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Pesticide toxicity index for freshwater aquatic organisms

The U.S. Geological Survey's National Water-Quality Assessment (NAWQA) Program is designed to assess current water-quality conditions, changes in water quality over time, and the effects of natural and human factors on water quality for the Nation's streams and ground-water resources. For streams, one of the most difficult parts of the assessment is to link chemical conditions to effects on aquatic biota, particularly for pesticides, which tend to occur in streams as complex mixtures with strong seasonal patterns. A Pesticide Toxicity Index (PTI) was developed that combines pesticide exposure of aquatic biota (measured concentrations of pesticides in stream water) with toxicity estimates (standard endpoints from laboratory bioassays) to produce a single index value for a sample or site. The development of the PTI was limited to pesticide compounds routinely measured in NAWQA studies and to toxicity data readily available from existing databases. Qualifying toxicity data were found for one or more types of test organisms for 75 of the 83 pesticide compounds measured in NAWQA samples, but with a wide range of bioassays per compound (1 to 65). There were a total of 2,824 bioassays for the 75 compounds, including 287 48-hour EC50 values (concentration at which 50 percent of test organisms exhibit a nonlethal response) for freshwater cladocerans, 585 96-hour LC50 values (concentration lethal to 50 percent of test organisms) for freshwater benthic invertebrates, and 1,952 96-hour LC50 values for freshwater fish. The PTI for a particular sample is the sum of toxicity quotients (measured concentration divided by the median toxicity concentration from bioassays) for each detected pesticide. The PTI can be calculated for specific groups of pesticides and for specific taxonomic groups.While the PTI does not determine whether water in a sample is toxic, its values can be used to rank or compare the toxicity of samples or sites on a relative basis for use in further analysis or additional assessments. The PTI approach may be useful as a basis for comparing the potential significance of pesticides in different streams on a common basis, for evaluating relations between pesticide exposure and observed biological conditions, and for prioritizing where further studies are most needed.

Water-Resources Investigations Report↗

Incubation temperature, developmental biology, and the divergence of sockeye salmon ( Oncorhynchus nerka ) within Lake Washington

Sockeye salmon ( Oncorhynchus nerka) introduced into Lake Washington in the 1930s and 1940s now spawn at several different sites and over a period of more than 3 months. To test for evolutionary divergence within this derived lineage, embryos that would have incubated in different habitats (Cedar River or Pleasure Point Beach) or at different times (October, November, or December in the Cedar River) were reared in the laboratory at 5, 9, and 12.5??C. Some developmental variation mirrored predictions of adaptive divergence: (i) survival at 12.5??C was highest for embryos most likely to experience such temperatures in the wild (Early Cedar), (ii) development rate was fastest for progeny of late spawners (Late Cedar), and (iii) yolk conversion efficiency was matched to natural incubation temperatures. These patterns likely had a genetic basis because they were observed in a common environment and could not be attributed to differences in egg size. The absolute magnitude of divergence in development rates was moderate (Late Cedar embryos emerged only 6 days earlier at 9??C) and some predictions regarding development rates were not supported. Nonetheless our results provide evidence of adaptive divergence in only 9-14 generations.

Washington↗

Effects of aquifer storage and recovery activities on water quality in the Little Arkansas River and Equus Beds Aquifer, south-central Kansas, 2011–14

