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Encroachment of oriental bittersweet into Pitcher’s thistle habitat

Common invasive species and rare endemic species can grow and interact at the ecotone between forested and non-forested dune habitats. To investigate these interactions, a comparison of the proximity and community associates of a sympatric invasive ( Celastrus orbiculatus ; oriental bittersweet) and native ( C. scandens ; American bittersweet) liana species to federally threatened Cirsium pitcheri (Pitcher's thistle) in the dunes habitats of Lake Michigan was conducted. Overall, the density of the invasive liana species was significantly greater in proximity to C. pitcheri than the native species. On the basis of composition, the three focal species occurred in both foredune and blowout habitats. The plant communities associated with the three focal species overlapped in ordination space, but there were significant differences in composition. The ability of C. orbiculatus to rapidly grow and change the ecological dynamics of invasion sites adds an additional threat to the successional habitats of C. pitcheri .

Lake Michigan↗

Monitoring and research at Walnut Creek National Wildlife Refuge

Walnut Creek National Wildlife Refuge-Prairie Learning Center (Walnut Creek or the Refuge) is one of the newest additions to the National Wildlife Refuge System, which consists of over 480 units throughout the United States operated by the U.S. Department of the Interior, Fish and Wildlife Service (the Service). Located about 20 miles east of Des Moines, Iowa, the Refuge has an approved acquisition boundary containing 8,654 acres (Figure 1). Acquisition is from willing sellers only, and to date the Service has purchased approximately 5,000 acres. The acquisition boundary encompasses about 43% of the watershed of Walnut Creek, which bisects the Refuge and drains into the Des Moines River to the southeast. Approximately 25%-30% of the Walnut Creek watershed is downstream of the Refuge. As authorized by Congress in 1990, the purposes of the Refuge are to (U.S. Fish and Wildlife Service 1992): • restore native tallgrass pairie, wetland, and woodland habitats for breeding and migratory waterfowl and resident wildlife; • serve as a major environmental education center providing opportunities for study; • provide outdoor recreation benefits to the public; and • provide assistance to local landowners to improve their lands for wildlife habitat. To implement these purposes authorized by Congress, the Refuge has established the goal of recreating as nearly as possible the natural communities that existed at the time of settlement by Euro-Americans (circa 1840). Current land use is largely agricultural, including 69% cropland, 17% grazed pasture, and 7.5% grassland (dominantly brome) enrolled in the Conservation Reserve Program). About 1,395 acres of relict native communities also exist on the Refuge, including prairie (725 acres), oak savanna and woodland (450 acres), and riparian or wetland areas (220 acres). Some of these relicts are highly restorable; others contain only a few prairie plants in a matrix of brome and will be more difficult to restore. When the process of restoration (management and enhancement of existing remnant communities) and reconstruction (reestablishment of native communities on agricultural sites) is completed, it is anticipated that the Refuge will consist of approximately 5,900 acres of prairie, 2,000 acres of oak savanna or woodland, and 750 acres of wetland and riparian communities. Ecological restoration and reconstruction at this scale in the tallgrass prairie ecosystem is unprecedented in the United States.

Report↗

Colocating artificial intelligence data centers with energy infrastructure on Federal public lands—A science synthesis and spatial analysis to inform decision making

