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At least 991 records · Page 55Linked to original sources

Persistence of historical population structure in an endangered species despite near-complete biome conversion in California's San Joaquin Desert

Genomic responses to habitat conversion can be rapid, providing wildlife managers with time-limited opportunities to enact recovery efforts that use population connectivity information that reflects predisturbance landscapes. Despite near-complete biome conversion, such opportunities may still exist for the endemic fauna and flora of California's San Joaquin Desert, but comprehensive genetic data sets are lacking for nearly all species in the region. To fill this knowledge gap, we studied the rangewide population structure of the endangered blunt-nosed leopard lizard Gambelia sila , a San Joaquin Desert endemic, using restriction site-associated DNA (RAD), microsatellite and mtDNA data to test whether admixture patterns and estimates of effective migration surfaces (EEMS) can identify land areas with high population connectivity prior to the conversion of native xeric habitats. Clustering and phylogenetic analyses indicate a recent shared history between numerous isolated populations and EEMS reveals latent signals of corridors and barriers to gene flow over areas now replaced by agriculture and urbanization. Conflicting histories between the mtDNA and nuclear genomes are consistent with hybridization with the sister species G. wislizenii , raising important questions about where legal protection should end at the southern range limit of G. sila . Comparative analysis of different data sets also adds to a growing list of advantages in using RAD loci for genetic studies of rare species. We demonstrate how the results of this work can serve as an evolutionary guidance tool for managing endemic, arid-adapted taxa in one of the world's most compromised landscapes.

California↗

Winter carry-over effects on spring body condition driven by agricultural subsidies to Lesser Snow Geese (Anser caersulscens caerulescens)

Anthropogenic changes to landscapes associated with intensive agriculture often have deleterious effects on avian abundance. However, some species like the Lesser Snow Goose ( Anser caerulescens caerulescens ), can benefit from increases in agricultural crops on both wintering and migratory stopover sites. We investigated the influence of winter habitat use on spring body condition in Lesser Snow Goose, a species that has increased in population following expansion into agriculturally based winter habitats. We used stable isotope measurements of four elements (δ 2 H, δ 13 C, δ 15 N, and δ 34 S) to determine likely prior winter habitat use of snow geese collected during spring migration across Arkansas, Missouri, and Nebraska in 2016. We evaluated differences in body size, lipid, and protein reserves from individuals with isotope values that suggested winter habitat use in traditional coastal marsh and non-coastal /agriculture habitat. Inferred winter habitat influenced total body lipid levels in snow geese collected during spring migration. Adult and juvenile individuals inferred to have overwintered in coastal marsh (n = 60) had, on average, 33.4 g (95% Confidence Interval: 0.4 g, 66.4 g) less lipid than counterparts wintering in non-coastal / agricultural habitat (n = 77). Waterfowl foods found in marshes typically have low true metabolizable energy values as a consequence of their high fiber content, which likely increases daily consumption rates. Increased energy expenditure related to greater time spent foraging, paired with lower energetic rewards, may result in lower lipid reserves among geese using coastal marsh habitats compared to birds using agricultural landscapes. Consequently, carry-over effects based on winter habitat use could explain variation in lipid reserves among individuals during spring migration and may ultimately explain differential fitness rates or susceptibility to harvest. Our results have implications for the conservation and management of this species as historic wetland landscapes become more intensively converted and used for agricultural purposes.

Arkansas, Missouri, Nebraska↗

A global analysis of traits predicting species sensitivity to habitat fragmentation