The Equus Beds aquifer in south-central Kansas is aprimary water source for the city of Wichita. The Equus Beds aquifer storage and recovery (ASR) project was developed to help the city of Wichita meet increasing current (2016) and future water demands. The Equus Beds ASR project pumps water out of the Little Arkansas River during above-base flow conditions, treats it using drinking-water quality standards as a guideline, and recharges it into the Equus Beds aquifer for later use. Phase II of the Equus Beds ASR project currently (2016) includes a river intake facility and a surface-water treatment facility with a 30 million gallon per day capacity. Water diverted from the Little Arkansas River is delivered to an adjacent presedimentation basin for solids removal. Subsequently, waste from the surface-water treatment facility and the presedimentation basin is returned to the Little Arkansas River through a residuals return line. The U.S. Geological Survey, in cooperation with the city of Wichita, developed and implemented a hydrobiological monitoring program as part of the ASR project to characterize and quantify the effects of aquifer storage and recovery activities on the Little Arkansas River and Equus Beds aquifer water quality. Data were collected from 2 surface-water sites (one upstream and one downstream from the residuals return line), 1 residuals return line site, and 2 groundwater well sites (each having a shallow and deep part): the Little Arkansas River upstream from the ASR facility near Sedgwick, Kansas (upstream surface-water site 375350097262800), about 0.03 mile (mi) upstream from the residuals return line site; the Little Arkansas River near Sedgwick, Kans. (downstream surface-water site 07144100), about 1.68 mi downstream from the residuals return line site; discharge from the Little Arkansas River ASR facility near Sedgwick, Kansas (residuals return line site 375348097262800); 25S 01 W 07BCCC01 SMW–S11 near CW36 (MW–7 shallow groundwater well site 375327097285401); 25S01 W 07BCCC02 DMW–S10 near CW36 (MW–7 deep groundwater well site 375327097285402); 25S 01W 07BCCA01 SMW–S13 near CW36 (MW–8 shallow groundwater well site 375332097284801); and 25S 01W 07BCCA02 DMW–S14 near CW36 (MW–8 deep groundwater well site 375332097284802). The U.S. Geological Survey, in cooperation with the city of Wichita, assessed the effects of the ASR Phase II facility residuals return line discharges on stream quality of the Little Arkansas River by measuring continuous physicochemical properties and collecting discrete water-quality and sediment samples for about 2 years pre- (January 2011 through April 2013) and post-ASR (May 2013 through December 2014) Phase II facility operation upstream and downstream from the ASR Phase II facility. Additionally, habitat variables were quantified and macroinvertebrate and fish communities were sampled upstream and downstream from the ASR Phase II facility during the study period. To assess the effects of aquifer recharge on Equus Beds groundwater quality, continuous physicochemical properties were measured and discrete water-quality samples were collected before and during the onset of Phase II aquifer recharge in two (shallow and deep) groundwater wells. Little Arkansas River streamflow was about 10 times larger after the facility began operating because of greater rainfall. Residuals return line release volumes were a very minimal proportion (0.06 percent) of downstream streamflow volume during the months the ASR facility was operating. Upstream and downstream continuously measured water temperature and dissolved oxygen median differences were smaller post-ASR than pre-ASR. Turbidity generally was smaller at the downstream site throughout the study period and decreased at both sites after the ASR Phase II facility began discharging despite a median residuals return line turbidity that was about an order of magnitude larger than the median turbidity at the downstream site. Upstream and downstream continuously measured turbidity median differences were larger post-ASR than pre-ASR. Median post-ASR continuously measured nitrite plus nitrate and continuously computed total suspended solids and suspended-sediment concentrations were smaller than pre-ASR likely because of higher streamflows and dilution; whereas, median continuously computed dissolved and total organic carbon concentrations were larger likely because of higher streamflows and runoff conditions. None of the discretely measured water-quality constituents (dissolved and suspended solids, primary ions, suspended sediment, nutrients, carbon, trace elements, viral and bacterial indicators, and pesticides) in surface water were significantly different between the upstream and downstream sites after the ASR Phase II facility began discharging; however, pre-ASR calcium, sodium, hardness, manganese, and arsenate concentrations were significantly larger at the upstream site, which indicates that some water-quality conditions at the upstream and downstream sites were more similar post-ASR. Most of the primary constituents that make up dissolved solids decreased at both sites after the ASR Phase II facility began operation. Discretely collected total suspended solids concentrations were similar between the upstream and downstream sites before the facility began operating but were about 27 percent smaller at the downstream site after the facility began operating, despite the total suspended solids concentrations in the residuals return line being 15 times larger than the downstream site. Overall habitat scores were indicative of suboptimal conditions upstream and downstream from the ASR Phase II facility throughout the study period. Substrate fouling and sediment deposition mean scores indicated marginal conditions at the upstream and downstream sites during the study period, demonstrating that sediment deposition was evident pre- and post-ASR and no substantial changes in these habitat characteristics were noted after the ASR Phase II facility began discharging. Macroinvertebrate community composition (evaluated using functional feeding, behavioral, and tolerance metrics) generally was similar between sites during the study period. Fewer macroinvertebrate metrics were significant between the upstream and downstream sites post-ASR (6) than pre-ASR (14), which suggests that macroinvertebate communities were more similar after the ASR facility began discharging. Upstream-downstream comparisons in macroinvertebrate aquatic-life-support metrics had no significant differences for the post-ASR time period and neither site was fully supporting for any of the Kansas Department of Health and Environment aquatic-life-support metrics (Macroinvertebrate Biotic Index; Kansas Biotic Index with tolerances for nutrients and oxygen-demanding substances; Ephemeroptera, Plecoptera, and Trichoptera [EPT] richness; and percentage of EPT species). Overall, using macroinvertebrate aquatic life-support criteria from the Kansas Department of Health and Environment, upstream and downstream sites were classified as partially supporting before and after the onset of ASR facility operations. Fish community trophic status and tolerance groups generally were similar among sites during the study period. Fish community Little Arkansas River Basin Index of Biotic Integrity scores at the upstream and downstream sites were indicative of fair-to-good conditions before the facility began operating and decreased to fair conditions after the facility began operating. Groundwater physicochemical changes concurrent with the beginning of recharge operations at the Sedgwick basin were more pronounced in shallow groundwater. No constituent concentrations in the pre-recharge period in comparison to the post-recharge period increased to concentrations exceeding drinking water regulations; however, nitrate decreased significantly from a pre-recharge exceedance of the U.S. Environmental Protection Agency maximum contaminant level to a post recharge nonexceedance. Shallow groundwater chemical concentrations or rates of detection increased after artificial recharge began for the ions potassium, chloride, and fluoride; phosphorus and organic carbon species; trace elements barium, manganese, nickel, arsenate, arsenic, and boron; agricultural pesticides atrazine, metolachlor, metribuzin, and simazine; organic disinfection byproducts bromodichloromethane and trichloromethane; and gross beta levels. Additionally, water temperature, and pH were larger after recharge began; and total solids and slime-forming bacteria concentrations and densities were smaller. Total solids, nitrate, and selenium significantly decreased; and potassium, chloride, nickel, arsenic, fluoride, phosphorus and carbon species, and gross beta levels significantly increased in shallow groundwater after artificial recharge. Results of biological activity reaction tests indicated that water quality microbiology was different before and after artificial recharge began; at times, these differences may lead to changes in dominant bacterial populations that, in turn, may lead to formation and expansion in populations that may cause bioplugging and other unwanted effects. Calcite, iron (II) hydroxide, hydroxyapatite, and similar minerals, had shifts in saturation indices that generally were from undersaturation toward equilibrium and, in some cases, toward oversaturation. These shifts toward neutral saturation indices might suggest reduced weathering of the minerals present in the Equus Beds aquifer. Chemical weathering in the shallow parts of the aquifer may be accelerated because of the increased water temperatures and the system is more vulnerable to clogged pores and mineral dissolution as the equilibrium state is affected by recharge and withdrawal. When oversaturation is indicated for iron minerals, plugging of aquifer materials may happen.

