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At least 1,153 records · Page 64Linked to original sources

Estimation of long-term discharge statistics by regional adjustment

A regional adjustment relationship was developed to estimate long-term (30-year) monthly median discharges from short term (three-year) records. This method differs from traditional approaches in that it is based on site-specific discharge data but does not require correlation of these data with discharges from a single hydrologically similar long-term gage. The method is shown to be statistically robust, and applicable to statistics other than the median.A regional adjustment relationship was developed to estimate long-term (30-year) monthly discharges from short term (three-year) records. This method differs from traditional approaches in that it is based on site-specific discharge data but does not require correlation of these data with discharges from a single hydrologically similar long-term gage. The method is shown to be statistically robust, and applicable to statistics other than the median.

Journal of the American Water Resources Associatio↗

Relative influence of streamflows in assessing temporal variability in stream habitat

The effects of streamflows on temporal variation in stream habitat were analyzed from the data collected 6-11 years apart at 38 sites across the United States. Multiple linear regression was used to assess the variation in habitat caused by streamflow at the time of sampling and high flows between sampling. In addition to flow variables, the model also contained geomorphic and land use factors. The regression model was statistically significant (p < 0.05; R 2 = 0.31-0.46) for 5 of 14 habitat variables: mean wetted stream depth, mean bankfull depth, mean wetted stream width, coefficient of variation of wetted stream width, and the percent frequency of bank erosion. High flows between samples accounted for about 16% of the total variation in the frequency of bank erosion. Streamflow at the time of sampling was the main source of variation in mean stream depth and contributed to the variation in mean stream width and the frequency of bank erosion. Urban land use (population change) accounted for over 20% of the total variation in mean bankfull depth, 15% of the total variation in the coefficient of variation of stream width, and about 10% of the variation in mean stream width. ?? 2007 American Water Resources Association.

Journal of the American Water Resources Associatio↗

Local weather and endogenous factors affect the initiation of migration in short- and medium-distance songbird migrants

Migratory birds employ a variety of mechanisms to ensure appropriate timing of migration based on integration of endogenous and exogenous information. The cues to fatten and depart from the non-breeding area are often linked to exogenous cues such as temperature or precipitation and the endogenous program. Shorter distance migrants should rely heavily on environmental information when initiating migration given relatively close proximity to the breeding area. However, the ability to fatten and subsequently depart may be linked to individual circumstances, including current fuel load and body size. For early and late departing migrants, we investigate effects of temperature, precipitation, lean body mass, fuel load and day of year on the initiation of migration (i.e. fuel load and departure timing) from the non-breeding region by analyzing 21 years of banding data for four species of short- and medium-distance migrants. Temperatures at the non-breeding area were related to temperatures at potential stopover areas. Despite local cues being predictive of conditions further north, the amount variation explained by local weather conditions in our models differed by species and temporal period but was low overall (< 33% variation explained). For each species, we also compared lean body mass and fuel load between early and late departing migrants, which showed mixed results. Our combined results suggest that most individuals migrating short or medium distances in our study did not time the initiation of migration with local predictive cues alone, but rather other factors such as lean body mass, fuel load, day of year, which may be a proxy for the endogenous program, and those beyond the scope of our study also influenced the initiation of migration. Our study contributes to understanding which factors influence departure decisions of short- and medium-distance migrants as they transition from the non-breeding to the migratory phase of the annual cycle.

Louisiana↗

Gulf Coast vicariance shapes phylogeographic history of a North American freshwater mussel species complex

