructions such as beaver dams and their analogues, rock and wood-based obstructions and incision trench or gully filling and grading. Given their multifunctional value—including but not limited to flood attenuation, biodiversity support, and carbon sequestration—wet meadows warrant a focused restoration framework. This review advocates for a valley-floor scale restoration paradigm that integrates hydrological reconnection, sediment retention, and biological reinforcement to ensure long-term resilience of these systems in the face of changing climate and land use pressures.

Frontiers in Environmental Science↗

Diverse migratory portfolios drive inter-annual switching behavior of elk across the Greater Yellowstone Ecosystem

A growing body of evidence shows that some ungulates alternate between migratory and nonmigratory behaviors over time. Yet it remains unclear whether such short-term behavioral changes can help explain reported declines in ungulate migration worldwide, as opposed to long-term demographic changes. Furthermore, advances in tracking technology reveal that a simple distinction between migration and nonmigration may not sufficiently describe all individual behaviors. To better understand the dynamics and drivers of ungulate switching behavior, we investigated 14 years of movement data from 361 elk in 20 herds across the Greater Yellowstone Ecosystem (GYE). First, we categorized yearly individual behaviors using a clustering algorithm that identified similar migratory tactics across a continuum of behaviors. Then, we tested seven hypotheses to explain why some ungulates switch behaviors, and we evaluated how behavioral changes affected the proportions of different behaviors across the system. We identified four distinct behavioral tactics: residents (4.8% of elk-years), short-distance migrants (53.7%), elevational migrants (21.9%) and long-distance migrants (19.6%). Of the 20 herds, 18 were partially migratory, and 5 had all four movement tactics present. We observed switches between migratory tactics in all sets of consecutive years during our study period, with an average of 22.5% of individual elk changing movement tactics from one year to the next. Elk in herds with higher movement tactic diversity were significantly more likely to switch tactics and often responded more effectively to adverse environmental conditions, compared to those in herds with low movement tactic diversity. During our study period, switching increased the prevalence of both short- and long-distance migrants, decreased the prevalence of elevational migrants, and had no effect on the prevalence of residents. Our findings suggest that rather than contributing to the declining migratory behavior found in the GYE, switching behavior may enable greater resiliency to continuously changing environmental and anthropogenic conditions.

Wyoming↗

Aquatic macroinvertebrates collected at Ravenna Army Ammunition Plant, Portage and Trumbull Counties, Ohio, 1998

The results of a survey of macroinvertebrate communities in the Ravenna Army Ammunition Plant, were used as an indicator of disturbance in streams flowing through or near the training areas at the Plant. The data were interpreted using the Invertebrate Community Index (ICI), a multiple-metric index developed by the Ohio Environmental Protection Agency and based on the structural and functional characteristics of the macroinvertebrate community. Quantitative samples of the macroinvertebrate were collected for ICI determination from three streams South Fork Eagle Creek, Sand Creek, and Hinkley Creek flowing through the study area. These samples were collected using Hester-Dendy type artificial substrate samplers, which were placed in the streams during a 6-week sampling period, June 2 through July 15, 1998. A qualitative- dipnet sample from the natural substrates also was collected at each station on July 15, 1998, the last day of the sampling period. The macroinvertebrate communities at all three stations met the criterion designated for warmwater habitat aquatic life use, and communities at two of the three stations exceeded the criterion. The ICI scores were 42 at South Fork Eagle Creek, 50 at Sand Creek, and 48 at Hinkley Creek. The density of macroinvertebrates at South Fork Eagle Creek was 1,245 per square foot and represented 38 distinct taxa. The density at Sand Creek was 246 per square foot and represented 29 distinct taxa. The density at Hinkley Creek was 864 per square foot and represented 36 distinct taxa. Qualitative samples were also collected at 21 other sites using a D-framed dipnet. The qualitative sites encompassed three main environments: stream, pond, and swamp-wetland. All available habitat types in each environment were sampled until no new taxa were evident during coarse examination. The highest number of taxa were collected from the streams. The total number of taxa collected in streams ranged from 25 to 76; the mean was 60 and median 64. The total taxa collected from ponds ranged from 32 to 60; the mean was 42 and median 41. The total taxa collected from swamp-wetland areas ranged from 6 to 30; the mean was 20 and median 23. The results are listed in phylogenetic order in this report and establish baseline data for future studies.

