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At least 919 records · Page 51Linked to original sources

Eyes on the herd: Quantifying ungulate density from satellite, unmanned aerial systems, and GPScollar data

Novel approaches to quantifying density and distributions could help biologists adaptively manage wildlife populations, particularly if methods are accurate, consistent, cost-effective, rapid, and sensitive to change. Such approaches may also improve research on interactions between density and processes of interest, such as disease transmission across multiple populations. We assess how satellite imagery, unmanned aerial system (UAS) imagery, and Global Positioning System (GPS) collar data vary in characterizing elk density, distribution, and count patterns across times with and without supplemental feeding at the National Elk Refuge (NER) in the US state of Wyoming. We also present the first comparison of satellite imagery data with traditional counts for ungulates in a temperate system. We further evaluate seven different aggregation metrics to identify the most consistent and sensitive metrics for comparing density and distribution across time and populations. All three data sources detected higher densities and aggregation locations of elk during supplemental feeding than non-feeding at the NER. Kernel density estimates (KDEs), KDE polygon areas, and the first quantile of interelk distances detected differences with the highest sensitivity and were most highly correlated across data sources. Both UAS and satellite imagery provide snapshots of density and distribution patterns of most animals in the area at lower cost than GPS collars. While satellite-based counts were lower than traditional counts, aggregation metrics matched those from UAS and GPS data sources when animals appeared in high contrast to the landscape, including brown elk against new snow in open areas. UAS counts of elk were similar to traditional ground-based counts on feed grounds and are the best data source for assessing changes in small spatial extents. Satellite, UAS, or GPS data can provide appropriate data for assessing density and changes in density from adaptive management actions. For the NER, where high elk densities are beneath controlled airspace, GPS collar data will be most useful for evaluating how management actions, including changes in the dates of supplemental feeding, influence elk density and aggregation across large spatial extents. Using consistent and sensitive measures of density may improve research on the drivers and effects of density within and across a wide range of species.

Wyoming↗

Fire risk in revegetated bunchgrass communities Infested with Bromus tectorum

In rangeland ecosystems, invasive annual grass replacement of native perennials is associated with higher fire risk. Large bunchgrasses are often seeded to reduce cover of annuals such as Bromus tectorum L. (cheatgrass), but there is limited information about how revegetation reduces fire risk over the long-term. For this research note, we conducted a pilot study to assess how community composition influences fire risk at 3 sites in Columbia National Wildlife Refuge in Washington, USA that were revegetated with large bunchgrasses 8 to 18 years prior to the study. At each revegetated site, five replicates with 10 plots each (plot size 10 × 10 m) were established. Fire risk was determined as the probability that a plot would completely burn following ignition at a randomly located point in each plot (i.e., if 8 of 10 plots burned fire risk was 80%). Pre-ignition, cover of bunchgrasses, cheatgrass, forbs, and surface cover characteristics were determined for each plot. Bunchgrasses successfully established to varying degrees among sites, but fire risk was still relatively high (around 73%) and did not significantly differ among sites cover. All sites had similar surface cover characteristics such as high total fuels cover (>80% at all sites) and low unvegetated gap cover (soil and soil cryptogams, <17%). Our pilot study suggests that even though revegetation may increase bunchgrass and decrease cheatgrass, fire risk remains high likely due to other surface cover characteristics. Future studies should aim to have larger variation in cover, grazing intensity, and weather conditions.

Washington↗

The conservation genetics juggling act: Integrating genetics and ecology, science and policy

The field of conservation genetics, when properly implemented, is a constant juggling act integrating molecular genetics, ecology, and demography with applied aspects concerning managing declining species or implementing conservation laws and policies. This young field has grown substantially since the 1980&rsquo;s following development of the polymerase chain reaction and now into the genomics era. Our lab has &ldquo;grown up&rdquo; with the field, having worked on these issues for over three decades. Our multi-disciplinary approach entails understanding the behavior and ecology of species as well as the underlying processes that contribute to genetic viability. Taking this holistic approach provides a comprehensive understanding of factors that influence species persistence and evolutionary potential while considering annual challenges that occur throughout their life cycle. As a federal lab, we are often addressing the needs of the U.S. Fish and Wildlife Service in their efforts to list, de-list or recover species. Nevertheless, there remains an overall communication gap between research geneticists and biologists who are charged with implementing their results. Therefore, we outline the need for a National Center for Small Population Biology to ameliorate this problem and provide organizations charged with making status decisions firmer ground from which to make their critical decisions.

