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Real-time piscicide tracking using Rhodamine WT dye for support of application, transport, and deactivation strategies in riverine environments

Piscicide applications in riverine environments are complicated by the advection and dispersion of the piscicide by the flowing water. Proper deactivation of the fish toxin is required outside of the treatment reach to ensure that there is minimal collateral damage to fisheries downstream or in connecting and adjacent water bodies. In urban settings and highly managed waterways, further complications arise from the influence of industrial intakes and outfalls, stormwater outfalls, lock and dam operations, and general unsteady flow conditions. These complications affect the local hydrodynamics and ultimately the transport and fate of the piscicide. This report presents two techniques using Rhodamine WT dye for real-time tracking of a piscicide plume—or any passive contaminant—in rivers and waterways in natural and urban settings. Passive contaminants are those that are present in such low concentration that there is no effect (such as buoyancy) on the fluid dynamics of the receiving water body. These methods, when combined with data logging and archiving, allow for visualization and documentation of the application and deactivation process. Real-time tracking and documentation of rotenone applications in rivers and urban waterways was accomplished by encasing the rotenone plume in a plume of Rhodamine WT dye and using vessel-mounted submersible fluorometers together with acoustic Doppler current profilers (ADCP) and global positioning system (GPS) receivers to track the dye and map the water currents responsible for advection and dispersion. In this study, two methods were used to track rotenone plumes: (1) simultaneous injection of dye with rotenone and (2) delineation of the upstream and downstream boundaries of the treatment zone with dye. All data were logged and displayed on a shipboard laptop computer, so that survey personnel provided real-time feedback about the extent of the rotenone plume to rotenone application and deactivation personnel. Further, these strategies facilitate adjustment of rotenone application and deactivation strategies in real time if necessary based on the observed advection and dispersion of the rotenone plume. Two large-scale and complex applications of rotenone in the Chicago Area Waterway System (CAWS) in 2009 and 2010 to combat invasive Asian carp are documented in this report. The application in Chicago Sanitary and Ship Canal (CSSC) in December 2009 involved more than 1,800 gallons of rotenone injected at multiple stations through a 6.2-mile reach of the canal near Lockport, Illinois. The rotenone plume was encased in Rhodamine WT dye so that two survey boats provided real-time feedback to shore personnel regarding the plume extent as it advected downstream. Real-time tracking of the rotenone was essential in this large-scale application because of the multistage injection strategy and the numerous deactivation points required to minimize collateral damage to fisheries in surrounding and receiving water bodies. All timing of application and deactivation operations relied on dye tracking. A second application of rotenone in May 2010 to the Little Calumet River near O’Brien Lock and Dam (Illinois) provided another opportunity for dye-tracking support operations; however, application and deactivation strategies were designed considering zero-flow conditions within the reach of interest. Therefore, dye was injected at the upstream and downstream boundaries of the rotenone application reach and was used to track movement of water in and out of a treatment reach, allowing proper deactivation to occur and avoiding unnecessary damage to fisheries downstream. The data collected during the real-time tracking operations for both applications allowed full documentation of the rotenone treatment for archival purposes and provided information for future applications. The methods presented in this report for real-time tracking and documentation of piscicide applications in riverine environments worked exceptionally well and allowed the multiagency Asian Carp Rapid Response Workgroup to carry out large-scale rotenone applications in urban waterways in an environmentally responsible manner with minimal collateral damage to fisheries outside the treatment reach. Traveltime information extracted from the boat-mounted and fixed-position fluorometers agrees well with empirical predictions from a preliminary dye study (mock rotenone injection) on this system completed in November 2009 on the CSSC and with previously published methods for estimating traveltimes of the peak, leading edge, and trailing edge of the plume. Although the rotenone application strategy called for zero-flow conditions on the Little Calumet River in 2010, downstream advection of treated water did occur, and dye tracing combined with velocity mapping allowed this advection to be documented and exposed the unique hydrodynamics and mixing within this reach. The large volumes of data collected during the operations allow documentation and visualization of the rotenone applications, thus providing feedback to planners and archival of the treatments for future reference. The methods developed in this report are directly transferrable to piscicide applications in water bodies in other locations, including rivers, ponds, or lakes, and can be used for real-time tracking of any passive contaminant that may enter a water body.