Ohio↗

Influence of groundwater recharge and well characteristics on dissolved arsenic concentrations in southeastern Michigan groundwater

Arsenic concentrations exceeding 10 ??g/l, the United States maximum contaminant level and the World Health Organization guideline value, are frequently reported in groundwater from bedrock and unconsolidated aquifers of southeastern Michigan. Although arsenic-bearing minerals (including arsenian pyrite and oxide/hydroxide phases) have been identified in Marshall Sandstone bedrock of the Mississippian aquifer system and in tills of the unconsolidated aquifer system, mechanisms responsible for arsenic mobilization and subsequent transport in groundwater are equivocal. Recent evidence has begun to suggest that groundwater recharge and characteristics of well construction may affect arsenic mobilization and transport. Therefore, we investigated the relationship between dissolved arsenic concentrations, reported groundwater recharge rates, well construction characteristics, and geology in unconsolidated and bedrock aquifers. Results of multiple linear regression analyses indicate that arsenic contamination is more prevalent in bedrock wells that are cased in proximity to the bedrock-unconsolidated interface; no other factors were associated with arsenic contamination in water drawn from bedrock or unconsolidated aquifers. Conditions appropriate for arsenic mobilization may be found along the bedrock-unconsolidated interface, including changes in reduction/oxidation potential and enhanced biogeochemical activity because of differences between geologic strata. These results are valuable for understanding arsenic mobilization and guiding well construction practices in southeastern Michigan, and may also provide insights for other regions faced with groundwater arsenic contamination. ?? Springer-Verlag 2008.

Environmental Geochemistry and Health↗

Evidence for the aquatic binding of arsenate by natural organic matter-suspended Fe(III)

Dialysis experiments with arsenate and three different NOM samples amended with Fe(III) showed evidence confirming the formation of aquatic arsenate-Fe(III)-NOM associations. A linear relationship was observed between the amount of complexed arsenate and the Fe(III) content of the NOM. The dialysis results were consistent with complex formation through ferric iron cations acting as bridges between the negatively charged arsenate and NOM functional groups and/or a more colloidal association, in which the arsenate is bound by suspended Fe(III)-NOM colloids. Sequential filtration experiments confirmed that a significant proportion of the iron present at all Fe/C ratios used in the dialysis experiments was colloidal in nature. These colloids may include larger NOM species that are coagulated by the presence of chelated Fe(III) and/or NOM-stabilized ferric (oxy)hydroxide colloids, and thus, the solution-phase arsenate-Fe(III)-NOM associations are at least partially colloidal in nature. ?? 2006 American Chemical Society.

Environmental Science & Technology↗

Tidal forested wetlands can be incorporated into blue carbon conservation and restoration strategies

Purpose of Review Blue carbon is an important concept for environmental policy. Blue carbon strategies (conservation and restoration for carbon gain) have been primarily implemented with mangroves, though are likely to be suitable for other tidal forested wetlands. Here, we discuss the expanding definition of blue carbon encompassing all tidal forested wetlands, synthesize ecological and carbon sink knowledge of tidal forested wetlands, and reflect on key actions in mangrove blue carbon research and implementation that could be applied to other tidal forested wetlands. Recent Findings Conceptually, the blue carbon concept has now expanded beyond traditional coastal vegetated ecosystems to include all tidal wetlands, including tidal forested wetlands. Emerging data on carbon sequestration, emissions, and budgets from around the world now show that many tidal forested wetland ecosystems are carbon sinks at a magnitude similar to mangroves. At the global scale, mangroves have become incorporated into blue carbon strategies rapidly compared to other tidal forested wetlands, facilitated by agenda-setting papers, adequate data addressing concerns on emissions and permanence, the availability of global maps, a clear ecosystem definition, clear accounting and policy frameworks, and international stakeholders who acted as high profile ecosystem advocates, alongside long-term capacity building efforts. This provides a roadmap for implementation in other tidal forested wetlands. Summary Tidal forested wetlands other than mangroves have high potential for blue carbon management. Many tidal forested wetlands share biophysical similarities with mangroves, carbon stocks can be similar, and methane emissions are often no higher. An increasing evidence base, challenging assumptions around greenhouse gas fluxes, and robust engagement with policy actors and frameworks, could increase the use of blue carbon for tidal forested wetland conservation and restoration.

