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Data on ground-water quality, Carson Valley and Topaz Lake areas, Douglas County, Nevada, for year ending September 1986

Douglas County, Nevada encompasses 708 sq mi and has been one of the fastest growing counties in the Nation, according to the 1980 census. This rapid population growth has led to concern about the present and future impacts of development upon the groundwater. A network of 33 wells was recently established (1985) in the Carson Valley and Topaz Lake areas of Douglas County to characterize the current groundwater quality and its seasonal variability and to monitor temporal responses to changing land-use activities. This report presents data collected from that network during November 1985 through September 1986. The primary drinking water standard was exceeded for fluoride at two domestic wells, for nitrate-nitrogen at one domestic well, and for arsenic in one non-domestic shallow monitoring well. Secondary drinking water standards were exceeded as follows: pH at one public-supply well and one domestic well; sulfate at one shallow monitoring well; and manganese at three shallow monitoring wells and one public-supply well. Concentrations of unidentified organic compounds were estimated by gas chromatograph and flame ionization detector for groundwater samples collected from 30 network wells. Seven wells (2 domestic and 5 non-domestic) had concentrations greater than 10 micrograms/L relative to the internal standard compound perdeuteronaphthalene. (Thacker-USGS)

Open-File Report↗

A method for quantifying bioavailable organic carbon in aquifer sediments

The fact that naturally occurring microorganisms can biodegrade PCE and TCE allows the use of monitored natural attenuation (MNA) as a remediation strategy at chlorinated solvent-contaminated sites. Research at numerous chlorinated solvent sites indicates an active dechlorinating microbial population coupled with an ample supply of organic carbon are conditions needed to sustain reductive dechlorination. A series of extraction experiments was used to compare the ability of the different extractants to remove organic carbon from aquifer sediments. The different extractants included pyrophosphate, sodium hydroxide, and polished water. Pyrophosphate served as a mild extractant that minimally alters the organic structure of the extracted material. Three concentrations (0.1, 0.5, and 1%) of pyrophosphate extracted 18.8, 24.9, and 30.8% of sediment organic carbon, respectively. Under alkali conditions (0.5 N NaOH), which provided the harshest extractant, 30.7% of the sediment organic carbon was recovered. Amorphous organic carbon, measured by potassium persulfate oxidization, consisted of 44.6% of the sediment organic carbon and served as a baseline control for maximum carbon removal. Conversely, highly purified water provided a minimal extraction control and extracted 5.7% of the sediment organic carbon. The removal of organic carbon was quantified by aqueous TOC in the extract and residual sediment organic carbon content. Characterization of the organic carbon extracts by compositional analysis prior and after exposure to the mixed culture might indicate the type organic carbon and functional groups used and/or generated by the organisms. This is an abstract of a paper presented at the 8th International In Situ and On-Site Bioremediation Symposium (Baltimore, MD 6/6-9/2005).

Conference Paper↗

Quantitative evaluation of XAD-8 and XAD-4 resins used in tandem for removing organic solutes from water

The combined XAD-8 and XAD-4 resin procedure for the isolation of dissolved organic solutes from water was found to isolate 85% or more of the organic solutes from Lake Skjervatjern in Norway. Approximately 65% of the dissolved organic carbon (DOC) was first removed on XAD-8 resin, and then an additional 20% of the DOC was removed on XAD-4 resin. Approximately 15% of the DOC solutes (primarily hydrophilic neutrals) were not sorbed or concentrated by the procedure. Of the 65% of the solutes removed on XAD-8 resin, 40% were fulvic acids, 16% were humic acids, and 9% were hydrophobic neutrals. Approximately 20% of the hydrophilic solutes that pass through the XAD-8 resin were sorbed solutes on the second resin, XAD-4 (i.e., they were hydrophobic relative to the XAD-4 resin). The fraction sorbed on XAD-4 resin was called XAD-4 acids because it represented approximately 85-90% of the hydrophilic XAD-8 acid fraction according to the original XAD-8 fractionation procedure. The recovery of hydrophobic acids (fulvic acids and humic acids) and the hydrophobic neutral fraction from XAD-8 resin was essentially quantitative at 96%, 98%, and 86%, respectively. The recovery of XAD-4 acids from the XAD-4 resin was only about 50%. The exact reason for this moderately low recovery is unknown, but could result from ??-?? bonding between these organic solutes and the aromatic matrix of XAD-4. The hydrophobic/hydrophilic solute separation on XAD-8 resin for water from background Side A and Side B of the lake was almost identical at 65 and 67%, respectively. This result suggested that both sides of the lake are similar in organic chemical composition even though the DOC variation from side to side is 20%.

