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Effects of plant phenology and vertical height on accuracy of radio-telemetry locations

The use of very high frequency (VHF) radio-telemetry remains wide-spread in studies of wildlife ecology and management. However, few studies have evaluated the influence of vegetative obstruction on accuracy in differing habitats with varying transmitter types and heights. Using adult and fawn collars at varying heights above the ground (0, 33, 66 and 100 cm) to simulate activities (bedded, feeding and standing) and ages (neonate, juvenile and adult) of deer Odocoileus spp., we collected 5,767 bearings and estimated 1,424 locations (28-30 for each of 48 subsamples) in three habitat types (pasture, grassland and forest), during two stages of vegetative growth (spring and late summer). Bearing error was approximately twice as large at a distance of 900 m for fawn (9.9°) than for adult deer collars (4.9°). Of 12 models developed to explain the variation in location error, the analysis of covariance model (HT*D + C*D + HT*TBA + C*TBA) containing interactions of height of collar above ground (HT), collar type (C), vertical height of understory vegetation (D) and tree basal area (TBA) was the best model (w i = 0.92) and explained ∼ 71% of the variation in location error. Location error was greater for both collar types at 0 and 33 cm above the ground compared to 66 and 100 cm above the ground; however, location error was less for adult than fawn collars. Vegetation metrics influenced location error, which increased with greater vertical height of understory vegetation and tree basal area. Further, interaction of vegetation metrics and categorical variables indicated significant effects on location error. Our results indicate that researchers need to consider study objectives, life history of the study animal, signal strength of collar (collar type), distance from transmitter to receiver, topographical changes in elevation, habitat composition and season when designing telemetry protocols. Bearing distances in forested habitat should be decreased (approximately 23% in our study) compared to bearing distances in open habitat to maintain a consistent bearing error across habitats. Additionally, we believe that field biologists monitoring neonate ungulates for habitat selection should rely on visual locations rather than using VHF-collars and triangulation.

South Dakota↗

Predicting invasion in grassland ecosystems: Is exotic dominance the real embarrassment of richness?

Invasions have increased the size of regional species pools, but are typically assumed to reduce native diversity. However, global-scale tests of this assumption have been elusive because of the focus on exotic species richness, rather than relative abundance. This is problematic because low invader richness can indicate invasion resistance by the native community or, alternatively, dominance by a single exotic species. Here, we used a globally replicated study to quantify relationships between exotic richness and abundance in grass-dominated ecosystems in 13 countries on six continents, ranging from salt marshes to alpine tundra. We tested effects of human land use, native community diversity, herbivore pressure, and nutrient limitation on exotic plant dominance. Despite its widespread use, exotic richness was a poor proxy for exotic dominance at low exotic richness, because sites that contained few exotic species ranged from relatively pristine (low exotic richness and cover) to almost completely exotic-dominated ones (low exotic richness but high exotic cover). Both exotic cover and richness were predicted by native plant diversity (native grass richness) and land use (distance to cultivation). Although climate was important for predicting both exotic cover and richness, climatic factors predicting cover (precipitation variability) differed from those predicting richness (maximum temperature and mean temperature in the wettest quarter). Herbivory and nutrient limitation did not predict exotic richness or cover. Exotic dominance was greatest in areas with low native grass richness at the site- or regional-scale. Although this could reflect native grass displacement, a lack of biotic resistance is a more likely explanation, given that grasses comprise the most aggressive invaders. These findings underscore the need to move beyond richness as a surrogate for the extent of invasion, because this metric confounds monodominance with invasion resistance. Monitoring species' relative abundance will more rapidly advance our understanding of invasions

Global Change Biology↗

Macroecological patterns of sexual size dimorphism in turtles of the world

Sexual size dimorphism (SSD) is a well-documented phenomenon in both plants and animals; however, the ecological and evolutionary mechanisms that drive and maintain SSD patterns across geographic space at regional and global scales are understudied, especially for reptiles. Our goal was to examine geographic variation of turtle SSD and to explore ecological and environmental correlates using phylogenetic comparative methods. We use published body size data on 135 species from nine turtle families to examine how geographic patterns and the evolution of SSD are influenced by habitat specialization, climate (annual mean temperature and annual precipitation) and climate variability, latitude, or a combination of these predictor variables. We found that geographic variation, magnitude and direction of turtle SSD are best explained by habitat association, annual temperature variance and annual precipitation. Use of semi-aquatic and terrestrial habitats was associated with male-biased SSD, whereas use of aquatic habitat was associated with female-biased SSD. Our results also suggest that greater temperature variability is associated with female-biased SSD. In contrast, wetter climates are associated with male-biased SSD compared with arid climates that are associated with female-biased SSD. We also show support for a global latitudinal trend in SSD, with females being larger than males towards the poles, especially in the families Emydidae and Geoemydidae. Estimates of phylogenetic signal for both SSD and habitat type indicate that closely related species occupy similar habitats and exhibit similar direction and magnitude of SSD. These global patterns of SSD may arise from sex-specific reproductive behaviour, fecundity and sex-specific responses to environmental factors that differ among habitats and vary systematically across latitude. Thus, this study adds to our current understanding that while SSD can vary dramatically across and within turtle species under phylogenetic constraints, it may be driven, maintained and exaggerated by habitat type, climate and geographic location.