Current Forestry Reports↗

Population genomics of free-ranging Great Plains white-tailed and mule deer reflects a long history of interspecific hybridization

Hybridization is a natural process at species-range boundaries that may variably promote the speciation process or break down species barriers but minimally will influence management outcomes of distinct populations. White-tailed deer ( Odocoileus virginianus ) and mule deer ( Odocoileus hemionus ) have broad and overlapping distributions in North America and a recognized capacity for interspecific hybridization. In response to contemporary environmental change to any of one or multiple still-unknown factors, mule deer range is contracting westward accompanied by a westward expansion of white-tailed deer, leading to increasing interactions, opportunities for gene flow, and associated conservation implications. To quantify genetic diversity, phylogenomic structure, and dynamics of hybridization in sympatric populations of white-tailed and mule deer, we used mitochondrial cytochrome b data coupled with SNP loci discovered with double-digest restriction site-associated DNA sequencing. We recovered 25,018 SNPs across 92 deer samples from both species, collected from two regions of western Kansas. Eight individuals with unambiguous external morphology representing both species were of hybrid origin (8.7%), and represented the product of multi-generational backcrossing. Mitochondrial data showed both ancient and recent directional discordance with morphological species assignments, reflecting a legacy of mule deer males mating with white-tailed deer females. Mule deer had lower genetic diversity than white-tailed deer, and both mitochondrial and nuclear data suggest contemporary mule deer effective population decline. Landscape genetic analyses show relative isolation between the two study regions for white-tailed deer, but greater connectivity among mule deer, with predominant movement from north to south. Collectively, our results suggest a long history of gene flow between these species in the Great Plains and hint at evolutionary processes that purge incompatible functional genomic elements as a result of hybridization. Surviving hybrids evidently may be reproductive, but with unknown consequences for the future integrity of these species, population trajectories, or relative susceptibility to emerging pathogens.

Kansas↗

Conservation of northwestern and southwestern pond turtles: Threats, population size estimates, and population viability analysis

Accurate status assessments of long-lived, widely distributed taxa depend on the availability of long-term monitoring data from multiple populations. However, monitoring populations across large temporal and spatial scales is often beyond the scope of any one researcher or research group. Consequently, wildlife managers may be tasked with utilizing limited information from different sources to detect range-wide evidence of population declines and their causes. When assessments need to be made under such constraints, the research and management communities must determine how to extrapolate from variable population data to species-level inferences. Here, using three different approaches, we integrate and analyze data from the peer-reviewed literature and government agency reports to inform conservation for northwestern pond turtles (NPT) Actinemys marmorata and southwestern pond turtles (SPT) Actinemys pallida . Both NPT and SPT are long-lived freshwater turtles distributed along the west coast of the United States and Mexico. Conservation concerns exist for both species; however, SPT may face more severe threats and are thought to exist at lower densities throughout their range than NPT. For each species, we ranked the impacts of 13 potential threats, estimated population sizes, and modeled population viability with and without long-term droughts. Our results suggest that predation of hatchlings by invasive predators, such as American bullfrogs Lithobates catesbeianus and Largemouth Bass Micropterus salmoides, is a high-ranking threat for NPT and SPT. Southwestern pond turtles may also face more severe impacts associated with natural disasters (droughts, wildfires, and floods) than do NPT. Population size estimates from trapping surveys indicate that SPT have smaller population sizes on average than do NPT ( P = 0.0003), suggesting they may be at greater risk of local extirpation. Population viability analysis models revealed that long-term droughts are a key environmental parameter; as the frequency of severe droughts increases with climate change, the likelihood of population recovery decreases, especially when census sizes are low. Given current population trends and vulnerability to natural disasters throughout their range, we suggest that conservation and recovery actions first focus on SPT to prevent further population declines.

Journal of Fish and Wildlife Management↗

National Pesticide Monitoring Program: Residues of organochlorine chemicals in freshwater fish, 1980–81

