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Predictive understanding of stream salinization in a developed watershed using machine learning

Stream salinization is a global issue, yet few models can provide reliable salinity estimates for unmonitored locations at the time scales required for ecological exposure assessments. Machine learning approaches are presented that use spatially limited high-frequency monitoring and spatially distributed discrete samples to estimate the daily stream-specific conductance across a watershed. We compare the predictive performance of space- and time-unaware Random Forest models and space- and time-aware Recurrent Graph Convolution Neural Network models (KGE: 0.67 and 0.64, respectively) and use explainable artificial intelligence methods to interpret model predictions and understand salinization drivers. These models are applied to the Delaware River Basin, a developed watershed with diverse land uses that experiences anthropogenic salinization from winter deicer applications. These models capture seasonality for the winter first flush of deicers, and the streams with elevated predictions correspond well with indicators of deicer application. This result suggests that these models can be used to identify potential salinity-impaired streams for winter best management practices. Daily salinity predictions are driven primarily by land cover (urbanization) trends that may represent anthropogenic salinization processes and weather at time scales up to three months. Such modeling approaches are likely transferable to other watersheds and can be applied to further understand salinization risks and drivers.

Environmental Science and Technology

Using imaging spectroscopy and elevation in machine learning to estimate soil salinity in intermittently tidal wetlands

Coastal soil salinization patterns are changing due to drought, sea level rise (SLR), and changing freshwater inflow. These changes are expected to impact coastal wetland plant health and ecosystem function, such as changes to biomass and productivity. These impacts have led to greater interest in how we monitor soil salinization across spatial and temporal scales. Remote sensing is a promising tool for estimating soil salinity at the spatial scales required for decision making by land managers. However, the development of a remote sensing estimation approach for wetland soil salinity must account for two factors: (1) the high spatial and temporal heterogeneity of coastal wetlands and (2) the fact that soil salinity is the result of multiple historical land use, hydrological, and geomorphic processes. In spring 2022, a combined airborne-field campaign, known as SHIFT, collected a weekly time series of airborne visible to shortwave infrared (VSWIR) image spectroscopy data. This dataset provides a unique opportunity to assess the application of fine spatial (5 m) and temporal (weekly) resolution VSWIR data to estimate root zone soil salinity; when combined with environmental variables such as elevation, these data can account for some of these factors. In this study, we utilized VSWIR and elevation datasets in a random forest regression to predict and map soil salinity in an intermittently tidal estuary, Devereux Slough, located in Santa Barbara County, California. The final model combined spectral indices with elevation to better capture soil salinity dynamics despite lower correlation ( r = 0.85) than solely using elevation ( r = 0.92). This research demonstrates the utility of remote sensing datasets, namely, elevation and the modified Anthocyanin Reflectance Index (mARI), for predicting root zone soil salinity in intermittently tidal coastal wetlands. These findings are an important step in advancing coastal remote sensing by creating a gridded salinity dataset that can be used for salinity monitoring and other coastal applications, such as modeling change in vegetation communities or ecosystems facing the impacts of climatic variability and change.

California

Application of a lipopolysaccharide (LPS)-stimulated mitogenesis assay in smallmouth bass (Micropterus dolomieu) to augment wild fish health studies

The utility of a functional immune assay for smallmouth bass ( Micropterus dolomieu ) lymphocyte mitogenesis was evaluated. Wild populations in the Potomac River have faced disease and mortality with immunosuppression from exposure to chemical contaminants a suspected component. However, a validated set of immune parameters to screen for immunosuppression in wild fish populations is not available. Prior to use in ecotoxicology studies, ancillary factors influencing the mitogenic response need to be understood. The assay was field-tested with fish collected from three sites in West Virginia as part of health assessments occurring in spring (pre-spawn; April–May) and fall (recrudescence; October–November). Anterior kidney leukocytes were exposed to lipopolysaccharide (LPS) from E.coli O111:B4 or mitogen-free media and proliferation was measured using imaging flow cytometry with advanced machine learning to distinguish lymphocytes. An anti-smallmouth bass IgM monoclonal antibody was used to identify IgM+ lymphocytes. Lymphocyte mitogenesis, or proliferative responses, varied by site and season and positively and negatively correlated with factors such as sex, age, tissue parasites, and macrophage aggregates. Background proliferation of IgM− lymphocytes was negatively correlated to LPS-induced proliferation in both seasons at all sites, but only in spring for IgM+ lymphocytes. The results demonstrate that many factors, in addition to chemical contaminants, may influence lymphocyte proliferation.

