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The SCEC/USGS community stress drop validation study using the 2019 Ridgecrest earthquake sequence

We introduce a community stress drop validation study using the 2019 Ridgecrest, California, earthquake sequence, in which researchers are invited to use a common dataset to independently estimate comparable measurements using a variety of methods. Stress drop is the change in average shear stress on a fault during earthquake rupture, and as such is a key parameter in many ground motion, rupture simulation, and source physics problems in earthquake science. Spectral stress drop is commonly estimated by fitting the shape of the radiated energy spectrum, yet estimates for an individual earthquake made by different studies can vary hugely. In this community study, sponsored jointly by the U. S. Geological Survey and Southern/Statewide California Earthquake Center, we seek to understand the sources of variability and uncertainty in earthquake stress drop through quantitative comparison of submitted stress drops. The publicly available dataset consists of nearly 13,000 earthquakes of M1 to 7 from two weeks of the 2019 Ridgecrest sequence recorded on stations within 1-degree. As a community study, findings are shared through workshops and meetings and all are invited to join at any time, at any interest level.

Seismica

Current status of the community sensor model standard for the generation of planetary digital terrain models

The creation of accurate elevation models (topography) from stereo images are critical for a large variety of geospatial activities, including the production of digital orthomosaics, change detection, landing site analysis, geologic mapping, rover traverse planning, and spectral analysis. The United Stated Geological Survey, Astrogeology Science Center, continues to transition the supported planetary sensor models to the Community Sensor Model (CSM) standard. This paper describes the current state of use for this photogrammetric standard, supported sensor model types, and qualitatively compares derived topography between SOCET SET and SOCET GXP ( ® BAE Systems) using HiRISE stereo images of Mars. Our transition to the CSM standard will ensure an uninterrupted capability to make these valuable products for Mars and many other extraterrestrial planets and moons.

Remote Sensing

Influence of new residential construction varying in housing density on bird species, human tolerance guilds, and communities

Human population growth and changing settlement patterns fuel the development of urban fringe lands worldwide, with implications for biodiversity. We conducted a 12-year study of birds in the fast-developing urban fringe lands of the central Puget Sound region, Washington, USA, to examine the effect of development configuration on birds. We hypothesized that lower-intensity conservation developments, compared to higher-intensity planned community developments, would benefit the overall bird community, as well as native forest birds (avoiders of human development) and avian generalist species (adapters to human development), but that higher-intensity planned community developments would benefit synanthropic species (exploiters of human development). We fit single-species and multi-species occupancy models to test these hypotheses. Consistent with our hypotheses, we found that a greater proportion of the overall community, avoiders, and adapters occupied lower-intensity conservation developments compared to higher-intensity planned community developments. However, we did not detect an effect of development type on the exploiter guild, and we found that species in the exploiter guild are variable in their response to the configuration of suburban developments. We also hypothesized that human tolerance guilds would be a useful predictor of individual species responses to development type. This hypothesis was somewhat supported: we found that, for avoiders, 87% of species in the guild had the same response to development type as the overall guild; for adapters, 63% had the same response as the overall guild, and for exploiters, only 44% had the same response as the overall guild. Our results indicate that the configuration of suburban developments can have a meaningful impact on bird communities, particularly on those species that are most sensitive to any level of development.

Washington

The U.S. Geological Survey, the U.S. Department of Defense, and the U.S. Intelligence Community—100 years of mapping and remote sensing collaboration, 1879–1979

Introduction The U.S. Geological Survey (USGS)—a Federal civilian agency—and U.S. military and intelligence agencies collaborate on mapping and remote sensing and have since the establishment of the USGS. The organizations exchange data and information and share technology to further their respective missions in service to the American people. Often referred to as examples of “good government” or “whole of government,” the collaboration avoids costly duplication and maximizes time and effort for the government sectors. Collaboration between these sectors started with the original mapping of the United States and evolved to include remote sensing after the advent of aerial photography and satellite imagery.

