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Comparing wastewater chemicals, indicator bacteria concentrations, and bacterial pathogen genes as fecal pollution indicators

The objective of this study was to compare fecal indicator bacteria (FIB) (fecal coliforms, Escherichia coli [EC], and enterococci [ENT]) concentrations with a wide array of typical organic wastewater chemicals and selected bacterial genes as indicators of fecal pollution in water samples collected at or near 18 surface water drinking water intakes. Genes tested included esp (indicating human-pathogenic ENT) and nine genes associated with various animal sources of shiga-toxin–producing EC (STEC). Fecal pollution was indicated by genes and/or chemicals for 14 of the 18 tested samples, with little relation to FIB standards. Of 13 samples with <50 EC 100 mL −1 , human pharmaceuticals or chemical indicators of wastewater treatment plant effluent occurred in six, veterinary antibiotics were detected in three, and stx 1 or stx 2 genes (indicating varying animal sources of STEC) were detected in eight. Only the EC eae A gene was positively correlated with FIB concentrations. Human-source fecal pollution was indicated by the esp gene and the human pharmaceutical carbamazepine in one of the nine samples that met all FIB recreational water quality standards. Escherichia coli rfb O157 and stx 2c genes, which are typically associated with cattle sources and are of potential human health significance, were detected in one sample in the absence of tested chemicals. Chemical and gene-based indicators of fecal contamination may be present even when FIB standards are met, and some may, unlike FIB, indicate potential sources. Application of multiple water quality indicators with variable environmental persistence and fate may yield greater confidence in fecal pollution assessment and may inform remediation decisions

Journal of Environmental Quality↗

Microbial and viral indicators of pathogens and human health risks from recreational exposure to waters impaired by fecal contamination

Fecal indicator bacteria (FIB) (e.g., fecal coliforms, Escherichia coli , and enterococci) have been used for decades to monitor for and protect the public from waterborne pathogens from fecal contamination. However, FIB may not perform well at predicting the presence of waterborne pathogens or human health outcomes from recreational exposure to fecal-contaminated surface waters. Numerous factors can influence the relationship between FIB and pathogens or human health outcomes, including the source(s) of contamination, the type of pathogen(s) present, differences in the survival and behavior of FIB and pathogens in the wastewater conveyance and treatment process, and varying environmental conditions. As a result, different indicators, such as source-specific microbial source tracking (MST) markers and viral fecal indicators, have been used as possible surrogates to better approximate pathogen abundance and human health risks in recreational waters. The performance of these alternative indicators has been mixed, with some promise of viral indicators better approximating viral pathogens than bacterial fecal indicators, and FIB generally more closely associated with bacterial and protozoal pathogen presence than human MST markers. Many of the assays to detect and quantify fecal indicators and pathogens are polymerase chain reaction-based assays, which detect and quantify nucleic acid [deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)] sequences specific to a target of interest. Recent advances in DNA and RNA sequencing technologies may push the field toward metabarcoding approaches, where multiple targets can be detected and quantified simultaneously. Metabarcoding is currently more applicable to bacterial and protozoal assessments than viral assessments based on a lack of universal metabarcoding markers for viruses. Innovative technologies, such as biosensors and nanotechnologies, may provide more sensitive and accurate tools to detect and quantify pathogens. When a specific pathogen is of concern for a recreational water body, a practical approach in estimating the likelihood of human health outcomes is the application of quantitative microbial risk assessments (QMRAs). Quantitative microbial risk assessments can be used to model the likelihood of pathogen-specific human health outcomes from recreational exposure as a function of a surrogate indicator. Inputs for QMRAs include the ratio between the indicator to be monitored and the pathogen of interest, the concentration of the indicator, the amount of water ingested, and the likelihood of the health outcome based on the estimated amount of pathogen consumed. There are numerous unknowns about the behavior and survival of fecal indicators and pathogens in environmental waters. Developing accurate models to predict pathogen concentrations from fecal indicators in recreational waters will require a better understanding of these unknowns. Current methods and technologies for detecting and quantifying fecal indicators and pathogens are limited due to the rare and patchy nature of pathogens. Technological advances may help improve sensitivity for detecting and quantifying pathogens.