Journal of Evolutionary Biology↗

An ecological response model for the Cache la Poudre River through Fort Collins

The Poudre River Ecological Response Model (ERM) is a collaborative effort initiated by the City of Fort Collins and a team of nine river scientists to provide the City with a tool to improve its understanding of the past, present, and likely future conditions of the Cache la Poudre River ecosystem. The overall ecosystem condition is described through the measurement of key ecological indicators such as shape and character of the stream channel and banks, streamside plant communities and floodplain wetlands, aquatic vegetation and insects, and fishes, both coolwater trout and warmwater native species. The 13- mile-long study area of the Poudre River flows through Fort Collins, Colorado, and is located in an ecological transition zone between the upstream, cold-water, steep-gradient system in the Front Range of the Southern Rocky Mountains and the downstream, warm-water, low-gradient reach in the Colorado high plains. The City wanted to better understand the ecological response of the Poudre River ecosystem to potential changes in stream flow and other physical parameters through the conceptual framework of a multivariable integrated model. This goal was met through the use of a probabilistic model based on Bayesian concepts. This construct allowed the integration of a wide range of data and expert opinion (as informed by local data) to predict potential changes to ecosystem conditions under various flow scenarios. Nine flow scenarios representing past, present, and possible future hydrology were developed as the primary model input. Both reach-scale drivers such as stream channel conditions and pollutant loads, as well as ecological conditions, including species composition, interactions, and habitat requirements influenced model-predicted ecosystem outcomes. Model output consisted of probability distributions for eight ecological indicators collectively representing the physical setting, aquatic life, and riparian habitats of the river ecosystem. We are confident in model predictions related to probable trends, relative magnitude of changes and potential ecosystem responses to changing flow conditions, though data availability and the process of converting diverse data types into a common unit (probabilities) limit precision of individual results. Key findings suggest that: The present ecological function of the Poudre River is altered as a result of more than 150 years of human influences that include highly managed flows, urbanization, gravel mining, channelization and urban and industrial encroachment in the floodplain, underscoring the vulnerable and complex character of the Poudre River; A continuation of today’s flow management will lead to ongoing changes in ecosystem condition, and additional water depletions will compromise ecological conditions; High flows play an essential role in maintaining and improving the aquatic and riparian condition of the river; Adequate flows in base-flow periods are critical to desirable water quality, and thriving fish and insect populations; Improvement of native aquatic life is possible if issues related to channel modifications, siltation, invasive species, and base and high flow conditions are managed properly; The present confined river channel and modified flows has reduced the potential for a keystone and iconic species, plains cottonwood, to be self-sustaining in the study area; The streamside corridor retains the potential to support a functioning riparian forest that provides important ecological services if periodic floodplain inundation occurs. Environmental flows that combine stable and adequate flows in base-flow periods with occasional rejuvenating high flows that meet target levels defined in this study are likely improve all biological indicators across the system. ERM test scenarios that include both stable base flows and rejuvenating high flows indicate that substantial improvements in the river ecosystem can be achieved with improved management of flow volumes similar to those observed in the river during the last half century of intensive water development. These results underscore the possibility of improving the river ecosystem through active management while still maintaining the Poudre’s diverse economic benefits and role as a working river. The ERM was designed to represent the multi-dimensional ecological character of the contemporary urban Poudre River. It provides a scientific foundation that can serve as a decision support tool and foster a more informed community discussion about the future of the river as it provides a better understanding of the likely response of the Poudre River ecosystem to environmental flow management and other stewardship activities. In particular, model results can assist managers in developing specific management actions to achieve desirable goals for key indicators of river health.

Colorado↗

Nitrogen deposition weakens soil carbon control of nitrogen dynamics across the contiguous United States

Anthropogenic nitrogen (N) deposition is unequally distributed across space and time, with inputs to terrestrial ecosystems impacted by industry regulations and variations in human activity. Soil carbon (C) content normally controls the fraction of mineralized N that is nitrified ( ƒ nitrified ), affecting N bioavailability for plants and microbes. However, it is unknown whether N deposition has modified the relationships among soil C, net N mineralization, and net nitrification. To test whether N deposition alters the relationship between soil C and net N transformations, we collected soils from coniferous and deciduous forests, grasslands, and residential yards in 14 regions across the contiguous United States that vary in N deposition rates. We quantified rates of net nitrification and N mineralization, soil chemistry (soil C, N, and pH), and microbial biomass and function (as beta-glucosidase (BG) and N -acetylglucosaminidase (NAG) activity) across these regions. Following expectations, soil C was a driver of ƒ nitrified across regions, whereby increasing soil C resulted in a decline in net nitrification and ƒ nitrified . The ƒ nitrified value increased with lower microbial enzymatic investment in N acquisition (increasing BG:NAG ratio) and lower active microbial biomass, providing some evidence that heterotrophic microbial N demand controls the ammonium pool for nitrifiers. However, higher total N deposition increased ƒ nitrified , including for high soil C sites predicted to have low ƒ nitrified , which decreased the role of soil C as a predictor of ƒ nitrified . Notably, the drop in contemporary atmospheric N deposition rates during the 2020 COVID-19 pandemic did not weaken the effect of N deposition on relationships between soil C and ƒ nitrified . Our results suggest that N deposition can disrupt the relationship between soil C and net N transformations, with this change potentially explained by weaker microbial competition for N. Therefore, past N inputs and soil C should be used together to predict N dynamics across terrestrial ecosystems.