Evolutionary Applications↗

Geostatistical analysis of allele presence patterns among American black bears in eastern North Carolina

Highways are one of the leading causes of wildlife habitat fragmentation and may particularly affect wide-ranging species, such as American black bears (Ursus americanus). We initiated a research project in 2000 to determine potential effects of a 4-lane highway on black bear ecology in Washington County, North Carolina. The research design included a treatment area (highway construction) and a control area and a pre- and post-construction phase. We used data from the pre-construction phase to determine whether we could detect scale dependency or directionality among allele occurrence patterns using geostatistics. Detection of such patterns could provide a powerful tool to measure the effects of landscape fragmentation on gene flow. We sampled DNA from roots of black bear hair at 70 hair-sampling sites on each study area for 7 weeks during fall of 2000. We used microsatellite analysis based on 10 loci to determine unique multi-locus genotypes. We examined all alleles sampled at ???25 sites on each study area and mapped their presence or absence at each hair-sample site. We calculated semivariograms, which measure the strength of statistical correlation as a function of distance, and adjusted them for anisotropy to determine the maximum direction of spatial continuity. We then calculated the mean direction of spatial continuity for all examined alleles. The mean direction of allele frequency variation was 118.3?? (SE = 8.5) on the treatment area and 172.3?? (SE = 6.0) on the control area. Rayleigh's tests showed that these directions differed from random distributions (P = 0.028 and P < 0.001, respectively), indicating consistent directional patterns for the alleles we examined in each area. Despite the small spatial scale of our study (approximately 11,000 ha for each study area), we observed distinct and consistent patterns of allele occurrence, suggesting different directions of gene flow between the study areas. These directions seemed to coincide with the primary orientation of the best habitat areas. Furthermore, the patterns we observed suggest directions of potential source populations beyond the 2 study areas. Indeed, nearby areas classified as core black bear habitat exist in the directions indicated by our analysis. Geostatistical analysis of allele occurrence patterns may provide a useful technique to identify potential barriers to gene flow among bear populations.

Ursus↗

USGS Science Serves Public Health

Human health so often depends on the health of the environment and wildlife around us. The presence of naturally occurring or human environmental contaminants and the emergence of diseases transferred between animals and humans are growing concerns worldwide. The USGS is a source of natural science information vital for understanding the quantity and quality of our earth and living resources. This information improves our understanding not only of how human activities affect environmental and ecological health, but also of how the quality of our environment and wildlife in turn affects human health. USGS is taking a leadership role in providing the natural science information needed by health researchers, policy makers, and the public to safeguard public health

Fact Sheet↗

Optimization of salt marsh management at the Rachel Carson National Wildlife Refuge, Maine, through use of structured decision making