Kansas↗

An empirically based simulation model to inform flow management for endangered species conservation

Increasing water demand, water development, and ongoing climate change have driven extensive changes to the hydrology, geomorphology and biology of arid-land rivers globally, driving an increasing need to understand how annual hydrologic conditions affect the distribution and abundance of imperiled desert fish populations. We analyzed the relationship between annual hydrologic conditions and the endangered Rio Grande silvery minnow ( Hybognathus amarus ) in the Middle Rio Grande, New Mexico, USA, using hurdle models to predict both presence and density as a function of integrated annual hydrologic metrics. Both presence and density were positively related to spring high flow magnitude and duration and negatively related to summer drying, as indicated by an integrated flow metric. Simulations suggest hydrologic conditions near the wettest observed in the data set would be required to meet recovery goals in a single year in all reaches. We demonstrate how the models developed herein can be used to examine alternative water management strategies, including strategies that may currently be socially and logistically infeasible to implement, to identify strategies minimizing trade-offs between conservation and other management goals.

New Mexico↗

Flow-ecology relationships are spatially structured and differ among flow regimes

In streams, hydrology is a predominant driver of ecological structure and function. Providing adequate flows to support aquatic life, or environmental flows, is therefore a top management priority in stream systems. Flow regime classification is a widely accepted approach for establishing environmental flow guidelines. However, it is surprisingly difficult to quantify relationships between hydrology and ecology (flow–ecology relationships) while describing how these relationships vary across classified flow regimes. Developing such relationships is complicated by several sources of spatial bias, such as autocorrelation due to spatial design, flow regime classification and other environmental or ecological sources of spatial bias. We used mixed moving‐average spatial stream network models to develop flow–ecology relationships across classified flow regimes and to assess spatial patterns of these relationships. We compared relationships between fish traits and life‐history strategies with hydrologic metrics across flow regimes and assessed whether spatial autocorrelation influenced these relationships. Trait–hydrology relationships varied between flow regimes and across all streams combined. Some relationships between traits and hydrologic metrics fit predictions based on life‐history theory, while others exhibited unexpected relationships with hydrology. Spatial factors described a large proportion of variability in fish traits and different patterns of spatial autocorrelation were observed in different flow regimes. Synthesis and applications. Further work is needed to understand why flow–ecology relationships vary across classified flow regimes and why these relationships may not fit predictions based on life‐history theories. Managers determining environmental flow standards need to be aware that different hydrologic metrics are often important drivers of fish trait diversity in different flow regimes. Flow–ecology relationships may therefore be confounded by spatial structure inherent in flow regime classification and much existing biological data. Complex patterns of spatial bias should be considered when managing stream systems within an environmental flows framework.