Illinois↗

Natural resource condition assessment: Channel Islands National Park

The Natural Resource Condition Assessment (NRCA) Program aims to provide documentation about the current conditions of important Park natural resources through a spatially explicit, multidisciplinary synthesis of existing scientific data and knowledge. Findings from the NRCA will help Channel Islands National Park (CINP) managers to develop near-term management priorities. The central objectives of this assessment were to evaluate the current conditions and trends of key Park resources, identify important knowledge gaps, and determine key stressors and threats. Because a recent report provided an assessment of marine resources (Engle 2006), which constitute 50% of the park, this NRCA focused on terrestrial resources. For this NRCA, CINP resource managers identified that the greatest Park need was to know whether or not vegetation (primarily woody vegetation) is recovering on the Channel Islands and how this may be influencing recovery of the native terrestrial vertebrates. To address this, the CINP NRCA team evaluated the condition of island vegetation and vertebrate communities using both: 1) already available, summarized information from reports, theses, and journal articles and 2) new analyses of recently available, existing data collected on the islands. Descriptions of specific methods used to analyze available data by the NRCA team are provided within the assessment sections in Chapter 4 of this report. The Team used the Vital Signs indicators as identified by Fancy et al. (2009) for monitoring the condition of Natural Resources in U.S. National Parks. Using this framework, the focal study resource components were the five CINP islands, because these are the bio-geophysical resources of greatest interest to the CINP. Each of the islands has its own unique history of human impacts, ecological conditions, restoration efforts, and natural resource monitoring. The Team evaluated island scrub recovery by assessing changes over time in two metrics: 1) vegetation community structure (species composition, cover, and spatial extent), and 2) vertebrate abundance, relative to the reference condition of each island. The reference conditions for this NRCA were the particular island’s ecological state prior to European settlement or ranching, which represent the ecological state to which the NPS desires the park return. For each metric, the Team determined the Reference Conditions/Values, Current Condition, Trend, and Recovery Potential (based on a review of the literature and from the NRCA team’s analysis of newly available data), Threats and Stressors, Data Gaps, Overall Condition. A qualitative statement of overall current condition was created for each metric, after all data and literature relevant to the measures (i.e., vegetation and vertebrates) of each resource component (i.e., island) were reviewed and evaluated. All information gathered for this NRCA is available and managed by CINP, so that the compilation of resources would be available not only for the assessment team, but will be provided for future Park reference. Chapter 1 of this NRCA provides a descriptive background of NRCAs. Chapter 2 provides Park-wide natural resource background information, while Chapter 3 describes the overall methodological approach used in this assessment. Chapter 4 provides a detailed assessment of each island’s condition, which is then summarized Park-wide and for each island in Chapter 5. Based on both vegetation recovery and vertebrate population abundances, our assessment found Santa Cruz Island (SCI), Santa Rosa Island (SRI), San Miguel Island (SMI), and Anacapa Island (AI) to be, overall, in moderate condition and slowly recovering following the removal of non-native herbivores. The condition of Santa Barbara Island (SBI) is poor and remains in a state that is not likely to improve without assisted recovery by the Park. All islands have exhibited some level of native vegetation recovery, with most of the observed natural recovery occurring on SCI, SRI, SMI, and AI; very little recovery has occurred at present on SBI. Vegetation recovery is uneven across each island and largely limited to mesic areas, such as north-facing slopes. Areas that will likely require some assisted recovery efforts are those that are: at risk of erosion, isolated, lacking native seed banks, and plagued with high competition of non-native grasses and other invasives. Some plant communities, such as exposed upland vegetation on SRI, also have lower natural recovery potential and will likely require assisted recovery. The overall condition of the Park’s terrestrial island vertebrate species generally appears to be good and improving, with high potential for further recovery to reach pre-ranching era reference conditions. This is particularly true for vertebrates on SCI, SRI, and SMI. Although there are limited data for AI, the data that are available suggest that vertebrates on that island are in good condition and improving as well. However, the condition of SBI vertebrates is only moderate, with natural recovery potential being low, with many landbird species’ populations currently declining, extinct, or not improving. An overarching theme across the Park’s terrestrial island vertebrate populations is that many of them are in good condition because the particular animals are habitat generalists, so their population dynamics are not strongly tied to habitat conditions. However, those landbirds associated with riparian vegetation are not showing signs of recovery across the Park islands, and riparian areas appear to be one of the habitats with limited natural recovery potential on the islands. Overwhelmingly, the most critical data gap that emerged from our analysis was the lack of current vegetation maps for most of the islands (AI, SCI, SRI, SMI). The lack of this information made it difficult to assess spatial changes in island vegetation over time, and to relate those changes to observed changes in vertebrate species abundances. Therefore, we were limited in our spatial assessment of vegetation recovery for AI, SCI, SRI, and SMI, and were only able to relate the current vegetation with island landbird analysis for SBI. Another common theme across islands was the lack of information on best techniques for shrub reintroduction and recovery, and the success of invasive species removal and re-vegetation efforts. Abundance, distribution, and habitat affinities of reptiles and amphibians are poorly known for AI, SCI, SRI, and SMI, making it difficult to assess their population condition and trends, and their potential responses to shrub recovery. Mammal and landbird populations are reasonably well-sampled across most of the islands, but data are lacking for most vertebrates from AI, especially West and Middle AI where no vertebrate sampling occurs. The population status, cause of declines, and recovery needs are lacking for several landbird species across multiple islands, including loggerhead shrikes ( Lanius ludovicianus ssp.) on SCI and SRI, and Santa Cruz Island rufous crowned sparrows ( Aimophila ruficeps obscura ) on AI. Additionally, island song sparrows ( Melospiza melodia graminea ) remain extirpated on SBI and data are needed for assessing their recovery potential on the island. The potentially major threats facing the islands’ flora and fauna today are: areas of continued erosion, potential new non-native introductions, and climate change. The Park islands have already experienced warming over the last several decades due to human-induced climate change, and are projected to experience continued warming. These increasing stresses imposed by climate change may slow and inhibit natural recovery potentials for island fauna, and especially flora.