Aim Freshwater mussels share habitat and are parasites of freshwater fishes during the larval life stage. Therefore, models of fish biogeography may also explain the historical biogeography of freshwater mussels. We tested this assumption using predictions of three biogeographic models constructed for northern Gulf of Mexico drainages on a freshwater mussel species complex. Specifically, we tested (1) if speciation was due to vicariant events of fluctuating sea levels that separated lineages east‐west of the Mobile Basin (Central Gulf Coast speciation hypothesis), (2) if the timing of divergences occurred 8.5–3.5 MYA (Gulf Coast allopatric speciation model) and (3) if diversification in Mississippi River populations was recent and for evidence of population increase consistent with range expansion into northern deglaciated regions (Pleistocene glaciation model). Location Eastern North America. Taxon Freshwater mussels (Bivalvia: Unionidae), Lampsilis teres and L. floridensis . Methods We collected 249 specimens from 73 localities across the group's distribution. We used three molecular markers (COI, NDI & ITSI) to conduct time calibrated Bayesian phylogenetic analyses, phylogeographic analyses (AMOVA & SAMOVA) and demographic analyses including Bayesian skyline plots. Results Lampsilis teres and L. floridensis are allopatric species whose distributions meet at the eastern edge of the Mobile Basin. Speciation was estimated to occur in the late Miocene. Populations from isolated river systems surrounding the Gulf of Mexico are almost all monophyletic. Mississippi drainage samples formed a shallow clade with recent diversification and showed evidence of recent population expansion. Main conclusions The historical biogeography of the L. teres species complex is broadly consistent with tested ichthyofaunal models. The timing of speciation and intraspecific divergences correspond to low sea‐level events suggesting that Gulf Coast sea‐level fluctuations are responsible for dispersal (sea‐level recession) and subsequent cladogenesis (sea‐level inundation). Consistent with numerous other freshwater studies, we found the Mobile Basin to be a suture zone, which may be due to the narrow, offshore continental shelf.

Journal of Biogeography↗

Complete and draft genome sequences of six members of the aquificales

The Aquificales are widespread in marine and terrestrial hydrothermal environments. Here, we report the complete and draft genome sequences of six new members of the Aquificales : two marine species, Persephonella marina strain EX-H1 and Hydrogenivirga strain 128-5-R1 (from the East Pacific Rise, 9°50.3′N, 104°17.5′W, and the Eastern Lau Spreading Center, 176°11.5′W, 20°45.8′S, respectively), and four terrestrial isolates, Sulfurihydrogenibium azorense strain Az-Fu1, Sulfurihydrogenibium yellowstonense strain SS-5, and Sulfurihydrogenibium strain Y03AOP1 (from Furnas, Azores, Portugal, and Calcite Springs and Obsidian Pool in Yellowstone National Park, United States, respectively), and the only thermoacidophilic isolate, Hydrogenobaculum strain Y04AAS1 (from a stream adjacent to Obsidian Pool). Significant differences among the different species exist that include nitrogen metabolism, hydrogen utilization, chemotaxis, and signal transduction, providing insights into their ecological niche adaptations.

Journal of Bacteriology↗

The role of virulence for in vivo superinfection fitness of a vertebrate RNA virus

We have developed a novel, in vivo superinfection fitness assay to examine superinfection dynamics and the role of virulence in superinfection fitness. This assay involves controlled, sequential infections of a natural, vertebrate host, Oncorhynchus mykiss (rainbow trout), with variants of a co-evolved viral pathogen, infectious hematopoietic necrosis virus (IHNV). Intervals between infections ranged from 12 hours to 7 days, and both frequency of superinfection and viral replication levels were examined. Using virus genotype pairs of equal and unequal virulence, we observed that superinfection generally occurred with decreasing frequency as the interval between exposures to each genotype increased. For both the equal virulence and unequal virulence genotype pairs, the frequency of superinfection was the same regardless of which genotype was used in the primary exposure. However, the ability to replicate in the context of superinfection did not differ between the genotypes of equal or unequal virulence tested here. For both genotype pairs, the mean viral load of the secondary virus was significantly reduced in superinfection, while primary virus replication was unaffected. Our results demonstrate, for the two genotype pairs examined, that superinfection restriction does occur for IHNV, and that higher virulence did not correlate with a significant difference in superinfection fitness. To our knowledge, this is the first assay to examine the role of virulence of an RNA virus in determining superinfection fitness dynamics within a natural, vertebrate host.