The U.S. Fish and Wildlife Service analyzed residues of organochlorine chemicals in 315 composite samples of whole fish collected in 1980–81 from 107 stations nationwide as part of the National Pesticide Monitoring Program (NPMP). The mean concentrations of total DDT and all p,p ′-ho-mologs except p,p ′-DDT showed significant but small declines relative to mean concentrations before 1978–79. The mean concentration of p,p ′-DDT did not change. The most persistent DDT homolog ( p,p ′-DDE) continued to constitute about 70% of total DDT residues, as it did in 1974–79. DDT contamination attributable to former DDT manufacturing facilities remained evident at sites in Alabama and Arkansas, and high concentrations at one site in Hawaii suggested that input to the environment was continuing. Conversely, the data do not support the contention of others that DDT was still being used in the Southwest. Residues of polychlorinated biphenyls (PCBs) remained widespread, but a significant downward trend in total PCBs was evident, especially in areas that the NPMP has previously shown to be the most heavily contaminated. Mean concentrations of dieldrin had not changed since 1978–79, and levels remained highest in Hawaii and in the Great Lakes. Toxaphene concentrations plateaued after a period of steady increase through the 1970's, but the incidence of toxaphene continued to increase; residues were present at 88% of the stations sampled in 1980–81, including one in Alaska (most likely as a consequence of atmospheric transport). Mean chlordane concentrations were slightly lower than in 1978–79, and residues were detected at fewer stations in 1980–81 than in 1978–79. Residues of other organochlorines [ e.g. , mirex, pentachloroanisole (PCA), benzene hexachloride (BHC) isomers, endrin, heptachlor, hexachlorobenzene (HCB), Dacthal ® (DCPA) 2 , and methoxychlor] were either found at relatively few (<25%) of the stations sampled in 1980–81 or were characterized by relatively low concentrations.

Archives of Environmental Contamination and Toxico↗

The impact of antecedent fire area on burned area in southern California coastal ecosystems

Frequent wildfire disasters in southern California highlight the need for risk reduction strategies for the region, of which fuel reduction via prescribed burning is one option. However, there is no consensus about the effectiveness of prescribed fire in reducing the area of wildfire. Here, we use 29 years of historical fire mapping to quantify the relationship between annual wildfire area and antecedent fire area in predominantly shrub and grassland fuels in seven southern California counties, controlling for annual variation in weather patterns. This method has been used elsewhere to measure leverage: the reduction in wildfire area resulting from one unit of prescribed fire treatment. We found little evidence for a leverage effect (leverage = zero). Specifically our results showed no evidence that wildfire area was negatively influenced by previous fires, and only weak relationships with weather variables rainfall and Santa Ana wind occurrences, which were variables included to control for inter-annual variation. We conclude that this is because only 2% of the vegetation burns each year and so wildfires rarely encounter burned patches and chaparral shrublands can carry a fire within 1 or 2 years after previous fire. Prescribed burning is unlikely to have much influence on fire regimes in this area, though targeted treatment at the urban interface may be effective at providing defensible space for protecting assets. These results fit an emerging global model of fire leverage which position California at the bottom end of a continuum, with tropical savannas at the top (leverage = 1: direct replacement of wildfire by prescribed fire) and Australian eucalypt forests in the middle (leverage ∼ 0.25).

California↗

Sea-floor character and sedimentary processes in the vicinity of Woods Hole, Massachusetts

Continuous-coverage multibeam bathymetric models and sidescan-sonar imagery have been verified with high-resolution seismic-reflection profiles, sediment sampling, and bottom photography. Together these data layers provide detailed base maps that yield topographic, compositional, and environmental perspectives of the sea floor in the vicinity of Woods Hole, an important harbor and major passage between the Elizabeth Islands and Cape Cod, Massachusetts. Tidally dominated high-energy environments within Woods Hole have prevented deposition of Holocene marine sediments, exposed underlying glacial drift of the Buzzards Bay moraine, and winnowed finer grained sediments, leaving lag deposits of boulders and gravel. These conditions have also enlarged and preserved depressions in the moraine surface that were originally kettle holes and formed ebb-tidal deltas at the entrances to passages. Fields of transverse and barchanoid sand waves dominate across the southern part of the study area in Vineyard Sound, where benthic environments are characterized by processes associated with coarse-bedload transport. Transverse sand waves dominate near shoals where sediment supply is greater and have asymmetries that indicate that the shoals are shaped and maintained by clockwise gyres of net sediment transport. Barchanoid sand waves, which are most common where Holocene sediments are thinner, commonly align into elongate fields that have smaller isolated waves concentrated at the eastern ends and that progressively widen and have waveforms that increase in amplitude, wavelength, and complexity westward. The northern, protected parts of the Little and Inner Harbors are characterized by muddy sediment and processes associated with deposition. A pockmark field in Little Harbor and the muddy, organic-rich sediments that form a scarp along the edge of Parker Flat are evidence for the presence of submerged marsh deposits formed during the Holocene rise in sea level.