Journal of Applied Ecology↗

Ecological impacts of non-native species

Non-native species are considered one of the greatest threats to freshwater biodiversity worldwide (Drake et al. 1989; Allen and Flecker 1993; Dudgeon et al. 2005). Some of the first hypotheses proposed to explain global patterns of amphibian declines included the effects of non-native species (Barinaga 1990; Blaustein and Wake 1990; Wake and Morowitz 1991). Evidence for the impact of non-native species on amphibians stems (1) from correlative research that relates the distribution or abundance of a species to that of a putative non-native species, and (2) from experimental tests of the effects of a non-native species on survival, growth, development or behaviour of a target species (Kats and Ferrer 2003). Over the past two decades, research on the effects of non-native species on amphibians has mostly focused on introduced aquatic predators, particularly fish. Recent research has shifted to more complex ecological relationships such as influences of sub-lethal stressors (e.g. contaminants) on the effects of non-native species (Linder et al. 2003; Sih et al. 2004), non-native species as vectors of disease (Daszak et al. 2004; Garner et al. 2006), hybridization between non-natives and native congeners (Riley et al. 2003; Storfer et al. 2004), and the alteration of food-webs by non-native species (Nystrom et al. 2001). Other research has examined the interaction of non-native species in terms of facilitation (i.e. one non-native enabling another to become established or spread) or the synergistic effects of multiple non-native species on native amphibians, the so-called invasional meltdown hypothesis (Simerloff and Von Holle 1999). Although there is evidence that some non-native species may interact (Ricciardi 2001), there has yet to be convincing evidence that such interactions have led to an accelerated increase in the number of non-native species and cumulative impacts are still uncertain (Simberloff 2006). Applied research on the control, eradication, and management of non-native species has only just begun but some promising results have already emerged (see below), giving hope to a very difficult conservation issue. This chapter provides an overview of the role of non-native species in amphibian declines and summarizes the current state of knowledge of non-native species that are known, or considered to be, a threat to amphibian species and populations. The biological and socio-economic issues of non-native species control are examined and brief case studies of successful eradication programmes are provided.

Book chapter↗

Results of an adaptive environmental assessment modeling workshop concerning potential impacts of drilling muds and cuttings on the marine environment

Drilling fluids or "muds" are essential components of modern drilling operations. They provide integrity for the well bore, a medium for removal of formation cuttings, and lubrication and cooling of the drill bit and pipe. The modeling workshop described in this report was conducted September 14-18, 1981 in Gulf Breeze, Florida to consider potential impacts of discharged drilling muds and cuttings on the marine environment. The broad goals of the workshop were synthesis of information on fate and effects, identification of general relationships between drilling fluids and the marine environment, and identification of site-specific variables likely to determine impacts of drilling muds and cuttings in various marine sites. The workshop was structured around construction of a model simulating fate and effects of discharges from a single rig into open water areas of the Gulf of Mexico, and discussion of factors that might produce different fate and effects in enclosed areas such as bays and estuaries. The simulation model was composed of four connected submodels. A Discharge/Fate submodel dealt with the discharge characteristics of the rig and the subsequent fate of discharged material. Three effects submodels then calculated biological responses at distances away from the rig for the water column, soft bottom benthos (assuming the rig was located over a soft bottom environment), and hard bottom benthos (assuming the rig was located over a hard bottom environment). The model focused on direct linkages between the discharge and various organisms rather than on how the marine ecosystem itself is interconnected. Behavior of the simulation model indicated relatively localized effects of drilling muds and cuttings discharged from a single platform into open water areas. Water column fate and effects were dominated by rapid dilution. Effects from deposition of spent mud and cuttings were spatially limited with relatively rapid recovery, especially in soft bottom benthic communities which were conceptualized as being adapted to frequent storms. This behavior was generated by the set of assumptions about linkages and functional relationships used to construct the model. Areas of uncertainty included methods for extrapolating 96-hr LC50 so results to exposures of varying lengths and concentrations; recovery rates of benthic communities; responses to various depths and rates of burial; fate and effects of the plume in relationship to stratification layers; and long-term and sub-lethal effects of slightly elevated concentrations of discharged materials. Evaluation of the assumptions of the Soft Bottom Submodel suggest that the assumptions used may have been relatively liberal estimates of resiliency of these communities. Discussion of "closed" water bodies such as bays and estuaries indicated several reasons to expect different and more complex fate and effects behavior in these areas. These factors included different species and communities (such as aquatic macrophytes and oyster beds), more complex circulation and stratification patterns, and potentially more active resuspension processes. Much of the possible difference in behavior in these areas centers around the extent to which they are “closed” or in the relative residence times of water and sediments in these areas as they determine the long-term dispersion of discharged material. Despite the complexity and variability of these areas, a large body of knowledge (such as that concerning fate and physical effects of dredge spoil) that could be effectively employed in analysis of potential fate and physical effects in enclosed areas was identified.