Journal of Sustainable Water in the Built Environm↗

PhyloChipTM microarray comparison of sampling methods used for coral microbial ecology

Interest in coral microbial ecology has been increasing steadily over the last decade, yet standardized methods of sample collection still have not been defined. Two methods were compared for their ability to sample coral-associated microbial communities: tissue punches and foam swabs, the latter being less invasive and preferred by reef managers. Four colonies of star coral, Montastraea annularis , were sampled in the Dry Tortugas National Park (two healthy and two with white plague disease). The PhyloChip&trade; G3 microarray was used to assess microbial community structure of amplified 16S rRNA gene sequences. Samples clustered based on methodology rather than coral colony. Punch samples from healthy and diseased corals were distinct. All swab samples clustered closely together with the seawater control and did not group according to the health state of the corals. Although more microbial taxa were detected by the swab method, there is a much larger overlap between the water control and swab samples than punch samples, suggesting some of the additional diversity is due to contamination from water absorbed by the swab. While swabs are useful for noninvasive studies of the coral surface mucus layer, these results show that they are not optimal for studies of coral disease.

Dry Tortugas National Park↗

The North American strain of viral hemorrhagic septicemia virus is highly pathogenic for laboratory-reared Pacific herring ( Clupea pallasi )

Specific-pathogen-free Pacific herring Clupea pallasi were reared in the laboratory from eggs and then challenged at 5, 9, and 13 months of age by waterborne exposure to low (10 1.5–2.5 plaque-forming units [PFU] per milliliter), medium (10 3.5–4.5 PFU/mL), or high (10 5.5–6.5 PFU/mL) levels of a North American isolate of viral hemorrhagic septicemia virus (VHSV). The fish were extremely susceptible to the virus, showing clinical disease, mortality approaching 100%, and only a limited increase in resistance with age. Mortality began 4–6 d after exposure and peaked at approximately day 7 in fish exposed to high levels of virus. Whereas the mean time to death showed a significant dose response ( P < 0.001), the percent mortality and virus titers in dead fish were generally high in all groups regardless of initial challenge dose. External signs of disease were usually limited to 1–2-mm hemorrhagic areas on the lower jaw and isthmus and around the eye, but 2 of 130 infected fish exhibited extensive cutaneous hemorrhaging. Histopathologic examination of tissues from moribund fish sampled at 2–8 d after exposure revealed multifocal coagulative necrosis of hepatocytes, diffuse necrosis of interstitial hematopoietic tissues in the kidney, diffuse necrosis of the spleen, epidermis, and subcutis, and occasional necrosis of pancreatic acinar cells. Virus titers in tissues of experimentally infected herring were first detected 48 h after exposure and peaked 6-8 d after exposure at 10 7.7 PFU/g. Fish began shedding virus at 48 h after exposure with titers in the flow-through aquaria reaching 10 2.5 PFU/mL at 4–5 d after exposure, just before peak mortality. When the water flow was turned off for 3 h, titers in the water rose to 10 3.5 PFU/mL, and the amount of virus shed by infected fish (on average, greater than 10 6.5 PFU/h per fish) appeared sufficient to sustain a natural epizootic among schooling herring. Taken together, these data suggest that VHSV could be a significant limiting factor for populations of Pacific herring.

Journal of Aquatic Animal Health↗

Nowcasting methods for determining microbiological water quality at recreational beaches and drinking-water source waters

Nowcasts are tools used to provide timely and accurate water-quality assessments of threats to drinking-water and recreational resources from fecal contamination or cyanobacterial harmful algal blooms. They use mathematical models and techniques to provide near-real-time estimates of fecal-indicator bacteria (FIB) and cyanotoxin concentrations. Techniques include logic-based thresholds, decision trees (built with machine learning), multiple linear and binary logistic regression, artificial neural networks, and process-based deterministic models. The type of site (freshwater, marine, or river) and dependent variable (FIB or cyanotoxin) dictate which explanatory variables are used in models. Nowcast systems notify the public of associated public-health risks and can also be used to manage data for FIB models; work is ongoing to incorporate cyanotoxin models into some nowcasts. The Great Lakes NowCast in the USA has been operational since 2010 and includes 25 lake beaches and one recreational river site. Examples of other operational FIB nowcasts are described for locations in the USA and around the world. In many cases, models predicted exceedances of FIB standards with accuracies as good as or better than using the previous measured FIB concentration (persistence method). Accuracy and timeliness are vital to beach management decisions that protect public health and support the local recreation-driven economy. Nowcasts benefit the public by providing estimates of water-quality conditions in near-real-time. Managers can use nowcasts at recreational and drinking-water treatment plant sites when FIB or cyanotoxins are projected to be elevated to target sample collection, to provide near-real-time recreational advisories to the public, or to preemptively optimize drinking-water treatments or change intake options to mitigate possible adverse effects on drinking-water quality.