Virginia, West Virginia

Approaching the upper boundary of driver-response relationships: Identifying factors using a novel framework integrating quantile regression with interpretable machine learning

The identification of factors that may be forcing ecological observations to approach the upper boundary provides insight into potential mechanisms affecting driver-response relationships, and can help inform ecosystem management, but has rarely been explored. In this study, we propose a novel framework integrating quantile regression with interpretable machine learning. In the first stage of the framework, we estimate the upper boundary of a driver-response relationship using quantile regression. Next, we calculate “potentials” of the response variable depending on the driver, which are defined as vertical distances from the estimated upper boundary of the relationship to observations in the driver-response variable scatter plot. Finally, we identify key factors impacting the potential using a machine learning model. We illustrate the necessary steps to implement the framework using the total phosphorus (TP)-Chlorophyll a (CHL) relationship in lakes across the continental US. We found that the nitrogen to phosphorus ratio (N:P), annual average precipitation, total nitrogen (TN), and summer average air temperature were key factors impacting the potential of CHL depending on TP. We further revealed important implications of our findings for lake eutrophication management. The important role of N:P and TN on the potential highlights the co-limitation of phosphorus and nitrogen and indicates the need for dual nutrient criteria. Future wetter and/or warmer climate scenarios can decrease the potential which may reduce the efficacy of lake eutrophication management. The novel framework advances the application of quantile regression to identify factors driving observations to approach the upper boundary of driver-response relationships.

Frontiers of Environmental Science & Engineering

Chlorophyll a in lakes and streams of the United States (2005–2022)

The concentration of chlorophyll a in phytoplankton and periphyton represents the amount of algal biomass. We compiled an 18-year record (2005–2022) of pigment data from water bodies across the United States (US) to support efforts to develop process-based, machine learning, and remote sensing models for prediction of harmful algal blooms (HABs). To our knowledge, this dataset of nearly 84,000 sites and over 1,374,000 pigment measurements is the largest compilation of harmonized discrete, laboratory-extracted chlorophyll data for the US. These data were compiled from the Water Quality Portal (WQP) and previously unpublished U.S. Geological Survey’s National Water Quality Laboratory (NWQL) data. Data were harmonized for reporting units, pigment type, duplicate values, collection depth, site name, negative values, and some extreme values. Across the country, data show great variation by state in sampling frequency, distribution, and methods. Uses for such data include the calibration of models, calibration of field sensors, examination of relationship to nutrients and other drivers, evaluation of temporal trends, and other applications addressing local to national scale concerns.

Nature Scientific Data

Using landscape genomics to delineate future adaptive potential for climate change in the Yosemite toad (Anaxyrus canorus)