California↗

Land surface phenology

Certain vegetation types (e.g., deciduous shrubs, deciduous trees, grasslands) have distinct life cycles marked by the growth and senescence of leaves and periods of enhanced photosynthetic activity. Where these types exist, recurring changes in foliage alter the reflectance of electromagnetic radiation from the land surface, which can be measured using remote sensors. The timing of these recurring changes in reflectance is called land surface phenology (LSP). During recent decades, a variety of methods have been used to derive LSP metrics from time series of reflectance measurements acquired by satellite-borne sensors. In contrast to conventional phenology observations, LSP metrics represent the timing of reflectance changes that are driven by the aggregate activity of vegetation within the areal unit measured by the satellite sensor and do not directly provide information about the phenology of individual plants, species, or their phenophases. Despite the generalized nature of satellite sensor-derived measurements, they have proven useful for studying changes in LSP associated with various phenomena. This chapter provides a detailed overview of the use of satellite remote sensing to monitor LSP. First, the theoretical basis for the application of satellite remote sensing to the study of vegetation phenology is presented. After establishing a theoretical foundation for LSP, methods of deriving and validating LSP metrics are discussed. This chapter concludes with a discussion of major research findings and current and future research directions.

Book chapter↗

Quantifying anthropogenic contributions to century-scale groundwater salinity changes, San Joaquin Valley, California, USA

Total dissolved solids (TDS) concentrations in groundwater tapped for beneficial uses (drinking water, irrigation, freshwater industrial) have increased on average by about 100 mg/L over the last 100 years in the San Joaquin Valley, California (SJV). During this period land use in the SJV changed from natural vegetation and dryland agriculture to dominantly irrigated agriculture with growing urban areas. Century-scale salinity trends were evaluated by comparing TDS concentrations and major ion compositions of groundwater from wells sampled in 1910 (Historic) to data from wells sampled in 1993-2015 (Modern). TDS concentrations in subregions of the SJV, the southern (SSJV), western (WSJV), northeastern (NESJV), and southeastern (SESJV) were calculated using a cell-declustering method. TDS concentrations increased in all regions, with the greatest increases found in the SSJV and SESJV. Evaluation of the Modern data from the NESJV and SESJV found higher TDS concentrations in recently recharged (post-1950) groundwater from shallow (< 50 m) wells surrounded predominantly by agricultural land uses, while premodern (pre-1950) groundwater from deeper wells, and recently recharged groundwater from wells surrounded by mainly urban, natural, and mixed land uses had lower TDS concentrations, approaching the TDS concentrations in the Historic groundwater. For the NESJV and SESJV, inverse geochemical modeling with PHREEQC indicated that weathering of primary silicate minerals accounted for the majority of the increase in TDS concentrations, contributing more than nitrate from fertilizers and sulfate from soil amendments combined. Bicarbonate showed the greatest increase among major ions, resulting from enhanced silicate weathering due to recharge of irrigation water enriched in CO2 during the growing season. The results of this study demonstrate that large anthropogenic changes to the hydrologic regime, like massive development of irrigated agriculture in semi-arid areas like the SJV, can cause large changes in groundwater quality on a regional scale.

California↗

Valuation of rangeland ecosystem services

Economic valuation lends itself well to the anthropocentric orientation of ecosystem services. An economic perspective on ecosystems portrays them as natural assets providing a flow of goods and services valuable to individuals and society collectively. A few examples include the purification of drinking water, reduced risk from flooding and other extreme events, pollination of agricultural crops, climate regulation, and recreation opportunities from plant and animal habitat maintenance, among many others. Once these goods and services are identified and quantified, they can be monetized to complete the valuation process. The monetization of ecosystem goods and services (in the form of dollars) provides a common metric that allows for cross-comparison of attributes and evaluation of differing ecological scenarios. Complicating the monetization process is the fact that most of these goods and services are public and non-market in nature; meaning they are non-rival and non-exclusive and are typically not sold in a traditional market setting where monetary values are revealed. Instead, one must employ non-market valuation techniques, with primary valuation methods typically being very time and resource consuming, intimidating to non-economists, and often impractical. For these reasons, benefit transfer methods have gained popularity. This methodology harnesses the primary collection results of existing studies to make inferences about the economic values of non-market goods and services at an alternative policy site (in place and/or in time). For instance, if a primary valuation study on oak reestablishment on rangelands in southern California yielded a value of $30 per-acre associated with water regulation, this result can be transferred, with some adjustments, to say something about the value of an acre of oaks on rangelands in northern portions of the state. The economic valuation of rangeland ecosystem services has many roles. Economic values may be used as input into analyzing the costs and benefits associated with policies being proposed, or possibly already implemented. For example, with monetized values acting as a common metric, one could compare the 'benefits' of converting a rangeland ecosystem for commercial development (perhaps estimated at the market value of the developed land) with the foregone ecosystem service values (in addition to any land income lost) resulting from that land conversion. Similarly, ecosystem service values can be used to determine the level of return on an investment. rhis is a primary objective for private land conservation organizations who typically have very limited resources. Ecosystem service valuation can also have a role in damage assessments from incidents that require compensation such as oil spills. Additionally, valuation can be very informative when investigating regulatory programs that trade ecological assets such as wetland mitigation programs. Typically these programs are based simply on an 'acre for acre' criterion, and do not take into consideration varying welfare values associated with that ecosystem. Lastly, and most fundamental, ecosystem service valuation serves as a recognition tool for people of all backgrounds. Identifying and valuing ecosystem goods and services on rangelands brings to light the value these natural assets have to human welfare that often remain hidden do to their public and non-market attributes. This type of recognition is vital to the preservation of rangeland ecosystems in the future and the many ecological benefits they provide.