Journal of Microbiological Methods↗

Florfenicol residues in Rainbow Trout after oral dosing in recirculating and flow-through culture systems

Aquaflor is a feed premix for fish containing the broad spectrum antibacterial agent florfenicol (FFC) incorporated at a ratio of 50% (w/w). To enhance the effectiveness of FFC for salmonids infected with certain isolates of Flavobacterium psychrophilum causing coldwater disease, the FFC dose must be increased from the standard 10 mg·kg −1 body weight (BW)·d −1 for 10 consecutive days. A residue depletion study was conducted to determine whether FFC residues remaining in the fillet tissue after treating fish at an increased dose would be safe for human consumption. Groups of Rainbow Trout Oncorhynchus mykiss (total n = 144; weight range, 126–617 g) were treated with FFC at 20 mg·kg −1 BW·d −1 for 10 d in a flow-through system (FTS) and a recirculating aquaculture system (RAS) each with a water temperature of ∼13°C. The two-tank RAS included a nontreated tank containing 77 fish. Fish were taken from each tank (treated tank, n = 16; nontreated tank, n = 8) at 6, 12, 24, 48, 72, 120, 240, 360, and 480 h posttreatment. Florfenicol amine (FFA) concentrations (the FFC marker residue) in skin-on fillets from treated fish were greatest at 12 h posttreatment (11.58 μg/g) in the RAS and were greatest at 6 h posttreatment (11.09 μg/g) in the FTS. The half-lives for FFA in skin-on fillets from the RAS and FTS were 20.3 and 19.7 h, respectively. Assimilation of FFC residues in the fillets of nontreated fish sharing the RAS with FFC-treated fish was minimal. Florfenicol water concentrations peaked in the RAS-treated tank and nontreated tanks at 10 h (453 μg/L) and 11 h (442 μg/L) posttreatment, respectively. Monitoring of nitrite concentrations throughout the study indicated the nitrogen oxidation efficiency of the RAS biofilter was minimally impacted by the FFC treatment.

Journal of Aquatic Animal Health↗

Assessment of sediment contamination at Great Lakes Areas of Concern: the ARCS Program Toxicity-Chemistry Work Group strategy

In response to a mandate in Section 118(c)(3) of the Water Quality Act of 1987, a program called Assessment and Remediation of Contaminated Sediments (ARCS) was established. Four technical work groups were formed. This paper details the research strategy of the Toxicity-Chemistry Work Group. The Work Group's general objectives are to develop survey methods and to map the degree of contamination and toxicity in bottom sediments at three study areas, which will serve as guidance for future surveys at other locations. A related objective is to use the data base that will be generated to calculate sediment quality concentrations by several methods. The information needed to achieve these goals will be collected in a series of field surveys at three areas: Saginaw Bay (MI), Grand Calumet River (IN), and Buffalo River (NY). Assessments of the extent of contamination and potential adverse effects of contaminants in sediment at each of these locations will be conducted by collecting samples for physical characterization, toxicity testing, mutagenicity testing, chemical analyses, and fish bioaccumulation assays. Fish populations will be assessed for tumors and external abnormalities, and benthic community structure will be analyzed. A mapping approach will use low-cost indicator parameters at a large number of stations, and will extrapolate by correlation from traditional chemical and biological studies at a smaller number of locations. Sediment toxicity testing includes elutriate, pore water and whole sediment bioassays in a three-tiered framework. In addition to the regular series of toxicity tests at primary mater stations, some stations are selected for a more extensive suite of tests.