Executive Summary Artificial intelligence (AI) is rapidly transforming industries and economies, creating an urgent need to strategically plan for the energy and infrastructure required to support increasing AI use. U.S. Federal agencies and bureaus have been directed to explore ways to accelerate permitting, development, and deployment of energy resources and AI technologies, including encouraging the colocation of energy infrastructure and data centers. To inform these initiatives, this report synthesizes relevant scientific information and presents a spatial analysis of existing energy infrastructure and data centers on or near U.S. Federal public lands managed by the Bureau of Land Management (BLM). The purpose of this science synthesis and spatial analysis is to provide the BLM with foundational information for considering potential colocation of data centers with energy infrastructure on Federal public lands to support evidence-based decisions. Additionally, this report provides insight into current (2025) and potential future energy demands by providing projections of a range of potential future environmental conditions relevant to maintaining industry-recommended cooling temperature standards necessary for efficient data center operations. As a part of this effort, a rapid response literature review was conducted of the best available science on the topic of data center development and energy infrastructure in July–August 2025, supplemented by additional resources recommended by U.S. Federal agency and bureau subject matter experts (hereafter experts; including the U.S. Department of Energy National Laboratory of the Rockies) and peer reviewers. To better understand current conditions relevant to AI data center development, a spatial analysis was conducted across Alaska and 11 States in the Western United States, Arizona, California, Colorado, Idaho, Montana, Nevada, New Mexico, Oregon, Utah, Washington, and Wyoming, all of which contain extensive BLM-managed surface lands (hereafter referred to as “BLM lands”) that could be considered for the colocation of energy infrastructure and AI data centers. This effort identified BLM lands within 10 miles of existing transmission lines, consistent with methods used in previous BLM programmatic environmental impact statements. This report describes the types of data centers operating within the United States, which vary in ownership, size, technology, and proximity to end users. This report then outlines the primary considerations of data center development, including reliable energy supply, natural resources (such as water availability to support cooling requirements), and relevant policy and regulatory considerations. Energy supply considerations are pivotal for data center operation. Between 2014 and 2018, data centers in the United States accounted for nearly 2 percent of the Nation’s total electricity consumption, and data center energy consumption is projected to increase from 2 to 6.7–12 percent of total U.S. electricity use by 2028. These energy requirements necessitate careful consideration of energy supply when considering potentially suitable locations for data center development. Experts anticipate that an increase in renewable energy generation will likely support most potential future power demand needs, including for data centers, followed by increases in natural gas, nuclear, and geothermal energy production. Additional capacity in the form of battery storage will likely not generate electricity, but may improve the reliability and flexibility of supply, helping to ensure that growing data center loads can be met. However, the U.S. Department of Energy estimates that the United States will need, on average, 57 percent more energy transmission infrastructure by 2035 to account for the growing power demand introduced by development such as data centers. Cooling server equipment in data centers requires large amounts of electricity and water, and this demand can be exacerbated by hot and humid conditions. Energy efficient water-based cooling technologies may reduce electricity consumption onsite but require more water consumption. This additional water demand has the potential to increase water stress and competition with other users. As such, developing data centers will likely need a thorough assessment of current and potential future water availability, as well as consideration of how water demand may change across other sectors. Data center development involves policy and regulatory considerations, as projects must undergo environmental review and authorization processes that can take 18–24 months. Coordinating these environmental reviews and authorizations with other energy development projects, such as building new transmission lines, may cause additional delays. Recent efforts by the U.S. Department of Energy and U.S. Department of the Interior aim to expedite environmental reviews and authorizations and improve coordination across agencies. The spatial analysis identified 771 existing AI data centers and more than 3,300 power plants. The spatial analysis found that 6 percent of AI data centers and 22 percent of power plants in the Western United States were on or within 1 mile of BLM lands, and California had the largest number of facilities. Most existing AI data centers were near high-voltage transmission lines and close to power plants, supporting efficient energy delivery. More than 90,000,000 acres of BLM lands were within 10 miles of existing high-voltage transmission lines, representing 38 percent of BLM lands in the study area. Available transmission infrastructure and the overlap with BLM lands varied by State, and Alaska had limited overlap compared to the rest of the Western United States. To operate most efficiently, data center temperatures must be at or below 80.6 degrees Fahrenheit. This analysis of future temperature and precipitation projections indicated increasing cooling demands for data centers, particularly in Arizona, California, and Nevada, where rising temperatures are expected to increase energy and operational costs while potentially stressing current regional electrical grid infrastructure. This report highlights relevant energy supply, natural resources, and regulatory considerations for data center development on BLM lands. This report does not provide a comprehensive ecological, regulatory, land suitability, or permitting analysis. The factors described here are contextual considerations only and are not intended to identify, rank, quantify, or recommend optimal areas for data center colocation. This spatial analysis focused solely on energy considerations relevant to data centers and did not consider water availability, critical habitats, BLM National Conservation Lands, areas of cultural or historical significance, and other sensitive resources. These topics are recognized as critical but were not within the scope of this science synthesis and spatial analysis.

Scientific Investigations Report↗

Alternative lifestyles: A plague persistence hypothesis

Several explanations have been posited for how the plague bacterium ( Yersinia pestis ) reemerges during sylvatic cycles within the same foci over many years, and often without direct evidence of host die-offs. One prevalent view is that transmission-optimized Y. pestis bacteria, exhibiting epizootic/enzootic behavior, almost continually replicate and survive through repeated, linked, host-centered propagation events. These bacteria, we will refer to as “ r-pestis ” type ecotype(s), represent a limited number of phenotypic lineages exhibiting optimal transmissibility and high rates of reproduction. These attributes, it is thought, assure their durability through time. For continuous r-pestis type expansions to be successful, adequate numbers of fleas and hosts must become infected to produce massive numbers of bacteria. In the process, host and flea numbers decline as they succumb to plague. Here we hypothesize that r-pestis population expansions seed the environment and confront a unique, highly competitive local milieu, where natural selection favors new ecotypes that incorporate a range of emergent adaptive survival strategies. These newly adapted survivors we recognize as a range of “ K - pestis ” ecotypes with greater durability and lower reproduction rates. These emergent K - pestis forms may arise in succession or coexist for varying periods of time with r-pestis ecotypes, and with other K - pestis ecotypes. Among K-pestis ecotypes, we hypothesize that through adaptive radiations, some persist within flea life stages, soil, organic waste, amoebae, plants, carcasses, hosts, or within niches yet to be characterized. In some settings, after a long quiet period, when favorable, K-pestis bacteria may trigger a singular event where an r-pestis transmission stream emerges precipitating another enzootic/epizootic progression. If this hypothesis withstands rigorous testing, then Y. pestis might represent an even more formidable, enduring, and adaptable foe, where unforeseen local events could trigger new epidemic and epizootic/enzootic events threatening humans and populations of other mammals, including those of conservation concern.