Conference Paper↗

Can you hear me now? Range-testing a submerged passive acoustic receiver array in a Caribbean coral reef habitat

Submerged passive acoustic technology allows researchers to investigate spatial and temporal movement patterns of many marine and freshwater species. The technology uses receivers to detect and record acoustic transmissions emitted from tags attached to an individual. Acoustic signal strength naturally attenuates over distance, but numerous environmental variables also affect the probability a tag is detected. Knowledge of receiver range is crucial for designing acoustic arrays and analyzing telemetry data. Here, we present a method for testing a relatively large-scale receiver array in a dynamic Caribbean coastal environment intended for long-term monitoring of multiple species. The U.S. Geological Survey and several academic institutions in collaboration with resource management at Buck Island Reef National Monument (BIRNM), off the coast of St. Croix, recently deployed a 52 passive acoustic receiver array. We targeted 19 array-representative receivers for range-testing by submersing fixed delay interval range-testing tags at various distance intervals in each cardinal direction from a receiver for a minimum of an hour. Using a generalized linear mixed model (GLMM), we estimated the probability of detection across the array and assessed the effect of water depth, habitat, wind, temperature, and time of day on the probability of detection. The predicted probability of detection across the entire array at 100 m distance from a receiver was 58.2% (95% CI: 44.0&ndash;73.0%) and dropped to 26.0% (95% CI: 11.4&ndash;39.3%) 200 m from a receiver indicating a somewhat constrained effective detection range. Detection probability varied across habitat classes with the greatest effective detection range occurring in homogenous sand substrate and the smallest in high rugosity reef. Predicted probability of detection across BIRNM highlights potential gaps in coverage using the current array as well as limitations of passive acoustic technology within a complex coral reef environment.

Ecology and Evolution↗

Development and validation of a spatially-explicit agent-based model for space utilization by African savanna elephants (Loxodonta africana) based on determinants of movement

African elephants (Loxodonta africana) are well-studied and inhabit diverse landscapes that are being transformed by both humans and natural forces. Most tools currently in use are limited in their ability to predict how elephants will respond to novel changes in the environment. Individual-, or agent-based modeling (ABM), may extend current methods in addressing and predicting spatial responses to environmental conditions over time. We developed a spatially explicit agent-based model to simulate elephant space use and validated the model with movement data from elephants in Kruger National Park (KNP) and Chobe National Park (CNP). We simulated movement at an hourly scale, as this scale can reflect switches in elephant behavior due to changes in internal states and short-term responses to the local availability and distribution of critical resources, including forage, water, and shade. Known internal drivers of elephant movement, including perceived temperature and the time since an individual last visited a water source, were linked to the external environment through behavior-based movement rules. Simulations were run on model landscapes representing the wet season and the hot, dry season for both parks. The model outputs, including home range size, daily displacement distance, net displacement distance, and maximum distance traveled from a permanent water source, were evaluated through qualitative and quantitative comparisons to actual elephant movement data from both KNP and CNP. The ABM was successful in reproducing the differences in daily displacements between seasons in each park, and in distances traveled from a permanent water source between parks and seasons. Other movement characteristics, including differences in home range sizes and net daily displacements, were partially reproduced. Out of the all the statistical comparisons made between the empirical and simulated movement patterns, the majority were classified as discrepancies of medium or small effect size. We have shown that a resource-driven model with relatively simple decision rules generates trajectories with movement characteristics that are mostly comparable to those calculated from empirical data. Simulating hourly movement (as our model does) may be useful in predicting how finer-scale patterns of space use, such as those created by foraging movements, are influenced by finer spatio-temporal changes in the environment.

Chobe National Park, Kruger National Park↗

A comparison of surface water natural organic matter in raw filtered water samples, XAD, and reverse osmosis isolates