Environment International↗

The Vigil Network

Those of us who seek to explain the variations in hydrologic phenomena such as may be observed in the occurrence of floods or in changes in the shape of river channels are painfully aware of the lack of adequate data. Our existing data, collected mainly to serve immediate practical needs for water-resources development, usually are deficient in providing information useful for many kinds of scientific inquiry. Hydrologic records are usually obtained on streams that are highly regulated or otherwise put to use, to the neglect of headwater streams better suited to studies of the details of hydrologic processes.

International Association of Scientific Hydrology ↗

Storm surges and climate change implications for tidal marshes: Insight from the San Francisco Bay Estuary, California, USA

Tidal marshes are dynamic ecosystems, which are influenced by oceanic and freshwater processes and daily changes in sea level. Projected sea-level rise and changes in storm frequency and intensity will affect tidal marshes by altering suspended sediment supply, plant communities, and the inundation duration and depth of the marsh platform. The objective of this research was to evaluate if regional weather conditions resulting in low-pressure storms changed tidal conditions locally within three tidal marshes. We hypothesized that regional storms will increase sea level heights locally, resulting in increased inundation of the tidal marsh platform and plant communities. Using site-level measurements of elevation, plant communities, and water levels, we present results from two storm events in 2010 and 2011 from the San Francisco Bay Estuary (SFBE), California, USA. The January 2010 storm had the lowest recorded sea level pressure in the last 30 years for this region. During the storm episodes, the duration of tidal marsh inundation was 1.8 and 3.1 times greater than average for that time of year, respectively. At peak storm surges, over 65% in 2010 and 93% in 2011 of the plant community was under water. We also discuss the implications of these types of storms and projected sea-level rise on the structure and function of the tidal marshes and how that will impact the hydro-geomorphic processes and marsh biotic communities.

California↗

Unique characteristics of the trachea of the juvenile leatherback turtle facilitate feeding, diving and endothermy

The adult leatherback turtle Dermochelys coriacea overlaps in body size (300–500 kg) with many marine mammals, yet develops from a 50 g hatchling. Adults can dive deeper than 1200 m and have core body temperatures of 25 °C; hatchlings are near-surface dwellers. Juvenile leatherbacks have rarely been studied; here we present anatomical information for the upper respiratory tract of 3 turtles (66.7–83.0 cm straight carapace length; 33.2–53.4 kg body mass) incidentally captured by long-line fisheries. Combined with existing information from adults and hatchlings, our data show that there is an ontogenic shift in tracheal structure, with cartilaginous rings becoming broader and eventually fusing anteriorly. This ontogenic shift during independent existence is unique among extant deep-diving air breathing vertebrates. Tract wall thickness is graded, becoming progressively thinner from larynx to bronchi. In addition, cross-sectional shape becomes increasingly dorsoventrally flattened (more elliptical) from anterior to posterior. These characteristics ensure that the tract will collapse from posterior to anterior during dives. This study contains the first report of a double (= internally bifurcated) posterior section of the trachea; it is suggested that this allows continuous food movement along the esophagus without tracheal collapse. The whole upper respiratory tract (from larynx to lungs) has a vascular lining (thicker anteriorly than posteriorly) that appears to be a simple analog of the complex turbinates of birds and mammals. Our study confirmed that the leatherback tracheal structure represents a distinctive way of dealing with the challenges of diving in deep, cold sea water.