Journal of Virology↗

Evolution of puma lentivirus in bobcats ( Lynx rufus ) and mountain lions ( Puma concolor ) in North America

Mountain lions ( Puma concolor ) throughout North and South America are infected with puma lentivirus clade B (PLVB). A second, highly divergent lentiviral clade, PLVA, infects mountain lions in southern California and Florida. Bobcats ( Lynx rufus ) in these two geographic regions are also infected with PLVA, and to date, this is the only strain of lentivirus identified in bobcats. We sequenced full-length PLV genomes in order to characterize the molecular evolution of PLV in bobcats and mountain lions. Low sequence homology (88% average pairwise identity) and frequent recombination (1 recombination breakpoint per 3 isolates analyzed) were observed in both clades. Viral proteins have markedly different patterns of evolution; sequence homology and negative selection were highest in Gag and Pol and lowest in Vif and Env. A total of 1.7% of sites across the PLV genome evolve under positive selection, indicating that host-imposed selection pressure is an important force shaping PLV evolution. PLVA strains are highly spatially structured, reflecting the population dynamics of their primary host, the bobcat. In contrast, the phylogeography of PLVB reflects the highly mobile mountain lion, with diverse PLVB isolates cocirculating in some areas and genetically related viruses being present in populations separated by thousands of kilometers. We conclude that PLVA and PLVB are two different viral species with distinct feline hosts and evolutionary histories.

North America↗

In Vivo fitness associated with high virulence in a vertebrate virus is a complex trait regulated by host entry, replication, and shedding

The relationship between pathogen fitness and virulence is typically examined by quantifying only one or two pathogen fitness traits. More specifically, it is regularly assumed that within-host replication, as a precursor to transmission, is the driving force behind virulence. In reality, many traits contribute to pathogen fitness, and each trait could drive the evolution of virulence in different ways. Here, we independently quantified four viral infection cycle traits, namely, host entry, within-host replication, within-host coinfection fitness, and shedding, in vivo , in the vertebrate virus Infectious hematopoietic necrosis virus (IHNV). We examined how each of these stages of the viral infection cycle contributes to the fitness of IHNV genotypes that differ in virulence in rainbow trout. This enabled us to determine how infection cycle fitness traits are independently associated with virulence. We found that viral fitness was independently regulated by each of the traits examined, with the largest impact on fitness being provided by within-host replication. Furthermore, the more virulent of the two genotypes of IHNV we used had advantages in all of the traits quantified. Our results are thus congruent with the assumption that virulence and within-host replication are correlated but suggest that infection cycle fitness is complex and that replication is not the only trait associated with virulence.

Journal of Virology↗

Feline immunodeficiency virus cross-species transmission: Implications for emergence of new lentiviral infections

Owing to a complex history of host-parasite coevolution, lentiviruses exhibit a high degree of species specificity. Given the well-documented viral archeology of HIV emergence following human exposures to SIV, understanding processes that promote successful cross-species lentiviral transmissions is highly relevant. We have previously reported natural cross-species transmission of a subtype of feline immunodeficiency virus, puma lentivirus A (PLVA), between bobcats ( Lynx rufus ) and mountain lions ( Puma concolor ) in a small number of animals in California and Florida. In this study we investigate host-specific selection pressures, within-host viral fitness, and inter- vs. intra-species transmission patterns among a larger collection of PLV isolates from free-ranging bobcats and mountain lions. Analysis of proviral and viral RNA levels demonstrates that PLVA fitness is severely restricted in mountain lions compared to bobcats. We document evidence of diversifying selection in three of six PLVA genomes from mountain lions, but did not detect selection among twenty PLVA isolates from bobcats. These findings support that PLVA is a bobcat-adapted virus, which is less fit in mountain lions and under intense selection pressure in the novel host. Ancestral reconstruction of transmission events reveals intraspecific PLVA transmission has occurred among panthers ( Puma concolor coryi ) in Florida following initial cross-species infection from bobcats. In contrast, interspecific transmission from bobcats to mountain lions predominates in California. These findings document outcomes of cross-species lentiviral transmission events among felids that compare to emergence of HIV from nonhuman primates. IMPORTANCE Cross-species transmission episodes can be singular, dead-end events or can result in viral replication and spread in the new species. The factors that determine which outcome will occur are complex, and the risk of new virus emergence is therefore difficult to predict. Here we use molecular techniques to evaluate transmission, fitness, and adaptation of puma lentivirus A (PLVA) between bobcats and mountain lions in two geographic regions. Our findings illustrate that mountain lion exposure to PLVA is relatively common, but does not routinely result in infections communicable in the new host. This is attributed to efficient species barriers that largely prevent lentiviral adaptation. However, the evolutionary capacity for lentiviruses to adapt to novel environments may ultimately overcome host restriction mechanisms over time and under certain ecological circumstances. This phenomenon provides a unique opportunity to examine cross-species transmission events leading to new lentiviral emergence.