This research compared raw filtered waters (RFWs), XAD resin isolates (XAD-8 and XAD-4), and reverse osmosis (RO) isolates of several surface water samples from McDonalds Branch, a small freshwater fen in the New Jersey Pine Barrens (USA). RO and XAD-8 are two of the most common techniques used to isolate natural organic matter (NOM) for studies of composition and reactivity; therefore, it is important to understand how the isolates differ from bulk (unisolated) samples and from one another. Although, any comparison between the isolation methods needs to consider that XAD-8 is specifically designed to isolate the humic fraction, whereas RO concentrates a broad range of organic matter and is not specific to humics. The comparison included for all samples: weight average molecular weight ( M w ), number average molecular weight ( M n ), polydispersity ( ρ ), absorbance at 280 nm normalized to moles C ( ε 280 ) (RFW and isolates); and for isolates only: elemental analysis, % carbon distribution by 13 C NMR, and aqueous FTIR spectra. As expected, RO isolation gave higher yield of NOM than XAD-8, but also higher ash content, especially Si and S. M w decreased in the order: RO>XAD-8>RFW>XAD-4. The M w differences of isolates compared with RFW may be due to selective isolation (fractionation), or possibly in the case of RO to condensation or coagulation during isolation. 13 C NMR results were roughly similar for the two methods, but the XAD-8 isolate was slightly higher in ‘aromatic’ C and the RO isolate was slightly higher in heteroaliphatic and carbonyl C. Infrared spectra indicated a higher carboxyl content for the XAD-8 isolates and a higher ester:carboxyl ratio for the RO isolates. The spectroscopic data thus are consistent with selective isolation of more hydrophobic compounds by XAD-8, and also with potential ester hydrolysis during that process, although further study is needed to determine whether ester hydrolysis does indeed occur. Researchers choosing between XAD and RO isolation methods for NOM need to consider first the purpose of the isolation; i.e., whether humic fractionation is desirable. Beyond that, they should consider the C yield and ash content, as well as the potential for alteration of NOM by ester hydrolysis (XAD) or condensation/coagulation (RO). Furthermore, the RO and XAD methods produce different fractions or isolates so that researchers should be careful when comparing the compositions and reactivities of NOM samples isolated by these two different techniques.

Water Research↗

The effect of research activities and winter precipitation on voiding behaviour of Agassiz’s desert tortoises ( Gopherus agassizii )

Context: There is little information available on how research activities might cause stress responses in wildlife, especially responses of threatened species such as the desert tortoise ( Gopherus agassizii ). Aims: The present study aims to detect behavioural effects of researcher handling and winter precipitation on a natural population of desert tortoises in the desert of Southwestern United States, over the period 1997 to 2014, through extensive assessments of capture events during multiple research studies, and capture&ndash;mark&ndash;recapture survivorship analysis. Methods: Juvenile and adult desert tortoises were repeatedly handled with consistent methodology across 18 years during 10 study seasons. Using a generalised linear mixed-effects model, we assessed the effects of both research manipulation and abiotic conditions on probability of voiding. Additionally, we used a Cormack&ndash;Jolly&ndash;Seber model to assess the effects of winter precipitation and voiding on long-term apparent survivorship. Key results: Of 1008 total capture events, voiding was recorded on 83 (8.2%) occasions in 42 different individuals. Our top models indicated that increases in handling time led to significantly higher probabilities of voiding for juveniles, females and males. Similarly, increases in precipitation resulted in significantly higher probabilities of voiding for juveniles and females, but not for males. Tortoise capture frequency was negatively correlated with voiding occurrence. Cormack&ndash;Jolly&ndash;Seber models demonstrated a weak effect of winter precipitation on survivorship, but a negligible effect for both voiding behaviour and sex. Conclusions: Handling-induced voiding by desert tortoises may occur during common research activities and years of above average winter precipitation. Increased likelihood of voiding in individuals with relatively low numbers of recaptures suggested that tortoises may have perceived researchers initially as predators, and therefore voided as a defensive strategy. Voiding does not appear to impact long-term survivorship in desert tortoises at this site. Implications: This study has demonstrated that common handling practices on desert tortoise may cause voiding behaviour. These results suggest that in order to minimise undesirable behavioural responses in studied desert tortoise populations, defined procedures or protocols must be followed by the investigators to reduce contact period to the extent feasible.

Wildlife Research↗

Leveraging invasive mussel contaminant survey data for stepwise prioritization of chemicals of potential concern in the Great Lakes basin

Historical and ongoing anthropogenic activities coupled with advancements in analytical techniques have led to the detection of large numbers of contaminants in the Laurentian Great Lakes. Consequently, identifying and prioritizing chemicals likely to cause ecological harm represents a challenge for natural resource managers. Previous prioritization efforts have focused on contaminants in sediment, water, and passive samplers, which may not be representative of compounds that bioaccumulate in aquatic organisms. Consequently, this study adopted a stepwise method to prioritize chemicals of potential concern detected in dreissenid mussels from samples collected across the Great Lakes from 2009–2018. The stepwise method considered environmental fate, detection frequency, and exceedance of toxicity quotients based on ecotoxicological effect concentrations. Overall, 153 compounds out of 267 analyzed were detected in dreissenid mussels, 47 of which had water quality effect concentrations, 56 had apical effect concentrations (Tier 1 ECOTOX or apical screening), 17 had nonapical effect concentrations (Tier 2 ECOTOX, Cytotoxic Burst, and ToxCast) and 33 had estimated effect concentrations (quantitative structure-activity relationship, estimated screening, and pharmacological potency). Of the compounds with water quality effect concentrations, nine were designated as high priority, including the herbicide atrazine and five polycyclic aromatic hydrocarbons that were previously identified as potentially hazardous within other matrices. Similar contaminants were identified as high priority in a related study of native unionid mussels in the Great Lakes. A total of 27 compounds were low priority, suggesting that these contaminants do not warrant further action based on this dataset. Overall, these findings will facilitate the development of management strategies to mitigate the effects of contaminants on aquatic organisms within the Great Lakes.