Journal of Experimental Marine Biology and Ecology↗

Detection and attribution of temperature changes in the mountainous Western United States

Large changes in the hydrology of the western United States have been observed since the mid-twentieth century. These include a reduction in the amount of precipitation arriving as snow, a decline in snowpack at low and midelevations, and a shift toward earlier arrival of both snowmelt and the centroid (center of mass) of streamflows. To project future water supply reliability, it is crucial to obtain a better understanding of the underlying cause or causes for these changes. A regional warming is often posited as the cause of these changes without formal testing of different competitive explanations for the warming. In this study, a rigorous detection and attribution analysis is performed to determine the causes of the late winter/early spring changes in hydrologically relevant temperature variables over mountain ranges of the western United States. Natural internal climate variability, as estimated from two long control climate model simulations, is insufficient to explain the rapid increase in daily minimum and maximum temperatures, the sharp decline in frost days, and the rise in degree-days above 0??C (a simple proxy for temperature driven snowmelt). These observed changes are also inconsistent with the model-predicted responses to variability in solar irradiance and volcanic activity. The observations are consistent with climite simulations that include the combined effects of anthropogenic greenhouse gases and aerosols. It is found that, for each temperature variable considered, an anthropogenic signal is identifiable in observational fields. The results are robust to uncertainties in model-estimated fingerprints and natural variability noise, to the choice of statistical down-scaling method, and to various processing options in the detection and attribution method. ?? 2008 American Meteorological Society.

Journal of Climate↗

Hydrogeologic map of the Islamic Republic of Mauritania (phase V, deliverable 56), Synthesis of hydrologic data (phase V, deliverable 57), and chemical hydrologic map of the Islamic Republic of Mauritania (added value)

A hydrogeologic study was conducted to support mineral-resource assessment activities in Mauritania, Africa. Airborne magnetic depth estimates reveal two primary groundwater basins: the porous coastal Continental Terminal Basin (fill deposits); and the interior, fractured interior Taoudeni Basin. In the Continental Terminal Basin, there is uniform vertical recharge and localized discharge that is coincident with groundwater pumping at Nouakchott. This pumping center induces eastward flow of groundwater from the Atlantic Ocean resulting in a salinity gradient that diminishes quality over 100 km. Groundwater also flows southward into the basin from Western Sahara. By contrast, an interbasin exchange occurs as fresh groundwater flows westward from the Taoudeni Basin. In the Taoudeni Basin, zones of local recharge occur in three areas: northwest at the edge of the Rgueïbat Shield; at the city of Tidjikja; and near the center of the basin. Groundwater also flows across international boundaries: northward into Western Sahara and westward into Mali. At the southern country boundary, the Senegal River serves as both a source and sink of fresh groundwater to the Continental Terminal and Taoudeni basins. Using a geographical information system, thirteen hydrogeologic units are identified based on lateral extent and distinct hydraulic properties for future groundwater model development. Combining this information with drilling productivity, groundwaterquality, and geophysical interpretations (fracturing and absence of subsurface dikes) three potential water-resource development targets were identified: sedimentary rocks of the Jurassic, Cretaceous, and Quaternary Periods; sedimentary rocks of Cambrian and Ordovician Periods; and sedimentary rocks of Neoproterozoic age.

Open-File Report↗

Distribution and ecology of Dreissena polymorpha (pallas) and Dreissena bugensis (andrusov) in the upper Volga basin