Aim Elucidating patterns in species responses to habitat fragmentation is an important focus of ecology and conservation, but studies are often geographically restricted, taxonomically narrow or use indirect measures of species vulnerability. We investigated predictors of species presence after fragmentation using data from studies around the world that included all four terrestrial vertebrate classes, thus allowing direct inter-taxonomic comparison. Location World-wide. Methods We used generalized linear mixed-effect models in an information theoretic framework to assess the factors that explained species presence in remnant habitat patches (3342 patches; 1559 species, mostly birds; and 65,695 records of patch-specific presence–absence). We developed a novel metric of fragmentation sensitivity, defined as the maximum rate of change in probability of presence with changing patch size (‘Peak Change’), to distinguish between general rarity on the landscape and sensitivity to fragmentation per se. Results Size of remnant habitat patches was the most important driver of species presence. Across all classes, habitat specialists, carnivores and larger species had a lower probability of presence, and those effects were substantially modified by interactions. Sensitivity to fragmentation (measured by Peak Change) was influenced primarily by habitat type and specialization, but also by fecundity, life span and body mass. Reptiles were more sensitive than other classes. Grassland species had a lower probability of presence, though sample size was relatively small, but forest and shrubland species were more sensitive. Main conclusions Habitat relationships were more important than life-history characteristics in predicting the effects of fragmentation. Habitat specialization increased sensitivity to fragmentation and interacted with class and habitat type; forest specialists and habitat-specific reptiles were particularly sensitive to fragmentation. Our results suggest that when conservationists are faced with disturbances that could fragment habitat they should pay particular attention to specialists, particularly reptiles. Further, our results highlight that the probability of presence in fragmented landscapes and true sensitivity to fragmentation are predicted by different factors.

Global Ecology and Biogeography↗

Human influence on California fire regimes

Periodic wildfire maintains the integrity and species composition of many ecosystems, including the mediterranean-climate shrublands of California. However, human activities alter natural fire regimes, which can lead to cascading ecological effects. Increased human ignitions at the wildland-urban interface (WUI) have recently gained attention, but fire activity and risk are typically estimated using only biophysical variables. Our goal was to determine how humans influence fire in California and to examine whether this influence was linear, by relating contemporary (2000) and historic (1960-2000) fire data to both human and biophysical variables. Data for the human variables included fine-resolution maps of the WUI produced using housing density and land cover data. Interface WUI, where development abuts wildland vegetation, was differentiated from intermix WUI, where development intermingles with wildland vegetation. Additional explanatory variables included distance to WUI, population density, road density, vegetation type, and ecoregion. All data were summarized at the county level and analyzed using bivariate and multiple regression methods. We found highly significant relationships between humans and fire on the contemporary landscape, and our models explained fire frequency (R2 = 0.72) better than area burned (R2 = 0.50). Population density, intermix WUI, and distance to WUI explained the most variability in fire frequency, suggesting that the spatial pattern of development may be an important variable to consider when estimating fire risk. We found nonlinear effects such that fire frequency and area burned were highest at intermediate levels of human activity, but declined beyond certain thresholds. Human activities also explained change in fire frequency and area burned (1960-2000), but our models had greater explanatory power during the years 1960-1980, when there was more dramatic change in fire frequency. Understanding wildfire as a function of the spatial arrangement of ignitions and fuels on the landscape, in addition to nonlinear relationships, will be important to fire managers and conservation planners because fire risk may be related to specific levels of housing density that can be accounted for in land use planning. With more fires occurring in close proximity to human infrastructure, there may also be devastating ecological impacts if development continues to grow farther into wildland vegetation. ?? 2007 by the Ecological Society of America.

Ecological Applications↗

Hibernal habitat selection by Wood Frogs ( Lithobates sylvaticus ) in a northern New England montane landscape

Poikilothermic species, such as amphibians, endure harsh winter conditions via freeze-tolerance or freeze-avoidance strategies. Freeze-tolerance requires a suite of complex, physiological mechanisms (e.g., cryoprotectant synthesis); however, behavioral strategies (e.g., hibernal habitat selection) may be used to regulate hibernaculum temperatures and promote overwintering survival. We investigated the hibernal ecology of the freeze-tolerant Wood Frog ( Lithobates sylvaticus ) in north-central Maine. Our objectives were to characterize the species hibernaculum microclimate (temperature, relative humidity), evaluate hibernal habitat selection, and describe the spatial arrangement of breeding, post-breeding, and hibernal habitats. We monitored 15 frogs during two winters (2011/12: N = 10; 2012/13: N = 5), measured hibernal habitat features at micro (2 m) and macro (10 m) spatial scales, and recorded microclimate hourly in three strata (hibernaculum, leaf litter, ambient air). We compared these data to that of 57 random locations with logistic regression models, Akaike Information Criterion, and Kolmogorov–Smirnov tests. Hibernaculum microclimate was significantly different and less variable than leaf litter, ambient air, and random location microclimate. Model averaging indicated that canopy cover (−), leaf litter depth (+), and number of logs and stumps (+; microhabitat only) were important predictors of Wood Frog hibernal habitat. These habitat features likely act to insulate hibernating frogs from extreme and variable air temperatures. For example, decreased canopy cover facilitates increased snowpack depth and earlier snowpack accumulation and melt. Altered winter temperature and precipitation patterns attributable to climate change may reduce snowpack insulation, facilitate greater temperature variation in the underlying hibernacula, and potentially compromise Wood Frog winter survival.