Journal of Aquatic Ecosystem Health↗

Research plan and preliminary results: A field research site for emerging contaminants in Iowa

Research has recently documented the prevalence of a wide variety of pharmaceuticals and other emerging contaminants (ECs) in streams across the United States. Wastewater treatment plants (WWTPs) have been found to be an important source and collection point of ECs to streams as many ECs are incompletely removed during treatment. To investigate the complex in-stream processes (e.g., dilution, sorption, degradation, dispersion, etc.) that can affect ECs following their input from a WWTP and determining if such input is having an effect on the aquatic ecosystem requires the integration of multi-disciplinary efforts at a carefully selected field site. Preliminary work has identified an 8-km reach of Fourmile Creek in central Iowa as an ideal research site to investigate such important research questions pertaining to ECs. Unique aspects of Fourmile Creek included: (1) it single source effluent-dominated stream, (2) background data document the input of a wide variety of ECs from WWTP discharge, (3) small basin size, (4) relatively simple flow system, (5) background data suggest that undefined processes are taking place decreasing the level of select ECs during stream transport, (6) the WWTP uses a treatment technology (activated sludge) typical of many towns in Iowa and the United States (7) a hydrogeologic setting of a low-gradient, small stream (average discharge less than 1.41 m&sup3;/s) in glacial drift is typical of many areas in Iowa and across the Midwest, and (8) the existence of a low-head clam approximately 2 km upstream of the WWTP outfall allowing more accurate "above WWTP" and "below WWTP" comparisons in aquatic ecosystems. Furthermore, the WWTP is scheduled to close by 2011 providing a unique opportunity to determine how stream hydrology, water chemistry and aquatic biota react to the removal of the primary source of flow and ECs in this system. This will allow a novel "before" and "after" assessment not previously available in EC research. Research to date at the site has included installation of a streamflow gauging station, dye-tracing tests (to determine water travel times), Lagrangian water-quality sampling at two flow/water temperature regimes, and sampling for ECs in bed sediment. Selected fish have been collected for analysis and identification. In addition, basic fish community and fish health assessment for different seasons and spawning conditions are being analyzed. The research "framework" is unique at Fourmile Creek for investigating the important question of how ECs are transported through the environment and if the presence of such compounds is having a deleterious effect on aquatic ecosystems.

Iowa↗

Retrospective analysis of the epidemiologic literature, 1990–2015, on wildlife-associated diseases from the Republic of Korea

To assess the status of research on wildlife diseases in the Republic of Korea (ROK) and to identify trends, knowledge gaps, and directions for future research, we reviewed epidemiologic publications on wildlife-associated diseases in the ROK. We identified a relatively small but rapidly increasing body of literature. The majority of publications were focused on public or livestock health and relatively few addressed wildlife health. Most studies that focused on human and livestock health were cross-sectional whereas wildlife health studies were mostly case reports. Fifteen diseases notifiable to the World Organisation for Animal Health were identified and 21 diseases were identified as notifiable to either the Korean Ministry of Health, Welfare, and Family Affairs or the Korean Ministry of Agriculture. Two diseases were reported as occurring as epidemics; highly pathogenic avian influenza (HPAI) and virulent Newcastle disease. Six diseases or disease agents were described in the literature as emerging including HPAI, rabies, Babesia microti , avian coronaviruses, scrub typhus, and severe fever thrombocytopenia syndrome virus. The diseases for which there were the largest number of publications were HPAI and rabies. The majority of wildlife-associated zoonotic disease publications focused on food-borne parasitic infections or rodent-associated diseases. Several publications focused on the potential of wildlife as reservoirs of livestock diseases; in particular, water deer ( Hydropotes inermis ) and wild boar ( Sus scrofa ). In contrast, there were few publications on diseases of concern for wildlife populations or research to understand the impacts of these diseases for wildlife management. Increased focus on prospective studies would enhance understanding of disease dynamics in wildlife populations. For the high-consequence diseases that impact multiple sectors, a One Health approach, with coordination among the public health, agricultural, and environmental sectors, would be important. This type of review can provide useful information for countries or regions planning or implementing national wildlife health programs.

Journal of Wildlife Diseases↗

Pesticides, polycyclic aromatic hydrocarbons, and polychlorinated biphenyls in transport in two Atlantic coastal plain tributaries and loadings to Chesapeake Bay

Concentrations of current-use pesticides, polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and organochlorine (OC) insecticides were determined above the reach of tide in the Chesterville Branch and Nanticoke River on the eastern shore of Chesapeake Bay during base-flow and storm-flow hydrologic regimes to evaluate mass transport to Chesapeake Bay. The two rivers monitored showed relatively high concentrations of atrazine, simazine, alachlor, and metolachlor in comparison to previously investigated western shore tributaries, and reflected the predominant agricultural land use in the eastern shore watersheds. The four current use pesticides showed the greatest seasonal contribution to annual loadings to tidal waters of Chesapeake Bay from the two rivers, and the relative order of annual loadings for the other contaminant classes was PAHs > PCBs > OC insecticides. Annual loadings normalized to the landscape areas of selected Chesapeake Bay watersheds showed correlations to identifiable source areas, with the highest pesticide yields (g/km2/yr) occurring in eastern shore agricultural landscapes, and the highest PAH yields derived from urban regions.