This paper presents data on contemporary distribution patterns of two species of Dreissenidae, the zebra mussel (Dreissena polymorpha) and the Quagga mussel (Dreissena bugensis), and their role in ecosystem processes in the Ivan'kovo, Uglich, Rybinsk, and Gorky Reservoirs of the Upper Volga River basin. The role of zebra mussel was also studied in experimental mesocosms of 15 m3. Maximum abundance and species diversity of macroinvertebrates, especially of leeches, polychaetes, crustaceans, and heterotopic insects, were attained in the portions of reservoirs where Dreissenidae were present and in experimental mesocosms where zebra mussel biomass was the highest. In the mesocosm studies, the presence of zebra mussel druses (colonies) provided shelter for macroinvertebrates, reducing their vulnerability to predation by perch (Perca fluviatills) larvae and yearlings, thereby increasing macroinvertebrate species diversity. It was shown that in addition to its role in aquatic biocenosis (ecological community) formation and water purification, Dreissenidae are important food objects for benthophagous fishes, especially roach (Rutilus rutilus). Examination of intestines of benthophagous fishes showed that the length of Dreissenidae ranged from 5 to 20 mm in roach; from 4 to 14 mm in silver bream (Blicca bjoerkna), and from 2 to 10 mm in bream (Abramis brama). The largest mussels consumed were Quagga mussels up to 30 mm, noted in the predatory cyprinid, ide (Leuciscus idus). Copyright ?? 2006 by ASTM International.

Journal of ASTM International↗

Molecular sequences derived from Paleocene Fort Union Formation coals vs. associated produced waters: Implications for CBM regeneration

Coalbed methane regeneration is of increasing interest, and is gaining global attention with respect to enhancement of gas recovery. The objective of this study is to determine if there are differences in methanogen nucleic acid sequences associated with low rank coals from the Powder River Basin, Wyoming, in comparison with sequences that can be recovered from coal bed-associated produced waters. Based on results obtained to date, the sequences from the coals appear to be associated with putatively deep-rooted thermophilic autotrophic methanogens, whereas the sequences from the waters are associated with thermophilic autotrophic and heterotrophic methanogens. The recovered sequences associated with coal thus appear to be both phylogenetically and functionally distinct from those that are more closely associated with the produced water. To be able to relate such recovered sequences to organisms that might be present and possibly active in these environments, it is suggested that direct observation, followed by isolation and single cell-based physiological/molecular analyses, be used to characterize methanogenic consortia possibly associated with coals and/or produced waters. It is also important to characterize the microenvironment where these microbes might be found, in both ecological and geological contexts, to be able to develop effective, ecologically relevant coalbed methane regeneration processes.

International Journal of Coal Geology↗

Are we heading towards a global decrease in coregonine catches?

Coregonines have specific ecological needs, making them potentially very vulnerable to changes in lake conditions. A contemporary concern is that many lakes worldwide are experiencing environmental changes due to anthropogenic pressure and climate warming. Here, we compiled long-term data of coregonine catches from 27 lakes from three continents in the northern hemisphere. Declines in catch were observed in 67% of the lakes during the first two decades of the 21 st century, with a significant trend (p-value<0.05) in 44% of the cases. An analysis to determine whether trends are globally linked to environmental conditions and specific lake attributes was carried out on 26 lakes for the period 2000–2019. Several local declines in catches had already been documented in the literature and are likely to be due to local forcing such as nutrients, species invasions and changes in fishing practices. Nevertheless, on a global scale, our results indicate that lakes, which exhibited a significant decrease in catch were larger and more nutrient-poor than other lakes. The rate of change in catches appeared to be related to the trophic state of the lake. The specific effect of warming is difficult to determine during the studied period. When warming occurred outside the period of egg incubation, decreasing trends were more frequently observed in nutrient-poor than in mesotrophic lakes. In conclusion, our findings suggest that achieving oligo- or ultraoligotrophic conditions, as required in developed countries to control phytoplankton blooms and enhance water quality, could pose significant challenges for future management of coregonine fisheries.

International Journal of Limnology↗

Flat-plate techniques for measuring reflectance of macro-algae ( Ulva curvata )

We tested the consistency and accuracy of flat-plate spectral measurements (400&ndash;1000 nm) of the marine macrophyte Ulva curvata . With sequential addition of Ulva thallus layers, the reflectance progressively increased from 6% to 9% with six thalli in the visible (VIS) and from 5% to 19% with ten thalli in the near infrared (NIR). This progressive increase was simulated by a mathematical calculation based on an Ulva thallus diffuse reflectance weighted by a transmittance power series. Experimental and simulated reflectance differences that were particularly high in the NIR most likely resulted from residual water and layering structure unevenness in the experimental progression. High spectral overlap existed between fouled and non-fouled Ulva mats and the coexistent lagoon mud in the VIS, whereas in the NIR, spectral contrast was retained but substantially dampened by fouling.