Circular

Chemical evolution and estimated flow velocity of water in the Trinity Aquifer, south-central Texas

Three permeable zones with varying lithology and water chemistry compose the Trinity aquifer, a principal source of water in the 5,500- square-mile study area in south-central Texas. The upper permeable zone locally yields small quantities of water to wells and was not included in this study. The middle permeable zone primarily is composed of limestone with minor amounts of dolostone. Terrigenous sand and marine limestone, with minor amounts of dolostone, are the principal lithologic units in the lower permeable zone. Dissolved solids concentrations range from 329 to 1,820 milligrams per liter in water samples from the middle permeable zone and from 518 to 3,030 milligrams per liter in water samples from the lower permeable zone. Principal hydrochemical facies in the middle permeable zone are calcium magnesium bicarbonate and calcium magnesium sulfate. Hydrochemical facies in ground-water samples from the lower permeable zone vary. Tritium concentrations as large as 5.3 tritium units in the southeastern part of the study area are indicative of relatively recent recharge. Results of a geochemical mass balance simulation along a flowpath in the middle permeable zone indicate a mass transfer of 4.25 millimoles per liter of dolomite dissolved, 5.74 millimoles per liter of gypsum dissolved, 0.46 millimole per liter of sodium chloride dissolved, 8.07 millimoles per liter of calcite precipitated, and 0.67 millimole per liter of calcium-for-sodium cation exchange between solid and aqueous phases. These results support dedolomitization as a principal chemical process in the middle permeable zone of the Trinity aquifer. Results of a simulation along a flowpath in the lower permeable zone indicate a mass transfer of 0.41 millimole per liter of dolomite dissolved, 0.001 millimole per liter of gypsum dissolved, 9.58 millimoles per liter of sodium chloride dissolved, 1.09 millimoles per liter of calcite precipitated, and 1.11 millimoles per liter of sodium-forcalcium cation exchange between solid and aqueous phases. Lower permeable zone processes indicate sodium chloride dissolution, dedolomitization, and cation exchange. Ground-water-flow velocities determined from adjusted carbon-14 ages, calculated using NETPATH, for selected flowpaths in the middle and lower permeable zones were about 1.7 feet per year and less than about 4.4 feet per year, respectively.

Texas

U.S.-Mexico Borderland & vegetation community map

People on both sides of the United States-Mexico border need a high-resolution, binational vegetation community map that spans the entire United States-Mexico borderlands. Traditionally, mapping efforts in this region were impeded by complex logistics related to the international border, differing national needs and plans, and resource allocations and priorities. To address this need, scientists from the U.S. Geological Survey (USGS) Southwest Biological Science Center partnered with the Sonoran Joint Venture, the U.S. Fish and Wildlife Service (FWS) Migratory Bird Program, data engineers from the Department of Biosystems Engineering at the University of Arizona, and collaborators from the Wildlands Network, the Borderlands Program to produce the first prototype land cover map within the overlapping Mojave Desert, Sonoran Desert, and the North American Bird Conservation Initiative’s Bird Conservation Region 33 (BCR33) using Landsat satellite data . BCR33 is an area of high biodiversity, providing habitat for bird species of concern and other wildlife. The land cover map supports FWS recovery plan efforts related to conservation planning activities for many species, including Yellow-billed Cuckoo ( Coccyzus americanus ), Cactus Ferruginous Pygmy-Owl ( Glaucidium brasilianum cactorum ), Southwestern Willow Flycatcher ( Empidonax traillii extimus ), Yuma Ridgway’s Rail ( Rallus obsoletus yumanensis ), Bendire’s thrasher ( Toxostoma bendirei ), LeConte’s thrasher ( Toxostoma lecontei ), Masked Bobwhite ( Colinus virginianus ridgwayi ), jaguar ( Panthera onca ), and endangered plants such as Bartram’s stonecrop ( Graptopetalum bartramii ) and the Pima pineapple cactus ( Coryphantha robustispina ssp. robustispina ). In 2024, a Phase-II map for the full BCR33 region was completed, increasing the understanding of the binational nature of natural communities. The published map and associated paper can be found here .