Massachusetts↗

Integrating high-resolution coastal acidification monitoring data across seven United States estuaries

Beginning in 2015, the United States Environmental Protection Agency’s (EPA’s) National Estuary Program (NEP) started a collaboration with partners in seven estuaries along the East Coast (Barnegat Bay; Casco Bay), West Coast (Santa Monica Bay; San Francisco Bay; Tillamook Bay), and the Gulf of Mexico (GOM) Coast (Tampa Bay; Mission-Aransas Estuary) of the United States to expand the use of autonomous monitoring of partial pressure of carbon dioxide ( p CO 2 ) and pH. Analysis of high-frequency (hourly to sub-hourly) coastal acidification data including p CO 2 , pH, temperature, salinity, and dissolved oxygen (DO) indicate that the sensors effectively captured key parameter measurements under challenging environmental conditions, allowing for an initial characterization of daily to seasonal trends in carbonate chemistry across a range of estuarine settings. Multi-year monitoring showed that across all water bodies temperature and p CO 2 covaried, suggesting that p CO 2 variability was governed, in part, by seasonal temperature changes with average p CO 2 being lower in cooler, winter months and higher in warmer, summer months. Furthermore, the timing of seasonal shifts towards increasing (or decreasing) p CO 2 varied by location and appears to be related to regional climate conditions. Specifically, p CO 2 increases began earlier in the year in warmer water, lower latitude water bodies in the GOM (Tampa Bay; Mission-Aransas Estuary) as compared with cooler water, higher latitude water bodies in the northeast (Barnegat Bay; Casco Bay), and upwelling-influenced West Coast water bodies (Tillamook Bay; Santa Monica Bay; San Francisco Bay). Results suggest that both thermal and non-thermal influences are important drivers of p CO 2 in Tampa Bay and Mission-Aransas Estuary. Conversely, non-thermal processes, most notably the biogeochemical structure of coastal upwelling, appear to be largely responsible for the observed p CO 2 values in West Coast water bodies. The co-occurrence of high salinity, high p CO 2 , low DO, and low temperature water in Santa Monica Bay and San Francisco Bay characterize the coastal upwelling paradigm that is also evident in Tillamook Bay when upwelling dominates freshwater runoff and local processes. These data demonstrate that high-quality carbonate chemistry observations can be recorded from estuarine environments using autonomous sensors originally designed for open-ocean settings.