Report↗

What can conservation culturomics tell us about factors driving public interest in aquatic endangered species

The field of “Conservation Culturomics” uses large datasets of freely available web-data to understand cultural patterns and public interests related to conservation topics. We used a popular culturomics tool based on search engine usage to investigate how the U.S. Endangered Species Act listing actions may influence public interest in imperiled freshwater taxa. Yet questions remain regarding the acceptable applications of these data leading us to also evaluate aspects of data quality such as repeatability of timeseries and spatial relative search volume (RSV) data for our search terms. We discovered that low search volume for many freshwater species restricted the number of species that could be analyzed and may signal low public awareness for these species. Low repeatability of timeseries relative search volume data suggests that greater scrutiny and quality control methods may be needed when analyzing these data. For species that had the highest data repeatability, there were positive associations of listing actions and relative search volume for threatened and endangered species. Anomalous peaks of search volume were sometimes but not always related to listing actions or online news stories. Spatial analysis indicated that search volumes were highest in states within species’ native ranges suggesting that people are most interested in species that occur near where they live.

Biological Conservation↗

Characterizing fish community diversity across Virginia landscapes: Prerequisite for conservation

The number of community types occurring within landscapes is an important, but often unprotected, component of biological diversity. Generally applicable protocols for characterizing community diversity need to be developed to facilitate conservation. We used several multivariate techniques to analyze geographic variation in the composition of fish communities in Virginia streams. We examined relationships between community composition and six landscape variables: drainage basin, physiography, stream order, elevation, channel slope, and map coordinates. We compared patterns at two scales (statewide and subdrainage-specific) to assess sensitivity of community classification to spatial scale. We also compared patterns based on characterizing communities by species composition vs. ecological composition. All landscape variables explained significant proportions of the variance in community composition. Statewide, they explained 32% of the variance in species composition and 48% of the variance in ecological composition. Typical communities in each drainage or physiography were statistically distinctive. Communities in different combinations of drainage, physiography, and stream size were even more distinctive, but composition was strongly spatially autocorrelated. Ecological similarity and species similarity of community pairs were strongly related, but replacement by ecologically similar species was common among drainage–physiography combinations. Landscape variables explained significant proportions of variance in community composition within selected subdrainages, but proportions were less than at the statewide scale, and the explanatory power of individual variables varied considerably among subdrainages. Community variation within subdrainages appeared to be much more closely related to environmental variation than to replacement among ecologically similar species. Our results suggest that taxonomic and ecological characterizations of community composition are complementary; both are useful in a conservation context. Landscape features such as drainage, physiography, and water body size generally may provide a basis for assessing aquatic community diversity, especially in regions where the biota is poorly known. Systematic conservation of community types would be a major advance relative to most current conservation programs, which typically focus narrowly on populations of imperiled species. More effective conservation of aquatic biodiversity will require new approaches that recognize the value of both species and assemblages, and that emphasize protection of key landscape-scale processes.

Ecological Applications↗

A global review of freshwater crayfish temperature tolerance, preference, and optimal growth

Conservation efforts, environmental planning, and management must account for ongoing ecosystem alteration due to a changing climate, introduced species, and shifting land use. This type of management can be facilitated by an understanding of the thermal ecology of aquatic organisms. However, information on thermal ecology for entire taxonomic groups is rarely compiled or summarized, and reviews of the science can facilitate its advancement. Crayfish are one of the most globally threatened taxa, and ongoing declines and extirpation could have serious consequences on aquatic ecosystem function due to their significant biomass and ecosystem roles. Our goal was to review the literature on thermal ecology for freshwater crayfish worldwide, with emphasis on studies that estimated temperature tolerance, temperature preference, or optimal growth. We also explored relationships between temperature metrics and species distributions. We located 56 studies containing information for at least one of those three metrics, which covered approximately 6 % of extant crayfish species worldwide. Information on one or more metrics existed for all 3 genera of Astacidae, 4 of the 12 genera of Cambaridae, and 3 of the 15 genera of Parastacidae. Investigations employed numerous methodological approaches for estimating these parameters, which restricts comparisons among and within species. The only statistically significant relationship we observed between a temperature metric and species range was a negative linear relationship between absolute latitude and optimal growth temperature. We recommend expansion of studies examining the thermal ecology of freshwater crayfish and identify and discuss methodological approaches that can improve standardization and comparability among studies.