contiguous United States↗

A pan-Arctic synthesis of CH 4 and CO 2 production from anoxic soil incubations

Permafrost thaw can alter the soil environment through changes in soil moisture, frequently resulting in soil saturation, a shift to anaerobic decomposition, and changes in the plant community. These changes, along with thawing of previously frozen organic material, can alter the form and magnitude of greenhouse gas production from permafrost ecosystems. We synthesized existing methane (CH 4 ) and carbon dioxide (CO 2 ) production measurements from anaerobic incubations of boreal and tundra soils from the geographic permafrost region to evaluate large-scale controls of anaerobic CO 2 and CH 4 production and compare the relative importance of landscape-level factors (e.g., vegetation type and landscape position), soil properties (e.g., pH, depth, and soil type), and soil environmental conditions (e.g., temperature and relative water table position). We found fivefold higher maximum CH 4 production per gram soil carbon from organic soils than mineral soils. Maximum CH 4 production from soils in the active layer (ground that thaws and refreezes annually) was nearly four times that of permafrost per gram soil carbon, and CH 4 production per gram soil carbon was two times greater from sites without permafrost than sites with permafrost. Maximum CH 4 and median anaerobic CO 2 production decreased with depth, while CO 2 :CH 4 production increased with depth. Maximum CH 4 production was highest in soils with herbaceous vegetation and soils that were either consistently or periodically inundated. This synthesis identifies the need to consider biome, landscape position, and vascular/moss vegetation types when modeling CH 4 production in permafrost ecosystems and suggests the need for longer-term anaerobic incubations to fully capture CH 4 dynamics. Our results demonstrate that as climate warms in arctic and boreal regions, rates of anaerobic CO 2 and CH 4 production will increase, not only as a result of increased temperature, but also from shifts in vegetation and increased ground saturation that will accompany permafrost thaw.

Global Change Biology↗

Intercomparison, interpretation, and assessment of spring phenology in North America estimated from remote sensing for 1982-2006

Shifts in the timing of spring phenology are a central feature of global change research. Long-term observations of plant phenology have been used to track vegetation responses to climate variability but are often limited to particular species and locations and may not represent synoptic patterns. Satellite remote sensing is instead used for continental to global monitoring. Although numerous methods exist to extract phenological timing, in particular start-of-spring (SOS), from time series of reflectance data, a comprehensive intercomparison and interpretation of SOS methods has not been conducted. Here, we assess 10 SOS methods for North America between 1982 and 2006. The techniques include consistent inputs from the 8 km Global Inventory Modeling and Mapping Studies Advanced Very High Resolution Radiometer NDVIg dataset, independent data for snow cover, soil thaw, lake ice dynamics, spring streamflow timing, over 16 000 individual measurements of ground-based phenology, and two temperature-driven models of spring phenology. Compared with an ensemble of the 10 SOS methods, we found that individual methods differed in average day-of-year estimates by ±60 days and in standard deviation by ±20 days. The ability of the satellite methods to retrieve SOS estimates was highest in northern latitudes and lowest in arid, tropical, and Mediterranean ecoregions. The ordinal rank of SOS methods varied geographically, as did the relationships between SOS estimates and the cryospheric/hydrologic metrics. Compared with ground observations, SOS estimates were more related to the first leaf and first flowers expanding phenological stages. We found no evidence for time trends in spring arrival from ground- or model-based data; using an ensemble estimate from two methods that were more closely related to ground observations than other methods, SOS trends could be detected for only 12% of North America and were divided between trends towards both earlier and later spring.

Global Change Biology↗

Trace fossils and sedimentary facies from a Late Cambrian‐Early Ordovician tide‐dominated shelf (Santa Rosita Formation, northwest Argentina): Implications for ichnofacies models of shallow marine successions

