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Simulated fissioning of uranium and testing of the fission-track dating method

A computer program (FTD-SIM) faithfully simulates the fissioning of 238U with time and 235U with neutron dose. The simulation is based on first principles of physics where the fissioning of 238U with the flux of time is described by Ns = ??f 238Ut and the fissioning of 235U with the fluence of neutrons is described by Ni = ??235U??. The Poisson law is used to set the stochastic variation of fissioning within the uranium population. The life history of a given crystal can thus be traced under an infinite variety of age and irradiation conditions. A single dating attempt or up to 500 dating attempts on a given crystal population can be simulated by specifying the age of the crystal population, the size and variation in the areas to be counted, the amount and distribution of uranium, the neutron dose to be used and its variation, and the desired ratio of 238U to 235U. A variety of probability distributions can be applied to uranium and counting-area. The Price and Walker age equation is used to estimate age. The output of FTD-SIM includes the tabulated results of each individual dating attempt (sample) on demand and/or the summary statistics and histograms for multiple dating attempts (samples) including the sampling age. An analysis of the results from FTD-SIM shows that: (1) The external detector method is intrinsically more precise than the population method. (2) For the external detector method a correlation between spontaneous track count, Ns, and induced track count, Ni, results when the population of grains has a stochastic uranium content and/or when the counting areas between grains are stochastic. For the population method no correlation can exist. (3) In the external detector method the sampling distribution of age is independent of the number of grains counted. In the population method the sampling distribution of age is highly dependent on the number of grains counted. (4) Grains with zero-track counts, either in Ns or Ni, are in integral part of fissioning theory and under certain circumstances must be included in any estimate of age. (5) In estimating standard error of age the standard error of Ns and Ni and ?? must be accurately estimated and propagated through the age equation. Several statistical models are presently available to do so. ?? 1985.

Nuclear Tracks and Radiation Measurements (1982)↗

Latitudinal variation in avian incubation attentiveness and a test of the food limitation hypothesis

Avian incubation attentiveness has important fitness consequences through its influence on the number and quality of hatched young and energetic costs imposed on parents. Nest attentiveness is highly variable across species and geographical regions. We reviewed the literature and found a worldwide pattern that nest attentiveness of passerines is generally lower in south temperate and tropical regions than in north temperate regions. We also conducted a food manipulation experiment to assess the extent to which nest attentiveness may reflect proximate responses versus an evolved behaviour. We used the karoo prinia, Prinia maculosa, in South Africa, which has very low nest attentiveness (???49%) compared with that of many passerine birds. We provided supplemental food during early incubation to experimental females and compared nest attentiveness and on- and off-bout lengths of experimental and paired control females.??Nest attentiveness of females at food-provisioned nests was significantly higher than that of control females (57% versus 49%). Food-supplemented females also spent significantly less time off the nest than did control females, whereas mean on-bout lengths did not differ. However, mean nest attentiveness of food-provisioned females was still substantially below that of other similar bird species worldwide. Food can be an important proximate influence on parental care behaviour, but proximate influences of food do not explain broad latitudinal patterns of attentiveness. ?? 2007 The Association for the Study of Animal Behaviour.

Animal Behaviour↗

Cutthroat trout virus as a surrogate in vitro infection model for testing inhibitors of hepatitis E virus replication

Hepatitis E virus (HEV) is one of the most important causes of acute hepatitis worldwide. Although most infections are self-limiting, mortality is particularly high in pregnant women. Chronic infections can occur in transplant and other immune-compromised patients. Successful treatment of chronic hepatitis E has been reported with ribavirin and pegylated interferon-alpha, however severe side effects were observed. We employed the cutthroat trout virus (CTV), a non-pathogenic fish virus with remarkable similarities to HEV, as a potential surrogate for HEV and established an antiviral assay against this virus using the Chinook salmon embryo (CHSE-214) cell line. Ribavirin and the respective trout interferon were found to efficiently inhibit CTV replication. Other known broad-spectrum inhibitors of RNA virus replication such as the nucleoside analog 2′-C-methylcytidine resulted only in a moderate antiviral activity. In its natural fish host, CTV levels largely fluctuate during the reproductive cycle with the virus detected mainly during spawning. We wondered whether this aspect of CTV infection may serve as a surrogate model for the peculiar pathogenesis of HEV in pregnant women. To that end the effect of three sex steroids on in vitro CTV replication was evaluated. Whereas progesterone resulted in marked inhibition of virus replication, testosterone and 17β-estradiol stimulated viral growth. Our data thus indicate that CTV may serve as a surrogate model for HEV, both for antiviral experiments and studies on the replication biology of the Hepeviridae.