Ecosphere↗

Restoration handbook for sagebrush steppe ecosystems with emphasis on greater sage-grouse habitat—Part 1. Concepts for understanding and applying restoration

Sagebrush steppe ecosystems in the United States currently occur on only about one-half of their historical land area because of changes in land use, urban growth, and degradation of land, including invasions of non-native plants. The existence of many animal species depends on the existence of sagebrush steppe habitat. The greater sage-grouse ( Centrocercus urophasianus ) is a landscape-dependent bird that requires intact habitat and combinations of sagebrush and perennial grasses to exist. In addition, other sagebrush-obligate animals also have similar requirements and restoration of landscapes for greater sage-grouse also will benefit these animals. Once sagebrush lands are degraded, they may require restoration actions to make those lands viable habitat for supporting sagebrushobligate animals. This restoration handbook is the first in a three-part series on restoration of sagebrush ecosystems. In Part 1, we discuss concepts surrounding landscape and restoration ecology of sagebrush ecosystems and greater sage-grouse that habitat managers and restoration practitioners need to know to make informed decisions regarding where and how to restore specific areas. We will describe the plant dynamics of sagebrush steppe ecosystems and their responses to major disturbances, fire, and defoliation. We will introduce the concepts of ecosystem resilience to disturbances and resistance to invasions of annual grasses within sagebrush steppe. An introduction to soils and ecological site information will provide insights into the specific plants that can be restored in a location. Soil temperature and moisture regimes are described as a tool for determining resilience and resistance and the potential for various restoration actions. Greater sage-grouse are considered landscape birds that require large areas of intact sagebrush steppe; therefore, we describe concepts of landscape ecology that aid our decisions regarding habitat restoration. We provide a brief overview of restoration techniques for sage-grouse habitat restoration. We conclude with a description of the critical nature of monitoring for adaptive management of sagebrush steppe restoration at landscape- and project-specific levels.

Arizona, California, Colorado, Idaho, Montana, Neb↗

Probabilistic estimation of dune retreat on the Gold Coast, Australia

Sand dunes are an important natural buffer between storm impacts and development backing the beach on the Gold Coast of Queensland, Australia. The ability to forecast dune erosion at a prediction horizon of days to a week would allow efficient and timely response to dune erosion in this highly populated area. Towards this goal, we modified an existing probabilistic dune erosion model for use on the Gold Coast. The original model was trained using observations of dune response from Hurricane Ivan on Santa Rosa Island, Florida, USA (Plant and Stockdon 2012. Probabilistic prediction of barrier-island response to hurricanes, Journal of Geophysical Research, 117(F3), F03015). The model relates dune position change to pre-storm dune elevations, dune widths, and beach widths, along with storm surge and run-up using a Bayesian network. The Bayesian approach captures the uncertainty of inputs and predictions through the conditional probabilities between variables. Three versions of the barrier island response Bayesian network were tested for use on the Gold Coast. One network has the same structure as the original and was trained with the Santa Rosa Island data. The second network has a modified design and was trained using only pre- and post-storm data from 1988-2009 for the Gold Coast. The third version of the network has the same design as the second version of the network and was trained with the combined data from the Gold Coast and Santa Rosa Island. The two networks modified for use on the Gold Coast hindcast dune retreat with equal accuracy. Both networks explained 60% of the observed dune retreat variance, which is comparable to the skill observed by Plant and Stockdon (2012) in the initial Bayesian network application at Santa Rosa Island. The new networks improved predictions relative to application of the original network on the Gold Coast. Dune width was the most important morphologic variable in hindcasting dune retreat, while hydrodynamic variables, surge and run-up elevation, were also important