The Santa Rosita Formation is one the most widely distributed lower Paleozoic units of northwest Argentina. At the Quebrada del Salto Alto section, east of Purmamarca, Jujuy Province, it is represented by four sedimentary facies: thick‐bedded planar cross‐stratified quartzose sandstones (A), thin‐bedded planar cross‐stratified quartzose sandstones and mudstones (B), wave‐rippled sandstones and bioturbated mudstones (C), and black and greenish gray shales (D). Paleocurrent data, sandstone architecture, and sedimentary structures from faciès A and B indicate bipolar/bimodal paleoflows, suggesting the action of tidal currents. The succession is interpreted as that of a tide‐dominated shelf, with only secondary influence of wave processes. Trace fossils are restricted to facies B and C. The Cruziana ichnocoenosis is preserved on the soles of thin‐bedded planar cross‐stratified quartzose sandstones (faciès B). This ichnocoenosis consists of Conostichus isp., Cruziana omanica, C. semiplicata, C. cf. tortworthi, Cruziana isp. Helminthopsis abeli, Monomorphichnus bilinearis, M. multilineatus, Palaeophycus tubularis, Rusophycus carbonarias, R. latus , and R. isp. The occurrence of Cruziana semiplicata , C. omanica, C. cf. tortworthi , and Rusophycus latus supports a Late Cambrian‐Tremadoc age. Slabbing of Cruziana shows complex interactions between biologic and sedimentologic processes, and suggests a predominance of exhumed traces, washed out and recast by tractive sand deposition. Sandstone soles are densely packed with biogenic structures and exhibit distinctive clusters of Rusophycus isp. that most likely represent trilobite nesting burrows. The Cruziana ichnocoenosis records the resident fauna of a protected, lower intertidal to subtidal interbar setting. The Skolithos ichnocoenosis is represented by high to low density vertical burrows of Skolithos linearis , which extend downwards to the quartzose sandstone soles of faciès B and cross the Cruziana ichnocoenosis. The Skolithos ichnocoenosis represents colonization by suspension‐feeding organisms following a major change in environmental conditions, related to the migration of lower intertidal to subtidal sandwaves. The Planolites ichnocoenosis consists exclusively of Planolites montanus within mudstones overlying wave‐rippled sandstones (facies C). The Planolites ichnocoenosis records opportunistic colonization by inf aunal deposit feeders that mined the organic‐rich fine‐grained sediment during the waning phase of storms that scoured organic detritus from the sea bottom. The section records, from base to top, a Cruziana‐Skolithos ichnofacies zone, a Skolithos ichnofacies zone and an unbioturbated zone typified by the thick‐bedded cross‐stratified quartzose sandstone (fades A). This trend reflects progressively higher energy conditions linked to the establishment of a large sand wave complex. The presence of a mixed Cruziana‐Skolithos ichnofacies in the lower interval reflects changes in substrate and energy levels, rather than water depth. Accordingly, contrasting ichnocoenoses from interbars (Cruziana) and sandwaves (Skolithos) must be considered an example of ichnofacies controlled by local parameters instead of general bathymétrie trends. Conversely, the vertical replacement of the Cruziana ichnofacies by the Skolithos ichnofacies towards the middle interval of the section reflects the environmental changes associated with the transition between the intertidal and subtidal zones. As overall tidal energy increases from supratidal to subtidal settings, the Skolithos ichnofacies tends to occur seaward of the Cruziana ichnofacies in tide‐dominated shallow marine environments. Therefore, onshore‐offshore ichnofacies replacement in tide‐dominated shallow seas is opposite to that in wave‐dominated marine settings.

Ichnos: An International Journal for Plant and Ani↗

Assessing spatial variability of nutrients, phytoplankton, and related water-quality constituents in the California Sacramento–San Joaquin Delta at the landscape scale—Comparison of four (2018, 2020, 2021, 2022) spring high-resolution mapping surveys

Executive Summary This report summarizes results from boat-based, high-resolution water-quality mapping surveys completed before, during, and after upgrades to the EchoWater Resource Recovery Facility (EchoWater Facility), the regional wastewater facility for the City of Sacramento and surrounding areas, near Elk Grove, California. Surveys were completed in the tidal aquatic environments of the Sacramento–San Joaquin Delta (Delta) in spring (May or June) 2018, 2020, 2021, and 2022. In each survey, a suite of in situ sensors were used to continuously (one measurement per second) measure water-quality conditions, nutrients, phytoplankton abundance, and species composition. In addition to in situ data collection, discrete water samples were collected about every 2 miles while underway for determination of phosphate, ammonium, and nitrate concentration. The boat stopped at about 30 locations to collect discrete samples for a suite of additional analytes, including phytoplankton enumeration. The four surveys represent snapshots in time across different phases of the EchoWater Facility Biological Nutrient Reduction (BNR) upgrade. The May 2018 survey represents conditions before the upgrade. The second survey (June 2020) represents conditions after implementation of the Nitrifying Sidestream Treatment. The third survey (May 2021) was completed immediately after the completion of the BNR upgrade and represents a transitional period, and the final survey (May 2022) represents post-upgrade conditions. Relevant hydrologic and climatic context such as water-year type, X 2 position (the distance from the Golden Gate Bridge to the point upstream where bottom salinity is 2 parts per thousand; Jassby and others, 1995), water export to import ratio, and management actions like the Delta Cross Channel gate operations are presented for each survey so they may be considered in comparisons among surveys. Differences in water-quality parameters, like turbidity, temperature, salinity, pH, and dissolved oxygen (DO) improve understanding of nutrient cycling and phytoplankton dynamics. Because the Delta is a complex system, we divided the study area into hydrologic zones to better examine general trends and obtain a broadscale view of differences among the 4 study years. Results are presented for each survey and parameter using box plots to compare the different hydrologic zones. We also present each parameter using contour maps by survey to display gradients across the system. The most evident change to water quality in the Delta across surveys is related to the EchoWater Facility BNR upgrade, which included nitrification and denitrification processes. Through this upgrade, effluent ammonium (NH 4 + ) concentrations were reduced by more than 95 percent (from about 2,000 micromolars [μM] to below the reporting limit of 35 μM), and nitrate (NO 3 − ) concentrations increased from near zero to about 500 μM; therefore, the concentration of dissolved inorganic nitrogen (DIN; the sum of NH 4 + and NO 3 − ) in the effluent was reduced by about 75 percent between May 2018 and May 2022. The BNR upgrade resulted in a reduction in NH 4 + concentrations in aquatic habitats immediately below the facility, designated as the “north Delta tidal transition zone” (Bergamaschi and others, 2024), from about 30 μM pre-upgrade to near zero during the 2022 spring survey, whereas effluent NO 3 − increased from median concentrations of about 7 μM to about 15 μM. Because of the reduced effluent nitrogen loads and variability in Sacramento River nitrogen loads from upstream sources, DIN concentrations in the north Delta tidal transition zone decreased from a median of 53.3 μM in 2018 to 35.3 μM in 2020, 20.7 μM in 2021, and 11.3 μM in 2022 during the spring surveys. The changes in DIN concentration and form observed in the north Delta tidal transition zone after the EchoWater Facility upgrade extended downstream but were rapidly altered by hydrologic mixing, biogeochemical processes, and other nutrient source inputs. Most of the Delta indicated near-zero concentrations of NH 4 + 1 year after the completion of the EchoWater Facility upgrades represented by the 2022 survey. Exceptions to this finding were observed in the San Joaquin River near Stockton and in Suisun Bay, indicating there are NH 4 + inputs to these locations from other sources (for example, Stockton Regional Wastewater Control Facility and Central Contra Costs Sanitary District wastewater treatment plants or agricultural and urban runoff). Although there was an increase in NO 3 − concentrations in the north Delta tidal transition zone after the upgrade, increases in NO 3 − in other zones were not apparent, presumably because nitrification of effluent derived ammonium was no longer a source of NO 3 − . Concentrations of DIN in many Delta zones were lower in 2022 compared to 2018 and 2020, with concentrations near or below what is considered potentially nitrogen limiting conditions for phytoplankton growth in the North Delta tidal transition zone and the Cache Slough complex channel system. Unrelated to the EchoWater Facility upgrade, NO 3 − and therefore DIN concentrations increased in the San Joaquin River near Stockton and in adjacent water bodies by survey date (likely associated with increasing drought conditions). The Mokelumne River had low DIN concentrations, except in 2018 when the Delta Cross Channel was open, which allowed nutrient-rich Sacramento River water to flow into this section of the river. Data from these surveys also support the hypothesis that nutrient drawdown during phytoplankton blooms may create localized nitrogen limiting conditions. The BNR upgrade resulted in lower effluent phosphate (PO 4 3− ) concentrations, which lowered PO 4 3− concentrations in some zones of the Delta during the four spring surveys; however, PO 4 3− concentrations throughout the Delta remained above 0.3 μM, indicating that primary productivity was not limited by phosphorous availability. DIN and PO 4 3− decreased after the upgrade in many areas of the Delta, and the DIN to dissolved inorganic phosphorus (DIN:DIP) ratio remained similar to pre-upgrade conditions and was often below the Redfield Ratio of 16, indicating nitrogen is more likely to limit phytoplankton growth than phosphorous. Inputs of dissolved organic carbon (DOC) from the EchoWater Facility are a minor source of this constituent to the Delta, so the upgrade had little to no effect on DOC concentrations across the Delta. Because phytoplankton abundance and species composition in the Delta are shaped by multiple factors other than nutrients (for example, light availability, temperature, salinity, and predation), it is important to consider these factors (as well as long-term monitoring) in addition to the EchoWater Facility upgrade. Although phytoplankton populations were low across much of the Delta during the spring surveys, several localized phytoplankton blooms (defined here as greater than 15 micrograms per liter [μg/L] of chlorophyll) provide insight into conditions that may favor the growth of beneficial and harmful species.