Maine↗

Mountain sentinels in a changing world: Review and conservation implications of weather and climate effects on mountain goats (Oreamnos americanus)

Climate change is occurring at an accelerated rate in high-elevation alpine and mountain ecosystems. Cold-adapted, mountain species are at risk due to forecasted change and knowledge is needed to respond to current and future conservation challenges. Mountain goats ( Oreamnos americanus ) are an iconic species of North American mountain cultures and landscapes, and due to specialized adaptations for life in cold, mountainous environments they are particularly sensitive to changes in weather and climate. As sentinels of change in alpine ecosystems, the study of mountain goats offers insight into the ecological effects and conservation challenges associated with climate change in these sensitive and biodiverse environments. Here, we synthesize existing knowledge about how climate change is expected to influence environmental conditions experienced by mountain goats and associated mechanistic changes to behavior, nutritional ecology, demography, health, and interspecific interactions. In many instances, climate change effects are likely to be negative and additive to existing threats (such as human disturbance, hunting, disease, predation) though benefits are expected in some cases. Changes in climate and mountain environments will necessitate re-examination and modification of population monitoring, management, and conservation strategies. Specifically, spatiotemporal (and other) aspects of monitoring and management may need to be adjusted to accommodate emerging and novel conservation challenges. Yet, key data and knowledge gaps remain and should be addressed to advance conservation and decision-making capabilities. For mountain goats and similarly climate-sensitive alpine herbivores, effective conservation will ultimately benefit from collaborations among diverse networks guided by well-planned, strategic visions focused on common ground – namely the resiliency and persistence of culturally and ecologically significant mountain species and the alpine environment they inhabit.

Alaska, Alberta, British Columbia, Idaho, Montana,↗

Riverscape nesting dynamics of Neosho Smallmouth Bass: To cluster or not to cluster?

Aim Hierarchical stream habitat conditions influence patterns of fish abundance and population dynamics. The spawning period is important for stream fishes but coincides with unpredictable environmental conditions and stressors. Thus, identifying habitats that confer suitable spawning is crucial to managing vulnerable fish populations, including narrow-range endemics. Here, we evaluate reach- and catchment-scale habitat features related to Neosho Smallmouth Bass ( Micropterus dolomieu velox ) nest presence, abundance and aggregations (clusters) and quantify nest microhabitat. Location Ozark Highlands ecoregion, USA. Methods We conducted snorkel and habitat surveys from 2016 to 2018 to quantify nest abundance, describe nest cluster characteristics and quantify nest microhabitat. We used field-collected and geospatial variables and developed generalized mixed models to evaluate the influence of multi-scale habitat features on nest cluster presence and nest abundance. Results Nest clusters, scarcely known for other Smallmouth Bass populations, contained 25% of all documented nests. Presence of nests was more likely in warmer stream reaches with wide, shallow channels and more pool habitat. Nest cluster presence was more likely with greater nest densities and earlier in the spawning season. The abundance of Smallmouth Bass nests was related to several reach-scale habitat conditions, with greater nest counts in warmer reaches and reaches with deeper pool habitat. Regardless of cluster behaviour, nesting Smallmouth Bass used similar microhabitats, including a range of depths (0.26–1.85 m), low velocities (<0.1 m/s) and typically gravel substrates. Main conclusions Our results indicate plasticity in nesting ecology within Neosho Smallmouth Bass populations and highlight the need to consider multiple aspects of stream habitat when developing conservation and management plans. The importance of reach-scale habitat features suggests it may be important to limit landscape and channel alterations. Nest clustering behaviour suggests these populations may be vulnerable to human influence during the nesting season, but also provides management opportunities for protection during critical time periods.