An essential goal in conservation biology is delineating population units that maximize the probability of species persisting into the future and adapting to future environmental change. However, future-facing conservation concerns are often addressed using retrospective patterns that could be irrelevant. We recommend a novel landscape genomics framework for delineating future “Geminate Evolutionary Units” (GEUs) in a focal species: (1) identify loci under environmental selection, (2) model and map adaptive conservation units that may spawn future lineages, (3) forecast relative selection pressures on each future lineage, and (4) estimate their fitness and likelihood of persistence using geo-genomic simulations. Using this process, we delineated conservation units for the Yosemite toad ( Anaxyrus canorus ), a U.S. federally threatened species that is highly vulnerable to climate change. We used a genome-wide dataset, redundancy analysis, and Bayesian association methods to identify 24 candidate loci responding to climatic selection ( R 2 ranging from 0.09 to 0.52), after controlling for demographic structure. Candidate loci included genes such as MAP3K5, involved in cellular response to environmental change. We then forecasted future genomic response to climate change using the multivariate machine learning algorithm Gradient Forests. Based on all available evidence, we found three GEUs in Yosemite National Park, reflecting contrasting adaptive optima: YF-North (high winter snowpack with moderate summer rainfall), YF-East (low to moderate snowpack with high summer rainfall), and YF-Low-Elevation (low snowpack and rainfall). Simulations under the RCP 8.5 climate change scenario suggest that the species will decline by 29% over 90 years, but the highly diverse YF-East lineage will be least impacted for two reasons: (1) geographically it will be sheltered from the largest climatic selection pressures, and (2) its standing genetic diversity will promote a faster adaptive response. Our approach provides a comprehensive strategy for protecting imperiled non-model species with genomic data alone and has wide applicability to other declining species.

California

New software methods in radar ornithology using WSR-88D weather data and potential application to monitoring effects of climate change on bird migration

Radar ornithology has provided tools for studying the movement of birds, especially related to migration. Researchers have presented qualitative evidence suggesting that birds, or at least migration events, can be identified using large broad scale radars such as the WSR-88D used in the NEXRAD weather surveillance system. This is potentially a boon for ornithologists because such data cover a large portion of the United States, are constantly being produced, are freely available, and have been archived since the early 1990s. A major obstacle to this research, however, has been that identifying birds in NEXRAD data has required a trained technician to manually inspect a graphically rendered radar sweep. A single site completes one volume scan every five to ten minutes, producing over 52,000 volume scans in one year. This is an immense amount of data, and manual classification is infeasible. We have developed a system that identifies biological echoes using machine learning techniques. This approach begins with training data using scans that have been classified by experts, or uses bird data collected in the field. The data are preprocessed to ensure quality and to emphasize relevant features. A classifier is then trained using this data and cross validation is used to measure performance. We compared neural networks, naive Bayes, and k-nearest neighbor classifiers. Empirical evidence is provided showing that this system can achieve classification accuracies in the 80th to 90th percentile. We propose to apply these methods to studying bird migration phenology and how it is affected by climate variability and change over multiple temporal scales.

Conference Paper

Integration of vegetation classification with land cover mapping: Lessons from regional mapping efforts in the Americas

Aims : Natural resource management and biodiversity conservation rely on inventories of vegetation that span multiple management or political jurisdictions. However, while remote sensing data and analytical tools have enabled production of maps at increasing spatial resolution and reliability, there are limited examples where national or continental-scaled maps are produced to represent vegetation at high thematic detail. We illustrate two examples that have bridged the gap between traditional land cover mapping and modern vegetation classification. Study area : Our two case studies include national ( USA ) and continental (North and South America) vegetation and land cover mapping. These studies span conditions from subpolar to tropical latitudes of the Americas. Methods : Both case studies used a supervised modeling approach with the International Vegetation Classification ( IVC ) to produce maps that provide for greater thematic detail. Georeferenced locations for these vegetation types are used by machine learning algorithms to train a predictive model and generate a distribution map. Results : The USA LANDFIRE (Landscape Fire and Resource Management Planning Tools Project) case study illustrates how a history of vegetation-based classification and availability of key inputs can come together to generate standard map products covering more than 9.8 million km 2 that are unsurpassed anywhere in the world in terms of spatial and thematic resolution. That being said, it also remains clear that mapping at the thematic resolution of the IVC Group and finer resolution require very large and spatially balanced inputs of georeferenced samples. Even with extensive prior data collection efforts, these remain a key limitation. The NatureServe effort for the Americas - encompassing 22% of the global land surface - demonstrates methods and outputs suitable for worldwide application at continental scales. Conclusions : Continued collection of input data used in the case studies could enable mapping at these spatial and thematic resolutions around the globe.