California, Florida↗

Neutralization-resistant variants of infectious hematopoietic necrosis virus have altered virulence and tissue tropism

Infectious hematopoietic necrosis virus (IHNV) is a rhabdovirus that causes an acute disease in salmon and trout. In this study, a correlation between changes in tissue tropism and specific changes in the virus genome appeared to be made by examining four IHNV neutralization-resistant variants (RB-1, RB-2, RB-3, and RB-4) that had been selected with the glycoprotein (G)-specific monoclonal antibody RB/B5. These variants were compared with the parental strain (RB-76) for their virulence and pathogenicity in rainbow trout after waterborne challenge. Variants RB-2, RB-3, and RB-4 were only slightly attenuated and showed distributions of viral antigen in the livers and hematopoietic tissues of infected fish similar to those of the parental strain. Variant RB-1, however, was highly attenuated and the tissue distribution of viral antigen in RB-1-infected fish was markedly different, with more viral antigen in brain tissue. The sequences of the G genes of all four variants and RB-76 were determined. No significant changes were found for the slightly attenuated variants, but RB-1 G had two changes at amino acids 78 and 218 that dramatically altered its predicted secondary structure. These changes are thought to be responsible for the altered tissue tropism of the virus. Thus, IHNV G, like that of rabies virus and vesicular stomatitis virus, plays an integral part in the pathogenesis of viral infection.

Journal of Virology↗

First juvenile Chum Salmon confirms successful reproduction for Pacific salmon in the North American Arctic

The distributional extent of Pacific salmon Oncorhynchus spp. in the North American Arctic is unresolved. While adult Pacific salmon have a recurring presence across the Alaskan North Slope and into the Canadian Arctic, it is uncertain if these fish are part of established Arctic populations, vagrants from outside sources reproducing unsuccessfully, or both. Here we present the first confirmed record of a juvenile Chum Salmon O. keta captured in the nearshore marine ecosystem in the North American Arctic. This provides the first scientific evidence of successful spawning and early marine survival of Pacific salmon in the North American Arctic. It was caught near Kaktovik, Alaska in August 2017 with a group of similarly sized age-0 Mackenzie River Arctic Cisco Coregonus autumnalis . Stable isotope and otolith microchemistry analyses are consistent with use of the nearshore estuarine corridor from the Mackenzie River west along the northern coast. This contributes critical information needed to identify, manage, and conserve biodiversity at the northern range edge, and will help to clarify the status of Pacific salmon as potentially emerging fisheries develop in the North American Arctic due to climate warming.

Alaska↗

Characterization and application of a quantitative DNA marker that discriminates sex in chinook salmon (Oncorhynchus tshawytscha)

A qualitative male-specific DNA marker (OT-24) was amplified by spPCR (single-primer polymerase chain reaction) from chinook salmon (Oncorhynchus tshawytscha) DNA along with several non-sex-linked products. The termini of the male-specific product were sequenced, and a pair of PeR primers were constructed for marker-specific PCR amplification. Dual primer PCR (dpPCR), with the marker-specific primers, amplified a product from both nudes and females. The amount of dpPCR product amplified from males was at least 100-fold greater than that from females. The quantitative difference between males and females was consistent among geographically distinct populations from western U.S. rivers. In addition, DNA sequence analysis indicated that OT-24 was highly conserved among geographically distinct salmon populations. The qualitative spPCR product segregated through several genetic crosses indicating equal sex ratios among progeny. Identification of the male and female juveniles by dpPCR was consistent with the spPCR analysis. There was no tissue specificity observed by spPCR or dpPCR analysis of this marker. A rapid DNA extraction method and dpPCR analysis were used to nonlethally determine sex ratios in wild spring chinook salmon adults, withheld for genetic and behavioral studies, prior to their development of gross sexual differences in their external morphology.