Antiviral Research↗

Potential environmental issues of CO 2 storage in deep saline aquifers: Geochemical results from the Frio-I Brine Pilot test, Texas, USA

Sedimentary basins in general, and deep saline aquifers in particular, are being investigated as possible repositories for large volumes of anthropogenic CO 2 that must be sequestered to mitigate global warming and related climate changes. To investigate the potential for the long-term storage of CO 2 in such aquifers, 1600 t of CO 2 were injected at 1500 m depth into a 24-m-thick "C" sandstone unit of the Frio Formation, a regional aquifer in the US Gulf Coast. Fluid samples obtained before CO 2 injection from the injection well and an observation well 30 m updip showed a Na–Ca–Cl type brine with ∼93,000 mg/L TDS at saturation with CH 4 at reservoir conditions; gas analyses showed that CH 4 comprised ∼95% of dissolved gas, but CO 2 was low at 0.3%. Following CO 2 breakthrough, 51 h after injection, samples showed sharp drops in pH (6.5–5.7), pronounced increases in alkalinity (100–3000 mg/L as HCO 3 ) and in Fe (30–1100 mg/L), a slug of very high DOC values, and significant shifts in the isotopic compositions of H 2 O, DIC, and CH 4 . These data, coupled with geochemical modeling, indicate corrosion of pipe and well casing as well as rapid dissolution of minerals, especially calcite and iron oxyhydroxides, both caused by lowered pH (initially ∼3.0 at subsurface conditions) of the brine in contact with supercritical CO 2 . These geochemical parameters, together with perfluorocarbon tracer gases (PFTs), were used to monitor migration of the injected CO 2 into the overlying Frio “B”, composed of a 4-m-thick sandstone and separated from the “C” by ∼15 m of shale and siltstone beds. Results obtained from the Frio “B” 6 months after injection gave chemical and isotopic markers that show significant CO 2 (2.9% compared with 0.3% CO 2 in dissolved gas) migration into the “B” sandstone. Results of samples collected 15 months after injection, however, are ambiguous, and can be interpreted to show no additional injected CO 2 in the “B” sandstone. The presence of injected CO 2 may indicate migration from “C” to “B” through the intervening beds or, more likely, a short-term leakage through the remedial cement around the casing of a 50-year old well. Results obtained to date from four shallow monitoring groundwater wells show no brine or CO 2 leakage through the Anahuac Formation, the regional cap rock.

Texas↗

Testing the interactive effects of flooding and salinity on tidal marsh plant productivity

Tidal wetlands support plant communities that facilitate carbon storage, accrete soil, and provide habitat for terrestrial and aquatic species. Climate change is likely to alter estuaries through sea-level rise and changing precipitation patterns, although the ecological responses are uncertain. We were interested in plant responses to physiological stress induced by elevated water salinity and flooding conditions, which may be more prevalent under climate change. . We used a greenhouse experiment and factorial flooding (1, 12, 24, and 48 % time) and salinity (0, 5, 15, 30 PSU) treatments to evaluate the productivity responses of three emergent herbaceous species ( Carex lyngbyei, Triglochin maritima, and Argentina pacifica ) common to tidal marshes of the Pacific Northwest, USA. We measured weekly changes in plant height and final above and belowground biomass for all species after 10 weeks. Increased salinity reduced final above and belowground biomass significantly in all three species, with A. pacifica responding the most, followed by C. lyngbyei and T. maritima . Increased flooding also reduced total biomass in A. pacifica and T. maritima . There was a significant response in C. lyngbyei aboveground biomass and A. pacifica height to the flooding-salinity interaction. These results indicate emergent plant community composition may change in response to novel climate conditions in estuaries, driven by distinct physiological tolerances to salinity and flooding, and highlight the importance of considering multiple climate drivers when projecting ecosystem change. This may be especially true for estuaries that currently have prolonged freshwater phases like those in the Pacific Northwest.