Journal of Environmental Science and Health - Part↗

Investigation of a largescale common murre (Uria aalge) mortality event in California in 2015

From August through December 2015, beachcast bird survey programs reported increased deposition of common murres ( Uria aalge ) on central and northern California beaches, but not on southern California beaches. Coastal wildlife rehabilitation centers received more than 1,000 live, stranded, and debilitated murres from Sonoma County to San Luis Obispo County during August–October. Approximately two-thirds of admitted birds were after-hatch-year birds in emaciated body condition and in various stages of molt, with extremely worn plumage. Necropsies were done on a sample ( n =35) of birds to determine the probable cause of death of beachcast carcasses. Most birds examined during necropsy were emaciated, with starvation the most likely cause of death. Birds were also tested for underlying infectious diseases at the US Geological Survey National Wildlife Health Center (NWHC) and harmful algal bloom toxins at the University of California, Santa Cruz and the National Oceanographic and Atmospheric Administration's Northwest Fisheries Science Center. Twenty-four out of 29 tested birds had detectable levels of domoic acid, and no indication of infectious disease was found. Emaciation is thought to be the cause of death for these birds, with a large warm water anomaly and harmful algal bloom playing a secondary detrimental role.

California↗

Characterizing the interface between wild ducks and poultry to evaluate the potential of transmission of avian pathogens

Background Characterizing the interface between wild and domestic animal populations is increasingly recognized as essential in the context of emerging infectious diseases (EIDs) that are transmitted by wildlife. More specifically, the spatial and temporal distribution of contact rates between wild and domestic hosts is a key parameter for modeling EIDs transmission dynamics. We integrated satellite telemetry, remote sensing and ground-based surveys to evaluate the spatio-temporal dynamics of indirect contacts between wild and domestic birds to estimate the risk that avian pathogens such as avian influenza and Newcastle viruses will be transmitted between wildlife to poultry. We monitored comb ducks ( Sarkidiornis melanotos melanotos ) with satellite transmitters for seven months in an extensive Afro-tropical wetland (the Inner Niger Delta) in Mali and characterise the spatial distribution of backyard poultry in villages. We modelled the spatial distribution of wild ducks using 250-meter spatial resolution and 8-days temporal resolution remotely-sensed environmental indicators based on a Maxent niche modelling method. Results Our results show a strong seasonal variation in potential contact rate between wild ducks and poultry. We found that the exposure of poultry to wild birds was greatest at the end of the dry season and the beginning of the rainy season, when comb ducks disperse from natural water bodies to irrigated areas near villages. Conclusions Our study provides at a local scale a quantitative evidence of the seasonal variability of contact rate between wild and domestic bird populations. It illustrates a GIS-based methodology for estimating epidemiological contact rates at the wildlife and livestock interface integrating high-resolution satellite telemetry and remote sensing data.

Inner Niger Delta↗

Volcanic eruptions and threats to respiratory health

In early May 2018, Kīlauea volcano became increasingly active, posing an increase in threat to respiratory health. The emission of gases such as sulfur dioxide from Kīlauea produces large amounts of respirable acid particles as the gases react with water vapor and sunlight, resulting in a visible haze called “vog”. Additionally, the lava lake at Kīlauea’s summit crater has fallen, leading to explosions of “ash” that have reached as high up as 30,000 feet above sea level. Finally, lava entering the Pacific Ocean boils sea water to dryness, creating thick clouds of “laze” that is filled with hydrochloric acid and tiny shards of glass. Depending on your location and wind direction and speed, vog, ash, and laze can reach hazardous levels of air pollution that are toxic to humans. This fact sheet serves to inform you of potential adverse health effects following exposure to these airborne products of volcanic activity. This outdoor air quality issue is relevant to other locations worldwide at risk for volcanic activity.