Vegetation Classification and Survey

Impact of spectral resolution on quantifying cyanobacteria in lakes and reservoirs: A machine-learning assessment

Cyanobacterial harmful algal blooms are an increasing threat to coastal and inland waters. These blooms can be detected using optical radiometers due to the presence of phycocyanin (PC) pigments. The spectral resolution of best-available multispectral sensors limits their ability to diagnostically detect PC in the presence of other photosynthetic pigments. To assess the role of spectral resolution in the determination of PC, a large (N = 905) database of colocated in situ radiometric spectra and PC are employed. We first examine the performance of selected widely used machine-learning (ML) models against that of benchmark algorithms for hyperspectral remote sensing reflectance ( R r s ) spectra resampled to the spectral configuration of the Hyperspectral Imager for the Coastal Ocean (HICO) with a full-width at half-maximum (FWHM) of < 6 nm. Results show that the multilayer perceptron (MLP) neural network applied to HICO spectral configurations (median errors < 65%) outperforms other ML models. This model is subsequently applied to R r s spectra resampled to the band configuration of existing satellite instruments and of the one proposed for the next Landsat sensor. These results confirm that employing MLP models to estimate PC from hyperspectral data delivers tangible improvements compared with retrievals from multispectral data and benchmark algorithms (with median errors between ~73% and 126%) and shows promise for developing a globally applicable cyanobacteria measurement approach.

IEEE Transactions in Geoscience and Remote Sensing

NABat ML: Utilizing deep learning to enable crowdsourced development of automated, scalable solutions for documenting North American bat populations

Bats play crucial ecological roles and provide valuable ecosystem services, yet many populations face serious threats from various ecological disturbances. The North American Bat Monitoring Program (NABat) aims to use its technology infrastructure to assess status and trends of bat populations, while developing innovative and community-driven conservation solutions. Here, we present NABat ML , an automated machine-learning algorithm that improves the scalability and scientific transparency of NABat acoustic monitoring. This model combines signal processing techniques and convolutional neural networks (CNNs) to detect and classify recorded bat echolocation calls. We developed our CNN model with internet-based computing resources (‘cloud environment’), and trained it on >600,000 spectrogram images. We also incorporated species range maps to improve the robustness and accuracy of the model for future ‘unseen’ data. We evaluated model performance using a comprehensive, independent, holdout dataset. NABat ML successfully distinguished 31 classes (30 species and a noise class) with overall weighted-average accuracy and precision rates of 92%, and ≥90% classification accuracy for 19 of the bat species. Using a single cloud-environment computing instance, the entire model training process took <16 h. Synthesis and applications . Our convolutional neural network (CNN)-based model, NABat ML , classifies 30 North American bat species using their recorded echolocation calls with an overall accuracy of 92%. In addition to providing highly accurate species-level classification, NABat ML and its outputs are compatible with Bayesian and other statistical techniques for measuring uncertainty in classification. Our model is open-source and reproducible, enabling future implementations as software on end-user devices and cloud-based web applications. These qualities make NABat ML highly suitable for applications ranging from grassroots community science initiatives to big-data methods developed and implemented by researchers and professional practitioners. We believe the transparency and accessibility of NABat ML will encourage broad-scale participation in bat monitoring, and enable development of innovative solutions needed to conserve North American bat species.

Journal of Applied Ecology

Harmonization of a water withdrawal dataset for the conterminous United States

The U.S. Geological Survey is developing nationally consistent water-use modeling approaches to replace previous methods relying on locally specific reported and estimated data. These national assessments require datasets that incorporate water withdrawal variability across the United States and over long periods. However, source data often have unclear definitions, missing or varied units, differing temporal resolutions, varied data quality, and inconsistent formats, which hinder automation and require individualized processing. The public-supply datasets described in this paper were used in machine learning models to estimate annual and monthly public-supply water use for 2000–2020 for the conterminous United States (CONUS) and in a model to estimate public-supply deliveries. Public-supply withdrawal data were acquired for the CONUS and the District of Columbia; however, 11 states had annual data for only 1 year, and 10 states had no monthly data. Annual withdrawal data were acquired for 81% of public-supply water service areas, and monthly withdrawal data were acquired for 47% for at least 1 year from 2000 to 2020. These datasets and methods provide the most comprehensive collection of reported public-supply withdrawals to date and can be used by water-use managers, the scientific community, and the broader public. The extensive data processing described herein can be applicable to datasets representing other categories of water use.