Canadian Journal of Fisheries and Aquatic Sciences↗

Sperm-cell ultrastructure of North American sturgeons. IV. The pallid sturgeon (Scaphirhynchus albus Forbes and Richardson, 1905)

Sperm-cell morphology and ultrastructure in the pallid sturgeon (Scaphirhynchus albus) were examined using transmission and scanning electron microscopy. Metrics and structure were compared with similar metrics obtained from other published descriptions of sturgeon sperm cells. General morphology was found to be similar to that of sperm cells of the white (Acipenser transmontanus), lake (A. fulvescens), stellate (A. stellatus), Chinese (A. sinensis), Russian (A. gueldenstaedti colchicus), and shortnose (A. brevirostrum) sturgeons, which all shared a gradual tapering of the nuclear diameter from posterior to anterior, unlike that of the Atlantic sturgeon (A. oxyrhynchus). The sperm cell of the pallid sturgeon was similar in size to that of the Atlantic sturgeon, being only slightly larger. The sperm cell of the pallid sturgeon differed from those of other sturgeons chiefly in the acrosomal region, where the posterolateral projections (PLP) have the shape of an acute triangle and are arranged in a spiral about the longitudinal axis of the cell. The PLP were longer than those of other sturgeons, being twice the length of those of the Atlantic sturgeon and 58% longer than those of the lake sturgeon. Also, in cross section the acrosome had the shape of a hollow cone rather than the cap of an oak tree acorn, as was found in ultrastructural studies of other sturgeons. In addition, we were able to confirm that the structural arrangement of the distal centriole of the midpiece is identical with that of the proximal centriole: nine sets of microtubular triplets around the periphery of the centriole. This information is of potential use to fishery biologists, forensic biologists, zoologists, reproductive physiologists, taxonomists, evolutionary biologists, and aquaculturists.

Canadian Journal of Zoology↗

Global analyses of brachiopod faunas through the Ordovician and Silurian transition: Reducing the role of the Lazarus effect

Global analyses of 88 families and 284 genera of brachiopods from middle Ashgill, Late Ordovician, to early-middle Rhuddanian, Early Silurian, indicate that 18.6% and 12.5% of families and 51.0% and 41.3% of genera were eliminated in the first and second phases of the end-Ordovician mass extinction, respectively, with the total loss of 28.4% of families and 69.0% of genera in the crisis. New investigation demonstrates that brachiopods, at both generic and familial levels, suffered greater during the first phase than during the second phase. Four groups (victims, relicts, survivors, and new arrivals) are distinguished by their stratigraphical ranges. Generic survivors, occurring in the Kosov Province during the Hirnantian, can be split into three types with respect to their changing abundance: increasing, declining, and Lazarus taxa. Among the 88 genera that survived, numerous declining genera occurred in the Hirnantian: 16 Lazarus families and 18 Lazarus genera are provisionally known and may be regarded as end members of the declining type. Comparison of the abundance, population size, and distribution patterns of declining and Lazarus taxa shows important similarities between these two types which contribute to a better understanding of the nature of Lazarus taxa. In addition to these biological attributes, taphonomic failure and generally poor preservation, together with collecting bias and inadequate systematic data, are clearly involved. More collections will undoubtedly globally reduce the number of Lazarus taxa. A single, common refugium for end-Ordovician brachiopods probably did not exist; rather, these taxa used paleogeographically scattered locations in a range of environments for survival. ?? 2006 NRC Canada.

Canadian Journal of Earth Sciences↗

A heat-flow reconnaissance of southeastern Alaska

Heat flow was measured at nine sites in crystalline and sedimentary rocks of southeastern Alaska. Seven of the sites, located between 115 and 155 km landward of the Queen Charlotte – Fairweather transform fault, have an average heat flow of 59 ± 6 mW m −2 . This value is significantly higher than the mean of 42 mW m −2 in the coastal provinces between Cape Mendocino and the Queen Charlotte Islands, to the south, and is lower than the mean of 72 ± 2 mW m −2 for 81 values within 100 km of the San Andreas transform fault, even farther south. This intermediate value suggests the absence of significant heat sinks associated with Cenozoic subduction and of heat sources related to either late Cenozoic tectono-magmatic events or significant shear-strain heating. At Warm Springs Bay, 75 km from the plate boundary, an anomalously high heat flow of 150 mW m −2 can most plausibly be ascribed to the thermal spring activity from which its name is derived. At Quartz Hill, 240 km landward of the plate boundary, a value of 115 mW m −2 might indicate a transition to a province of high heat flow resulting from late Tertiary and Quaternary extension and volcanism.