Aquatic Botany↗

Energy development and avian nest survival in Wyoming, USA: A test of a common disturbance index

Global energy demands continue to result in new and emerging sources of anthropogenic disturbance to populations and systems. Here, we assessed the influence of natural gas development on a critical component of fitness (nest survival) for Brewer’s sparrow ( Spizella breweri ), sagebrush sparrow ( Artemisiospiza nevadensis ), and sage thrasher ( Oreoscoptes montanus ), three species of sagebrush-obligate songbirds that are of conservation concern, and assessed the efficacy of a commonly used index of oil and gas development intensity (well density) for estimating habitat transformation and predicting species’ responses. During 2008–2009 and 2011–2012 we monitored 926 nests within two natural gas fields in western Wyoming, USA. We calculated landscape metrics (habitat loss, amount of edge, patch shape complexity, and mean patch size) to identify the aspect of landscape transformation most captured by well density. Well density was most positively associated with the amount of sagebrush habitat loss within 1 square kilometer. Nest survival was relatively invariant with respect to well density for all three species. In contrast, nest survival rates of all three species generally decreased with surrounding habitat loss due to energy development. Thus, although well density and habitat loss were strongly correlated, well density resulted in overly conservative estimates of nest survival probability. Our results emphasize the importance of careful evaluation of the appropriateness of particular indices for quantifying the effects of human-induced habitat change. For managers concerned about the effects of natural gas development or similar forms of human land use to co-occurring breeding birds, we recommend minimizing the amount of associated habitat conversion.

Wyoming↗

A test of the cross-scale resilience model: Functional richness in Mediterranean-climate ecosystems

Ecological resilience has been proposed to be generated, in part, in the discontinuous structure of complex systems. Environmental discontinuities are reflected in discontinuous, aggregated animal body mass distributions. Diversity of functional groups within body mass aggregations (scales) and redundancy of functional groups across body mass aggregations (scales) has been proposed to increase resilience. We evaluate that proposition by analyzing mammalian and avian communities of Mediterranean-climate ecosystems. We first determined that body mass distributions for each animal community were discontinuous. We then calculated the variance in richness of function across aggregations in each community, and compared observed values with distributions created by 1000 simulations using a null of random distribution of function, with the same n, number of discontinuities and number of functional groups as the observed data. Variance in the richness of functional groups across scales was significantly lower in real communities than in simulations in eight of nine sites. The distribution of function across body mass aggregations in the animal communities we analyzed was non-random, and supports the contentions of the cross-scale resilience model. ?? 2007 Elsevier B.V. All rights reserved.

Ecological Complexity↗

Test of a foraging-bioenergetics model to evaluate growth dynamics of endangered pallid sturgeon ( Scaphirhynchus albus )

Factors affecting feeding and growth of early life stages of the federally endangered pallid sturgeon ( Scaphirhynchus albus ) are not fully understood, owing to their scarcity in the wild. In this study was we evaluated the performance of a combined foraging-bioenergetics model as a tool for assessing growth of age-0 pallid sturgeon in the Missouri River. In the laboratory, three size classes of sturgeon larvae (18–44 mm; 0.027–0.329 g) were grown for 7 to 14 days under differing temperature (14–24 °C) and prey density (0–9 Chironomidae larvae/d) regimes. After accounting for effects of water temperature and prey density on fish activity, we compared observed final weight, final length, and number of prey consumed to values generated from the foraging-bioenergetics model. When confronted with an independent dataset, the combined model provided reliable estimates (within 13% of observations) of fish growth and prey consumption, underscoring the usefulness of the modeling approach for evaluating growth dynamics of larval fish when empirical data are lacking.