conterminous United States

Leveraging an observed-data likelihood improves the use of machine learning labels in a Bayesian hierarchical model for bioacoustic data

Classification of massive datasets by machine learning (ML) algorithms is promising for many scientific domains, especially wildlife monitoring programs that rely on passive acoustic surveys for detecting species. However, treating ML-predicted class labels (e.g., species identity) as truth biases inferences of focal parameters within common modeling frameworks. One solution is to model the misclassification process explicitly using human-validated true-class labels for a subset of observations. Validation by experts can present a substantial bottleneck in otherwise efficient workflows that use ML predictions. Bioacoustics practitioners seek guidance on both the quantity and process for selecting ML-labeled data to validate by an expert. We derive an alternative model formulation that jointly models human-validated and ML-predicted class labels with an observed-data likelihood (ODL) and use empirically informed simulations motivated by a real-data application to explore different probability designs for selecting class labels for validation. Simulation results suggest that with smaller validation sets the ODL formulation increases computational speed and reduces estimation error compared to a default MCMC data augmentation routine. Our methodology is transferable to applications that treat predictions from classification algorithms as the response variable of interest.

Annals of Applied Statistics

Subsurface characterization and machine learning predictions at Brady Hot Springs

Subsurface data analysis, reservoir modeling, and machine learning (ML) techniques have been applied to the Brady Hot Springs (BHS) geothermal field in Nevada, USA to further characterize the subsurface and assist with optimizing reservoir management. Hundreds of reservoir simulations have been conducted in TETRAD-G and CMG STARS to explore different injection and production fluid flow rates and allocations and to develop a training data set for ML. This process included simulating the historical injection and production since 1979 and prediction of future performance through 2040. ML networks were created and trained using TensorFlow based on multilayer perceptron, long short-term memory, and convolutional neural network architectures. These networks took as input selected flow rates, injection temperatures, and historical field operation data and produced estimates of future production temperatures. This approach was first successfully tested on a simplified single-fracture doublet system, followed by the application to the BHS reservoir. Using an initial BHS data set with 37 simulated scenarios, the trained and validated network predicted the production temperature for six production wells with the mean absolute percentage error of less than 8%. In a complementary analysis effort, the principal component analysis applied to 13 BHS geological parameters revealed that vertical fracture permeability shows the strongest correlation with fault density and fault intersection density. A new BHS reservoir model was developed considering the fault intersection density as proxy for permeability. This new reservoir model helps to explore under-exploited zones in the reservoir. A data gathering plan to obtain additional subsurface data was developed; it includes temperature surveying for three idle injection wells at which the reservoir simulations indicate high bottom-hole temperatures. The collected data assist with calibrating the reservoir model. Data gathering activities are planned for the first quarter of 2021.

Nevada

A probabilistic approach to training machine learning models using noisy data

Machine learning (ML) models are increasingly popular in environmental and hydrologic modeling, but they typically contain uncertainties resulting from noisy data (erroneous or outlier data). This paper presents a novel probabilistic approach that combines ML and Markov Chain Monte Carlo simulation to (1) detect and underweight likely noisy data, (2) develop an approach capable of detecting noisy data during model deployment, and (3) interpret the reasons why a data point is deemed noisy to help heuristically distinguish between outliers and erroneous data. The new algorithm recognizes that there is no unique way to split the training data into noisy and clean data, and thus produces an ensemble of plausible splits. The algorithm successfully detected noisy data in synthetic benchmark problems with varying complexity and a real-world public supply water withdrawal dataset. The algorithm is generic and flexible, making it suitable for application across a broad range of hydrologic and environmental disciplines.