Borderland

Seismic moment and local magnitude scales in Ridgecrest, CA from the SCEC/USGS Community Stress Drop Validation Study

We illustrate the systematic difference between moment magnitude and local magnitude caused by underlying earthquake source physics, using seismic moments submitted to the Statewide California Earthquake Center/United States Geological Survey Community Stress Drop Validation Study 2019 Ridgecrest data set. While the relationship between seismic moment and moment magnitude ( M or M w ) of log 10 ( M 0 ) ~ 1.5* M is uniformly valid for all earthquake sizes by definition (Hanks and Kanamori, 1979), the relationship between local magnitude M L and moment is itself magnitude dependent. For moderate events, ~3< M < ~6, M and M L are coincident; for earthquakes smaller than ~3, M L ~ 1.0 log 10 M 0 (Hanks and Boore, 1984). This is a physical consequence of the corner frequency fc becoming larger than the upper frequency of observation and implies that M L and M differ systematically by a factor of 1.5 for these small events. While this idea is not new, we propose a new, continuous relationship between local magnitude and moment, for magnitudes 2 to 6 which extrapolates to smaller and larger magnitudes, applicable to southern California specific to the Ridgecrest region. We make use of the plethora of seismic moments as submitted by many participants of the Community Stress Drop study, compared to the Southern California Seismic Network (SCSN) catalog magnitudes. Overall, the seismic moments in the Community Study recover moment magnitude well, so we use our new M L - M 0 to convert M L to M , refining the SCSN operational M Lr scale. This systematic difference of 50% in slope between local and moment magnitude at small magnitudes has implications for spectral stress drop estimates, earthquake ground motion modeling, as well as other magnitude scales and earthquake occurrence statistics.

California

Biogeochemical and community ecology responses to the wetting of non-perennial streams

Transitions between dry and wet hydrologic states are the defining characteristic of non-perennial rivers and streams, which constitute the majority of the global river network. Although past work has focused on stream drying characteristics, there has been less focus on how hydrology, ecology and biogeochemistry respond and interact during stream wetting. Wetting mechanisms are highly variable and can range from dramatic floods and debris flows to gradual saturation by upwelling groundwater. This variation in wetting affects ecological and biogeochemical functions, including nutrient processing, sediment transport and the assembly of biotic communities. Here we synthesize evidence describing the hydrological mechanisms underpinning different types of wetting regimes, the associated biogeochemical and organismal responses, and the potential scientific and management implications for downstream ecosystems. This combined multidisciplinary understanding of wetting dynamics in non-perennial streams will be key to predicting and managing for the effects of climate change on non-perennial ecosystems.

Nature Water

A decade of shaking in the Garden City: The dynamics of preparedness, perceptions, and beliefs in Canterbury, New Zealand, and implications for earthquake information

This study explored earthquake preparedness over time - before, during, and 10 years after the Canterbury Earthquake Sequence (CES) in Aotearoa New Zealand (NZ). Surveys of Canterbury residents were conducted in 2009, 2013 and again in 2021, using variables derived from Community Engagement Theory (CET). The surveys measured earthquake perceptions and beliefs, participation and engagement, and preparedness actions. Results were compared across the three samples. Findings indicate that perceptions and beliefs (e.g. risk perception, outcome expectancy beliefs), and types of preparedness actions taken (e.g. collection of survival items, structural preparedness, community and agency relationships), differed over time, depending on people’s experiences before, during, and after the CES. For example, during and after the CES people were more likely to believe that preparing provided a benefit to daily life, but less likely to think it could reduce property damage, perhaps due to people’s experiences of disruption and damage during the earthquakes. An understanding of such dynamics can assist with the provision and timing of risk and preparedness information. This study highlights the importance of providing applicable and actionable preparedness information, that is relevant to people’s experiences, throughout an earthquake sequence. Such information might evolve and change in focus over time depending on risks and needs. Focus could also be given to information that builds peoples beliefs and capacities to undertake preparedness in evolving situations. Understanding preparedness in the context of different experiences and timeframes is useful in helping update models such as the CET, where the dynamics of time might be better incorporated.