Ecological Modelling↗

Testing ecosystem accounting in the United States: A case study for the Southeast

Ecosystem accounts, as formalized by the System of Environmental-Economic Accounting Experimental Ecosystem Accounts (SEEA EEA), have been compiled in a number of countries, yet there have been few attempts to develop them for the U.S. We explore the potential for U.S. ecosystem accounting by compiling ecosystem extent, condition, and ecosystem services supply and use accounts for a ten-state region in the Southeast. The pilot accounts address air quality, water quality, biodiversity, carbon storage, recreation, and pollination for selected years from 2001 to 2015. Results illustrate how information from ecosystem accounts can contribute to policy and decision-making. Using an example from Atlanta, we also show how ecosystem accounts can be considered alongside other SEEA accounts to give a more complete picture of a local area’s environmental-economic trends. The process by which we determined where to place metrics within the accounting framework, which was strongly informed by the National Ecosystem Services Classification System (NESCS), can provide guidance for future ecosystem accounts in the U.S. and other countries. Finally, we identify knowledge gaps that limit the inclusion of certain ecosystem services in the accounts and suggest future research that can close these gaps and improve future U.S. ecosystem accounts.

Missouri, Arkansas, Louisiana, Tennessee, Mississi↗

Improving the ecological relevance of toxicity tests on scleractinian corals: Influence of season, life stage, and seawater temperature

Metal pollutants in marine systems are broadly acknowledged as deleterious: however, very little data exist for tropical scleractinian corals. We address this gap by investigating how life-history stage, season and thermal stress influence the toxicity of copper (Cu) and lead (Pb) in the coral Pocillopora damicornis . Our results show that under ambient temperature, adults and larvae appear to tolerate exposure to unusually high levels of copper (96 h-LC 50 ranging from 167 to 251 μg Cu L −1 ) and lead (from 477 to 742 μg Pb L −1 ). Our work also highlights that warmer conditions (seasonal and experimentally manipulated) reduce the tolerance of adults and larvae to Cu toxicity. Despite a similar trend observed for the response of larvae to Pb toxicity to experimentally induced increase in temperature, surprisingly adults were more resistant in warmer condition to Pb toxicity. In the summer adults were less resistant to Cu toxicity (96 h-LC 50 = 175 μg L −1 ) than in the winter (251 μg L −1 ). An opposite trend was observed for the Pb toxicity on adults between summer and winter (96 h-LC 50 of 742 vs 471 μg L −1 , respectively). Larvae displayed a slightly higher sensitivity to Cu and Pb than adults. An experimentally induced 3 °C increase in temperature above ambient decreased larval resistance to Cu and Pb toxicity by 23–30% (96 h-LC 50 of 167 vs 129 μg Cu L −1 and 681 vs 462 μg Pb L −1 ). Our data support the paradigm that upward excursions in temperature influence physiological processes in corals that play key roles in regulating metal toxicity. These influences are more pronounced in larva versus adult corals. These findings are important when contextualized climate change-driven warming in the oceans and highlight that predictions of ecological outcomes to metal pollutants will be improved by considering environmental context and the life stages of organism under study.

Environmental Pollution↗

Testing for the ‘predictability’ of dynamically triggered earthquakes in Geysers Geothermal Field

The Geysers geothermal field is well known for being susceptible to dynamic triggering of earthquakes by large distant earthquakes, owing to the introduction of fluids for energy production. Yet, it is unknown if dynamic triggering of earthquakes is ‘predictable’ or whether dynamic triggering could lead to a potential hazard for energy production. In this paper, our goal is to investigate the characteristics of triggering and the physical conditions that promote triggering to determine whether or not triggering is in anyway foreseeable. We find that, at present, triggering in The Geysers is not easily ‘predictable’ in terms of when and where based on observable physical conditions. However, triggered earthquake magnitude positively correlates with peak imparted dynamic stress, and larger dynamic stresses tend to trigger sequences similar to mainshock–aftershock sequences. Thus, we may be able to ‘predict’ what size earthquakes to expect at The Geysers following a large distant earthquake.

California↗

Testing the effect of habitat structure and complexity on nekton assemblages using experimental oyster reefs

Structurally complex habitats are often associated with more diverse and abundant species assemblages in both aquatic and terrestrial ecosystems. Biogenic reefs formed by the eastern oyster ( Crassostrea virginica ) are complex in nature and are recognized for their potential habitat value in estuarine systems along the US Atlantic and Gulf of Mexico coasts. Few studies, however, have examined the response of nekton to structural complexity within oyster reefs. We used a quantitative sampling technique to examine how the presence and complexity of experimental oyster reefs influence the abundance, biomass, and distribution of nekton by sampling reefs 4 months and 16 months post-construction. Experimental oyster reefs were colonized immediately by resident fishes and decapod crustaceans, and reefs supported a distinct nekton assemblage compared to mud-bottom habitat. Neither increased reef complexity, nor age of the experimental reef resulted in further changes in nekton assemblages or increases in nekton abundance or diversity. The presence of oyster reefs per se was the most important factor determining nekton usage.