International Journal of Remote Sensing↗

Analysis of vegetation recovery surrounding a restored wetland using the normalized difference infrared index (NDII) and normalized difference vegetation index (NDVI)

Watershed restoration efforts seek to rejuvenate vegetation, biological diversity, and land productivity at Cienega San Bernardino, an important wetland in southeastern Arizona and northern Sonora, Mexico. Rock detention and earthen berm structures were built on the Cienega San Bernardino over the course of four decades, beginning in 1984 and continuing to the present. Previous research findings show that restoration supports and even increases vegetation health despite ongoing drought conditions in this arid watershed. However, the extent of restoration impacts is still unknown despite qualitative observations of improvement in surrounding vegetation amount and vigor. We analyzed spatial and temporal trends in vegetation greenness and soil moisture by applying the normalized difference vegetation index (NDVI) and normalized difference infrared index (NDII) to one dry summer season Landsat path/row from 1984 to 2016. The study area was divided into zones and spectral data for each zone was analyzed and compared with precipitation record using statistical measures including linear regression, Mann– Kendall test, and linear correlation. NDVI and NDII performed differently due to the presence of continued grazing and the effects of grazing on canopy cover; NDVI was better able to track changes in vegetation in areas without grazing while NDII was better at tracking changes in areas with continued grazing. Restoration impacts display higher greenness and vegetation water content levels, greater increases in greenness and water content through time, and a decoupling of vegetation greenness and water content from spring precipitation when compared to control sites in nearby tributary and upland areas. Our results confirm the potential of erosion control structures to affect areas up to 5 km downstream of restoration sites over time and to affect 1 km upstream of the sites.

Cuenca Los Ojos, San Bernardino National Wildlife ↗

Depth calibration of the Experimental Advanced Airborne Research Lidar, EAARL-B

Introduction The original National Aeronautics and Space Administration (NASA) Experimental Advanced Airborne Research Lidar (EAARL) was extensively modified to increase the spatial sampling density and to improve performance in water ranging from 3 to 44 meters (m). The new (EAARL-B) sensor features a higher spatial density that was achieved by optically splitting each laser pulse into three pulses spatially separated by 1.6 m along the flight track and 2.0 m across the flight track, on the water surface when flown at a nominal altitude of 300 m (984 feet). The sample spacing can be optionally increased to 1.0 m across the flight track. Improved depth capability was achieved by increasing the total peak laser power by a factor of 10 and by designing a new &ldquo;deep-water&rdquo; receiver, which is optimized to exclusively receive refracted and scattered light from deeper water (15&ndash;44 m). Two different clear-water flight missions were conducted over the U.S. Navy's South Florida Testing Facility (SFTF) to determine the EAARL-B calibration coefficients. The SFTF is an established lidar calibration range located in the coastal waters southeast of Fort Lauderdale, Florida. We used 23 selected polygons at 23 distinct depths to compare a reference dataset from this site to determine EAARL-B calibration constants over the depth range of 6.5 to 34 m. We also conducted a near-simultaneous single-beam jet-ski-based sonar survey of selected transects ranging from 1 to 33 m depth in the same area. The near-concurrent jet ski data were used to evaluate the EAARL-B performance over the depth range from 0.9 to 10 m. The more timely jet ski data were necessary because the primary reference dataset was 9 years old, and areas shallower than 6.5 m are dominated by shifting sand. We determined the jet ski data were not useful as a calibration reference in water deeper than 10 m due to large uncertainty in the vertical measurement introduced by the lack of any sensor orientation data, that is, for pitch, roll, and heading to correct the measured slant range to a vertical measurement. The resulting calibrated EAARL-B data were then analyzed and compared with the original reference dataset, the jet-ski-based dataset from the same Fort Lauderdale site, as well as the depth-accuracy requirements of the International Hydrographic Organization (IHO). We do not claim to meet all of the IHO requirements and standards. The IHO minimum depth-accuracy requirements were used as a reference only and we do not address the other IHO requirements such as &ldquo; Full Seafloor Search&rdquo;. Our results show good agreement between the calibrated EAARL-B data and all reference datasets, with results that are within the 95 percent depth accuracy of the IHO Order 1 (a and b) depth-accuracy requirements.