Canterbury

Tapwater exposures, residential risk, and mitigation in a PFAS-impacted-groundwater community

Tapwater (TW) safety and sustainability are priorities in the United States. Per/polyfluoroalkyl substance(s) (PFAS) contamination is a growing public-health concern due to prolific use, widespread TW exposures, and mounting human-health concerns. Historically-rural, actively-urbanizing communities that rely on surficial-aquifer private wells incur elevated risks of unrecognized TW exposures, including PFAS, due to limited private-well monitoring and contaminant-source proliferation in urbanizing landscapes. Here, a broad-analytical-scope TW-assessment was conducted in a hydrologically-vulnerable, Mississippi River alluvial-island community, where PFAS contamination of the shallow-alluvial drinking-water aquifer has been documented, but more comprehensive contaminant characterization to inform decision-making is currently lacking. In 2021, we analyzed 510 organics, 34 inorganics, and 3 microbial groups in 11 residential and community locations to assess (1) TW risks beyond recognized PFAS issues, (2) day-to-day and year-to-year risk variability, and (3) suitability of the underlying sandstone aquifer as an alternative source to mitigate TW-PFAS exposures. Seventy-six organics and 25 inorganics were detected. Potential human-health risks of detected TW exposures were explored based on cumulative benchmark-based toxicity quotients ( ∑ TQ ). Elevated risks ( ∑ TQ ≥ 1) from organic and inorganic contaminants were observed in all alluvial-aquifer-sourced synoptic samples but not in sandstone-aquifer-sourced samples. Repeated sampling at 3 sites over 52–55 h indicated limited variability in risk over the short-term. Comparable PFAS-specific ∑ TQ for spatial-synoptic, short-term (3 days) temporal, and long-term (3 years quarterly) temporal samples indicated that synoptic results provided useful insight into the risks of TW-PFAS exposures at French Island over the long-term. No PFAS detections in sandstone-aquifer-sourced samples over a 3 year period indicated no PFAS-associated risk and supported the sandstone aquifer as an alternative drinking-water source to mitigate community TW-PFAS exposures. This study illustrated the importance of expanded contaminant monitoring of private-well TW, beyond known concerns (in this case, PFAS), to reduce the risks of a range of unrecognized contaminant exposures.

Wisconsin

Prioritizing resource protection and understanding potential susceptibility of springs to surficial changes in a low-temperature geothermal system

Geothermal systems are vulnerable to changes in water budget and composition, requiring science-based management. This study uses a dataset of spring water temperatures, time series of groundwater residence time tracers (tritium and carbon-14), and stable isotopes of water to understand geothermal flow in a low-temperature geothermal system in north west Colorado, United States (Steamboat Springs). The geothermal system is bisected by the Yampa River, necessitating a stream mass balance approach to quantify total discharge. Time series analysis of water temperature data provides a ranked list of features more susceptible to surficial changes, which is corroborated using time series of tritium which indicate spatially distinct patterns of mixing between modern and pre-modern groundwater. All springs contain a portion of pre-modern groundwater that is thousands to tens of thousands of years old, a period coinciding with melting of extensive Pleistocene glaciers that was likely one of the recharge sources to the geothermal system. Stream mass balance indicates that greater than 80% of the total geothermal discharge is derived from diffuse or small springs, highlighting the extensive nature of the geothermal outflow zone and the association with local geologic structures. This study provides baseline data to support management of the Steamboat Springs geothermal system and indicates the utility of these approaches in developing science-based geothermal management.