Callifornia↗

Effects of elevated CO2 on fine root dynamics in a Mojave Desert community: A FACE study

Fine roots (??? 1mm diameter) are critical in plant water and nutrient absorption, and it is important to understand how rising atmospheric CO2 will affect them as part of terrestrial ecosystem responses to global change. This study's objective was to determine effects of elevated CO2 on production, mortality, and standing crops of fine root length over 2 years in a free-air CO2 enrichment (FACE) facility in the Mojave Desert of southern Nevada, USA. Three replicate 25m diameter FACE rings were maintained at ambient (??? 370 ??mol mol-1) and elevated CO2 (??? 550 ??mol mol-1) atmospheric concentrations. Twenty-eight minirhizotron tubes were placed in each ring to sample three microsite locations: evergreen Larrea shrubs, drought-deciduous Ambrosia shrubs, and along systematic community transects (primarily in shrub interspaces which account for ??? 85% of the area). Seasonal dynamics were similar for ambient and elevated CO2: fine root production peaked in April-June, with peak standing crop occurring about 1 month later, and peak mortality occurring during the hot summer months, with higher values for all three measures in a wet year compared with a dry year. Fine root standing crop, production, and mortality were not significantly different between treatments except standing crop along community transects, where fine root length was significantly lower in elevated CO2. Fine root turnover (annual cumulative mortality/mean standing crop) ranged from 2.33 to 3.17 year-1, and was not significantly different among CO2 treatments, except for community transect tubes where it was significantly lower for elevated CO2. There were no differences in fine root responses to CO2 between evergreen (Larrea) and drought-deciduous (Ambrosia) shrubs. Combined with observations of increased leaf-level water-use efficiency and lack of soil moisture differences, these results suggest that under elevated CO2 conditions, reduced root systems (compared with ambient CO2) appear sufficient to provide resources for modest aboveground production increases across the community, but in more fertile shrub microsites, fine root systems of comparable size with those in ambient CO2 were required to support the greater aboveground production increases. For community transects, development of the difference in fine root standing crops occurred primarily through lower stimulation of fine root production in the elevated CO2 treatment during periods of high water availability. ?? 2005 Blackwell Publishing Ltd.