Arkansas, Kansas, Missouri, Oklahoma↗

Using scenarios to evaluate vulnerability of grassland communities to climate change in the Southern Great Plains of the United States

Scenario planning is a useful tool for identifying key vulnerabilities of ecological systems to changing climates, informed by the potential outcomes for a set of divergent, plausible, and relevant climate scenarios. We evaluated potential vulnerabilities of grassland communities to changing climate in the Southern Great Plains (SGP) and the Landscape Conservation Design pilot area (LCD) for the U.S. Fish and Wildlife Service, Science Applications Program, Great Plains Landscape Conservation Cooperative. Four climate scenarios (warm-dry, warm-wet, hot-dry, and hot-wet) from atmospheric-ocean general circulation models were selected to represent a suite of plausible future climatic conditions. For each scenario, and for contemporary climatic conditions, we predicted the spatial patterns of relative productivity for indicator grass species using statistical models of relative above-ground net primary productivity (hereafter, productivity) based on temperature, precipitation, and soil texture (percent sand, silt, or clay). Two indicator grass species were selected to represent each of four focal grassland communities: semi-desert grasslands, shortgrass prairie, mixed-grass prairie, and tallgrass prairie. Changes in spatial patterning of bioclimatic conditions conducive for each indicator species as predicted for each climate scenario relative to current land use were used to evaluate potential vulnerability and conservation opportunities for grassland communities. Specifically, the following questions were addressed for each focal grassland community: (1) Where is the productivity of each species predicted to increase, decrease, or remain stable relative to estimated contemporary productivity for the SGP and LCD pilot area, (2) where is the productivity of the two indicator species for each community predicted to increase, decrease, or remain stable, (3) which grassland communities are most vulnerable to changes in composition and vertical structure, (4) how do current land-use patterns contribute to potential vulnerabilities of grassland communities for the climate scenarios evaluated, and (5) how can managers use the vulnerabilities identified to evaluate conservation opportunities in the SGP and LCD? Current land-use patterns, in combination with the potential effects of a changing climate, pose greater risks to mixed-grass and tallgrass prairies of the SGP compared to semi-desert grasslands and shortgrass prairie. For most climate scenarios evaluated, bioclimatic conditions conducive to the taller species were predicted to contract within some or all the current distribution of mixed-grass and tallgrass prairies within the SGP. An increase in precipitation, however, could potentially ameliorate the negative effects of increasing temperatures as evidenced by higher productivity for the hot-wet scenario compared to the other scenarios for the most vulnerable species. Compounding their greater vulnerability to increasing temperatures coupled with decreasing precipitation, the mixed-grass and tallgrass prairies have been greatly fragmented and converted, primarily by agriculture. In contrast, the climate scenarios evaluated are generally conducive to stable or increasing productivity of indicator species for semi-desert grasslands and shortgrass prairie. In addition, conversion and fragmentation of semi-desert grasslands and shortgrass prairie were relatively low. These results suggest that the synergistic effects of land use and changing climatic conditions could have the greatest effects on the composition and structure of mixed-grass and tallgrass prairies in the SGP. ScienceBase data release files that support this report are available at https://doi.org/10.5066/P9DGJHEP (Manier and others, 2019).