Great Lakes basin↗

Analysis of the trap gene provides evidence for the role of elevation and vector abundance in the genetic diversity of Plasmodium relictum in Hawaii

Background: The avian disease system in Hawaii offers an ideal opportunity to investigate host-pathogen interactions in a natural setting. Previous studies have recognized only a single mitochondrial lineage of avian malaria (Plasmodium relictum) in the Hawaiian Islands, but cloning and sequencing of nuclear genes suggest a higher degree of genetic diversity. Methods: In order to evaluate genetic diversity of P. relictum at the population level and further understand host-parasite interactions, a modified single-base extension (SBE) method was used to explore spatial and temporal distribution patterns of single nucleotide polymorphisms (SNPs) in the thrombospondin-related anonymous protein (trap) gene of P. relictum infections from 121 hatch-year amakihi (Hemignathus virens) on the east side of Hawaii Island. Results: Rare alleles and mixed infections were documented at three of eight SNP loci; this is the first documentation of genetically diverse infections of P. relictum at the population level in Hawaii. Logistic regression revealed that the likelihood of infection with a rare allele increased at low-elevation, but decreased as mosquito capture rates increased. The inverse relationship between vector capture rates and probability of infection with a rare allele is unexpected given current theories of epidemiology developed in human malarias. Conclusions: The results of this study suggest that pathogen diversity in Hawaii may be driven by a complex interaction of factors including transmission rates, host immune pressures, and parasite-parasite competition.

Hawai'i↗

Hierarchical distance-sampling models to estimate population size and habitat-specific abundance of an island endemic

Population size and habitat-specific abundance estimates are essential for conservation management. A major impediment to obtaining such estimates is that few statistical models are able to simultaneously account for both spatial variation in abundance and heterogeneity in detection probability, and still be amenable to large-scale applications. The hierarchical distance-sampling model of J. A. Royle, D. K. Dawson, and S. Bates provides a practical solution. Here, we extend this model to estimate habitat-specific abundance and rangewide population size of a bird species of management concern, the Island Scrub-Jay ( Aphelocoma insularis ), which occurs solely on Santa Cruz Island, California, USA. We surveyed 307 randomly selected, 300 m diameter, point locations throughout the 250-km 2 island during October 2008 and April 2009. Population size was estimated to be 2267 (95% CI 1613-3007) and 1705 (1212-2369) during the fall and spring respectively, considerably lower than a previously published but statistically problematic estimate of 12 500. This large discrepancy emphasizes the importance of proper survey design and analysis for obtaining reliable information for management decisions. Jays were most abundant in low-elevation chaparral habitat; the detection function depended primarily on the percent cover of chaparral and forest within count circles. Vegetation change on the island has been dramatic in recent decades, due to release from herbivory following the eradication of feral sheep ( Ovis aries ) from the majority of the island in the mid-1980s. We applied best-fit fall and spring models of habitat-specific jay abundance to a vegetation map from 1985, and estimated the population size of A. insularis was 1400-1500 at that time. The 20-30% increase in the jay population suggests that the species has benefited from the recovery of native vegetation since sheep removal. Nevertheless, this jay's tiny range and small population size make it vulnerable to natural disasters and to habitat alteration related to climate change. Our results demonstrate that hierarchical distance-sampling models hold promise for estimating population size and spatial density variation at large scales. Our statistical methods have been incorporated into the R package unmarked to facilitate their use by animal ecologists, and we provide annotated code in the Supplement.

California↗

Management and research applications of long-range surveillance radar data for birds, bats, and flying insects

There is renewed interest in using long-range surveillance radar as a biological research tool due to substantial improvements in the network of radars within the United States. Technical improvements, the digital nature of the radar data, and the availability of computing power and geographic information systems, enable a broad range of biological applications. This publication provides a summary of long-range surveillance radar technology and applications of these data to questions about movement patterns of birds and other flying wildlife. The intended audience is potential radar-data end users, including natural-resource management and regulatory agencies, conservation organizations, and industry. This summary includes a definition of long-range surveillance radar, descriptions of its strengths and weaknesses, information on applications of the data, cost, methods of calibration, and what end users need to do, and some key references and resources.

Texas↗

Simulation of daily streamflow for nine river basins in eastern Iowa using the Precipitation-Runoff Modeling System