Journal of Experimental Marine Biology and Ecology↗

Testing coral-based tropical cyclone reconstructions: An example from Puerto Rico

Complimenting modern records of tropical cyclone activity with longer historical and paleoclimatological records would increase our understanding of natural tropical cyclone variability on decadal to centennial time scales. Tropical cyclones produce large amounts of precipitation with significantly lower δ 18 O values than normal precipitation, and hence may be geochemically identifiable as negative δ 18 O anomalies in marine carbonate δ 18 O records. This study investigates the usefulness of coral skeletal δ 18 O as a means of reconstructing past tropical cyclone events. Isotopic modeling of rainfall mixing with seawater shows that detecting an isotopic signal from a tropical cyclone in a coral requires a salinity of ~ 33 psu at the time of coral growth, but this threshold is dependent on the isotopic composition of both fresh and saline end-members. A comparison between coral δ 18 O and historical records of tropical cyclone activity, river discharge, and precipitation from multiple sites in Puerto Rico shows that tropical cyclones are not distinguishable in the coral record from normal rainfall using this approach at these sites.

Puerto Rico↗

Testing the use of bulk organic δ 13 C, δ 15 N, and C org :N tot ratios to estimate subsidence during the 1964 great Alaska earthquake

During the M w 9.2 1964 great Alaska earthquake, Turnagain Arm near Girdwood, Alaska subsided 1.7 ± 0.1 m based on pre- and postearthquake leveling. The coseismic subsidence in 1964 caused equivalent sudden relative sea-level (RSL) rise that is stratigraphically preserved as mud-over-peat contacts where intertidal silt buried peaty marsh surfaces. Changes in intertidal microfossil assemblages across these contacts have been used to estimate subsidence in 1964 by applying quantitative microfossil transfer functions to reconstruct corresponding RSL rise. Here, we review the use of organic stable C and N isotope values and C org :N tot ratios as alternative proxies for reconstructing coseismic RSL changes, and report independent estimates of subsidence in 1964 by using δ 13 C values from intertidal sediment to assess RSL change caused by the earthquake. We observe that surface sediment δ 13 C values systematically decrease by ∼4‰ over the ∼2.5 m increase in elevation along three 60- to 100-m-long transects extending from intertidal mud flat to upland environments. We use a straightforward linear regression to quantify the relationship between modern sediment δ 13 C values and elevation ( n = 84, R 2 = 0.56). The linear regression provides a slope–intercept equation used to reconstruct the paleoelevation of the site before and after the earthquake based on δ 13 C values in sandy silt above and herbaceous peat below the 1964 contact. The regression standard error (average = ±0.59‰) reflects the modern isotopic variability at sites of similar surface elevation, and is equivalent to an uncertainty of ±0.4 m elevation with respect to Mean Higher High Water. To reduce potential errors in paleoelevation and subsidence estimates, we analyzed multiple sediment δ 13 C values in nine cores on a shore-perpendicular transect at Bird Point. Our method estimates 1.3 ± 0.4 m of coseismic RSL rise across the 1964 contact by taking the arithmetic mean of the differences ( n = 9) between reconstructed elevations for sediment above and below the 1964 earthquake subsidence contact. This estimate compares well with independent subsidence estimates derived from post-earthquake leveling in Turnagain Arm, and from microfossil transfer functions at Girdwood (1.50 ± 0.32 m). While our results support the use of bulk organic δ 13 C for reconstructing RSL change in southern Alaska, the variability of stable isotope values in modern and buried intertidal sediment required the analysis of multiple samples to reduce error.