Open-File Report↗

Trace silicon determination in biological samples by inductively coupled plasma mass spectrometry (ICP-MS): Insight into volatility of silicon species in hydrofluoric acid digests for optimal sample preparation and introduction to ICP-MS

A method for the determination of trace levels of silicon from biological materials by inductively coupled plasma mass spectrometry (ICP-MS) has been developed. The volatility of water-soluble silicon species, hexafluorosilicic acid (H 2 SiF 6 ), and sodium metasilicate (Na 2 SiO 3 ) was investigated by evaporating respective solutions (50 µg/mL silicon) in nitric acid (HNO 3 ), nitric acid + hydrochloric acid (HNO 3 + HCl), and nitric acid + hydrochloric acid + hydrofluoric acid (HNO 3 + HCl + HF) at 120 °C on a hot-block to near dryness. The loss of silicon from H 2 SiF 6 solutions was substantial (>99%) regardless of the digestion medium. Losses were also substantial (>98%) for metasilicate solutions heated in HNO 3 + HCl + HF, while no significant loss occurred in HNO 3 or HNO 3 + HCl. These results show that H 2 SiF 6 species were highly volatile and potential losses could confound accuracy at trace level determinations by ICP-MS if digestates prepared in HF are heated to eliminate HF. Among the various matrices comprising major elements, sodium appeared to be effective in reducing silicon loss from H 2 SiF 6 solutions. Excess sodium chloride (NaCl) matrix provided better stability, improving silicon recoveries by up to about 80% in evaporated HF digestates of soil and mine waste samples, but losses could not be fully prevented. To safely remove excess acids and circumvent the adverse effects of excess HF (e.g., risk of high Si background signals), a two-step digestion scheme was adopted for the preparation of biological samples containing trace silicon levels. A closed-vessel digestion was performed either in 4 mL of concentrated HNO 3 and 1 mL of concentrated HCl or 4 mL of concentrated HNO 3 , 1 mL of concentrated HCl and 1 mL of concentrated HClO 4 on a hot plate at 140 °C. Digestates were then evaporated to incipient dryness at 120 °C to remove the acids. A second closed-vessel digestion was carried out to dissolve silicates in 0.5 mL of concentrated HNO 3 and 0.5 mL of concentrated HF at 130 °C. After digestion, digestates were diluted to 10 mL. The solution containing about 5% HNO 3 and 5% HF was directly analyzed by ICP-MS equipped with an HF-inert sample introduction system. The limit of detection was about 110 µg/L for 28 Si when using the Kinetic Energy Discrimination (KED) mode. The method was used to determine silicon in various plant and tissue certified reference materials. Data were acquired for 28 Si using KED and standard (STD) modes, and 74 Ge and 103 Rh as internal standard elements. There was not any significant difference between the accuracy and precision of the results obtained with 74 Ge and 103 Rh within the same measurement mode. Precision, calculated as relative standard deviation for four replicate analyses, varied from 5.3 (tomato leaves) to 21% (peach leaves) for plant and from 2.2 (oyster tissue) to 33% (bovine liver) for tissue SRM/CRMs. Poor precision was attributed to material heterogeneity and the large particle size distribution. An analysis of lung tissue samples from those with occupational exposure to silica dust revealed that tissues possessed substantial levels of water-soluble silicates, but the most silicon was present in the particulate matter fraction.