Structured decision making is a systematic, transparent process for improving the quality of complex decisions by identifying measurable management objectives and feasible management actions; predicting the potential consequences of management actions relative to the stated objectives; and selecting a course of action that maximizes the total benefit achieved and balances tradeoffs among objectives. The U.S. Geological Survey, in cooperation with the U.S. Fish and Wildlife Service, applied an existing, regional framework for structured decision making to develop an example of a prototype tool for optimizing tidal marsh management decisions for selected marsh management units at the Rachel Carson National Wildlife Refuge in Maine. The goal was to create a prototype that could be available for future implementation. Refuge biologists, refuge managers, and research scientists identified multiple potential management actions to improve the ecological integrity of seven marsh management units within the refuge and estimated the outcomes of each action in terms of regional performance metrics associated with each management objective. Value functions previously developed at the regional level were used to transform metric scores to a common utility scale, and utilities were summed to produce a single score representing the total management benefit that could be accrued from each potential management action. Constrained optimization was used to identify the set of management actions, one per marsh management unit, that could maximize total management benefits at different cost constraints at the refuge scale. Management costs were estimated using limited available information, and estimated costs of individual management actions reflected relative differences among actions rather than actual expected expenditures. Results from this prototype showed how, for the objectives, actions, and estimated outcomes used for this example, total management benefits may increase consistently up to a certain estimated cost, and may continue to increase, at a lower rate, with further expenditures. Potential management actions in optimal portfolios at moderate total estimated costs included breaching or removing dikes, roads, or embankments; planting Spartina alterniflora (smooth cordgrass); and digging runnels, or shallow creeks, on the marsh platform to improve surface-water drainage. Potential management actions in optimal portfolios at high estimated costs (for example, up to $550,000) included breaching embankments to restore tidal exchange followed by planting salt marsh vegetation. The potential management benefits were derived from predicted increases in the numbers of tidal marsh obligate birds and spiders (as an indicator of trophic health), and expected improvement in the capacity of marsh elevation to keep pace with sea-level rise and reduced duration of marsh-surface inundation. The prototype presented here does not resolve current management decisions; rather, it provides a framework for decision making at the Rachel Carson National Wildlife Refuge that can be updated for implementation as new data and information become available. Insights from this process may also be useful to inform future habitat management planning at the refuges.

Maine↗

A critical time for mercury science to inform global policy

Mercury is a global pollutant released into the biosphere by varied human activities including coal combustion, mining, artisanal gold mining, cement production, and chemical production. Once released to air, land and water, the addition of carbon atoms to mercury by bacteria results in the production of methylmercury, the toxic form that bioaccumulates in aquatic and terrestrial food chains resulting in elevated exposure to humans and wildlife. Global recognition of the mercury contamination problem has resulted in the Minamata Convention on Mercury, which came into force in 2017. The treaty aims to protect human health and the environment from human-generated releases of mercury curtailing its movement and transformations in the biosphere. Coincident with the treaty’s coming into force, the 13th International Conference of Mercury as a Global Pollutant (ICMGP-13) was held in Providence, Rhode Island USA. At ICMGP-13, cutting edge research was summarized and presented to address questions relating to global and regional sources and cycling of mercury, how that mercury is methylated, the effects of mercury exposure on humans and wildlife, and the science needed for successful implementation of the Minamata Convention. Human activities have the potential to enhance mercury methylation by remobilizing previously released mercury, and increasing methylation efficiency. This synthesis concluded that many of the most important factors influencing the fate and effects of mercury and its more toxic form, methylmercury, stem from environmental changes that are much broader in scope than mercury releases alone. Alterations of mercury cycling, methylmercury bioavailability and trophic transfer due to climate and land use changes remain critical uncertainties in effective implementation of the Minamata Convention. In the face of these uncertainties, important policy and management actions are needed over the short-term to support the control of mercury releases to land, water and air. These include adequate monitoring and communication on risk from exposure to various forms of inorganic mercury as well as methylmercury from fish and rice consumption. Successful management of global and local mercury pollution will require integration of mercury research and policy in a changing world.