Alaska↗

Testing independent and interactive effects of corticosterone and synergized resmethrin on the immune response to West Nile virus in chickens

Public health agencies utilize aerial insecticides to interrupt an active West Nile virus (WNV) transmission cycle, which may expose WNV-infected birds to these agents. Although resmethrin has been considered benign to birds, no studies have evaluated whether the environmentally employed form of resmethrin with PBO synergist (synergized resmethrin (SR)) can suppress avian immunity to WNV infection and enhance a bird's host competence. Recognizing that wild birds confront toxicological stressors in the context of various physiological states, we exposed four groups ( n = 9–11) of 9-week-old chickens ( Gallus domesticus ) to drinking water with either SR (three alternate days at 50 μg/l resmethrin + 150 μg/l piperonyl butoxide), CORT (10 days at 20 mg/l to induce subacute stress), the combination of SR and CORT, or 0.10% ethanol vehicle coincident with WNV infection. Compared to controls, SR treatment did not magnify but extended viremia by 1 day, and depressed IgG; CORT treatment elevated (mean, 4.26 log 10 PFU/ml) and extended viremia by 2 days, enhanced IgM and IgG, and increased oral virus. The combination of SR and CORT increased the number of chickens that shed oral virus compared to those treated with CORT alone. None of the chickens developed a readily infectious viremia to mosquitoes (none ≥5 log 10 PFU/ml), but viremia in a CORT-exposed chicken was up to 4.95 log 10 PFU/ml. Given that SR is utilized during WNV outbreaks, continued work toward a complete risk assessment of the potential immunotoxic effects of SR is warranted. This would include parameterization of SR exposures with immunological consequences in wild birds using both replicating (in the laboratory) and non-replicating (in the field) antigens. As a start, this study indicates that SR can alter some immunological parameters, but with limited consequences to primary WNV infection outcome, and that elevated CORT mildly enhances SRs immunotoxicity in chickens.

Toxicology↗

Testing a small UAS for mapping artisanal diamond mining sites in Africa

Remote sensing technology is advancing at an unprecedented rate. At the forefront of the new technological developments are unmanned aircraft systems (UAS). The advent of small, lightweight, low-cost, and user-friendly UAS is greatly expanding the potential applications of remote sensing technology and improving the set of tools available to researchers seeking to map and monitor terrain from above. In this article, we explore the applications of a small UAS for mapping informal diamond mining sites in Africa. We found that this technology provides aerial imagery of unparalleled resolution in a data-sparse, difficult to access, and remote terrain.

Photogrammetric Engineering and Remote Sensing↗

Tests of peak flow scaling in simulated self-similar river networks

The effect of linear flow routing incorporating attenuation and network topology on peak flow scaling exponent is investigated for an instantaneously applied uniform runoff on simulated deterministic and random self-similar channel networks. The flow routing is modelled by a linear mass conservation equation for a discrete set of channel links connected in parallel and series, and having the same topology as the channel network. A quasi-analytical solution for the unit hydrograph is obtained in terms of recursion relations. The analysis of this solution shows that the peak flow has an asymptotically scaling dependence on the drainage area for deterministic Mandelbrot-Vicsek (MV) and Peano networks, as well as for a subclass of random self-similar channel networks. However, the scaling exponent is shown to be different from that predicted by the scaling properties of the maxima of the width functions. ?? 2001 Elsevier Science Ltd. All rights reserved.

Advances in Water Resources↗

Bark heat resistance of small trees in Californian mixed conifer forests: Testing some model assumptions

An essential component to models of fire-caused tree mortality is an assessment of cambial damage. Cambial heat resistance has been traditionally measured in large overstory trees with thick bark, although small trees have thinner bark and thus are more sensitive to fire. We undertook this study to determine if current models of bark heat transfer are applicable to small trees (<20 cm diameter at breast height (dbh)). We performed this work in situ on four common species in the mixed conifer forests of the Sierra Nevada, California. The allometric relationship between bole diameter and bark thickness for each species was linear, even for very small trees (5 cm dbh). Heating experiments demonstrated that bark thickness was the primary determinant of cambial heat resistance. We found only slight, but statistically significant, among species differences in bark thermal properties. Our most significant finding was that small trees were more resistant to heating than expected from commonly used models of bark heat transfer. Our results may differ from those of existing models because we found smaller trees to have a greater proportion of inner bark, which appears to have superior insulating properties compared to outer bark. From a management perspective, growth projections suggest that a 50-year fire-free interval may allow some fire intolerant species to achieve at least some degree of cambial heat resistance in the Sierra Nevada.

Forest Ecology and Management↗