Global Change Biology↗

Water quality in the Arthur R. Marshall Loxahatchee National Wildlife Refuge — Trends and spatial characteristics of selected constituents, 1974-2004

Water quality in the interior marsh of the Arthur R. Marshall Loxahatchee National Wildlife Refuge is characterized by low concentrations of major ions, principally sodium and chloride, and is affected primarily by natural seasonal processes, such as evapotranspiration, rainfall, and biological activity. During the dry season, evapotranspiration exceeds precipitation, and specific conductance and conservative ion concentrations at marsh background sites typically increase by 40-70 percent between the end of the rainy season in September and the end of the dry season in May. Water enters the Refuge mainly from rainfall and perimeter canals. Water is pumped into the perimeter canals from large pumping stations, such as S-5A and S-6. In recent years, much of the water pumped into the Refuge passes through Stormwater Treatment Areas (STAs) before being released into the perimeter canals that surround the Refuge. Since 2001, water at S-6 has been diverted south toward STA-2, away from the Refuge perimeter canals. Water from S-5A and S-6 flows through agricultural lands with intense agricultural activity and typically contains relatively high concentrations of major ions, nutrients, and pesticides. Specific conductance, major-ion concentrations, and nutrient concentrations are an order of magnitude higher at S-5A and S-6 canal sites than at interior marsh sites. Water quality in the marsh bordering the canals can be affected substantially by the canal water, and these effects can extend several miles or more into the marsh depending on location in the Refuge and on the water level in the canals. As canal water flows into the marsh, processes such as uptake by periphyton and rooted vegetation and settling of particulate matter reduce the concentrations of nutrients to a greater extent than conservative ions such as chloride. Long- and short-term trends for specific conductance, chloride ion, sulfate ion, total phosphorus, and total nitrogen at five sites were evaluated primarily using an uncensored seasonal Kendall test with a water-level adjustment to reduce the effects of long wet or dry periods. Significant long-term trends (1974-2003) for specific conductance, chloride, total phosphorus, and total nitrogen at canal sites S-5A and S-6 were generally downward. Of the five sites, S-5A had the most pronounced decline for specific conductance at about -340 microsiemens per centimeter (?S/cm), followed by S-6 with a decline of about -280 ?S/cm. The two internal marsh sites, LOX8 and LOX13, had significant long-term trends in specific conductance of about +37 and -36 ?S/cm, respectively. Long-term trends for other constituents at the two internal marsh sites were generally small in magnitude or not measurable between 1978 and 2003. Marsh site LOX15 near the Hillsboro Canal showed no long-term trends, although specific conductance and sulfate concentration increased about 560 ?S/cm and 30 milligrams per liter, respectively, from 1998 to 2002. Site LOX15 is influenced strongly by intrusions of canal water, and increases in specific conductance and sulfate at this site coincided with increased canal-water inflows from STA-1W between 2001 and 2003. Median concentrations at LOX13 and S-5A were used to represent background and canal concentrations, respectively. Based on these values, the median chloride concentration at LOX15 indicates that the water is typically about 31 percent canal water and 69 percent ?natural? background water. Using median sulfate concentrations, similarly to chloride, the fraction of water at LOX15 was estimated to be 17 percent from canals and 83 percent from ?natural? background water. This finding suggests that in the low sulfate environment of the Refuge, sulfate is not conservative and only about half of the sulfate from canal water typically reaches LOX15; the rest presumably is removed by marsh plants, algae, and bottom sediments. Concentrations of pesticides and other organic compounds were measured

Florida↗

Gore Creek watershed, Colorado — Assessment of historical and current water quantity, water quality, and aquatic ecology, 1968–98