Queensland↗

Predicting large hydrothermal systems

We train five models using two machine learning (ML) regression algorithms (i.e., linear regression and XGBoost) to predict hydrothermal upflow in the Great Basin. Feature data are extracted from datasets supporting the INnovative Geothermal Exploration through Novel Investigations Of Undiscovered Systems project (INGENIOUS). The label data (the reported convective signals) are extracted from measured thermal gradients in wells by comparing the total estimated heat flow at the wells to the modeled background conductive heat flow. That is, the reported convective signal is the difference between the background conductive heat flow and the well heat flow. The reported convective signals contain outliers that may affect upflow prediction, so the influence of outliers is tested by constructing models for two cases: 1) using all the data (i.e., -91 to 11,105 mW/m2), and 2) truncating the range of labels to include only reported convective signals between -25 and 200 mW/m2. Because hydrothermal systems are sparse, models that predict high convective signal in smaller areas better match the natural frequency of hydrothermal systems. Early results demonstrate that XGBoost outperforms linear regression. For XGBoost using the truncated range of labels, half of the high reported signals are within < 3 % of the highest predictions. For XGBoost using the entire range of labels, half of the high reported signals are in < 13 % of the highest predictions. While this implies that the truncated regression is superior, the all-data model better predicts the locations of power-producing systems (i.e., the operating power plants are in a smaller fraction of the study area given by the highest predictions). Even though the models generally predict greater hydrothermal upflow for higher reported convective signals than for lower reported convective signals, both XGBoost models consistently underpredict the magnitude of higher signals. This behavior is attributed to low resolution/granularity of input features compared with the scale of a hydrothermal upflow zone (a few km or less across). Trouble estimating exact values while still reliably predicting high versus low convective signals suggests that a future strategy such as ranked ordinal regression (e.g., classifying into ordered bins for low, medium, high, and very high convective signal) might fit better models, since doing so reduces problems introduced by outliers while preserving the property of larger versus smaller signals.

Geothermal Resources Council Transactions↗

Occurrence, temporal variation, and estrogenic burden of five parabens in sewage sludge collected across the United States

Five parabens used as preservatives in pharmaceuticals and personal care products (PPCPs) were measured in sewage sludges collected at 14 U.S. wastewater treatment plants (WWTPs) located in nine states. Detected concentration ranges (ng/g, dry weight) and frequencies were as follows: methyl paraben (15.9 to 203.0; 100%), propyl paraben (0.5 to 7.7; 100%), ethyl paraben (< 0.6 to 2.6; 63%), butyl paraben (< 0.4 to 4.3; 42%) and benzyl paraben (< 0.4 to 3.3; 26%). The estrogenicity inherent to the sum of parabens detected in sewage sludge (ranging from 10.1 to 500.1 pg/kg 17 β- estradiol equivalents) was insignificant when compared to the 10 6 -times higher value calculated for natural estrogens reported in the literature to occur in sewage sludge. Temporal monitoring at one WWTP provided insights into temporal and seasonal variations in paraben concentrations. This is the first report on the occurrence of five parabens in sewage sludges from across the U.S., and internationally, the first on temporal variations of paraben levels in sewage sludge. Study results will help to inform the risk assessment of sewage sludge destined for land application (biosolids).

Arizona, Indiana, Florida, Maryland, Montana, New ↗

Cloud cover and delayed herbivory relative to timing of spring onset interact to dampen climate change impacts on net ecosystem exchange in a coastal Alaskan wetland

Rapid warming in northern ecosystems over the past four decades has resulted in earlier spring, increased precipitation, and altered timing of plant–animal interactions, such as herbivory. Advanced spring phenology can lead to longer growing seasons and increased carbon (C) uptake. Greater precipitation coincides with greater cloud cover possibly suppressing photosynthesis. Timing of herbivory relative to spring phenology influences plant biomass. None of these changes are mutually exclusive and their interactions could lead to unexpected consequences for Arctic ecosystem function. We examined the influence of advanced spring phenology, cloud cover, and timing of grazing on C exchange in the Yukon–Kuskokwim Delta of western Alaska for three years. We combined advancement of the growing season using passive-warming open-top chambers (OTC) with controlled timing of goose grazing (early, typical, and late season) and removal of grazing. We also monitored natural variation in incident sunlight to examine the C exchange consequences of these interacting forcings. We monitored net ecosystem exchange of C (NEE) hourly using an autochamber system. Data were used to construct daily light curves for each experimental plot and sunlight data coupled with a clear-sky model was used to quantify daily and seasonal NEE over a range of incident sunlight conditions. Cloudy days resulted in the largest suppression of NEE, reducing C uptake by approximately 2 g C m −2 d −1 regardless of the timing of the season or timing of grazing. Delaying grazing enhanced C uptake by approximately 3 g C m −2 d −1 . Advancing spring phenology reduced C uptake by approximately 1.5 g C m −2 d −1 , but only when plots were directly warmed by the OTCs; spring advancement did not have a long-term influence on NEE. Consequently, the two strongest drivers of NEE, cloud cover and grazing, can have opposing effects and thus future growing season NEE will depend on the magnitude of change in timing of grazing and incident sunlight.

