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Diet-related die-off of captive black-crowned night herons

Several species of herons, which are top-level consumers in aquatic food chains, have experienced population declines in certain areas o f their normal range (7,13) -- areas in which elevated levels of various environmental pollutants are known to occur. (6) To determine the effects of environmental contaminants on the Ardeidae, a colony of black-crowned night herons (Nycticorax nycticorax) was established in 1972 at the Patuxent Wildlife Research Center. The night heron was selected as the model species because of its widespread occurrence and its ability to survive and reproduce in captivity. Birds for the colony were obtained from either the New York Zoological Park and Dallas Zoo or were wild-caught along the Maryland and Virginia coasts in 1972, 1973, and 1975. This report describes a die-off in the colony following a change in the origina of their food source. The data suggest that the mortality was diet-related, most likely caused by vitamin E deficiency. Excessive dietary thiaminase may have resulted in concurrent thiamine deficiency, but evidence for this is equivocal.

American Association of Zoo Veterinarians, Annual ↗

Empirical comparison of uniform and non-uniform probability sampling for estimating numbers of red-cockaded woodpecker colonies

Four sampling and estimation methods for estimating the number of red-cockaded woodpecker colonies on National Forests in the Southeast were compared, using samples chosen from simulated populations based on the observed sample. The methods included (1) simple random sampling without replacement using a mean per sampling unit estimator, (2) simple random sampling without replacement with a ratio per pine area estimator, (3) probability proportional to 'size' sampling with replacement, and (4) probability proportional to 'size' without replacement using Murthy's estimator. The survey sample of 274 National Forest compartments (1000 acres each) constituted a superpopulation from which simulated stratum populations were selected with probability inversely proportional to the original probability of selection. Compartments were originally sampled with probabilities proportional to the probabilities that the compartments contained woodpeckers ('size'). These probabilities were estimated with a discriminant analysis based on tree species and tree age. The ratio estimator would have been the best estimator for this survey based on the mean square error. However, if more accurate predictions of woodpecker presence had been available, Murthy's estimator would have been the best. A subroutine to calculate Murthy's estimates is included; it is computationally feasible to analyze up to 10 samples per stratum.

American Statistical Association, Proceedings of t↗

PCBs, organochlorine pesticides, and reproduction in river otters from Louisiana

Reproductive tracts from 89 3-year-old female river otters (Lutra canadensis), from Louisiana were examined. Eighteen of these were in a reproductive phase out of synchrony with the expected population norms. Eight of 32 otters had fewer embryos than corpora lutea, indicating intrauterine mortality in 25% of the sample. Chemical analyses of liver tissue from 57 otters revealed a low prevalence of polychlorinated biphenyls (PCB) and organochlorine pesticide contamination. These low Ievels of organochlorine compounds were not associated with atypical reproductive synchrony or intrauterine mortality.

Proceedings of the Annual Conference of the Southe↗

Rehabilitation of birds oiled on two mid-Atlantic estuaries

An estimated 52,500 birds died as a result of 7 major oil spills on 2 mid-Atlantic estuaries between 1973-78. Ruddy ducks ( Oxyura jamaicensis ) constituted 98% of 12,500 birds known to have died from 5 spills on the Delaware River. Seventy-six percent of 40,000 dead birds from 2 Chesapeake Bay spills were horned grebes ( Podiceps auritus ) and oldsquaw ( Clangula hyemalis ). Oiled waterfowl that were captured alive (6% of the estimated mortality) were cleaned with a variety of cleaning agents and techniques. High mortality occurred during and shortly after cleaning, and was apparently due to hypothermia and to toxicity of solvent cleaning agents. Eighty-two percent of the 3,113 birds that were cleaned died prior to or at time of release. The fate of the remaining 18% is unknown. Petroleum solvents used as cleaning agents were toxic to the birds. Most detergents left a surfactant (wetting agent) on the feathers which resulted in subsequent wetting of released birds. Although rehabilitation techniques have improved in recent years, high bird mortality can be expected following future oil spills.

Chesapeake Bay, Delaware River↗

Multivariate curve-fitting in GAUSS

Multivariate curve-fitting techniques for repeated measures have been developed and an interactive program has been written in GAUSS. The program implements not only the one-factor design described in Morrison (1967) but also includes pairwise comparisons of curves and rates, a two-factor design, and other options. Strategies for selecting the appropriate degree for the polynomial are provided. The methods and program are illustrated with data from studies of the effects of environmental contaminants on ducklings, nesting kestrels and quail.

American Statistical Association, Proceedings of t↗