Colorado

Developing, testing, and communicating earthquake forecasts: Current practices and future directions

While deterministically predicting the time and location of earthquakes remains impossible, earthquake forecasting models can provide estimates of the probabilities of earthquakes occurring within some region over time. To enable informed decision-making of civil protection, governmental agencies, or the public, Operational Earthquake Forecasting (OEF) systems aim to provide authoritative earthquake forecasts based on current earthquake activity in near-real time. Establishing OEF systems involves several nontrivial choices. This review captures the current state of OEF worldwide and analyzes expert recommendations on the development, testing, and communication of earthquake forecasts. An introductory summary of OEF-related research is followed by a description of OEF systems in Italy, New Zealand, and the United States. Combined, these two parts provide an informative and transparent snapshot of today's OEF landscape. In Section 4, we analyze the results of an expert elicitation that was conducted to seek guidance for the establishment of OEF systems. The elicitation identifies consensus and dissent on OEF issues among a non-representative group of 20 international earthquake forecasting experts. While the experts agree that communication products should be developed in collaboration with the forecast user groups, they disagree on whether forecasting models and testing methods should be user-dependent. No recommendations of strict model requirements could be elicited, but benchmark comparisons, prospective testing, reproducibility, and transparency are encouraged. Section 5 gives an outlook on the future of OEF. Besides covering recent research on earthquake forecasting model development and testing, upcoming OEF initiatives are described in the context of the expert elicitation findings.

Reviews of Geophysics

The effect of temperature, flow, density and disease on smallmouth bass (Micropterus dolomieu) early life growth rate

Individual growth rates of fish are influenced by gradually changing environmental conditions and rapid-onset events. However, there is a paucity of information on drivers of spatiotemporal variability in growth rates of fishes across large spatial extents. Using a 36-year dataset of smallmouth bass ( Micropterus dolomieu ) length-at-age data across 10 river reaches in Pennsylvania, USA ( n = 54,068 individuals), we estimated annual early life growth rates (mm year −1 ) and assessed the effects of relative abundance, summer temperature, summer streamflow rate and a disease outbreak on growth rate. We found that temperature had a positive effect on growth rate, varied spatially, and had a stronger effect in diseased reaches. There was also evidence for an impact of disease on early life growth through density-dependent mechanisms, with growth rates increasing from ∼85 to 101 mm·year −1 following disease-related decreases in abundance. This study adds to our understanding of the factors that shape early life growth, including rapid-onset events like disease which can have immediate and lasting effects on growth rate trajectory.

Pennsylvania

Cascadia Subduction Zone science: Call for the next generation community seismic velocity model

The Cascadia subduction zone (CSZ) hosts major seismic and tsunami hazards, yet key questions persist about the relationship between margin structure, fluid distribution, episodic tremor and slip, shallow megathrust behavior, shaking and tsunamigenesis, and the resulting hazard estimates. Addressing these problems requires an empirically grounded, three‐dimensional seismic velocity model to illuminate subsurface structure and properties and to provide a basis for geophysical studies such as earthquake simulations and ground‐motion estimation. In May 2024, the National Science Foundation‐funded Cascadia Region Earthquake Science Center (CRESCENT) community velocity model (CVM) working group, with U.S. Geological Survey and regional partners, convened a workshop to identify priorities for such a model. Participants emphasized the features necessary for addressing key science questions, including implementing findability, accessibility, interoperability, and reusability (FAIR) access, capturing along‐strike and along‐dip structural heterogeneity, resolving shallow offshore–onshore structure, constraining elastic properties and quantifying their uncertainties for numerical wave propagation simulations, their validation benchmarks, and supporting associated accurate earthquake ground‐motion simulations and hazard assessments. This article describes the priorities defined in the workshop, and a description of how, guided by these needs, CRESCENT plans to develop multiple generations of a CVM to advance CSZ science and improve seismic and tsunami hazard modeling across the Pacific Northwest. The CVM will span the CSZ from the surface to ∼100 km depth, offshore and east of the Cascades into Idaho (∼132°–110° W) and the southern and northern tectonic regime transitions (∼36°–52° N) to capture the entire tectonic system as well as its surroundings.