Alaska↗

Plague bacterium as a transformer species in prairie dogs and the grasslands of western North America

Invasive transformer species change the character, condition, form, or nature of ecosystems and deserve considerable attention from conservation scientists. We applied the transformer species concept to the plague bacterium Yersinia pestis in western North America, where the pathogen was introduced around 1900. Y. pestis transforms grassland ecosystems by severely depleting the abundance of prairie dogs ( Cynomys spp. ) and thereby causing declines in native species abundance and diversity, including threatened and endangered species; altering food web connections; altering the import and export of nutrients; causing a loss of ecosystem resilience to encroaching invasive plants; and modifying prairie dog burrows. Y. pestis poses an important challenge to conservation biologists because it causes trophic-level perturbations that affect the stability of ecosystems. Unfortunately, understanding of the effects of Y. pestis on ecosystems is rudimentary, highlighting an acute need for continued research.

Conservation Biology↗

Using noninvasive genetics for estimating density and assessing diet of urban and rural coyotes in Florida, USA

Coyotes ( Canis latrans ) are expanding their range and due to conflicts with the public and concerns of Coyotes affecting natural resources such as game or sensitive species, there is interest and often a demand to monitor Coyote populations. A challenge to monitoring is that traditional invasive methods involving live-capture of individual animals are costly and can be controversial. Natural resource management agencies can benefit from contemporary noninvasive genetic sampling approaches aimed at determining key aspects of Coyote ecology (e.g., population density and food habits). However, the efficacy of such approaches under different environmental conditions is poorly understood. Our objectives were to 1) examine accumulation and nuclear DNA degradation rates of Coyote scats in metropolitan and rural sites in Florida to help optimize methods to estimate population density; and 2) explore new genetic methods for determining diet of Coyotes based on vertebrate, plant, and invertebrate species DNA identified in scat. Recently developed DNA metabarcoding approaches make it possible to simultaneously identify DNA from multiple prey species in predator scat samples, but an exploration of this tool for assessing Coyote diet has not been pursued. We observed that scat accumulation rates (0.02 scats/km/day) did not vary between sites and fecal DNA amplification success decreased and genotyping errors increased over time with exposure to sun and precipitation. DNA sampling allowed us to generate a Coyote density estimate for the urban environment of eight Coyotes per 100 km2, but lack of recaptures in the rural area precluded density estimation. DNA metabarcoding showed promise for assessing diet contributions of vertebrate species to Coyote diet. Feral Swine (Sus scrofa) were detected as prey at higher frequencies than previously reported. We identify several considerations that can be used to optimize future noninvasive sampling efforts for Coyotes in the southeastern United States. We also discuss strengths and drawbacks of utilizing DNA metabarcoding for assessing diet of generalist carnivores such as Coyotes.

Florida↗

Long-term changes in ground water chemistry at a phytoremediation demonstration site

A field-scale demonstration project was conducted to evaluate the capability of eastern cottonwood trees ( Populus deltoides ) to attenuate trichloroethene (TCE) contamination of ground water. By the middle of the sixth growing season, trees planted where depth to water was <3 m delivered enough dissolved organic carbon to the underlying aquifer to lower dissolved oxygen concentrations, to create iron-reducing conditions along the plume centerline and sulfate-reducing or methanogenic conditions in localized areas, and to initiate in situ reductive dechlorination of TCE. Apparent biodegradation rate constants for TCE along the centerline of the plume beneath the phytoremediation system increased from 0.0002/d to 0.02/d during the first six growing seasons. The corresponding increase in natural attenuation capacity of the aquifer along the plume centerline, from 0.0004/m to 0.024/m, is associated with a potential decrease in plume-stabilization distance from 9680 to 160 m. Demonstration results provide insight into the amount of vegetation and time that may be needed to achieve cleanup objectives at the field scale.

Ground Water↗

Effects of flow modification on a cattail wetland at the mouth of Irondequoit Creek near Rochester, New York: Water levels, wetland biota, sediment, and water quality