Colorado, Kansas, Nebraska, New Mexico, Oklahoma, ↗

Selecting sagebrush seed sources for restoration in a variable climate: ecophysiological variation among genotypes

Big sagebrush ( Artemisia tridentata ) communities dominate a large fraction of the United States and provide critical habitat for a number of wildlife species of concern. Loss of big sagebrush due to fire followed by poor restoration success continues to reduce ecological potential of this ecosystem type, particularly in the Great Basin. Choice of appropriate seed sources for restoration efforts is currently unguided due to knowledge gaps on genetic variation and local adaptation as they relate to a changing landscape. We are assessing ecophysiological responses of big sagebrush to climate variation, comparing plants that germinated from ~20 geographically distinct populations of each of the three subspecies of big sagebrush. Seedlings were previously planted into common gardens by US Forest Service collaborators Drs. B. Richardson and N. Shaw, (USFS Rocky Mountain Research Station, Provo, Utah and Boise, Idaho) as part of the Great Basin Native Plant Selection and Increase Project. Seed sources spanned all states in the conterminous Western United States. Germination, establishment, growth and ecophysiological responses are being linked to genomics and foliar palatability. New information is being produced to aid choice of appropriate seed sources by Bureau of Land Management and USFS field offices when they are planning seed acquisitions for emergency post-fire rehabilitation projects while considering climate variability and wildlife needs.

Report↗

Germination information for common Arizona restoration species

Seed-based ecological restoration is an approach used to revegetate damaged and disturbed habitats by spreading seed with the expectation that germination will occur and plants will become established and flourish. Although restoration can enhance the health and productivity of landscapes by reinvigorating ecosystem services both directly and indirectly, successful restoration is difficult to achieve – particularly in arid systems (Copeland et al. 2018). Germination is a well known bottleneck to plant growth that prohibits successful restoration (James at al. 2011).

Cooperative Extension Publication↗

Climate- and disturbance-driven changes in subsistence berries in coastal Alaska: Indigenous knowledge to inform ecological inference

Berry-producing plants are a key subsistence resource in Indigenous Alaskan communities. High-latitude coastal regions are particularly impacted by global climate change due to their location at the land-sea ecotone subjecting them to terrestrial stressors as well as shifts in ocean dynamics. While vegetation changes have been documented for the subarctic coastal region of Alaska, we know little about permafrost underlain portions of the landscape that support berry communities. To fill this gap surveys were administered in four communities within the Yukon-Kuskokwim Delta, to identify observations of changes in berry resources and integrate Indigenous Knowledge of drivers of changes with climate projections. We found that while most participants agreed on several observed changes and drivers, many important differences were identified at the community level. Regional consensus can be used in combination with climate projections to forecast potential future impacts to berry resources while distinctions at the community level can help us to untangle local scale disturbances.

Alaska↗

Changes in habitat availability for outmigrating juvenile salmon (Oncorhychus spp.) following estuary restoration

The restoration of the Nisqually River Delta (Washington, U.S.A.) represents one of the largest efforts toward reestablishing the ecosystem function and resilience of modified habitat in the Puget Sound, particularly for anadromous salmonid species. The opportunity for outmigrating salmon to access and benefit from the expansion of available tidal habitat can be quantified by several physical attributes, which are related to the ecological and physiological responses of juvenile salmon. We monitored a variety of physical parameters to measure changes in opportunity potential from historic, pre-restoration, and post-restoration habitat conditions at several sites across the delta. These parameters included channel morphology, water quality, tidal elevation, and landscape connectivity. We conducted fish catch surveys across the delta to determine if salmon was utilizing restored estuary habitat. Overall major channel area increased 42% and major channel length increased 131% from pre- to post-restoration conditions. Furthermore, the results of our tidal inundation model indicated that major channels were accessible up to 75% of the time, as opposed to 30% pre-restoration. Outmigrating salmon utilized this newly accessible habitat as quickly as 1 year post-restoration. The presence of salmon in restored tidal channels confirmed rapid post-restoration increases in opportunity potential on the delta despite habitat quality differences between restored and reference sites.