Reviews in Fish Biology and Fisheries↗

Assessment of metal and trace element contamination in water, sediment, plants, macroinvertebrates, and fish in Tavasci Marsh, Tuzigoot National Monument, Arizona

Tavasci Marsh is a large freshwater marsh within the Tuzigoot National Monument in central Arizona. It is the largest freshwater marsh in Arizona that is unconnected to the Colorado River and is designated as an Important Bird Area by the Audubon Society. The marsh has been altered significantly by previous land use and the monument’s managers are evaluating the restoration of the marsh. In light of historical mining activities located near the marsh from the first half of the 20th century, evaluations of water, sediment, plant, and aquatic biota in the marsh were conducted. The evaluations were focused on nine metals and trace elements commonly associated with mining and other anthropogenic activities (As, Cd, Cr, Cu, Hg, Ni, Pb, Se, and Zn) together with isotopic analyses to understand the presence, sources and timing of water and sediment contaminants to the marsh and the occurrence in aquatic plants, dragonfly larvae, and fish. Results of water analyses indicate that there were two distinct sources of water contributing to the marsh during the study: one from older high elevation recharge entering the marsh at Shea Spring (as well as a number of unnamed seeps and springs on the northeastern edge of the marsh) and the other from younger low elevation recharge or from Pecks Lake. Water concentrations for arsenic exceeded the U.S. Environmental Protection Agency primary drinking water standard of 10 μg/L at all sampling sites. Surface waters at Tavasci Marsh may contain conditions favorable for methylmercury production. All surficial and core sediment samples exceeded or were within sample concentration variability of at least one threshold sediment quality guideline for As, Cd, Cr, Cu, Hg, Ni, Pb, and Zn. Several sediment sites were also above or were within sample concentration variability of severe or probable effect sediment quality guidelines for As, Cd, and Cu. Three sediment cores collected in the marsh have greater metal and trace element concentrations at depth for Bi, Cd, Cu, Hg, In, Pb, Sb, Sn, Te, and Zn. Radioisotope dating indicates that the elevated metal and trace element concentrations are associated with sediments deposited before 1963. Arsenic concentration was greater in cattail roots compared with surrounding sediment at Tavasci Marsh. Concentrations of As, Ni, and Se from yellow bullhead catfish (Ameiurus natalis) in Tavasci Marsh exceeded the 75th percentile of several other regional studies. Mercury concentration in dragonfly larvae and fish from Tavasci Marsh were similar to or greater than in Tavasci Marsh sediment. Future work includes a biologic risk assessment utilizing the data collected in this study to provide the monument management with additional information for their restoration plan.

Arizona↗

Biological effects of dietary T-2 toxin on rainbow trout, Salmo gairdneri

A 16-wk feeding study was conducted to evaluate the chronic toxicity of graded levels (0, 1.0, 2.5.5, 10 and 15 mg/kg of chemically pure dietary T-2 toxin (4,15-diacetoxy-8-(3-methylbutyryloxy)-12,13-epoxy-Δ 9 -tricothecen-3-ol) in 1-g rainbow trout, Salmo gairdneri , held in 9°C single-passage well water. Levels of T-2 toxin > 2.5 mg/kg depressed growth, efficiency of feed use, hematocrit, blood hemoglobin concentration and feed acceptance, and caused a transitory edema in a dose-dependent manner. Growth of trout fed a semipurified diet containing the toxin was described by the function: Y = 0.265 + 142.075 e (0.029 X 1 − 1.554x 2 3.7 ), where Y = gain as percentage starting weight per wk; X 1 is time in wk and 0 ⩽ X 1 ⩽16; and X 2 is T-2 content of diet in mg/kgand 0⩽ X 2 ⩽15. Exposure of fish to T-2 toxin did not affect activity of intestinal lumen chymoirypsin or trypsin, nitrogen digestibility or metabolizabte energy. Feeding of 15 mg/kg T-2 toxin to adult trout caused hemorrhaging in the intestines and regurgitation of subsequently intubated feed regardless of T-2 loxin content.

Aquatic Toxicology↗

Hydrographic and benthic mapping—St. Croix National Scenic Riverway—Osceola landing