The U.S. Geological Survey, in cooperation with the Iowa Department of Natural Resources, constructed Precipitation-Runoff Modeling System models to estimate daily streamflow for nine river basins in eastern Iowa that drain into the Mississippi River. The models are part of a suite of methods for estimating daily streamflow at ungaged sites. The Precipitation-Runoff Modeling System is a deterministic, distributed- parameter, physical-process-based modeling system developed to evaluate the response of streamflow and general drainage basin hydrology to various combinations of climate and land use. Calibration and validation periods used in each basin mostly were October 1, 2002, through September 30, 2012, but differed depending on the period of record available for daily mean streamflow measurements at U.S. Geological Survey streamflow-gaging stations. A geographic information system tool was used to delineate each basin and estimate values for model parameters based on basin physical and geographical features. A U.S. Geological Survey auto-calibration tool that uses a shuffled complex evolution algorithm was used for initial calibration, and then manual modifications were made to parameter values to complete the calibration of each basin model. The main objective of the calibration was to match daily discharge values of simulated streamflow to measured daily discharge values. The accuracy of Precipitation-Runoff Modeling System model streamflow estimates of nine river basins in eastern Iowa as compared to measured values at U.S. Geological Survey streamflow-gaging stations varied. The Precipitation-Runoff Modeling System models of nine river basins in eastern Iowa were satisfactory at estimating daily streamflow at 57 of the 79 calibration sites and 13 of the 14 validation sites based on statistical results. Unsatisfactory performance can be contributed to several factors: (1) low flow, no flow, and flashy flow conditions in headwater subbasins having a small drainage area; (2) poor representation of the groundwater and storage components of flow within a basin; (3) lack of accounting for basin withdrawals and water use; and (4) the availability and accuracy of meteorological input data. The Precipitation- Runoff Modeling System models of nine river basins in eastern Iowa will provide water-resource managers with a consistent and documented method for estimating streamflow at ungaged sites and aid in environmental studies, hydraulic design, water management, and water-quality projects.

Iowa↗

Stormwater quality of infrastructure elements in Rapid City, South Dakota, 2016–18

As runoff flows over the land or impervious surfaces (paved streets, parking lots, and building roofs), it accumulates debris, chemicals, sediment, and other contaminants that can adversely affect water quality if the runoff discharge remains untreated. Pathogens, commonly measured using fecal indicator bacteria such as Escherichia coli , enterococci, or fecal coliform, are the most-frequent cause of water-quality impairment in rivers and streams in the United States. Rapid Creek originates in the western Black Hills area and flows east through Rapid City, South Dakota, to its mouth at the Cheyenne River. The water quality of Rapid Creek is important because the reach that flows through Rapid City is a valuable spawning area for a self-sustaining trout fishery, is actively used for recreation, and is a seasonal municipal water supply for the City of Rapid City. These uses (fishery, recreation, and water supply) are considered beneficial uses by the South Dakota Department of Environment and Natural Resources. Numerical criteria have been established for total suspended solids and Escherichia coli concentrations, among other water-quality constituents, for these beneficial uses. The objectives of this study were to improve the method by which fecal indicator bacteria and total suspended solids are quantified in the urban drainages within Rapid City and to provide information that helps identify origins of fecal indicator bacteria and total suspended solids. This information can be used in hydrologic models to estimate fecal indicator bacteria and total suspended solid loading from certain infrastructure elements in urban environments. Stormwater samples analyzed for Escherichia coli , total suspended solids, specific conductance, and pH were collected in three drainage basin flowpaths within Rapid City: Jackson, Wildwood, and the Eco Prayer Park. Data-collection activities for this study focused on upgradient urban flowpath elements during rainfall events. This approach builds upon previous stormwater assessments that characterized the water quality in urban basin outlets near the downstream end of the stormwater flowpaths. Within each flowpath group, 4–6 sites were selected to represent the various infrastructure elements of the runoff process. These elements included roof downspouts, parking lots, street curbs and gutters, open channels, underground storm sewers, and stormwater ponds or best-management practice facilities. In general, the concentrations of Escherichia coli and total suspended solids increased in the downstream direction for all flowpath sites. The wash-off process after the first flush is evident for total suspended solids and specific conductance; however, Escherichia coli concentrations did not necessarily follow the same pattern. Escherichia coli concentrations in the latter part of the runoff period were similar to or greater than the initial concentrations of the first set of samples. Stormwater-quality data were summarized by infrastructure type (roof downspout, parking lot, street curb, and channel/storm sewer) to provide information about approximate water-quality concentrations originating at the upper end of urban flowpaths. Escherichia coli and total suspended solid concentrations were lowest in samples collected from locations most isolated from human influence (roof downspouts); the median concentrations at these sites were 4 most probable number per 100 milliliters and 15 milligrams per liter, respectively. The delivery potential of fecal indicator bacteria and sediment from parking lots and street curbs was similar; median concentrations of Escherichia coli and total suspended solids were around 150–220 most probable number per 100 milliliters and 56–86 milligrams per liter, respectively. The downstream receiving channels and storm sewers where stormwater was aggregated typically contained the highest Escherichia coli concentrations (median was 1,800 most probable number per 100 milliliters), but the total suspended solid concentrations were similar to upstream elements in the flowpath (median was 69 milligrams per liter). The data collected from this study demonstrate that stormwater is contaminated with fecal indicator bacteria upon initial contact with impervious surfaces and highlight the importance of controlling the volume of stormwater discharges into receiving waterbodies via storage structures and pervious elements. Diluting stormwater with high concentrations of Escherichia coli with the receiving water’s (Rapid Creek) lower concentration of Escherichia coli is likely the primary mechanism for meeting the beneficial-use criterion threshold of 235 most probable number per 100 milliliters. Although total suspended solid concentrations in the upper parts of the basin (parking lots and street curbs) also begin at concentrations (56 to 86 milligrams per liter) above the beneficial-use criterion for Rapid Creek (53 milligrams per liter), current stormwater-control practices (storage ponds, swales, and wetlands) may be able to reduce suspended-sediment concentrations to meet this threshold.