California, Florida, Maine, New Jersey, Oregon, Te↗

Book review: Proceedings of the First International Snakehead Symposium

Snakehead fishes (family Channidae) are among the most maligned aquatic invasive species in the USA and some other countries where they have been introduced outside of their native range in Asia and Africa. Nevertheless, snakeheads continue to be widely exploited in the live‐food trade in aquaculture and wild‐capture fisheries, are highly sought by anglers, and are also popular in the aquarium trade (Courtenay and Williams 2004). The Northern Snakehead Channa argus is the most widespread of the three channid species that are currently naturalized in the USA. This species has generated much concern and controversy, a situation that is partly fueled by sensational media coverage and B‐grade science fiction horror films, such as “Frankenfish,” “Snakehead Terror,” and “Snakehead Swamp.” Media reports of snakehead introductions are often replete with provocative terms, such as “vicious,” “villain,” “voracious,” “monster,” “diabolical,” and even “ecological Armageddon.” When snakeheads first appeared in natural waters of the USA, fisheries professionals became increasingly interested in their status. Established populations rapidly expanded in the mid‐Atlantic region and Arkansas, with scattered reports of introduced snakeheads from isolated locations in Hawaii, California, North Carolina, Florida, the Upper Midwest, and New England. In 2002, snakeheads were added to the list of injurious fishes under the Lacey Act, thereby prohibiting their importation or transport across state lines without a permit. This symposium was conceived by the editors and other concerned fisheries professionals of the Mississippi River Basin Panel on Aquatic Invasive Species. The mission of the symposium, held in Alexandria, Virginia, in July 2018, was to bring together experts on snakehead biology and ecology and to synthesize existing information into summary papers. In this book, 35 authors contributed to 15 peer‐reviewed articles that detail the current state of knowledge about snakehead introductions in the USA. Additionally, 16 abstracts are included from meeting presentations that were not accompanied by full‐length manuscripts. Also included is a summary of a facilitated symposium panel discussion featuring eight experts representing state and federal natural resource agencies and private fishing organizations. The book is organized into six sections. In the first section (Distribution), three papers provide an overview of the Channa species introduced into the USA and historical accounts of occurrence and dispersal of the Northern Snakehead in the mid‐Atlantic region and Arkansas. The second section (Biology/Ecology) consists of two articles that examine growth and energetics of Northern Snakehead populations and two papers that investigate diet, diel feeding activity, and movement of this species in the Potomac River drainage. The third section (Monitoring/Response) includes a paper that models range expansion of the Northern Snakehead in the southeastern USA based on occurrence data and environmental conditions. Also included in this section is a paper summarizing an environmental DNA study to assess the status and range of the Bullseye Snakehead C. marulius in southern Florida. The fourth section (Management/Control) is comprised of four papers that address harvest, age and growth, and development of a stock–recruitment model to inform management decisions regarding control and mitigation for Northern Snakehead populations in the greater Chesapeake Bay area. The fifth section (Perspectives) includes a paper on the history of snakehead introductions in Japan and a thought‐provoking social commentary on the human dimensions of Northern Snakehead management. Abstracts in the final section provide brief summaries of a diversity of snakehead studies, including aspects of distribution, ecology, behavior, control and monitoring efforts, public outreach, and pathology. The summary of the panel discussion is an engaging dialogue about the challenges of snakehead management in the context of conflicts regarding snakeheads as injurious versus their value as game and food species. Most of this book is focused on the Northern Snakehead. Much has been done to document snakehead distributions and certain aspects of snakehead biology, such as diets, age, and growth. Less research has been devoted to understanding the ecological impacts of snakeheads to native aquatic communities and ecosystems. This book would have benefited from a chapter summarizing the current systematics and diversity of the Channidae to inform fisheries biologists about the morphological characteristics of the family, approximate numbers of genera and species, and taxonomic instability. Exemplifying the latter, recent molecular and morphological evidence indicates uncertainty regarding identification of the feral snakehead population in Florida (Adamson and Britz 2019). Those authors suggest that this population may have originated from western Thailand, a possibility that could have implications for understanding historical pathways of snakehead introductions into the USA. In comparison with many published AFS symposia, this volume is relatively narrow in scope and lacks cohesive integration. It will primarily be of interest to those fisheries professionals engaged in the study of snakeheads as well as other nonnative species for which there are contrasting social values regarding their management: whether to monitor and attempt control or eradication efforts or to maintain populations for harvest as game or food species. The book should serve to identify information gaps and guide future research.

Transactions of the American Fisheries Society↗

Analysis of nitrate (NO3-N) concentration trends in 25 ground-water-quality management areas, Idaho, 1961-2001

In Idaho, drinking-water supplies are pumped from relatively shallow ground-water zones where water quality has great potential for degradation by land- and water-use activities. One indicator of water quality, and one of the most widespread contaminants in Idaho ground water related to land and water uses, is dissolved nitrate. In December 2000, the U.S. Geological Survey, in cooperation with the Idaho Department of Environmental Quality, began a study to compile and assess nitrate data for ground water in 25 ground-water-quality management areas in Idaho. The primary objective of the study was to determine whether statistically significant trends in ground-water concentrations were discernible. Data included ground-water analyses but not analyses of water from springs, drains, or thermal water sources. A total of 8,465 nitrate analyses were compiled from 2,931 wells in the 25 priority areas; analyses dates ranged from June 1961 to February 2001. A time-period comparison was used to assess general trends in nitrate concentrations within individual priority areas. A time-series comparison was used to assess trends in nitrate concentrations from selected wells in each priority area. In time-period assessments, general trends in an area were evaluated by compiling nitrate data for selected time intervals to determine whether the population distributions were significantly different between one period and another. Data within each priority area were sorted into decades— 1970s, 1980s, and 1990s—for long-term trend assessment. The 1990s data also were divided into sets of selected years corresponding to Statewide Ambient Ground-Water Quality Program sampling cycles—1991 through 1994, 1995 through 1998, and a partial cycle, 1999 through 2000—for shortterm trend assessment. Data were analyzed by summary statistics, boxplots, and the Mann- Whitney statistical test. Long-term increasing trends in nitrate concentrations were evident for 6 of 25 priority areas, and long-term decreasing trends were evident for 4 of 25 areas. Short-term increasing trends were evident for 7 of 25 areas, and a short-term decreasing trend was evident for only 1 area. No long-term nitrate trends were evident for 7 of 25 areas, and no shortterm trends were evident for 15 of 25 areas. Data were insufficient for long-term trend assessment in 8 areas and for short-term assessment in 2 areas. Time-series trend analyses were conducted on data from wells with 7 or more nitrate analyses and longest periods of record, at least 10 years between oldest and most recent analyses. Because long-term records were available for so few wells and well construction data were not available for several of these wells, time-series analyses were not helpful to the nitrate trend assessment study. Trend results may be strongly affected by well construction, hydrogeologic environments, and changes in density and areal distribution of wells and analyses. The utility of nitrate trend assessments in priority areas would be improved by more consistent and specific well location descriptions between agencies; well construction and major water-yielding zone information to accompany the water-quality data for each well; and addition of historical nitrate data to data bases, particularly analyses prior to about 1990. Investigations of the possible effects of changing priority area boundaries and time periods on both long-term and short-term trend assessments are needed. Addition of these kinds of information would allow assessment of trends associated with hydrology and geology of each area and would provide a much stronger basis for trend assessment than currently possible.