Environmental Modelling & Software

Estimating vegetation biomass and cover across large plots in shrub and grass dominated drylands using terrestrial lidar and machine learning

Terrestrial laser scanning (TLS) has been shown to enable an efficient, precise, and non-destructive inventory of vegetation structure at ranges up to hundreds of meters. We developed a method that leverages TLS collections with machine learning techniques to model and map canopy cover and biomass of several classes of short-stature vegetation across large plots. We collected high-definition TLS scans of 26 1-ha plots in desert grasslands and big sagebrush shrublands in southwest Idaho, USA. We used the Random Forests machine learning algorithm to develop decision tree models predicting the biomass and canopy cover of several vegetation classes from statistical descriptors of the aboveground heights of TLS points. Manual measurements of vegetation characteristics collected within each plot served as training and validation data. Models based on five or fewer TLS descriptors of vegetation heights were developed to predict the canopy cover fraction of shrubs (R 2 = 0.77, RMSE = 7%), annual grasses (R 2 = 0.70, RMSE = 21%), perennial grasses (R 2 = 0.36, RMSE = 12%), forbs (R 2 = 0.52, RMSE = 6%), bare earth or litter (R 2 = 0.49, RMSE = 19%), and the biomass of shrubs (R 2 = 0.71, RMSE = 175 g) and herbaceous vegetation (R 2 = 0.61, RMSE = 99 g) (all values reported are out-of-bag). Our models explained much of the variability between predictions and manual measurements, and yet we expect that future applications could produce even better results by reducing some of the methodological sources of error that we encountered. Our work demonstrates how TLS can be used efficiently to extend manual measurement of vegetation characteristics from small to large plots in grasslands and shrublands, with potential application to other similarly structured ecosystems. Our method shows that vegetation structural characteristics can be modeled without classifying and delineating individual plants, a challenging and time-consuming step common in previous methods applying TLS to vegetation inventory. Improving application of TLS to studies of shrub-steppe ecosystems will serve immediate management needs by enhancing vegetation inventories, environmental modeling studies, and the ability to train broader datasets collected from air and space.

Idaho

Application of a workflow to determine the feasibility of using simulated streamflow for estimation of streamflow frequency statistics

Streamflow records from hydrologic models are attractive for use in operational hydrology, such as a streamflow frequency analysis. The amount of bias inherent to simulated streamflow from hydrologic models is often unknown, but it is likely present in derivative products. Therefore, a workflow may help determine where streamflow frequency analysis is credibly feasible from simulated streamflow and allow for a systematic way to assess and correct for bias. The proposed workflow consists of hydrologically matching model output locations with streamflow-gauging station (stream gauge) locations, computing the desired statistic from the simulated and observed streamflow record, computing the differences between the simulated and observed statistic (i.e., the bias), and constructing generalized additive models (GAMs) from the differences to determine bias corrections. The US Geological Survey, in cooperation with the Gulf Coast Ecosystem Restoration Council and the US Environmental Protection Agency, is testing the proposed workflow on a low-streamflow frequency (LFF) analysis. Simulated streamflows for the LFF analysis were sourced from a machine-learning model that estimated daily streamflow at Level-12 hydrologic unit code (HUC12) pour points (outlets) in the southern and southeastern US for 1950–2010. The comparison data set consists of 497 stream gauges that are coincident with a HUC12 outlet. The simulated LFF statistics were being overestimated on average; thus, there are limits to using simulated streamflow for frequency analysis. The magnitude of the overprediction generally increases where no-flow conditions are common. Bias corrections determined from the GAMs decreased the magnitude of bias observed in the simulated LFF statistics on average, suggesting it is feasible to expand the operational use of simulated streamflows to frequency analyses with the proposed workflow. The proposed workflow could be advantageous to practitioners interested in leveraging existing and future simulated streamflow data sets with regional and or global coverage.