South Dakota↗

Transmissivity estimated from brief aquifer tests of domestic wells and compared with bedrock lithofacies and position on hillsides in the Appalachian Plateau of New York

Procedures for undertaking and analyzing recovery from aquifer tests of 13 to 132 seconds (described in reports cited herein) were applied to 51 domestic drilled wells that penetrated bedrock outside major valleys in the part of the Appalachian Plateau of New York drained by the Susquehanna River. Transmissivities calculated from these tests ranged over three orders of magnitude in both the Catskill-Cattaraugus lithofacies (shales, mudstones, siltstones, medium to coarse sandstones, pebbly sandstones) and the Chemung-Hamilton lithofacies (shales, mudstones, siltstones, fine to medium sandstones). Median transmissivity values were 0.000425 foot squared per second (36.7 feet squared per day) in the Catskill-Cattaraugus lithofacies and 0.00055 foot squared per second (47.5 feet squared per day) in the Chemung-Hamilton lithofacies. The distributions of transmissivity values within the two lithofacies were likewise similar. The range and median values of transmissivity were also nearly the same on lower and midlevel hillsides and were only slightly greater on a few upper hillsides. Transmissivities estimated from such easily arranged and analyzed tests may be appropriate for estimating groundwater flux under the small gradients that prevail under natural conditions, but not under larger drawdowns and steeper gradients near clusters of domestic wells. Four of the 51 wells tested were also pumped for 10 to 32 minutes; analysis by the Theis recovery method yielded transmissivities consistent with the brief tests for 2 wells, but 7 to 9 times smaller for 2 wells. Transmissivity values estimated by the PICKINGmodel were not significantly different from values estimated by an automated application of the Picking method (PPC-Recovery) at a probability of 95 percent. Transmissivities calculated by either method from data for time intervals of 120 seconds or less may be of limited practical value because they apply only to a small volume of bedrock close to the pumped well.

New York↗

Pathogenecity of Ichthyophonus hoferi for laboratory-reared Pacific herring ( Clupea pallasi ) and its early appearance in wild Puget Sound herring

Laboratory-reared pathogen-free Pacific herring were exposed to pure cultures of Ichthyophonus hoferi, and reproduced the disease seen in naturally infected fish--thus fulfilling Koch's Postulates. Pathogen-free herring used in this study were reared from artificially spawned eggs incubated in filtered, UV-sterilized seawater, eliminating the variables associated with multiple infections, which are common in wild herring. Wild free-ranging herring were captured monthly from June through October by dip net from 'herring balls' located in the northern Puget Sound. I. hoferi infections were identified in these fish soon after metamorphoses, about 4 mo post-hatch. The prevalence increased from 5 to 6% in 0-yr fish to 24% in 1-yr-old fish to 50 to 70% in fish over 2 yr old, with no associated increase in mortality. The route of natural transmission to wild herring was not determined, but carnivorous fish became infected and died when they were experimentally fed tissues infected with the organism. In vitro culture of tissues was the most sensitive method for identifying both clinical and subclinical infections.

Washington↗

Surface flow velocities from space: Particle image velocimetry of satellite video of a large, sediment-laden river

Conventional, field-based streamflow monitoring in remote, inaccessible locations such as Alaska poses logistical challenges. Safety concerns, financial considerations, and a desire to expand water-observing networks make remote sensing an appealing alternative means of collecting hydrologic data. In an ongoing effort to develop non-contact methods for measuring river discharge, we evaluated the potential to estimate surface flow velocities from satellite video of a large, sediment-laden river in Alaska via particle image velocimetry (PIV). In this setting, naturally occurring sediment boil vortices produced distinct water surface features that could be tracked from frame to frame as they were advected by the flow, obviating the need to introduce artificial tracer particles. In this study, we refined an end-to-end workflow that involved stabilization and geo-referencing, image preprocessing, PIV analysis with an ensemble correlation algorithm, and post-processing of PIV output to filter outliers and scale and geo-reference velocity vectors. Applying these procedures to image sequences extracted from satellite video allowed us to produce high resolution surface velocity fields; field measurements of depth-averaged flow velocity were used to assess accuracy. Our results confirmed the importance of preprocessing images to enhance contrast and indicated that lower frame rates (e.g., 0.25 Hz) lead to more reliable velocity estimates because longer capture intervals allow more time for water surface features to translate several pixels between frames, given the relatively coarse spatial resolution of the satellite data. Although agreement between PIV-derived velocity estimates and field measurements was weak ( R 2 = 0.39) on a point-by-point basis, correspondence improved when the PIV output was aggregated to the cross-sectional scale. For example, the correspondence between cross-sectional maximum velocities inferred via remote sensing and measured in the field was much stronger ( R 2 = 0.76), suggesting that satellite video could play a role in measuring river discharge. Examining correlation matrices produced as an intermediate output of the PIV algorithm yielded insight on the interactions between image frame rate and sensor spatial resolution, which must be considered in tandem. Although further research and technological development are needed, measuring surface flow velocities from satellite video could become a viable tool for streamflow monitoring in certain fluvial environments.

Alaska↗