High-resolution topographic and bathymetric mapping can assist in the analysis of river habitat. The National Park Service has been planning to relocate a boat ramp along the St. Croix River in Minnesota, across the river from the town of Osceola, Wisconsin, to improve visitor safety, improve operations for commercial use, enhance the overall visitor experience, and eliminate deferred maintenance at the landing. This landing grants access to the St. Croix River, which is a part of the National Park Service St. Croix National Scenic Riverway. Hydrographic and topographic surveys were needed to determine where the new location should be. The objective for these surveys was to provide baseline information in order to assess the direct effects of the landing relocation on physical habitat in areas adjacent to Osceola, Wisconsin. The study area for these surveys was about 18.5 hectares and located directly off the existing landing. Although the existing boat launch is referred to as the Osceola landing, it is located on the Minnesota side of the river and is the busiest National Park Service landing on the St. Croix River (National Park Service St. Croix National Scenic Riverway, 2020). This report documents methods and results of aquatic benthic mapping in a small area of the St. Croix River. The hydroacoustic and topographic surveys were collected from October 16–17, 2019. The hydrographic surveys consisted of multibeam and sidescan sound navigation and ranging (sonars). The topographic shoreline survey consisted of light detection and ranging (lidar) captured by boat adjacent to riverbanks. Additionally, an acoustic Doppler current profiler was used to measure flow velocities. The water level was higher than normal, and therefore had faster flow during the hydroacoustic surveys. Multibeam, lidar, and sidescan surveys occurred the first day, and the velocity mapping and ground truthing was conducted the second day. Multibeam and lidar provided derivative datasets that included bathymetry and a topobathy with a spatial resolution of 1 foot. From these data, additional data could be measured including slope and terrain ruggedness. Sidescan (acoustic reflectance measures) provided imagery that was used to help with interpretation of the river bottom. Outcomes from these combined datasets were substrate and bedform maps. Much of the area was covered in sand ripples or small dunes. A small area running adjacent to the deeper valley or cut down the river consisted of harder substrates, such as cobble and gravel. Large woody debris piles were found throughout the study area. Multiple stationary moving-bed tests were completed, and no corrections were recommended for the conditions occurring during survey. Mussel presence was noted in some of the underwater videos. The physical parameters of depth, flow, bedforms, and substrate derived from the datasets provided baseline measures for a benthic habitat map. Further analysis of benthic habitat might be possible with additional biological and chemical data.

Wisconsin↗

Habitat selection, movement patterns, and hazards encountered by northern leopard frogs (Lithobates pipiens) in an agricultural landscape

Telemetry data for 59 Northern Leopard Frogs (Lithobates pipiens) breeding in ponds in Houston and Winona Counties, MN; 2001-2002. Agricultural intensification is causing declines in many wildlife species, including Northern Leopard Frogs (Lithobates pipiens). Specific information about frog movements, habitat selection, and sources of mortality can be used to inform conservation-focused land management and acquisition. We studied Northern Leopard Frogs in southeastern Minnesota, part of the Driftless Area ecoregion, characterized by hills and valleys and a mix of agriculture, forests, small towns and farmsteads. In this area, small farm ponds, originally built to control soil erosion are used by the species for breeding and wintering in addition to riparian wetlands. But, this agricultural landscape may be hazardous for frogs moving between breeding, feeding, and wintering habitats. We surgically implanted transmitters into the peritoneal cavity of 59 Northern Leopard Frogs and tracked them from May to October 2001-2002. The total distance traveled by radio-tagged frogs ranged from 12 to 3316 m, the 95% home range averaged 5.3 ± 1.2 (SE) ha, and the 50% core area averaged 1.05 ± 0.3 (SE) ha. As expected, Northern Leopard Frogs selected wetlands over all other land cover classes and row crops were generally avoided at all levels of selection. Only a few tracked frogs were successful at dispersing (n = 6). Most frogs attempting to disperse (n =31) ended up missing (n = 14), died due to mowing (n = 8), or were recorded as transmitter failure (n = 2) or unknown mortalities (n = 1). For the conservation of Northern Leopard Frogs in this agricultural setting, we must consider both the aquatic and the terrestrial needs of this species. Conservation agencies that restore, manage, and acquire wetlands should consider the hazards posed by land uses adjacent to frog breeding and wintering sites and plan for movement corridors between these locations. For example, grasslands that are mowed or hayed between April and October in the north central U.S. and are adjacent to wetlands, pose a direct threat to frogs because these cultivated grasslands are primary locations for summer occupancy. When conservation land managers are selecting sites for acquisition or restoration they should avoid investments that will situate the wetland adjacent to heavily travelled roads and agricultural lands likely to be mowed or hayed. Increasing habitat amount and quality at amphibian breeding, feeding and wintering sites should reduce the energy required and hazards associated with moving long distances. Large, diverse wetlands probably provide all of the requirements needed by Northern Leopard Frogs for survival including food, shelter, breeding and overwintering areas.

Minnesota↗

Structure of marine predator and prey communities along environmental gradients in a glaciated fjord

Spatial patterns of marine predator communities are influenced to varying degrees by prey distribution and environmental gradients. We examined physical and biological attributes of an estuarine fjord with strong glacier influence to determine the factors that most influence the structure of predator and prey communities. Our results suggest that some species, such as walleye pollock ( Theragra chalcogramma ), black-legged kittiwake ( Rissa tridactyla ), and glaucous-winged gull ( Larus glaucescens ), were widely distributed across environmental gradients, indicating less specialization, whereas species such as capelin ( Mallotus villosus ), harbor seal ( Phoca vitulina ), and Kittlitz's murrelet ( Brachyramphus brevirostris ) appeared to have more specialized habitat requirements related to glacial influence. We found that upper trophic level communities were well correlated with their mid trophic level prey community, but strong physical gradients in photic depth, temperature, and nutrients played an important role in community structure as well. Mid-trophic level forage fish communities were correlated with the physical gradients more closely than upper trophic levels were, and they showed strong affinity to tidewater glaciers. Silica was closely correlated with the distribution of fish communities, the mechanisms of which deserve further study.