Minerals↗

A comparison of the chemical sensitivities between in vitro and in vivo propagated juvenile freshwater mussels: Implications for standard toxicity testing

Unionid mussels are ecologically important and are globally imperiled. Toxicants contribute to mussel declines, and toxicity tests using juvenile mussels—a sensitive life stage—are valuable in determining thresholds used to set water quality criteria. In vitro culture methods provide an efficient way to propagate juveniles for toxicity testing, but their relative chemical sensitivity compared with in vivo propagated juveniles is unknown. Current testing guidelines caution against using in vitro cultured juveniles until this sensitivity is described. Our objective was to evaluate the relative sensitivity of juvenile mussels produced from both in vitro and in vivo propagation methods to selected chemicals. We conducted 96-h acute toxicity tests according to ASTM International guidelines with 3 mussel species and 6 toxicants: chloride, nickel, ammonia, and 3 copper-based compounds. Statistically significant differences between in vitro and in vivo juvenile 96-h median effect concentrations were observed in 8 of 17 tests, and in vitro juveniles were more sensitive in 6 of the 8 significant differences. At 96 h, 4 of the 8 statistically different tests for a given chemical were within a factor of 2, which is the intralaboratory variation demonstrated in a recent evaluation of mussel toxicity tests. We found that although differences in chemical sensitivity exist between in vitro and in vivo propagated juvenile mussels, they are within normal toxicity test variation. Therefore, in vitro propagated juvenile mussels may be appropriate for use in ASTM International-based toxicity testing.

Environmental Toxicology and Chemistry↗

Environmental regulation and influence of the eyes and pineal gland on the gonadal cycle and spawning in channel catfish (Ictalurus punctatus)

Blinded, pinealectomized, or blinded and pinealectomized female channel catfish ( Ictalurus punctatus ) were placed with normal (unoperated on) control fish in outdoor tanks at constant temperature (21 ± 2 C) or earthen ponds (ambient conditions) in February or August, when they were 21 or 27 mo old. Fish were sampled through the following reproductive season. The gonadosomatic index (GSI) and plasma estrogen concentration changed seasonally in 3-yr-old fish, but the changes were less marked or lacking in 2-yr-olds. The GSI levels of sexually mature (≥3 years old) fish peaked about a month earlier in the tanks than in the ponds; the estrogen peak for fish in the tanks was lower than that for pond fish. All experimental fish (both ages) in ponds delayed ovarian resorption for about 1 mo, compared with normal control fish. Exposure to constant 21 C water allowed earlier gonadal recrudescence. All groups of sexually mature fish that were surgically altered in August spawned during the following spring. Fish with eyes spawned earlier and had higher spawning percentages (control 68%, pinealectomized 75%) than did fish without eyes (blinded 50%, blinded and pinealectomized 56%). Neither the eyes nor the pineal is essential for spring gonadal maturation or for spawning in channel catfish, but one or both may have a role in timing these events. An annual internal oscillator that may be modified by environmental temperature is suggested as the primary control of reproductive cycling in the species. Light information obtained through the pineal, eyes, or both appears to affect the time of gonadal resorption.

Ecological and Evolutionary Physiology↗

Monthly fractional green vegetation cover associated with land cover classes of the conterminous USA

The land cover classes developed under the coordination of the International Geosphere-Biosphere Programme Data and Information System (IGBP-DIS) have been analyzed for a study area that includes the Conterminous United States and portions of Mexico and Canada. The 1-km resolution data have been analyzed to produce a gridded data set that includes within each 20-km grid cell: 1) the three most dominant land cover classes, 2) the fractional area associated with each of the three dominant classes, and 3) the fractional area covered by water. Additionally, the monthly fraction of green vegetation cover (fgreen) associated with each of the three dominant land cover classes per grid cell was derived from a 5-year climatology of 1-km resolution NOAA-AVHRR data. The variables derived in this study provide a potential improvement over the use of monthly fgreen linked to a single land cover class per model grid cell.

Geophysical Research Letters↗