American Journal of Respiratory and Critical Care ↗

Parathion accumulation in cricket frogs and its effect on American kestrels

Adult cricket frogs (Acris crepitans) were held individually for 96 h in static systems containing initial concentrations of either 0, 0.1, 1.0, or 10 ppm parathion in 10 ml water. Mortality of cricket frogs was directly related to the parathion concentration in the water. Frogs from the 1.0- and 10-ppm groups accumulated 0.08 and 4.6 ppm parathion, respectively. One of four American kestrels (Falco sparverius) fed frogs from the 10-ppm group died from organophosphate poisoning less than 3 h after consuming five frogs. Mortality did not occur in kestrels fed frogs from the other treatment groups, which represented more environmentally realistic levels of exposure.

Journal of Toxicology and Environmental Health↗

Characterization of plasma vitellogenin and sex hormone concentrations during the annual reproductive cycle of the endangered razorback sucker

Population declines of the endangered razorback sucker Xyrauchen texanus in the Colorado River basin have been attributed to predation by and competition with nonnative fishes, habitat alteration, and dam construction. The reproductive health and seasonal variation of the reproductive end points of razorback sucker populations are currently unknown. Using nonlethal methods, we characterized the plasma hormonal fluctuations of reproductively mature female and male razorback suckers over a 12-month period in a hatchery by measuring their vitellogenin (VTG) and three sex hormones: 17&beta;-estradiol (E2), testosterone (T), and 11-ketotestosterone (KT). Fish were identified as reproductive or nonreproductive based on their body weight, VTG, and sex hormone profiles. In reproductive females, the E2 concentration increased in the fall and winter, and increases in T and VTG concentrations were generally associated with the spawning period. Mean T concentrations were consistently greater in reproductive females than in nonreproductive females, but this pattern was even more pronounced during the spawning period (spring). Consistently low T concentrations (<3 ng/mL) in adult females during the spawning period may indicate reproductive impairment. In reproductive males, spring increases in KT and T concentrations were associated with spawning; concentrations of E2 (<0.48 ng/mL) and VTG (<0.001 mg/mL) were low in males throughout the study. In addition, the E2 : KT ratio and T were the best metrics by which to distinguish female from male adult razorback suckers throughout the year. These metrics of reproductive health and condition may be particularly important to recovery efforts of razorback suckers given that the few remaining wild populations are located in a river where water quality and quantity issues are well documented. In addition to the size, age, and recruitment information currently considered in the recovery goals of this endangered species, reproductive end points could be included as recovery metrics with which to monitor seasonal trends and determine whether repatriated populations are cycling naturally.

North American Journal of Fisheries Management↗

Instream water use in the United States: water laws and methods for determining flow requirements

Water use generally is divided into two primary classes - offstream use and instream use. In offstream use, sometimes called out-of-stream or diversionary use, water is withdrawn (diverted) from a stream or aquifer and transported to the place of use. Examples are irrigated agriculture, municipal water supply, and industrial use. Each of these offstream uses, which decreases the volume of water available downstream from the point of diversion, is discussed in previous articles in this volume. Instream use, which generally does not diminish the flow downstream from its point of use, and its importance are described in this article. One of the earliest instream uses of water in the United States was to turn the water wheels that powered much of the Nation's industry in the 18th and 19th centuries. Although a small volume of water might have been diverted to a mill near streamside, that water usually was returned to the stream near the point of diversion and, thus, the flow was not diminished downstream from the mill. Over time, the generation of hydroelectric power replaced mill wheels as a means of converting water flow into energy. Since the 1920's, the generation of hydroelectric power increasingly has become a major instream use of water. By 1985, more than 3 billion acre-feet of water (3,050,000 million gallons per day) was used annually for hydropower generation (Solley and others, 1988, p. 45)-enough water to cover the State of Colorado to a depth of 51 feet. Navigation is another instream use with a long history. The Lewis and Clark expedition journals and many of Mark Twain's novels illustrate the extent to which the Nation originally depended on adequate streamfiows for basic transportation. Navigation in the 1980's is still considered to be an instream use; however, it often is based upon a stream system that has been modified greatly through channelization, diking, and construction of dams and locks. The present (1987) inland water navigation system in the conterminous United States consists of about 12,000 miles of maintained waterways, over which about 500 million tons of cargo is carried each year (U.S. Army Corps of Engineers, 1988, p. 16). Although not so widely practiced in recent years, streams have been used to dispose of raw waste products from homes, communities, and factories. This use has been discouraged by law and public policy because of public health concerns and the damage it causes to the environment. Beginning in the mid-1960's, other instream uses gained new prominence in the water-resources arena-the assertion of a legal right to a free-flowing stream for biological, recreational, and esthetic purposes. These uses themselves, however, are not new. Riverine habitat always has produced fish, and the beauty of flowing water always has evoked a strong sense of esthetic appreciation. What is new is the emerging legitimacy and awareness of these noneconomic uses under State and Federal laws and regulations. In the past, environmental uses of flowing water were ignored, for the most part, under a long-standing legal tradition that favored offstream uses and certain instream uses that had a strong economic basis. The history of instream-flow policy debate really concerns those recently recognized types of interim uses. Although the more transitional water uses have been protected by law, the recognition of other in stream uses has resulted in substantial changes in State water laws. Although methods for determining the volume of water needed for most traditional water uses are relatively straight-forward and well-established, methods for determining water requirements for the in stream uses have been developed only recently and are continuing to evolve. Water laws that have favored the more traditional water uses, the inherent nature of conflict between instream and offstream water uses, and the special kinds of technological and philosophical problems posed by the "newer" types of instream uses are described below. Water laws that have been passed to accommodate the more recently recognized instream uses are summarized.