Alaska↗

Factors influencing coastal cutthroat trout (Oncorhynchus clarkii clarkii) seasonal survival rates: A spatially continuous approach within stream networks

Mark-recapture methods were used to examine watershed-scale survival of coastal cutthroat trout (Oncorhynchus clarkii clarkii) from two headwater stream networks. A total of 1725 individuals (???100 mm, fork length) were individually marked and monitored seasonally over a 3-year period. Differences in survival were compared among spatial (stream segment, subwatershed, and watershed) and temporal (season and year) analytical scales, and the effects of abiotic (discharge, temperature, and cover) and biotic (length, growth, condition, density, movement, and relative fish abundance) factors were evaluated. Seasonal survival was consistently lowest and least variable (years combined) during autumn (16 September - 15 December), and evidence suggested that survival was negatively associated with periods of low stream discharge. In addition, relatively low (-) and high (+) water temperatures, fish length (-), and boulder cover (+) were weakly associated with survival. Seasonal abiotic conditions affected the adult cutthroat trout population in these watersheds, and low-discharge periods (e.g., autumn) were annual survival bottlenecks. Results emphasize the importance of watershed-scale processes to the understanding of population-level survival.

Canadian Journal of Fisheries and Aquatic Sciences↗

Effect of feeding and of DDT on the activity of hepatic glucose 6- phosphate dehydrogenase in two salmonids

The specific activity of liver glucose 6-phosphate dehydrogenase in yearling rainbow trout remained unchanged when the fish were starved for periods as long as 8 weeks and when starved animals were fed diets of various compositions. Injection of insulin concurrently with refeeding also failed to alter the specific activity of the enzyme in trout. The absence of a dietary or insulin influence on the teleost enzyme system is to be contrasted with studies in mammals in which the activity of hepatic glucose 6-P dehydrogenase was markedly stimulated after refeeding starved animals or injection of insulin.Ingestion of the pesticide DDT by juvenile coho salmon or adult rainbow trout also had no effect on the specific activity of liver glucose 6-P dehydrogenase and DDT failed to inhibit the rainbow trout enzyme in vitro. These results also differ considerably from those found in higher animals.These results suggest that the glucose 6-P dehydrogenase enzyme in teleosts may be under a different type of regulatory control from that found in mammals.

Journal of the Fisheries Research Board of Canada↗

Immersion vaccination of sockeye salmon (Oncorhynchus nerka) with two pathogenic strains of Vibrio anguillarum

Sockeye salmon ( Oncorhynchus nerka ) were immersion-vaccinated in suspensions containing 5&ensp;&times;&ensp;10 7 , 5&ensp;&times;&ensp;10 6 , 5&ensp;&times;&ensp;10 5 , or 5&ensp;&times;&ensp;10 4 bacteria/mL of bivalent or monovalent, formalin-killed Vibrio anguillarum , Types I and II. The fish were split into two lots and held for 54&ensp;d. At that time one lot was challenged with living, virulent V . anguillarum , Type I, and one with living, virulent V . anguillarum , Type II. Immunization with bivalent bacterin effectively protected the fish from vibriosis, but monovalent vaccine was effective only against the homologous challenge. Immunization with the highest concentration of Type I monovalent bacterin resulted in 0% Type I and 58% Type II challenge mortality. Immunization with the highest concentration of Type II monovalent bacterin resulted in 41% Type I and 0% Type II challenge mortality. Immunization with the highest concentration of bivalent Type I/Type II bacterin resulted in 2% mortality in both challenges. Protective bacterins were effective at concentrations down to 5&ensp;&times;&ensp;10 5 bacteria/mL. Key words : immersion vaccination, bivalent vaccines, Vibrio anguillarum , vibriosis.

Journal of the Fisheries Research Board of Canada↗