Journal of Hydrologic Engineering

Animal tracing with sulfur isotopes: Spatial segregation and climate variability in Africa likely contribute to population trends of a migratory songbird

Climatic conditions affect animals but range-wide impacts at the population level remain largely unknown, especially in migratory species. However, studying climate–population relationships is still challenging in small migrants due to a lack of efficient and cost-effective geographic tracking method. Spatial distribution patterns of environmental stable isotopes (so called ‘isoscapes’) generally overcome these limitations but none of the currently available isoscapes provide a substantial longitudinal gradient in species-rich sub-Saharan Africa. In this region, sulphur ( δ 34 S) has not been sufficiently explored on a larger scale. We developed a δ 34 S isoscape to trace animal origins in sub-Saharan Africa by coupling known-origin samples from tracked migratory birds with continental remotely sensed environmental data building on environment– δ 34 S relationships using a flexible machine learning technique. Furthermore, we link population-specific nonbreeding grounds with interannual climatic variation that might translate to breeding population trends. The predicted δ 34 S isotopic map featured east–west and coast-to-inland isotopic gradients and was applied to predict nonbreeding grounds of three breeding populations of Eurasian Reed Warblers Acrocephalus scirpaceus with two distinct migratory phenotypes. Breeding populations as well as migratory phenotypes exhibited large-scale segregation within the African nonbreeding range. These regions also differed substantially in the interannual climatic variation, with higher interannual variability in the eastern part of the range during 2001–2012. Over the same period, the eastern European breeding population seemed to have experienced a more steep decline in population size. The link between migratory patterns and large-scale climatic variability appears important to better understand population trajectories in many declining migratory animals. We believe animal tracing using sulphur isotopes will facilitate these efforts and offers manifold ecological and forensic applications in the biodiversity hotspot of sub-Saharan Africa.

Journal of Animal Ecology

Listening for extinction: Range-wide occupancy highlights critical risks and priorities for northern spotted owls

The northern spotted owl ( Strix occidentalis caurina ) continues to decline across its range, threatened by habitat disturbances and invasive barred owls ( Strix varia ). We conducted a range-wide spotted owl occupancy assessment using 2.1 million h of passive acoustic recordings from 4081 survey stations within 1027 randomly selected 5-km 2 hexagons across six physiographic regions. Applying multistate occupancy models, we estimated probabilities of landscape use (by at least one northern spotted owl) and pair occupancy (occupied by both a male and a female), while accounting for imperfect and sex-biased detection probability. Detection probability of pairs was low in most regions, primarily due to undetected females, but increased with survey effort. If management decisions can only be made when there is a high probability of detecting both sexes when they are present, then longer surveys are one approach to support the decisions. Landscape use and pair occupancy were positively associated with old-growth forest structure and topographic diversity. We also found a pronounced latitudinal gradient in spotted owl landscape use rates with estimated mean landscape use of 0.17 (SE = 0.03) and 0.16 (SE = 0.02) in the two most northern regions, and 0.54 (SE = 0.04) and 0.52 (SE = 0.05) in the two most southern regions. Pair occupancy rates were higher in southern regions (0.29, SE = 0.03, 0.43, SE = 0.05) and very low in northern regions (0.04, SE = 0.01, 0.03, SE = 0.01). In contrast to spotted owls, barred owls were detected in 90% of northern hexagons and 50% of southern hexagons, and barred owl calling intensity was over eight times higher than spotted owls. These results highlight extinction risks in several regions and suggest that management actions, such as spatially targeted barred owl control and protection of structurally complex habitats, have a narrow temporal window in which they can be effective in lowering the probability of regional extirpations. We generated predictive maps of landscape use and pair occupancy, which could help guide regional conservation strategies. This study demonstrates how passive acoustic monitoring and machine learning can be used for broad-scale ecological monitoring, identifying extinction risk thresholds, supporting adaptive management, and improving conservation outcomes for wide-ranging and elusive species that can reliably be detected through unique vocalizations.

California, Oregon, Washington