Washington↗

An exploratory investigation of the landscape-lake interface: Land cover controls over consumer N and C isotopic composition in Lake Michigan rivermouths

Rivermouth ecosystems are areas where tributary waters mix with lentic near-shore waters and provide habitat for many Laurentian Great Lakes fish and wildlife species. Rivermouths are the interface between terrestrial activities that influence rivers and the ecologically important nearshore. Stable isotopes of nitrogen (N) and carbon (C) in consumers were measured from a range of rivermouths systems to address two questions: 1) What is the effect of rivermouth ecosystems and land cover on the isotopic composition of N available to rivermouth consumers? 2) Are rivermouth consumers composed of lake-like or river-like C? For question 1, data suggest that strong relationships between watershed agriculture and consumer N are weakened or eliminated at the rivermouth, in favor of stronger relationships between consumer N and depositional areas that may favor denitrification. For question 2, despite apparently large riverine inputs, consumers only occasionally appear river-like. More often consumers seem to incorporate large amounts of C from either the nearshore or primary production within the rivermouth itself. Rivermouths appear to be active C and N processing environments, thus necessitating their explicit incorporation into models estimating nearshore loading and possibly contributing to their importance to Great Lakes biota.

Lake Michigan↗

Mountain glaciers influence biogeochemical and ecological characteristics of high-elevation lakes across the northern Rocky Mountains, USA

Mountain glaciers are retreating rapidly due to climate change, leading to the formation of downstream lakes. However, little is known about the physical and biogeochemical conditions in these lakes across a range of glacial influence. We surveyed alpine lakes fed by both glacial and snowpack meltwaters and those fed by snowpack alone to compare nutrient concentrations, stoichiometry, water clarity, chlorophyll, and zooplankton communities. Total phosphorus (TP) and soluble reactive phosphorus were two times higher in glacial lakes than in non-glacial lakes, while nitrate concentrations were three times higher. However, organic carbon concentrations in glacial lakes were two times lower than in non-glacial lakes. The carbon-to-phosphorus ratio and the nitrogen-to-phosphorus ratio of lake seston increased with water clarity in glacial lakes, suggesting that turbidity from glacial flour increases light limitation and increases stoichiometric food quality for zooplankton in newly formed lakes. However, chlorophyll a concentrations did not differ between lake types. Through structural equation modeling, we found that glaciers exhibit a bidirectional association with nitrate and TP concentrations, perhaps mediated through landscape vegetation and lake clarity. Zooplankton communities in high-turbidity glacial lakes were largely composed of cyclopoid copepods and rotifers (i.e., non-filter feeders), while non-glacial lakes tended to be dominated by calanoid copepods and cladocerans (i.e., filter feeders). Our results show that glacier-influenced lakes have biogeochemical and ecological characteristics distinct from snow-fed mountain lakes. Sustained studies are needed to assess the dynamics of these unique features as the influence of the alpine cryosphere fades under ongoing climate change.

Montana↗

Evaluating slash piles as habitat for a threatened salamander

Background Amplified wildfire activity in forests of the western United States threatens biodiversity. Fuel treatments can reduce fire severity, modify fire behavior, and restore forest structure and composition, yet impacts of some treatments, including slash piling and burning, on wildlife have received little attention. Piling of residual woody material may create habitable microenvironments for species that require cool, moist microclimates for all biological and ecological functions. One such species, the Sacramento Mountain salamander ( Aneides hardii Taylor), a relictual, endemic salamander narrowly distributed in the mountains of south-central New Mexico, USA, has been found below constructed slash piles within its range, but the characteristics of occupied slash piles and the extent of their occupancy has not yet been quantified. We surveyed for Sacramento Mountain salamanders in slash piles and under logs (cover objects) adjacent to piles and within a surrounding survey plot, and related salamander occupancy to slash pile and cover object characteristics, soil moisture and temperature, and environmental setting. Results We found Sacramento Mountain salamanders in 50% of surveyed slash piles. About 90% of salamanders were found in piles that contained black plastic sheeting, which held accumulations of moist leaf litter and other forest debris. We found no differences in pile characteristics, soil variables, or environmental setting between piles occupied by salamanders and piles in which no salamanders were detected. Salamander density was highest in slash piles, ~ 10% lower under cover objects in the survey area, and ~ 23% lower under cover objects adjacent to slash piles. Conclusions Slash piles serve as habitat for Sacramento Mountain salamanders. Our results suggest that within our study area or similar environments within the species range, any comparable slash pile has the potential to be occupied by salamanders. Species and habitat conservation measures indicated by this study and the timing of historical detections into mid-October include constructing smaller, pyramidal piles that minimize log-on-log or log-ground contact, avoiding the inclusion of black plastic in piles, limiting residence time of piles on the landscape, and initiating pile burning in late October or early November, when most salamanders are likely to have retreated below the ground surface.