An 11-year (1990-2001) study of the Ellison Park wetland, a 423-acre, predominantly cattail (Typha glauca) wetland at the mouth of Irondequoit Creek, was conducted to document the effects that flow modifications, including installation of a flow-control structure (FCS) in 1997 and increased diversion of stormflows to the backwater areas of the wetland, would have on the wetland's ability to decrease chemical loads transported by Irondequoit Creek into Irondequoit Bay on Lake Ontario. The FCS was designed to raise the water-surface elevation and thereby increase the dispersal and detention of stormflows in the upstream half of the wetland; this was expected to promote sedimentation and microbial utilization of nutrients, and thereby decrease the loads of certain constituents, primarily phosphorus, that would otherwise be carried into Irondequoit Bay. An ecological monitoring program was established to document changes in the wetland's water levels, biota, sedimentation rates, and chemical quality of water and sediment that might be attributable to the flow modifications. Water-level increases during storms were mostly confined to the wetland area, within about 5,000 ft upstream from the FCS. Backwater at a point of local concern, about 13,000 ft upstream, was due to local debris jams or constriction of flow by bridges and was not attributable to the FCS. Plant surveys documented species richness, concentrations of nutrients and metals in cattail tissues, and cattail productivity. Results indicated that observed differences among survey periods and between the areas upstream and downstream from the FCS were due to seasonal changes in water levels—either during the current year or at the end of the previous year's growing season—that reflected the water-surface elevation of Lake Ontario, rather than water-level control by the FCS. Results showed no adverse effects from the naturally high water levels that prevail annually during the spring and summer in the wetland, nor from the short-duration increases in water levels that result from FCS operation. Fish surveys documented the use of the wetland by 44 species, of which 25 to 29 species were found in any given year. Community composition was relatively consistent during the study, but seasonal and year-to-year variations in dominant resident and nonresident species were noted, and probably reflected natural or regional population patterns in Lake Ontario and Irondequoit Bay. The FCS allowed fish passage at all water levels and had no discernible adverse effect on the fish community. Bird surveys documented the use of the wetland by more than 90 species for breeding, feeding, and migration. Ground-nesting birds were unaffected by the FCS. Seasonally high water levels, rather than short-duration increases caused by the FCS, might have caused the scarcity or absence of certain wetland species by limiting the extent of breeding habitat for some species and the exposure of mud flats that attracted other species. Some noticeably scarce or absent species also were rare or absent elsewhere along the south-central shore of Lake Ontario. Benthic-macroinvertebrate studies were of minimal use for evaluating the effect of the FCS because no surveys were conducted after FCS installation. The precontrol results allowed assessment of the ecological quality of the wetland on the basis of biotic indices, and generally indicated moderately to severely impaired conditions. Differences between the macroinvertebrate communities in the southern part of the wetland and those in the northern part were attributed to habitat differences, such as substrate composition, water depth, and density of submerged aquatic vegetation. Sedimentation rates in the areas upstream and downstream from the FCS increased after the flow modifications, more in the area upstream from the FCS than in the downstream area. The concurrent downstream increase and the dynamic patterns of deposition and scour indicated that although the FCS and the other flow modifications undoubtedly were major factors in the postcontrol upstream increase in sedimentation rates, other factors, such as the magnitude, frequency, and the timing (season) of peak flows, might also have contributed. Periodic analyses of sediment samples from three longterm depositional sites in the wetland documented the concentrations of major and trace elements, polycyclic aromatic hydrocarbons, and organochlorine and organophosphate compounds. The concentrations of most constituents showed no substantial fluctuation or consistent upward or downward trend during the years sampled, nor did they identify any change after FCS installation. Comparison of the measured concentrations with sediment-quality guidelines that are used to assess the ecological quality of substrate environments indicated that the wetland was moderately to severely impaired—an assessment consistent with the benthic-macroinvertebrate biotic indices. During the precontrol period (1990–96), the wetland was a sink for particulate constituents (removal efficiencies for total phosphorus and total suspended solids were 28 and 47 percent, respectively), but had little effect on conservative constituents (chloride and sulfate). The wetland was a source of orthophosphate and ammonia (removal efficiencies were -38 and -84 percent, respectively). During the postcontrol period (1997–2001), the wetland continued to be a sink for particulate constituents (removal efficiencies for total phosphorus and total suspended solids were 45 and 52 percent, respectively); the exportation of orthophosphate by the wetland decreased (by 7 percent), whereas that of ammonia increased (by about 70 percent). The outflow loads of orthophosphate and ammonia represented about 15 and 2.3 percent of total phosphorus and total nitrogen loads, respectively. Changes in the loads of conservative constituents were negligible, and the overall removal efficiencies for other constituents during the precontrol period differed from those of the postcontrol period by no more than 5.4 percent. Statistical analyses of monthly inflow and outflow loads indicated significant differences between inflow and outflow loads of most constituents during the pre- and postcontrol periods. Load data were adjusted to remove the effects of dissimilar hydrologic conditions that prevailed during the pre- and postcontrol periods, and to isolate the water-quality-improvement effect that could be attributed solely to the FCS. Results indicated that the FCS contributed significantly to the decrease in total phosphorus loads, and slightly to a decrease in ammonia-plus-organic nitrogen loads, but had little or no significant effect on loads of other constituents.