Environmental Science & Technology↗

Understanding the Avian-Impact Offset Method—A tutorial

Biodiversity offsetting, or compensatory mitigation, is increasingly being used in temperate grassland and wetland ecosystems to compensate for unavoidable environmental damage from anthropogenic disturbances such as energy development and road construction. Energy-extraction and -generation facilities continue to proliferate across the natural landscapes of the United States, yet mitigation tools to ameliorate the negative behavioral effects on wildlife from these types of facilities are rarely implemented. Scientists from the U.S. Geological Survey conducted a 10-year before-after-control-impact (commonly referred to as BACI) study that evaluated the displacement effects of wind facilities on breeding grassland birds. The study determined behavioral avoidance for 7 of 9 species. This research is notable because of its design, geographical scope, and duration, which allowed for the determination of immediate, short-term effects; delayed or sustained effects; and discrete distances at which effects occurred. In addition, the U.S. Fish and Wildlife Service and Ducks Unlimited conducted a 3-year concurrent-year paired-reference study to determine behavioral avoidance for five species of dabbling ducks. By quantifying displacement rate from these two studies, U.S. Geological Survey and U.S. Fish and Wildlife Service scientists developed the Avian-Impact Offset Method (AIOM) to quantify and compensate for loss in value of breeding habitat. The AIOM converts the biological value (that is, number of bird pairs) lost by way of avoidance and estimates the site-specific number of hectares of grasslands and number of wetlands needed to compensate for displaced pairs of grassland birds and waterfowl. By converting biological value to traditional units of measure in which land is described and purchased or sold, the AIOM lends itself readily to the delivery of offsetting measures such as easement protections and restoration projects. The AIOM tool is applicable to wind, solar, oil, gas, and transportation infrastructure. This tutorial was designed to increase awareness of the AIOM and to promote its proper application. The tutorial is divided into four sections, each of which explains a discrete topic concerning aspects of behavioral displacement. The first section provides geographical and biological context, and the second section describes the field and statistical methods and results. The third section provides step-by-step instructions for applying the AIOM to several scenarios involving grassland birds or waterfowl at wind or oil facilities. The fourth section describes decision-support tools created to implement the AIOM. The appendices provide the actual field protocols constituting the methods for the research, provide detailed results by species and wind facility for that research, and provide detailed instructions for downloading and applying the decision-support tools.

Open-File Report↗

Management implications of habitat selection by whooping cranes (Grus americana) on the Texas coast

Effective habitat management for rare and endangered species requires a thorough understanding of their specific habitat requirements. Although machine learning models have been increasingly used in the analyses of habitat use by wildlife, the primary focus of these models has been on generating spatial predictions. In this study, we used machine learning models in combination with simulated management actions to guide planning and inform managers. We used data from 61 whooping cranes ( Grus americana ) tagged with GPS telemetry collars between 2009 and 2018 near Aransas National Wildlife Refuge in coastal Texas. We included variables based on topography, land use classification, vegetation height, plant phenology, drought, storm surge events, and both wild and prescribed fires. We then built models at multiple scales: population level, home range level, and roosting and daytime within home range level. We simulated responses to the two primary management actions used to enhance whooping crane habitat on Aransas National Wildlife Refuge: prescribed fire and removal of woody vegetation. At the population and home range scales, land use classification variables had the highest importance values, whereas the combined elevation and bathymetry layer was the most important predictor at both roosting and daytime within home range scales. Our findings revealed that the effects of fire, although generally modest, varied spatially. Areas dominated by estuarine wetlands exhibited higher predicted use within the first months after a fire, whereas those dominated by palustrine wetlands were more likely to be avoided in the immediate postfire years. Our simulation of vegetation removal identified the areas on Aransas National Wildlife Refuge where whooping cranes were predicted to benefit the most if vegetation were removed. These techniques can be used by other researchers wanting to examine and predict the effects of potential management actions on target species habitat.

Texas↗

U.S. Geological Survey — Department of the Interior Region 11, Alaska—2019 annual science report

In keeping with our national mission, the USGS in Alaska provides timely and objective scientific information to help the Nation address issues and solve problems in five major topical areas (listed alphabetically): Energy and Minerals; Geospatial Mapping; Natural Hazards; Water Quality, Streamflow, and Ice Dynamics; and Wildlife, Fish, and Habitat. The USGS in Alaska engages about 400 scientists and support staff working in three major science centers, Cooperative Research Units, and USGS centers outside Alaska, with a combined annual science budget of about $60 million. In just the last 5 years, the USGS in Alaska has produced scientific benefits resulting from more than 1,000 publications and about 250 technical reports. Publications relevant to Alaska can be conveniently searched by keyword through the USGS Publications Warehouse at https://pubs.er.usgs.gov/search?q=Alaska .