The historical and current (1998) water-quantity, water-quality, and aquatic-ecology conditions in the Gore Creek watershed are described as part of a study by the U.S. Geological Survey, done in cooperation with the Town of Vail, the Eagle River Water and Sanitation District, and the Upper Eagle Regional Water Authority. Interpretation of the available water-quantity, water-quality, and aquatic-ecology data collected by various agencies since 1968 showed that background geology and land use in the watershed influence the water quality and stream biota. Surface-water nutrient concentrations generally increased as water moved downstream through the Town of Vail, but concentrations at the mouth of Gore Creek were typical when compared with national data for urban/undeveloped sites. Nitrate concentrations in Gore Creek were highest just downstream from a wastewater-treatment plant discharge, but concentrations decreased at sites farther downstream because of dilution and nitrogen uptake by algae. Recent total phosphorus concentrations were somewhat elevated when compared to the U.S. Environmental Protection Agency recommended level of 0.10 milligram per liter for control of eutrophication in flowing water. However, total phosphorus concentrations at the mouth of Gore Creek were relatively low when compared to a national study of phosphorus in urban land-use areas. Historically, suspended sediment associated with construction of Interstate 70 in the early 1970's has been of primary concern; however, recent data indicate that streambed aggradation of sediment originating from Interstate 70 traction sanding currently is a greater concern. About 4,000 tons of coarse sand and fine gravel is washed into Black Gore Creek each year following application of traction materials to Interstate 70 during adverse winter driving conditions. Suspended-sediment concentrations were low in Black Gore Creek; however, bedload-transport rates of as much as 4 tons per day have been measured. Water samples were collected during spring and fall of 1997 from five alluvial monitoring wells located throughout the Town of Vail. Nutrient concentrations generally were low in the alluvial monitoring wells. Specific-conductance values ranged from 265 to 557 microsiemens per centimeter at 25 degrees Celsius. Concentrations of radon in monitoring-well samples exceeded the 300-picocuries-per-liter U.S. Environmental Protection Agency proposed maximum contaminant level (which has been suspended pending further review). Low levels of bacteria and methylene blue active substances indicate there is little or no wastewater contamination of shallow ground water in the vicinity of the monitoring wells and one of the municipal water-supply wells. Ground-water ages in the alluvial aquifer ranged from about 2 to about 50 years old. These ages indicate that changes in land-management practices may not have an effect on ground-water quality for many years. Differences in macroinvertebrate-community structure were found among sites in Gore Creek by evaluating changes in relative abundance, total abundance, and dominant functional feeding groups of the major macroinvertebrate groups. Ephemeroptera (mayflies), Plecoptera (stoneflies), Trichoptera (caddisflies), and Coleoptera (beetles) exhibited relatively low tolerance to water-quality degradation when compared with Diptera (midges) and non-insects (sludge worms). More than 80 percent of the macroinvertebrate community at sites located farthest upstream was composed of mayflies, stoneflies, and caddisflies, indicating favorable water-quality and habitat conditions. The relative percentages of midges and sludge worms greatly increased in the downstream reaches of Gore Creek, which drain relatively larger areas of urban and recreation land uses, indicating the occurrence of nutrient and organic enrichment in Gore Creek. The macroinvertebrate community in Black Gore Creek indicated adverse effects from sediment deposition. Macroinvertebrate abundance was considerably reduced at the two sites where streambed sediment was more prevalent; however, differences in abundance also may have been related to differences in habitat and availability of food resources. The lower 4 miles of Gore Creek, downstream from Red Sandstone Creek, have been designated a Gold Medal fishery in recognition of the high recreational value of the abundant brown trout community. Gore Creek contained twice as many trout as a reference site with similar habitat characteristics in Rocky Mountain National Park. Moderate increases in nutrient concentrations above background conditions have increased the growth and abundance potential for aquatic life in Gore Creek, while at the same time, esthetic and water-quality conditions have remained favorable. The spatial distribution of nitrate concentrations was consistent with the observed spatial distribution of algal biomass and macroinvertebrate-community characteristics. Algal biomass was limited by available resources (sunlight and nutrients) in the upstream reaches of Gore Creek and limited by macroinvertebrate grazing and water-quality conditions in the downstream reaches. The fish community has benefited from enhanced biological production in the downstream reach of Gore Creek. Increases in algal biomass and macroinvertebrate abundance, in response to higher nutrient concentrations, provide ample food resources necessary to support the abundant fish community. Trace-element data for surface water, ground water, streambed sediment, fish tissue, and macroinvertebrate tissue indicate that concentrations are generally low in the Gore Creek watershed. In streambed-sediment samples, cadmium, copper, and zinc concentrations were below background levels reported for the Upper Colorado River Basin in Colorado. Concentrations of cadmium, copper, iron, and silver in surface water have occasionally exceeded stream standards in the past, but recent surface-water data indicate these trace elements currently are not of concern. Manganese concentrations commonly exceeded the 50-microgram-per-liter stream standard in Black Gore Creek. Elevated manganese concentrations were primarily attributable to the sedimentary geology of the area. Concentrations of organic constituents are low in the Gore Creek watershed. Pesticides were detected infrequently and at low concentrations in surface-water, ground-water, bed-sediment, and whole-body fish-tissue samples. Volatile organic compounds also were detected at low concentrations in surface- and ground-water samples.

Colorado↗

Temperature and winter duration requirements for reproductive success in johnny darter Etheostoma nigrum in the South Platte River basin, Colorado

Changes in water temperature and its seasonal timing influences the physiological processes of many aquatic ectotherms. Wastewater treatment plants (WWTP) contribute to warmer and more consistent water temperatures in streams draining the North American Great Plains, particularly during winter months. Reduced variation in seasonal water temperature may adversely affect fishes that rely on temperature fluctuation or specific overwintering temperatures for reproductive cues. We used johnny darter Etheostoma nigrum (Family Percidae), a thermally sensitive species native to the South Platte River Basin, as a focal species to assess the effects of winter water temperature regulations in Colorado. Our objective was to evaluate the effects of winter stream temperature and winter duration on reproductive success of johnny darter in the laboratory to inform development of water quality criteria for the South Platte River Basin. Winter duration (60, 90 or 120 days) and temperature (4 or 12°C) were crossed in a factorial design of six treatments that simulated warmed effluent-impacted streams as well as streams with a more natural thermal regime. We defined reproductive success in johnny darter by examining four reproductive endpoints: (1) spawning timing, (2) egg and larvae production, (3) egg success, and (4) egg development. Across the four endpoints, seven main response variables were measured: spawning initiation date, eggs per gram of female per week, live larvae per gram of female per week, fertilisation success, hatching success, egg-to-larva survival and days to hatch. Winter temperature and duration influenced spawning timing and egg development. Earlier spawning initiation dates were observed in fish exposed to 12°C winters and was associated with a higher number of days to hatch. Eggs per gram of female per week, live larvae per gram of female per week, fertilisation success, hatching success, and egg-to-larva survival were similar among winter treatments. Although the State of Colorado chronic winter water temperature standard of 12°C appears to be adequate for egg and larvae production in johnny darter, our results showed that fish exposed to the standard began spawning significantly earlier than fish exposed to 4°C winters. Evaluating reproductive output in the context of seasonal timing is valid because spawning timing has the potential to affect overall production and egg development, and consequently recruitment and population sustainability. Therefore, spawning timing should be considered when making management decisions and revising water temperature standards. The warming of streams and rivers from WWTP effluent is a global issue, especially in areas that are undergoing rapid urbanisation. Fishes are particularly sensitive to water temperature and many fishes worldwide are likely to be experiencing changes in important physiological processes, affecting both reproduction and survival. Our study indicates that reproductive timing could be influenced by stream warming and stresses the need for further studies on the influence of anthropogenic effects on stream warming.