Report↗

Using GIS to assess priorities of infrastructure and health needs of colonias along the United States-Mexico border

Colonias, which are unincorporated border setdements in the United. States, have emerged in rural areas without the governance and services normally provided by local government. Colonia residents live in poverty and lack adequate health care, potable water, and sanitation systems. These conditions create substantial health risks for themselves and surrounding communities. By 2001, more than 1,400 colonias were identified in Texas. Cooperation with several Federal and Texas state agencies has allowed the U.S. Geological Survey (USGS) to improve colonia Geographic Information System (GIS) boundaries and develop the Colonia Health, Infrastructure, and Platting Status tool (CHIPS). Together, the GIS boundaries and CHIPS aid the Texas government in prioritizing the limited funds that are available for infrastructure improvement. CHIPS's report: generator can be tailored, to the needs of the user, providing either broad or specific output. CHIPS is publicly available on the U.S. Geological Survey Border Environmental Health Initiative website at http://borderhealth.cr. usgs.gov.

Journal of Latin American Geography↗

Metallogeny, exploitation and environmental impact of the Mt. Amiata mercury ore district (Southern Tuscany, Italy)

The Mt. Amiata mining district (Southern Tuscany, Italy) is a world class Hg district, with a cumulate production of more than 100,000 tonnes of Hg, mostly occurring between 1870 and 1980. The Hg mineralization at Mt. Amiata is younger than 0.3 Ma, and is directly related to shallow hydrothermal systems similar to present-day geothermal fields of the region. There is likely a continuum of Hg deposition to present day, because Hg emission from geothermal power plants is on-going. In this sense, the Mt. Amiata deposits present some analogies with &ldquo;hot-spring type&rdquo; deposits of western USA, although an ore deposit model for the district has not been established. Specifically, the source of Hg remains highly speculative. The mineralizing hydrothermal fluids are of low temperature, and of essentially meteoric origin. Recent results by our research group indicate that, 30 years after mine closure, the environmental effects of Hg contamination related to mining are still recorded by the ecosystem, namely on waterways of the Paglia and Tiber River basins. In particular, the close spatial connection between the town of Abbadia San Salvatore, the Hg mine within its immediate neighborhood, and the drainage catchment of the Paglia River has an influence also on Hg speciation, transported mainly in the particulate form by the river system. The extent of Hg contamination has been identified at least 100 km from Abbadia San Salvatore along the Paglia-Tiber River system. Estimated annual Hg mass loads transported by the Paglia River to the Tiber River were about 11 kg yr &minus;1 . However, there is evidence that flood events may enhance Hg mobilization in the Paglia River basin, increasing Hg concentrations in stream sediment. The high methyl-Hg/Hg ratio in water in this area is an additional factor of great concern due to the potential harmful effects on human and wildlife health. Results of our studies indicate that the Mt. Amiata region is at present a source of Hg of remarkable environmental concern at the local, regional (Tiber River), and Mediterranean scales. Ongoing studies are aimed to a more detailed quantification of the Hg mass load input to the Mediterranean Sea, and to unravel the processes concerning Hg transport and fluid dynamics.

Tuscany↗