Alaska↗

Greater sage-grouse habitat of Nevada and northeastern California—Integrating space use, habitat selection, and survival indices to guide areas for habitat management

Executive Summary Greater sage-grouse populations ( Centrocercus urophasianus ; hereafter sage-grouse) are threatened by a suite of disturbances and anthropogenic factors that have contributed to a net loss of sagebrush-dominant shrub cover in recent decades. Declines in sage-grouse populations are largely linked to habitat loss across their range. A key component of conservation and land use planning efforts for sage-grouse involves the continued monitoring and modeling of habitat requirements and suitability across its range. The Bureau of Land Management (BLM) is addressing the management of sage-grouse habitats on BLM-authorized public lands throughout the western United States through a land use planning amendment and associated environmental impact statement (86 FR 66331). More than 25 percent of the range-wide distribution of sage-grouse is within Nevada and northeastern California, and information on sage-grouse distribution and habitat requirements is important to guide appropriate management decisions. Therefore, the BLM has identified the need for updated spatially explicit information on sage-grouse habitat in Nevada and northeastern California to guide the land use planning amendment and associated management decisions. To address this need, researchers with the U.S. Geological Survey, in close cooperation with multiple State and Federal resource agency partners, including BLM, Nevada Department of Wildlife (NDOW) and California Department of Fish and Wildlife (CDFW), sought to map sage-grouse distribution and produce example habitat designations in these states. Herein, we report results of our primary study objective, which was to map sage-grouse habitat and create example habitat management areas, based on more than a decade of location and survival data collected from marked sage-grouse across the study region coupled with lek count survey data managed by the NDOW and the CDFW. We expanded on previously developed methodology to incorporate information on habitat selection and survival during reproductive life stages and specific seasons with updated sage-grouse location and known fate datasets, while also including brood-rearing areas that are understood to be threatened and important for population persistence. We combined predictive habitat map surfaces for each life stage and season with updated information on current occupancy patterns to classify habitat based on its suitability and probability of occupancy. We carried out additional steps to delineate specific example habitat management areas, specifically (1) incorporated corridors connecting key nesting and brood-rearing habitat, (2) corrected outputs for pre-wildfire habitat conditions within areas burned in the last 16 years, and (3) masked out areas of anthropogenic development. Our methodological example of deriving habitat management areas was intended to help inform decisions by BLM and other land managers regarding conservation and management of sage-grouse. Associated data products in the form of habitat maps provide updated, detailed, and comprehensive information about the status of habitats and can be useful to partner agencies in their efforts to designate and rank habitats for this species of high conservation concern in Nevada and California, with full recognition that on-the-ground field data and local sources of information and expertise should be used in conjunction with inferences from these models.

California, Idaho, Nevada↗

An update on USGS studies of the Summitville Mine and its downstream environmental effects

The Summitville gold mine, located at ~3800 meters (11,500 ft) elevation in the San Juan Mountains of southwestern Colorado, was the focus of extensive public attention in 1992 and 1993 for environmental problems stemming from recent open-pit mining activities. Summitville catalyzed national debates about the environmental effects of modern mining activities, and became the focus of arguments for proposed revisions to the 1872 Mining Law governing mining activities on public lands. In early 1993, the State of Colorado, U.S. Environmental Protection Agency (EPA), U.S. Geological Survey (USGS), U.S. Fish and Wildlife Service (USFWS), Colorado State University, San Luis Valley agencies, downstream water users, private companies, and individuals began a multi-disciplinary research program to provide needed scientific information on Summitville's environmental problems and downstream environmental effects. Detailed results of this multi-agency effort were presented, along with legal and policy issues, at the Summitville Forum in January, 1995, at Colorado State University, Fort Collins, Colorado.

Open-File Report↗

Forestry best management practices relationships with aquatic and riparian fauna: A review

Forestry best management practices (BMPs) were developed to minimize water pollution from forestry operations by primarily addressing sediment and sediment transport, which is the leading source of pollution from silviculture. Implementation of water quality BMPs may also benefit riparian and aquatic wildlife, although wildlife benefits were not driving forces for BMP development. Therefore, we reviewed literature regarding potential contributions of sediment-reducing BMPs to conservation of riparian and aquatic wildlife, while realizing that BMPs also minimize thermal, nutrient, and chemical pollution. We reached five important conclusions: (1) a significant body of research confirms that forestry BMPs contribute to the protection of water quality and riparian forest structure; (2) data-specific relationships between forestry BMPs and reviewed species are limited; (3) forestry BMPs for forest road construction and maintenance, skid trails, stream crossings, and streamside management zones (SMZs) are important particularly for protection of water quality and aquatic species; (4) stream crossings should be carefully selected and installed to minimize sediment inputs and stream channel alterations; and (5) SMZs promote retention of older-age riparian habitat with benefits extending from water bodies to surrounding uplands. Overall, BMPs developed for protection of water quality should benefit a variety of riparian and aquatic species that are sensitive to changes in water quality or forest structure.