Cascadia Subduction Zone

Overview of The SCEC/USGS Community Stress Drop Validation Study using the 2019 Ridgecrest earthquake sequence

We present initial findings from the ongoing Community Stress Drop Validation Study to compare spectral stress‐drop estimates for earthquakes in the 2019 Ridgecrest, California, sequence. This study uses a unified dataset to independently estimate earthquake source parameters through various methods. Stress drop, which denotes the change in average shear stress along a fault during earthquake rupture, is a critical parameter in earthquake science, impacting ground motion, rupture simulation, and source physics. Spectral stress drop is commonly derived by fitting the amplitude‐spectrum shape, but estimates can vary substantially across studies for individual earthquakes. Sponsored jointly by the U.S. Geological Survey and the Statewide (previously, Southern) California Earthquake Center our community study aims to elucidate sources of variability and uncertainty in earthquake spectral stress‐drop estimates through quantitative comparison of submitted results from independent analyses. The dataset includes nearly 13,000 earthquakes ranging from M 1 to 7 during a two‐week period of the 2019 Ridgecrest sequence, recorded within a 1° radius. In this article, we report on 56 unique submissions received from 20 different groups, detailing spectral corner frequencies (or source durations), moment magnitudes, and estimated spectral stress drops. Methods employed encompass spectral ratio analysis, spectral decomposition and inversion, finite‐fault modeling, ground‐motion‐based approaches, and combined methods. Initial analysis reveals significant scatter across submitted spectral stress drops spanning over six orders of magnitude. However, we can identify between‐method trends and offsets within the data to mitigate this variability. Averaging submissions for a prioritized subset of 56 events shows reduced variability of spectral stress drop, indicating overall consistency in recovered spectral stress‐drop values.

California

Magnitude, depth and methodological variations of spectral stress drop within the SCEC/USGS Community Stress Drop Validation Study using the 2019 Ridgecrest Earthquake Sequence

We present the first ensemble analysis of the 56 different sets of results submitted to the ongoing Community Stress Drop Validation Study using the 2019 Ridgecrest, California, earthquake sequence. Different assumptions and methods result in different estimation of the source contribution to recorded seismograms, and hence to the source parameters (principally corner frequency, f c ⁠ , spectral stress drop, Δσ, and seismic moment, M 0 ⁠ ) obtained from modeling calculated source spectra. For earthquakes smaller than magnitude (M) 2.5 there is negligible correlation between the f c values obtained by different studies, implying that no present method is reliable using available data. For larger magnitude events, correlation between f c measurements of different studies, within even a small M range is always higher than spectral ⁠Δσ , because the f c measurements simply reflect the underlying physical decrease in f c with increasing M. We model the observed trends of submitted f c with both magnitude and depth. Most methods report an increase in spectral Δσ with M, although a magnitude‐invariant spectral Δσ is within the confidence limits. The depth dependence is smaller and depends on whether a study allows attenuation to vary with source depth; a combination of depth‐dependent attenuation correction, and depth‐dependent shear‐wave velocity can compensate for reported depth trends. We model the submitted values to remove differing M and depth variation to investigate the relative interevent variability. We find consistent relative variation between individual events, and also lower relative spectral Δσ in the northwest of the aftershock sequence, and higher on the cross fault and in the region of main fault intersection. This large‐scale comparison implies that absolute spectral Δσ estimates are dependent on the methods used; studies of different regions or using different methods should not be directly compared and improved constraints on path and site corrections are needed to resolve these absolute spectral Δσ differences.