Idaho↗

Reproductive health and endocrine disruption in smallmouth bass (Micropterus dolomieu) from the Lake Erie drainage, Pennsylvania, USA

Smallmouth bass Micropterus dolomieu were sampled from three sites within the Lake Erie drainage (Elk Creek, Twentymile Creek, and Misery Bay, an embayment in Presque Isle Bay). Plasma, tissues for histopathological analyses, and liver and testes preserved in RNALater® were sampled from 30 smallmouth bass (of both sexes) at each site. Liver and testes samples were analyzed for transcript abundance with Nanostring nCounter® technology. Evidence of estrogenic endocrine disruption was assessed by the presence and severity of intersex (testicular oocytes; TO) and concentrations of plasma vitellogenin in male fish. Abundance of 17 liver transcripts associated with reproductive function, endocrine activity, and contaminant detoxification pathways and 40 testes transcripts associated with male and female reproductive function, germ cell development, and steroid biosynthesis were also measured. Males with a high rate of TO (87–100%) and plasma vitellogenin were noted at all sites; however, TO severity was greatest at the site with the highest agricultural land cover. Numerous transcripts were differentially regulated among the sites and patterns of transcript abundance were used to better understand potential risk factors for estrogenic endocrine disruption. The results of this study suggest endocrine disruption is prevalent in this region and further research would benefit to identify the types of contaminants that may be associated with the observed biological effects.

Pennsylvania↗

Widespread presence of naturally occurring perchlorate in high plains of Texas and New Mexico

Perchlorate (ClO4-) occurrence in groundwater has previously been linked to industrial releases and the historic use of Chilean nitrate fertilizers. However, recently a number of occurrences have been identified for which there is no obvious anthropogenic source. Groundwater from an area of 155 000 km2 in 56 counties in northwest Texas and eastern New Mexico is impacted by the presence of ClO4-. Concentrations were generally low (<4 ppb), although some areas are impacted by concentrations up to 200 ppb. ClO4- distribution is not related to well type (public water system, domestic, agricultural, or water-table monitoring) or aquifer (Ogallala, Edward Trinity High Plains, Edwards Trinity Plateau, Seymour, or Cenozoic). Results from vertically nested wells strongly indicate a surface source. The source of ClO4- appears to most likely be atmospheric deposition. Evidence supporting this hypothesis primarily relates to the presence of ClO 4- in tritium-free older water, the lack of relation between land use and concentration distribution, the inability of potential anthropogenic sources to account for the estimated mass of ClO4-, and the positive relationship between conserved anions (e.g., IO3-, Cl-, SO4-2) and ClO4-. The ClO4- distribution appears to be mainly related to evaporative concentration and unsaturated transport. This process has led to higher ClO4- and other ion concentrations in groundwater where the water table is relatively shallow, and in areas with lower saturated thickness. Irrigation may have accelerated this process in some areas by increasing the transport of accumulated salts and by increasing the number of evaporative cycles. Results from this study highlight the potential for ClO4- to impact groundwater in arid and semiarid areas through long-term atmospheric deposition. ?? 2006 American Chemical Society.

Environmental Science & Technology↗