New York↗

Natural and anthropogenic geochemical tracers to investigate residence times and groundwater–surface-water interactions in an urban alluvial aquifer

A multi-component geochemical dataset was collected from groundwater and surface-water bodies associated with the urban Fountain Creek alluvial aquifer, Colorado, USA, to facilitate analysis of recharge sources, geochemical interactions, and groundwater-residence times. Results indicate that groundwater can be separated into three distinct geochemical zones based on location within the flow system and proximity to surface water, and these zones can be used to infer sources of recharge and groundwater movement through the aquifer. Rare-earth-element concentrations and detections of wastewater-indicator compounds indicate the presence of effluent from wastewater-treatment plants in both groundwater and surface water. Effluent presence in groundwater indicates that streams in the area lose to groundwater in some seasons and are a source of focused groundwater recharge. Distributions of pharmaceuticals and wastewater-indicator compounds also inform an understanding of groundwater–surface-water interactions. Noble-gas isotopes corroborate rare-earth-element data in indicating geochemical evolution within the aquifer from recharge area to discharge area and qualitatively indicate variable groundwater-residence times and mixing with pre-modern groundwater. Quantitative groundwater-residence times calculated from 3 H/ 3 He, SF 6 , and lumped-parameter modeling generally are less than 20 years, but the presence of mixing with older groundwater of an unknown age is also indicated at selected locations. Future investigations would benefit by including groundwater-age tracers suited to quantification of mixing for both young (years to decades) and old (centuries and millennia) groundwater. This multi-faceted analysis facilitated development of a conceptual model for the investigated groundwater-flow system and illustrates the application of an encompassing suite of analytes in exploring hydrologic and geochemical interactions in complex systems.

Colorado↗

Buzzards Bay salt marshes: Vulnerability and adaptation potential

Salt marshes with lush grass meadows teeming with shorebirds are iconic features of the Buzzards Bay coast and provide opportunities for recreation, aesthetic enjoyment, as well as important environmental benefits. These productive coastal wetlands are important because they protect properties from storm surges, remove nutrients from the water and carbon from the atmosphere, and provide critical habitats for fish, shellfish, and birds. Found where the land meets the sea, salt marshes are naturally dynamic features that change with rising seas, waves, ice, and storms. In the past, humans purposely altered salt marshes by filling them to create buildable land or digging drainage ditches. These major alterations harmed marsh structure and health. In recent decades, however, marshes are degrading because of more diffuse and complex pressures such as nutrient pollution, sea level rise, major storms, and crab overgrazing. As a result, at many places along the East Coast, marshes have crumbling banks and large areas where the plants have died, leaving behind mudflats. The Buzzards Bay Coalition and the Buzzards Bay National Estuary Program began field monitoring of salt marshes around Buzzards Bay in 2019 to document changes (map below shows sites). We partnered with the U.S. Geological Survey and the Woodwell Climate Research Center to use aerial tools to investigate how different characteristics of the long-term marsh sites and their watersheds affect the marsh’s current health and likely future. This report brings together the results of on the ground monitoring with data from aerial imagery to look at marsh status at 12 long-term monitoring sites based on existing stressors, current marsh conditions, and potential for adaptation.

Massachusetts↗

Investigation of submarine groundwater discharge along the tidal reach of the Caloosahatchee River, southwest Florida