Colorado↗

Managing effects of drought and other water resource challenges in Alaska and the Pacific Northwest

This is a Cooperator Report. As such, there is no specific abstract. The physical, ecological, and social environments of Alaska and the Pacific Northwest (PNW) region of the United States are extremely diverse. Alaska ranges from the Arctic Ocean and the very cold, dry environments of the North Slope to the cool and very rainy coastal North Pacific region of Southeast Alaska. Most precipitation falls as snow at higher elevations. In Arctic Alaska, average annual temperature is 14.6 F, and average annual precipitation is 11 inches. By contrast, in Southeast Alaska, average annual temperature is 35.8 F, and annual average precipitation is 143 inches. The PNW, defined here as Idaho, Oregon, and Washington, ranges from the Pacific Coast (annual precipitation of 200 inches) to interior semi-arid regions (annual precipitation of 8 inches). Precipitation patterns in the PNW are strongly governed by orographic phenomena, with high, persistent snowpack in the higher mountains (e.g., record annual snowfall of 1,130 in at Mount Baker, Washington in 1999-2000). Ecosystems in the PNW include productive temperate coniferous forests near the Pacific coast and along the (wet) west slope of the Cascade Range, less productive mixed-conifer forest along the (dry) east slope of the Cascades and in interior mountain ranges, and sagebrush-steppe and shrublands at lower elevations in much of the interior and mountain valleys. Large rivers and thousands of smaller tributaries form an extensive network of riparian, wetland, and estuarine systems that provide both critical hydrologic function and biological diversity at broad and fine spatial scales. Although Alaska and the Pacific Northwest differ in important physical, ecological, and social features, the importance of natural resources is evident in both regions. Water is important for wildlife and people. Water provides critical habitat for salmon, which are culturally and economically valuable species. Timber production has declined in recent decades. Recreation has emerged as a major revenue source.

Alaska, Oregon, Washington↗

Mechanisms for retention of low molecular weight organic carbon varies with soil depth at a coastal prairie ecosystem

Though primary sources of carbon (C) to soil are plant inputs (e.g., rhizodeposits), the role of microorganisms as mediators of soil organic carbon (SOC) retention is increasingly recognized. Yet, insufficient knowledge of sub-soil processes complicates attempts to describe microbial-driven C cycling at depth as most studies of microbial-mineral-C interactions focus on surface horizons. We leveraged a well-studied paleo-marine terrace (90 ka) located near Santa Cruz, CA, to characterize the short-term (days to weeks) and intermediate-term (months to years) fate of two low molecular weight organic carbon. compounds at three depths in the soil profile (∼25 cm, A horizon; ∼75 cm A/B transition; and ∼125 cm, B horizon). We employed isotopically-labeled glucose (GLU) and oxalic acid (OXA) to represent two common classes of rhizodeposits: carbohydrates and organic acids. Using a combination of laboratory (9 d) and field (490 d) incubations, we traced the fate of GLU-C and OXA-C through dissolved-, metal-associated-, and microbially-respired CO2 and bulk SOC pools. Our results suggest new SOC retention (i.e., defined as 13C label identified in solid or aqueous fractions) over intermediate time frames (490 d) is correlated with patterns in short-term (9 d) cycling dynamics, which in turn is related to the theoretical efficiency by which microorganisms process each substrate. For all horizons (A, A/B, and B) GLU-C was converted to CO2 more quickly than OXA-C with modeled decomposition rates ∼2–4 times faster for GLU depending on microbial density (higher in A than B horizon). The faster decomposition rates of GLU-C increased fractional recovery (0.399 ± 0.026 to 0.504 ± 0.030 for GLU-C) compared to OXA-C (0.035 ± 0.003 to 0.127 ± 0.010) among all horizons in our field experiment (490 d). Though the overall proportion of GLU-C recovered in solid fractions did not vary significantly with horizon, based on 13C recovered in aqueous fractions the apparent mechanism for retention did. After the 9-d laboratory incubation, fractional recovery for GLU-C among C pools associated with microbial biomass was almost 20× higher than OXA-C (0.192 versus 0.010, respectively across all horizons). More than a year later, 43–46% of GLU-C retained in the field incubation was extractable with a neutral salt (representing a pool of soil C residing within or available to microbial biomass) among A and A/B horizons, while only 6% of retained GLU-C was similarly extractable in the B horizon. Thus, it appears among depths with higher microbial density (A, A/B horizons), anabolic recycling is the most likely process contributing to the persistence of glucose C, whereas abiotic sinks contributed more to intermediate-term stability for GLU-C in the B horizon. By contrast, most OXA-C was lost, presumably as CO2, over the short-term from the A and A/B horizons (fractional recovery: 0.136 ± 0.011 and 0.091 ± 0.002, respectively). However, though substantially lower than GLU-C recovered at the conclusion of our field experiment, the fraction of oxalic acid C retained in the B horizon over both short- (0.72 ± 0.037) and intermediate-time (0.127 ± 0.010) frames was several-fold higher than for overlying horizons. The specific process(es) (e.g., more efficient microbial utilization, metal-organic complexation, direct adsorption to the mineral matrix, etc.) contributing to higher retention for OXA-C at depth are discussed but remain unresolved.

Soil Biology and Biochemistry↗