Alaska↗

Pacific salmonines in the Great Lakes Basin

Pacific salmon (genus Oncorhynchus) are a valuable resource, both within their native range in the North Pacific rim and in the Great Lakes basin. Understanding their value from a biological and economic perspective in the Great Lakes, however, requires an understanding of changes in the ecosystem and of management actions that have been taken to promote system stability, integrity, and sustainable fisheries. Pacific salmonine introductions to the Great Lakes are comprised mainly of Chinook salmon, coho salmon, and steelhead and have accounted for 421, 177, and 247 million fish, respectively, stocked during 1966-2007. Stocking of Pacific salmonines has been effective in substantially reducing exotic prey fish abundances in several of the Great Lakes (e.g., lakes Michigan, Huron, and Ontario). The goal of our evaluation was to highlight differences in management strategies and perspectives across the basin, and to evaluate policies for Pacific salmonine management in the Great Lakes. Currently, a potential conflict exists between Pacific salmonine management and native fish rehabilitation goals because of the desire to sustain recreational fisheries and to develop self-sustaining populations of stocked Pacific salmonines in the Great Lakes. We provide evidence that suggests Pacific salmonines have not only become naturalized to the food webs of the Great Lakes, but that their populations (specifically Chinook salmon) may be fluctuating in concert with specific prey (i.e., alewives) whose populations are changing relative to environmental conditions and ecosystem disturbances. Remaining questions, however, are whether or not “natural” fluctuations in predator and prey provide enough “stability” in the Great Lakes food webs, and even more importantly, would a choice by managers to attempt to reduce the severity of predator-prey oscillations be antagonistic to native fish restoration efforts. We argue that, on each of the Great Lakes, managers are pursuing appropriate goals, managing the aquatic resources of the lakes for the greatest public good, given the variability in conditions and likelihood for success specific to each lake.

Book chapter↗

Integrating side-scan sonar and acoustic telemetry to estimate the annual spawning run size of Atlantic sturgeon in the Hudson River

There is considerable interest in evaluating the status and trends of sturgeon populations, yet many traditional approaches to estimating the abundance of fishes are intractable due to their biology and rarity. Side-scan sonar has recently emerged as an effective tool for censusing sturgeon in rivers, yet challenges remain for censusing open populations that may visit specific habitats over periods of time (e.g., spawning runs). We use a hierarchical model to integrate side-scan sonar with acoustic telemetry, to estimate the proportion of a spawning run fitted with acoustic tags (12%; 95% CRI = 8-16%) and extrapolate to the total run size in 2014. Our investigation represents a novel approach to generating run size estimates in a large river and provides the first estimate of Atlantic sturgeon spawning run size for the Hudson River (N ̂ = 466; 95% CRI = 310-745) since the fishery moratorium in the 1990’s. Our estimate suggests that the Hudson River holds one of the largest contemporary populations of Atlantic sturgeon, but also indicates that it remains sharply depleted relative to virgin conditions.

Hudson River↗

Taxonomic evaluation of the three “type” specimens of the fringe-footed shrew, Sorex fimbripes Bachman, 1837 (Mammalia: Soricidae) and recommended nomenclatural status of the name

John Bachman (1837:391) described the “fringe-footed shrew,” Sorex fimbripes Bachman, 1837, in his landmark monograph on the North American Soricidae (Mammalia: Eulipotyphla), in which he recognized 13 uniquely New World species. Characters he attributed to S. fimbripes resulted in its being interpreted as a tiny, semi-aquatic species and contributed to the complexity of its subsequent taxonomic history. The status and location of the holotype, which should aid in resolving questions about the nature of S. fimbripes, instead have added to the confusion. Originally studied by Bachman in South Carolina, the holotype was later thought to have been identified in the Academy of Natural Sciences of Philadelphia (ANSP), and it is currently considered to be in the National Museum of Natural History (USNM), Washington, D.C. To clarify the identity of the species and its holotype, I compared descriptions of the holotype and the ANSP type with the USNM specimen and with eight species of eastern North American shrews. I conclude that the three accounts of S. fimbripes refer to three different specimens and that the holotype was probably destroyed during the American Civil War. Bachman's S. fimbripes was most likely equivalent to S. cinereus Kerr, 1792, S. fontinalis Hollister, 1911, or S. fumeus Miller, 1895, but without the holotype, its identity cannot be determined definitively, and the name is a nomen dubium.

Proceedings of the Biological Society of Washingto↗