New Mexico↗

Assessing the ecological functionality and integrity of natural ponds, excavated ponds and stormwater basins for conserving amphibian diversity

Wetlands provide ecological functionality by maintaining and promoting regional biodiversity supporting quality habitat for aquatic organisms. Globally, habitat loss, fragmentation and degradation due to increases in agricultural activities and urban development have reduced or altered geographically isolated wetlands, thus reducing biodiversity. The objective of this study was to assess the relative ecological function and integrity of natural ponds, excavated ponds and stormwater basins in the New Jersey Pinelands, located in the northeastern United States by comparing hydrologic conditions, water quality, pesticide concentrations (water, sediment and tissue) and wetland assemblages including amphibians. Twenty-four wetlands were selected based on surrounding land-use and sampled for a variety of abiotic and biotic variables. Abiotic and biotic wetland variables were similar between natural and excavated ponds, with notable differences between the ponds and stormwater basins. Natural and excavated ponds displayed characteristic Pinelands water quality (low pH, high organic carbon, and low pesticide concentrations), exhibited high ecological integrity and supported native ampbibians. Stormwater basins and degraded ponds surrounded by altered land-use exhibited degraded water quality (high pH, high pesticide concentrations) and were dominated by non-native and introduced plants and amphibians. Results from this study can broadly inform resource conservation strategies for amphibians and other communities with a diverse range of habitat requirements, particularly in areas where conservation and development are competing priorities. To conserve biodiversity in changing landscapes, wetlands with similar functionality and land-use characteristics need to be identified and managed to preserve water quality for species of conservation concern.

New Jersey↗

Estimation of late twentieth century land-cover change in California

We present the first comprehensive multi-temporal analysis of land-cover change for California across its major ecological regions and primary land-cover types. Recently completed satellite-based estimates of land-cover and land-use change information for large portions of the United States allow for consistent measurement and comparison across heterogeneous landscapes. Landsat data were employed within a pure-panel stratified one-stage cluster sample to estimate and characterize land-cover change for 1973–2000. Results indicate anthropogenic and natural disturbances, such as forest cutting and fire, were the dominant changes, followed by large fluctuations between agriculture and rangelands. Contrary to common perception, agriculture remained relatively stable over the 27-year period with an estimated loss of 1.0% of agricultural land. The largest net declines occurred in the grasslands/shrubs class at 5,131 km 2 and forest class at 4,722 km 2 . Developed lands increased by 37.6%, composing an estimated 4.2% of the state’s land cover by 2000.

California↗

A comparison of honey bee-collected pollen from working agricultural lands using light microscopy and ITS metabarcoding

Taxonomic identification of pollen has historically been accomplished via light microscopy but requires specialized knowledge and reference collections, particularly when identification to lower taxonomic levels is necessary. Recently, next-generation sequencing technology has been used as a cost-effective alternative for identifying bee-collected pollen; however, this novel approach has not been tested on a spatially or temporally robust number of pollen samples. Here, we compare pollen identification results derived from light microscopy and DNA sequencing techniques with samples collected from honey bee colonies embedded within a gradient of intensive agricultural landscapes in the Northern Great Plains throughout the 2010–2011 growing seasons. We demonstrate that at all taxonomic levels, DNA sequencing was able to discern a greater number of taxa, and was particularly useful for the identification of infrequently detected species. Importantly, substantial phenological overlap did occur for commonly detected taxa using either technique, suggesting that DNA sequencing is an appropriate, and enhancing, substitutive technique for accurately capturing the breadth of bee-collected species of pollen present across agricultural landscapes. We also show that honey bees located in high and low intensity agricultural settings forage on dissimilar plants, though with overlap of the most abundantly collected pollen taxa. We highlight practical applications of utilizing sequencing technology, including addressing ecological issues surrounding land use, climate change, importance of taxa relative to abundance, and evaluating the impact of conservation program habitat enhancement efforts.

Environmental Entomology↗