The tidal reach of the Caloosahatchee River is an estuarine habitat that supports a diverse assemblage of biota including aquatic vegetation, shellfish, and finfish. The system has been highly modified by anthropogenic activity over the last 150 years (South Florida Water Management District (SFWMD), 2009). For example, the river was channelized and connected to Lake Okeechobee in 1881 (via canal C-43). Subsequently, three control structures (spillway and locks) were installed for flood protection (S-77 and S-78 in the 1930s) and for saltwater-intrusion prevention (S-79, W.P. Franklin Lock and Dam in 1966). The emplacement of these structures and their impact to natural water flow have been blamed for water-quality problems downstream within the estuary (Flaig and Capece, 1998; SFWMD, 2009). Doering and Chamberlain (1999) found that the operation of these control structures caused large and often rapid variations in salinity during various times of the year. Variable salinities could have deleterious impacts on the health of organisms in the Caloosahatchee River estuary. Flow restriction along the Caloosahatchee has also been linked to surface-water eutrophication problems (Doering and Chamberlain, 1999; SFWMD, 2009) and bottom-sediment contamination (Fernandez and others, 1999). Sources of nutrients (nitrogen and phosphorous) that cause eutrophication are primarily from residential sources and agriculture, though wastewater-treatment-plant discharges can also play a major role (SFWMD, 2009). The pathway for many of these nutrients is by land runoff and direct discharge from stormwater drains. An often overlooked source of nutrients and other chemical constituents is from submarine groundwater discharge (SGD). SGD can be either a diffuse or point source (for example, submarine springs) of nutrients and other chemical constituents to coastal waters (Valiela and others, 1990; Swarzenski and others, 2001; 2006; 2007; 2008). SGD can be composed of either fresh or marine water or various mixed ratios of fresh and marine water (Martin and others, 2007). In coastal areas where water-table elevations (hydraulic gradients) are steep, such as in Hood Canal, Washington (Swarzenski and others, 2007; Simonds and others, 2008), groundwater entering the coastal marine waters can be fresh (~1-4 parts per thousand, ppt). SGD in coastal locations that have low relief (low hydraulic gradients) such as the study area or other locations in Florida are typically driven by tidal pumping (Reich and others, 2002; 2008; Swarzenski and others, 2008), and water advecting into surface water is composed of recirculated marine water mixed with either fresh or brackish groundwaters. The importance of SGD in the delivery of nutrients and trace elements to coastal environments has been shown to be both beneficial and deleterious to ecosystem health (Valiela and others, 1990). The logical step in studying SGD is to map areas where SGD occurs. Methods such as continuous surface-water radon-222 ( 222 Rn) mapping and electrical resistivity (continuous resistivity profiles, CRP) have been developed and used to identify potential SGD sites (Dulaiova and others, 2005; Swarzenski and others 2004; 2006; 2007; 2008; Reich and others, 2008). CRP data record subsurface, bulk-resistivity measurements to depths up to 25 meters (m). The bulk resistivity can be representative of changes in porewater salinity or in lithology (Reich and others, 2008; Swarzenski and others, 2008). Radon-222 (half-life = 3.28 days) is a natural tracer of groundwater, because sediments and rocks, containing uranium-bearing materials such as limestone and phosphatic material, continually produce 222 Rn. Rn-222 (also referred to simply as radon) is an ideal tracer, because there is a constant source. Since radon is a gas, 222 Rn does not build up in the surface water but rather evades directly to the atmosphere (Burnett and Dulaiova, 2003; Burnett and others, 2003; Dulaiova and Burnett, 2006).

Florida↗

A meta-analysis highlights the idiosyncratic nature of tradeoffs in laboratory models of virus evolution

Different theoretical frameworks have been invoked to guide the study of virus evolution. Three of the more prominent ones are (i) the evolution of virulence, (ii) life history theory, and (iii) the generalism–specialism dichotomy. All involve purported tradeoffs between traits that define the evolvability and constraint of virus-associated phenotypes. However, as popular as these frameworks are, there is a surprising paucity of direct laboratory tests of the frameworks that support their utility as broadly applicable theoretical pillars that can guide our understanding of disease evolution. In this study, we conduct a meta-analysis of direct experimental evidence for these three frameworks across several widely studied virus–host systems: plant viruses, fungal viruses, animal viruses, and bacteriophages. We extracted 60 datasets from 28 studies and found a range of relationships between traits in different analysis categories (e.g., frameworks, virus–host systems). Our work demonstrates that direct evidence for relationships between traits is highly idiosyncratic and specific to the host–virus system and theoretical framework. Consequently, scientists researching viral pathogens from different taxonomic groups might reconsider their allegiance to these canons as the basis for expectation, explanation, or prediction. Future efforts could benefit from consistent definitions, and from developing frameworks that are compatible with the evidence and apply to particular biological and ecological contexts.

Virus Evolution↗

Ecosystem history: Terrestrial and fresh-water ecosystems of southern Florida

Introduction Plant and animal communities of the historic Everglades have undergone striking changes over the last few decades, including declines in wading bird populations, invasion by exotic (non-native) plant and animal species, and areal expansion of cattails into sawgrass marshes. Many of these changes have been attributed to human activities in the region, and efforts are underway to restore the Everglades to a more pristine state. Restoration plans include alteration of the present levee and canal system that controls water flow throughout southern Florida and changes in agricultural and land development activities. However, can we be sure that the observed changes are related to human practices rather than representing an extreme in the natural variability of the system? This question should be answered before changing current land-use practices to alleviate environmental concerns. To address this question, it is necessary to look at patterns of floral and faunal change over the last century as well as over the last few millennia. This project aims to reconstruct floral and faunal composition at selected time intervals throughout southern Florida to determine: 1) detailed biotic changes over the last 150 years; 2) the natural range of biotic variability over the last few thousand years; and 3) determine whether any cause-and-effect relationships exist between biotic and environmental changes. Such questions are being addressed by scientists in the South Florida Initiative of the Ecosystem Program at the U.S. Geological Survey.

Florida↗