California

SCEC/USGS Community Stress Drop Validation Study: How spectral fitting approaches influence measured source parameters

Spectral source parameters used to estimate an earthquake’s stress drop (Δσ) can vary significantly across measurement approaches. The Statewide California Earthquake Center/U.S. Geological Survey Community Stress-Drop Validation Study was initiated to compare source parameter estimates, focusing initially on a dataset from the 2019 Ridgecrest earthquake sequence. As part of that validation effort, here we focus on one potential source of uncertainty: whether spectral fitting approaches alone, applied to a common set of spectra from the 2019 Ridgecrest sequence result in different source parameter estimates. By using a common set of benchmark spectra analyzed across a consistent frequency band of 1–40 Hz, we eliminate many sources of variability. A subgroup of validation study participants volunteered to estimate the low-frequency displacement (Ω0) and corner frequency ( f c ) by fitting a smooth function to benchmark displacement spectra. Participants used linear- or log-sampled spectra, assumed a Brune or Boatwright spectral model, and applied different misfit criteria. We compare 17 approaches used to estimate Ω0, f c , and Δσ for 54 earthquake spectra. Our results reveal that 35% of events have Δσ estimates within a factor of two, whereas others exhibit variations exceeding an order of magnitude. The variability in Ω0 and f c can largely be attributed to whether a spectrum is consistent with the smooth function of an idealized simple crack model. The trade-off between Ω0 and f c may be more pronounced when using linearly sampled spectra, as higher frequency spectral bumps control the fits. As expected, methods that assumed a Boatwright model tended to have lower Ω0 and somewhat higher f c compared to those assuming a Brune model, although resulting Δσ estimates are similar. When compared to the overall validation study results, the fitting approach alone may account for between 5% and 90% (25% on average) of the total variability in spectral Δσ.

California

Potentially toxic elements in wild Agassiz’s desert tortoises: Tissue concentrations and association with disease

Background: Desert tortoise ( Gopherus agassizii ) populations have continued to decline due to infectious and other diseases, predation, and habitat alteration. The potential contribution of minerals and heavy metals to tortoise health and susceptibility to disease remains uncertain. Objective: The objective of this study was to evaluate the results of elemental analysis of trace minerals and macrominerals in scute keratin, kidney, and liver tissue from ill and dying desert tortoises salvaged for necropsy between 1993 and 2000. Methods: Salvaged tortoises were categorized by age (adult, juvenile), geographic location, and primary disease based on necropsy findings. A subset of tortoises that were injured or killed by vehicular trauma or predation but with no notable pathologic abnormalities were used as controls. A panel of 21 trace minerals and 6 macrominerals was analyzed in scute keratin, kidney, and liver tissue samples by inductively-coupled plasma spectrometry and atomic absorption spectrophotometry. Results: Necropsies were done on 46 tortoises, including 9 juveniles salvaged from 5 regions in the Colorado and Mojave deserts of California. Primary diseases were cutaneous dyskeratosis (n=9), infection/ inflammation (n=8), malnutrition (n=7), mycoplasmosis (n=5), and urolithiasis (n=3); 14 tortoises were classified as controls. Concentrations of elements differed significantly by tissue, age, desert region, and disease (P < 0.05). Tortoises with cutaneous dyskeratosis had significantly higher Se concentrations, primarily in keratin and liver, than tortoises with other diseases (P < 0.001). Juveniles were more likely than adults to have high Pb, Sn, and Zn levels (P < 0.05). All tortoises had detectable levels of more than one potentially toxic heavy metal, including As, Cd, Cr, Hg, Ni, Pb, Sn and V. Conclusions: Potentially toxic elements are frequently found in tissue from tortoises in desert regions of California, with significantly higher concentrations in diseased tortoises. Metal exposure from soils, mining, historic and ongoing military activities, and other human activities could increase susceptibility to disease in desert tortoises.

California