Forests↗

Direct effects of pesticides and other grassland management practices on the North American Monarch Butterfly (Danaus plexippus plexippus): A systematic review

This review synthesizes recent scientific literature on the effects of grassland management practices and pesticide applications on monarch butterflies ( Danaus plexippus ), focusing on studies published since the 2020 U.S. Fish and Wildlife Service Species Status Assessment (SSA). While the SSA highlighted habitat conservation efforts to enhance monarch populations, there has been limited empirical research on the direct effects of these management strategies on monarch survival and fitness. Following PRISMA guidelines, we conducted a systematic review to evaluate research published during and after the SSA (2019-2024) on the direct effects of common grassland management practices (mowing, grazing, burning, haying) and pesticides (herbicides, insecticides, fungicides) across monarch life stages. This search identified 29 studies that met our criteria. Of these, only two studies addressed the impacts of grassland management on monarchs, indicating a substantial and important information gap. The other 27 studies reviewed indicate significant risks of pesticide exposure to monarchs, particularly at immature life stages from lethal and sublethal effects. The effects of chronic insecticide exposure, particularly neonicotinoids, are especially critical to consider, as research shows insecticides can result in impairment of growth, development, and reproductive behaviors. Additionally, fungicides pose overlooked risks to monarchs due to their toxicity to non-target organisms. Notably, most studies evaluating pesticide effects were conducted in a laboratory setting and thus it is unclear how these results relate to fieldrealistic exposure, as well as exposure over multiple life history stages and potential synergistic effects of pesticides. Additional relevant information gaps remain, especially on studies of southern migratory paths critical for monarch population persistence. Data gaps at relevant scales for grassland management combined with growing evidence of pesticide persistence and subsequent threats to insects, highlight how more ecologically applicable metrics for monitoring environmental chemicals could help improve understanding of the effects of pesticides on monarchs and characterize contamination of milkweed and nectar resources.

Cooperator Science Series↗

Associations between Ornithodoros spp. ticks and Mojave Desert Tortoises (Gopherus agassizii) obtained from health assessment documents

Soft ticks in the genus Ornithodoros occur throughout the Mojave Desert in southern Nevada, southeastern California, and parts of southwestern Utah and northwestern Arizona, USA, and are frequently observed parasitizing Mojave desert tortoises ( Gopherus agassizii ). However, limited research exists examining the relationship between ticks and desert tortoises. Mojave desert tortoises are listed as threatened by the US Fish and Wildlife Service, and as such, their populations are monitored and individual tortoise health is routinely assessed. These health assessments document the presence and abundance of ticks present on tortoises, but detailed examination of the relationship between ticks and tortoise health has been lacking. This study analyzed the relationship between tick presence and desert tortoise health assessments as a function of season, location, age (adult vs. juvenile), foraging behavior, evidence of clinical signs of disease, body condition score, and sex. Our results indicate that more ticks were found on tortoises in the summer than in any other season. Ticks were observed more frequently on captive tortoises versus wild tortoises, and more ticks were likely to be present on adult tortoises than on juveniles. Ticks were also more likely to be observed on tortoises that lacked evidence of foraging and on tortoises with observed clinical signs of disease. These findings provide valuable insights into the biology of ticks in relation to tortoises that may be useful for management of both captive and free-living threatened tortoise populations where ticks are detected. Our study also may improve understanding of potential tick-borne disease dynamics in the Mojave desert tortoise habitat, including Borrelia sp. carried by Ornithodoros ticks, which cause tick-borne relapsing fever in people.

Journal of Wildlife Diseases↗

Macracanthorhynchus ingens from raccoons in Maryland

There have been very few published records of the occurrence of the acanthocephalan parasite Macracanthorhynchus ingens (von Linstow, 1897) in North America. Chandler (1942) and Moore (1946) reported 11 o 13 raccoons ( Procyon lotor ) trapped in Angelina County, Texas, infected with these intestinal parasites. From 1 to 90 worms occurred in these animals. Chandler and Melvin (1951), in a study of parasites collected from mammals in Pennsylvania, reported M. ingens common in its usual host, the raccoon, and also found immature worms believed to belong to this species in skunk, Mephitis nigra ; mink, Mustela vision ; fox, Urocyon cineraoargenteus ; and mole, Parascalops breweri . Van Cleave (1953), in a review of North American Acanthocephala, lists the definitive host as raccoon of the States of Texas and Pennsylvania. He further states: “It is thought that the apparent discontinuous distribution of M. ingens is due to incomplete records of its occurrence in intervening states.” Subsequently, Goldberg (1954) reported 5 specimens of M. ingens in one of 14 skunks from Beltsville, Md. This parasite appears to be fairly common in raccoons from Maryland, as indicated by material from two stations in the State. Of 44 intestinal tracts collected during the winter months, 1943-1946, at the Patuxent Research Refuge, laurel, Maryland, 22 (50%) contained M. ingens in their intestines (Ediger, 1950). Numbers of these parasites per host varied from 1 to 125. Since then, additional raccoons from the Patuxent Research refuge have been found infected with M. ingens . The parasite has also been noted in raccoons collected from the Blackwater National Wildlife refuge and surrounding areas, near Cambridge on the eastern shore of Maryland. One raccoon obtained in May, 1950, contained five specimens of M. ingens . In a series of raccoons obtained by V.T. Harris, during the winter months 1950-1951, 32 (37%) of 86 raccoons examined were infected with M. ingens . Intensity varied from 1 to 44 worms per raccoon.

Maryland↗

Developing accurate survey methods for estimating population sizes and trends of the critically endangered Nihoa Millerbird and Nihoa Finch.

This report describes the results of a comparative study of bird survey methods undertaken for the purpose of improving assessments of the conservation status for the two endemic passerines on the Island of Nihoa&mdash;Nihoa Millerbird (Sylviidae: Acrocephalus familiaris kingi ) and Nihoa Finch (Fringilidae: Telespiza ultima ; also referred herein as millerbird and finch)&mdash;both listed as endangered under the Federal Endangered Species Act (ESA) and Hawai`i Revised Statutes 195D. The current survey protocol, implemented since 1967, has produced a highly variable range of counts for both the millerbird and finch, making difficult assessments of population size and trend. This report details the analyses of bird survey data collected in 2010 and 2011 in which three survey methods were compared―strip-transect, line-transect, and point-transect sampling―and provides recommendations for improved survey methods and protocols. Funding for this research was provided through a Science Support Partnership grant sponsored jointly by the U.S. Geological Survey (USGS) and the U.S. Fish and Wildlife Service (USFWS). Point-transect surveys indicated that millerbirds were more abundant than shown by the striptransect method, and were estimated at 802 birds in 2010 (95%CI = 652 &ndash; 964) and 704 birds in 2011 (95%CI = 579 &ndash; 837). Point-transect surveys yielded population estimates with improved precision which will permit trends to be detected in shorter time periods and with greater statistical power than is available from strip-transect survey methods. Mean finch population estimates and associated uncertainty were not markedly different among the three survey methods, but the performance of models used to estimate density and population size are expected to improve as the data from additional surveys are incorporated. Using the pointtransect survey, the mean finch population size was estimated at 2,917 birds in 2010 (95%CI = 2,037 &ndash; 3,965) and 2,461 birds in 2011 (95%CI = 1,682 &ndash; 3,348). Preliminary testing of the line-transect method in 2011 showed that it would not generate sufficient detections to effectively model bird density, and consequently, relatively precise population size estimates. Both species were fairly evenly distributed across Nihoa and appear to occur in all or nearly all available habitat. The time expended and area traversed by observers was similar among survey methods; however, point-transect surveys do not require that observers walk a straight transect line, thereby allowing them to avoid culturally or biologically sensitive areas and minimize the adverse effects of recurrent travel to any particular area. In general, pointtransect surveys detect more birds than strip-survey methods, thereby improving precision and resulting population size and trend estimation. The method is also better suited for the steep and uneven terrain of Nihoa

Hawaii↗